Search Results (141)

Objectives: To explore the experiences of ex-smokers acting as peer supporters delivering chat-based instant messaging smoking cessation support in a post- randomized controlled trial qualitative study. Methods: We used purposive sampling to recruit 21 ex-smokers, who had previously provided chat-based instant messaging behavioral support to smokers in Hong Kong. Individual online semi-structured interviews were conducted. All interviews were audiotaped and transcribed verbatim. The transcripts were analyzed using inductive thematic analysis following Braun and Clarke’s six-phase framework. Results: Five themes were identified: stepping into the supporter role as ex-smokers, contributions beyond facilitating smoking cessation, mutual benefits as ex-smoking peer supporters, challenges in providing support, and the potential of mobile health-based peer support. Participants identified experiential sharing as a key mechanism for engaging motivated smokers, although its impact was limited by low user responsiveness. They described extending their support beyond smoking cessation (e.g., stress relief and healthier lifestyles), and reported mutual benefits, such as enhanced happiness, a positive attitude, and prevention of smoking relapse. Nevertheless, participants’ ability to effectively support smoking was challenged by limited interaction in the mHealth setting, difficulties offering timely responses, and uncertainty about how to handle complex personal situations. They suggested that future mobile health peer support can consider involving famous people as peer supporters, using personalized messaging, and adding incentives in the program to increase smokers’ motivation. Conclusions: Ex-smoking peer support in mHealth settings appears feasible and mutually beneficial, but its effectiveness may be constrained by low engagement and unclear role expectations. Full article

Alcohol-related liver disease (ALD) and ALD-related mortality are associated with hemolysis, increased erythrophagocytosis, and disturbed iron homeostasis. While macrophage-mediated erythrophagocytosis is well established, we investigated the contribution of liver sinusoidal endothelial cells (LSECs) to handling oxidatively damaged or ethanol-primed red blood cells (RBCs) in ALD. Live-cell imaging demonstrated that damaged RBCs were rapidly taken up by SK-HEP1 cells, an endothelial cell line with LSEC-like characteristics, and RBC uptake was associated with induction of heme oxygenase-1 (HO-1) and activation of its upstream regulator Nrf2. siRNA-mediated knockdown of the scavenger receptor Stabilin-1 attenuated RBC-induced HO-1 expression, supporting a role for Stabilin-1 in efferocytic signaling. Exposure of RBCs to ethanol concentrations as low as 25 mM induced phosphatidylserine externalization and rendered erythrocytes efferocytosis-competent. Lysed RBCs and free hemin elicited comparable oxidative stress responses. In murine models of hemolysis and chronic ethanol feeding, hemoglobin-derived signals were detected within sinusoidal structures showing a diffuse CD206-positive distribution pattern consistent with the sinusoidal scavenger compartment. Similar signals were observed in sinusoidal endothelial regions in human heavy drinkers with clinical signs of hemolysis. Together, these data suggest that LSECs may represent an additional component of RBC clearance in ALD, alongside macrophages and hepatocytes, with implications for hepatic iron handling. Full article

This paper proposes a low weight hybrid battery–supercapacitor energy storage system interfaced with bidirectional DC/DC converters with high power/current capability for aircraft applications. The supercapacitor converter having high power uses two pairs of interleaved coupled inductors to reduce the overall current ripple whilst increasing the converter’s power density. Due to the sensitive performance to saturation of the coupled inductors, a phase current balancing strategy is proposed to counter the effect current imbalance in the channels that would cause saturation degrading overall performance. A power management strategy (PMS) is implemented along with a low pass filter to separate the supercapacitor high frequency power component reference from the battery low frequency power component; therefore, separating the energy and power requirement for the energy storage system contributing to minimizing its weight whilst ensuring the current/power stresses are correctly handled. The validity of the system design is validated by a series of transient tests is conducted both in a simulation model as well as experimentally. Full article

Objective: This study aims to investigate the protective effect of Shengmai San (SMS) against high-glucose (HG)-induced injury in neonatal rat ventricular myocytes (NRVMs) and to elucidate the underlying pharmacological molecular mechanisms. We hypothesize that SMS ameliorates HG-induced calcium homeostasis imbalance in NRVMs by improving mitochondrial energy metabolism disorder, and this protective effect is associated with the downregulation of oxidized and phosphorylated CaMKII expression to inhibit CaMKII signaling pathway overactivation. Herein, we verify this hypothesis by assessing mitochondrial function, calcium transients, sarcoplasmic reticulum (SR) calcium handling and CaMKII phosphorylation levels in NRVMs. Methods: First, ultra-high performance liquid chromatography–high resolution mass spectrometry was used to identify the chemical components of SMS to clarify its material basis. Primary NRVMs were then cultured under low-glucose (LG) or HG conditions, with 2% SMS-medicated serum (SMS-MS) as the experimental intervention, and NAC (ROS scavenger) and KN93 (CaMKII inhibitor) as positive controls. Following intervention, we sequentially detected key indicators corresponding to the proposed pathological pathway: intracellular reactive oxygen species (ROS) levels (oxidative stress), mitochondrial ROS, mitochondrial function indices including oxygen consumption rate (OCR) (energy metabolism), calcium transients and diastolic intracellular free calcium concentration (global calcium homeostasis), sarcoplasmic reticulum (SR) calcium leak (calcium handling disorder), and, finally, the phosphorylation, oxidation levels of CaMKII and RyR2 phosphorylation (Ser2814) (p-RyR2) (key regulatory pathway) via Western blot to systematically elucidate the mechanistic link between SMS intervention and HG-induced NRVM injury. Results: Quantitative analysis revealed that high-glucose (HG) induction significantly reduced calcium transient amplitude and prolonged the decay time constant (tau) in NRVMs at 72 h (p < 0.01 vs. LG), with these parameters normalizing by 120 h—an effect indicative of a compensatory adaptive response. The 2%SMS-MS markedly ameliorated HG-induced calcium transient abnormalities at 72 h (p < 0.01 vs. HG). Additionally, 2%SMS-MS significantly enhanced mitochondrial basal oxygen consumption rate, spare respiratory capacity, ATP production, and maximal respiration in HG-exposed NRVMs (p < 0.01 vs. HG). SMS also significantly reduced intracellular reactive oxygen species (ROS) levels (p < 0.01 vs. HG), mitochondrial ROS levels (p < 0.01 vs. HG), diastolic intracellular free calcium concentration (p < 0.01 vs. HG), and SR calcium leak (p < 0.05 vs. HG). Western blot analysis revealed that 2%SMS-MS intervention effectively downregulated the expression of oxidized CaMKII (Ox-CaMKII) (p < 0.01 vs. HG), phosphorylated CaMKII (p-CaMKII) (p < 0.01 vs. HG), and RyR2 phosphorylation (Ser2814) (p < 0.05 vs. HG), which may be the potential mechanism in maintaining calcium homeostasis in HG-induced NRVMs. Conclusions: This study suggests that SMS enhances mitochondrial energy metabolism and exerts a protective effect against high-glucose-induced calcium homeostasis imbalance in NRVMs, which supports our proposed hypothesis. Its potential mechanism indicates that the protective effects of SMS are associated with its ability to downregulate the expression of oxidized and phosphorylated CaMKII. These findings highlight SMS as a potential therapeutic candidate for alleviating HG-related myocardial injury and provide evidence for its application in the prevention of early diabetic cardiomyopathy. Full article

The load characteristics of the helicopter tail rotor system are critical to flight safety and handling performance, and flight testing remains the most direct and reliable means to obtain authentic load data. In this paper, the well-established Wheatstone bridge strain measurement method is adopted to carry out accurate load testing on the helicopter tail rotor system. The tail rotor assembly mainly consists of the tail rotor shaft, pitch link, and tail rotor blades, which undertake different load transfer tasks during flight. Under actual operating conditions, the tail rotor shaft bears significant axial tension as well as combined lateral and vertical bending moments; the pitch link is primarily subjected to alternating axial tension and compression; and the tail rotor blades withstand complex loads including flapping bending, lagwise bending, and torsional moments. According to the distinct stress characteristics and force transmission paths of each component, targeted flight test maneuvers are reasonably designed. These maneuvers include steady-level flight at low, medium, and high speeds, zigzag climbing flight, near-ground side-rear flight, as well as deceleration-to-sprint and obstacle slope maneuvers specified in ADS-33E. Key flight parameters are selected for in-depth analysis to reveal the load distribution and dynamic variation patterns of the tail rotor under typical operating conditions. On this basis, a helicopter load risk test point matrix is established to identify high-risk working conditions and key monitoring positions. This study provides a solid theoretical and data foundation for subsequent flight test monitoring and structural strength verification. It effectively reduces flight test risks, improves monitoring efficiency and accuracy, and helps cut down the human, material, and financial costs associated with flight test monitoring. The research results can also provide important references for the design optimization and safety evaluation of helicopter tail rotor systems. Full article

Heterogeneous multi-core systems (HMCSs) typically face a dilemma: heuristics (e.g., Linux CFS) are fast but blind to global constraints, while meta-heuristics (e.g., GAs) are globally optimal but too slow for real-time OS interaction. To bridge this gap without relying on “black-box” neural networks, we introduce the Tissue P System-Inspired Task Allocator (TPSTA). By mapping HMCS and parallel task scheduling to Tissue P System models and vectorized linear algebra problems, TPSTA achieves a computational complexity of $\mathrm{O}\left(\mathrm{M}/\mathrm{W}\right)$, effectively compressing the decision space. Our rigorous evaluation across four dimensions reveals a system strictly bound by physical constraints rather than algorithmic heuristics. (1) Under sufficient resource provisioning (four chips), TPSTA achieves a 0.00% Deadline Miss Ratio (DMR). Crucially, stress tests on constrained hardware (two chips) show graceful degradation to a 12.88% DMR, matching the optimal theoretical bound of EDF, whereas standard heuristics collapse to failure rates > 68%. On a massive 4096-core cluster, TPSTA outperforms the Linux GTS scalar baseline by 14.4×, maintaining low latency where traditional algorithms fail (>8 s). (3) Adaptability: The system demonstrates adaptive routing in handling hardware heterogeneity; without explicit rule-coding, it autonomously prioritizes data locality during NUMA transfers and migrates compute-bound tasks during thermal throttling events. (4) Physical Limits: Finally, our roofline analysis confirms that while the algorithmic speedup is theoretically linear, practical performance saturates at ~375× due to the Memory Wall, validating the isomorphism between synaptic bandwidth and hardware memory channels. Full article

Cuproptosis is a copper-dependent form of regulated cell death that is triggered when intracellular copper handling is perturbed and mitochondrial metabolism becomes the primary site of damage. Aging provides a biological context for this process because copper trafficking shifts, mitochondrial quality control and proteostasis decline, and immune function is remodeled toward immunosenescence with persistent low-grade inflammation. These age-associated changes can weaken antioxidant buffering, reshape labile copper pools, and lower the threshold at which copper stress is converted into mitochondrial proteotoxic injury. In parallel, inflammaging-related cytokines and NF-κB programs can alter copper import, export, and sequestration, while impaired efferocytosis prolongs danger signaling, creating feedforward loops that sustain tissue injury. In this review, we summarize the molecular features that distinguish cuproptosis from other death programs and discuss how redox buffering capacity, copper transport machinery, and mitochondrial metabolic state jointly determine cuproptosis sensitivity during aging. We then examine disease contexts in which these pathways are plausibly relevant, including hereditary copper-handling disorders and age-related neurodegenerative, cardiovascular, metabolic, and musculoskeletal disorders. Finally, we discuss key knowledge gaps and experimental priorities for interpreting cuproptosis-related signals in aged tissues, with emphasis on how copper handling, mitochondrial state, and immune remodeling jointly shape disease phenotypes. Full article

Dynamic characterization of calcareous (carbonate) sands is essential for performance-based design of offshore foundations, coastal reclamation, and marine infrastructure in tropical and subtropical regions. In contrast to silica sands, carbonate sediments are biogenic and typically comprise angular, irregular grains with intra-particle voids and fragile skeletal microstructure. These traits promote grain crushing and fabric evolution at relatively low-to-moderate confinement, leading to pronounced stress dependency, strong nonlinearity with strain amplitude, and substantial scatter in laboratory stiffness and damping measurements. Consequently, empirical correlations calibrated primarily on quartz sands may yield biased estimates when transferred to carbonate environments. This study presents an ML-driven, leakage-aware benchmarking framework for predicting two key dynamic parameters of biogenic calcareous sands, damping ratio $D$ and shear modulus $G$, using standard tabular descriptors commonly available in geotechnical practice. Two consolidated experimental databases were curated from resonant column and cyclic triaxial measurements ($D$: $n=890$; $G$: $n=966$), spanning mean effective confining stress $25 \le {\sigma }_m^{\prime }\le 1600 \mathrm{k}$Pa and a wide range of density and gradation conditions. To emphasize transferability, explicit deposit/site labels were excluded, and missingness arising from heterogeneous reporting was handled through a consistent preprocessing pipeline (training-only imputation, categorical encoding, and scaling). Eleven regression algorithms were evaluated, covering linear baselines, regularized regression, neighborhood learning, single trees, bagging and boosting ensembles, kernel regression, and a feedforward neural network. Performance was assessed using $R^2$, RMSE, and MAE on training/validation/test splits, and engineering credibility was supported through explainability-based diagnostics to verify mechanically plausible sensitivities. Results show that ensemble-tree models (Extra Trees and Random Forest) provide the most reliable accuracy–robustness balance across both targets, consistently outperforming linear models and the tested SVR configuration and exhibiting stable validation-to-test behavior. The explainability audit confirms physically meaningful separation of governing controls: stiffness is primarily stress-controlled (${\sigma }_m^{\prime }$ dominant for $G$), whereas damping is primarily strain-controlled ($\gamma$ dominant for $D$). The proposed framework supports practical deployment as a fast surrogate for generating $G\hspace{0.17em}-\hspace{0.17em}\gamma$ and $D\hspace{0.17em}-\hspace{0.17em}\gamma$ curves within the training domain and for guiding targeted laboratory test planning in carbonate settings. Full article

The transition toward active distribution networks requires advanced control solutions capable of handling the rapid dynamics of distributed energy resources. This paper proposes a low-cost, intelligent IoT architecture designed for the real-time optimization and analysis of energy systems within low-voltage networks. Unlike centralized monitoring approaches constrained by communication latency, the proposed solution leverages Intelligent Edge Processing (IEP) implemented on ESP32 embedded nodes to optimize data flow and decision-making. This architecture executes stability assessments directly at the network edge, calculating critical analysis indicators such as the Voltage Deviation Index (VDI) and Rate of Change of Frequency (RoCoF). The system was validated on the CIGRE European LV benchmark under severe stress scenarios, including rapid solar transients and voltage sags. The results demonstrate that the proposed architecture effectively coordinates storage interventions, ensuring voltage recovery within 300 ms and maintaining power quality within EN 50160 limits even during severe voltage sags. The study validates the feasibility of using industrial IoT edge computing as a resilient, non-wire alternative for modernizing complex energy systems. Full article

Through room-temperature tensile testing, microstructural observation, and comparative analysis of dislocation configurations, this study investigates the deformation and damage behavior of a high-concentration Re/Ru single-crystal alloy. The results show that the alloy possesses excellent mechanical properties at room temperature, with a tensile strength of 875 MPa and a yield strength of 847 MPa. During tensile deformation, plastic strain primarily occurs through dislocation slip within the γ matrix and dislocation shear into the γ′ phase. Dislocations sheared into the γ′ phase exhibit distinct decomposition patterns. Microcracks initiate at γ′/γ interfaces where two slip systems intersect. As tensile loading continues, these microcracks coalesce, leading to increased local stress and unstable crack propagation along the γ/γ′ interfaces, ultimately resulting in fracture. This process constitutes the deformation and damage mechanism of the alloy during room-temperature tensile deformation. These findings suggest that high Re/Ru concentrations fundamentally alter low-temperature deformation pathways, which may improve resistance to brittle fracture during cold start or handling conditions. Full article

Vitamin D, a fat-soluble secosteroid traditionally recognized for skeletal health, exerts pleiotropic effects on cardiovascular physiology and disease. Circulating 25-hydroxyvitamin D [25(OH)D], the principal biomarker of vitamin D status, is frequently suboptimal worldwide, particularly in older adults, individuals with darker skin pigmentation, and populations at higher latitudes. Observational studies consistently associate low 25(OH)D concentrations with increased risk of hypertension, atherosclerosis, myocardial infarction, heart failure, arrhythmias, stroke, and cardiovascular mortality. Mechanistic investigations have revealed that vitamin D modulates cardiomyocyte calcium handling, endothelial function, vascular smooth muscle proliferation, inflammation, oxidative stress, and renin–angiotensin–aldosterone system activity, establishing biologically plausible links to cardiovascular outcomes. Despite these associations, large randomized trials of vitamin D supplementation have failed to demonstrate reductions in major cardiovascular events, likely due to heterogeneity in baseline status, dosing regimens, intervention timing, genetic variability, and underlying comorbidities. Vitamin D may function more effectively as a biomarker of cardiovascular risk rather than a universal therapeutic agent, with deficiency reflecting systemic vulnerability rather than acting as a dominant causal factor. Emerging evidence supports precision approaches targeting individuals with severe deficiency, high renin activity, early endothelial dysfunction, or specific genetic profiles, potentially in combination with lifestyle or pharmacologic interventions. Future research should focus on defining optimal dosing strategies, intervention timing, and mechanistic biomarkers to identify subpopulations most likely to benefit, integrating vitamin D therapy into multifaceted cardiovascular prevention frameworks. This systematic review synthesizes molecular, observational, and clinical trial evidence, critically evaluating the current understanding of vitamin D in cardiovascular medicine and highlighting opportunities for targeted, personalized interventions. Vitamin D represents a complex, context-dependent modulator of cardiovascular health, offering both prognostic insight and potential therapeutic value when appropriately applied. Full article

Nail clipping can cause fear, anxiety, and stress (FAS) in dogs and cats and possibly long-term aversion to veterinary care. We conducted an anonymous, online, mixed-methods survey to characterise the knowledge, attitudes, and practices of Australian veterinary nurses, technicians, and other veterinary staff regarding nail clipping in dogs and cats. Among 242 valid responses, 71.9% (n = 174/242) performed nail clipping multiple times per week. Dogs (72.4%, n = 165/228) and cats (59%, n = 134/227) frequently displayed FAS (score > 2/5) during nail clipping. Most respondents (79.8%, n = 193/242) reported being injured while performing nail clipping. Respondents reporting a negative attitude towards nail clipping were 5.5 times (95% CI = 1.7–17.8) more likely to report being injured during a nail clip compared to those with a positive attitude. Respondents reported feeling pressure to persevere with nail clipping when it was unnecessary or when animals exhibited severe FAS. Where used, pre-visit pharmaceuticals and sedation were reported to effectively reduce FAS (dog: p = 0.015; cat: p = 0.152), along with non-pharmaceutical interventions such as gentle handling and counter-conditioning. Respondents perceived nail-clipping to be a difficult, undervalued task. Increased training of veterinary team members regarding nail clipping could improve animal welfare and the safety of veterinary team members. Full article

Small-diameter synthetic grafts often fail from thrombosis, intimal hyperplasia, and compliance mismatch, highlighting the need for alternatives that better support endothelialization and remodeling. Here, we evaluated multilayer plant-based vascular grafts fabricated from decellularized leatherleaf viburnum reinforced with cross-linked gelatin, seeded with vascular smooth muscle cells and endothelial cells, and conditioned in a perfusion bioreactor to mimic physiological shear stress. Pre-implant assays confirmed effective decellularization, low residual detergent, and mechanical integrity suitable for surgical handling. In a rat abdominal aorta interposition model, plant-based grafts remained patent at 1, 4, and 24 weeks and showed higher survival than silicone controls. Ultrasound imaging demonstrated flow patterns and resistance indices similar to native vessels, and plant-based grafts maintained significantly higher endothelial cell coverage than silicone controls, reaching native-like density by 24 weeks. Histology and biochemical assays showed early collagen and elastin coverage comparable to native aorta and increased collagen by 24 weeks. Scanning electron microscopy showed smooth luminal surfaces with minimal thrombus formation, contrasting with the rougher, thrombus-prone surfaces of silicone grafts. These findings indicate that plant-based grafts support endothelialization, maintain long-term patency, and undergo favorable remodeling in vivo, supporting their potential as a biomimetic alternative for small-diameter arterial repair. Full article

Type 2 diabetes mellitus (T2DM) involves chronic hyperglycemia, insulin resistance, low-grade inflammation, and oxidative stress that drive cardiometabolic and renal damage despite current therapies. Sodium–glucose cotransporter (SGLT) inhibitors have reshaped the treatment landscape, but residual risk and safety concerns highlight the need for new agents that combine glucose-lowering efficacy with redox–inflammatory modulation. LASSBio-1986 is a synthetic N-acylhydrazone (NAH) derivative designed as a gliflozin-like scaffold with the potential to interact with SGLT1/2 while also influencing oxidative and inflammatory pathways. Here, we integrated in silico and in vivo approaches to characterize LASSBio-1986 as a multifunctional antidiabetic lead in murine models of glucose dysregulation. PASS and target class prediction suggested a broad activity spectrum and highlighted transporter- and stress-related pathways. Molecular docking indicated high-affinity binding to both SGLT1 and SGLT2, with a modest energetic preference for SGLT2, and ADME/Tox predictions supported favorable oral drug-likeness. In vivo, intraperitoneal LASSBio-1986 improved oral glucose tolerance and reduced glycemic excursions in an acute glucose challenge model in C57BL/6 mice, while enhancing hepatic and skeletal muscle glycogen stores. In a dexamethasone-induced insulin-resistance model, LASSBio-1986 improved insulin sensitivity, favorably modulated serum lipids, attenuated thiobarbituric acid-reactive substances (TBARS), restored reduced glutathione (GSH) levels, and rebalanced pro- and anti-inflammatory cytokines in metabolic tissues, with efficacy broadly comparable to dapagliflozin. These convergent findings support LASSBio-1986 as a preclinical, multimodal lead that targets SGLT-dependent glucose handling while mitigating oxidative and inflammatory stress in models relevant to T2DM. Chronic disease models, formal toxicology, and pharmacokinetic studies, particularly with oral dosing, will be essential to define its translational potential. Full article

There is a significant demand for flexibility in steam turbines, including rapid cold starts and load changes, as well as operation at low partial loads. Both industrial plants and systems for electricity and heat generation are impacted. These new operating modes result in complex, asymmetric temperature fields and additional thermally induced stresses. These lead to casing deformations, which affect blade tip gap and casing flange sealing integrity. The exact progression of heat flux and heat transfer coefficients within the cavities of steam turbines remains unclear. The current methods used in the calculation departments rely on simplified, averaged estimates, despite the presence of complex flow phenomena. These include swirling inflows, temperature gradients, impinging jets, unsteady turbulence, and vortex formation. This paper presents a novel sensor and its thermal measurements taken on a full-scale steam turbine test rig. Numerical calculations were performed concurrently. The results were validated by measurements. Additionally, the distribution of the heat transfer coefficient along the cavity was analysed. The rule of L’Hôpital was applied at specific locations. A method for handling axial variation in the heat transfer coefficient is also proposed. Measurements were taken under real-life conditions with a full-scale test rig at MAN Energy Solutions SE, Oberhausen, with steam parameters of 400 °C and 30 bar. The results at various operating points are presented. Full article