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19 pages, 4365 KB  
Article
Enhancing Load Stratification in Power Distribution Systems Through Clustering Algorithms: A Practical Study
by Williams Mendoza-Vitonera, Xavier Serrano-Guerrero, María-Fernanda Cabrera, John Enriquez-Loja and Antonio Barragán-Escandón
Energies 2025, 18(19), 5314; https://doi.org/10.3390/en18195314 - 9 Oct 2025
Abstract
Accurate load profile identification is crucial for effective and sustainable power system planning. This study proposes a characterization methodology based on clustering techniques applied to consumption data from medium- and low-voltage users, as well as distribution transformers from an electric utility. Three algorithms—K-means, [...] Read more.
Accurate load profile identification is crucial for effective and sustainable power system planning. This study proposes a characterization methodology based on clustering techniques applied to consumption data from medium- and low-voltage users, as well as distribution transformers from an electric utility. Three algorithms—K-means, DBSCAN (Density-Based Spatial Clustering of Applications with Noise), and Gaussian Mixture Models (GMM)—were implemented and compared in terms of their ability to form representative strata using variables such as observation count, projected energy, load factor (LF), and characteristic power levels. The methodology includes data cleaning, normalization, dimensionality reduction, and quality metric analysis to ensure cluster consistency. Results were benchmarked against a prior study conducted by Empresa Eléctrica Regional Centro Sur C.A. (EERCS). Among the evaluated algorithms, GMM demonstrated superior performance in modeling irregular consumption patterns and probabilistically assigning observations, resulting in more coherent and representative segmentations. The resulting clusters exhibited an average LF of 58.82%, indicating balanced demand distribution and operational consistency across the groups. Compared to alternative clustering techniques, GMM demonstrated advantages in capturing heterogeneous consumption patterns, adapting to irregular load behaviors, and identifying emerging user segments such as induction-cooking households. These characteristics arise from its probabilistic nature, which provides greater flexibility in cluster formation and robustness in the presence of variability. Therefore, the findings highlight the suitability of GMM for real-world applications where representativeness, efficiency, and cluster stability are essential. The proposed methodology supports improved transformer sizing, more precise technical loss assessments, and better demand forecasting. Periodic application and integration with predictive models and smart grid technologies are recommended to enhance strategic and operational decision-making, ultimately supporting the transition toward smarter and more resilient power distribution systems. Full article
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20 pages, 4879 KB  
Article
Study on the Influence of Mesoscopic Parameters on Proppant Crushing Performance Based on the Particle Flow Method
by Yi Zou, Desheng Zhou, Yufei Wang, Chen Lu, Haiyang Wang and Qingqing Wang
Processes 2025, 13(10), 3188; https://doi.org/10.3390/pr13103188 - 8 Oct 2025
Abstract
Proppant crushing seriously affects the efficiency and effectiveness of oil and gas production. In conventional studies, multi-particle crushing research often adopts the particle replacement method; however, this method results in a relatively rough and discontinuous crushing simulation process, making energy conservation difficult to [...] Read more.
Proppant crushing seriously affects the efficiency and effectiveness of oil and gas production. In conventional studies, multi-particle crushing research often adopts the particle replacement method; however, this method results in a relatively rough and discontinuous crushing simulation process, making energy conservation difficult to maintain before and after crushing, neglects complex mechanical behaviors such as internal stress distribution and crack propagation of particles, and thus lacks mechanical authenticity. Thus, this study employs the bonded crushing method and establishes a calibration method for mesoscopic parameters. By constructing a particle flow numerical model, the force and crushing processes of proppants under different mesoscopic parameter conditions for both single-particle clusters and multi-particle clusters are simulated, enabling comprehensive monitoring of internal crack propagation within particle clusters. The study systematically analyzes and investigates the influence of key mesoscopic parameters including the tensile strength of parallel bonds (pb-ten), cohesion of parallel bonds (pb-coh), effective modulus (emod), and stiffness ratio (kratio) on the maximum force required for particle crushing. Additionally, orthogonal experiment analysis is used to study the influence of different mesoscopic parameters on the proppant crushing rate. The results show that the larger the pb-ten and pb-coh, the less likely the proppant particle clusters are to crush; conversely, the higher the emod, the more likely the particle clusters are to crush. Within a certain range, pb-ten has the most significant impact on the proppant crushing rate, followed by pb-coh and emod, while kratio has a smaller impact. Based on the research results regarding the influence of laws of different mesoscopic parameters on proppant crushing performance, the mesoscopic parameters of the proppant were calibrated using the post-experiment proppant crushing rate as the fitting index. The simulation results were then compared with the experimental results, verifying the accuracy of the model. The findings of this study clarify the influence of laws of mesoscopic parameters on proppant crushing performance, providing a basis for the subsequent calibration of mesoscopic parameters for numerical proppants and helping to accurately characterize the macroscopic crushing performance of numerical proppants. Full article
(This article belongs to the Section Particle Processes)
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22 pages, 3656 KB  
Article
Design and Experimental Validation of a Cluster-Based Virtual Power Plant with Centralized Management System in Compliance with IEC Standard
by Putu Agus Aditya Pramana, Akhbar Candra Mulyana, Khotimatul Fauziah, Hafsah Halidah, Sriyono Sriyono, Buyung Sofiarto Munir, Yusuf Margowadi, Dionysius Aldion Renata, Adinda Prawitasari, Annisaa Taradini, Arief Kurniawan and Kholid Akhmad
Energies 2025, 18(19), 5300; https://doi.org/10.3390/en18195300 - 7 Oct 2025
Abstract
As power systems decentralize, Virtual Power Plants (VPPs) offer a promising approach to coordinate distributed energy resources (DERs) and enhance grid flexibility. However, real-world validation of VPP performance in Indonesia remains limited, especially regarding internationally aligned test standards. This study presents the design [...] Read more.
As power systems decentralize, Virtual Power Plants (VPPs) offer a promising approach to coordinate distributed energy resources (DERs) and enhance grid flexibility. However, real-world validation of VPP performance in Indonesia remains limited, especially regarding internationally aligned test standards. This study presents the design and experimental validation of a cluster-based VPP framework integrated with a centralized VPP Management System (VMS). Each cluster integrates solar photovoltaic (PV) system, battery energy storage system (BESS), and controllable load. A Local Control Unit (LCU) manages cluster operations, while the VMS coordinates power export–import dispatch, cluster-level aggregation, and grid compliance. The framework proposes a scalable VPP architecture and presents the first comprehensive experimental verification of key VPP performance indicators, including response time, adjustment rate, and accuracy, in the Indonesian context. Testing was conducted in alignment with the IEC TS 63189-1:2023 international standard. Results suggest real time responsiveness and indicate that, even at smaller scales, VPPs may contribute effectively to voltage control while exhibiting minimal influence on system frequency in interconnected grids. These findings confirm the capability of the proposed VPP framework to provide reliable real time control, ancillary services, and aggregated energy management. Its cluster-based architecture supports scalability for broader deployment in complex grid environments. Full article
(This article belongs to the Section F2: Distributed Energy System)
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75 pages, 13041 KB  
Article
Decarbonizing the Building Sector: The Integrated Role of Environmental, Social, and Governance Indicators
by Nicola Magaletti, Valeria Notarnicola, Mauro Di Molfetta and Angelo Leogrande
Buildings 2025, 15(19), 3601; https://doi.org/10.3390/buildings15193601 - 7 Oct 2025
Abstract
Climate change mitigation for the built environment has become a subject of greatest urgency, as buildings account for nearly 40% of total energy consumption and nearly one-third of total CO2 emissions. While environmental, social, and governance (ESG) indicators are increasingly used to [...] Read more.
Climate change mitigation for the built environment has become a subject of greatest urgency, as buildings account for nearly 40% of total energy consumption and nearly one-third of total CO2 emissions. While environmental, social, and governance (ESG) indicators are increasingly used to monitor sustainability performance, their collective role in impacting building-related emissions is yet largely under-investigated. The current research closes that gap through an examination of the ESG dimension–CO2 emissions intersection of 180 nations from 2000 to 2022, in the hope of illuminating how environmental, social, and governance elements interact to facilitate decarbonization. The research is guided by a multi-method design, including econometric examination, cluster modeling, and machine learning techniques, which provide causal evidence and predictive analysis, respectively. The findings reveal that the deployment of renewable energy significantly reduces emissions, while per capita energy use and PM2.5 air pollution exacerbate this effect. The social indicators show mixed results: learning, women’s parliamentary representation, and women’s workforce representation reduce emissions, while food production and growth among the lowest-income individuals demonstrate higher emissions. Governance demonstrates mixed results as well, with good regulation reducing emissions under specific conditions yet primarily supporting high-income countries with superior infrastructure. The examination of clusters reveals that ESG-balanced performance is retained by countries in the low-emission clusters, whereas decentralized ESG pillars are associated with higher emissions. Machine learning confirms the existence of non-linear effects and identifies PM2.5 exposure and renewable energy deployment as the strongest predictors of the relationship. In summary, the findings suggest that successful policies for decarbonizing the built environment are constructed upon the consistency of environmental, social, and governance plans, rather than single steps. Full article
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27 pages, 1706 KB  
Article
An End-to-End Framework for Spatiotemporal Data Recovery and Unsupervised Cluster Partitioning in Distributed PV Systems
by Bingxu Zhai, Yuanzhuo Li, Wei Qiu, Rui Zhang, Zhilin Jiang, Yinuo Zeng, Tao Qian and Qinran Hu
Processes 2025, 13(10), 3186; https://doi.org/10.3390/pr13103186 (registering DOI) - 7 Oct 2025
Abstract
The growing penetration of distributed photovoltaic (PV) systems presents significant operational challenges for power grids, driven by the scarcity of historical data and the high spatiotemporal variability of PV generation. To address these challenges, we propose Generative Reconstruction and Adaptive Identification via Latents [...] Read more.
The growing penetration of distributed photovoltaic (PV) systems presents significant operational challenges for power grids, driven by the scarcity of historical data and the high spatiotemporal variability of PV generation. To address these challenges, we propose Generative Reconstruction and Adaptive Identification via Latents (GRAIL), a unified, end-to-end framework that integrates generative modeling with adaptive clustering to discover latent structures and representative scenarios in PV datasets. GRAIL operates through a closed-loop mechanism where clustering feedback guides a cluster-aware data generation process, and the resulting generative augmentation strengthens partitioning in the latent space. Evaluated on a real-world, multi-site PV dataset with a high missing data rate of 45.4%, GRAIL consistently outperforms both classical clustering algorithms and deep embedding-based methods. Specifically, GRAIL achieves a Silhouette Score of 0.969, a Calinski–Harabasz index exceeding 4.132×106, and a Davies–Bouldin index of 0.042, demonstrating superior intra-cluster compactness and inter-cluster separation. The framework also yields a normalized entropy of 0.994, which indicates highly balanced partitioning. These results underscore that coupling data generation with clustering is a powerful strategy for expressive and robust structure learning in data-sparse environments. Notably, GRAIL achieves significant performance gains over the strongest deep learning baseline that lacks a generative component, securing the highest composite score among all evaluated methods. The framework is also computationally efficient. Its alternating optimization converges rapidly, and clustering and reconstruction metrics stabilize within approximately six iterations. Beyond quantitative performance, GRAIL produces physically interpretable clusters that correspond to distinct weather-driven regimes and capture cross-site dependencies. These clusters serve as compact and robust state descriptors, valuable for downstream applications such as PV forecasting, dispatch optimization, and intelligent energy management in modern power systems. Full article
(This article belongs to the Section Energy Systems)
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24 pages, 24720 KB  
Article
Parallel Rendezvous Strategy for Node Association in Wi-SUN FAN Networks
by Ananias Ambrosio Quispe, Rodrigo Jardim Riella, Luciana Michelotto Iantorno, Patryk Henrique da Fonseca, Vitalio Alfonso Reguera and Evelio Martin Garcia Fernandez
Sensors 2025, 25(19), 6213; https://doi.org/10.3390/s25196213 - 7 Oct 2025
Abstract
The Wi-SUN FAN (Wireless Smart Ubiquitous Network Field Area Network) standard facilitates large-scale connectivity among smart devices in utility networks and smart cities. Specifically designed for Low-Power and Lossy Networks (LLNs), Wi-SUN FAN supports the formation of multiple Personal Area Networks (PANs) and [...] Read more.
The Wi-SUN FAN (Wireless Smart Ubiquitous Network Field Area Network) standard facilitates large-scale connectivity among smart devices in utility networks and smart cities. Specifically designed for Low-Power and Lossy Networks (LLNs), Wi-SUN FAN supports the formation of multiple Personal Area Networks (PANs) and mesh topologies with multi-hop transmissions. However, the node association process, divided into five junction states, often results in prolonged connection times, particularly in multi-hop networks, thereby limiting network scalability and reliability. This study analyzes the factors affecting these delays, with a particular focus on Join State 1 (JS1), which relies on PAN Advertisement (PA) packets that use asynchronous communication and the trickle timer algorithm, frequently causing significant delays. To overcome this challenge in JS1, we propose the Parallel Rendezvous (PR) strategy, which forms synchronized clusters of unassociated nodes and leverages the standard’s PAN Advertisement Solicit (PAS) packets to rapidly disseminate network information. The proposed algorithm, PR Wi-SUN FAN, is evaluated through simulations in various network topologies, demonstrating notable improvements in linear, fully connected, and mesh scenarios. The most significant gains are observed in the linear topology, with reductions of up to 71.22% in association time and 59.56% in energy consumption during JS1. Full article
(This article belongs to the Section Intelligent Sensors)
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23 pages, 3751 KB  
Article
DAF-Aided ISAC Spatial Scattering Modulation for Multi-Hop V2V Networks
by Yajun Fan, Jiaqi Wu, Yabo Guo, Jing Yang, Le Zhao, Wencai Yan, Shangjun Yang, Haihua Ma and Chunhua Zhu
Sensors 2025, 25(19), 6189; https://doi.org/10.3390/s25196189 - 6 Oct 2025
Viewed by 168
Abstract
Integrated sensing and communication (ISAC) has emerged as a transformative technology for intelligent transportation systems. Index modulation (IM), recognized for its high robustness and energy efficiency (EE), has been successfully incorporated into ISAC systems. However, most existing IM-based ISAC schemes overlook the spatial [...] Read more.
Integrated sensing and communication (ISAC) has emerged as a transformative technology for intelligent transportation systems. Index modulation (IM), recognized for its high robustness and energy efficiency (EE), has been successfully incorporated into ISAC systems. However, most existing IM-based ISAC schemes overlook the spatial multiplexing potential of millimeter-wave channels and remain confined to single-hop vehicle-to-vehicle (V2V) setups, failing to address the challenges of energy consumption and noise accumulation in real-world multi-hop V2V networks with complex road topologies. To bridge this gap, we propose a spatial scattering modulation-based ISAC (ISAC-SSM) scheme and introduce it to multi-hop V2V networks. The proposed scheme leverages the sensed positioning information to select maximum signal-to-noise ratio relay vehicles and employs a detect-amplify-and-forward (DAF) protocol to mitigate noise propagation, while utilizing sensed angle data for Doppler compensation to enhance communication reliability. At each hop, the transmitter modulates index bits on the angular-domain spatial directions of scattering clusters, achieving higher EE. We initially derive a closed-form bit error rate expression and Chernoff upper bound for the proposed DAF ISAC-SSM under multi-hop V2V networks. Both theoretical analyses and Monte Carlo simulations have been made and demonstrate the superiority of DAF ISAC-SSM over existing alternatives in terms of EE and error performance. Specifically, in a two-hop network with 12 scattering clusters, compared with DAF ISAC-conventional spatial multiplexing, DAF ISAC-maximum beamforming, and DAF ISAC-random beamforming, the proposed DAF ISAC-SSM scheme can achieve a coding gain of 1.5 dB, 2 dB, and 4 dB, respectively. Moreover, it shows robust performance with less than a 1.5 dB error degradation under 0.018 Doppler shifts, thereby verifying its superiority in practical vehicular environments. Full article
36 pages, 4435 KB  
Article
Federated Reinforcement Learning with Hybrid Optimization for Secure and Reliable Data Transmission in Wireless Sensor Networks (WSNs)
by Seyed Salar Sefati, Seyedeh Tina Sefati, Saqib Nazir, Roya Zareh Farkhady and Serban Georgica Obreja
Mathematics 2025, 13(19), 3196; https://doi.org/10.3390/math13193196 - 6 Oct 2025
Viewed by 107
Abstract
Wireless Sensor Networks (WSNs) consist of numerous battery-powered sensor nodes that operate with limited energy, computation, and communication capabilities. Designing routing strategies that are both energy-efficient and attack-resilient is essential for extending network lifetime and ensuring secure data delivery. This paper proposes Adaptive [...] Read more.
Wireless Sensor Networks (WSNs) consist of numerous battery-powered sensor nodes that operate with limited energy, computation, and communication capabilities. Designing routing strategies that are both energy-efficient and attack-resilient is essential for extending network lifetime and ensuring secure data delivery. This paper proposes Adaptive Federated Reinforcement Learning-Hunger Games Search (AFRL-HGS), a Hybrid Routing framework that integrates multiple advanced techniques. At the node level, tabular Q-learning enables each sensor node to act as a reinforcement learning agent, making next-hop decisions based on discretized state features such as residual energy, distance to sink, congestion, path quality, and security. At the network level, Federated Reinforcement Learning (FRL) allows the sink node to aggregate local Q-tables using adaptive, energy- and performance-weighted contributions, with Polyak-based blending to preserve stability. The binary Hunger Games Search (HGS) metaheuristic initializes Cluster Head (CH) selection and routing, providing a well-structured topology that accelerates convergence. Security is enforced as a constraint through a lightweight trust and anomaly detection module, which fuses reliability estimates with residual-based anomaly detection using Exponentially Weighted Moving Average (EWMA) on Round-Trip Time (RTT) and loss metrics. The framework further incorporates energy-accounted control plane operations with dual-format HELLO and hierarchical ADVERTISE/Service-ADVERTISE (SrvADVERTISE) messages to maintain the routing tables. Evaluation is performed in a hybrid testbed using the Graphical Network Simulator-3 (GNS3) for large-scale simulation and Kali Linux for live adversarial traffic injection, ensuring both reproducibility and realism. The proposed AFRL-HGS framework offers a scalable, secure, and energy-efficient routing solution for next-generation WSN deployments. Full article
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27 pages, 788 KB  
Article
Supply Chain Ecosystem for Smart Sustainable City Multifloor Manufacturing Cluster: Knowledge Management Based on Open Innovation and Energy Conservation Policies
by Tygran Dzhuguryan, Kinga Kijewska, Stanisław Iwan and Karina Dzhuguryan
Sustainability 2025, 17(19), 8882; https://doi.org/10.3390/su17198882 - 6 Oct 2025
Viewed by 136
Abstract
City manufacturing (CM) is a key concept in smart sustainable cities. City multifloor manufacturing clusters (CMFMCs) are an integral part of large urban areas. Although smart sustainable CMFMCs attract growing attention, a major research gap remains. It concerns how different actors drive innovation [...] Read more.
City manufacturing (CM) is a key concept in smart sustainable cities. City multifloor manufacturing clusters (CMFMCs) are an integral part of large urban areas. Although smart sustainable CMFMCs attract growing attention, a major research gap remains. It concerns how different actors drive innovation within their supply chain ecosystems (SCEs). To address this gap, this paper examines the SCE of a CMFMC and knowledge management (KM) mechanisms of open innovation (OI), considering energy conservation (EC) policies. This qualitative study expands the understanding of the spatial configuration and key actors of the SCE of a CMFMC. It also analyses the role of the University Centre for Projects and Innovation (UCPI) as a physical orchestrator. The UCPI fosters innovation activity through KM based on OI and EC. Our findings contribute to the SCE literature by emphasizing the potential of its key actors. We show that an integrated approach to KM based on OI and EC enhances innovation in CMFMCs. This supports the sustainable development of smart cities. Full article
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28 pages, 3571 KB  
Article
Methodology for Transient Stability Assessment and Enhancement in Low-Inertia Power Systems Using Phasor Measurements: A Data-Driven Approach
by Mihail Senyuk, Svetlana Beryozkina, Ismoil Odinaev, Inga Zicmane and Murodbek Safaraliev
Mathematics 2025, 13(19), 3192; https://doi.org/10.3390/math13193192 - 5 Oct 2025
Viewed by 223
Abstract
Modern energy systems are undergoing a profound transformation characterized by the active replacement of conventional fossil-fuel-based power plants with renewable energy sources. This transition aims to reduce the carbon emissions associated with electricity generation while enhancing the economic performance of electric power market [...] Read more.
Modern energy systems are undergoing a profound transformation characterized by the active replacement of conventional fossil-fuel-based power plants with renewable energy sources. This transition aims to reduce the carbon emissions associated with electricity generation while enhancing the economic performance of electric power market players. However, alongside these benefits come several challenges, including reduced overall inertia within energy systems, heightened stochastic variability in grid operation regimes, and stricter demands on the rapid response capabilities and adaptability of emergency controls. This paper presents a novel methodology for selecting effective control laws for low-inertia energy systems, ensuring their dynamic stability during post-emergency operational conditions. The proposed approach integrates advanced techniques, including feature selection via decision tree algorithms, classification using Random Forest models, and result visualization through the Mean Shift clustering method applied to a two-dimensional representation derived from the t-distributed Stochastic Neighbor Embedding technique. A modified version of the IEEE39 benchmark model served as the testbed for numerical experiments, achieving a classification accuracy of 98.3%, accompanied by a control law synthesis delay of just 0.047 milliseconds. In conclusion, this work summarizes the key findings and outlines potential enhancements to refine the presented methodology further. Full article
(This article belongs to the Special Issue Mathematical Applications in Electrical Engineering, 2nd Edition)
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40 pages, 4433 KB  
Article
Economic Convergence Analyses in Perspective: A Bibliometric Mapping and Its Strategic Implications (1982–2025)
by Geisel García-Vidal, Néstor Alberto Loredo-Carballo, Reyner Pérez-Campdesuñer and Gelmar García-Vidal
Economies 2025, 13(10), 289; https://doi.org/10.3390/economies13100289 - 4 Oct 2025
Viewed by 301
Abstract
This study presents a bibliometric and thematic analysis of economic convergence analysis from 1982 to 2025, based on a corpus of 2924 Scopus-indexed articles. Using VOSviewer and the bibliometrix R package, this research maps the field’s intellectual structure, identifying five main thematic clusters: [...] Read more.
This study presents a bibliometric and thematic analysis of economic convergence analysis from 1982 to 2025, based on a corpus of 2924 Scopus-indexed articles. Using VOSviewer and the bibliometrix R package, this research maps the field’s intellectual structure, identifying five main thematic clusters: (1) formal statistical models, (2) institutional-contextual approaches, (3) theoretical–statistical foundations, (4) nonlinear historical dynamics, and (5) normative and policy assessments. These reflect a shift from descriptive to explanatory and prescriptive frameworks, with growing integration of sustainability, spatial analysis, and institutional factors. The most productive journals include Journal of Econometrics (121 articles), Applied Economics (117), and Journal of Cleaner Production (81), while seminal contributions by Quah, Im et al., and Levin et al. anchor the co-citation network. International collaboration is significant, with 25.99% of publications involving cross-country co-authorship, particularly in European and North American networks. The field has grown at a compound annual rate of 14.4%, accelerating after 2000 and peaking in 2022–2024, indicating sustained academic interest. These findings highlight the maturation of convergence analysis as a multidisciplinary domain. Practically, this study underscores the value of composite indicators and spatial econometric models for monitoring regional, environmental, and technological convergence—offering policymakers tools for inclusive growth, climate resilience, and innovation strategies. Moreover, the emergence of clusters around sustainability and digital transformation reveals fertile ground for future research at the intersection of transitions in energy, digital, and institutional domains and sustainable development (a broader sense of structural change). Full article
(This article belongs to the Special Issue Regional Economic Development: Policies, Strategies and Prospects)
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14 pages, 9301 KB  
Article
Insights into Cold-Season Adaptation of Mongolian Wild Asses Revealed by Gut Microbiome Metagenomics
by Jianeng Wang, Haifeng Gu, Hongmei Gao, Tongzuo Zhang, Feng Jiang, Pengfei Song, Yan Liu, Qing Fan, Youjie Xu and Ruidong Zhang
Microorganisms 2025, 13(10), 2304; https://doi.org/10.3390/microorganisms13102304 - 4 Oct 2025
Viewed by 216
Abstract
The Mongolian wild ass (Equus hemionus hemionus) is a flagship species of the desert-steppe ecosystem in Asia, and understanding its strategies for coping with cold environments is vital for both revealing its survival mechanisms and informing conservation efforts. In this study, [...] Read more.
The Mongolian wild ass (Equus hemionus hemionus) is a flagship species of the desert-steppe ecosystem in Asia, and understanding its strategies for coping with cold environments is vital for both revealing its survival mechanisms and informing conservation efforts. In this study, we employed metagenomic sequencing to characterize the composition and functional potential of the gut microbiota, and applied DNA metabarcoding of the chloroplast trnL (UAA) g–h fragment to analyze dietary composition, aiming to reveal seasonal variations and the interplay between dietary plant composition and gut microbial communities. In the cold season, Bacteroidota and Euryarchaeota were significantly enriched, suggesting enhanced fiber degradation and energy extraction from low-quality forage. Moreover, genera such as Bacteroides and Alistipes were also significantly enriched and associated with short-chain fatty acid (SCFA) metabolism, bile acid tolerance, and immune modulation. In the cold season, higher Simpson index values and tighter principal coordinates analysis (PCoA) clustering indicated a more diverse and stable microbiota under harsh environmental conditions, which may represent an important microecological strategy for the host to cope with extreme environments. Functional predictions based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) further indicated upregulation of metabolic and signaling pathways, including ABC transporters, two-component systems, and quorum sensing, suggesting multi-level microbial responses to low temperatures and nutritional stress. trnL-based plant composition analysis indicated seasonal shifts, with Tamaricaceae detected more in the warm season and Poaceae, Chenopodiaceae, and Amaryllidaceae detected more in the cold season. Correlation analyses revealed that dominant microbial phyla were associated with the degradation of fiber, polysaccharides, and plant secondary metabolites, which may help maintain host energy and metabolic homeostasis. Despite the limited sample size and cross-sectional design, our findings highlight that gut microbial composition and structure may be important for host adaptation to cold environments and may also serve as a useful reference for future studies on the adaptive mechanisms and conservation strategies of endangered herbivores, including the Mongolian wild ass. Full article
(This article belongs to the Section Gut Microbiota)
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20 pages, 1670 KB  
Article
Exploring Bone Health Determinants in Youth Athletes Using Supervised and Unsupervised Machine Learning
by Nikolaos-Orestis Retzepis, Alexandra Avloniti, Christos Kokkotis, Theodoros Stampoulis, Dimitrios Balampanos, Dimitrios Draganidis, Anastasia Gkachtsou, Marietta Grammenou, Anastasia Maria Karaiskou, Danai Kelaraki, Maria Protopapa, Dimitrios Pantazis, Maria Emmanouilidou, Panagiotis Aggelakis, Nikolaos Zaras, Ilias Smilios, Ioannis G. Fatouros, Maria Michalopoulou and Athanasios Chatzinikolaou
Dietetics 2025, 4(4), 44; https://doi.org/10.3390/dietetics4040044 - 4 Oct 2025
Viewed by 118
Abstract
Background: Bone health in youth is influenced by both modifiable factors, such as nutrition and physical activity, and non-modifiable factors, such as biological maturation and heredity. Understanding how these elements interact to predict body composition may enhance the effectiveness of early interventions. Importantly, [...] Read more.
Background: Bone health in youth is influenced by both modifiable factors, such as nutrition and physical activity, and non-modifiable factors, such as biological maturation and heredity. Understanding how these elements interact to predict body composition may enhance the effectiveness of early interventions. Importantly, the integration of both supervised and unsupervised machine learning models enables a data-driven exploration of complex relationships, allowing for accurate prediction and subgroup discovery. Methods: This cross-sectional study examined 94 male athletes during the developmental period. Anthropometric, performance, and nutritional data were collected, and bone parameters were assessed using dual-energy X-ray absorptiometry (DXA). Three supervised machine learning models (Random Forest, Gradient Boosting, and Support Vector Regression) were trained to predict Total Body-Less Head (TBLH) values. Nested cross-validation assessed model performance. Unsupervised clustering (K-Means) was also applied to identify dietary intake profiles (calcium, protein, vitamin D). SHAP analysis was used for model interpretability. Results: The Random Forest model yielded the best predictive performance (R2 = 0.71, RMSE = 0.057). Weight, height, and handgrip strength were the most influential predictors. Clustering analysis revealed two distinct nutritional profiles; however, t-tests showed no significant differences in TBLH or regional BMD between the clusters. Conclusions: Machine learning, both supervised for accurate prediction and unsupervised for nutritional subgroup discovery, provides a robust, interpretable framework for assessing adolescent bone health. While dietary intake clusters did not align with significant differences in bone parameters, this finding underscores the multifactorial nature of skeletal development and highlights areas for further exploration. Full article
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14 pages, 2518 KB  
Article
Assessment of Intervertebral Lumbar Disk Herniation: Accuracy of Dual-Energy CT Compared to MRI
by Giuseppe Ocello, Gianluca Tripodi, Flavio Spoto, Leonardo Monterubbiano, Gerardo Serra, Giorgio Merci and Giovanni Foti
J. Clin. Med. 2025, 14(19), 7000; https://doi.org/10.3390/jcm14197000 - 3 Oct 2025
Viewed by 202
Abstract
Background: Lumbar disk herniation is a common cause of low back pain and radiculopathy, significantly impacting patients’ life quality and functional capacity. Magnetic Resonance Imaging (MRI) remains the gold standard for its assessment due to its superior soft tissue contrast and multiplanar imaging [...] Read more.
Background: Lumbar disk herniation is a common cause of low back pain and radiculopathy, significantly impacting patients’ life quality and functional capacity. Magnetic Resonance Imaging (MRI) remains the gold standard for its assessment due to its superior soft tissue contrast and multiplanar imaging capabilities. However, recent advances in spectral computed tomography (CT), particularly dual-energy CT (DECT), have introduced new diagnostic opportunities, offering improved soft tissue characterization. Objective: To evaluate the diagnostic performance of DECT in detecting and grading lumbar disk herniations using dedicated color-coded fat maps. Materials and Methods: A total of 205 intervertebral levels from 41 consecutive patients with lumbar symptoms were prospectively analyzed. All patients underwent both DECT and MRI within 3 days. Three radiologists with varying years of experience independently assessed DECT images using color-coded reconstructions. A five-point grading score was attributed to each lumbar level: 1 = normal disk, 2 = bulging/protrusion, 3 = focal herniation, 4 = extruded herniation, and 5 = migrated fragment. The statistical analysis included Pearson’s correlation for score consistency, Cohen’s Kappa for interobserver agreement, generalized estimating equations for a cluster-robust analysis, and an ROC curve analysis. The DECT diagnostic accuracy was assessed in a dichotomized model (grades 1–2 = no herniation; 3–5 = herniation), using MRI as reference. Results: A strong correlation was observed between DECT and MRI scores across all readers (mean Pearson’s r = 0.826, p < 0.001). The average exact agreement between DECT and MRI was 79.4%, with the highest concordance at L1–L2 (86.7%) and L5–S1 (80.4%). The interobserver agreement was substantial (mean Cohen’s κ = 0.765), with a near-perfect agreement between the two most experienced readers (κ = 0.822). The intraclass correlation coefficient was 0.906 (95% CI: 0.893–0.918). The ROC analysis showed excellent performance (AUC range: 0.953–0.986). In the dichotomous model, DECT demonstrated a markedly higher sensitivity than conventional CT (95.1% vs. 57.2%), with a comparable specificity (DECT: 99.0%; CT: 96.5%) and improved overall accuracy (98.4% vs. 90.0%). Subgroup analyses by age and disk location revealed no statistically significant differences. Conclusions: The use of DECT dedicated color-coded fat map reconstructions showed high diagnostic performance in the assessment of lumbar disk herniations compared to MRI. These findings support the development of dedicated post-processing tools, facilitating the broader clinical adoption of spectral CT, especially in cases where MRI is contraindicated or less accessible. Full article
(This article belongs to the Special Issue Dual-Energy and Spectral CT in Clinical Practice: 2nd Edition)
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Article
Temporal Segmentation of Urban Water Consumption Patterns Based on Non-Parametric Density Clustering
by Aliaksey A. Kapanski, Roman V. Klyuev, Vladimir S. Brigida and Nadezeya V. Hruntovich
Technologies 2025, 13(10), 449; https://doi.org/10.3390/technologies13100449 - 3 Oct 2025
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Abstract
The management of modern water supply systems requires a detailed analysis of consumption patterns in order to optimize pump operation schedules, reduce energy costs, and support the development of intelligent management systems. Traditional clustering algorithms are applied for these tasks; however, their limitation [...] Read more.
The management of modern water supply systems requires a detailed analysis of consumption patterns in order to optimize pump operation schedules, reduce energy costs, and support the development of intelligent management systems. Traditional clustering algorithms are applied for these tasks; however, their limitation lies in the need to predefine the number of clusters. The aim of this study was to develop and validate a non-parametric method for clustering daily water consumption profiles based on a modified DBSCAN algorithm. The proposed approach includes the automatic optimization of neighborhood radius and the minimum number of points required to form a cluster. The input data consisted of half-hourly water supply and electricity consumption values for the water supply system of Gomel (Republic of Belarus), supplemented with the time-of-day factor. As a result of the multidimensional clustering, two stable regimes were identified: a high-demand regime (6:30–22:30), covering about 46% of the data and accounting for more than half of the total water supply and electricity consumption, and a low-demand regime (0:30–6:00), representing about 21% of the data and forming around 15% of the resources. The remaining regimes reflect transitional states in morning and evening periods. The obtained results make it possible to define the temporal boundaries of the regimes and to use them for data labeling in the development of predictive water consumption models. Full article
(This article belongs to the Special Issue Sustainable Water and Environmental Technologies of Global Relevance)
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