Search Results (10,940)

This study presents a systematic bibliometric analysis and critical review of assessment indicators and multi-criteria decision-making methods for rural energy systems from 2010 to 2025. It examines the evolving definitions and regional variations in these indicators and methods. The research hotspots of rural energy systems have shifted from basic rural electrification to multi-dimensional assessment indicators and hybrid multi-criteria decision-making methods. The assessment indicators for rural energy systems demonstrate a marked imbalance, dominated by economic and technical dimensions. Specifically, economic evaluations for rural energy systems frequently utilize net present cost and levelized energy cost, shifting from static capital comparisons to comprehensive lifecycle assessments. Meanwhile, loss of power supply probability is identified as the primary inherent constraint among technical assessment indicators for rural energy systems. Geographically, assessment indicators for rural energy systems priorities exhibit significant divergence. Developing regions prioritize basic power supply and affordability, whereas developed regions focus on grid stability and market risk resilience. In addition, environmental evaluations for rural energy systems remain fixated on carbon emissions. Developed nations emphasize global climate benefits, while developing nations focus on localized dividends like indoor air quality improvement. Critically, despite an increasing focus on rural livelihoods, social indicators remain systematically marginalized in rural energy systems, leading to the neglect of local requirements and increasing technical risks. The field of rural energy system assessment is advancing toward multi-criteria decision-making indicators. Future methodologies must integrate robust, dynamic adaptive mechanisms that respond to evolving developmental priorities in order to effectively address inherent data scarcity and complex socio-economic uncertainties of rural energy systems. Full article

Hepatitis B virus (HBV) is the smallest partially double-stranded, reverse-transcribing DNA virus, with four open reading frames (ORFs) encoding viral proteins. It is classified into nine geographically distributed genotypes (A–I). In Kenya, the molecular characterization of HBV among patients seeking medical care remains poorly defined. This observational study aimed to characterize HBV among patients seeking medical care in Kenya’s endemic region, focusing on circulating genotypes and ORF mutations. Serum samples were collected from the outpatient departments of selected health facilities, with demographic and clinical information extracted from patients’ medical records. Hepatitis B surface antigen (HBsAg) was tested at the facilities, and 85 HBsAg-positive samples were collected for molecular analysis. The basal core promoter and pre-core (BCP/PC), polymerase, and surface regions of the viral genome were amplified and sequenced to determine genotypes and to profile their mutations. Out of 85 HBsAg-positive samples, 38 samples tested positive for HBV DNA, and 26 samples were successfully sequenced. HBV genotype A was prevalent at 73.1% (19/26), followed by genotype D at 23.1% (6/26), and genotype E at 3.8% (1/26). Genotype A sequences clustered with both A1 Asian and African subgenotypes, whereas genotype D clustered with subgenotypes D6 and D1. All HBV genotype A, D, and E sequences were serotypes adw2, ayw2, and ayw4, respectively. HBV core promoter mutations (A1762T/G1764A) were detected in both genotype D and genotype A isolates. The pre-core G1896A mutation was highly prevalent in genotype D samples (5/6; 83.3%) but was not observed in genotypes A or E. Analysis of mutations within the “a” determinant region revealed genotype-specific patterns: genotype A predominantly harbored V14A, P46H, S58C, and P67Q substitutions; genotype E showed N59S; and genotype D exhibited V14A, C69stop, S104T, and W182stop mutations. Two drug resistance mutations (V191I and A194T) were present in two chronic patients, one with genotype A and the other with genotype D. In conclusion, HBV genotypes A and D are the most prevalent among Kenyan patients with chronic HBV infection. The presence of point mutations in the ORFs among patients seeking medical care highlights the need for molecular surveillance to better understand the viral diversity and its potential clinical and public health implications. Full article

Environmental noise is increasingly recognized as a major environmental and public health challenge, with road traffic identified as the dominant source of acoustic pollution across Europe. In this context, noise mitigation is directly linked to sustainable development goals related to human health and urban sustainability. Noise barriers are among the most widely implemented mitigation strategies; however, their spatial distribution and adequacy remain poorly documented, limiting their effectiveness for sustainable territorial planning. This study develops the first georeferenced database of highway noise barriers in Andalusia (Spain) and applies a reproducible, transdisciplinary geospatial workflow integrating field surveys, remote-sensing tools, and Geographic Information Systems (GIS). A total of 110 barriers were mapped, classified by material, geometry, and surrounding land use, and analyzed in relation to sensitive receptors, including dwellings, schools, and hospitals. Results show that only 1.6% of the Andalusian highway network is currently protected by noise barriers, with strong territorial disparities: over 50% of all structures are concentrated along coastal metropolitan corridors, while extensive inland areas remain unprotected. Misalignments were also detected between barrier placement and officially reported high-exposure segments, indicating limited correspondence between infrastructural deployment and planning-designated priority areas. Beyond generating a comprehensive regional dataset, the proposed methodology provides a scalable basis for national and European initiatives seeking to harmonize the mapping and assessment of noise-mitigation infrastructures. By offering an open-access, transferable framework, this work contributes to a more equitable distribution of environmental protection measures and supports policy professionals, environmental managers, and planners in advancing healthier and more sustainable urban and transport systems. Full article

Background/Objectives: Measles is a highly contagious infectious disease. Maintaining a high level of measles immunity in the population is essential due to the extremely high transmissibility of the measles virus (MV). We analyzed the MV seroprevalence in the Croatian population. Methods: A total of 1998 individuals tested consecutively for MV antibodies from 2015 to 2025 inclusive were included. MV IgG antibodies were detected using a commercial ELISA. Results: The overall seroprevalence rate was 75.1%, with significant yearly differences, ranging from 50.0 to 86.5%, and a declining trend since 2023. No differences were observed between sexes (males 71.6%, females 71.9%). The seroprevalence increased with age, from 60.9% in the 1–10 age group to 91.1% in the 61+ age group. Significant geographic differences were found, with higher seropositivity rates in coastal regions compared to continental regions (81.3 and 71.0%, respectively). Settlement type (urban, suburban/rural) was not associated with the MV seroprevalence. The results of the multivariable logistic regression analysis showed that year, age, and region were significantly associated with IgG seropositivity. Each additional calendar year was associated with lower odds of IgG positivity (OR = 0.82), while each additional year of age was associated with higher odds (OR = 1.04). Region was also a significant predictor (OR = 1.65), while settlement was not significantly associated with seropositivity after adjustment for other variables. Conclusions: Croatia has historically maintained high measles coverage, but lower uptake in some regions and age groups may be creating immunity gaps. The lower post-2022 seropositivity underscores significant immunity gaps, particularly among highly susceptible groups that were exposed to outbreaks and tested during that period. Full article

This study explored the application of carbon stable isotopes for tracing the geographical origin of Monochamus alternatus, an insect vector responsible for spreading pine wilt disease. The primary vector of pine wilt disease, an aggressive disease caused by the pine wood nematode and affecting pine forests, is Monochamus alternatus. Samples of Monochamus alternatus were collected from 12 provinces across China, and their carbon isotope ratios (δ¹³C) were measured. By analyzing the correlation between these ratios and various environmental factors, including latitude, longitude, altitude, and bioclimatic conditions, it was found that precipitation seasonality and solar radiation were the most important factors influencing the carbon isotope ratio of Monochamus alternatus. The spatial distribution of Monochamus alternatus carbon isotopes in China was predicted using the co-Kriging interpolation method, incorporating these two environmental variables. The findings revealed a gradient in the carbon isotope ratio of Monochamus alternatus, which could help differentiate the species across various geographical regions in China. Full article

Background: Older adults (aged 65 and older) faced a disproportionate burden of mortality during the COVID-19 pandemic, yet substantial geographical and sociodemographic disparities in vaccine uptake persisted within this vulnerable population. Objective: To examine the temporal dynamics of COVID-19 vaccination rates among older adults and investigate the association between vaccination uptake and neighborhood-level social determinants of health (SDOHs), including disability and poverty. Methods: COVID-19 vaccination data for older adult residents in Jefferson County, Kentucky, were obtained from the Kentucky Immunization Registry (KYIR). ZIP-code-level vaccination rates were calculated at three time points: 28 February 2021 (Q1), 31 May 2021 (Q2), and 31 May 2022 (Q6). The rates were linked to 2021 American Community Survey (ACS) ZIP code-level estimates of disability, poverty, and household composition. Two-dose COVID-19 vaccination rates stratified by race, ethnicity, and geographic region were used as outcome measures. Pearson correlation coefficients, bivariate, and multivariate linear models were used to estimate the association between COVID-19 vaccination rates and the SDOHs at the ZIP code level. Results: Among the estimated 139,222 older adults, overall two-dose vaccination rates rose from 22.4% in Q1 to 77.5% by Q6. Significant regional disparities were observed early in the campaign, with Q1 rates ranging from 12.6% in the Southwest to 35.4% in the Inner East county regions. Bivariate analyses showed ZIP-code-level disability and poverty rates were negatively associated with ZIP-code-level vaccination uptake in Q1 (disability slope: −0.38; 95% CI, −0.63 to −0.13; poverty slope: −0.36; 95% CI, −0.65 to −0.07). By Q6, the negative association between disability and vaccination had weakened significantly and was no longer statistically significant, while the negative association between poverty rate and vaccination rate remained persistent across all time points. Conclusions: The disability-associated gaps in older adults’ vaccination rates were dynamic and narrowed over time, whereas the poverty-associated gaps remained persistent and static. The low uptake observed among Black and Hispanic older adults in historically underserved areas suggests that understanding the specific factors that most negatively associate with vaccination rates in these populations, such as specific disabilities, may mitigate structural barriers. Future public health interventions should prioritize socioeconomically disadvantaged neighborhoods and account for the evolving association of functional impairments and healthcare access. Full article

Leeches (Hirudinea) are ecologically important annelids that interact with a wide range of aquatic vertebrates, yet their diversity, distribution, and epidemiological relevance remain poorly documented in North Africa. Here, we provide a comprehensive synthesis of freshwater and marine leech species reported from the Maghreb (Algeria, Tunisia, and Morocco), based on an extensive review of the available literature. In total, 21 species belonging to 13 genera and four families (Glossiphoniidae, Erpobdellidae, Hirudinidae, and Piscicolidae) are documented, with updated information on their ecology, host associations, and geographic distribution. In addition to this regional checklist, we report the first confirmed case of Batracobdella algira heavy parasitism on the Berber toad (Sclerophrys mauritanica) in Algeria. A single adult toad was found heavily infested by multiple leeches (n = 17), some of which bore spermatophores attached near the reproductive opercula, suggesting possible in situ mating behavior on the host. The high infestation observed in this single specimen may constitute an outlier, requiring further sampling to assess the effect of leeches on the anuran population in the region. By integrating faunistic data with a novel field observation, this study highlights the overlooked leech biodiversity in the Maghreb and suggests their possible ecological and epidemiological significance. Our findings emphasize the need for further investigations into leech–host interactions, pathogen carriage, and their implications for amphibian conservation and One Health in North Africa. Full article

Background/Objectives: Eleocharis ussuriensis Zinserl. is a perennial riparian sedge widely distributed in Northeast Asia and a dominant component of freshwater vegetation in South Korea. However, the intraspecific genetic structure of this species across contrasting hydrological habitats remains insufficiently understood. This study aimed to develop novel SSR markers from whole-genome data and investigate genetic variation and population structure among E. ussuriensis populations in South Korea. Methods: Twenty-one novel simple sequence repeat (SSR) markers were developed from whole-genome sequence data and applied to analyze genetic variation in 120 individuals from 6 populations. Genetic diversity, differentiation, and gene flow were estimated using allele-frequency-based metrics, and population genetic structure was further evaluated using spatial information derived from geographic coordinates. Results: A total of 201 alleles were detected, with a mean polymorphism information content (PIC) of 0.759, indicating high marker informativeness. Mean genetic diversity across populations showed observed heterozygosity (Ho = 0.360) and expected heterozygosity (He = 0.281), while multilocus genotype ratios (G/N) ranged from 0.30 to 1.00 among populations. Genetic differentiation was substantial (F_ST = 0.373–0.669; Jost’s D = 0.540–0.997). Mantel tests revealed that genetic differentiation was significantly correlated with geographic distance (r = 0.67, p < 0.001). Both allele-frequency-based and spatially explicit approaches suggested genetic structuring among populations. Conclusions: The results suggest spatial tendencies in genetic structure among populations, reflecting patterns of allele distribution across regions. These findings provide baseline information on genetic variation in E. ussuriensis and may contribute to a better understanding of its ecological dynamics. Full article

Neospora caninum is an apicomplexan parasite with a broad geographical distribution and a complex life cycle. It can cause infectious abortions in a variety of animals in the major livestock-producing nations, resulting in huge economic losses to the livestock industry. Shanxi Province in north China is one of China’s important livestock-producing provinces, but the data on the prevalence of N. caninum in sheep and goats in this province was not available prior to the present investigation. To fulfill this gap in our knowledge, serum samples were collected from 504 sheep and 300 goats across 11 cities representing three distinct geographical regions of Shanxi Province. A commercially available indirect enzyme-linked immunosorbent assay (iELISA) kit was used to determine the N. caninum prevalence by detecting N. caninum-specific IgG antibodies. Results showed that a total of 24 sheep (4.8%; 95% CI: 2.9–6.6) and 8 goat samples (2.7%; 95% CI: 0.8–4.5) tested positive for N. caninum antibodies. Geographical distribution was identified as the predominant risk factor influencing N. caninum infection in sheep and goats in Shanxi Province, with seroprevalence of N. caninum ranging from 0% to 16.7% across different sampling sites. This study reports the seroprevalence of N. caninum infection in sheep and goats in Shanxi Province for the first time, providing baseline data for the prevention and control of N. caninum infection in this northern province of China. Full article

Hippophae tibetana Schltdl. is a cold-tolerant deciduous shrub endemic to the Tibetan Plateau, playing a vital ecological role in high-altitude environments. This study utilized the Biomod2 platform to model its current and future potential distribution under climate change, integrating 34 environmental variables across bioclimatic, topographic, edaphic, anthropogenic, and ultraviolet (UV) dimensions. Among ten candidate species distribution models (SDMs), the random forest (RF) algorithm exhibited the highest predictive accuracy and stability. An ensemble model (EM) combining RF, GBM, MARS, and FDA further improved predictive performance (ROC = 0.992, TSS = 0.923, and Kappa = 0.886). Key determinants of habitat suitability included altitude, temperature, UV radiation, and biodiversity, with RF response curves revealing distinct nonlinear thresholds. Optimal suitability occurred at around a 4000 m elevation, decreasing beyond this range, while temperature and UV exhibited similar unimodal responses. Under the SSP2-4.5 climate scenario, the suitable habitat is projected to expand from the 2050s to the 2090s, particularly in eastern Qinghai, southwestern Gansu, northwestern Sichuan, and central–southern Tibet. The species’ distribution centroid is anticipated to shift southwestward toward Qinghai Province, with more rapid migration projected after the 2050s. These findings underscore the complex interplay of environmental factors shaping H. tibetana distribution and offer valuable insights for conservation planning in the ecologically fragile Tibetan Plateau. Full article

The global demand for Salvia miltiorrhiza Bunge in the botanical medicine market is steadily increasing. However, its production has long relied on asexual root propagation, making it highly susceptible to germplasm degradation. Transitioning to seed reproduction offers the advantage of genetic renewal, yet it is constrained by unstable seed vigor and slow seedling growth. In the present study, comprehensive physiological and microbiome analyses of S. miltiorrhiza seeds from 14 regions across 7 provinces in China were conducted to elucidate the association between the seed microbiome and vigor, and to identify plant growth-promoting (PGP) strains. The results demonstrated: (1) Seed physical traits and germination characteristics varied significantly across geographic origins. Seed vigor, exhibiting the highest coefficient of variation, served as a key parameter reflecting germination quality. (2) High-vigor seeds harbored distinct microbial communities characterized by higher diversity indices, greater network complexity, and the significant enrichment of potentially beneficial bacteria (e.g., Pseudomonas). (3) Through correlation-directed screening of isolated pure cultures, Pseudomonas mendocina P-6 and Enterobacter ludwigii BM-12 were identified as exhibiting robust, multi-trait PGP capacity. In planta validation showed that these two strains significantly promoted the growth of 1-month-old S. miltiorrhiza seedlings, increasing total fresh weight by 33.9–71.3%. This study reveals the microecological drivers of seed vigor and provides candidate strains for inoculant development, thereby supporting the sustainable, seed-based propagation of S. miltiorrhiza. Full article

Background/Objectives: Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli are priority antimicrobial-resistant pathogens with significant implications for food safety and public health. Food-producing animals and their derived products represent a key interface for zoonotic transmission, yet prevalence data across Nigeria remain fragmented and unsynthesized. This systematic review and meta-analysis assessed the prevalence, species distribution, geographical patterns, and detection methods of ESBL-producing E. coli in food-producing animals and animal-derived food products across Nigeria. Methods: A comprehensive search of PubMed, Web of Science, Scopus, and African journals online was conducted for studies published between January 2000 and January 2026, following PRISMA 2020 guidelines. Twenty eligible studies collectively analyzed 5104 samples, and 984 ESBL-positive isolates were included in the meta-analysis. Results: The overall pooled prevalence of ESBL-producing E. coli was 17.0% (95% CI: 13.0–21.0%; I² = 89.4%). Subgroup analysis by animal species revealed the highest pooled prevalence among caprine (32.0%, 95% CI: 17.0–52.0%), bovine (24.0%, 95% CI: 17.0–33.0%), porcine (17.0%, 95% CI: 7.0–36.0%) and avian species (13.0%, 95% CI: 9.0–19.0%). Animal-derived food products showed a pooled prevalence of 19.0% (95% CI: 17.0–21.0%). Regional analysis showed the highest pooled prevalence in South-West (19.0%, 95% CI: 13.0–27.0%) and South-South (19.0%, 95% CI: 9.0–34.0%). Studies using combined culture and molecular methods reported higher pooled prevalence (19.0%, 95% CI: 14.0–25.0%) than culture alone (12.0%, 95% CI: 8.0–18.0%). However, the difference between subgroups was not statistically significant (test for subgroup differences: p = 0.0563). Conclusions: These findings confirm extensive ESBL-producing E. coli circulation in Nigerian food-producing animals and highlight critical gaps in antimicrobial stewardship, veterinary surveillance, and food safety infrastructure, underscoring the urgent need for coordinated One Health strategies to contain the spread of resistant strains through the food chain. Full article

The genus Trichinella comprises zoonotic nematodes infecting a wide range of carnivorous and omnivorous animals, including humans. Infection occurs through the consumption of raw or undercooked meat containing viable Trichinella larvae. Among the species within this genus, Trichinella spiralis is considered one of the most epidemiologically important due to its high reproductive capacity and its frequent association with infections in domestic animals and humans. In this study, muscle samples from 18 invasive American minks (Neovison vison) were examined for Trichinella larvae using the magnetic stirrer method. Species identification was performed via multiplex polymerase chain reaction (PCR), while the internal transcribed spacer 1 (ITS1) region was amplified to evaluate the intraspecific genetic variability. Trichinella larvae were detected in one of the 18 (5.6%) animals investigated, and all isolates were identified as T. spiralis. Ten ITS1 sequences obtained from individual larvae were 100% identical. Network and principal coordinate analyses revealed that the sequences clustered by geographic origin rather than host species and were more related to isolates from domestic pigs than to wildlife animals. These findings provide the first evidence of T. spiralis in American minks in Baltic and Scandinavian countries and contribute to a better understanding of the epidemiology of trichinellosis in the region. Full article

In recent years, the escalation of international and internal conflicts in the Middle East and North Africa and Bulgaria’s geographical location, which places it on the route of those seeking protection from these regions in Europe, has led to a significant increase in the number of refugee children arriving in Bulgaria. According to Eurostat data, Bulgaria ranks fourth in terms of the number of unaccompanied refugee children in the European Union. In this article, the possibilities for achieving the welfare of refugee children and their successful integration into society are considered as a result of the intersection of two main trends—state legislation and public policies aimed at guaranteeing the best interests of refugee children, and public opinion about refugee children, which, in some cases, cause the failure of positive state measures and policies. The data on public attitudes towards refugee children used here are the result of an empirical study conducted in 2021 by the authors of this article. The results show that while state legislation and public policies are evolving towards ensuring the best interests of the child, the same cannot be said for public attitudes and the distance that the local population demonstrates towards refugee children. Full article

Background/Objectives: Estimate the prevalence of myopia among children aged 6–17 years in county and rural areas across seven geographically diverse provinces of China, and identify demographic, behavioral, and geographic factors associated with myopia, with particular focus on urban–rural and ethnic differences. Methods: A multi-stage stratified cluster sampling design was employed. Seven provinces were randomly selected, one from each of seven geographical regions of China (Southeast, North, Central, South, Southwest, Northwest, and Northeast). In each province, one rural county was randomly chosen. Within each county, one urban survey site (county town) and one rural survey site (village) were selected. From each site, one primary school and one junior high school were included. In each school, approximately 20 ± 2 students per grade (grades 1–9) were recruited. Uncorrected visual acuity and non-cycloplegic autorefraction were measured. Multivariable generalized linear mixed models (GLMM) with random intercepts at the class level were used to identify factors associated with myopia, accounting for the cluster sampling design. Results: The overall myopia prevalence was 42.9% (urban 49.6%, rural 36.0%). In the multivariable GLMM, educational stage was the strongest risk factor (grades 7–9 vs. 1–3: OR = 5.54). A significant district × ethnicity interaction was found only for Mongolian children: rural residence was strongly protective (OR = 0.19) compared to Han (OR = 0.65), and the ethnic advantage disappeared in county towns. Only 14.2% of myopic students had adequate correction. Conclusions: In conclusion, myopia is highly prevalent and severely under-corrected in rural China. Educational pressure is the main risk factor, and the rural protective effect is strongest in Mongolians but erodes with urbanization. Urgent public health actions, including vision screening, affordable spectacles, and lifestyle preservation, are needed to address this growing burden. Full article