Search Results (1,776)

Background: Digital health (mHealth) interventions are increasingly integrated into maternity care to improve health literacy and reassure expectant mothers. However, the “double-edged sword” of continuous monitoring may be associated with heightened anxiety. This study aimed to describe mHealth usage patterns and investigate the association between technology engagement and mental health outcomes among pregnant women in Romania, where perinatal distress is a significant public health challenge. Methods: This observational, cross-sectional study included 100 pregnant and immediate postpartum women at a tertiary maternity unit in Romania. Participants were stratified into mHealth Users (n = 52) and Non-Users (n = 48). Validated instruments, including the PHQ-9, GAD-7, and EPDS, assessed depressive and anxiety symptoms. Predictors of adoption were identified using multivariable binary logistic regression. Results: mHealth users were predominantly from urban environments (80.8% vs. 54.2%, p = 0.004) and reported higher rates of daily physical activity (p < 0.001). Users experienced significantly higher median scores for depression (PHQ-9: 6 vs. 4, p = 0.047), generalized anxiety (GAD-7: 7 vs. 6, p = 0.015), and pregnancy-specific anxiety (35 vs. 29.5, p = 0.028) compared to non-users. In the multivariable model, high psychological distress (OR 0.08 for low-stress vs. high-stress, p = 0.009) and urban residency (p = 0.043) were independent predictors of mHealth adoption. Notably, 96.2% of users shared their digital data with healthcare providers. Conclusions: mHealth adoption in this population is characterized by a “paradox of monitoring,” where usage is strongly associated with pre-existing psychological vulnerability and associated with higher distress. While these tools serve as markers for mental health risk, the high rate of data sharing offers a clinical opportunity for a hybrid model of care. Obstetricians should view high digital engagement as a prompt for targeted mental health screening and proactively mediate patient-generated data to mitigate anxiety. Full article

Chronic kidney disease (CKD) is associated with a markedly increased risk of cardiovascular (CV) morbidity and mortality that cannot be fully explained by traditional risk factors. Emerging evidence highlights the central role of the gut–kidney–heart axis, whereby gut microbiota dysbiosis promotes the generation and systemic accumulation of uremic toxins, including indoxyl sulfate (IS), p-cresyl sulfate (PCS), and trimethylamine N-oxide (TMAO). These metabolites contribute to endothelial dysfunction, oxidative stress, inflammation, and vascular remodeling, thereby accelerating CV disease progression in CKD. Dietary patterns represent a key modifiable factor influencing gut microbiota composition and metabolic activity. The Mediterranean diet, characterized by high intake of plant-based foods, dietary fiber, and polyphenols, and low consumption of red and processed meats, has emerged as a promising microbiota-targeted strategy. It promotes saccharolytic fermentation, enhances short-chain fatty acid production, and reduces proteolytic pathways responsible for uremic toxin generation. Accumulating evidence from observational studies, meta-analyses, and dietary intervention trials suggests that adherence to Mediterranean and plant-based dietary patterns is associated with reduced uremic toxin burden, improved renal outcomes, and lower CV risk in CKD populations. However, direct interventional evidence linking Mediterranean diet adherence to changes in specific uremic toxin levels remains limited. This narrative review summarizes current evidence on diet–microbiota interactions in CKD and highlights the Mediterranean diet as a biologically plausible strategy for targeting the gut–kidney–heart axis. Future well-designed randomized controlled trials (RCTs) are needed to confirm causal relationships and support clinical implementation. Full article

Cadmium (Cd) is a typical heavy metal pollutant in aquatic environments. It enters fish through the gills, digestive tract, and body surface, and accumulates mainly in the liver and kidneys, with species- and tissue-specific distribution. Cadmium triggers apoptosis by inducing oxidative stress, calcium imbalance, and DNA damage. These signals are integrated and amplified by the mitogen-activated protein kinase (MAPK), nuclear factor kappa B (NF-κB), phosphatidylinositol 3-kinase (PI3K)/AKT, and nuclear factor erythroid 2-related factor 2 (Nrf2) pathways, ultimately activating three downstream apoptotic execution pathways: the death receptor, mitochondrial, and endoplasmic reticulum stress pathways. These three pathways form an interactive network through molecular nodes such as BH3 interacting domain death agonist (Bid), Ca²⁺, c-Jun N-terminal kinase (JNK), and C/EBP homologous protein (CHOP), synergistically amplifying the apoptotic effect, with the mitochondrial pathway playing a central role. Cadmium-induced apoptosis is dose-dependent: low concentrations activate protective responses, whereas high concentrations strongly promote apoptosis. Current research gaps remain regarding dynamic pathway crosstalk, chronic low-dose effects, species differences, and fish-specific apoptotic molecules (e.g., caspase-12 homologs). Future studies should focus on constructing multidimensional response maps, clarifying pathway activation thresholds and interaction contributions, and developing composite protective strategies based on Nrf2 activators, metal chelators, and antioxidants, thereby promoting translation into ecological risk assessment and aquaculture pollution control. Full article

Background: Early-onset Alzheimer’s disease (EOAD) caused by autosomal dominant mutations provides a deterministic framework for investigating genetic modifiers of neurodegeneration. Telomere biology has emerged as a central regulator of genomic stability, cellular ageing, and stress response integration, yet its role in EOAD, particularly in under-represented populations, remains poorly defined. Methods: We conducted a cross-sectional case–control study to evaluate the genetic distribution, disease association, and predicted regulatory consequences of common variants in the telomere maintenance genes TERT and TERC in individuals from Western Mexico. The EOAD group comprised genetically confirmed carriers of the PSEN1 p.Ala431Glu (A431E) founder mutation with clinical EOAD (n = 69), and controls were unrelated individuals without dementia (n = 179). Five common variants were analyzed: rs2242652, rs2853677, rs2736100, and rs10069690 (TERT), and rs12696304 (TERC). Results: Genotype distributions in controls conformed to the Hardy–Weinberg equilibrium. Single-variant analyses showed no significant allele-level associations. Most TERT variants did not show significant allele-level associations with EOAD. However, a preliminary genotype-level enrichment for the GC allele at rs12696304 (TERC) was observed among EOAD cases compared with controls; allele-level associations were not significant. Linkage disequilibrium analysis revealed low r² values (<0.20), supporting variant independence. Population-level allele frequency comparisons revealed ancestry-dependent divergence across loci; in silico functional annotation localised all variants to non-coding regulatory regions. GTEx-based analyses indicated that rs12696304 acts as an eQTL for ACTRT3 in whole blood and pituitary, as well as for LRRC34 in the cerebellar hemisphere, suggesting a potential regulatory network within the TERC locus (3q26.2). Conclusions: Overall, common regulatory variants in TERT did not show strong independent effects on EOAD susceptibility in PSEN1 A431E carriers. However, the convergence of association patterns, functional annotation, and regulatory evidence provides hypothesis-generating support for the TERC locus (3q26.2), particularly rs12696304, as a candidate region for further investigation. Additional studies integrating telomere dynamics, functional validation, and multi-omics analyses are needed to clarify the role of telomere biology in the pathogenesis of autosomal dominant EOAD. Full article

Soil adhesion and abrasive wear severely degrade the performance and service life of soil-covering rollers in no-tillage seeders, particularly in the heavy clay black soil regions of Northeast China. To address the critical issues of soil adhesion and wear on soil-covering rollers used in no-tillage seeders within black soil regions, this study presents a surface engineering strategy that integrates a bionic micro-texture with a functional composite coating. Inspired by the crescent-shaped pits on the body surface of Procambarus clarkii, a bionic texture was designed and combined with a PTFE/PDMS/TiO₂ composite coating. Key parameters were optimized using response surface methodology, yielding a TiO₂ mass fraction of 6%, coating thickness of 40 μm, remaining texture depth of 50 μm, and texture spacing of 250 μm. A prototype was fabricated and evaluated through orthogonal field experiments in two distinct soil environments. In clay soil (15–25% moisture content), soil moisture and vertical load significantly influenced anti-adhesion performance, with recommended operating parameters of 600 N vertical load and a speed range of 10.8–14.4 km·h⁻¹. In sandy soil (8–18% moisture content), vertical load and operating speed had significant effects on wear resistance, with optimal parameters identified as 600 N vertical load and 10.8 km·h⁻¹. Verification tests confirmed stable low-adhesion and low-wear performance under varying moisture conditions. Compared to conventional and PTFE-coated rollers, the bionic roller reduced soil adhesion by 82.62% and 74.02%, respectively, in high-moisture clay soil, and reduced wear loss by 36.81% and 28.97%, respectively, in dry sandy soil. These results demonstrate that the synergistic “structure–material” design, which leverages stress dispersion and storage from the bionic texture alongside low surface energy and enhanced wear resistance from the composite coating, offers a promising approach for improving the durability and performance of soil-engaging agricultural components. Full article

Systemic inflammation is influenced by regular physical activity and diet. While moderate exercise can transiently alter inflammatory markers, high-intensity activity may increase muscle turnover without substantially elevating systemic inflammation. The combined effects of physical activity and dietary inflammatory potential in healthy young men remain poorly defined. In this cross-sectional observational study, 233 healthy men aged 18–30 years were categorized according to physical activity level: low (NA, n = 52), moderate (A, n = 93), and high (S, n = 88). Anthropometry and body composition were assessed using bioelectrical impedance. Dietary intake was recorded over 4 days and used to calculate the Dietary Inflammatory Index (DII). Blood samples were collected and analyzed for complete blood counts, high-sensitivity C-reactive protein (hs-CRP), serum amyloid A (SAA), and creatine kinase (CK). Differences between groups were evaluated using the Kruskal–Wallis test with Dunn’s post hoc correction, and principal component analysis (PCA) was performed to explore multivariate inflammatory patterns. The highest BMI, fat percentage, and DII were observed in low-activity men, whereas fat-free mass and CK activity were greatest in highly active men. Slightly higher systemic inflammatory markers (hs-CRP and SAA) were observed in moderately active men compared to other groups. PCA revealed two principal axes: PC1 representing systemic inflammation and PC2 representing leukocyte distribution. Weak associations were found between DII and these components, indicating a limited link between dietary inflammatory potential and circulating inflammatory biomarkers. Body composition is strongly influenced by physical activity, with high activity promoting lean mass and moderate activity associated with modest elevations in inflammatory markers. Dietary inflammatory potential was only weakly associated with systemic inflammation, suggesting that exercise-induced physiological stress may play a more prominent role in shaping inflammatory profiles in healthy young men. Full article

Microcystin-LR (MC-LR) is a potent hepatotoxin that has been shown to cause liver damage even at doses lower than the established Low Observable Adverse Effect Level (LOAEL) of 200 μg/kg in animal models. We have previously observed that low-dose exposure to MC-LR in animals with diet-induced Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) and subsequent treatment with antioxidants like N-acetylcysteine (NAC) and the Na⁺/K⁺ ATPase-Src kinase inhibitor pNaKtide significantly alleviated hepatic infiltration of immune cells, downregulated markers of inflammation and hepatotoxicity, increased the breakdown of the toxin molecule, and restored phase I and II drug metabolism pathways, including the glutathione pathway. Because the liver is composed of heterogeneous cell types, this study aimed to determine the specific role of hepatocytes in the uptake and metabolism of MC-LR, especially in the setting of MASLD. To address this, we used two well-established hepatocyte cell lines—AML-12 murine hepatocytes and human Hep3B hepatocytes. Preliminary dose comparison studies with AML-12 cells showed that MC-LR at 10 μM concentration showed a significant upregulation in the genetic expression of the markers of hepatotoxicity—OSMR (p ≤ 0.01) and SerpinE (p ≤ 0.0001)—in comparison to Vehicle. Treatment with pNaKtide (1 µM) and/or NAC (10 mM) in the presence of MC-LR significantly reduced the expression of both OSMR (p ≤ 0.0001) and SerpinE (p ≤ 0.01 and p ≤ 0.0001, respectively). To model steatotic hepatocytes characteristic of the MASLD phenotype, Hep3B hepatocytes were first treated with 500 µM of oleic acid (OA) before exposing them to the toxin in the presence and absence of antioxidants. MC-LR exposure, induced markers of inflammation and hepatotoxicity to be elevated significantly in the presence of OA as compared to MC-LR exposure alone. This elevation of the genetic markers of inflammation and hepatotoxicity was significantly attenuated on treatment with pNaKtide (1 µM) and NAC (10 mM). Quantification of human SERPINE1 (PAI1) and 8-OHdG, a stable marker of oxidative stress, in the spent media of Hep3B cells corroborated the trends observed in the genetic markers of hepatotoxicity. These observations support the central role that hepatocytes play in the uptake and metabolism of MC-LR, which is complicated by the presence of MASLD-like conditions and can help in the development of future therapeutic strategies. Full article

Introduction: Distress during pediatric magnetic resonance imaging (MRI) neuroimaging can compromise scan quality and negatively impact children’s experiences. This review aimed to systematically synthesize biomarkers and psychological factors associated with distress in children, adolescents, and young adults undergoing neuroimaging. Methods: This systematic review was conducted according to PRISMA and AMSTAR-2 guidelines and preregistered in OSF. A systematic search was performed in six electronic databases, including observational articles published between 2000 and 2025 that assessed distress during MRI and functional MRI (fMRI). Data extraction and risk of bias assessment (QUIPS tool) were performed independently by two reviewers. Results: Ten studies (n = 558) examining distress during neuroimaging were included in this review. Distress was assessed through subjective self- and parent-reports, objective physiological measures, and qualitative interviews. Overall, distress levels were low to moderate; most participants tolerated scans well, though younger age, male sex, parental anxiety, procedure length, and chronic illness were associated with greater discomfort. Noise, immobility, and boredom emerged as the most frequent triggers, while strategies such as distraction, age-appropriate information, and reducing waiting times were perceived as helpful. Among participants with cancer, scan-related anxiety was closely linked to fear of recurrence and perceived stress. Risk of bias across studies was moderate to high, particularly in domains of attrition and statistical reporting. Conclusions: Distress during scanning is driven by anticipatory and parental anxiety, procedure length, and chronic illness. Biomarkers (e.g., cortisol, blood pressure) showed inconsistent links with subjective distress, highlighting the need for integrated measures. Full article

Firefighters are regularly exposed to occupational stress and potentially traumatic events. However, few evidence-based, fire service-specific interventions exist. Brief, mindfulness-based interventions may help address these challenges by improving regulation skills and reducing psychological distress. This pilot randomized controlled trial primarily evaluated the feasibility and acceptability of a one-session, group-based, virtual mindful attention training workshop developed specifically for firefighters, with secondary evaluation of preliminary efficacy. Firefighters (N = 82) were recruited from multiple fire departments across a large U.S. Southwestern metropolitan area and randomized to the mindful attention workshop (n = 45) or a waitlist control condition (n = 37). Feasibility outcomes were mixed, with strong enrollment among eligible participants (74.5%) but relatively low workshop attendance among those randomized to the intervention (53.3%). A total of 24 firefighters completed the workshop and found it to be helpful, informative, and relevant to the challenges faced in the fire service, with group processes characterized by high comfort, understanding, and low conflict. However, no significant between-group differences were observed in reductions in symptom severity or increases in mindfulness-based outcomes. Post hoc descriptive analyses revealed that most firefighters expressed strong interest in digitally delivered mental health content and the vast majority perceived online or app-based firefighter-specific mental health resources as helpful. Findings indicate mixed feasibility, strong acceptability among attendees, and a lack of preliminary efficacy, and highlight directions for refining intervention delivery of this pilot workshop and evaluating clinical impact in future trials. Full article

Current scientific reports on pain pharmacotherapy focus on the side effects of opioid medications related to dysregulation of the oxidative–antioxidant balance and immunomodulation. Initial observations concerned the use of opioids in the treatment of acute postoperative and cancer pain. Little is known about oxidative stress modulation in multi-modal opioid-based analgesia for chronic non-cancer pain. The aim of this study was to describe oxidative stress using plasma total antioxidant capacity (TAC) and total oxidative capacity (TOC), to assess whether these metrics are dependent on pain intensity and the scheme of analgesia. The study group consisted of patients with chronic low back pain, who were divided under the following treatments: multi-modal opioid-based therapy (n = 42), monotherapy with opioids (n = 28), and the control group (n = 11). A significantly lower TAC was observed in the study group compared to the monotherapy and control groups (220 µmol/L vs. 295 µmol/L, p = 0.02 vs. 399 µmol/L, p = 0.01). TOC was significantly lower in the polytherapy group compared to the monotherapy group (594 µmol/L vs. 723 µmol/L, p = 0.0002). A significantly lower TAC was observed in the typical analgesia scheme compared to the adjuvant analgesia model (260 µmol/L vs. 339 µmol/L, p = 0.01). The TAC in the severe pain classification was significantly lower than in the moderate group (p = 0.03). Multi-modal therapy with opioids significantly reduced oxidative activity compared to monotherapy but did not improve antioxidant capacity. Opioid-based pain therapy combined with adjuvant analgesics produced better antioxidant properties, and the antioxidant capacity was lower in severe pain scores. Full article

We evaluated the interplay between systemic total antioxidant capacity (TAC), oxidative stress (OS) (lipid hydroperoxides), inflammation, iron status, and oral contraception (OC) use in 182 healthy 23-year-old women (76 OC-users, and 106 non-OC-users). In all women, blood TAC (FORD units) values were significantly inversely associated with OS (FORT units), high-sensitivity C-reactive protein (hsCRP), and transferrin; and positively associated with transferrin saturation (TfS%). No significant associations were observed for hemoglobin, hematocrit, red blood cells, serum iron, soluble transferrin receptor (sTfR), sTfR/log(ferritin) ratio (sTfR-F index), ferritin, folate, uric acid, or creatinine. OS hydroperoxides were positively associated with hsCRP and transferrin, and inversely associated with TfS%. sTfR was positively correlated with hydroperoxides in non-OC-users and with folate in all women and non-OC-users, but was not associated with hsCRP in any group. The combined abnormal condition of low TAC and elevated OS (n = 71) was significantly more frequent among OC-users (OR = 39.0), women with hsCRP ≥ 3 mg L⁻¹ (OR = 10.1), transferrin ≥ 330 mg dL⁻¹ (OR = 6.58), and smokers (OR = 3.76). OC use modulated the TAC/OS balance and inflammation. Low TAC and elevated OS may impact health status. Enhanced TAC/OS knowledge may increase awareness of effects of OC use among fertile-age women. Ferritin was independent of TAC/OS status and OC use, supporting its reliability as an iron biomarker. Full article

Permeability is a critical parameter governing the gas flow behavior of the coalbed methane (CBM) reservoir during the exploration and exploitation of CBM, as well as the geological storage of CO₂ in the coalbeds. It is strongly associated with the multi-scale fractures developed in coal. Based on the distribution characteristics of micro-fractures, a multi-scale permeability model for coal reservoirs was established by introducing the permeability tensor, which comprehensively considers adsorption-induced coal swelling, pore pressure, effective stress, and micro-fractures. Further, the dynamic evolution law and mechanism of multi-scale permeability of coal reservoirs under different adsorption pressures were discussed. The results indicate that the increase in effective stress on the coal caused by adsorption-induced swelling essentially leads to a decrease in the equivalent multi-scale permeability of coal. Two key indicators, namely equilibrium pressure and rebound pressure, were defined to quantitatively characterize the evolution law of the equivalent multi-scale permeability during gas adsorption or desorption processes. The effective stress generated by the CO₂ adsorption-induced swelling effect in the low-rank coal is 1.47 times that in the middle-rank coal and 2.51 times that in the high-rank coal. Additionally, the effective stress generated by the CO₂ adsorption-induced swelling effect in the low-rank coal is 5.15 times that generated by N₂, while this level is 4.32 times higher than that in the middle-rank coal. Therefore, compared with the low- and middle-rank coal, the high-rank coal exhibits a smaller decrease in multi-scale permeability due to its weaker adsorption-induced swelling effect. During N₂ adsorption, the pore pressure effect dominates over the adsorption-induced swelling effect, resulting in a decrease in the effective stress on the coal with increasing gas pressure. Consequently, the equivalent multi-scale permeability of coal will increase much more significantly with an increase in injected N₂ pressure than with an increase in CO₂ pressure. By accounting for the differences between the effects of adsorption-induced swelling and pore compression on the equivalent multi-scale permeability of coal reservoir, the injectivity of CO₂ can be improved by mixing it with N₂. Full article

Given the established interplay between oxidative stress, cholinergic dysfunction, and metabolic imbalance in cognitive decline, this study investigated the multifunctional potential of three red macroalgae from the Madeira Archipelago (Asparagopsis taxiformis, Grateloupia lanceola, and Nemalion elminthoides) using a sequential biorefinery approach. Marine algae represent a sustainable source of functional food ingredients due to their rich content in bioactive compounds and their compatibility with low-impact production systems. Protein, ethanolic (phenolic-rich), and polysaccharide fractions were obtained through direct extraction and scalable biorefinery processing. Antioxidant activity was evaluated using ORAC, DPPH, FRAP, and FIC assays, while functionality relevant to human health was assessed through acetylcholinesterase, butyrylcholinesterase, and α-glucosidase inhibition. Protein extracts, particularly from N. elminthoides, exhibited strong hydrogen atom transfer-based antioxidant capacity, whereas ethanolic extracts demonstrated multifunctional activity, combining radical scavenging, metal chelation, and enzyme inhibition associated with neuroprotective and glycemic-regulation potential. Polysaccharide fractions contributed mainly to iron chelation and reducing capacity. Correlation analyses highlighted the complementary nature of antioxidant and bioactivity assays. Overall, these findings support the potential of Madeira red macroalgae as functional food ingredients and emphasize the importance of optimized biorefinery strategies to maximize nutritional and health-related benefits. Full article

Low glycemic-index foods may reduce postprandial oxidative stress by reducing postprandial glucose excursions, but the evidence for this is limited by dietary confounders. To determine whether reducing postprandial glucose per se reduces postprandial oxidative stress, overnight-fasted participants (BMI 25.0–39.9 kg/m², n = 18) consumed four test meals in random order: 75 g dextrose solution (Dex) within 5 min (bolus/noC), Dex slowly over 3.25 h (sipping/noC), bolus with 1 g vitamin C (bolus/C) and sipping with 1 g vitamin C (sipping/C). Venous blood was taken at intervals over 6 h; a standard lunch was consumed at 4 h. Sipping flattened postprandial glucose and insulin and reduced free fatty acid rebound compared to bolus (p < 0.05). Vitamin C raised serum vitamin C from ~20 to ~55 μmol/L. The total peroxyl radical trapping antioxidant potential (TRAP) increments differed after lunch, with a main effect of vitamin C at 5 h (mean ± SEM; C 70 ± 23 vs. noC −29 ± 27; p = 0.016) and main effects of rate (sipping 57 ± 25 vs. bolus −71 ± 28; p = 0.0002) and vitamin C (C 58 ± 25 vs. noC −73 ± 28; p = 0.0003) at 6 h. By multiple regression analysis, the TRAP area under the curve (AUC) was positively associated with the insulin AUC (p < 0.001) and negatively with the glucose and vitamin C AUCs (p < 0.05). The oxidized LDL increments were higher 6 h after sipping than bolus (7 ± 7 vs. −20 ± 7, p = 0.005). The oxidized LDL AUC was negatively associated with the TRAP AUC (p < 0.001). These results support the hypothesis that reducing postprandial glucose reduces postprandial oxidative stress. Full article

Background: The wear resistance of modern commercial glass-ceramic materials used in dental prostheses was investigated under cyclic contact conditions that included a rotational component. This loading mode has been largely overlooked in conventional in vitro wear testing, yet may be clinically relevant in patients with parafunctional conditions such as bruxism. Methods: Rotational loading was applied using an all-electric testing machine equipped with a biaxial actuator. Loading cycles combined a normal load (50 N) and a rotation (30°), at a frequency of 1 Hz. Microstructure and damage were characterized using advanced microscopy. Results: Rotational loading induced substantial damage across this class of materials, including the formation of glassy tribolayers with limited protective capability under the low-humidity conditions examined. Significant microstructure-dependent variations in wear volume were observed, with specific wear rates indicating severe wear (SWR above 10⁻⁶ mm³/N·m threshold) in three of the five materials tested. Lithium disilicate glass-ceramics, characterized by a high fraction of elongated reinforcement crystals, exhibited the greatest resistance to damage, whereas leucite-based glass-ceramics showed the lowest. The dominant wear mechanisms were plastic-deformation-induced grooving and fracture-driven chipping. The findings are interpreted within established wear models for brittle materials (Archard and fracture-based) and supported by numerical simulations of stress fields across multiple length scales. Implications: The results provide mechanistic insight into rotational wear damage in glass-ceramic systems, a material class particularly susceptible to such loading, and inform strategies for material selection and microstructural design aimed at improving prosthetic durability. Full article