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24 pages, 7070 KB  
Article
Spatiotemporal Dynamics, Spatial Spillover Effects, and Driving Mechanisms of Non-Grain Use of Cultivated Land in an Ecologically Fragile Region
by Yao Cui, Hongrui Sun, Yaolin Liu, Ligang Wang, Yanfang Liu, Rui An, Xinyue Zhang, Yifan Xie, Lin Zhang and Jiwei Xu
Land 2026, 15(6), 910; https://doi.org/10.3390/land15060910 (registering DOI) - 25 May 2026
Abstract
Non-grain use of cultivated land (NGUCL) in ecologically fragile regions has become a major challenge to food security and land sustainability, yet its spatiotemporal dynamics, spatial spillover effects, and associated factors remain insufficiently understood. Taking Ningxia, China, as a typical semi-arid to arid [...] Read more.
Non-grain use of cultivated land (NGUCL) in ecologically fragile regions has become a major challenge to food security and land sustainability, yet its spatiotemporal dynamics, spatial spillover effects, and associated factors remain insufficiently understood. Taking Ningxia, China, as a typical semi-arid to arid transition zone, this study developed a phenology-informed framework that combined multi-temporal Landsat imagery, random forest classification, spatial autocorrelation analysis, centroid and standard deviation ellipse models, and a spatial lag model to identify and analyze NGUCL in 2005, 2010, 2015, and 2020. Within the cultivated land boundary, NGUCL was further decomposed into cash crop-cultivated farmland (CCCF) and farmland abandonment (FA). The results show that the classification framework achieved robust performance, with overall accuracies above 85% across the benchmark years. Food-crop mapping reached an OA of 86.38–90.12% and a Kappa of 0.80–0.85, while FA mapping reached an OA of 85.60–86.74% and a Kappa of 0.70–0.72. NGUCL in Ningxia exhibited strong subregional differentiation under the gradients of northern irrigation, central arid, and southern mountainous conditions. CCCF was more closely associated with irrigated and agriculturally productive areas, whereas FA was concentrated in ecologically constrained counties and showed stronger dispersion and migration complexity. Spatial econometric results further indicate significant spatial spillover effects, suggesting that NGUCL-related processes in one county are associated with those in neighboring counties. The effects of natural, socioeconomic, and agricultural production factors also varied by type and period, indicating that NGUCL in ecologically fragile regions is not a homogeneous land-use transition process. By distinguishing CCCF from FA, this study provides a more nuanced interpretation of NGUCL and offers empirical evidence for understanding cultivated land transition and governance in ecologically fragile areas. Full article
(This article belongs to the Section Land Use, Impact Assessment and Sustainability)
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21 pages, 494 KB  
Article
Influence of Harvesting and Seasonal Variability on the Physicochemical and Antioxidant Properties of Native Bee (Tetragonisca fiebrigi) Honey from Bolivia’s Tropical Dry Forests
by Alejandra Romero-Padilla, Luís M. G. Castro, Manuela Pintado and María Emilia Brassesco
Molecules 2026, 31(11), 1819; https://doi.org/10.3390/molecules31111819 (registering DOI) - 25 May 2026
Abstract
This study evaluates the influence of harvesting methods and seasonal variability on the physicochemical and antioxidant properties of Tetragonisca fiebrigi honey produced in the tropical dry forest of Bolivia. Despite the growing interest in stingless bee honey, studies addressing the combined effects of [...] Read more.
This study evaluates the influence of harvesting methods and seasonal variability on the physicochemical and antioxidant properties of Tetragonisca fiebrigi honey produced in the tropical dry forest of Bolivia. Despite the growing interest in stingless bee honey, studies addressing the combined effects of seasonality and collection practices in this region remain scarce. Honey samples were collected during winter and spring using three approaches: conventional, optimized (based on good manufacturing practices), and direct racking from natural nests. Physicochemical parameters (pH 4.60–6.15; moisture 28-34%; water activity 0.69–0.75) and sugar composition (glucose 10.60–29.03 g/100 g; fructose 9.01–21.97 g/100 g; sucrose 0.70–3.23 g/100 g) showed variability primarily associated with season rather than harvesting method. Bioactive compounds exhibited a marked seasonal effect, with higher total phenolic content (up to 11.03 mg GAE/100 g), flavonoids (up to 23.08 mg QE/100 g), and antioxidant capacity (DPPH up to 1.33 mol TE/100 g; ORAC up to 25.93 mol TE/100 g) in spring samples. Multivariate analysis (PCA) revealed that honey variability is structured along bioactive and physicochemical axes, with samples obtained using the optimized method showing reduced dispersion and greater compositional consistency. These results indicate that while seasonality governs the compositional and functional properties of T. fiebrigi honey, improved harvesting practices contribute to reducing variability and enhancing product standardization. This study provides one of the first comprehensive datasets on Bolivian stingless bee honey and highlights its potential as a functional food, supporting the development of species-specific quality criteria and sustainable meliponiculture in tropical dry forest ecosystems. Full article
(This article belongs to the Special Issue Bioproducts for Health, 4th Edition)
16 pages, 4544 KB  
Review
Microbial Exopolysaccharides, Redox Modulation, and Antioxidant Activity in Fermented Foods
by Fares Boudjouan, Giorgia Perpetuini, Rosanna Tofalo, Yves Waché and Nadjet Benaida Debbache
Antioxidants 2026, 15(6), 665; https://doi.org/10.3390/antiox15060665 (registering DOI) - 25 May 2026
Abstract
Oxidative stress, caused by the excessive production of reactive oxygen and nitrogen species, contributes to cellular damage and chronic diseases. Fermented foods are increasingly recognized for their antioxidant properties, which are strongly influenced by microbial metabolism during fermentation. This review examines three major [...] Read more.
Oxidative stress, caused by the excessive production of reactive oxygen and nitrogen species, contributes to cellular damage and chronic diseases. Fermented foods are increasingly recognized for their antioxidant properties, which are strongly influenced by microbial metabolism during fermentation. This review examines three major microbial mechanisms involved in antioxidant enhancement in fermented foods: exopolysaccharide (EPS) production, release of matrix-bound bioactive compounds, and microbial modulation of redox conditions. Microbial EPS contribute through radical scavenging and metal chelation, while microbial enzymes increase the bioavailability of phenolic compounds, peptides, and other antioxidant molecules. In addition, microbial metabolic activity influences the redox environment of fermented systems through electron-transfer processes and reducing metabolites. By integrating these complementary mechanisms, this review provides a comprehensive framework linking microbial biotransformation and redox modulation to the antioxidant properties of fermented foods, and highlights their potential for the development of functional fermented products. Full article
(This article belongs to the Special Issue The Antioxidants in Fermented Foods—2nd Edition)
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26 pages, 5108 KB  
Systematic Review
INFOGEST 2.0 Protocol Applied to Animal-Derived Milk and Dairy Products: A Systematic Review of Six Years of Scientific Effort
by Giuseppe De Santis, Olubukunmi Amos Ilori, Diana Marisol Abrego-Guandique, Pierluigi Plastina, Paola Tucci and Erika Cione
Foods 2026, 15(11), 1871; https://doi.org/10.3390/foods15111871 - 25 May 2026
Abstract
The INFOGEST protocol is a standardised in vitro digestion model widely utilised to evaluate the digestibility and bioaccessibility of nutrients in diverse food matrices. This review focuses on its application since 2020 (after the publication of the INFOGEST 2.0 model) to milk and [...] Read more.
The INFOGEST protocol is a standardised in vitro digestion model widely utilised to evaluate the digestibility and bioaccessibility of nutrients in diverse food matrices. This review focuses on its application since 2020 (after the publication of the INFOGEST 2.0 model) to milk and dairy products, which often serve as a suitable food matrix in digestion studies. By analysing 50 studies selected using a semi-automated method, this review highlights its strong performance in reproducing general digestive trends, including peptide fingerprint profiling, consistent high-protein digestibility, and matrix-dependent lipid and mineral bioaccessibility. The model is particularly effective in evaluating structural modifications of dairy products and their impact on digestive behaviour. However, its application remains skewed toward bovine systems, limiting broader relevance to other dairy matrices. Methodological variability, including protocol modifications and emerging semi-dynamic adaptations, poses challenges to reproducibility. Furthermore, reliance on simplified downstream models constrains the physiological interpretation of bioactivity and nutrient absorption. Future progress requires harmonised dynamic extensions, expanded use of advanced biological systems, and inclusion of diverse dairy matrices. Collectively, these advances will support a shift from descriptive bioaccessibility toward more predictive assessments of nutrient bioavailability. This six-year, non-topic-dependent bibliometric analysis contextualises the expanding adoption of INFOGEST 2.0 as reflected in its versatility and evolving scope, positioning it as a cornerstone tool for advancing our understanding of dairy nutrition, digestion-derived bioactivity, and ultimately, the relationship between dairy consumption and human health. Full article
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28 pages, 2500 KB  
Review
Impact of Functional Feed Additives and Lower Antibiotic Use on Poultry Meat Quality and Consumer Perception
by Abdul Samad, Ayesha Muazzam, AMM Nurul Alam, SoHee Kim, ChanJin Kim, SiHoon An, Young-Hwa Hwang and Seon-Tea Joo
Foods 2026, 15(11), 1868; https://doi.org/10.3390/foods15111868 - 25 May 2026
Abstract
The poultry industry is undergoing a major transition to reduce the use of antibiotics, as a result of the growing concerns about antimicrobial resistance, antibiotic residue in meat and increasingly stringent regulatory policies. This trend has led to an increased interest in functional [...] Read more.
The poultry industry is undergoing a major transition to reduce the use of antibiotics, as a result of the growing concerns about antimicrobial resistance, antibiotic residue in meat and increasingly stringent regulatory policies. This trend has led to an increased interest in functional feed additives as potential alternatives that may support bird health, growth performance and meat quality. There are functional additives, including probiotics, prebiotics, synbiotics, phytogenics, organic acids, enzymes, essential oils, vitamins, minerals and postbiotics, that have shown potential effectiveness in enhancing gut health, nutrient utilization, immunity and disease resistance in poultry. The advantages that are frequently noticed are increased feed conversion ratio, body weight gain, carcass yield and improved meat quality characteristics, such as water-holding capacity, color stability, tenderness, oxidative stability and shelf life. Furthermore, the decrease in the use of antibiotics decreases the risk of residues and also the transmission of antimicrobial resistance genes through the food chain and the environment. Consumer interest in antibiotic-free and naturally raised poultry meat has also led to the emergence of premium market opportunities, where trust, transparency in poultry labelling and perceived safety are key drivers of consumer acceptance. But there are issues yet to be addressed regarding additive efficacy variability, dosage standardization, cost-effectiveness and implementation on farms under different production systems. This review critically evaluates the scientific evidence related to the use of functional feed additives as an alternative to antibiotics in poultry nutrition, focusing on their effects on meat quality, food safety, economic viability, sustainability and consumer perception. Precision nutrition, combinations of synergistic additives, and data-driven feed strategies will be key to future progress to enable profitable and sustainable poultry production. Full article
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21 pages, 2812 KB  
Article
Seasonal Shifts in the Microbiota of Wild-Caught Danish Carcinus maenas
by Lorenzo Chinellato, Lisbeth Truelstrup Hansen, Martin L. Kragh, Nina Gringer and Claus H. Bang-Berthelsen
Microorganisms 2026, 14(6), 1187; https://doi.org/10.3390/microorganisms14061187 - 25 May 2026
Abstract
Underutilized and abundant in the Danish coastal area, Carcinus maenas has become a threat to the environment and to the local fisheries. In this study we investigated the microbiota and presence of microbial hazards of interest for human health in crabs caught over [...] Read more.
Underutilized and abundant in the Danish coastal area, Carcinus maenas has become a threat to the environment and to the local fisheries. In this study we investigated the microbiota and presence of microbial hazards of interest for human health in crabs caught over the period of one year, to investigate its potential for human consumption. Between 2023 and 2024, four seasonal samples of live specimens (n = 5) were caught off the Lillebælt (DK) coastal area. To characterize the microbiota of the crabs, a culture-dependent approach was used to determine total aerobic mesophilic count, total aerobic psychrotrophic count, Bacillus spp., Enterobacteriaceae, lactic acid bacteria, fungi, Salmonella spp., Listeria monocytogenes and Bacillus cereus. MALDI-TOF was used to corroborate results and further identify isolated microorganisms. The results were then compared with data obtained from amplicon sequencing of community 16S rRNA genes to compare family-level compositions of the microbiota. Of the pathogens of interest, B. cereus was detected during summer/autumn, reaching a maximum of 2.5 log cfu/g. Salmonella spp., and L. monocytogenes were below the limit of detection (<1 cfu/0.1 g). Spoilage bacteria were detected (e.g., Brochothrix spp., Carnobacterium spp., Photobacterium spp., Pseudomonas spp., Psychrobacter spp. and Shewanella spp.). The study highlighted significant seasonal changes (PERMANOVA, FDR-adjusted p = 0.00003) in the microbial composition. The gathered evidence suggests that with proper handling, the crabs could represent a safe resource. Full article
(This article belongs to the Section Food Microbiology)
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31 pages, 10867 KB  
Article
Development and Optimization of Rice and Teff Based Gluten-Free Mixes for Traditional Algerian Pancakes: Evaluation of Technological Properties, Nutritional Quality, and Sensory Attributes
by Awatif Fetouhi, Hayat Bourekoua, Radia Ayad, Fairouz Djeghim, Meryem Bouchrit, Amina Mosbah, Khawla Kerbab, Maria D’Elia, Luca Rastrelli and Soued Cherak
Foods 2026, 15(11), 1867; https://doi.org/10.3390/foods15111867 - 25 May 2026
Abstract
Gluten-free fermented products remain technologically challenging due to the absence of gluten, which plays a key role in stabilizing batter structure and gas retention. This study proposes a mixture design-driven approach to develop gluten-free Algerian pancakes based on rice and teff formulations enriched [...] Read more.
Gluten-free fermented products remain technologically challenging due to the absence of gluten, which plays a key role in stabilizing batter structure and gas retention. This study proposes a mixture design-driven approach to develop gluten-free Algerian pancakes based on rice and teff formulations enriched with legumes and seeds, aiming to restore techno-functional properties while improving nutritional quality. Two formulations, a teff-based (TBF) and a rice-based (RBF) system, were optimized using a simplex centroid mixture design and evaluated in comparison with durum wheat pancakes. The results demonstrated that formulation strongly influenced batter rheology and final structure. The TBF system exhibited superior technological performance, with higher specific volume (1.77 cm3/g), lower density (0.56 g/cm3), and enhanced porosity, associated with improved protein and fiber content. In contrast, the RBF formulation showed higher antioxidant activity. The findings highlight the critical role of component interactions in modulating batter viscosity and foam stability, which directly affected pore development and product airiness. Both optimized formulations successfully reproduced the characteristic “light and airy” structure of traditional pancakes, achieving good sensory acceptability. Overall, this study demonstrates that mixture design can effectively guide the development of gluten-free fermented systems by linking composition, rheology, and structural properties, providing a strategy for improving the quality of traditional gluten-free foods. Full article
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24 pages, 19412 KB  
Article
Walnut Septum-Derived Aqueous Extract Alleviates Colitis Through Modulation of Gut Metabolism and Inflammatory Signaling
by Beier Jiang, Yu Wan, Lina Liu, Jiajun Cheng, Tianjiao Min, Xinlong Gao, Zicheng Yu, Li Ma and Ying He
Foods 2026, 15(11), 1866; https://doi.org/10.3390/foods15111866 - 25 May 2026
Abstract
The aqueous extract of Diaphragma Juglandis Fructus (AED), a by-product of Juglans regia L., represents a promising food-derived functional ingredient with potential benefits for intestinal health. This study evaluated the anti-colitis effects of AED and explored its underlying mechanisms using LPS-stimulated RAW264.7 macrophages [...] Read more.
The aqueous extract of Diaphragma Juglandis Fructus (AED), a by-product of Juglans regia L., represents a promising food-derived functional ingredient with potential benefits for intestinal health. This study evaluated the anti-colitis effects of AED and explored its underlying mechanisms using LPS-stimulated RAW264.7 macrophages and a DSS-induced colitis mouse model. In DSS-induced colitis in mice, AED at 10 μg/mL suppressed pro-inflammatory cytokine production and inhibited JAK1/STAT3 signaling. In DSS-induced colitis in mice, AED at 600 mg/kg for 7 days mitigated DSS-induced colonic injury, restored tight junction proteins, and improved epithelial barrier integrity. Integrated transcriptomic and metabolomic analyses identified AED-associated alterations in arginine-polyamine and taurine-hypotaurine metabolism, while network pharmacology and molecular docking suggested angiotensin-converting enzyme (ACE) and von Willebrand factor (VWF) as candidate functional targets for further investigation. Collectively, these findings indicate that AED exerts anti-colitis effects in association with coordinated changes in inflammatory signaling, metabolic pathways, and barrier-related markers, supporting its potential as a food-derived functional ingredient candidate for ulcerative colitis management. Full article
(This article belongs to the Section Nutraceuticals, Functional Foods, and Novel Foods)
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25 pages, 2699 KB  
Review
Produced Water from Oil and Gas Operations in Agronomic and Forage Crop Production: A Review of Implications, Opportunities, and Risks
by Bishnu Ghimire, Caitlyn Cooper, S. V. Krishna Jagadish and Aaron Norris
Sustainability 2026, 18(11), 5283; https://doi.org/10.3390/su18115283 (registering DOI) - 25 May 2026
Abstract
Water scarcity has become a major challenge for agriculture, particularly in arid and semi-arid regions where irrigation is essential for sustaining crop and forage production. As freshwater supplies face growing pressure from climate change, urban growth, and industrial use, there is increasing interest [...] Read more.
Water scarcity has become a major challenge for agriculture, particularly in arid and semi-arid regions where irrigation is essential for sustaining crop and forage production. As freshwater supplies face growing pressure from climate change, urban growth, and industrial use, there is increasing interest in exploring alternative water sources to support sustainable agriculture. Produced water, a byproduct of oil and gas extraction, may represent an alternative water source in water-limited regions like the southwestern United States and the Middle East. However, raw produced water often contains high levels of salinity, trace metals, hydrocarbons, and naturally occurring radioactive materials, which cause risks to soils, crops, livestock, and food systems. This review synthesizes peer-reviewed studies up to January 2026 and reports on the agricultural application of treated produced water, focusing on its effects on soil properties, crop growth, yield, and forage nutritive quality. Existing research shows that treated produced water could be used for grain as well as forage crops under controlled conditions, but poorly treated and managed applications can lead to increases in soil salinity, structural degradation, reduced nutrient uptake, and hindered crop performance. In forage systems, irrigation with treated produced water has also been associated with changes in nutritive value, increasing concerns for livestock health. Several knowledge gaps remain, including limited long-term field studies, insufficient information on crop-specific contaminant thresholds, incomplete assessment of treatment and remediation strategies under different environmental conditions, and the absence of a consistent framework for classifying the chemistry of treated produced water for agricultural applications. Addressing these gaps through integrated soil, crop, and water research and the development of clear policies and guidelines is essential for determining whether treated produced water can be safely and sustainably used in agriculture under growing water scarcity. Full article
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14 pages, 2116 KB  
Article
Defect-Tolerant Interfacial Compatibilization of Heterogeneous Recycled Polypropylene via Binary iPP-g-MA/aPP-g-MA Masterbatches
by Ruohan Liu, Haidi Cai, Zhonghua Tang and Liang Tong
Appl. Sci. 2026, 16(11), 5266; https://doi.org/10.3390/app16115266 - 25 May 2026
Abstract
Single-use polypropylene (PP) food containers represent a rapidly growing waste stream characterized by compositional heterogeneity and microstructural defects. Conventional reactive compatibilization using isotactic maleic anhydride-grafted PP (iPP-g-MA) provides rigid crystalline anchoring but lacks the interfacial flexibility to accommodate complex micro-defects. Herein, [...] Read more.
Single-use polypropylene (PP) food containers represent a rapidly growing waste stream characterized by compositional heterogeneity and microstructural defects. Conventional reactive compatibilization using isotactic maleic anhydride-grafted PP (iPP-g-MA) provides rigid crystalline anchoring but lacks the interfacial flexibility to accommodate complex micro-defects. Herein, we propose a defect-tolerant compatibilization strategy by developing a binary iPP-g-MA/aPP-g-MA masterbatch for real post-consumer rPP derived from food-service containers. The amorphous aPP-g-MA component is proposed to provide a compliant interfacial environment that accommodates stress concentrations associated with microscale defects, whereas the iPP-g-MA component contributes crystalline anchoring with the recycled PP matrix. This soft/hard interfacial architecture is supported by grafting-degree analysis, GPC, XRD, DSC crystallization behavior, and SEM fracture morphology. The 1:1 mass-ratio binary formulation shows a marked improvement in elongation at break to 200%, representing a 203% increase relative to the single-component iMA system. The notched Charpy impact strength is enhanced to 8.98 kJ m−2, while tensile strength is retained at 20.9 MPa within the typical strength–ductility trade-off of polymer toughening. TGA shows no premature degradation within the melt-processing window, indicating adequate thermal stability for melt reprocessing. This study provides a compositionally tunable, data-supported route for high-value upcycling of heterogeneous post-consumer polyolefins. From an application viewpoint, the improved ductility-impact balance makes the material relevant to injection-moulded semi-structural products such as storage crates, appliance housings, and automotive interior panels. Full article
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5 pages, 152 KB  
Editorial
Dietary Supplementation in Aquaculture
by Neculai Patriche and Elena Sîrbu
Fishes 2026, 11(6), 316; https://doi.org/10.3390/fishes11060316 - 25 May 2026
Abstract
Sustainability, one of the most important areas of development in the aquaculture sector, aims to ensure the efficient production of high-protein food with minimal environmental impact [...] Full article
(This article belongs to the Special Issue Dietary Supplementation in Aquaculture)
21 pages, 1517 KB  
Article
An Exploration of Aquatic Food Production and Marketing Mix in the Coastal States of Nigeria
by Shehu L. Akintola, Lateef A. Badmos, Akinkunmi S. Ojo, Gbenga R. Ajepe, Matthew A. Ajibade, Mary A. Gbadamosi, Victor T. Okomoda, Idowu J. Fasakin, Sunil Siriwardena, Charles Iyangbe, Esther W. Magondu and Rodrigue Yossa
Aquac. J. 2026, 6(2), 18; https://doi.org/10.3390/aquacj6020018 - 25 May 2026
Abstract
This field study examined extant aquatic food production and marketing in the three coastal states of Lagos, Ogun, and Ondo before IMTA across 15 Local Government Areas (LGAs)/Local Council Development Areas (LCDAs). Marketing mix practices in coastal aquatic food systems were explored through [...] Read more.
This field study examined extant aquatic food production and marketing in the three coastal states of Lagos, Ogun, and Ondo before IMTA across 15 Local Government Areas (LGAs)/Local Council Development Areas (LCDAs). Marketing mix practices in coastal aquatic food systems were explored through a structured, qualitative assessment using a multi-value chain perspective. Monthly sales volumes most frequently fell within the range of 1–5 tonnes. The local market was dominant, with some sales in the international markets. Respondents asserted that post-harvest processing was diverse, and some were satisfied with the technology available to preserve their products. Cold storage practices across coastal states were hindered by unreliable power supply. Zero-level channel distribution dominated among traders, with over 90% relying on word-of-mouth (WOM) to promote their products. Consumers showed a strong preference for the quality of local products and expressed openness to incorporating seaweed into their purchases. Health benefits, taste, and other reasons for purchase decisions varied significantly across the state χ2 (df = 8, n = 300) = 92.39, p < 0.001. These findings provide a baseline for IMTA in Nigeria, highlighting existing strengths, market dynamics, and infrastructure gaps that must be addressed to support sustainable integration. Full article
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15 pages, 645 KB  
Article
Compositional Characterization of Milk Thistle (Silybum marianum) Seeds and Press Cake with Emphasis on Press Cake Valorization
by Sina Makouie, Jolanta Małajowicz, Agata Gόrska, Iga Piasecka-Lenartowicz, Barbara Strojny-Cieślak, Michał Pruchniewski, Bartłomiej Zieniuk and Joanna Bryś
Appl. Sci. 2026, 16(11), 5265; https://doi.org/10.3390/app16115265 - 24 May 2026
Abstract
Milk thistle (Silybum marianum) oil production generates substantial quantities of seed cake, an underutilized by-product with potential as a source of nutrients and bioactive compounds. This study aimed to characterize milk thistle cakes from two industrial sources (MTC1 and MTC2) and [...] Read more.
Milk thistle (Silybum marianum) oil production generates substantial quantities of seed cake, an underutilized by-product with potential as a source of nutrients and bioactive compounds. This study aimed to characterize milk thistle cakes from two industrial sources (MTC1 and MTC2) and their corresponding seeds (MTS1 and MTS2), focusing on compositional properties, fatty acid profile, and antioxidant activity assessed using the DPPH scavenging assay. Proximate analysis showed that the cakes retained significant residual oil (9.26–14.51 g 100 g−1) and protein (16–19 g 100 g−1), with low water activity (<0.33), indicating good storage stability. Fatty acid analysis revealed a predominance of polyunsaturated fatty acids (49–52%), mainly linoleic acid (C18:2 n-6), confirming their nutritional value. Differences between industrial sources indicated variability associated with raw material and processing conditions. Extraction solvent significantly affected bioactive compound recovery from the oil fraction. Dichloromethane extracts exhibited higher total phenolic content (up to 8.87 mg GAE g−1) and stronger DPPH radical scavenging activity (up to 28.07%) compared to hexane extracts, which may be attributed to a greater extraction of moderately polar phenolic compounds, including flavonolignan-type constituents potentially associated with silymarin complex. Overall, milk thistle cake represents a promising raw material for the recovery of natural antioxidants and valuable lipids, supporting its application in functional food or feed products and sustainable biorefinery processes. Full article
(This article belongs to the Special Issue Advancements in Food Nutrition and Bioactive Compounds)
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23 pages, 1965 KB  
Review
Frozen Foods: A Comprehensive Review of Technologies, Challenges, and Future Prospects
by Hongjuan Teng, Yuejiao Xing, Yue San, Li Zheng, Zhongjiang Wang and Bailiang Li
Processes 2026, 14(11), 1703; https://doi.org/10.3390/pr14111703 - 24 May 2026
Abstract
Freezing effectively extends the shelf life of food and maintains product quality by inhibiting microorganisms, enzyme activity, and chemical reactions. However, issues such as ice crystal formation, protein denaturation, lipid oxidation, and the low-temperature adaptability of psychrophilic microorganisms during the freezing process can [...] Read more.
Freezing effectively extends the shelf life of food and maintains product quality by inhibiting microorganisms, enzyme activity, and chemical reactions. However, issues such as ice crystal formation, protein denaturation, lipid oxidation, and the low-temperature adaptability of psychrophilic microorganisms during the freezing process can directly affect the final quality of frozen foods. Among these, the size and distribution of ice crystals are key factors determining the extent of tissue damage. Therefore, this review aims to identify innovative and optimized freezing and frozen storage strategies. In order to save energy and improve product quality, various new technologies have emerged in recent years, such as ultrasonic-assisted freezing, high-pressure freezing, and magnetic-field-assisted freezing. This study systematically discusses the principles, applications, and impact mechanisms of these technologies on frozen foods. Furthermore, this study proposes the future development trends of frozen foods, filling the gap in the current food industry where there is a lack of systematic discussion and evaluation of frozen foods. It provides technical support and research directions for continuous development and innovation in the field of frozen foods. Full article
(This article belongs to the Section Food Process Engineering)
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24 pages, 5093 KB  
Article
Scale-Up Green Synthesis of Maghemite–Citrus reticulata Hybrid Nanoparticles with High Magnetization and Their Effects on Cd/Ni Uptake in Cacao Seedlings
by Juan A. Ramos-Guivar, Mercedes del Pilar Marcos-Carrillo, Melissa-Alisson Mejía-Barraza, Renzo Rueda-Vellasmin, Noemi-Raquel Checca-Huaman, Edson Caetano Passamani, Cesar Oswaldo Arévalo-Hernández and Enrique Arévalo-Gardini
Agriculture 2026, 16(11), 1151; https://doi.org/10.3390/agriculture16111151 - 24 May 2026
Abstract
Metal accumulation in cacao (Theobroma cacao L.) cultivation represents an important agronomic and food-safety concern, particularly in acidic tropical soils where cadmium (Cd) and other trace metals can become bioavailable and translocate to plant tissues. Green magnetic nanomaterials offer a potential strategy [...] Read more.
Metal accumulation in cacao (Theobroma cacao L.) cultivation represents an important agronomic and food-safety concern, particularly in acidic tropical soils where cadmium (Cd) and other trace metals can become bioavailable and translocate to plant tissues. Green magnetic nanomaterials offer a potential strategy for reducing metal mobility in agricultural substrates, but their performance depends on surface chemistry, dose, and plant genotype. In this study, we synthesized and evaluated MCRES, defined here as a maghemite–Citrus reticulata extract system, a biofunctionalized γ-Fe2O3-based nanosystem prepared by coupling iron oxide nanoparticles (NPs) with a 3% (w/v) Citrus reticulata peel extract. The objective was to determine whether citrus-mediated biofunctionalization could produce a scalable magnetic nanoamendment capable of modifying Cd and naturally occurring Ni partitioning in cacao seedlings. MCRES was recovered magnetically and dried, yielding 8.44 g of product from 10 g of precursor. Rietveld analysis performed in X ray diffractograms confirmed phase-pure cubic γ-Fe2O3 with a lattice parameter of 0.8332 nm, a crystallite size of 11.3(1) nm, and satisfactory refinement quality (χ2 ≈ 1.34). Transmission electron microscope images showed quasi-spherical NPs with a log-normal size distribution centered at 7.5 nm. Magnetic measurements showed superparamagnetic-like behavior at 300 K, high saturation magnetization values of 62 emu g−1 at 300 K and 71 emu g−1 at 5 K, and elevated effective anisotropy values obtained from the Law of Approach to Saturation fitting. MCRES was applied at 0, 1, 2, 4, and 6 g pot−1 to cacao seedlings containing Cd-amended Ultisol with naturally occurring Ni. Plant responses were genotype and dose dependent: TSH-1188 genotype showed limited dose sensitivity for most biometric variables, whereas ICS-95 genotype showed significant dose effects, with maximum growth at the 2 g pot−1 treatment. Metal-partitioning results indicated that Cd remained comparatively mobile toward shoots, whereas Ni was preferentially retained in roots. In TSH-1188 genotype, the Ni translocation factor decreased from 3.07 in the control to 0.85–1.00 at higher MCRES doses. Compared with previous work on non-biofunctionalized nanomaghemite, these results suggest that citrus-mediated biofunctionalization produces a distinct Cd/Ni partitioning response. Overall, MCRES is recommended as a promising nursery-scale green nanoamendment for reducing metal mobility in cacao cultivation, but its agronomic use should be optimized according to genotype and dose. Future work should include side-by-side comparisons with unfunctionalized γ-Fe2O3, Citrus reticulata extract alone, and non-contaminated controls under field conditions to validate its long-term effectiveness and environmental safety. Full article
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