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15 pages, 2651 KB  
Review
Application of Regenerative Agriculture: A Review and Case Study in an Agrosilvopastoral Region
by Raimundo Jiménez-Ballesta, Jorge Mongil-Manso and Adrián Jiménez-Sánchez
Sustainability 2025, 17(20), 9066; https://doi.org/10.3390/su17209066 (registering DOI) - 13 Oct 2025
Abstract
While agriculture is experiencing localized crises, its indispensable role as the foundation of humanity’s food supply requires its uninterrupted functioning. This conventional system is therefore in a state of competition with alternative models, particularly agroecology, which offers a different paradigm for food production. [...] Read more.
While agriculture is experiencing localized crises, its indispensable role as the foundation of humanity’s food supply requires its uninterrupted functioning. This conventional system is therefore in a state of competition with alternative models, particularly agroecology, which offers a different paradigm for food production. Given this situation and the need to gather reliable information on regenerative agriculture (RA), this article provides a literature review on its principles, objectives, and edaphic benefits. Additionally, it presents a case study that offers practical knowledge of the techniques and actions implemented by an agroforestry farm in central Spain. With this goal, this article addresses key aspects of RA, such as the use of cover crops, and the integration of livestock, emphasizing its role in improving soil quality and increasing biodiversity, among other benefits. After reviewing numerous scientific articles, and despite widespread interest in RA, there is no commonly accepted definition, so there is a wide range of ways to define RA. Until a generalized definition is accepted, we advocate making proposals and implementing methods with extreme caution and based on the regional or local context in which it is defined. In this sense, based on the implementation of RA at the Kerbest Foundation farm, we propose regenerative agriculture as a set of agroecological actions and processes that fundamentally provide functional soil quality, food quality, ecosystem services, and, especially, healthy and economically profitable livestock farming. Based on all of the above, we can argue that RA is no longer merely a commitment made by farmers but, rather, an environmentally, economically, and socially sustainable solution grounded in scientific knowledge and technical experience. Full article
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19 pages, 562 KB  
Review
A Review on the Adoption of Sustainable Agricultural Practices in Southern Africa: Focus on Smallholder Farmers
by Jonathan Thobane, Jorine Ndoro, Solly Molepo, Batizi Serote, Samkelisiwe Hlophe-Ginindza, Sylvester Mpandeli, Luxon Nhamo and Salmina Mokgehle
Agriculture 2025, 15(20), 2125; https://doi.org/10.3390/agriculture15202125 - 13 Oct 2025
Abstract
Food insecurity, financial loss, and a decline in agricultural output are among the significant challenges to the global food chain caused by extreme climatic events, high variability and change, rapid urbanization, and land degradation. Therefore, it is essential to explore alternative, sustainable agricultural [...] Read more.
Food insecurity, financial loss, and a decline in agricultural output are among the significant challenges to the global food chain caused by extreme climatic events, high variability and change, rapid urbanization, and land degradation. Therefore, it is essential to explore alternative, sustainable agricultural practices to meet the growing population’s food needs. Sustainable agriculture is foundational to farm management, rural development, and water conservation. This includes sustainable practices such as crop rotation, intercropping, and planting crops with varying rooting depths to maximize soil moisture absorption, as well as mulching to improve nutrient recycling and enhance productivity in smallholder cropping systems. The adoption of sustainable agricultural practices has become a priority for smallholder farmers, policymakers, extension agents, and agricultural experts to improve agricultural productivity, contribute to food security, and generate income. However, adoption rates have been slow, especially in Southern Africa, due to a lack of access to technology, financial constraints, limited information, and limited knowledge. This review was conducted using a comprehensive literature search on the adoption of sustainable agricultural practices by legume smallholders, examining various factors that contribute to the failure of legume smallholder farmers to adopt new agricultural practices. The timeframe of the reviewed literature was from 2010 to 2024. The results showed that smallholder farmers face numerous challenges, including limited access to technology, inadequate knowledge, and insufficient financial resources. Research conducted by the Water Research Commission (WRC) indicates that commercial farmers have access to technology, and this group of farmers possesses more substantial financial resources compared to smallholder farmers. In the adoption of sustainable agricultural practices. It is essential to strengthen the linkage between researchers, agricultural extension, and legume smallholder farmers to promote sustainable agricultural practices (SAPs). Smallholder farmers must be informed about such interventions and sustainable agricultural practices to improve rural livelihoods and enhance resilience, adaptation, and responsiveness. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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22 pages, 6988 KB  
Article
Efficacy of Hybrid Photovoltaic–Thermal and Geothermal Heat Pump System for Greenhouse Climate Control
by Chung Geon Lee, Geum Choon Kang, Jae Kyung Jang, Sung-Wook Yun, Jong Pil Moon, Hong-Seok Mun and Eddiemar Baguio Lagua
Energies 2025, 18(20), 5386; https://doi.org/10.3390/en18205386 (registering DOI) - 13 Oct 2025
Abstract
This study evaluated the performance of a hybrid heat pump system integrating photovoltaic–thermal (PVT) panels with a standing column well (SCW) geothermal system in a strawberry greenhouse. The PVT panels, installed over 10% of the area of a 175 m3 greenhouse, stored [...] Read more.
This study evaluated the performance of a hybrid heat pump system integrating photovoltaic–thermal (PVT) panels with a standing column well (SCW) geothermal system in a strawberry greenhouse. The PVT panels, installed over 10% of the area of a 175 m3 greenhouse, stored excess solar heat in an aquifer to offset the reduced efficiency of the geothermal source during extended operation. The results showed that the hybrid system can supply 11,253 kWh of heat energy during the winter, maintaining the night time indoor temperature at 10 °C even when outdoor conditions dropped to −10.5 °C. The PVT system captured 11,125 kWh of solar heat during heating the off season, increasing the heat supply up to 22,378 kWh annually. Additionally, the system generated 3839 kWh of electricity, which significantly offset the 36.72% of the annual pump system electricity requirements, enhancing the system coefficient of performance (COP) of 3.38. Strawberry production increased by 4% with 78% heating cost saving compared to a kerosene boiler system. The results show that the PVT system effectively supports the geothermal system, improving heating performance and demonstrating the feasibility of hybrid renewable energy in smart farms to enhance efficiency, reduce fossil fuel use, and advance carbon neutrality. Full article
21 pages, 943 KB  
Article
Forecasting the Development of Offshore Wind Energy in Poland in the Context of the Energy Transformation and Sustainable Development Goals
by Aurelia Rybak, Aleksandra Rybak and Spas D. Kolev
Energies 2025, 18(20), 5380; https://doi.org/10.3390/en18205380 (registering DOI) - 13 Oct 2025
Abstract
This article presents the results of research on the potential development of offshore wind energy in Poland. Wind energy generated in offshore farms is intended to be the second pillar (alongside nuclear power) of Poland’s energy transition, creating the foundation for a zero-emission [...] Read more.
This article presents the results of research on the potential development of offshore wind energy in Poland. Wind energy generated in offshore farms is intended to be the second pillar (alongside nuclear power) of Poland’s energy transition, creating the foundation for a zero-emission energy system. The authors constructed a neural network that allowed them to forecast the development of the installed offshore energy capacity for Poland by 2030. For this purpose, the factors that have the greatest impact on the development of wind energy in Poland were identified. This knowledge will facilitate the development of state policy consistent with the Sustainable Development Goals (SDGs) and the European Green Deal. Since Poland currently does not have installed offshore wind energy capacity, Germany was used as a benchmark to train the model. The research results fill the identified gap: to date, forecasts of offshore development in Poland based on a model trained on German data have not been presented in the literature. The research results show that by 2030, Poland can achieve the goals set by the United Nations, the European Union, and the Polish Energy Policy 2040 (PEP2040). The PEP2040 assumes that Poland should have 5.9 GW of energy installed in offshore wind farms in the Baltic Sea by 2030. The forecast indicates that this will be approximately 5.3 GW, with the difference between these values remaining within the model’s margin of error. Full article
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17 pages, 8674 KB  
Article
A Study Toward More Ethical Commercial Aquaculture by Leveraging Rheotaxis
by Alex Raposo, Benjamin Reading, Mike Frinsko and David L. Roberts
Animals 2025, 15(20), 2961; https://doi.org/10.3390/ani15202961 (registering DOI) - 13 Oct 2025
Abstract
The welfare of farmed hybrid striped bass remains largely unaddressed in U.S. aquaculture, despite the species’ economic significance and the scale of production. Physical handling during grading and inspection not only causes stress and increased incidence of injury, but also results in unmarketable [...] Read more.
The welfare of farmed hybrid striped bass remains largely unaddressed in U.S. aquaculture, despite the species’ economic significance and the scale of production. Physical handling during grading and inspection not only causes stress and increased incidence of injury, but also results in unmarketable fish and significant financial loss for producers. To address these issues, we present a prototype system that uses directed water currents to leverage the fish’s natural rheotactic behavior, enabling directed movement between tank regions without the need for direct physical contact. Our design allows for early identification of malformed individuals, who otherwise face prolonged suffering and starvation, so they can be humanely culled. In a small pilot study, we observed that fish moved into the destination region more frequently and with less behavioral variability when exposed to a directed current, suggesting this method as a viable alternative to traditional handling. While the system requires further refinement and testing at scale, these preliminary results offer a promising step toward ethical, commercially viable, and low-stress fish sorting systems in commercial aquaculture. Full article
(This article belongs to the Special Issue Animal–Computer Interaction: New Horizons in Animal Welfare)
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20 pages, 1650 KB  
Article
Is Solar Panel Adoption a Win–Win Strategy for Chicken Farms? Evidence from Agriculture Census Data
by Tzong-Haw Lee, Yu-You Liou and Hung-Hao Chang
Agriculture 2025, 15(20), 2124; https://doi.org/10.3390/agriculture15202124 - 13 Oct 2025
Abstract
Concerns over ground-mounted photovoltaics (PVs) on cropland have encouraged a shift toward rooftop PV systems on livestock and poultry farms. Using ex-post observational data and a doubly robust estimation approach, this study examines the determinants and economic effects of PV adoption among chicken [...] Read more.
Concerns over ground-mounted photovoltaics (PVs) on cropland have encouraged a shift toward rooftop PV systems on livestock and poultry farms. Using ex-post observational data and a doubly robust estimation approach, this study examines the determinants and economic effects of PV adoption among chicken farmers in Taiwan. Based on a population-wide agricultural census, we assess how socio-demographic factors, production practices, household composition, and electricity infrastructure influence adoption decisions. The results show that education level, household structure, and access to electricity are key drivers of adoption. PV adopters exhibit a 5.8% higher sales value of chicken products, mainly due to increased production volume rather than quality improvements. These findings highlight the potential dual benefits of integrating solar energy with poultry farming and provide policy-relevant insights for sustainable agricultural development. Full article
(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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23 pages, 2205 KB  
Article
Evidence of Agroecological Performance in Production Systems Integrating Agroecology and Bioeconomy Actions Using TAPE in the Colombian Andean–Amazon Transition Zone
by Yerson D. Suárez-Córdoba, Jaime A. Barrera-García, Armando Sterling, Carlos H. Rodríguez-León and Pablo A. Tittonell
Sustainability 2025, 17(20), 9024; https://doi.org/10.3390/su17209024 (registering DOI) - 12 Oct 2025
Abstract
The expansion of conventional agricultural models in the Colombian Amazon has caused deforestation, biodiversity loss, and socio-environmental degradation. In response, agroecology and bioeconomy are emerging as key strategies to regenerate landscapes and foster sustainable production systems. We evaluated the agroecological performance of 25 [...] Read more.
The expansion of conventional agricultural models in the Colombian Amazon has caused deforestation, biodiversity loss, and socio-environmental degradation. In response, agroecology and bioeconomy are emerging as key strategies to regenerate landscapes and foster sustainable production systems. We evaluated the agroecological performance of 25 farms in the Andean–Amazon transition zone of Colombia using FAO’s Tool for Agroecology Performance Evaluation (TAPE). The analysis included land cover dynamics (2002–2024), characterization of the agroecological transition based on the 10 Elements of Agroecology, and 23 economic, environmental, and social indicators. Four farm typologies were identified; among them, Mixed Family Farms (MFF) achieved the highest transition score (CAET = 60.5%) and excelled in crop diversity (64%), soil health (SHI = 4.24), productive autonomy (VA/GVP = 0.69), and household empowerment (FMEF= 85%). Correlation analyses showed strong links between agroecological practices, economic efficiency, and social cohesion. Land cover dynamics revealed a continuous decline in forest cover (12.9% in 2002 to 7.1% in 2024) and an increase in secondary vegetation, underscoring the urgent need for restorative approaches. Overall, farms further along the agroecological transition were more productive, autonomous, and socially cohesive, strengthening territorial resilience. The application of TAPE proved robust multidimensional evidence to support agroecological monitoring and decision-making, with direct implications for land use planning, rural development strategies, and sustainability policies in the Amazon. At the same time, its sensitivity to high baseline biodiversity and to the complex socio-ecological dynamics of the Colombian Amazon underscores the need to refine the methodology in future applications. By addressing these challenges, the study contributes to the broader international debate on agroecological transitions, offering insights relevant for other tropical frontiers and biodiversity-rich regions facing similar pressures. Full article
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13 pages, 8068 KB  
Article
Application of Water-Sensitive Paper for Spray Performance Evaluation in Aeroponics via a Segmentation-Based Algorithm
by Muhammad Amjad, Yeong-Hyeon Shin, Je-Min Park, Woo-Jae Cho and Uk-Hyeon Yeo
Appl. Sci. 2025, 15(20), 10928; https://doi.org/10.3390/app152010928 - 11 Oct 2025
Viewed by 41
Abstract
Continued population growth demands a significant increase in agricultural production to ensure food security. However, agricultural output is limited by environmental crises and the negative impacts of open-field farm practices. As an alternative, vertical farming techniques, such as aeroponics, can be utilized to [...] Read more.
Continued population growth demands a significant increase in agricultural production to ensure food security. However, agricultural output is limited by environmental crises and the negative impacts of open-field farm practices. As an alternative, vertical farming techniques, such as aeroponics, can be utilized to optimize the use of resources. However, the uneven size and distribution of spray droplets in aeroponics, issues that affect root development and nutrient delivery, continue to be problematic in spray performance analysis. In aeroponics, nutrient solutions are delivered to plant roots through pressurized nozzles, and the effectiveness of this delivery depends on the spray characteristics. Variations in flow rates directly affect droplet size, density, and coverage, which in turn influence nutrient uptake and crop growth. In this study, the flow rate was adjusted (3, 4.5, and 6 L/min) to quantitatively analyze spray performance using water-sensitive paper (WSP) as a deposit collector via a quick assessment method. Subsequently, image-processing techniques such as threshold segmentation and morphological operations were applied to isolate individual spray droplets on the WSP images. This technique enabled the quantification of the droplet’s coverage area, size, density, and uniformity to effectively evaluate spray performance. One-way ANOVA indicated that all the spray parameters varied significantly with respect to the flow rate (p < 0.05): For example, the average diameters of the droplets increased from 0.73 mm at 3 L/min to 1.29 mm at 6 L/min. The droplets’ densities decreased from 85.53 drops/cm2 to 30.00 drops/cm2 across the same flow range. The average uniformity index improved from 30.53 to 15.95 as the flow rate increased. These results indicate that the application of WSP is an effective and scalable approach for analyzing spray performance in aeroponics, as WSP can be rapidly digitized with simple tools, such as a cell phone camera, avoiding the limitations of flatbed scanners or specialized imaging systems. Full article
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30 pages, 2049 KB  
Article
From Sea to Table: The Role of Traceability in Italian Seafood Consumption
by Simona Violino, Damianos Chatzievangelou, Giulio Sperandio, Simone Gaetano Amato, Chiara Fini, Domenico Ciorciaro, Simone Figorilli, Cecilia Ripa, Simone Vasta, Francesca Antonucci, Federico Pallottino, Raffaele De Luca, Daniela Scutaru, Sara Biancardi, Elisa Pignoni and Corrado Costa
Foods 2025, 14(20), 3469; https://doi.org/10.3390/foods14203469 (registering DOI) - 11 Oct 2025
Viewed by 54
Abstract
Seafood plays a key role in a healthy diet due to its high content of essential nutrients. However, its global trade and complex supply chains expose it to frequent mislabeling and food fraud. This study investigates Italian consumers’ willingness to pay (WTP) for [...] Read more.
Seafood plays a key role in a healthy diet due to its high content of essential nutrients. However, its global trade and complex supply chains expose it to frequent mislabeling and food fraud. This study investigates Italian consumers’ willingness to pay (WTP) for traceable seafood products, exploring how blockchain technology (BT) can enhance transparency and economic sustainability in the fish supply chain. An online questionnaire, administered in 2022 and 2024, gathered responses from a diverse demographic, focusing on four representative seafood species: farmed sea bass, striped venus clams, giant red shrimp, and albacore tuna. Results indicate that most respondents—primarily with higher education levels—value traceability and are willing to pay a premium for certified, traceable seafood. The study models the economic feasibility of implementing BT at both national and regional levels and finds that the consumer’s WTP exceeds the additional costs incurred by adopting BT. These findings support the viability of traceability systems in improving food safety and sustainability, while reinforcing consumer trust. The results also underscore the importance of providing clear information at the point of purchase, particularly regarding species, origin, and production methods—factors critical to informed seafood choices and advancing more sustainable consumer behavior in Italy. Full article
(This article belongs to the Section Foods of Marine Origin)
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13 pages, 2506 KB  
Article
Untargeted Metabolomics Reveals Distinct Serum Metabolic Profiles in Avian Influenza Occupational Exposure Populations
by Shuoqin Mao, Lei Wang, Jing Su, Caihua Long, Muti Mahe, Zhenguo Gao and Jia Liu
Metabolites 2025, 15(10), 663; https://doi.org/10.3390/metabo15100663 (registering DOI) - 11 Oct 2025
Viewed by 51
Abstract
Background and Objectives: Avian influenza poses a continuous public health threat, particularly to individuals with occupational exposure to poultry such as farm workers, live animal market employees, and processing plant staff. This study aimed to investigate the systemic metabolic effects of such exposure [...] Read more.
Background and Objectives: Avian influenza poses a continuous public health threat, particularly to individuals with occupational exposure to poultry such as farm workers, live animal market employees, and processing plant staff. This study aimed to investigate the systemic metabolic effects of such exposure and to identify potential biomarkers for early detection and health risk assessment. Materials and Methods: An untargeted liquid chromatography–mass spectrometry (LC-MS)-based metabolomics approach was applied to analyze serum samples from occupationally exposed individuals and healthy controls. Multivariate statistical analysis, pathway enrichment, and topology analysis were performed to identify significantly altered metabolites and metabolic pathways. The least absolute shrinkage and selection operator (LASSO) algorithm was employed to select key metabolites. Results: Multivariate statistical analysis revealed a clear separation between the exposure group and control, suggesting distinct metabolic profiles between the two populations. Pathway analysis indicated significant alterations in alanine, aspartate, and glutamate metabolism, as well as tryptophan metabolism, which are closely linked to immune regulation, energy metabolism, and host–pathogen interactions. LASSO feature selection and subsequent manual verification identified 17 key metabolites with strong discriminative power. Furthermore, lipidomic profiling revealed a pronounced increase in lysophosphatidylcholine (LPC) levels and a concurrent decrease in phosphatidylcholine (PC) species in exposed individuals. Conclusions: This study reveals metabolic disruptions associated with occupational avian influenza exposure and identifies potential serum biomarkers related to immune and lipid metabolism. These findings provide novel insights into host responses to avian influenza exposure and may support early detection and health risk assessment in high-risk occupational populations. Full article
(This article belongs to the Section Advances in Metabolomics)
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9 pages, 751 KB  
Communication
Seroprevalence of Anti-Neospora caninum and Anti-Toxoplasma gondii Antibodies in Cattle Intended for Human Consumption in the State of Paraíba, Brazil
by Geraldo Moreira Silva Filho, Jordania Oliveira Silva, Audisio Alves Costa Filho, Roberta Nunes Parentoni, Arthur Willian Lima Brasil, Thais Ferreira Feitosa and Vinícius Longo Ribeiro Vilela
Ruminants 2025, 5(4), 48; https://doi.org/10.3390/ruminants5040048 (registering DOI) - 11 Oct 2025
Viewed by 47
Abstract
We aimed to assess the seroprevalence of anti-Neospora caninum and anti-Toxoplasma gondii antibodies in cattle intended for human consumption in Paraíba, Brazil, and the associated risk factors. A total of 110 serum samples from slaughtered cattle were analyzed using the Indirect [...] Read more.
We aimed to assess the seroprevalence of anti-Neospora caninum and anti-Toxoplasma gondii antibodies in cattle intended for human consumption in Paraíba, Brazil, and the associated risk factors. A total of 110 serum samples from slaughtered cattle were analyzed using the Indirect Fluorescence Antibody Test (IFAT), with cut-off points of 1:200 for N. caninum and 1:64 for T. gondii. Seroprevalence was 8.2% (9/110) for N. caninum (titers 1:200–1:6400) and 18.2% (20/110) for T. gondii (titers 1:64–1:512). Risk factor analysis revealed that the variable female sex (cows) and the extensive farming system were statistically significantly associated with seroprevalence for N. caninum. Whereas for T. gondii, extensive farming, frequent animal purchase, and the lack of separation between calves and adult cattle were statistically significant. These findings demonstrate the circulation of these parasites in herds, with implications for animal and public health, indicating a potential risk of transmission to definitive hosts and humans through the consumption of raw or undercooked infected meat. Full article
(This article belongs to the Special Issue Parasitological Diagnosis and Alternative Control in Ruminants)
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15 pages, 827 KB  
Article
Development of a Simulation Model to Evaluate Dairy Production Systems in Northern Ireland
by Austen Ashfield, Michael Wallace and Claire Jack
Dairy 2025, 6(5), 57; https://doi.org/10.3390/dairy6050057 (registering DOI) - 11 Oct 2025
Viewed by 72
Abstract
Profitable dairy farming requires continuous appraisal and adaptation of production systems in response to changing market and agricultural policy conditions. Geopolitical and climate events have exemplified the exposure of farm incomes to the increased volatility associated with often-global market factors. In this context, [...] Read more.
Profitable dairy farming requires continuous appraisal and adaptation of production systems in response to changing market and agricultural policy conditions. Geopolitical and climate events have exemplified the exposure of farm incomes to the increased volatility associated with often-global market factors. In this context, bio-economic models can be a useful tool for researchers seeking to understand the financial resilience of different production systems to these changing circumstances. The AFBI Dairy Systems Model is presented and used to simulate the impacts of alternative price scenarios for Northern Ireland-based dairy systems. The whole farm model consists of four interdependent components, comprising farm system, animal nutrition, feed supply and financial sub models. The model is used to evaluate how fluctuations in milk, concentrate, fertiliser, contractor, and electricity prices, as well as interest rate changes, affect three distinct production systems. The financial performance of all systems was sensitive to variations in milk and concentrate prices but relatively insensitive to variations in fertiliser, contractor, and electricity prices and interest rate changes. The profitability of a low-output system was less exposed to variations in prices. In contrast, a high-output system was more exposed to price variations. However, a medium-input system was the most profitable across the majority of price scenarios investigated. Full article
(This article belongs to the Section Dairy Farm System and Management)
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19 pages, 2224 KB  
Article
Enhanced Biogas Production and Pathogen Reduction from Pig Manure Through Anaerobic Digestion: A Sustainable Approach for Urban Waste Management in Abidjan, Côte d’Ivoire
by Alane Romaric N’guessan, Youan Charles Tra Bi, Edi Guy-Alain Serges Yapo, Akeyt Richmond Hervé Koffi, Franck Orlando Yebouet, Alessio Campitelli, Boko Aka and N’Dédé Théodore Djeni
Clean Technol. 2025, 7(4), 89; https://doi.org/10.3390/cleantechnol7040089 (registering DOI) - 11 Oct 2025
Viewed by 64
Abstract
In Abidjan, the treatment of pig waste is becoming a priority given the continued growth of pig farms, which readily reuse manure as organic fertilizer. This study evaluated the effectiveness of anaerobic digestion for simultaneous biogas production and pathogen reduction from pig farm [...] Read more.
In Abidjan, the treatment of pig waste is becoming a priority given the continued growth of pig farms, which readily reuse manure as organic fertilizer. This study evaluated the effectiveness of anaerobic digestion for simultaneous biogas production and pathogen reduction from pig farm residues. Two 1600 L biodigesters were installed at pig farms in Port Bouët (PBk) and Abobo (Ab). They were fed with pig manure and water (1:4 ratio) and monitored over 56 days. The total biogas production was 22.63 m3 and 16.31 m3 for the PBk and Ab digesters, respectively, with peak production occurring between days 14 and 28. Following biofilter treatment, the methane content increased to 80–82%, yielding potential energy outputs of 2.32–3.29 kWh/d, with optimal production occurring at a pH of 7.28–7.76. The COD, BOD5, organic acid, and total nitrogen levels decreased progressively in the biodigesters, while the mineral element content remained almost unchanged. Complete elimination was achieved for most of the bacteria tested (E. coli, Enterococcus, Salmonella, etc.). However, Bacillus and Clostridium were able to persist, albeit with significant reductions of between 3.11 and 5.79 log10. Anaerobic digestion is an effective method of combining waste treatment and energy recovery. It eliminates major pathogens while producing valuable biogas. This makes it a sustainable waste management solution for urban agricultural systems. Full article
(This article belongs to the Special Issue Biomass Valorization and Sustainable Biorefineries)
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12 pages, 1242 KB  
Article
Gastrointestinal Parasite Infections in Beef Cattle: A Comparative Study Between Conventional and Holistic Farms in Alentejo, Portugal
by Cátia Gomes, João Lozano, Mariana Louro, Lídia Gomes, José Luís Castro, Leonor Duarte, Feliciano do Carmo Reis and Luís Madeira de Carvalho
Vet. Sci. 2025, 12(10), 970; https://doi.org/10.3390/vetsci12100970 - 10 Oct 2025
Viewed by 135
Abstract
Cattle are commonly affected by gastrointestinal (GI) parasites, which impact their welfare and productivity. Alternative management practices are currently being applied in several livestock farms worldwide to minimise or avoid the use of anthelmintic drugs on livestock in an unsustainable way. This study [...] Read more.
Cattle are commonly affected by gastrointestinal (GI) parasites, which impact their welfare and productivity. Alternative management practices are currently being applied in several livestock farms worldwide to minimise or avoid the use of anthelmintic drugs on livestock in an unsustainable way. This study aimed to compare the presence of GI parasites between conventional and holistic management farms in Alentejo, Portugal. Between January and April 2024, a total of 95 faecal samples were collected from adult female beef cattle belonging to four conventional management farms and four holistic management farms. Parasitological diagnosis included the Mini-FLOTAC method, Willis flotation, natural sedimentation, modified Ziehl–Neelsen technique, and faecal cultures. Higher egg shedding levels were found at holistic management farms, and strongyles were the most frequent GI parasites (92.6%). In addition, Oesophagostomum spp. (37%) and Trichostrongylus spp. (32%) were the most frequent genera, although no significant differences were found between the two management systems. Moreover, animals from both groups presented low shedding values (<200 eggs per gram of faeces, EPG). These preliminary results allow us to suggest that adopting holistic management practices may be a sustainable solution for the control of GI parasite infections in cattle farms. Full article
(This article belongs to the Special Issue Detection of Parasitic Diseases in Livestock)
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22 pages, 3537 KB  
Article
Enhanced Treatment of Swine Farm Wastewater Using an O3/Fe2+/H2O2 Process: Optimization and Performance Evaluation via Response Surface Methodology
by Hang Yu, Kexin Tang, Jingqi Li, Linxi Dong, Zuo Tong How, Dongming Wu and Rui Qin
Separations 2025, 12(10), 277; https://doi.org/10.3390/separations12100277 - 10 Oct 2025
Viewed by 184
Abstract
Biologically treated swine farm wastewater still contains high levels of refractory organics, humic substances and antibiotic residues, posing environmental risks and limiting opportunities for water reuse. Wastewater treatment by ozonation alone suffers from low mass transfer efficiency and selective oxidation. To overcome these [...] Read more.
Biologically treated swine farm wastewater still contains high levels of refractory organics, humic substances and antibiotic residues, posing environmental risks and limiting opportunities for water reuse. Wastewater treatment by ozonation alone suffers from low mass transfer efficiency and selective oxidation. To overcome these limitations, a catalytic ozonation process (O3/Fe2+/H2O2) was applied and optimized using Response Surface Methodology (RSM) based on single-factor experiments and Central Composite Design (CCD) for advanced swine farm wastewater treatment. The optimal conditions ([O3] = 25.0 mg/L, [Fe2+] = 25.9 mg/L, [H2O2] = 41.1 mg/L) achieved a COD removal of 44.3%, which was 86.8% higher than that of ozonation alone, and increased TOC removal to 29.5%, indicating effective mineralization. Biodegradability (BOD5/COD) of swine farm wastewater effluent increased from 0.01 to 0.34 after the catalytic ozonation treatment. Humic-like and fulvic-like substances were removed by 93.7% and 95.4%, respectively, and antibiotic degradation was significantly accelerated and enhanced. The synergistic process improved ozone utilization efficiency by 33.1% and removed 53.95% of total phosphorus through Fe3+-mediated coprecipitation. These findings demonstrate that with catalytic ozone decomposition and production of hydroxyl radicals, the O3/Fe2+/H2O2 system effectively integrates enhanced ozone utilization efficiency, radical synergy, and simultaneous pollutant removal, providing a cost-effective and technically feasible strategy for advanced swine farm wastewater treatment and safe reuse. Full article
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