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17 pages, 1521 KB  
Article
Morphological Advantages of Nano-Zinc: Effects on Yield and Quality Improvement in Blue Honeysuckle
by Xuefei Ji, Wei Li, Yuxi Chen, Haihui She, Shan Wang, Chunshuang Li, Hao Sun and Junwei Huo
Plants 2026, 15(10), 1520; https://doi.org/10.3390/plants15101520 - 15 May 2026
Viewed by 186
Abstract
Blue honeysuckle (Lonicera caerulea L.) is subject to environmental stressors, leading to variability in both severe fruit drop during development and fruit quality. Zinc, an essential micronutrient, is critical to sustainable fruit tree production by enhancing yield and nutritional quality. Different forms [...] Read more.
Blue honeysuckle (Lonicera caerulea L.) is subject to environmental stressors, leading to variability in both severe fruit drop during development and fruit quality. Zinc, an essential micronutrient, is critical to sustainable fruit tree production by enhancing yield and nutritional quality. Different forms of zinc fertilizers, particularly nano-zinc versus conventional ionic zinc, exhibit marked differences in absorption efficiency and agronomic performance, thereby determining their practical efficacy. In this two-year study, we evaluated the effects of foliar-applied zinc forms, ZnO nanoparticles (30, 50, and 90 nm) and ionic zinc (ZnCl2 and ZnSO4), applied at the young fruit, veraison, and maturity stages on yield and fruit quality. Results showed that ZnO nanoparticles were more effective than ionic zinc at 80 mg/L. In particular, among the ZnO NP treatments, 90 nm ZnO NPs exhibited the best overall effect, significantly improving fruit quality. The 30 nm ZnO NPs treatment performed best in terms of single fruit weight, yield per plant, and fruit firmness. This study highlights the potential of nano-zinc to enhance productivity and quality in blue honeysuckle, providing a theoretical basis for selecting optimal zinc fertilizer types and particle sizes in specialty berry production, with implications for sustainable, high-quality fruit cultivation. Full article
(This article belongs to the Section Horticultural Science and Ornamental Plants)
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15 pages, 2253 KB  
Article
Sunscreen Application Mitigates Heat Stress and Enhances Fruit Quality in ‘Hass’ Avocado
by Gabriel Silva Aparecido, Valdomiro Junior Neres Santos, Felipe Rezende de Moura Ribeiro, Renata dos Santos Torelli, Bruno Henrique Leite Gonçalvez, Aloísio Costa Sampaio, Magali Leonel, Marco Antonio Tecchio, Sarita Leonel and Marcelo de Souza Silva
Horticulturae 2026, 12(5), 509; https://doi.org/10.3390/horticulturae12050509 - 22 Apr 2026
Viewed by 1204
Abstract
Brazil, as one of the world’s leading fruit producers, faces increasing challenges arising from climate change, particularly in avocado cultivation, where excessive solar radiation and high temperatures impair plant metabolism, yield, and fruit quality. This study evaluated the use of a calcium and [...] Read more.
Brazil, as one of the world’s leading fruit producers, faces increasing challenges arising from climate change, particularly in avocado cultivation, where excessive solar radiation and high temperatures impair plant metabolism, yield, and fruit quality. This study evaluated the use of a calcium and magnesium hydroxide-based sunscreen in mitigating heat stress in eight-year-old ‘Hass’ avocado trees. The experimental design was a randomized complete block design in a 4 × 8 factorial arrangement, with five replicates. Sunscreen applications were performed at full bloom and at the initial fruit development stage (18 mm). Leaf temperature, fruit drop rate, yield-related traits, fruit classification, and the percentage of fruit lesions were evaluated. Applications of the calcium and magnesium hydroxide-based sunscreen at concentrations of 3.0% and 4.5% (w/v) reduced leaf temperature and improved fruit biometric attributes compared to the control, although the maximum fruit diameter was achieved at the 2.6% concentration. The 4.5% sunscreen concentration reduced leaf temperature and fruit drop in ‘Hass’ avocado trees by 1.5 °C and 24.5%, respectively, compared with the control and decreased the percentage of small and damaged fruits. The application of sunscreen improved fruit weight and the percentage of fruits with higher market value, while the fruit diameter presented higher values at intermediate concentrations. Full article
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21 pages, 5560 KB  
Article
Spray Deposition Responses to Drone Operational Parameters in Simulated Orchard
by Lucas Barion de Oliveira, Thiago Caputti, Jessica Santos Pizzo and Andre Luiz Biscaia Ribeiro da Silva
Drones 2026, 10(4), 230; https://doi.org/10.3390/drones10040230 - 25 Mar 2026
Viewed by 1021
Abstract
Unmanned aerial vehicles (UAVs) are an alternative to traditional pesticide applications in orchards. Particularly, drones are an example of UAVs that have increased in popularity in recent years; however, relatively few studies have evaluated how spraying operation modes interact with other drone parameters [...] Read more.
Unmanned aerial vehicles (UAVs) are an alternative to traditional pesticide applications in orchards. Particularly, drones are an example of UAVs that have increased in popularity in recent years; however, relatively few studies have evaluated how spraying operation modes interact with other drone parameters within a single experimental framework. This study evaluated the effects of operation mode, application volume, flight height, and droplet size on spray coverage, droplet density, droplet spectra, and droplet size uniformity using the spraying drone DJI Agras T40 under a simulated canopy structure. A four-factorial experimental design was used; treatments included three operation modes (i.e., standard mode, fruit-tree mode, and spinning mode), two application volumes (i.e., 37.4 L/ha and 74.8 L/ha), two flight heights (i.e., 3 m and 5 m), and two droplet sizes (i.e., 150 μm and 300 μm). Operation mode was among the most influential factors affecting spray deposition quality. The spinning mode achieved the highest overall spray coverage (20.81%) and droplet density (172.44 drops/cm2), while the standard mode provided the most uniform spatial distribution. Results from the interaction analyses indicated that the parameter combination that produced the highest spray coverage within the tested ranges was an application volume of 74.8 L/ha, a flight height of 3 m, and a droplet size of 150 μm in the standard mode. For the fruit-tree mode, the highest spray coverage was observed at an application volume of 74.8 L/ha, a flight height of 5 m, and a droplet size of 300 μm. For the spinning mode, the combination associated with the highest spray coverage was 74.8 L/ha, 3 m, and 300 μm. In conclusion, the results provide data-driven guidance on how drone operational parameters influence spray deposition and can support future validation under commercial orchard conditions. Full article
(This article belongs to the Special Issue Advances of UAV in Precision Agriculture—2nd Edition)
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13 pages, 1381 KB  
Proceeding Paper
Comparative Analysis of Drying Techniques on Mineral Retention and Quality of Apricots (Prunus armeniaca L.)
by Sarvar Rejabov, Botir Usmonov, Komil Usmanov, Jaloliddin Eshbobaev, Bekzod Madaminov, Abbos Elmanov and Zafar Turakulov
Eng. Proc. 2026, 124(1), 76; https://doi.org/10.3390/engproc2026124076 - 12 Mar 2026
Cited by 1 | Viewed by 552
Abstract
This study evaluates the impact of four drying methods—open sun drying, solar drying, infrared drying, and microwave drying—on the quality attributes and elemental retention of apricots (Prunus armeniaca L.). Experimental trials were conducted in June 2024 at the Tashkent Institute of Chemical-Technology [...] Read more.
This study evaluates the impact of four drying methods—open sun drying, solar drying, infrared drying, and microwave drying—on the quality attributes and elemental retention of apricots (Prunus armeniaca L.). Experimental trials were conducted in June 2024 at the Tashkent Institute of Chemical-Technology using equal quantities of fresh apricots. Drying was continued until the moisture content, measured gravimetrically, dropped below 20% (wet basis), followed by spectroscopic analysis to determine macro- and microelement concentrations. Solar-dried apricots showed higher retention of essential nutrients in this experimental trial: potassium (2.37%), silicon (0.538%), magnesium (0.145%), calcium (0.176%), and sulfur (0.152%). In contrast, open sun drying led to significant nutrient degradation and poor visual quality. Microwave drying preserved some micronutrients but resulted in surface scorching due to uneven heating. Infrared drying yielded acceptable results but required substantial energy input. Among all methods, solar drying provided the optimal balance of high product quality and energy efficiency. The drying process required negligible electrical energy owing to exclusive reliance on solar radiation. This method supports sustainable food processing by reducing energy demand and greenhouse gas emissions while preserving nutritional quality. The results highlight solar drying as a promising, eco-friendly technique for preserving the nutritional integrity of agricultural products. These findings offer valuable scientific guidance for selecting appropriate drying technologies in the food processing industry, especially in regions with high solar potential. However, the study is limited to a single fruit variety and seasonal conditions. Full article
(This article belongs to the Proceedings of The 6th International Electronic Conference on Applied Sciences)
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18 pages, 1109 KB  
Article
Mechanical Harvest of Southern Highbush Blueberries: Influence of Harvest Interval, Delay to Impact, and Pulp Temperature at Impact on Postharvest Quality
by Adrian Berry, Steven Sargent, Merce Santana, Jeffrey Williamson and Sonya Stahl
Horticulturae 2026, 12(3), 336; https://doi.org/10.3390/horticulturae12030336 - 11 Mar 2026
Viewed by 707
Abstract
Fresh market blueberry (Vaccinium spp.) fruits are fragile and experience numerous impacts during harvest, packing, and shipping. Mechanical harvest of southern highbush blueberries (SHB) is being increasingly implemented due to rising costs and limited availability of labor. As new commercial cultivars become [...] Read more.
Fresh market blueberry (Vaccinium spp.) fruits are fragile and experience numerous impacts during harvest, packing, and shipping. Mechanical harvest of southern highbush blueberries (SHB) is being increasingly implemented due to rising costs and limited availability of labor. As new commercial cultivars become available, questions arise among growers as to their suitability for mechanical harvest. Early spring harvests in growing areas in the southeastern U.S. routinely occur when ambient temperatures exceed 30 °C. A series of experiments was conducted over a decade to determine the effects of mechanical impacts on fruit quality. These experiments employed a 60 cm drop height to induce bruising under three scenarios encountered during commercial harvest and handling. (1) Harvest interval: Nonimpacted ‘Star’ and ‘Sweetcrisp’ fruits had higher soluble solids content to titratable acidity ratios (SSC:TA) after a 7-day interval (Harvest 2) as compared with those from the initial Harvest 1. Impacted ‘Star’ blueberries from Harvest 2 were 70–100% softer during 14-d storage at 1 °C/85% relative humidity than those from Harvest 1, whereas ‘Sweetcrisp’ fruits were less affected by the harvest delay (30–40% increase in soft fruit). (2) Pulp temperature at impact: There were no differences in bruise severity for ‘Meadowlark’, ‘Colossus’, or ‘Sentinel’ due to pulp temperature at impact. Overall, impacted fruits consistently exhibited greater weight loss (3% to 9%), were softer, and had more severe bruising compared with nonimpacted controls. (3) Delays between harvest and impact: Delay-to-impact (5 or 24 h) did not affect weight loss for ‘Meadowlark’ (0.57% to 0.62%) during 4 d of storage at 5 °C. ‘Colossus’ and ‘Sentinel’, held overnight at 22 °C, lost approximately 35% to 45% more fresh weight after the 24 h delay to impact compared with those fruits with the 5 h delay to impact. Impacted blueberries exhibited significantly more severe bruising (38.5% to 84.4%) than control fruits (1.0% to 8.3%). ‘Sentinel’ was softer at harvest than the other cultivars and had the highest amount of severe bruising (82.7%), followed by ‘Meadowlark’ (52.67%) and ‘Colossus’ (42.57%). Flavor profiles varied by cultivar, with SSC:TA ratios ranging from 18 (‘Colossus’) to 21 (‘Meadowlark’) to 44 (‘Sentinel’). Immediately after impact at 15 °C, 20 °C, or 30 °C, the respiration rate (RR) for ‘Meadowlark’ increased as compared with the control fruit. RR for fruits at 5 °C or 10 °C remained fairly constant during the 8 h measurement period. These findings highlight the interactions of harvest interval, pulp temperature, and delay to impact on the postharvest quality of several commercially grown, SHB cultivars over this extended period of time. These three factors must be considered in order to develop effective strategies for mechanical harvest under the warm spring conditions encountered in the subtropical growing conditions in the southeastern U.S.A. Full article
(This article belongs to the Section Postharvest Biology, Quality, Safety, and Technology)
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20 pages, 2863 KB  
Article
Evaluation of Insecticidal Activity of Macrolide and Neonicotinoid Insecticides Against Zeugodacus tau (Walker) and Their Residue Dissipation Dynamics in Luffa cylindrica
by Xingyu Jia, Min Liu, Yaqian Shang, Hina Gul, Yuxi Wang, Yulu Mao, Shuxing Zhou, Tingzhe Sun and Jinming Zhang
Insects 2026, 17(3), 242; https://doi.org/10.3390/insects17030242 - 26 Feb 2026
Viewed by 674
Abstract
Zeugodacus tau (Walker) is a major invasive pest that targets cucurbitaceous plants and other economically significant crops, causing substantial yield and quality losses. The pest’s method of infesting the fruit, however, limits the efficacy of conventional chemical control strategies. This study comprehensively evaluates [...] Read more.
Zeugodacus tau (Walker) is a major invasive pest that targets cucurbitaceous plants and other economically significant crops, causing substantial yield and quality losses. The pest’s method of infesting the fruit, however, limits the efficacy of conventional chemical control strategies. This study comprehensively evaluates the insecticidal efficacy of seven insecticides from two key classes—macrolides and neonicotinoids—against various developmental stages of Z. tau. Additionally, it investigates the distribution and degradation patterns of pesticide residues on sponge gourd. Laboratory toxicity tests showed notable differences in sensitivity among the three pest stages. Larvae were the most sensitive to insecticides, followed by adults, with eggs being relatively resistant. Four macrolide insecticides showed high activity against both adults and larvae, while three neonicotinoids displayed low activity against eggs, larvae, and adults of Z. tau. Field residue trials using common recommended dosages demonstrated rapid degradation of pesticide residues on sponge gourd, with residue levels dropping below the national maximum residue limits (MRLs) within 2 to 24 h after application. Further results indicated that pesticide residues accumulated mainly on the peel, with low or undetectable levels in the flesh. Among the macrolide insecticides, residues were nearly undetectable, whereas neonicotinoids, due to their systemic nature, sometimes showed trace residues in the fruit flesh. Oviposition hole measurements revealed an average depth of 0.57 ± 0.29 cm, just beneath the skin, with the hole’s structure effectively protecting the eggs laid by females. Based on the comprehensive analysis of insecticide toxicity and residue degradation, control strategies should primarily target adults. Macrolide insecticides, which have low residue risks and high efficacy against both adults and larvae, are recommended as the preferred option for management. This study, considering the unique damage characteristics of Z. tau, offers valuable insights into chemical control strategies, focusing on insecticide sensitivity at different life stages, residue distribution, and degradation, providing important references for the scientific use of pesticides and the optimization of pest management approaches. Full article
(This article belongs to the Special Issue The 3M Approach to Insecticide Resistance in Insects)
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22 pages, 9889 KB  
Article
Hyperspectral Estimation of Apple Canopy SPAD Values Based on Optimized Spectral Indices and CEO-LSSVM
by Kaiyao Hou, Ziyan Shi, Wei Lou, Bo Xiao and Xu Li
Agronomy 2026, 16(4), 490; https://doi.org/10.3390/agronomy16040490 - 23 Feb 2026
Viewed by 604
Abstract
Leaf chlorophyll content (LCC) is a key physiological parameter affecting plant growth and development. Rapid and non-destructive monitoring of LCC using hyperspectral remote sensing is crucial for promoting precision agriculture. In this study, hyperspectral data of apple canopy leaves at different phenological stages [...] Read more.
Leaf chlorophyll content (LCC) is a key physiological parameter affecting plant growth and development. Rapid and non-destructive monitoring of LCC using hyperspectral remote sensing is crucial for promoting precision agriculture. In this study, hyperspectral data of apple canopy leaves at different phenological stages were collected alongside their corresponding SPAD values (representing LCC) to construct a dataset. Two types of spectral features were extracted: (1) optimized spectral index combinations; and (2) feature bands selected using the Successive Projections Algorithm (SPA). Based on these features, three machine learning models—Support Vector Machine (SVM), Least Squares Support Vector Machine (LSSVM), and Chaos Evolution Optimization-enhanced LSSVM (CEO-LSSVM)—were developed to estimate SPAD values. The results indicate that the constructed optimal spectral index combinations exhibit superior sensitivity in SPAD estimation compared to the feature bands selected by SPA. Specifically, during the physiological fruit drop stage, the CEO-LSSVM model based on spectral indices achieved a test set R2 of 0.851, surpassing the SPA-based model (R2 = 0.813). Regarding model performance, the CEO-LSSVM demonstrated the highest accuracy and robustness across all stages. In the fruit drop period, using optimized spectral indices, it achieved an RMSE of 1.338, significantly outperforming the LSSVM (RMSE = 1.703) and SVM (RMSE = 2.409) models. This superiority was further evident in the fruit enlargement stage, where the CEO-LSSVM model reached a peak test set R2 of 0.868 and the lowest RMSE of 1.254. The integrated model combining optimized spectral indices and CEO-LSSVM provides an efficient and high-precision approach for hyperspectral SPAD estimation in apple canopies, effectively addressing the challenges of inversion modeling in arid oasis environments. Full article
(This article belongs to the Section Precision and Digital Agriculture)
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19 pages, 2091 KB  
Article
Evaluation of the Antifungal Potential of Different Photorhabdus Species Against Monilinia laxa and Colletotrichum fioriniae
by Emre Şen, Tímea Tóth, Szabolcs Ádám and Tamás Lakatos
J. Fungi 2026, 12(2), 159; https://doi.org/10.3390/jof12020159 - 22 Feb 2026
Viewed by 832
Abstract
Monilinia laxa and Colletotrichum fioriniae are major fungal pathogens causing brown rot and anthracnose in stone fruits and shell fruits, leading to significant economic losses. Chemical fungicides are widely applied but can result in resistance development, environmental contamination, and food safety concerns. Biological [...] Read more.
Monilinia laxa and Colletotrichum fioriniae are major fungal pathogens causing brown rot and anthracnose in stone fruits and shell fruits, leading to significant economic losses. Chemical fungicides are widely applied but can result in resistance development, environmental contamination, and food safety concerns. Biological control using entomopathogenic bacteria (EPB) of the genus Photorhabdus has emerged as an eco-friendly alternative. This study evaluated the in vitro antifungal activity of selected Photorhabdus species (P. kayaii 1723B, P. temperata 3017, P. cinerea 3086, P. laumondii 3196, and P. thracensis 3210) against M. laxa (M3) and C. fioriniae (VV081) using drop-to-drop confrontation and poisoned agar assays. Effects of fermentation time, preparation mode (original vs. centrifuged and filtered), and concentration (5, 10, 20%) were examined. Species-specific inhibition was observed, with Median Inhibition Index values indicated relatively higher antifungal activity for P. thracensis 3210 against M. laxa (0.718) and C. fioriniae (0.552), followed by P. cinerea 3086 (0.643 and 0.552) and P. kayaii 1723B (0.629 and 0.541). Fermentation time and preparation mode influenced antifungal activity in a strain-dependent manner, with longer fermentation periods and original culture preparations generally showing stronger inhibitory trends. Higher concentrations, especially 20%, were often associated with increased inhibition, although the magnitude of these effects varied among strain–pathogen combinations. Overall, these findings demonstrate that the strain- and pathogen-specific nature of antifungal responses in Photorhabdus, supporting their potential as components of targeted biological control strategies rather than uniform broad-spectrum agents. Full article
(This article belongs to the Section Fungal Pathogenesis and Disease Control)
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19 pages, 6613 KB  
Article
Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum (Prunus domestica) Brown Spot in Xinjiang, China
by Shuaishuai Sha, Qiuyan Han, Hongyue Li, Wenwen Gao, Jiyuan Ma, Lingkai Xu, Canpeng Fu and Pan Xie
J. Fungi 2026, 12(1), 69; https://doi.org/10.3390/jof12010069 - 15 Jan 2026
Cited by 1 | Viewed by 979
Abstract
European plum (Prunus domestica) orchards in the Kashi region, Xinjiang, China, suffer from fruit brown spot disease. The disease typically appears as red spots on the fruit surface that expand into brown necrotic lesions; affected fruit flesh can shrink, and fruits [...] Read more.
European plum (Prunus domestica) orchards in the Kashi region, Xinjiang, China, suffer from fruit brown spot disease. The disease typically appears as red spots on the fruit surface that expand into brown necrotic lesions; affected fruit flesh can shrink, and fruits can harden and drop. We isolate and identify pathogens associated with this disease in this plum from five Kashi counties. Of 210 fungal isolates obtained through standard tissue isolation, Alternaria accounted for 84.8%, with the remainder comprising species of Aspergillus (9.5%), Diplodia (3.3%), and Neoscytalidium (2.4%). Using PCR amplification and sequencing of five loci, pathogens were identified using multi-gene phylogenetic analyses, combined with observations of colony and spore morphology. Multi-locus sequences of Alternaria isolates were highly homologous to those of the Alternaria alternata type strain, and we refer them to an A. alternata species complex. Pathogenicity tests confirm that Alternaria isolates reproduce brown spot symptoms on European plum fruits. By demonstrating that Alternaria is the primary pathogen causing brown spot disease in European plum in Xinjiang, we clarify both the fungal species composition and taxonomic placement of the dominant pathogen associated with this disease. Full article
(This article belongs to the Section Fungal Genomics, Genetics and Molecular Biology)
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10 pages, 1644 KB  
Proceeding Paper
Heat Stress in Chillies: Integrating Physiological Responses and Heterosis Breeding Approaches for Enhanced Resilience
by Inaba Hawraa, Muhammad Azam Khan, Muhammad Tahir Akram, Rashid Mehmood Rana, Feroz Ahmed Tipu, Israr Ali, Hina Nawaz and Muhammad Hashir Khan
Biol. Life Sci. Forum 2025, 51(1), 12; https://doi.org/10.3390/blsf2025051012 - 6 Jan 2026
Cited by 1 | Viewed by 1115
Abstract
Chilli (Capsicum annuum) is a popular spice and vegetable crop of significant economic importance that is cultivated worldwide in warm and humid climatic zones. Although chilli is a thermophilic crop, its quality and yield potential are significantly affected due to various [...] Read more.
Chilli (Capsicum annuum) is a popular spice and vegetable crop of significant economic importance that is cultivated worldwide in warm and humid climatic zones. Although chilli is a thermophilic crop, its quality and yield potential are significantly affected due to various abiotic factors, including extremely fluctuating temperatures beyond the optimum temperatures (18–30 °C). Global warming and anthropogenic activities lead to adverse climatic changes, imposing severe stress on growth, development, and productivity. High temperatures above 43–45 °C adversely affect chilli crops, especially during the reproductive stages, by causing immature fruit dropping, poor seed vigour, reduced number of flowers, flower abscission, aborted reproductive organs, reduced fruit set, and significant yield loss by 50%. Therefore, to reduce quantitative and qualitative losses, heat management is necessary from April to June in Pakistan, when the temperature rises beyond 40 °C. For heat management, the hybridisation of heat-resilient and high-yielding genotypes to develop heat-tolerant high-yielding hybrids appears to be a rational approach. These genetically improved hybrids inherit such characteristics that assist in maintaining vigorous growth, fruit quality, and stable yield without significant yield losses even under heat-stressed conditions. Hence, the thermotolerant chilli hybrids developed through hybridisation help to satisfy the escalating demand for chilli and guarantee the financial stability of farmers. Full article
(This article belongs to the Proceedings of The 9th International Horticulture Conference & Expo)
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30 pages, 8582 KB  
Article
Machine Learning Approaches for Assessing Avocado Alternate Bearing Using Sentinel-2 and Climate Variables—A Case Study in Limpopo, South Africa
by Muhammad Moshiur Rahman, Andrew Robson and Theo Bekker
Remote Sens. 2025, 17(24), 3935; https://doi.org/10.3390/rs17243935 - 5 Dec 2025
Viewed by 1584
Abstract
Alternate (irregular) bearing, characterized by large fluctuations in fruit yield between consecutive years, remains a major constraint to sustainable avocado (Persea americana) production. This study aimed to assess the potential of satellite remote sensing and climatic variables to characterize and predict [...] Read more.
Alternate (irregular) bearing, characterized by large fluctuations in fruit yield between consecutive years, remains a major constraint to sustainable avocado (Persea americana) production. This study aimed to assess the potential of satellite remote sensing and climatic variables to characterize and predict alternate bearing patterns in commercial orchards in Tzaneen, Limpopo Province, South Africa. Historical yield data (2018–2024) from 46 “Hass” avocado blocks were analyzed alongside Sentinel-2 derived vegetation indices (NDVI, GNDVI, NDRE, CIG, CIRE, EVI2, LSWI) and flowering indices (WYI, NDYI, MTYI). To align temporal scales, all VIs and FIs were aggregated into eight quarterly averages from the two years preceding each yield year and spatially averaged across each orchard block. Climatic predictors including maximum temperature (Tmax), minimum temperature (Tmin), vapor pressure deficit (VPD), and precipitation were screened against historical yields to identify critical periods, with June–October emerging as the most influential months, and these variables were aggregated accordingly to match annual alternate bearing patterns. Five machine learning (ML) algorithms—Random Forest, XGBoost, CATBoost, LightGBM, and TabPFN—were trained and tested using a Leave-One-Year-Out (LOYO) approach. Results showed that VPD, Tmin, and Tmax during the flowering period (July–September) were the most influential variables affecting subsequent yields. TabPFN achieved the highest predictive accuracy (Accuracy = 0.88; AUC = 0.95) and strongest temporal generalization. Spectral gradients between flowering and early fruit drop were lower during “on” years, reflecting stable canopy vigor. This combined use of remote sensing and climatic variables in a ML framework represents a novel approach, and the findings demonstrate that integrating remote sensing and climatic indicators enables early discrimination of “on” and “off” years, supporting proactive orchard management and improved yield stability. Full article
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14 pages, 3098 KB  
Article
Interaction of BPC1 and ALDH2 Affects Natural De-Astringency in Chinese PCNA Persimmon (Diospyros kaki)
by Junchi Xu, Xia Li, Li Zhang, Fei Zhang and Shiping Wang
Horticulturae 2025, 11(12), 1435; https://doi.org/10.3390/horticulturae11121435 - 27 Nov 2025
Viewed by 696
Abstract
Pollination-constant non-astringent (PCNA) persimmons have significant commercial value due to their natural de-astringency trait. The Chinese PCNA (C-PCNA) type is particularly valuable for genetic improvement because this trait is controlled by dominant genes. However, the regulatory mechanism underlying this trait remains unclear. Our [...] Read more.
Pollination-constant non-astringent (PCNA) persimmons have significant commercial value due to their natural de-astringency trait. The Chinese PCNA (C-PCNA) type is particularly valuable for genetic improvement because this trait is controlled by dominant genes. However, the regulatory mechanism underlying this trait remains unclear. Our previous research identified ALDH2 (acetaldehyde dehydrogenase 2, a key gene downstream of acetaldehyde metabolism) as being negatively correlated with natural de-astringency in C-PCNA persimmon, and revealed its interaction with DkBPC1, a transcription factor from the BBR/BPC (BARLEY B RECOMBINANT/BASIC PENTA CYSTEINE, abbreviated as the BPC protein family) family whose function had not been experimentally validated. The full-length cDNA of DkBPC1 was isolated from ‘Luotian Tianshi’ (C-PCNA type). A dual-luciferase assay demonstrated that DkBPC1 significantly enhances the promoter activity of DkALDH2b. Subcellular localization conducted in tobacco confirmed that DkBPC1 is localized in the nucleus. Transient overexpression of DkBPC1 in C-PCNA leaves and fruit discs resulted in a significant increase in soluble tannin content, a significant decrease in insoluble tannin content, and a notable upregulation of DkALDH2b gene expression. Conversely, transient knockdown of DkBPC1 expression in C-PCNA leaves led to a dramatic drop in soluble tannin content and a significant increase in insoluble tannin content. These results indicate that BPC1 can increase the conversion of soluble tannins to insoluble tannins by downregulating the expression of the DkALDH2 gene, thereby promoting natural de-astringency in C-PCNA persimmon. Full article
(This article belongs to the Special Issue Genetic Breeding and Diversity of Fruit Germplasm Resources)
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26 pages, 1595 KB  
Article
Early Vegetative Response and Fruit Quality Modulation by Fruit Thinning and Weed-Control Mesh in Citrus sinensis CV. ‘Navelina’
by Carlos Giménez-Valero, Dámaris Núñez-Gómez, Pilar Legua, Juan José Martínez-Nicolás, Vicente Lidón Noguera and Pablo Melgarejo
Horticulturae 2025, 11(11), 1387; https://doi.org/10.3390/horticulturae11111387 - 18 Nov 2025
Viewed by 1008
Abstract
Cultivation practices such as fruit thinning and soil management with ground covers are commonly applied in Citrus orchards, yet their physiological impact on young trees remains poorly documented. This study evaluated the effects of manual fruit thinning and weed-control mesh on vegetative growth, [...] Read more.
Cultivation practices such as fruit thinning and soil management with ground covers are commonly applied in Citrus orchards, yet their physiological impact on young trees remains poorly documented. This study evaluated the effects of manual fruit thinning and weed-control mesh on vegetative growth, fruit development, and leaf mineral composition of Citrus sinensis L. Osbeck cv. ‘Navelina’ grafted on Citrus macrophylla. A six-month field experiment was conducted in southeastern Spain under semi-arid Mediterranean conditions using six treatments that combined different soil coverage and subsurface drainage systems. After physiological fruit drop, trees were standardized to ten fruits per plant. Vegetative parameters (canopy and trunk dimensions), fruit growth (size, juice content), and foliar nutrient concentrations were monitored. Trees with ground cover showed significantly greater canopy expansion and juice yield compared to uncovered controls. A negative correlation between fruit number and canopy-to-fruit volume ratio highlighted the trade-off between vegetative vigor and fruit load. Foliar analysis revealed lower micronutrient concentrations (Fe, Mn, B, Zn) in uncovered trees, suggesting reduced nutritional status. These findings demonstrate that combining early thinning with weed-control mesh promotes vegetative vigor, improves juice yield, and enhances nutrient uptake, providing practical insights for optimizing orchard establishment and early Citrus productivity in water-limited environments. Full article
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15 pages, 2008 KB  
Article
Calcium Reduces Fruit Abscission in Persimmon by Targeting Cell Wall Integrity
by Andrés Marzal, Julia Morales, Amparo Primo-Capella, Almudena Bermejo, Amparo Martínez-Fuentes and Ana Quiñones
Plants 2025, 14(22), 3482; https://doi.org/10.3390/plants14223482 - 14 Nov 2025
Cited by 1 | Viewed by 1641
Abstract
In the Mediterranean region, the persimmon cultivar ‘Rojo Brillante’ may experience up to four waves of fruit drop. The first is a physiological event during fruit set that is common in woody species, while the subsequent waves are induced by rising temperatures and [...] Read more.
In the Mediterranean region, the persimmon cultivar ‘Rojo Brillante’ may experience up to four waves of fruit drop. The first is a physiological event during fruit set that is common in woody species, while the subsequent waves are induced by rising temperatures and prolonged summer water stress. These summer drops represent the main limiting factor, leading to yield losses of up to 90%. Organ abscission is a complex process regulated by genetic, hormonal, nutritional, and environmental factors. We hypothesise that calcium (Ca) plays a protective role in the abscission zone (AZ) by inhibiting cell wall-degrading enzymes such as polygalacturonase (PG) and pectin methylesterases (PMEs). Calcium applications every 15 days from anthesis onwards significantly reduced fruit drop. Treatments preserved polar auxin transport—through DkPIN1 expression—and inhibited stage C of the abscission process, decreasing the relative expression of the DkIDL6 gene in the AZ. Moreover, PME and PG activities were significantly lower in Ca-treated fruits, confirming the stabilising effect of calcium on AZ integrity. In summary, pre-anthesis calcium sprays reduced premature fruit drop by about 30% under heat–drought stress by down-regulating key abscission genes (DkIDL6, DkPG20, DkPME41) and preserving cell wall integrity and fruit firmness, supporting the use of Ca treatments as a climate-smart approach to stabilise persimmon yield. Full article
(This article belongs to the Section Crop Physiology and Crop Production)
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Article
Financial Mechanisms and Risk-Based Modeling of Energy Efficiency and Renewable Energy Adoption in Households
by Olha Prokopenko, Galyna Trypolska, Iryna Bashynska, Oleksandr Telizhenko, Włodzimierz Strelcow, Yevhen Kovalenko, Svitlana Lytvynenko and Anna Woźna
Energies 2025, 18(21), 5799; https://doi.org/10.3390/en18215799 - 3 Nov 2025
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Abstract
The research aims to evaluate financial instruments on household uptake of energy efficiency and renewable energy towards different risk scenarios. The study addresses the problem of behavioral response to financial incentives when technological, financial, or institutional risks are perceived as continuous. Two sophisticated [...] Read more.
The research aims to evaluate financial instruments on household uptake of energy efficiency and renewable energy towards different risk scenarios. The study addresses the problem of behavioral response to financial incentives when technological, financial, or institutional risks are perceived as continuous. Two sophisticated models were used for the analysis to quantify the effect of subsidies, green loans, personal income, energy costs, and governmental support for energy efficiency and renewable energy uptake. The research data came from the UK, Estonia, Germany, Poland, and Ukraine between 2022 and 2024. The results suggest that countries experiencing drops in risk indices with strong institutional support, such as Germany and the UK, had maximum improvement in energy efficiency (as high as 598.72 kWh saved a year) and renewable energy implementation rates (above 30%). Countries posing high risk, like Ukraine, require more potent and custom-made strategies to achieve comparable advances compared to a less-risky environment. The evidence indicates that even financial mechanisms are most fruitful if they are complemented by risk management tactics. With these results, policymakers can proceed with useful information in formulating economically appropriate strategies that rely on realistic assumptions of behavior. Full article
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