Search Results (3,221)

Plant-derived bioactive metabolites have emerged as promising modulators of oxidative stress and inflammation, two interconnected processes involved in the pathogenesis of numerous chronic diseases. Arid ecosystems, particularly the Sonoran Desert, constitute an underexplored source of structurally diverse phytochemicals with significant pharmacological potential. This review provides a comprehensive overview of major classes of plant-derived bioactives, including polyphenols, flavonoids, terpenoids, and alkaloids, with emphasis on their molecular mechanisms of antioxidant and anti-inflammatory action. These compounds exert cytoprotective effects through direct reactive oxygen species (ROS) scavenging and indirect regulation of endogenous defense systems, primarily via activation of the Nrf2/Keap1 pathway and suppression of NF-κB signaling. Additional pathways, including MAPK, PI3K/Akt, AMPK, and mitochondrial regulatory networks, are discussed as critical mediators of redox balance and inflammatory control. Particular attention is given to Sonoran Desert plant species such as Bucida buceras, Phoradendron californicum, Larrea tridentata, Opuntia spp., and Agave deserti, all of which demonstrate promising biological activities associated with enhanced adaptation to environmental stress. Experimental approaches used to evaluate phytochemical bioactivity, including chemical assays, cellular models, omics technologies, and translational strategies, are also examined. Furthermore, this review discusses current limitations related to bioavailability, phytochemical variability, and clinical validation, highlighting emerging nanodelivery systems and precision medicine approaches as potential solutions. Collectively, the evidence supports the therapeutic relevance of Sonoran Desert plant bioactives as multi-target agents for modulating oxidative stress, inflammation, and chronic disease progression Full article

Salvia sclarea L. extract contains various bioactive components such as flavonoids and fatty acids, exhibiting anti-inflammatory, antioxidant, and antibacterial properties. This study aimed to investigate the effects of Salvia sclarea L. extract on the gut microbiota and serum metabolome in lambs. Sixty 2-month-old Chinese Merino female lambs (body weight 20 ± 2 kg) were randomly assigned to five groups. The control (CK) group received the basal diet only, while the treatment groups received the basal diet supplemented with 0.04 mL/kg (CL1), 0.08 mL/kg (CL2), 0.12 mL/kg (CL3), and 0.16 mL/kg (CL4) of Salvia sclarea L. extract, respectively. The results showed that Firmicutes, Bacteroidetes, Spirochaetes, and Proteobacteria were identified as the dominant phyla across all groups (>90%). Compared with the CK group, CL1 and CL2 groups significantly reduced the relative abundance of Tenericutes (decreased by 38.2% and 32.9%, respectively, p < 0.05); the relative abundance of Patescibacteria in the CL1 group was significantly lower (decreased by 55.2%, p < 0.05). At the genus level, Ruminococcaceae constituted a substantial proportion, including Ruminococcaceae UCG-005, UCG-010, UCG-014, and NK4A214 group. STAMP analysis revealed that Klebsiella was significantly enriched in CL2, CL3, and CL4 groups compared to the CK group (p < 0.05). Correlation analysis between microbiota and immune indices showed that Christensenellaceae R-7 group was significantly negatively correlated with TNF-α (p < 0.05); Ruminococcaceae UCG-005 was significantly negatively correlated with IFN-γ (p < 0.05) and showed a negative correlation trend with immunoglobulins (IgA, IgG, IgM). Conversely, Ruminococcaceae UCG-014 was significantly positively correlated with IL-4 (p < 0.05) but showed a negative correlation trend with IgM. Untargeted metabolomics analysis identified 8, 18, 25, and 20 differential metabolites in CL1, CL2, CL3, and CL4 groups, respectively. Notably, 3-hydroxy-7-methoxyflavone and Gamma-Glu-Cys were significantly upregulated across all treatment groups. KEGG pathway enrichment analysis indicated that these differential metabolites were primarily involved in nucleotide metabolism, fatty acid biosynthesis, and oxidative stress-related pathways. Further Spearman correlation analysis revealed significant associations between gut microbiota and differential metabolites. Specifically, g_Klebsiella was significantly positively correlated with 3-Hydroxycapric acid and 3-hydroxy-7-methoxyflavone (p < 0.05). In conclusion, Salvia sclarea L. extract modulates host energy metabolism by regulating nucleotide metabolism and fatty acid biosynthesis, and enhances immune function by alleviating oxidative stress, through the remodeling of gut microbiota and serum metabolome. Full article

Propolis is a resinous bee product with a long history of medicinal use, valued for its antimicrobial, antioxidant and anti-inflammatory properties. Identification of its key bioactive constituents would enable chemical standardization and quality control of propolis-based products, once their activity is confirmed. In the current study we investigated a Greek propolis sample (PR09) belonging to a phenolic-rich type, dominated by flavonoids. After extraction and fractionation with Fast Centrifugal Chromatography (FCPC), the fractions were evaluated for their DPPH and collagenase inhibitory activity while their NMR metabolic profiles were recorded. NMR HeteroCovariance Approach (NMR-HetCA) analysis of PR09 propolis methanolic extract revealed the presence of 26 secondary metabolites: seven diterpenes, 13 flavonoids and six caffeic acid esters. All compounds were identified from NMR-HetCA and Statistical Total Correlation Spectroscopy (STOCSY) plots prior to their isolation. NMR-HetCA analysis indicated that caffeic acid derivatives were the most potent inhibitors of the DPPH free radical and collagenase. Additionally, galangin (11) and 3-O-methyl galangin (24) appeared to contribute considerably to the antioxidant activity, while together with pinocembrin (12), they all contributed to the extract’s collagenase inhibitory activity. In contrast, metabolites such as isocupressic acid (8), 13-epi-cupressic acid (18), pinostrobin (17) and chrysin (7) appeared not to contribute to the observed activities. Bioassays of selected metabolites confirmed the NMR-HetCA’s predictions, with caffeic acid phenethyl ether (1) exhibiting very high inhibition (92.54 ± 0.16%), and notable collagenase inhibition close to 50% (at 100 μg/mL). Overall, the findings demonstrate that NMR-HetCA enables rapid identification of bioactive compounds in propolis extracts and is proposed as a tool in accelerating the evaluation of propolis samples prior to laborious isolation procedures. Full article

The genus Cymbopogon comprises neocosmopolitan grasses widely used as medicinal plants and in the perfume, pharmaceutical and herbal product industries. Despite their economic relevance, these species are still considered orphan crops, with limited phytotechnical, genomic and evolutionary studies within the Poaceae family. In this study, we investigated the evolutionary relationships of Cymbopogon flexuosus and Cymbopogon winterianus, with a focus on differences in gene expression associated with the biosynthesis of secondary metabolites. De novo transcriptome assembly yielded 25,576 transcripts in C. flexuosus and 42,250 in C. winterianus. A total of 5318 and 8631 more informative differentially expressed transcripts (DETs) were identified in each mapping, among which 76 and 94 were associated with secondary metabolism pathways. When mapping the libraries against related species, the highest percentages of mapped reads per transcriptome and per gene (depth) were observed in Andropogon gerardi, Sorghum bicolor, Saccharum officinarum, Miscanthus sinensis, Miscanthus lutarioriparius and Zea mays. These results indicate A. gerardi, S. bicolor and Z. mays as the most promising genomic models for future studies within the genus Cymbopogon. Comparison of the expression of transcripts that are homologous to the precursor enzymes of terpenoids, phenylpropanoids, flavonoids and other secondary metabolites revealed a complex and non-linear interaction between the metabolic pathways in each species and it was not possible to predict the predominance of greater expression of a class of metabolites on a given species. Full article

Phytoestrogens are plant-derived phenolic compounds that structurally resemble endogenous estrogens and can exert both estrogenic and anti-estrogenic effects in animals. In ruminant nutrition, the main classes of phytoestrogens (isoflavones, lignans, stilbenes, coumestans and selected flavonoids) are supplied predominantly by legume forages and soybean-based feeds, in which concentrations can reach several mg/g of dry matter. After ingestion, these compounds are extensively metabolized by the rumen microbiota to derivatives with altered biological potency, such as equol and p-ethyl-phenol, which influence endocrine, immune and metabolic pathways. Experimental and field studies in cattle, sheep and goats indicate that dietary phytoestrogens may improve nitrogen utilization, immune competence, growth performance, antioxidant status and milk yield. However, they can also impair fertility, modify hormone profiles and compromise embryo survival in a compound-, dose-, and species-dependent manner. In this review, we summarize current knowledge on the botanical and nutritional sources, ruminal metabolism and transfer of phytoestrogens in ruminants, and critically examine their effects on blood metabolites, immune responses, growth and carcass traits and lactational performance and reproductive function. A structured literature search based on PRISMA principles was used to identify and appraise experimental and observational studies in both grazing and intensive production systems up to 2025. Remaining knowledge gaps and practical implications for the safe use of phytoestrogen-rich feeds in livestock production are highlighted. Full article

Euphorbia clavarioides Boiss. is traditionally used in wound healing and other medicinal applications. Its bioactive compounds and pharmacological potential remain underexplored. This study investigated the phytochemical composition, antioxidant, anti-inflammatory, and anticancer activities of E. clavarioides Boiss. traditionally used in wound healing. Plant extracts were characterized using phytochemical screening, Fourier-transform infrared spectroscopy (FTIR), and liquid chromatography–mass spectrometry (LC-MS). Antioxidant activity was evaluated via DPPH and nitric oxide (NO) scavenging assays, anti-inflammatory effects through nitrite inhibition in LPS-stimulated RAW 264.7 macrophages, and anticancer potential using the MTT assay against DU-145, PC-3, SKU-T, and AGS cell lines. Phytochemical screening confirmed tannins, phlobatannins, saponins, flavonoids, alkaloids, steroids, terpenoids, and cardiac glycosides. FTIR spectra of aqueous extracts revealed peaks at 2990.66 cm⁻¹ (O–H), 1738.68 cm⁻¹ (C=O), 1217.22 cm⁻¹ (C–N), and 527.37 cm⁻¹ (C–Cl). LC-MS profiling identified diverse metabolites, including phenolics (pseudolaroside B, cinnamtannin A2, (−)-medicarpin, butyrolactol A) and terpenoids (zerumbone, sclareol isomer, diterpenoid-like compounds), underpinning the plant’s bioactivity. Methanol extracts exhibited the strongest DPPH scavenging activity (IC₅₀ = 755.71 µg/mL), whereas aqueous and ethanol extracts demonstrated superior NO scavenging. Ethanol extracts showed maximal anti-inflammatory activity, while aqueous extracts induced pro-inflammatory effects. Cytotoxicity assays indicated negligible toxicity. In anticancer assays, ethanol and methanol extracts significantly inhibited the proliferation of all tested cell lines at 100 µg/mL, exceeding drug control, whereas aqueous extracts displayed lower activity. The bioactive compounds in E. clavarioides support its traditional wound-healing use and demonstrate mechanistic antioxidant, anti-inflammatory, and anticancer activities, highlighting its potential as a source of multi-target natural therapeutics. Full article

Guaje (Leucaena leucocephala), mezquite (Prosopis laevigata), and guamuchil (Pithecellobium dulce) are leguminous trees distributed throughout southeastern Mexico. Their pods and seeds constitute the main agroecological residues and represent a natural source of secondary metabolites with high biotechnological potential. The aim of this study was to determine the chemical composition, antimicrobial and antioxidant activities, and toxicity of the pods and seeds of L. leucocephala, P. laevigata, and P. dulce. It was found that pod extracts contained higher concentrations of phenolic compounds, flavonoids, and terpenes than seed extracts. Antimicrobial assays showed inhibition zones ranging from 8.1–14.7 mm (E. coli), 8.8–15.1 mm (S. aureus), 11.3–15.4 mm (E. faecalis), 8.9–24.1 mm (C. albicans), and 8.5–22.6 mm (C. krusei). The ethyl acetate (AVPD) and ethanolic (EVPD) extracts from P. dulce pods showed the highest antimicrobial activity, with MIC values ranging from 0.03 to 0.15 mg/mL, MBC values of 0.07 mg/mL (S. aureus and E. faecalis), and MFC values of 1.25 mg/mL (C. albicans) and 0.62 mg/mL (C. krusei). Antioxidant activity was higher in pod extracts, with AVPD and EVPD showing IC₅₀ values of 0.257 and 0.320 mg/mL, respectively. Consistently, EVPD exhibited the highest phenolic content (133.24 mg GAE/g) and flavonoid content (50.90 mg QE/g), followed by AVPD (87.29 mg GAE/g and 42.40 mg QE/g, respectively). The results indicate that pod extracts of L. leucocephala and P. dulce contain secondary metabolites with broad antimicrobial and antioxidant potential and low toxicity. Full article

Pseudomonas fluorescens is a major psychrotrophic bacterium responsible for spoilage in refrigerated foods, particularly dairy products, where deterioration is driven by biofilm formation, quorum sensing (QS) regulation, and the secretion of thermostable lipases. Conventional control strategies reduce bacterial loads but often fail to prevent enzymatic spoilage. Plant-derived phenolic compounds have been widely reported as QS inhibitors and lipase modulators in various biological systems; however, evidence specifically addressing their effects on P. fluorescens regulatory networks and bacterial lipases remains limited. This review critically examines current knowledge on QS-mediated biofilm formation and lipase production in P. fluorescens and analyzes the reported inhibitory activity of phenolic compounds, with emphasis on oak (Quercus spp.)-derived metabolites. While flavonoids and phenolic acids such as quercetin, gallic acid, and p-coumaric acid have demonstrated QS inhibition and antilipolytic activity in other Pseudomonas species and pancreatic models, direct mechanistic validation in P. fluorescens lipases is scarce. Moreover, most studies rely on crude plant extracts without comprehensive metabolomic characterization, and the potential contribution of additional oak metabolites, including terpenoids, remains largely unexplored. Identifying these gaps is essential for advancing toward integrative approaches that combine enzymology, molecular modeling, and validation in food-relevant systems. Full article

Sprout vegetables have emerged as functional instant foods, with elevated concentrations of bioactive compounds compared with their mature counterparts. Chinese kale (Brassica oleracea var. alboglabra) is a cruciferous Brassica vegetable particularly rich in phenolic compounds, glucosinolates, and other nutrients, making it a suitable candidate for sprout production. This study aimed to explore the impact of melatonin (MT), calcium chloride (CaCl₂), and their combination on the quality and functional metabolism of Chinese kale. The results showed that MT treatment alone led to significantly higher ferric-reducing antioxidant power and concentrations of chlorophylls, carotenoids, soluble sugar, soluble protein, flavonoid, total phenolic compounds, and glucosinolates than those under CaCl₂ treatment alone. CaCl₂ treatment alone increased ascorbic acid content by 30.5%, but had limited effects on protein accumulation and secondary metabolites. However, the combined treatment did not exert a synergistic effect on ascorbic acid content, which decreased by 19.8% compared with that under the control treatment, significantly (p < 0.05). Overall, MT treatment was effective in boosting nutrient levels, thereby elevating the functional quality of Chinese kale sprouts. Full article

The role of medicinal plants in primary healthcare and livelihoods around the world is both ancient and well-documented. Agathosma betulina (P.J. Bergius) Pillans, commonly known as ‘buchu’, has long been utilised in traditional medicine as a household remedy for various ailments and is also valued for its essential oils in the cosmetics and pharmaceutical industries. This study aimed to profile and quantify the secondary metabolites in buchu using ultra-performance liquid chromatography quadrupole time-of-flight combined with mass spectrometry (UPLC-QTOF-MS) techniques, whereby plant material from three distinct locations in the Western Cape Province, Groot Winterhoek, Citrusdal, and Cederberg, was collected. A total of 32 maker compounds were identified from buchu leaves. The results revealed a significant location-dependent variation in the accumulation of multiple classes of phytochemicals, including phenolic acids, flavonoids, saponins, terpenoids, oligosaccharides, vitamins, and steroids. Citrusdal samples had the most bioactive compounds compared to the Cederberg and Groot Winterhoek. Citrusdal had the highest flavonoid levels, while Cederberg samples were the richest in phenolic acids and Groot Winterhoek was dominant in iridoid glycoside levels. Principal component analysis (PCA) revealed distinct clusters corresponding to the three different regions, confirming chemical differences. Elucidating the distribution of secondary metabolites in this species may provide new information for possible medicinal and pharmacological uses, such as the creation of novel and enhanced organic medications and food products. These results will aid in selecting a buchu chemotype with optimal attributes for the intended therapeutic application, helping to protect wild populations from over-exploitation through cultivation. Full article

Water stress is a major abiotic constraint limiting the growth and productivity of alfalfa (Medicago sativa L.). To elucidate the adaptive mechanisms and identify key drought-tolerance genes, physiological measurements were integrated with multi-omics analyses of cultivar ‘Tamu 1’ under three water treatments: waterlogging (100% field water capacity), normal irrigation (80% FWC), and drought (light: 60% FWC, moderate: 40% FWC, severe: 20% FWC). Water stress markedly inhibited plant growth, induced oxidative stress, and reduced the photosynthetic capacity. Compared with waterlogging stress (DAMs: n = 71; DEGs: n = 313), drought stress resulted in a substantially greater number of differentially accumulated metabolites (DAMs, n = 1504) and differentially expressed genes (DEGs, n = 8006). Weighted gene co-expression network analysis (WGCNA) identified six key modules and ten hub genes associated with stress responses. Integrated transcriptomic and metabolomic analyses further revealed four major responsive pathways: starch and sucrose metabolism, phenylpropanoid and flavonoid metabolism, glutathione metabolism, and zeatin biosynthesis. Based on integrative criteria, including differential expression (|log₂FC| ≥ 1, adjusted p < 0.05), WGCNA modules significantly associated with drought-related traits (R² > 0.6), as well as functional annotation and protein–protein interaction (PPI) network topology, 28 candidate genes associated with drought tolerance were identified, of which six were further validated by quantitative real-time PCR (qRT-PCR). These findings highlight key metabolic pathways and regulatory modules underlying alfalfa responses to water stress and provide valuable candidate gene resources for improving drought tolerance. Full article

Samsoniella hepiali produces an array of pharmacologically valuable metabolites, but how environmental pH regulates its antioxidant system and lipid metabolism during submerged fermentation remains unclear. This study aimed to investigate the effects of different initial culture pH values (pH 4, 5, 6, and 7) on the antioxidant capacity and lipidomic metabolism of S. hepiali. The results demonstrated that at pH 5, the activities of peroxidase (POD) and superoxide dismutase (SOD), the contents of total phenolics (TP) and flavonoids, the scavenging rates of DPPH• and •OH, and the total antioxidant capacity all peaked. Conversely, the level of glutathione (GSH) reached its maximum at pH 6 (0.69 ± 0.014 μmol/g). Lipidomic analysis identified a total of 404 lipid molecular species, mainly TG, PE, and DG. Comparative analysis among pH 4 vs. pH 5, pH 6 vs. pH 5, and pH 7 vs. pH 5 revealed 27 core DALs belonging to 11 lipid subclasses, most of which were upregulated at pH 5. KEGG pathway enrichment analysis further revealed that sphingolipid metabolism was the sole core co-enriched pathway under different pH conditions. Particularly at pH 5, key signaling lipids, such as ceramides, underwent pronounced targeted accumulation. This study elucidates the molecular adaptation mechanisms of medicinal fungi in response to pH variation from a lipidomic perspective. It provides a basis for optimizing fermentation conditions to enhance antioxidant activity and functional lipid production. Full article

In plant factories with artificial lighting (PFALs), spectral regulation serves as the predominant factor governing plant growth and development. The implementation of red-enriched spectral regimens during cultivation promotes biomass accumulation, whereas blue-dominant spectra enhance the biosynthesis of phytochemicals and nutritional compounds in plants. Nevertheless, systematic investigations into the effects of staged spectral regimens on both plant development and secondary metabolite biosynthesis remain limited. This study evaluated four distinct stage-specific dynamic lighting regimens (T1–T4) under a constant total photosynthetic photon flux density (PPFD) of 200 μmol·m⁻²·s⁻¹. The treatments utilized three distinct red-to-white photon flux ratios (R:W = 3:1, 1:1, and 1:3) administered sequentially during critical developmental phases of Pak choi: the seedling stage, the early growth stage (15 days after transplanting, DAT), and the late growth stage (16–30 DAT). The effects of these treatments on biomass production, morphological development, photosynthetic pigments, nutritional metabolites, antioxidant levels and radical quenching capacity were evaluated. The results demonstrated that the T4 treatment significantly enhanced biomass production, increasing shoot fresh weight by 51.3% compared to the T1 treatment at the late growth stage. The application of a higher red-light proportion (HR, R:W = 3:1) during the seedling stage significantly increased leaf area by 70% compared to the low red-light treatment (LR, R:W = 1:3). Regarding nutritional quality, while carotenoid content showed no significant differences among treatments, higher blue-light proportions selectively stimulated the biosynthesis of chlorophyll, vitamin C, and soluble proteins. Specifically, the T3 treatment enhanced certain traits during the early growth stage, whereas the T2 treatment best maintained specific antioxidant capacities (FRAP and flavonoids) at the late growth stage prior to harvest. Notably, nitrate levels were not significantly affected by the spectral shifts. This study establishes that the temporal modulation of red-to-white spectral ratios enables the targeted optimization of either crop yield (T4) or specific harvest-stage nutritional attributes (T2) in Pak choi. Full article

To elucidate the impacts of climatic and edaphic factors on the chemical composition and bioactivities of black mulberries, this study conducted a systematic comparative analysis of fruits sourced from four regions in Xinjiang: Turpan (T), Bayingolin Mongol Autonomous Prefecture (B), Hotan (H), and Kashgar (K). H exhibited the highest contents of total phenolics and total tannins, whereas T showed elevated levels of total flavonoids and flavan-3-ols. Notably, samples from B and H demonstrated superior antioxidant potential. Using UPLC-MS/MS, a total of 48 anthocyanin metabolites and 4 non-anthocyanin metabolites were identified in the mulberry fruits. Among these, 6 were region-specific compounds, and 8 were identified as differential metabolites. Furthermore, headspace solid-phase microextraction (HS-SPME) coupled with GC-MS/MS revealed 292 volatile metabolites, of which 15 were identified as differential metabolites based on OPLS-DA and relative odor activity value (rOAV) analyses. Metabolite profiling indicated that B possessed the greatest diversity of volatile metabolites, while T exhibited a remarkable richness in anthocyanin diversity. The observed regional variations in chemical constituents and metabolite profiles collectively accounted for the differences in antioxidant capacity and enzyme inhibitory activities among the black mulberry fruits. These findings provide a theoretical foundation for the regional characterization and targeted processing of black mulberries from the four production areas in Xinjiang. Full article

Spatholobus suberectus is a medicinal and edible plant widely recognized for its pharmacological potential. Although its stems have been extensively studied and utilized, its leaves are often discarded as agricultural waste, leading to significant resource underutilization. To promote the sustainable valorization of these leaves, this study aimed to provide a predictive evaluation of their bioactive constituents and pharmacological potential. Leaves of S. suberectus were collected at six growth stages (January, March, May, July, September and November). A total of 6750 metabolites were identified, primarily comprising amino acids and derivatives (26.74%), organic acids (15.33%), and bioactive secondary metabolites, including flavonoids and phenolic acids (27.98%). Metabolic profiling revealed clear seasonal patterns, allowing the classification of the six harvest months into three distinct stages: January and March (G1), May and September (G2), and July and November (G3). Among these, the G1 stage was notably enriched in defensive secondary metabolites, particularly flavonoids and phenolic acids. To predict the bioactivity of these metabolites and elucidate potential mechanisms of action, network pharmacology and molecular docking analyses were employed. Network pharmacology and molecular docking were employed to predict anti-inflammatory mechanisms. From the metabolome, 83 potential bioactive compounds were screened, interacting with 306 targets. Network analysis identified 60 core anti-inflammatory targets (e.g., TNF, AKT1, PTGS2, STAT3) that were significantly enriched in MAPK and PI3K-Akt pathways. Molecular docking revealed strong binding affinities, with pelargonidin showing the highest affinity for PTGS2 (−11.72 kcal/mol). Candidate metabolites peaked in January, and extracts from this period exhibited notable COX-2 inhibitory activity (IC50 = 16.41 μg/mL). This research provides essential chemical characterization and preliminary bioactivity evidence to support the valorization of S. suberectus leaves and identifies January as the optimal harvest time to maximize their bioactive potential. Full article