Search Results (73)

This study examines seasonal changes in muscle and heart parameters in eventing horses over the course of a competition season. Blood levels of the enzymes creatine kinase (CK) and aspartate aminotransferase (AST) as well as the heart muscle-specific concentration of cardiac troponin I (cTnI) were measured before (pre), 30 min (p30) and 24 h (p24) after competitions. Creatine kinase (CK: median pre-competition = 175 U/L, 30 min post = 221 U/L, 24 h post = 140 U/L), aspartate aminotransferase (AST: pre = 319 U/L, p30 = 335 U/L, p24 = 333 U/L), and cardiac troponin I concentration (cTnI: pre = 0.006 ng/mL, p30 = 0.011 ng/mL, p24 = 0.007 ng/mL) exhibited partial normalization by 24 h post-exercise but at the same time demonstrated significant seasonal variation (p < 0.001). Echocardiographic assessments revealed sustained high-level myocardial velocities, with occasional modest seasonal declines. A significant correlation was identified between cTnI levels and early diastolic myocardial velocity (Em) (Spearman’s Rho: pre-exercise 0.323, Rho p30: 0.357), whereas a negative correlation was manifest at 24 h (Spearman’s Rho = −0.300). These findings suggest a heightened sensitivity of diastolic myocardial velocity to myocardial injury. Given that diastolic dysfunction frequently constitutes an early manifestation of myocardial compromise, our results underscore the utility of biomarkers alongside myocardial velocity measures as valuable tools for the early detection of subclinical fatigue in high-performance sport horses. Full article

Polo is an equestrian sport with a long tradition, but to date there is little scientific data on health risks, particularly lameness. In an international online survey, 145 polo players (47.6% female, 52.4% male) with an average of 13.4 years of playing experience were questioned about lameness risks and influencing factors. The respondents reported a median of six horses, most frequently mares as their best-performing and healthiest animals. Horse age was significantly associated with the risk of lameness, increasing by approximately 19.5% per year (OR = 1.195; p = 0.030) and differed depending on the breed of horse. Player-related variables such as age, sex, level of experience and type of husbandry had no significant influence. Risk perception was only associated with the handicap: players with a higher handicap rated the risk of injury as lower. Sex, age and horse ownership were not relevant. Unfavourable ground conditions were cited as the most common cause of injury (58.6%). The results show that players believe key predictors of lameness, in particular the age and breed of the horses, are objective factors, while subjective risk assessments are primarily influenced by the players’ level of experience. Further studies are necessary to improve horse health in polo and to enable preventive measures. Full article

Bit pressure and rein tension-induced mouth pain in horses have recently become a significant welfare concern, fueling debates within the equestrian community and beyond. Evidence indicates that bits can cause pain-related behaviors and even oral lesions. Although studying pressure-induced pain in animals is challenging, the similarities in the physiology of pain processing (nociception) across mammals suggest that it is reasonable to assume that pain perception in humans and horses is principally comparable. Therefore, we compared human pressure pain detection thresholds (PPDTs) to reported rein pressures in equestrian sports as reported in the scientific literature. Reported rein tensions (kPa) range from a minimum of 91.2–107.87 to a maximum of 1314.09–4285.51, while human PPDTs (in kPa) are 232.4 for the face, 445.3 for the hand, and 535.5 for the foot. These comparisons reveal that only the lowest reported bit pressures would be pain-free for humans. Average to maximum pressures would cause strong to severe pain sensations in humans. Furthermore, data on pressure pain-induced stimulus response functions suggest that bit pressures commonly encountered in equestrian sports could cause lesions in humans, making them unacceptable in human experimentation. In conclusion, bit pressures as reported in the scientific literature would cause significant pain if applied to humans, raising welfare concerns for horses. Full article

The interaction between equine hooves and various ground surfaces is a critical factor for injury prevention and performance in modern equestrian sports. Accurate measurement of surface grip is essential for evaluating the effectiveness of different hoof protection systems. This study introduces the Vienna Grip Tester (VGT), a novel sensor-based device developed to quantify rotational resistance—an important parameter for assessing hoof–surface interaction. The VGT utilizes a torque wrench and spring-loaded mechanism to simulate lateral hoof movements under a standardized vertical load (~700 N), enabling objective grip measurements across different conditions. Twenty combinations of hoof protection (barefoot, traditional iron shoe, and two glue-on models) and surfaces (sand, sand with fiber at 25 °C and −18 °C, frozen sand, and turf) were tested, yielding 305 torque measurements. Statistical analysis (repeated-measures ANOVA with Bonferroni correction) revealed significant differences in grip among surface types and hoof protection systems. Frozen surfaces (SDAF (31 ± 8.9 Nm and SDF 33 ± 8.7 Nm, p < 0.001) exhibited the highest grip, while dry sand (SDA (18.3 ± 3.3 Nm, p < 0.001) showed the lowest. Glue-on shoes (glue-on grip, 26 ± 10 Nm; glue-on, 25 ± 10 Nm) consistently provided superior grip compared to traditional or unshod hooves (bare hoof, 21 ± 7 Nm). These results validate the VGT as a reliable and practical tool for measuring hoof–surface grip, with potential applications in injury prevention, hoof protection development, and surface optimization in equestrian sports. Full article

Bit-related trauma is a welfare issue in equestrian sports; therefore, a proper understanding of how the bit behaves inside the mouth is of importance. The objective of this study is to investigate the influence of bit design (snaffle bits/single/double-jointed; ring/eggbutt and curb bits), size, rein tension, and rein angle on the intra-oral movement of the bit in ex vivo specimens. The lateral and posterior movement of different bits was measured on cadaver heads under different circumstances. This ex vivo model, while not fully replicating natural conditions, provided insight into potential bit-related movements. Lateral displacement of the snaffle bit was significantly greater with higher unilateral rein tension. Bits that were too wide exhibited significantly more lateral and posterior displacement under unilateral rein tension. Posterior displacement increased with both unilateral and bilateral rein tensions. Using a bit that is too wide, using reins at a 20° angle, and applying 4 kg of rein tension were identified as risk factors for contact with the mesial gingival tissues of the mandibular 06s. Similar patterns were observed with curb bits, although bit movement was more restricted, and no contact with the mesial gingival tissues of the mandibular 06s was noted. This study demonstrated that bit design, bit size, rein angle, and rein tension all influence bit movement in the horse’s mouth. Full article

Equestrian sports play a significant economic role in the horse industry. In recent years, numerous equine viruses have emerged, among which are equine Pegiviruses and the re-emerging Equine coronavirus (ECoV). These viruses are distributed globally and primarily cause subclinical infections with unknown morbidity, even if ECoV can occasionally induce febrile and diarrheic episodes. To broaden the data on the Italian equine population, a study was conducted to assess their prevalence in two distinct horse populations belonging to the Carabinieri Corps (CC) and the Italian Army (IA) of the Italian Armed Forces (IAF). Samples consisted of blood serum and rectal swabs of 436 horses collected within the national surveillance program for equine infectious anemia and gastrointestinal parasite monitoring and analyzed for Pegivirus (caballi and equi) and ECoV by Real-Time RT PCR. The prevalence detected were 6.56% and 3.53%, respectively, for Pegivirus caballi and equi for the IA, while for the CC, they were 10.13% and 0.84%. Only one sample tested positive for ECoV belonging to a horse of the CC. Phylogenetic analyses were carried out on the PCR-positive samples that were sequenced using Sanger protocols. Understanding the epidemiology of these viruses is essential for evaluating the implementation of effective prevention strategies. Full article

Ecdysterone controls moulting and reproduction in insects, crustaceans, and helminths. It is also produced by many plants, probably as an insect deterrent. The steroid is not made by vertebrates but has anabolic effects in mammals and could be useful for treating sarcopenia in aged horses. However, ecdysterone is banned in horseracing and equestrian sports, and with no limit of reporting, the risk of unintended exposure to this naturally occurring prohibited substance is a concern. To explore this risk, pasture plants and hay samples were analysed for ecdysterone content, as well as samples of blood, faeces, and intestinal mucosa from horses (euthanized for non-research purposes) with varying degrees of endo-parasite infestation. The variability in serum ecdysterone concentrations between different horses after administering a fixed dose was also examined. Ecdysterone was detected in 24 hay samples (0.09 to 3.74 µg/g) and several weeds, with particularly high concentrations in Chenopodium album (244 µg/g) and Solanum nigrum (233 µg/g). There was a positive correlation between faecal ecdysterone and faecal egg counts, but no effect of anthelmintic treatment and no relation to the number of encysted cyathostome larvae in the large intestine mucosa. Certain horses maintained an unusually high serum ecdysterone concentration over several weeks and/or displayed an abnormally large response to oral ecdysterone administration. Thus, the risk of environmental exposure to ecdysterone is apparent, and several factors must be considered when determining an appropriate dosage for clinical studies or setting a reporting threshold for equine sports. Full article

This commentary evaluates contemporary equestrian sport governance through the lens of equine welfare science. Drawing on evidence from the FEI Sport Forum 2025 debates, the IFHA Racing Integrity Handbook, media coverage of horse sport, recent scientific presentations, regulatory texts, and published research, we identify systemic shortcomings in how horse welfare is assessed, prioritised, and protected. Key issues include reliance on performance as a proxy for welfare, inadequate “fit-to-compete” protocols, neglect of horses’ mental states, coercive tack and equipment practices (e.g., double bridles, tight nosebands, ear hoods), pharmacological and surgical interventions that mask pain, euphemistic regulatory language (e.g., whip “encouragement”), and inconsistent implementation of welfare rules. Through a series of case studies, from dressage and show jumping forums to racing integrity handbooks, we illustrate euphemistic language, defensive group dynamics, dismissive rhetoric towards evidence-based criticism, and a troubling “stable blindness” that sidelines the horse’s perspective. We conclude that meaningful reform requires (1) embedding validated behavioural and physical welfare indicators into all competition and pre-competition protocols, (2) transparent, evidence-inclusive rule-making under a precautionary principle, (3) genuine engagement with independent equine welfare experts, and (4) establishment of empowered, impartial oversight bodies to ensure that stated codes of conduct translate into consistent, enforceable practice. Only by catering to the horse’s subjective experiences and applying modern ethological and bioethical standards can equestrian sport retain its social licence and ensure integrity in all areas of competition management. Full article

Plasma amino acid (PAA) concentration in horses vary according to the exercise type. This study evaluated the changes in PAA levels and the associated metabolites, urea and ammonia, following short-duration, high-intensity cross-country exercise in eventing horses. Twenty eventing horses participated in 55 rides at 14 international competitions (2* to 4* levels) across five venues in Germany and Poland. Blood samples were collected at four timepoints: before exercise (TP0), at 10 min (TP1), and at 30 min (TP2) post-exercise, as well as in the morning on the day after the competition (TP3). A total of 23 different PAAs and two metabolites (ammonia and urea) were analyzed. PAA concentration difference over time was assessed by a mixed ANOVA. Significant fluctuations were observed in 18/25 parameters. For 21/23 PAAs, levels increased at TP1 and/or TP2, while cysteine concentrations decreased. Concentrations returned to pre-competition levels for 21/23 PAAs by TP3. Proline levels remained elevated (p = 0.002), while those of glycine significantly decreased (p = 0.027) at TP3. Plasma ammonia and urea levels increased at TP1, TP2 and TP3. This study provides foundations for supplementation strategies and can inform future works exploring PAAs’ role in performance and training adaptation in eventing horses and their potential as performance-related biomarkers. Full article

Breed bias exists within many of the top disciplines of equestrian sport. Therefore, the objective of this study was to evaluate the influence of breed on performance at the CCI5*-L level of eventing. To do so, the results of all CCI5*-L events from 2014 to 2024 were assessed. Initially, Thoroughbreds were compared to all non-Thoroughbreds, and this was followed by assessing the ten primary breeds. Data included penalties accrued in dressage, cross country, show jumping, and overall. Additionally, the likelihood of completing each phase was assessed. It was found that Thoroughbreds accrued more dressage penalties than non-Thoroughbreds (p < 0.001). When comparing primary breeds, Thoroughbreds were found to accumulate more dressage penalties than Hannoverian, Irish Sport Horse, Dutch Warmblood, Oldenburg, and Selle Francais (p < 0.03). When assessing cross-country, Thoroughbreds were more likely to finish without fault when compared to non-Thoroughbreds (p < 0.001). Non-Thoroughbreds were more likely to show jump without fault (p < 0.001), while Thoroughbred were found to have higher overall show jumping penalties in comparison to Anglo-European Studbook, Holsteiner, Irish Sport Horse, Dutch Warmblood, Oldenburg, and Selle Francais (p = 0.01). When evaluating overall penalties accumulated throughout the event, there was no effect of breed (p = 0.42). Overall, there was no advantage in competing a non-Thoroughbred in comparison to a Thoroughbred when considering total penalties accrued, indicating a level playing field within competition. Therefore, breed bias against TBs should be negated when selecting for CCI5*-L eventing prospects. Full article

Sport horses are frequently exposed to situations that were identified as stressors, indicated by an increased cortisol release, which might impair animal welfare. However, while many studies deal with the impact of exercise, transport, and competition on stress in horses, little is known about the early phase of a horse’s sports career and studies investigating the stress level of young horses during breaking-in are limited. To compare stress levels in unridden horses, horses during breaking-in, and horses in training we collected fecal samples of young, unridden horses (n = 28) and of horses in different training stages (n = 13) and measured fecal cortisol metabolite (FCM) concentrations. Our preliminary results showed that FCM concentrations of unridden horses were significantly lower than those of horses in training (Mann–Whitney rank sum test, p < 0.001). Particularly in the first year under the saddle FCMs were significantly higher than in unridden horses (one way ANOVA + post hoc Holm–Sidak test, p < 0.05), with a tendency for FCM levels to decrease with time in training. Furthermore, we observed that within the group of ridden horses there was a larger range of variability in FCM levels, suggesting individual variations regarding their ability to deal with (training-induced) stress. These results indicate that breaking-in is a stressful time for young horses, underlining the importance of carrying out the initial training as carefully as possible. Full article

Equine skin mycoses are a significant concern in Kazakhstan’s livestock industry due to the country’s historical livestock farming practices, the development of equestrian sports, and food traditions. Skin infections are among the most common fungal infections in horses. Emerging pathogens of equine dermatophytosis include keratinophilic Chrysosporium spp., which can degrade and metabolize keratin found in superficial tissues. This, combined with their thermotolerance, contributes to their pathogenicity. In this study, we investigated the biological properties and pathogenicity of two Chrysosporium strains isolated from equine skin lesions in northern and central Kazakhstan. Our findings showed that the Chrysosporium isolates caused a variety of clinically expressed skin lesions and exhibited cultural and morphological similarities to Trichophyton mentagrophytes. Genetic identification using ribosomal gene sequencing revealed 98.9% identity with Chrysosporium kreiselii and Chrysosporium zonatum sequences in both cases. The C. kreiselii strain caused pronounced skin lesions typical of classic dermatomycoses, demonstrated both keratinophilic and keratinolytic properties, and showed resistance to antifungal drugs. In contrast, the C. zonatum strain, which caused atypical lesions such as dandruff and seborrhea, was more sensitive to antifungal agents and exhibited keratinophilic properties. Our results highlight the emergence of new pathogenic Chrysosporium strains responsible for skin pathology in horses in Kazakhstan. We recommend that the identification of Chrysosporium skin infections in horses in Kazakhstan be followed by a comprehensive retrospective analysis of newly identified pathogens, including a full characterization of their pathogenicity. Full article

Information describing forces applied to the horse are needed to inform regulatory decisions regarding equine health and wellbeing. This study compares forces exerted beneath the noseband and headpiece of a snaffle bridle (SB) and a double bridle (DB). Horses were fitted with the same type of SB and DB. Forces were measured by pressure mats under the noseband (nasal/mandibular) and headpiece (occipital) of the bridle and by force sensors inserted bilaterally between the bit(s) and reins. The noseband was adjusted to 2 finger-equivalents using a tightness gauge. Data were recorded for eleven high-level dressage horses ridden in SB and DB in random order at collected walk, trot (sitting), and canter. The noseband pressures were similar between bridle types. Minimal, maximal, and mean occipital force and pressure were significantly higher for DB at walk, trot, and canter (all p ≤ 0.01), except minimal force for collected canter (p = 0.04). The rein tension for the bridoon bit alone and for the combined bridoon and curb bits was significantly lower than for the snaffle bit. Similar forces occur when ridden in SB and DB except that occipital force and pressure are higher due to the greater weight of the DB, and rein tension is lower for the DB. Full article

Preventing the spread of infectious diseases in horses requires breaking the chain of infection through appropriate prophylaxis. In Poland, where the horse population reached 273,006 in 2023, vaccinations are voluntary and primarily the responsibility of horse owners, with mandatory influenza vaccinations limited to sport horses. This study aimed to evaluate the vaccination status of Polish horses against infectious diseases through a survey of 980 horse owners and an analysis of 123 horse passports from slaughterhouses. Survey participants represented all provinces, predominantly owning warmblood horses (86%), but also 10% coldblood and 4% ponies or unidentified. Nearly half engaged in equestrian competitions. While over 90% of sport and pleasure horses were vaccinated against key diseases such as equine influenza and tetanus, only 2.4% of slaughter horses had similar prophylaxis. Cold-blooded horses were underrepresented in the survey (10.4%) but made up 68.3% of the analyzed slaughterhouse sample. The findings highlight a significant disparity in vaccination practices, primarily influenced by the horse’s intended use, with sport and leisure horses receiving better preventive care than slaughter horses. These results underscore the need for targeted educational efforts and policy interventions to improve the epizootiological status of Poland’s horse population and ensure a more uniform approach to disease prevention. A limitation of this study is the accuracy of vaccination records in horse passports, as some owners may not have them during vaccination, leading to discrepancies. Full article

Inappropriate, excessive, or overly strenuous training of sport horses can result in long-term injury, including the premature cessation of a horse’s sporting career. As a countermeasure, this study demonstrates the easy implementation of a biomechanical load monitoring system consisting of five commercial, multi-purpose inertial sensor units non-invasively attached to the horse’s distal limbs and trunk. From the data obtained, specific parameters for evaluating gait and limb loads are derived, providing the basis for objective exercise load management and successful injury prevention. Applied under routine in-the-field training conditions, our pilot study results show that tri-axial peak impact limb load increases progressively from walk to trot to canter, in analogy to stride frequency. While stance and swing phases shorten systematically with increasing riding speed across subjects, longitudinal and lateral load asymmetry are affected by gait at an individual level, revealing considerable variability between and within individual horses. This individualized, everyday approach facilitates gaining valuable insights into specific training effects and responses to changing environmental factors in competitive sport horses. It promises to be of great value in optimizing exercise management in equestrian sports to benefit animal welfare and long-term health in the future. Full article