Search Results (819)

In this study, the effects of five bean seeds (Vigna radiata, Pisum sativum, Vigna unguiculata, Vicia faba, and Glycine max) on the development, survival, oviposition, and digestive enzyme activities of C. chinensis were investigated. The duration of C. chinensis development from egg to adult was shortest when reared on V. radiata (26.97 d) and longest on G. max (30.92 d) (p < 0.01). Similarly, the emergence rate of C. chinensis was highest on V. radiata (66.11%) and lowest on G. max (33.89%) (p < 0.01). Significant differences in the fecundity of C. chinensis were also detected, with the highest values on V. radiata (63.36 eggs/female), followed by P. sativum (54.27 eggs/female), V. unguiculata (51.30 eggs/female), V. faba (42.47 eggs/female), and G. max (37.29 eggs/female) (p < 0.01). For an equal initial insect number, after continuous rearing for 30, 60, or 90 d, there were significantly more offspring on V. radiata than on the other species, with the fewest offspring detected on G. max (p < 0.01). Although CL activity (p = 0.33) in C. chinensis did not differ significantly among the different legume species, activities of PEP (p < 0.01) and α-AMS (p < 0.01) in C. chinensis were all highest on V. radiata and lowest on G. max, and activities of LPS (p < 0.01) were highest on G. max and lowest on V. radiata. The differences in population dynamics associated with different bean seeds may be related to the activity of particular digestive enzymes, which play important roles in insect nutrient metabolism and growth. Our results indicated that V. radiata is the most suitable host diet, whereas G. max is the least suitable for the development of C. chinensis. Full article

Leucaena leucocephala is a nitrogen-fixing legume widely used in agroforestry systems, although its invasive potential poses increasing risks to wetlands and riparian ecosystems. This systematic review synthesizes current knowledge on the ecological mechanisms, environmental stressors, and management strategies associated with the invasion of L. leucocephala in humid tropical environments. Following PRISMA guidelines, 60 studies retrieved from Scopus, Web of Science, and Consensus were qualitatively analyzed. The results indicate that invasion success is strongly associated with environmental disturbances and stress conditions, particularly drought stress, altered hydrological regimes, fire occurrence, and land-use change, which reduce ecosystem resistance and facilitate species establishment. Key invasion mechanisms include high seed production, persistent soil seed banks, rapid growth, allelopathic effects, and strong resprouting capacity, leading to suppression of native vegetation and structural simplification of plant communities. Integrated management strategies combining mechanical and chemical control with active revegetation consistently showed higher effectiveness than isolated approaches. The evidence further suggests that climate-related stressors may intensify invasion dynamics and increase ecosystem vulnerability under future climate scenarios. Despite recent advances, important knowledge gaps remain regarding long-term ecosystem functioning, hydrological feedback, and adaptive management in invaded wetlands. Full article

The co-occurrence of mucilage and phenolic compounds within the same secretory cell is rarely documented in plants. Recently, such cells were reported in vegetative and floral organs of sensitive legumes (Mimosa), but without detailed subcellular analysis. To address this gap, we used transmission electron microscopy to examine the organelles involved in biosynthesis, the intracellular sites of metabolite storage, and the secretion processes across floral and foliar organs in five Mimosa species. Secretory epidermal cells of sepals, petals, and leaf blades produce both mucilage and phenolics, with no significant differences between organ types. Dictyosomes, rough endoplasmic reticulum, and plastids predominated in the cytoplasm of the secretory cell during biosynthesis. Dictyosomes may mediate mucilage production, the rough endoplasmic reticulum may be involved in phenolic synthesis, and plastids may contribute to the biosynthesis of both compounds. These metabolites are stored in distinct cellular domains: phenolics accumulate in a large vacuole near the outer periclinal wall, while mucilage is deposited between the microfibrils of the inner periclinal wall. This spatial separation is evident by the distention of the inner periclinal wall due to mucilage accumulation. The absence of karyokinesis and phragmoplast formation during metabolite segregation confirms that these secretory cells have two different functional domains, forming a uniseriate rather than biseriate epidermis. Notably, the inclusion of several species in the ultrastructural analyses enhances the significance of these findings. Full article

Phytoestrogens are plant-derived phenolic compounds that structurally resemble endogenous estrogens and can exert both estrogenic and anti-estrogenic effects in animals. In ruminant nutrition, the main classes of phytoestrogens (isoflavones, lignans, stilbenes, coumestans and selected flavonoids) are supplied predominantly by legume forages and soybean-based feeds, in which concentrations can reach several mg/g of dry matter. After ingestion, these compounds are extensively metabolized by the rumen microbiota to derivatives with altered biological potency, such as equol and p-ethyl-phenol, which influence endocrine, immune and metabolic pathways. Experimental and field studies in cattle, sheep and goats indicate that dietary phytoestrogens may improve nitrogen utilization, immune competence, growth performance, antioxidant status and milk yield. However, they can also impair fertility, modify hormone profiles and compromise embryo survival in a compound-, dose-, and species-dependent manner. In this review, we summarize current knowledge on the botanical and nutritional sources, ruminal metabolism and transfer of phytoestrogens in ruminants, and critically examine their effects on blood metabolites, immune responses, growth and carcass traits and lactational performance and reproductive function. A structured literature search based on PRISMA principles was used to identify and appraise experimental and observational studies in both grazing and intensive production systems up to 2025. Remaining knowledge gaps and practical implications for the safe use of phytoestrogen-rich feeds in livestock production are highlighted. Full article

Agroforestry and perennial tree crop production systems, particularly in Mediterranean regions, exhibit a high degree of integration among trees, herbaceous, and soil components. They provide essential services including provisioning, regulation, support, and cultural services, which enhance human health, well-being, and economic stability. However, guaranteeing their long-term resilience in the face of environmental challenges, including drought and soil degradation, is essential for the sustainable management of these systems. We examine the impact of microsite conditions (soil and herbaceous layer) and their management on olive trees (Olea europaea L.) under varying levels of drought stress. A fully factorial design was implemented in a Spanish agroforestry system, combining two irrigation regimes (rainfed vs. summer irrigation) and two soil management practices (customary plowing vs. herbaceous layer conservation) across four independent and replicated zones. Twelve olive trees per zone were individually monitored, treating each tree as the experimental unit, with one 50 × 50 cm sampling plot per tree in which microsite conditions were characterized for each tree. Plowed areas (shallow tillage) showed lower industrial extraction yield (%), fat yield based on dry matter (%), olive maturity and phytosanitary status compared to areas conserving their herbaceous layer cover (0.81, 0.96, 0.92, and 0.65-fold lower, respectively). Rainfed areas (i.e., those without supplemental water supply) showed a reduction in both industrial extraction yield (%), olive yield (kg tree⁻¹) and oil yield (kg ha⁻¹) (0.77, 0.86 and 0.67-fold lower, respectively). Under combined tillage and water-deficit conditions, oil yield (kg ha⁻¹), industrial extraction yield (%), and total phenolic content (ppm) were considerably lower (0.50, 0.60, and 0.67-fold lower, respectively). Furthermore, low quality of the herbaceous layer dominated by nitrophilous invasive species were associated with decreased leaf nutrient content, lower industrial extraction yield, reduced olive maturity and poorer phytosanitary status of olives. These findings suggest that maintaining a spontaneous herbaceous layer with a high-quality species (legume incorporation) and well-managed herbaceous cover, i.e., repeated mowing of the herbaceous layer instead of customary plowing, can enhance sustainable olive production by improving soil resilience, reducing water stress, and optimizing nutrient use, thereby supporting long-term ecosystem stability and agricultural productivity. Full article

The benefits of legume-nitrogen-fixing bacteria symbioses are vital in agricultural systems globally. Cross-infectivity studies are important for identifying rhizobial strains with potential for use as inoculants. The native rhizobial isolates inoculated on different legume species are the first step to determining host range and ecological adaptive traits. This study reports on the water-use efficiency and mineral nutrition of diverse legume species cross-inoculated by native rhizobial isolates from Eswatini, Ghana and South Africa under glasshouse conditions. A portable infrared red gas analyzer was used for water use efficiency. Data from a gas exchange study shows that rhizobial strains can significantly influence the photosynthetic functioning of their host plants. As a result, photosynthetic rates differed depending on bacterial compatibility with the host plant, as well as its symbiotic efficacy. Isolate TUTGmGH2 induced greater accumulation of P, K, Mg, Zn, Cu and Mn in soybean and Winged bean, clearly suggesting that rhizobia do have an influence on the mineral nutrition of their host plants. Therefore, these findings further show that native rhizobial isolates can be manipulated to enhance mineral nutrient uptake, promote growth and development and also produce nutrient-dense food with a low environmental impact globally since rhizobia do have an influence on the mineral nutrition of their host plants. Full article

Legume–grass mixtures are widely used to improve productivity and soil quality in alpine grasslands; however, their effects on soil fungal communities and the underlying mechanisms remain unclear. In this study, a two-factor field experiment (species richness (three, four, and five species) × legume–grass ratio (4:6, 3:7 and 2:8)) was conducted in an alpine artificial grassland on the Qinghai–Tibetan Plateau. Soil fungal communities were assessed using high-throughput sequencing combined with multivariate analyses. The results showed that Ascomycota, Mortierellomycota, and Basidiomycota were the dominant fungal phyla. Both species composition and seeding ratio significantly influenced fungal community structure and α-diversity primarily through indirect pathways mediated by plant community characteristics and soil properties. Plant height and soil total phosphorus (TP) were identified as key drivers of fungal α-diversity. Specifically, the four-species mixture (Z2) at a 3:7 legume–grass ratio resulted in relatively higher and more stable aboveground biomass and improved soil nutrient status, whereas increasing species richness to five species did not further enhance these benefits. Overall, our findings indicate that optimizing species composition and legume–grass ratio, rather than simply increasing species richness, is more effective for regulating soil fungal diversity and ecosystem function. Full article

In the context of sustainable agriculture and the need to reduce mineral nitrogen inputs, intercropping cereals with legumes is increasingly considered a promising strategy to enhance nutrient use efficiency and improve feed quality. However, the effects of such systems, combined with varying nitrogen fertilization levels, on the dynamics of micronutrients in soil and plant biomass remain insufficiently explored. Field research was conducted in central Poland, in Ciechanów, from 2021 to 2023, during the months of April through July each year. The aim of the study was to analyze the impact of intercropping spring barley and spring triticale with narrowleaf lupin and varying mineral nitrogen fertilization (0–60 kg N ha⁻¹) on the concentration and uptake of Mn, Cu, Zn, and Fe in the soil and green matter intended for fodder. It was shown that both the sowing pattern and the level of N fertilization significantly differentiated the concentration of microelements in the soil and their concentration and uptake with the yield. As the proportion of lupine in the mixture increased, the post-harvest soil showed higher concentrations of Mn (2–8%), Cu (2–9%), Zn (9–33%), and Fe (4–10%), accompanied by a marked increase in their levels in green matter, ranging from 6% to 94% depending on the micronutrient. The highest uptake of micronutrients was obtained in intercropping systems with a predominance of legumes, especially with moderate fertilization (40–60 kg N ha⁻¹), where the growth ranged from 16% to as much as 139%. Compared to single-species crops, the intercropping system was characterized by higher efficiency of soil resource use and better mineral quality of the feed. The results indicate that the integration of legumes with cereals can be an effective tool for improving feed security while reducing the intensity of mineral fertilization, in line with the principles of sustainable agriculture. Full article

There is growing interest in vegan and vegetarian (veg*n) diets for dogs and cats, due to factors including pet health, environmental sustainability and farmed animal welfare. Such diets should be carefully designed and manufactured in order to be nutritionally sound. Digestibility is a key, although not the only, determinant of this, and it has sometimes been claimed that dogs and cats cannot effectively digest and utilize plant-based proteins. To evaluate this claim, studies assessing canine and/or feline digestibility of veg*n diets and ingredients were analyzed. Thirty-one studies were included: 22 specific to dogs, two specific to cats, and seven applicable to both species. Across various study designs, populations, digestibility metrics, dietary ingredients and processing methods, digestibility values of veg*n diets were consistently high and broadly comparable to those of conventional meat-based diets. In all five studies that assessed apparent total tract digestibility (ATTD) of veg*n pet diets, ATTD values exceeded 80% (dry matter), 85% (organic matter), 80% (crude protein), 89% (fat), 88% (nitrogen-free extract), and 86% (energy). These studies also indicate that individual vegan protein sources can be well digested by dogs and/or cats, including those derived from legumes (such as soy-derived ingredients), pulses, grains and microbial fermentation. Discrepancies exist regarding whether these ingredients are more, less or equally digestible compared to animal-based alternatives. Nevertheless, even in studies where vegan protein sources showed lower digestibility for specific nutrients, overall digestibility remained high. These findings support the use of nutritionally sound veg*n pet diets. Such diets are not normally significantly less digestible than conventional meat-based diets. Full article

Soybean is a globally important legume crop which fulfills most of its nitrogen (N) requirement through Biological Nitrogen Fixation (BNF) in symbiosis with Bradyrhizobium species, thereby reducing dependence on synthetic fertilizers and supporting more sustainable production systems. This review synthesizes current knowledge on the mechanism, capacity, and regulation of BNF in soybean, including nodule formation, nitrogenase activity and response to soil and environmental conditions. The evidence shows that BNF can provide a substantial share of the crop’s N uptake, although high-yielding systems frequently experience the “N gap”, which is a difference between a higher crop demand and a lower N supplied from BNF and existing soil reserves. This can be partially managed with strategies like inoculation, co-inoculation, re-inoculation or judicial application of N. This review further highlights the advances in microbial inoculant technologies, plant growth-promoting rhizobacteria (PGPR), soybean breeding and genetic engineering aimed at improving BNF stability, efficiency and capacity across different soil environments. Overall, the maximization of soybean BNF has strong potential to reduce synthetic fertilizer use, improve yield and seed quality, and enhance the economic and environmental sustainability of soybean-based systems. Full article

Pea (Pisum sativum L.) is an important temperate grain legume crop of high nutritional and agronomic value. Ascochyta blight, caused by a multi-species complex of necrotrophic fungi, remains a major constraint for pea production worldwide. This review synthesizes the available genetic, physiological and molecular knowledge on the pea–Ascochyta blight pathosystem, with emphasis on the genetic architecture of resistance, host defense mechanisms and the recent contributions from the omics disciplines. Current evidence indicates that genetic resistance to the various Ascochyta blight pathogens is incomplete and multicomponent, being associated with loci of small to moderate effect, with expression depending on organ, developmental stage and environment. Under field conditions, the observed phenotypes reflect the interaction between physiological resistance, plant architecture, phenology, canopy microenvironment and epidemic dynamics. Together, these factors bias phenotyping and limit the transferability of molecular markers. The practical value of these markers for use in marker-assisted selection (MAS) and genomic selection (GS) is presented and critically discussed. Future progress in breeding for Ascochyta blight resistance will depend on integrating molecular knowledge with a careful definition of ideotypes, well-calibrated phenotyping and multi-environment validation. Full article

Nutritional strategies for reducing greenhouse gases that negatively impact climate change have been investigated in recent years. Secondary compounds such as tannins are found in tree legumes, which have forage potential and the ability to reduce enteric methane emissions. The aim of this research is to evaluate the effect of forage legumes as a tannin source on enteric methane mitigation and rumen fermentation. The species studied were Acacia dealbata, Acacia melanoxylon, Albizzia lophantha, Lupinus pubescens, Inga insignis, Senna multiglandulosa, and Tecoma stans. The range of crude protein content in all species was variable. The neutral detergent fiber content was much higher in I. insignes, while S. multiglandulosa and L. pubescens had a lower content of acid detergent fiber and lignin detergent acid. I. insignes presented a statistically different lower gas production when compared with the other species subjected to in vitro fermentation. The species that presented the greatest potential for the reduction in enteric methane produced were I. insignes and A. melanoxylon. Additionally, a significant variation was evidenced in the pH of the inocula at 24 h of fermentation in a range of 6.82–7.25. S. multiglandulosa presented concentrations for acetate, propionate, and butyrate that differed significantly compared to the other species. Similarly, the highest concentration of NH₃-N was for S. multiglandulosa. However, the highest EDDM_4% and IVDDM were for I. insignes with 381.28 and 791.46 g/kg, respectively. it can be concluded that forages (e.g., I. insignes) with a higher content of condensed tannins favor rumen digestion and fermentation, probably increasing microbial protein synthesis and thereby reducing ruminal gas and methane production. Full article

Sugarcane is the leading feedstock for bioethanol in Brazil and worldwide, but its continuous cultivation can degrade soil through nutrient depletion and compaction. Integrating green manures such as Crotalaria and pigeon pea into rotations offers a sustainable way to improve soil structure, water infiltration, and nutrient cycling. When combined with sweet sorghum as a complementary crop, these species can mitigate soil physical constraints and strengthen the resilience of sugar–energy systems under rainfed conditions. This three-year field experiment evaluated the effects of green manure and sweet sorghum rotations on sugarcane yield and sandy-soil physical attributes. The treatments were arranged in a 3 × 2 factorial design with randomized blocks, including two green manures (Crotalaria and pigeon pea) and a fallow control, each combined with or without sweet sorghum rotation. Biometric traits and yields were measured for all crops, and soil physical properties were assessed after the sugarcane cycle. Green manure significantly increased the stalk yield and dry matter of both sweet sorghum and sugarcane. In sugarcane, rotations with Crotalaria and pigeon pea enhanced stalk and dry matter yields by up to 18%, while the highest increase (31%) occurred under the sweet sorghum rotation. Furthermore, green manures improved sandy-soil water retention, increased infiltration rates, and reduced penetration resistance. These results demonstrate that legume–sorghum rotations are an effective and low-input strategy to enhance crop yield and sandy-soil physical properties, contributing to more sustainable bioenergy production under tropical rainfed conditions. Full article

Summer cover crops can improve soil fertility and contribute to nitrogen (N) supply in temperate cropping systems, yet the effects of mixture composition and sowing timing remain insufficiently documented. This study evaluated biomass production and N accumulation of five multispecies cover crop mixtures grown in Estonia during 2024–2025 under two sowing dates per year. Aboveground biomass, botanical composition, and carbon (C) and nitrogen concentrations were measured to assess productivity, species contributions, and residue quality. Earlier sowing was generally associated with higher biomass and N accumulation, with first-sown mixtures producing, on average, 38.7% more biomass than later-sown mixtures. Mixture performance was strongly shaped by species composition and competitive hierarchies. Total N accumulation of the cover crop mixtures ranged from 42 to 275 kg N ha⁻¹ depending on mixture composition and sowing time, with mixtures dominated by common vetch (Vicia sativa L.) achieving the highest values. Oat (Avena sativa) dominated and contributed substantially to biomass in mixtures lacking competitive legumes, whereas sunflower (Helianthus annuus) and maize (Zea mays) performed less well under delayed sowing. Low-growing species such as Persian clover (Trifolium resupinatum) produced little biomass when grown with highly competitive species. Legumes exhibited lower C:N ratios than non-legumes, while mixture-level values remained moderate, suggesting residue quality with potential for favourable decomposition and nutrient release in summer cover crop systems under temperate conditions. Full article

Insect frass fertilisers (IFFs) are increasingly promoted as sustainable soil amendments within circular agricultural systems. However, the compatibility of IFFs with nitrogen-fixing legumes is poorly understood. This study evaluated the effects of a fertiliser produced from Hermetia illucens frass (HexaFrass™; HF) on germination, seedling emergence, shoot growth, and root nodulation in six forage legume species (Trifolium repens L., T. pratense L., T. incarnatum L., T. hybridum L., Melilotus albus Medik., and Medicago lupulina L.). Aqueous HF extracts (1% w/v) had no significant effect on seed germination, whereas higher concentrations (10% w/v) reduced germination in both T. pratense and T. incarnatum. In glasshouse trials, incorporation of HF (3 g per pot) did not affect seedling emergence but significantly increased shoot biomass across all plant species tested, with growth responses comparable to, or exceeding, those obtained with an equivalent mass of organic chicken manure. Across species, the shoot dry weight of the HF-treated plants was over nine times that obtained in the unfertilised control plants. Plant responses to HF application rate were non-linear, with maximum shoot biomass achieved at intermediate doses (~4–5 g per pot). Root nodulation exhibited a similar dose-dependent pattern: low HF application rates slightly enhanced nodulation, whereas higher rates suppressed nodule numbers. These findings indicate that IFFs can promote early growth of forage legumes, but reinforce that for each plant system (plant species, growing conditions, growing medium etc) there is a need to optimise fertiliser application rates to balance nutrient supply while avoiding the inhibitory effects observed at high rates. Further work is needed to establish the compatibility of IFFs with forage legumes in long-term trials performed under field conditions. Full article