Search Results (1,327)

Ragweed (Ambrosia artemisiifolia) represents a major and expanding source of aeroallergen exposure in Europe, with rising sensitization rates and substantial clinical impact. However, population-level data integrating airborne pollen exposure with detailed clinical sensitization patterns remain limited. We conducted a 5-year retrospective cross-sectional observational analysis of sensitization prevalence, complemented by a parallel temporal analysis of aerobiological data and symptom-driven healthcare presentation patterns (2020–2024) in Timisoara, Romania, including all patients undergoing first-time sensitization evaluation at a tertiary referral hospital. Sensitization was assessed using standardized skin prick testing to common aeroallergens and other allergen categories, while airborne ragweed pollen concentrations were monitored through a peri-urban network of real-time bio-particle analyzers. Statistical analyses included descriptive statistics, multivariable logistic regression, χ² tests for co-sensitization patterns, and comparative analyses of clinical manifestations across sensitization profiles. Among 4404 eligible patients, 50.7% were sensitized to at least one allergen. Ragweed sensitization was identified in 24.1% of patients, with a mean age of 31.1 years at diagnosis and no significant sex-related difference. Most ragweed-sensitized patients were polysensitized (71.5%), predominantly to other aeroallergens. Increasing age was independently associated with lower odds of polysensitization to other aeroallergens (adjusted OR = 0.97 per year, 95% CI: 0.96–0.98), while sex showed no independent association. Patients with ragweed sensitization alone and those cosensitized with aeroallergens exhibited similar prevalence of respiratory manifestations, whereas individuals with additional non-aeroallergen sensitization showed lower prevalence of rhinitis, conjunctivitis, and asthma but slightly higher rates of asthma exacerbations. Weekly diagnoses of ragweed sensitization demonstrated a pronounced seasonal peak between calendar weeks 33 and 38 (mid-August to late September), coinciding with peak airborne ragweed pollen concentrations. Ragweed sensitization therefore represents a substantial and seasonally driven healthcare burden in western Romania, characterized by frequent polysensitization, distinct clinical manifestation patterns across sensitization profiles, and close temporal alignment between airborne pollen exposure and clinical presentation. Integrating aerobiological monitoring with clinical surveillance may support targeted prevention strategies and improved patient management. Full article

Background/Objectives: The gut–brain axis is increasingly recognized as a critical modulator of cognitive function. This study investigated the neurotoxic effects of combined exposure to bacterial lipopolysaccharide (LPS) and the antiretroviral drug zidovudine (ZDV) in a mouse model, and evaluated the protective potential of two probiotic interventions: Bacillus subtilis and a mixture of lactobacilli. Methods: Cognitive function was assessed using the Morris water maze (MWM). Gut microbiota composition was analyzed by 16S rRNA sequencing, and intestinal morphology was examined histologically. Gene expression of neuroinflammatory markers and mitophagy-related genes in brain tissue was quantified by RT-PCR. Plasma levels of cell-free mitochondrial DNA (cf-mtDNA) were measured as a marker of mitochondrial damage. Results: Combined LPS + ZDV exposure induced systemic inflammation, impaired spatial memory, damaged the intestinal mucosa, and caused dysbiosis characterized by an increase in pro-inflammatory Muribaculaceae. In the brain, LPS + ZDV significantly upregulated Tnfa expression, confirming neuroinflammation. Bacillus subtilis administration prevented cognitive deficits, maintained Tnfa at control levels, and significantly reduced Il1b and Il6 expression compared to the LPS + ZDV group. This was accompanied by activation of the PINK1/PTEN-dependent mitophagy pathway, prevention of cf-mtDNA release, and restoration of gut microbial diversity. In contrast, the Lactobacilli mixture not only failed to improve outcomes but was associated with exacerbated intestinal damage, more pronounced cognitive dysfunction, and no reduction in neuroinflammatory markers. Conclusions: Combined exposure to LPS and ZDV induces gut–brain axis dysfunction characterized by neuroinflammation, cognitive impairment, intestinal damage, and dysbiosis. Bacillus subtilis effectively preserves cognitive function through activation of PINK1/PTEN-dependent mitophagy and suppression of neuroinflammation, highlighting its potential as a therapeutic candidate for cognitive impairments associated with gut–brain axis dysfunction. The contrasting effects of the lactobacilli mixture underscore the critical importance of strain-specificity in probiotic interventions. Full article

Neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis, currently represent one of the major challenges in contemporary medicine and geriatrics. Progressive degeneration of the nervous system affects not only patients’ physical functioning but also their psychosocial well-being, often leading to social isolation and disruption of interpersonal relationships. These processes are most strongly associated with individuals over 65 years of age, in whom metabolic syndrome is frequently diagnosed and constitutes a significant factor predisposing them to the exacerbation of neuropathological changes. This review analyzes the role of selected vitamins in modulating the course of neurodegenerative disorders, with particular emphasis on their neuroprotective potential. Specific attention is given to their involvement in antioxidant defense mechanisms, regulation of inflammatory pathways, prevention of abnormal protein aggregation, participation in neurotransmitter synthesis, and support of mitochondrial function and cellular energy metabolism. The review also considers key interactions between vitamins and coenzyme Q10, which synergistically enhance neuroprotective mechanisms. Deficiencies in certain vitamins may exacerbate oxidative stress, impair synaptic transmission, and intensify neuroinflammatory responses, thereby contributing to disease progression. The study analyzes the available data on therapeutic doses of vitamins and compares them with the recommended dietary intake and the upper tolerable intake levels (UL). The available evidence suggests that personalized vitamin supplementation, when integrated with a well-balanced and nutrient-dense diet, may constitute a valuable adjunctive therapeutic strategy. Such an approach may help attenuate disease progression, support neuronal integrity, and improve functional outcomes. Ultimately, targeted nutritional interventions may enhance overall well-being and quality of life in patients affected by neurodegenerative diseases. Full article

Standing tile inlets are commonly used to drain unwanted surface water from croplands but can exacerbate pollution by facilitating the transport of nutrients to waterways. Blind inlets have increasingly been viewed as a beneficial alternative to standing inlets since they control erosion and capture particulate nutrients. However, conventional blind inlets do little to limit dissolved nutrient transport, and modified blind inlet (MBI) designs have been proposed that incorporate woodchips—a medium that facilitates denitrification. While initial investigations have highlighted MBIs’ remediation potential, their ability to meet prescribed drainage standards has not been well-documented. In this study, we designed and installed MBIs composed of pea gravel and woodchips in two eastern Iowa fields under row crop cultivation. Flow and nitrate were continuously monitored using in situ equipment directly downstream of the MBIs (February 2023–June 2025). Observed flows were very ephemeral, consisting of ~25 distinct events at both sites, with no flow recorded in between. During several wet weather events, flow rates exceeded the MBIs’ design requirements, confirming their sufficient drainage capacity to prevent in-field ponding. Nitrate concentrations varied considerably, with long-term averages of 11.6 and 19.1 mg/L and overall loadings of 4.94 and 7.10 kg during our 28-month study. We also measured phosphate and sulfate during select wet weather events, and discrepancies in concentrations between inlets and outlets suggested that groundwater was often present alongside surficial drainage in our monitoring setup. We believe our results argue for increased adoption of MBIs in conservation and further quantification of their remediation capabilities. Full article

The emotional responses to bullying victimization are central to its impact on subsequent psychological consequences, but the role of specific emotions is insufficiently defined within a comprehensive framework. In order to enhance the understanding of the emotional experiences of bullying victims, the present review examines the role of self-conscious emotions (i.e., humiliation, shame, and guilt), the role of basic emotions (i.e., anger and fear), and various psychological consequences (e.g., anxiety, depression, suicidal ideation). A non-systematic, narrative approach was employed to synthesize the findings, with a total of 343 articles included in the review. Self-conscious emotions appear to be central to bullying victimization, with humiliation being particularly pivotal due to its link to internalized self-devaluation, perceived injustice, and attribution of cruelty to the perpetrator. In turn, anger and fear seem to constitute crucial basic emotions in response to bullying dynamics. Although anger may escalate aggression, it may also facilitate positive confrontational behaviors when properly channeled, whereas fear may contribute to avoidance and increased victimization if sustained. Adverse psychological consequences such as anxiety, depression, stress, low self-esteem, and suicidal ideation are prevalent among victims of bullying, potentially exacerbated in vulnerable groups. Future research should further explore the role of emotions in the context of bullying victimization, examining their impact on both mental health outcomes and behavioral patterns over time. Exploring how different emotional responses interact and influence each other within bullying dynamics could provide insights into effective intervention strategies, and a more comprehensive understanding of the sociocultural factors influencing emotional responses to bullying might help in customizing prevention and support measures across diverse contexts. Full article

Deleterious crosstalk between the gut and distant organs is a key factor behind disease progression. Currently, the molecular signals mediating this communication remain elusive. We hypothesized that systemic oxidative stress and oxidatively modified serum proteins transmit injury signals from extraintestinal sites to the gut. In various murine models of organ injury, primary damage was consistently associated with systemic oxidative stress and intestinal damage. Specifically, ischemia/reperfusion (I/R)-induced acute kidney injury caused profound colonic barrier defects. Depleting the microbiota with antibiotics markedly improved survival and attenuated both renal and colonic injury, implicating translocated microbes in exacerbating pathology. Mechanistically, these changes were linked to systemic oxidative stress and were largely prevented by the antioxidant N-acetylcysteine. Furthermore, serum from I/R mice disrupted epithelial barrier integrity and induced cell death in vitro, effects that were recapitulated by exposure to oxidized serum proteins. Characterization of serum components identified albumin as the predominantly oxidized protein, which displayed potent cytotoxicity toward cultured intestinal epithelial cells. Our findings establish oxidative stress and oxidized serum albumin as key pathogenic factors mediating the detrimental interaction between remote organs and the gut. These data suggest that targeting oxidative modifications offers a promising therapeutic strategy to disrupt this pathological loop in critical illness. Full article

The acceleration of climate change poses a growing threat to human health, particularly by exacerbating non-communicable diseases (NCDs) such as cardiovascular and respiratory conditions. Rising global temperatures amplify air pollution and environmental toxins, disproportionately affecting vulnerable populations. This narrative review explores the complex pathways linking climate-related environmental stressors to adverse health outcomes, focusing on the intermediary roles of epigenetic modifications and alterations in the microbiota. Epigenetic processes, including DNA methylation and histone modifications, may mediate how environmental exposures influence gene expression and disease susceptibility. Concurrently, changes in microbiota composition induced by pollutants and temperature fluctuations can promote inflammatory responses and immune dysfunction. Elucidating these molecular mechanisms is essential for developing targeted interventions and adaptive strategies to mitigate the health impacts of climate change. This review underscores the importance of identifying epigenetic and microbiota-based biomarkers for early risk stratification and for informing public health prevention and adaptation policies. A transdisciplinary approach, grounded in the One Health framework, is critical to addressing the growing burden of climate-sensitive diseases and reducing health inequalities. Full article

Cholestasis in children is characterized by impaired bile flow that disrupts hepatic metabolism, nutrient homeostasis, and effects trace element balance. This narrative review summarizes current evidence on the metabolism, biological functions, and clinical implications of key trace elements—zinc, selenium, copper, and manganese—in pediatric cholestatic liver disease. The liver regulates trace element absorption, intracellular trafficking, storage, and biliary excretion; cholestasis alters these processes, leading to deficiencies or toxic accumulation. Zinc and selenium deficiencies are common and contribute to impaired growth, immune dysfunction, oxidative stress, and delayed hepatic regeneration. Conversely, reduced biliary excretion promotes copper and manganese accumulation, potentially exacerbating liver injury and causing manganese-related neurotoxicity. Recent advances in understanding metal-specific hepatic transporters and trafficking pathways have provided mechanistic insight into these alterations. Management strategies emphasize individualized supplementation, monitoring during enteral and parenteral nutrition, and prevention of deficiency and toxicity. Precision-based nutritional approaches may improve outcomes in pediatric cholestatic liver disease. Full article

Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder. The nutritional status of vitamin D, an essential micronutrient, is closely linked to the onset and progression of T2DM. A growing body of research has shown that gut microbiota and its metabolites are emerging as a biological link connecting vitamin D and systemic glucose metabolism. Gut dysbiosis is prevalent in T2DM patients, which is characterized by reduced gut microbial diversity, increased abundance of pathogenic bacteria, and abnormal production of key metabolites such as short-chain fatty acids, bile acids and tryptophan derivatives. These abnormal changes in gut microecology and metabolites can impair intestinal barrier integrity and induce chronic low-grade inflammation in the body, and vitamin D deficiency may further exacerbate these abnormal processes. The evidence suggests that the regulatory effect of vitamin D on systemic glucose metabolism may be partially achieved through gut microbiota-related pathways. This review aims to explore whether, and by what mechanisms, the gut microbiota mediates the regulatory effect of vitamin D on T2DM. It also intends to conduct an analysis of the potential molecular mechanisms underlying the interactions between vitamin D, gut microbiota and T2DM, so as to provide a new theoretical basis and research ideas for the prevention and intervention of T2DM. Full article

Both gut microbiota dysbiosis and disrupted cholesterol metabolism are associated with colorectal cancer (CRC). While the interactions between these two factors have been well explored in diseases such as cardiovascular disease and atherosclerosis, their interactions and underlying mechanisms in CRC pathogenesis remain insufficiently explored, constituting a critical area for further investigation. This review examines the complex relationship between gut microbiota and cholesterol metabolism in CRC development from 2 perspectives: how specific gut microbial species can increase CRC risk by modulating cholesterol metabolism, particularly through bile acids and oxysterols, and how disrupted cholesterol metabolism can exacerbate microbial dysbiosis and promote CRC. The bidirectional relationship between gut dysbiosis and cholesterol dysregulation creates a vicious cycle that drives CRC development. Moreover, the potential of targeting the gut microbiome and cholesterol metabolism to develop new strategies for preventing and treating CRC is discussed, highlighting the promise of certain bacterial strains that exert protective effects via cholesterol-lowering mechanisms. By elucidating the intricate connections between gut microbiota, cholesterol metabolism, and CRC, this review paves the way for innovative approaches in CRC prevention and therapy. Full article

To address the engineering challenges of frequent flexural deformation and instability of composite roadway roofs and the difficulty in accurately controlling the support strength range during deep coal mining, this study takes the soft–hard interbedded composite roof of the working face in the West No. 1 Mining Area of Shuangyang Coal Mine in Shuangyashan as the engineering background. Typical fine sandstone (hard rock) and tuff (soft rock) from the on-site roof were selected to prepare layered composite specimens, and indoor four-point bending tests were conducted. Combined with theoretical calculations, strain monitoring, and acoustic emission (AE) real-time localization technology, the regulatory mechanisms of three key factors—lithological combination, loading rate, and span—on the flexural mechanical properties, deformation and failure modes, and fracture evolution laws of layered composite rock masses were systematically investigated. The research results show the following: (1) The flexural performance of layered composite rock masses is dominated by the interlayer interface effect. Their flexural strength is 46.7% and 41.1% lower than that of single hard rock and soft rock specimens, respectively, and the competitive mechanism between interface slip and delamination fracture is the core inducement of strength deterioration. (2) The strength and deformation characteristics of layered composite rock masses exhibit a significant loading rate effect. When the loading rate increases from 0.002 mm/s to 0.02 mm/s, the flexural strength decreases by 51.8% and the mid-span deformation deflection reduces by 50.1%. High loading rates will exacerbate the deformation mismatch between soft and hard rock layers, trigger premature failure of interface bonding, and inhibit the full development of structural plastic deformation. (3) Increasing the span significantly optimizes the flexural bearing performance of layered composite rock masses. When the span increases from 170 mm to 190 mm, the flexural strength increases by 65.7% and the mid-span deformation deflection synchronously increases by 65.7%. A large span can extend the flexural deformation path, promote the coordinated deformation of rock layers, and suppress local stress concentration. (4) The flexural failure of layered composite rock masses is dominated by Mode II shear cracks, while single-lithology specimens are mainly dominated by Mode I tensile cracks. Loading rate and span significantly change the crack propagation mode and energy release law. This study establishes a calculation method for the equivalent flexural stiffness of layered composite rock masses and reveals the mesoscopic mechanism of flexural failure of heterogeneous layered rock masses. The research results can provide a theoretical basis and experimental support for the optimization of support schemes and the prevention and control of roof collapse hazards for composite roofs of deep coal mine roadways. Full article

Cold exposure may influence reproductive health through vascular changes, yet its mechanisms remain underexplored. This study aimed to investigate the impact of cold exposure on uterine blood vessels and the expression of the AMPK/PGC-1α gene and protein in adult female SD rats. A primary dysmenorrhea model was established in female Sprague Dawley rats and subjected to continuous cold exposure. Changes in body weight, ear temperature, and estrous cycle were observed. Superoxide dismutase (SOD) activity and adenosine triphosphate (ATP) levels were measured to assess oxidative stress. Uterine tissue morphology was assessed via small animal ultrasound, microcirculation observed using RFLSI imaging, and vascular morphology along with caspase-3 and AMPK expression evaluated histologically and immunohistochemically. CD31 and TUNEL double immunofluorescence were used to assess vascular endothelial apoptosis levels. Western blot was used to analyze Bax, BCL-2, and pAMPK/AMPK expression levels. In vitro injury models were used to treat human umbilical vein endothelial cells (HUVECs) with cold stimulus using the AMPK inhibitor Compound C. RT-PCR quantified Bax, AMPK, p53, and PGC-1α expression. Hypothermia-exposed rats exhibited significantly reduced body weight and ear temperature (p < 0.05), prolonged estrous cycle (p < 0.01), and decreased uterine index (p < 0.01), accompanied by reduced SOD and ATP levels (p < 0.01, p < 0.05). Ultrasound and flow imaging revealed decreased uterine blood flow velocity in the hypothermia group (p < 0.01). Histomorphology revealed disorganized uterine cell arrangement, reduced uterine vessel count (p < 0.01), and increased mean vessel area (p < 0.01) in cold-exposed uteri. Immunofluorescence detection revealed increased vascular endothelial cell apoptosis (p < 0.05). Western blot results showed that proapoptotic protein Bax was upregulated (p < 0.01), Bcl-2 was downregulated (p < 0.05), p-AMPK and p-AMPK/AMPK ratio were elevated (p < 0.01) after cold exposure; Rt-qPCR results indicated that Bax and P53 mRNA were increased (p < 0.01), while PGC-1α expression was elevated (p < 0.01). Rt-qPCR results showed elevated Bax and p53 mRNA (p < 0.01), along with increased AMPK and PGC-1α expression (p < 0.01) in the cold-exposed group. In human umbilical vein endothelial cells (HUVECs), compound C attenuated cold-induced effects (p < 0.01) and downregulated Bax and AMPK expression (p < 0.01). Cold exposure exacerbates uterine oxidative stress and energy imbalance, disrupts microcirculatory homeostasis, and induces endothelial cell apoptosis. Excessive phosphorylation of AMPK may co-activate PGC-1α, jointly contributing to cold-induced uterine dysfunction and exacerbated dysmenorrhea. This study reveals potential signaling pathways underlying cold-induced uterine vascular abnormalities, providing novel theoretical foundations and targeted intervention strategies for the prevention and treatment of primary dysmenorrhea. Full article

Identifying environmental factors contributing to allergic asthma is critical for effective prevention. PM_2.5, a major environmental pollutant, and high relative humidity frequently coexist in urban and industrialized regions, particularly when ventilation is poor. However, the combined effects of PM_2.5 and humidity remain unclear. This study used a murine asthma model, exposing male Balb/c mice sensitized with ovalbumin (OVA) to PM_2.5 (75 μg/m³ and 35 μg/m³), based on China’s Ambient Air Quality Standards (GB3095-2012), and/or varying relative humidity levels in a controlled chamber. Allergic asthma severity was evaluated through histopathological changes, pulmonary function, Th1/Th2 balance, mucus hypersecretion, and inflammatory factor levels, alongside the activation of TRPV4 and MAPK signaling pathways (ERK, p38MAPK, and JNK). The results showed that high humidity (90%) or PM_2.5 exposure alone had minimal impact, but combined exposure to 75 μg/m³ PM_2.5 and 90% humidity markedly aggravated airway hyperresponsiveness, inflammation, and mucus hypersecretion. These changes coincided with enhanced TRPV4 activation and MAPK signaling, particularly p38MAPK and JNK, while ERK1/2 remained unaffected. A lower PM_2.5 concentration (35 μg/m³) combined with 90% humidity had a weaker impact. Blocking TRPV4 with HC-067047 effectively mitigated asthma exacerbation caused by combined exposure. These findings demonstrate that co-exposure to PM_2.5 and high humidity dose-dependently exacerbates allergic asthma, an effect likely mediated by TRPV4-MAPK pathway activation. Targeting TRPV4 may offer a potential therapeutic strategy to mitigate asthma exacerbation in environments with high humidity and PM_2.5. Full article

Intensive Care Unit Acquired Weakness (ICUAW) is a highly prevalent neuromuscular complication affecting around 40% of critically ill patients, rising to over 80% in high-risk cohorts. It is independently associated with prolonged mechanical ventilation, increased intensive care unit (ICU) and hospital length of stay, elevated mortality (in-hospital, 1-year, and 5-year), higher healthcare costs, and long-term functional impairment. ICUAW is clinically defined by symmetric flaccid tetraparesis, frequently involving respiratory muscles, and exhibits significant pathobiological heterogeneity. Further subclassification is based on neurotopographic patterns: Critical Illness Polyneuropathy (CIP), Myopathy (CIM), and Polyneuromyopathy (CIPNM). Diagnosis typically relies on the Medical Research Council (MRC) Sum Score, with a threshold of <48 indicating clinically relevant weakness. While adjunct modalities such as electromyography/nerve conduction studies support assessment, their utility may be limited by patient cooperation and availability. Preventive strategies center on modifiable metabolic factors. Caloric and protein deficits exacerbate catabolism, while overfeeding—linked to anabolic resistance and stress hyperglycemia—also impairs recovery. To date, pharmacologic interventions remain inconclusive. However, early mobilization and neuromuscular electrical stimulation are promising non-pharmacologic strategies. The multifactorial and heterogeneous pathophysiology of ICUAW highlights the need for a biologically refined definition that can guide future targeted therapeutic interventions. Comprehensive multimodal strategies, together with structured long-term follow-up in Post-Intensive Care Syndrome (PICS) clinics, are essential for improving outcomes in this prevalent complication of critical care. Full article

Quadruped robots possess strong adaptability to rugged terrain, soft ground, and multi-obstacle environments, offering broad application prospects in extraterrestrial planetary exploration. However, large diurnal temperature variations on extraterrestrial bodies exacerbate joint friction nonlinearity, degrading motion control accuracy and stability. To address this, a quadruped robot prototype with hybrid serial–parallel legs is designed for lunar exploration, and an 18-DOF dynamic model is derived using d’Alembert’s principle. Based on the PPO (Proximal Policy Optimization) reinforcement learning algorithm, joint friction parameters are identified using joint velocity and foot–ground contact force. By introducing friction compensation and contact force, an accurate dynamics-based feedback linearization control model is constructed, and a motion impedance control law is designed. Finally, joint friction parameters are identified and validated through both virtual and experimental prototypes, and the proposed control method is tested on flat and sloped terrain. Results show that the method can precisely regulate contact force and foot position, keeping RMSE (Root Mean Square Error) of position within 21.04 mm while preventing slipping and false contact. Full article