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Search Results (218)

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Keywords = intergenerational change

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20 pages, 281 KB  
Review
The Youngest Minds in a Warming World: A Review of Climate Change and Child and Adolescent Mental Health
by Georgios Giannakopoulos
Psychiatry Int. 2025, 6(4), 119; https://doi.org/10.3390/psychiatryint6040119 - 2 Oct 2025
Abstract
Climate change poses a growing threat to the mental health of children and adolescents. This narrative review synthesizes global, interdisciplinary research on the psychological impacts of climate disruption during critical developmental stages, with attention to marginalized populations. We explore three key pathways of [...] Read more.
Climate change poses a growing threat to the mental health of children and adolescents. This narrative review synthesizes global, interdisciplinary research on the psychological impacts of climate disruption during critical developmental stages, with attention to marginalized populations. We explore three key pathways of harm: direct exposure to environmental disasters, chronic disruption of ecological and social systems, and existential distress such as eco-anxiety. Drawing on eco-social theory and developmental psychopathology, the review highlights how these impacts are shaped by age, geography, identity, and systemic inequities. It identifies both risk and protective factors, emphasizing the importance of caregiving relationships, cultural practices, education, and youth climate engagement. While activism can foster resilience and purpose, it may also incur emotional burdens that require clinical and policy attention. We argue that child and adolescent mental health must be recognized as central to climate justice and adaptation, and we offer urgent recommendations for integrated action across sectors. Full article
34 pages, 1369 KB  
Article
Intergenerational Differences in Impulse Purchasing in Live E-Commerce: A Multi-Dimensional Mechanism of the ASEAN Cross-Border Market
by Yanli Pei, Jie Zhu and Junwei Cao
J. Theor. Appl. Electron. Commer. Res. 2025, 20(4), 268; https://doi.org/10.3390/jtaer20040268 - 2 Oct 2025
Abstract
Existing research on live-streaming e-commerce consumption behavior is mostly limited by a single disciplinary framework, unable to systematically parse the mechanism of macro-policies and cultural values on intergenerational consumer psychology. This study takes ASEAN cross-border live-streaming e-commerce as a scenario, integrates theories of [...] Read more.
Existing research on live-streaming e-commerce consumption behavior is mostly limited by a single disciplinary framework, unable to systematically parse the mechanism of macro-policies and cultural values on intergenerational consumer psychology. This study takes ASEAN cross-border live-streaming e-commerce as a scenario, integrates theories of economics, political science, and sociology, and constructs an innovative three-layer analysis model of “macroeconomic system–meso-market–micro-behavior” based on multi-source data from 2020 to 2024. It empirically explores the formation mechanism of intergenerational differences in impulse buying. The results show that the behavior differences of different groups are significantly driven by income gradient, cross-border policies (tariff adjustment and consumer protection regulations), and collectivism/individualism cultural orientations. The innovative contribution of this study is reflected in three aspects: Firstly, it breaks through the limitation of a single discipline, and for the first time, it incorporates structural variables such as policy synergy effect and family structure change into the theoretical framework of impulse buying, quantifying and revealing the differentiated impact of institutional heterogeneity in ASEAN markets on intergenerational behavior. Secondly, it reconstructs the transmission path of “cultural values–family structure–intergenerational behavior” and finds that the inhibitory effect of collectivism on impulse buying tends to weaken with age. Thirdly, it proposes a “policy instrument–generational response” matching model and verifies the heterogeneous impact of the same policy (such as tariff reduction) on different generations. This study fills the gaps in related research and can provide empirical support for ASEAN enterprises to formulate stratified marketing strategies and for policymakers to optimize cross-border e-commerce regulation. which is of great significance to promote the sustainable development of the regional live-broadcast e-commerce ecology. Full article
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30 pages, 2577 KB  
Article
Indigenous Knowledge and Sustainable Management of Forest Resources in a Socio-Cultural Upheaval of the Okapi Wildlife Reserve Landscape in the Democratic Republic of the Congo
by Lucie Mugherwa Kasoki, Pyrus Flavien Ebouel Essouman, Charles Mumbere Musavandalo, Franck Robéan Wamba, Isaac Diansambu Makanua, Timothée Besisa Nguba, Krossy Mavakala, Jean-Pierre Mate Mweru, Samuel Christian Tsakem, Michel Babale, Francis Lelo Nzuzi and Baudouin Michel
Forests 2025, 16(10), 1523; https://doi.org/10.3390/f16101523 - 28 Sep 2025
Abstract
The Okapi Wildlife Reserve (OWR) in northeastern Democratic Republic of the Congo represents both a biodiversity hotspot and the ancestral homeland of the Indigenous Mbuti and Efe peoples, whose livelihoods and knowledge systems are closely tied to forest resources. This study investigates how [...] Read more.
The Okapi Wildlife Reserve (OWR) in northeastern Democratic Republic of the Congo represents both a biodiversity hotspot and the ancestral homeland of the Indigenous Mbuti and Efe peoples, whose livelihoods and knowledge systems are closely tied to forest resources. This study investigates how Indigenous knowledge and practices contribute to sustainable resource management under conditions of rapid socio-cultural transformation. A mixed-methods approach was applied, combining socio-demographic surveys (n = 80), focus group discussions, floristic inventories, and statistical analyses (ANOVA, logistic regressions, chi-square, MCA). Results show that hunting, fishing, gathering, and honey harvesting remain central livelihood activities, governed by customary taboos and restrictions that act as de facto ecological regulations. Agriculture, recently introduced through intercultural exchange with neighboring Bantu populations, complements rather than replaces traditional practices and demonstrates emerging agroecological hybridization. Nevertheless, evidence of biodiversity decline (including local disappearance of species such as Dioscorea spp.), erosion of intergenerational knowledge transmission, and increased reliance on monetary income indicate vulnerabilities. Multiple Correspondence Analysis revealed a highly structured socio-ecological gradient (98.5% variance explained; Cronbach’s α = 0.977), indicating that perceptions of environmental change are strongly coupled with demographic identity and livelihood strategies. Floristic inventories confirmed significant differences in species abundance across camps (ANOVA, p < 0.001), highlighting site-specific pressures and the protective effect of persistent customary norms. The findings underscore the resilience and adaptability of Indigenous Peoples but also their exposure to ecological and cultural disruptions. We conclude that formal recognition of Indigenous institutions and integration of their knowledge systems into co-management frameworks are essential to strengthen ecological resilience, secure Indigenous rights, and align conservation policies with global biodiversity and climate agendas. Full article
(This article belongs to the Special Issue Forest Ecosystem Services and Sustainable Management)
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26 pages, 12107 KB  
Article
Empowering Older Migrants: Co-Designing Climate Communication with Chinese Seniors in the UK
by Qing Ni, Hua Dong and Antonios Kaniadakis
J. Ageing Longev. 2025, 5(4), 37; https://doi.org/10.3390/jal5040037 - 24 Sep 2025
Viewed by 19
Abstract
This study explores how older Chinese migrants in London engage with climate change discourse using participatory co-design workshops. Although already practising sustainability behaviours such as recycling, this group faces significant barriers—particularly language difficulties and cultural differences—that limit their active participation in broader climate [...] Read more.
This study explores how older Chinese migrants in London engage with climate change discourse using participatory co-design workshops. Although already practising sustainability behaviours such as recycling, this group faces significant barriers—particularly language difficulties and cultural differences—that limit their active participation in broader climate initiatives. The research addresses three key aspects: (1) identifying opportunities for sustainable practices within migrants’ daily routines; (2) understanding their influential roles within families and communities; and (3) examining their trusted sources and preferred channels for climate communication. Results highlight that family and community networks, combined with digital platforms (e.g., WeChat) and visually engaging materials, play essential roles in disseminating climate information. Participants expressed strong motivations rooted in intergenerational responsibility and economic benefits. The findings emphasise the necessity of inclusive and peer-led communication strategies that are attuned to older migrants’ linguistic preferences, media habits, and cultural values—underscoring their significant but often overlooked potential to meaningfully contribute to climate action. Full article
(This article belongs to the Special Issue Aging in Place: Supporting Older People's Well-Being and Independence)
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20 pages, 1534 KB  
Article
Changing Attitudes Towards Retirement and Ageing Through Flipped Classroom and Collaborative Learning: A Social Psychological Study with Psychology and Social Work Students
by María Natividad Elvira-Zorzo and Maria Teresa Vega Rodríguez
Soc. Sci. 2025, 14(9), 562; https://doi.org/10.3390/socsci14090562 - 19 Sep 2025
Viewed by 370
Abstract
The phenomenon of population ageing indicates an overall improvement in health and quality of life. However, it also presents significant social challenges, particularly with regard to age discrimination and the negative stereotypes and attitudes towards older people known as ageism. Various studies have [...] Read more.
The phenomenon of population ageing indicates an overall improvement in health and quality of life. However, it also presents significant social challenges, particularly with regard to age discrimination and the negative stereotypes and attitudes towards older people known as ageism. Various studies have shown that these prejudices have a negative impact on the social integration and well-being of this group. This quasi-experimental pre-test–post-test study, which involved a non-equivalent comparison group, was carried out at the University of Salamanca with psychology and social work students. This study aimed to reduce negative stereotypes about old age and unfavourable attitudes towards retirement by implementing a three-phase educational programme. The programme incorporated flipped classroom methodology and intergenerational collaborative projects. Participants were divided into two groups: an intervention group responsible for designing intergenerational projects and a comparison group which did not participate in the intervention. The Negative Stereotypes towards Old Age Questionnaire (CENVE) and the Attitudes towards Retirement Scale (ARS) were administered before and after the programme. The results showed a significant decrease in negative stereotypes in the intervention group, with no changes observed in the comparison group. The impact varied according to academic discipline. Integrating active and intergenerational methodologies into educational contexts is proposed as a means of mitigating ageism, promoting inclusion, and fostering social justice. Full article
(This article belongs to the Section Family Studies)
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29 pages, 1962 KB  
Article
Supporting Sustainable Demographic Change: Augmented Reality in Men’s Sheds as a Concept for Active Aging
by Andrzej Wieczorek and Kinga Stecuła
Sustainability 2025, 17(18), 8345; https://doi.org/10.3390/su17188345 - 17 Sep 2025
Viewed by 345
Abstract
The rapid increase in the aging population presents significant challenges to sustainable development. This is particularly true in relation to the areas of social inclusion, healthcare, and lifelong learning. As societies try to adapt to demographic change, it is important to develop innovative [...] Read more.
The rapid increase in the aging population presents significant challenges to sustainable development. This is particularly true in relation to the areas of social inclusion, healthcare, and lifelong learning. As societies try to adapt to demographic change, it is important to develop innovative approaches that improve the quality of life of older adults while promoting their active participation in community life. Firstly, this paper examines current methods of activating seniors through social and technical engagement, with a particular focus on the concept of men’s sheds—a concept originating in Australia, in which a space where older adults men can socialize and engage in woodworking and other technical activities is offered. Despite the benefits of such initiatives, digital exclusion remains a key barrier that limits the full participation of seniors in a rapidly digitizing world. This paper explores the potential of integrating augmented reality (AR) into men’s sheds as a tool to limit digital gaps and support inclusive, active aging. The authors present a proposal and a practical implementation scenario for the use of AR in men’s sheds as a new concept to activate seniors. This paper examines the example of metal processing performed in men’s sheds using AR. The concept was then evaluated by experts. This paper presents the results of these experts’ opinions. The authors conducted interviews with seven experts. There were ten questions: seven closed-ended (rated on a scale from 1 to 5) and three open-ended questions. This study aligns with the concept of smart cities and the wider goals of sustainable development by promoting lifelong learning, intergenerational exchange, and improved well-being among seniors. Full article
(This article belongs to the Special Issue Demographic Change and Sustainable Development)
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17 pages, 1136 KB  
Article
“It’s Years of Walking, of Reading the Forest”: White Truffle Hunters’ Perception of Socio-Ecological Change in Langhe and Roero, NW Italy
by Mousaab Alrhmoun, Monica Zanaria, Federico Elia, Naji Sulaiman, Andrea Pieroni and Paolo Corvo
Sustainability 2025, 17(17), 8053; https://doi.org/10.3390/su17178053 - 7 Sep 2025
Viewed by 888
Abstract
Truffle hunting in the Piedmontese landscapes of Northern Italy is not merely a foraging practice but a deeply embodied and multispecies relationship grounded in intergenerational knowledge, sensory attunement, and emotional connection to forest ecologies. This study draws on qualitative interviews with local truffle [...] Read more.
Truffle hunting in the Piedmontese landscapes of Northern Italy is not merely a foraging practice but a deeply embodied and multispecies relationship grounded in intergenerational knowledge, sensory attunement, and emotional connection to forest ecologies. This study draws on qualitative interviews with local truffle hunters (Trifulau) to examine how socio-ecological transformations driven by land privatization, vineyard expansion, monocultural hazelnut plantations, and tourism disrupt these traditional practices. Thematic analysis reveals five dimensions of transformation: ecological estrangement, dispossession and exclusion, erosion of knowledge transmission, commodification and spectacularizing, emotional and ontological loss. Hunters describe a loss of sensory orientation, access to ancestral commons, and a breakdown of the human–dog forest relational web, accompanied by feelings of grief, alienation, and identity erosion. We argue that these changes undermine ecological sustainability and threaten emotional, cultural, and epistemological sustainability. The findings call for a broadened understanding of sustainability, one that recognizes affective, multispecies, and place-based knowledge systems as vital to sustaining cultural landscapes. This study contributes to debates on rural transformation, non-material heritage, and the invisible costs of commodifying traditional ecological practices in globalizing economies. Full article
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27 pages, 1573 KB  
Review
True Wealth of Nations: Valuing Resources Beyond GDP as a Framework for Sustainable and Inclusive Economic Policy in the European Union
by George Halkos, Panagiotis-Stavros C. Aslanidis and Shunsuke Managi
Economies 2025, 13(9), 257; https://doi.org/10.3390/economies13090257 - 5 Sep 2025
Viewed by 540
Abstract
Moving beyond Gross Domestic Product (GDP) as the sole measure of economic performance is increasingly critical for addressing the complex challenges of sustainable development. The Inclusive Wealth Index (IWI) offers a more comprehensive framework for assessing long-term sustainability by accounting for changes in [...] Read more.
Moving beyond Gross Domestic Product (GDP) as the sole measure of economic performance is increasingly critical for addressing the complex challenges of sustainable development. The Inclusive Wealth Index (IWI) offers a more comprehensive framework for assessing long-term sustainability by accounting for changes in produced, human, and natural capital. This paper contributes to this debate by examining the comparative dynamics of these three forms of capital in Greece in relation to European Union averages. Specifically, we employ a repeated-measures design and the mixed ANOVA method to analyse their interactions over time (1990–2020) and across regional contexts. The novelty is to cover the research gap on how the different capitals interact, with Greece serving as a critical case given its environmental vulnerabilities, economic challenges, and position within the European sustainability agenda. The empirical results demonstrate a consistent hierarchy (human > produced > natural), significant growth over time, and pronounced regional disparities, with Western and Northern Europe outperforming Eastern and Southern Europe in overall capital stocks. Moreover, human, produced, and natural capital differed significantly (ηp2=0.967), with the EU-27 dominated by human and produced capital, while Greece lagged substantially (ηp2=0.71). A robust interaction effect indicated structural divergence (ηp2=0.811). The pairwise comparisons confirmed these results with very large effect sizes (Cohen’s d = 2.3–11.2 in the 95% CI). These findings underscore the importance of moving beyond GDP and highlight the policy relevance of inclusive wealth accounting for ensuring resilience and intergenerational equity. Full article
(This article belongs to the Section Economic Development)
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20 pages, 1096 KB  
Article
Connect Active Programme (CAP): A Pilot RCT to Enhance Physical Activity and Intergenerational Relationships Through Dyadic Digital Walking Exercises
by Mimi Mun Yee Tse, Percy Poo-see Tse, Ka Yan Ip, Ho Yuen Lam, Pak San Chong, Tyrone Tai On Kwok, Grace Yuying Sun, Samuel Kai Wah Chu and Kin Pong To
Healthcare 2025, 13(16), 2043; https://doi.org/10.3390/healthcare13162043 - 19 Aug 2025
Viewed by 638
Abstract
Background/Objectives: Sedentariness is a common phenomenon among both the elderly and the young in modern society. Changes in work structure, leisure activities, and technological advancements have contributed to excessive sitting time. To address sedentary lifestyles across generations, this randomised controlled pilot trial [...] Read more.
Background/Objectives: Sedentariness is a common phenomenon among both the elderly and the young in modern society. Changes in work structure, leisure activities, and technological advancements have contributed to excessive sitting time. To address sedentary lifestyles across generations, this randomised controlled pilot trial aimed to investigate the effectiveness of the Connective Active Programme (CAP) in improving intergenerational relationships, psychological well-being, and physical fitness among older adults. Methods: Twenty dyads of older and younger adults from the same family were recruited and randomly allocated to an experimental group or a control group in a 1:1 ratio. The experimental group participated in six weekly 2-h dyadic walking sessions supported by digital information and mobile applications, whereas the control group received weekly digital pamphlets. Results: Compared to the control group, the experimental group showed significant improvements in younger participants’ intergenerational relationship quality, as well as in older participants’ 6-Minute Walk Test performance and WHOQOL-BREF scores. Conclusions: The CAP appears to be a viable strategy to enhance intergenerational relationships and promote healthy ageing among community-dwelling older adults in Hong Kong. Full article
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34 pages, 15665 KB  
Article
Integrating Aging-Friendly Strategies into Smart City Construction: Managing Vulnerability in Rural Mountainous Areas
by Kexin Chen, Yangyang Lei, Qian Liu, Jing’an Shao and Xinjun Yang
Buildings 2025, 15(16), 2885; https://doi.org/10.3390/buildings15162885 - 14 Aug 2025
Viewed by 342
Abstract
The vulnerability of older adults in rural mountainous regions presents a critical challenge for sustainable development, particularly in the context of smart city and digital town construction. In this study, we develop a comprehensive analytical framework and evaluation index to assess Vulnerability to [...] Read more.
The vulnerability of older adults in rural mountainous regions presents a critical challenge for sustainable development, particularly in the context of smart city and digital town construction. In this study, we develop a comprehensive analytical framework and evaluation index to assess Vulnerability to Elderly Poverty (VEP) and adaptive capacity, with a focus on its integration with smart infrastructure and age-friendly rural built environment strategies. Using Shizhu County in Chongqing, China, as a case study, we explore spatial disparities in VEP and apply quantile regression to identify the driving factors of adaptability. Our findings indicate that subsidy-dependent, middle-aged, and empty-nest older adults are the most vulnerable groups, with limited capacity to adapt to changing environments. A geographically alternating “high–low–high–low” VEP pattern reflects uneven development in infrastructure, accessibility, and public service construction. These disparities highlight the need for targeted planning and building interventions in rural settings. The key factors influencing adaptability include individual attributes, intergenerational support, and macro-level conditions such as policy design and digital infrastructure deployment. The integration of aging-friendly building strategies, smart infrastructure, and digital tools significantly enhances older adults’ resilience and social inclusion. Based on our results, we propose four adaptation models for aging populations in rural areas, emphasizing the construction of inclusive digital infrastructure, aging-sensitive building design, and community-based support systems. Strategic recommendations include promoting digital literacy through built environment interventions, enhancing intergenerational living arrangements, and embedding elderly-responsive features into smart construction planning. This research offers new insights into construction management practices that support aging in place and poverty alleviation through inclusive and resilient built environments. Full article
(This article belongs to the Section Architectural Design, Urban Science, and Real Estate)
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14 pages, 475 KB  
Article
Towards Intergenerational Sustainability: Extended Self-Interest and Older Adults’ Support for Childcare Policy
by Suk Eun
Sustainability 2025, 17(15), 6771; https://doi.org/10.3390/su17156771 - 25 Jul 2025
Viewed by 503
Abstract
(1) Background: This study investigates whether older adult individuals support childcare policies not only out of altruism, but also due to extended self-interest arising from intergenerational co-residence. It challenges the conventional view that welfare attitudes are shaped solely by one’s own life-cycle needs. [...] Read more.
(1) Background: This study investigates whether older adult individuals support childcare policies not only out of altruism, but also due to extended self-interest arising from intergenerational co-residence. It challenges the conventional view that welfare attitudes are shaped solely by one’s own life-cycle needs. (2) Methods: Using the 2013 and 2016 waves of the Korean Welfare Panel Study waves of the Korean Welfare Panel Study, a difference-in-differences (DiD) approach compares attitudes toward government childcare spending between older adults living with children (Co-residing Older Adults) and those who do not (Non-co-residing Older Adults), before and after universal childcare policies were introduced in 2013. (3) Results: The Co-residing Older Adults consistently expressed stronger support for family policies than their counterparts. However, this support did not significantly increase after the 2013 reform, indicating that extended self-interest may not be sensitive to short-term policy changes. (4) Conclusions: Extended self-interest appears to be a stable orientation shaped by family context rather than a flexible, policy-reactive stance. These findings highlight the role of intergenerational household ties in shaping welfare attitudes and offer implications for fostering generational solidarity in aging societies. Full article
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24 pages, 2698 KB  
Article
Modelling Nature Connectedness Within Environmental Systems: Human-Nature Relationships from 1800 to 2020 and Beyond
by Miles Richardson
Earth 2025, 6(3), 82; https://doi.org/10.3390/earth6030082 - 23 Jul 2025
Cited by 1 | Viewed by 16992
Abstract
Amid global environmental changes, urbanisation erodes nature connectedness, an important driver of pro-environmental behaviours and human well-being, exacerbating human-made risks like biodiversity loss and climate change. This study introduces a novel hybrid agent-based model (ABM), calibrated with historical urbanisation data, to explore how [...] Read more.
Amid global environmental changes, urbanisation erodes nature connectedness, an important driver of pro-environmental behaviours and human well-being, exacerbating human-made risks like biodiversity loss and climate change. This study introduces a novel hybrid agent-based model (ABM), calibrated with historical urbanisation data, to explore how urbanisation, opportunity and orientation to engage with nature, and intergenerational transmission have shaped nature connectedness over time. The model simulates historical trends (1800–2020) against target data, with projections extending to 2125. The ABM revealed a significant nature connectedness decline with excellent fit to the target data, derived from nature word use in cultural products. Although a lifetime ‘extinction of experience’ mechanism refined the fit, intergenerational transmission emerged as the dominant driver—supporting a socio-ecological tipping point in human–nature disconnection. Even with transformative interventions like dramatic urban greening and enhanced nature engagement, projections suggest a persistent disconnection from nature through to 2050, highlighting locked-in risks to environmental stewardship. After 2050, the most transformative interventions trigger a self-sustaining recovery, highlighting the need for sustained, systemic policies that embed nature connectedness into urban planning and education. Full article
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10 pages, 237 KB  
Article
Experiencing Old Age: Pilot Study Examining the Effects of Age Simulation on Ageism in Adolescents
by Nadja Vesic and Nemanja Rancic
Adolescents 2025, 5(3), 34; https://doi.org/10.3390/adolescents5030034 - 11 Jul 2025
Viewed by 451
Abstract
Background: As global populations age, addressing societal attitudes towards older adults becomes increasingly crucial. Ageism negatively impacts both older and younger individuals. Aging simulation has been proposed as a means to challenge ageist attitudes and promote intergenerational solidarity. This study examines the effectiveness [...] Read more.
Background: As global populations age, addressing societal attitudes towards older adults becomes increasingly crucial. Ageism negatively impacts both older and younger individuals. Aging simulation has been proposed as a means to challenge ageist attitudes and promote intergenerational solidarity. This study examines the effectiveness of a brief aging simulation in altering adolescents’ perceptions of aging and older adults. Methods: A prospective interventional study was conducted with 63 high school students aged 15–18. Participants completed the Fraboni Scale of Ageism (FSA) before and after intervention. A subset of 20 students was randomly selected to wear an aging simulation suit. Results: Post-intervention FSA scores significantly decreased from 63.50 to 51.00, indicating a reduction in ageist attitudes. Participants showed significant changes in beliefs related to cognitive decline, the prevalence of Alzheimer’s disease, and the perception that older adults are homogeneous. Additionally, 80% of participants reported a positive shift in their attitude toward aging. The simulation experience was rated highly (average score: 9/10). Conclusions: The findings support the effectiveness of aging simulation in reducing ageist attitudes among adolescents. Given the role of adolescence in shaping long-term perceptions, incorporating aging simulations into educational curricula could be a valuable strategy for promoting intergenerational understanding. Full article
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21 pages, 4028 KB  
Article
The Response Characteristics of One Saccharomyces cerevisiae Strain Under Continuous Passage in Artificial Culture Medium
by Tengyu Ma, Hongguang Zhu, Jiajia Yin, Yu Tian, Wenjing Yan and Haixin Sun
J. Fungi 2025, 11(7), 513; https://doi.org/10.3390/jof11070513 - 9 Jul 2025
Viewed by 764
Abstract
Saccharomyces cerevisiae often undergoes strain degeneration during industrial serial subculturing, though this phenomenon remains understudied. This study first conducted strain screening and biological characterization through TTC (2,3,5-triphenyltetrazolium chloride) colorimetric assays, Durham tube fermentation gas production tests, and WL medium (Wallerstein Laboratory medium) cultivation. [...] Read more.
Saccharomyces cerevisiae often undergoes strain degeneration during industrial serial subculturing, though this phenomenon remains understudied. This study first conducted strain screening and biological characterization through TTC (2,3,5-triphenyltetrazolium chloride) colorimetric assays, Durham tube fermentation gas production tests, and WL medium (Wallerstein Laboratory medium) cultivation. Subsequently, the changes in intergenerational biological traits after serial subculturing were investigated. Finally, transcriptomic analysis was employed to examine differential gene expression under high-glucose stress during continuous subculturing. The experimental results demonstrated that: (1) The S. cerevisiae QDSK310-Z-07 (GenBank: PP663884), isolated from farm soil, exhibited robust growth within a temperature range of 24–36 °C, with optimal growth observed at 28 °C. It thrived in a pH range of 4–5.5 and efficiently utilized various carbon and nitrogen sources; (2) After serial subculturing, the strain’s ethanol production capacity and fermentation rate partially declined and then stabilized, while maintaining strong tolerance to high ethanol concentrations and hyperosmotic stress; (3) Transcriptomic analysis revealed significant differential expression of genes related to lipid metabolism, amino acid metabolism, and other pathways under high-glucose stress following continuous subculturing. These findings elucidate the biological trait variations in S. cerevisiae during serial subculturing and provide key metabolic regulation candidate targets for its long-term adaptive evolution under high-glucose stress. Full article
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21 pages, 1126 KB  
Article
Applying the 7P Framework to Youth–Adult Partnerships in Climate Organizing Spaces: “If We Are Going to Be the Ones Living with Climate Change, We Should Have a Say”
by Ellen Field and Lilian Barraclough
Youth 2025, 5(3), 66; https://doi.org/10.3390/youth5030066 - 3 Jul 2025
Viewed by 876
Abstract
Young people are frustrated and disheartened with the lack of adult leadership and action to address the climate crisis. Although youth representation in global, regional, and local decision-making contexts on climate change is steadily growing, the desired role and effect of youth in [...] Read more.
Young people are frustrated and disheartened with the lack of adult leadership and action to address the climate crisis. Although youth representation in global, regional, and local decision-making contexts on climate change is steadily growing, the desired role and effect of youth in environmental and climate decision-making has shifted from a focus on having youth voices heard, to having a direct and meaningful impact on policy and action. To meaningfully integrate youth perspectives into climate policies and programs, intergenerational approaches and youth–adult partnerships are key. This paper explores strategies to support youth action and engagement as adult partners by investigating youth perspectives on what adults and adult-led organizations should consider when engaging young people in climate-related work. This qualitative research study introduces a revised version of the 7P youth participation framework, developed through focus groups with high school youth. This paper provides reflective questions and practical recommendations for participants engaged in youth–adult partnerships to help guide engagement beyond token representation and create meaningfully participatory conditions for youth agency in climate organizing spaces. Full article
(This article belongs to the Special Issue Politics of Disruption: Youth Climate Activisms and Education)
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