Search Results (735)

Cultivation practices such as fruit thinning and soil management with ground covers are commonly applied in Citrus orchards, yet their physiological impact on young trees remains poorly documented. This study evaluated the effects of manual fruit thinning and weed-control mesh on vegetative growth, fruit development, and leaf mineral composition of Citrus sinensis L. Osbeck cv. ‘Navelina’ grafted on Citrus macrophylla. A six-month field experiment was conducted in southeastern Spain under semi-arid Mediterranean conditions using six treatments that combined different soil coverage and subsurface drainage systems. After physiological fruit drop, trees were standardized to ten fruits per plant. Vegetative parameters (canopy and trunk dimensions), fruit growth (size, juice content), and foliar nutrient concentrations were monitored. Trees with ground cover showed significantly greater canopy expansion and juice yield compared to uncovered controls. A negative correlation between fruit number and canopy-to-fruit volume ratio highlighted the trade-off between vegetative vigor and fruit load. Foliar analysis revealed lower micronutrient concentrations (Fe, Mn, B, Zn) in uncovered trees, suggesting reduced nutritional status. These findings demonstrate that combining early thinning with weed-control mesh promotes vegetative vigor, improves juice yield, and enhances nutrient uptake, providing practical insights for optimizing orchard establishment and early Citrus productivity in water-limited environments. Full article

4-Chlorophenoxyacetic acid (4-CPA) is an auxin-type plant growth regulator widely used in fruit and vegetable production. However, its influence on the nutritional and sensory qualities of horticultural crops remains insufficiently characterized. This study investigated the influence of 4-CPA application and oscillator-mediated pollination on the metabolic composition of fully ripe fruits of Solanum lycopersicum var. cerasiforme cv. ‘Zheyingfen No. 1’. Two concentrations of 4-CPA (16 mg/L and 8 mg/L) were applied during flowering, and their effects on amino acids, soluble sugars, organic acids, and volatile compounds (VOCs) were comparatively analyzed. The results indicated that treatment with 8 mg/L 4-CPA treatment significantly increased the total amino acid content in ripe fruits compared with the control and the 16 mg/L treatment. Among the 17 amino acids identified, the contents of umami-related amino acids, including glutamic acid (Glu) and aspartic acid (Asp), were markedly enhanced. In particular, Glu content in the C8 treatment was the highest and accounted for more than 50% of the total amino acid content. The accumulation of sugars was not significantly affected by 4-CPA treatment, while the C8 treatment resulted in the lowest level of total organic acids, which are crucial for flavor development at the ripening stage. A 29.35% increase in VOCs was observed” for conciseness in 4-CPA-treated fruits compared with the control. Analysis of relative odor activity values (rOAVs) showed that although 4-CPA treatment reduced the number of aroma-active compounds, it promoted the accumulation of β-ionone, thereby shifting the tomato fruit aroma profile toward floral, woody, sweet, and fruity notes. In summary, 4-CPA treatment regulated the nutritional and flavor quality of ripe cherry tomato fruits by increasing the content of Glu and other amino acids, enhancing the diversity of VOCs, and promoting the formation of key aroma-active substances such as β-ionone. Full article

The utilization of proper fermentation techniques is a widely recognized, efficacious approach in animal husbandry for enhancing the feed quality. However, research on vegetable waste, particularly that of roots, stems, leaves, fruits, and peels, has been rarely reported. To this end, the present study was carried out to examine the impact of vegetable leaf fermentation on growth performance, immune function, antioxidant levels, intestinal morphology, and microbial composition in sheep. Fifty-four male sheep (Oula) with an average age of 6 months and an average body weight of (21.53 ± 2.03) kg were randomly divided into three treatment groups, with six replicates each. The groups were fed with a basal diet (CON), 30% commercial fermented concentrate (CFC), and 30% vegetable leaf fermented concentrate (VFC). The results showed that compared to the CON group, both the commercial fermented concentrate and the vegetable leaf fermented concentrate improved the final weight (8.93%), average daily gain (30.67%), and dry matter intake of the sheep (1.62%). VFC increased the serum T-AOC (34.45%) and significantly increased the activities of serum and liver GSH-PX (10.95%). Meanwhile, the addition of vegetable leaf fermented concentrate increased the levels of serum IgA (63.21%), IgG (73.06%), and IgM (69.41%). VFC increased the villus height of the jejunum by 87.4% and the ileum by 185.5% and improved the villus height/crypt depth (V/C) ratio of the duodenum and ileum. CFC can also increase the villus height of the duodenum and jejunum, but has no effect on the morphology of the ileum. In addition to its other regulatory effects, VFC can further improve the richness and diversity of the rumen microbial community in sheep, with a notable enhancement in the relative abundance of key phyla, including Bacteroidetes, Ascomycota, Zygomycota, Chytridiomycota, and Basidiomycota. At the same time, the relative abundance of Succinivibrio was reduced. It can thus be concluded that the vegetable leaf fermented concentrate improves the growth performance and intestinal health of sheep. Full article

Carboxymethyl chitosan (CMCS) is ideal for active packaging due to its non-toxicity and degradability, but its poor film-forming performance (strong hydrophilicity, weak mechanical properties, and low antibacterial activity) limits practical use. This study prepared a new edible antibacterial presFervation film (SDEWL-CMCS) by adding salted duck egg white lysozyme (SDEWL) to CMCS (as the film-forming substrate). It investigated how SDEWL concentration affects the composite film’s properties (thickness, water solubility, moisture/oil resistance, mechanical properties, and antibacterial activity) and tested the film’s preservation effect on small nectarines. The results showed the composite film had significantly improved packaging and antibacterial properties: compared to pure CMCS film, it had higher tensile strength, lower water solubility, better oil resistance and water vapor barrier performance, and stronger antibacterial activity against Escherichia coli and Staphylococcus aureus (larger inhibition zone diameters). The SDEWL-CMCS film effectively preserved small nectarines by inhibiting surface bacteria, regulating the preservation environment, and delaying fungal decay. This study confirms the film’s potential as a sustainable fruit packaging alternative, providing a theoretical basis for developing new fruit/vegetable preservation packaging and reducing the food industry’s reliance on non-degradable petroleum-based packaging. Full article

The transmission of Escherichia coli through contaminated fruits and vegetables poses serious public health risks and has led to several national outbreaks in the USA. To enhance food safety, rapid and reliable detection of E. coli on produce is essential. This study evaluated the performance of the CSI-D+ system combined with deep learning for detecting varying concentrations of E. coli on citrus and spinach leaves. Eight levels of E. coli contamination, ranging from 0 to 10⁸ colony-forming units (CFU)/mL, were inoculated onto the leaf surfaces. For each concentration level, 10 droplets were applied to 8 citrus and 12 spinach leaf samples (2 cm in diameter), and fluorescence images were captured. The images were then subdivided into quadrants, and several post-processing operations were applied to generate the final dataset, ensuring that each sample contained at least 2–3 droplets. Using this dataset, multiple deep learning (DL) models, including EfficientNetB7, ConvNeXtBase, and five YOLO11 variants (n, s, m, l, x), were trained to classify E. coli concentration levels. Additionally, Eigen-CAM heatmaps were used to visualize the spatial responses of the models to bacterial presence. All YOLO11 models outperformed EfficientNetB7 and ConvNeXtBase. In particular, YOLO11s-cls was identified as the best-performing model, achieving average validation accuracies of 88.43% (citrus) and 92.03% (spinach), and average test accuracies of 85.93% (citrus) and 92.00% (spinach) at a 0.5 confidence threshold. This model demonstrated an inference speed of 0.011 s per image with a size of 11 MB. These findings indicate that fluorescence-based imaging combined with deep learning for rapid E. coli detection could support timely interventions to prevent contaminated produce from reaching consumers. Full article

Background/Objectives: Vitamin C plays a vital role in human health, functioning as a powerful antioxidant and enzymatic cofactor. Although vitamin C bioavailability from food versus supplements has been debated, few studies have examined how intake form affects absorption and physiological markers. Methods: This randomized, controlled, crossover trial aimed to compare the bioavailability of vitamin C consumed as a supplement, through raw fruits and vegetables, or through fruit and vegetable juice. Twelve healthy adults underwent three 1-day crossover trials, each separated by a 2-week washout. Participants consumed 101.7 mg of vitamin C via powder, raw fruits and vegetables (186.8 g), or juice (200 mL). Plasma and urinary vitamin C concentrations, urinary metabolites (¹H NMR), and antioxidant activity (ORAC and TRAP) were assessed over 24 h. Results: All interventions elevated plasma vitamin C levels, with juice yielding the highest AUC (25.3 ± 3.2 mg/dL·h). Urinary vitamin C increased in all groups. Metabolomics revealed increased urinary excretion of mannitol, glycine, taurine, dimethylglycine (DMG), and asparagine, and decreased choline and dimethylamine (DMA). Notably, urinary mannitol increased only in the morning. Choline significantly decreased after powder intake (p = 0.001), with similar trends observed in the other groups. DMG and glycine increased following raw and juiced vegetable intake. Antioxidant activity showed transient ORAC elevation post-powder but no sustained improvements. Conclusions: Vitamin C is bioavailable from all intake forms, with juice providing the most efficient absorption. Urinary metabolite changes suggest microbiota-related modulation, while antioxidant activity improvements were limited. Full article

Nitrogen (N) deficiency in apples (Malus × domestica Borkh.) leads to characteristic physiological symptoms, including leaf and fruit discoloration. Fertigation, i.e., the application of dissolved fertilizers, can significantly improve the growth and fruit quality of apples while optimizing nutrient uptake through a more precise and better timed application than conventional fertilization. This study therefore investigates how different fertilization treatments affect the N concentration of different age categories of apple leaves. Apples of the variety ‘Braeburn’ were grown hydroponically on the low-vigorous rootstock M9. Four fertilizer treatments were used: (1) Hoagland solution (HS); (2) HS nitrogen excluded; (3) HS iron excluded; and (4) HS magnesium excluded. Through vegetation, leaf samples were taken from three shoot positions representing different leaf ages (young, semi-young and old) and then chemically analyzed. The lowest N concentrations across all leaf ages and sampling moments were found in the treatment with N excluded (1.69–2.07% N), while the highest values occurred in the treatments where iron (2.00–2.49% N) or magnesium (1.98–2.37% N) were excluded. The seasonal changes in N concentration reflect interactions between the leaf age and the sampling moment. These results show that the N concentration of apple leaves strongly depends on the type of fertilization. Full article

Adequate intake of fruits and vegetables (F + V) supports healthy growth and development in children, yet many New Zealand children do not meet national dietary recommendations, and methods to evaluate intake require good reliability. Objectives: To establish the validity and reliability of a modified 24 h multiple pass recall (MPR) for evaluating F + V and carotenoid intakes in children aged 9–13 years. The reliability of the Veggie Meter^® (VM^®), a non-invasive reflection spectrometer to estimate skin carotenoid scores and derive blood carotenoid concentrations, was also examined. Methods: Thirty-two children (20 boys, 12 girls) completed three 24 h MPRs and parent-assisted weighed food diaries (WFDs) on randomised weekdays and weekends. Skin carotenoid scores were assessed using the VM^®. The validity of the MPR was evaluated against WFDs using log-transformed Pearson correlations and mean x-axis bias. The reliability was assessed by the coefficient of variation (CV) and Pearson correlations. Results: Participants did not meet recommended F + V intakes (5–5.5 servings/day): MPR (mean fruit 1.3 servings/day; vegetables 2.0), WFD (fruit 1.3; vegetables 1.9). The MPR was a valid tool to estimate fruit and vegetable daily servings (combined-day Pearson coefficients > 0.71) with only trivial–small standardized mean bias-offset vs. WFD; however, the reliability was poor for the MPR-estimated carotenoid intake (CV 126%) and F + V intake. In contrast, the VM^® was reliable (Pearson correlation 0.97–0.99) with low measurement error (CV 4.0–5.2%). Conclusions: The modified 24 h MPR was valid but unreliable for estimating F + V and carotenoid intake. The VM^® demonstrated high reliability as a biomarker of skin carotenoid status in children. Full article

Pineapple translucency is a major physiological disorder in ‘Tainong 17’ (Golden Diamond) that severely impairs fruit quality, storability, and market value, yet its physiological basis remains poorly understood. To clarify the underlying mechanisms, we conducted two seasons of field surveys across 24 plots in eleven pineapple orchards in Hainan, China, comparing translucent and healthy fruits in terms of plant growth, nutrient status, fruit quality, cell wall composition, and soil properties. Our results showed that translucency significantly reduced fruit quality, with soluble solids and ascorbic acid contents decreasing by 9.7% and 16.3%, respectively. Translucent plants exhibited markedly increased biomass, whereas fruit dry matter was reduced by 21.6%. In addition, affected plants accumulated 40–70% more nitrogen in leaves, stems, and fruits, accompanied by 23% and 14% reductions in abscisic acid concentrations in leaves and fruits, respectively. Calcium and boron allocation to fruits was impaired, with fruit Ca and B contents decreasing by 25.1% and 50.4%, respectively, despite increased levels in vegetative organs. These nutrient imbalances coincided with a 16.4% decrease in protopectin, a 5.3% decrease in cellulose, and a 15.5% increase in soluble pectin, indicating cell-wall loosening. Collectively, our findings demonstrate that excessive nitrogen input disrupts carbon–nitrogen metabolism and ABA signaling, elevates fruit N/Ca ratios, and accelerates cell-wall remodeling, thereby predisposing fruits to translucency, particularly under humid or rainy conditions. Full article

Nutritional factors play a crucial role in many gynecological disorders, particularly those influenced by estrogen. Uterine fibroids are benign tumors that affect a large proportion of women of reproductive age, especially between 30 and 40 years. These lesions may cause significant symptoms, including pelvic pain, heavy menstrual bleeding, and infertility. In younger women, the onset of fibroids is often associated with familial and genetic predisposition, whereas in adulthood, hormonal influences linked to environmental factors and states of exogenous or endogenous hyperestrogenism are more frequently observed. In both contexts, supportive management through an appropriate diet may provide clinical benefit. Although the precise pathogenesis remains incompletely understood, hormonal, genetic, and environmental components—particularly hyperestrogenism—are considered key contributors to fibroid development. Current evidence suggests that consumption of saturated fats, particularly from red meat and full-fat dairy, may raise circulating estrogen concentrations and contribute to the development of fibroids. In contrast, diets abundant in fiber, fruits, and vegetables appear to exert a protective effect, potentially lowering fibroid risk. Obesity, through increased aromatization and consequent estrogen production, also represents an established risk factor. This narrative review aims to explore the role of nutritional determinants in the onset and progression of uterine fibroids, with a specific focus on the impact of individual nutrients, foods, and dietary patterns on clinical outcomes. Particular emphasis is placed on obesity and macronutrient composition (e.g., high-fat versus high-fiber dietary regimens) as potential modulators of circulating estrogen levels and, consequently, fibroid growth dynamics. Furthermore, the potential of nutritional strategies as complementary therapeutic approaches, capable of integrating established clinical practices, is examined. Full article

Background: Tyrosinase is a copper-dependent oxyreductase capable of catalyzing the oxidation of mono- and diphenols. Its activity is crucial in the biosynthetic pathway of melanin, the pigment responsible for the pigmentation of mammalian skin and fur, and protecting their skin from harmful UV radiation. Overproduction of this pigment leads to numerous pathological conditions, including the most severe form of skin cancer—malignant melanoma. Furthermore, tyrosinase produced in plant tissues leads to the browning of damaged vegetables and fruits. Therefore, the search for compounds that effectively and efficiently control tyrosinase activity is desirable for both pharmaceutical and food applications. Methods: A group of six boronate derivatives of thiosemicarbazones was synthesized, and their inhibitory properties against tyrosinase were determined. Furthermore, their ability to inhibit melanogenesis and proliferation in SK-MEL-3 and Hs294T cells was investigated. Docking simulations were performed to determine the nature of the inhibitor–protein interactions. Results: The tested inhibitors exhibited half-maximal inhibitory concentrations (IC₅₀) in the micromolar range. The best inhibitor, compound 6, had an IC₅₀ of 1.4 µM. The tested compounds exhibited poor selectivity for cell lines capable of high and low tyrosinase overexpression, with inhibitor 4 proving to be the most selective compound among those tested. Molecular modeling results indicate that the compounds with the highest activity against tyrosinase interact with the active cavity and the copper ions present within it via a boron moiety substituted on the aromatic ring of the thiosemicarbazones. Cell-based experiments indicated limited antiproliferative effects up to 100 µM across the tested lines. The compounds demonstrated weak antiproliferative effects in SK-MEL-3 and Hs-294T up to 100 µM. Conclusions: Our results show that the introduction of a boronic acid moiety is an alternative to carboxylic acid derivatives, improving the inhibitory activity of boron analogs (by fourfold) against fungal tyrosinase. Full article

In the shift toward sustainable energy, there is a strong demand for efficient and durable energy storage solutions. Supercapacitors, in particular, are a promising technology, but they require high-performance materials that can be produced using simple, eco-friendly methods. This has led researchers to investigate new materials and composites that can deliver high energy and power densities, along with long-term stability. Herein, we report a green synthesis approach to create a composite material consisting of reduced graphene oxide and silver nanoparticles (rGO/Ag). The method uses ascorbic acid, a natural compound found in fruits and vegetables, as a non-toxic agent to simultaneously reduce graphene oxide and silver nitrate. To enhance electrochemical performance, the incorporation of silver nanoparticles into the rGO structures is optimized. In this study, different molar concentrations of silver nitrate (1.0, 0.10, and 0.01 M) are used to control silver nanoparticle loading during the synthesis and reduction process. A correlation between silver concentration, defect density in rGO, and the resulting capacitive behavior was assessed by systematically varying the silver molarity. The synthesized materials exhibited excellent performance as supercapacitor electrodes in a three-electrode configuration, with the rGO/Ag 1.0 M composite showing the best performance, reaching a maximum specific capacitance of 392 Fg⁻¹ at 5 mVs⁻¹. Furthermore, the performance of this optimized electrode material was investigated in a two-electrode configuration as a coin cell device, which demonstrates a maximum areal-specific capacitance of 22.63 mFcm⁻² and a gravimetric capacitance of 19.00 Fg⁻¹, which is within the range of commercially viable devices and a significant enhancement, outperforming low-level graphene-based devices. Full article

Natural preservatives are gaining attention as chemical-free solutions to extend produce shelf life and prevent microbial spoilage. Therefore, sweet potato peel (SPP) was investigated as a source of antifungal bioactive compounds in this study. We evaluated essential oils and, for the first time, a bound terpene (BT) concentrate extracted from SPP against Aspergillus flavus, using both in vitro and in vivo assays. Murasaki organic Japanese sweet potato (Ipomoea batatas L.) peels, A. flavus AF13, a highly aflatoxigenic fungus, and Creole tomato (Solanum lycopersicum) fruits were used in the study. Essential oils were extracted by hydrodistillation (HD) and vacuum distillation (VD), while the BT fraction was isolated and concentrated. HD and VD yielded 19 and 10 terpenes, respectively, with linalool and α-terpineol dominating and representing more than 50% of total terpenes in both distillates. The BT concentrate demonstrated significant inhibition of A. flavus growth at concentrations starting from 12.5 µL/mL. The strongest effect was observed at 100 µL/mL, with a 26.0 ± 1.0 mm inhibition zone and 55.56 ± 4.53% growth reduction. In contrast, HD and VD distillates showed no antifungal activity in either in vitro or in vivo assays. Consistently, the BT concentrate-treated tomatoes reduced fungal growth and spoilage, with lesion diameters less than 10 mm after 7 days of storage, while the HD and VD distillate treatments had diameters over 20 mm, and the untreated control had diameters over 60 mm. These findings highlight that SPP waste could be an economical and bio-based source for developing natural antifungal ingredients. The success is anticipated to offer a potential alternative to current synthetic fungicides in preventing fungi A. flavus-induced spoilage of nightshade vegetables. Full article

Introduction: Metabolic syndrome (MetS), a clustering of cardiovascular disease (CVD) risk factors, is associated with increased mortality. Fruit and vegetable (FV) intake is inversely associated with CVD risk, and carotenoids, bioactive compounds found in brightly colored FVs, can be measured in serum and skin as biomarkers of intake. While serum and skin carotenoids are correlated in healthy populations, this relationship is not well understood in older adults with MetS, who may have altered carotenoid absorption or metabolism. Methods: In this cross-sectional study, adults aged 55+ were assessed for serum carotenoid concentrations, pressure-mediated reflection spectroscopy (RS) skin carotenoid scores, self-reported FV intake, sociodemographic characteristics, and comorbidities. MetS status was determined using the National Cholesterol Education Program Adult Treatment Panel III criteria (77 with MetS, 63 without). Linear regression models evaluated group differences in carotenoid levels. Associations between serum and skin carotenoids were examined using Spearman correlation and multivariable regression. Results: Participants with MetS had significantly lower serum alpha-carotene (52%), beta-carotene (39%), and total carotenoids (22%) than those without MetS (all p < 0.002). Differences remained after adjustment for sociodemographic and health-related factors. No significant group differences were found for lycopene, lutein, cryptoxanthin, or skin carotenoid scores. Total serum carotenoids were positively correlated with skin scores (r = 0.58, p < 0.001), and this association persisted in adjusted models. Conclusions: Older adults with MetS had lower serum carotenoid levels, primarily due to alpha- and beta-carotene. This serum–skin correlation supports RS-based skin measurement as a practical, non-invasive assessment of carotenoid status. Full article

Dark fermentation (DF) has gained increasing interest over the past two decades as a sustainable route for biohydrogen production; however, understanding how reproducible the process can be, both from macro- and microbiological perspectives, remains limited. This study assessed the reproducibility of a parallel continuous DF system using fruit-vegetable waste as a substrate under strictly controlled operational conditions. Three stirred-tank reactors were operated in parallel for 90 days, monitoring key process performance indicators. In addition to baseline operation, different process enhancement strategies were tested, including bioaugmentation, supplementation with nutrients and/or additional fermentable carbohydrates, and modification of key operational parameters such as pH and hydraulic retention time, all widely used in the field to improve DF performance. Microbial community structure was also analyzed to evaluate its reproducibility and potential relationship with process performance and metabolic patterns. Under these conditions, key performance indicators and core microbial features were reproducible to a large extent, yet full consistency across reactors was not achieved. During operation, unforeseen operational issues such as feed line clogging, pH control failures, and mixing interruptions were encountered. Despite these disturbances, the system maintained an average hydrogen productivity of 3.2 NL H₂/L-d, with peak values exceeding 6 NL H₂/L-d under optimal conditions. The dominant microbial core included Bacteroides, Lactobacillus, Veillonella, Enterococcus, Eubacterium, and Clostridium, though their relative abundances varied notably over time and between reactors. An inverse correlation was observed between lactate concentration in the fermentation broth and the amount of hydrogen produced, suggesting it can serve as a precursor for hydrogen. Overall, the findings presented here demonstrate that DF processes can be resilient and broadly reproducible. However, they also emphasize the sensitivity of these processes to operational disturbances and microbial shifts. This underscores the necessity for refined control strategies and further systematic research to translate these insights into stable, high-performance real-world systems. Full article