Search Results (514)

Urban wastewater is composed of nutrients such as nitrogen and phosphorus, organic matter, heavy metals, pathogens, and micropollutants. If untreated, these contribute to eutrophication and environmental degradation. Microalgae-based bioremediation offers a sustainable solution, showing promise for pollutant removal and high-value bioproduct generation. This study evaluates the efficacy of Galdieria sulphuraria ACUF 427 in treating urban wastewater, with a focus on nutrient removal and phycocyanin production at different optical densities (OD 2, OD 4, and OD 6). Nutrient removal rates (RRs) were analysed for ammonium nitrogen (N-NH₄⁺), ammonia nitrogen (N-NH₃), phosphate phosphorus (P-PO₄³⁻), and chemical oxygen demand (COD). The RR for N-NH₄⁺ increased with optical density, reaching 7.49 mg/L/d at an optical density of 6. Similar trends were observed for N-NH₃ and P-PO₄³⁻, with peak removal at OD 6. COD removal remained high across all ODs, though differences between OD 4 and OD 6 were not statistically significant. Significant variations (p < 0.05) in nutrient removal were noted across the ODs, except for COD between OD 4 and OD 6. Biomass growth and phycocyanin production were significantly higher in the wastewater compared to the control (Allen Medium), with the most effective performance observed at an optical density (OD) of 6. Maximum growth rates were 0.241 g/L/d at OD 6, 0.178 g/L/d at OD 4, and 0.120 g/L/d at OD 2. These results highlight the potential of G. sulphuraria as an agent for wastewater bioremediation and the production of high-value compounds, particularly at elevated cell densities, where we achieved superior nutrient removal and biomass production. Full article

Polymer inclusion membranes (PIMs) have undergone substantial advancements in their selectivity and efficiency, driven by their increasing deployment in separation processes, environmental remediation, and sensing applications. This review presents recent progress in the development of PIMs, focusing on strategies to enhance ion and molecule selectivity through the incorporation of novel carriers, including ionic liquids and task-specific extractants, as well as through polymer functionalization techniques. Improvements in mechanical and chemical stability, achieved via the utilization of high-performance polymers such as polyvinylidene fluoride (PVDF) and polyether ether ketone (PEEK), as well as cross-linking approaches, are critically analyzed. The expanded application of PIMs in the removal of heavy metals, organic micropollutants, and gas separation, particularly for carbon dioxide capture, is discussed with an emphasis on efficiency and operational robustness. The integration of PIMs with electrochemical and optical transduction platforms for sensor development is also reviewed, highlighting enhancements in sensitivity, selectivity, and response time. Furthermore, emerging trends towards the fabrication of sustainable PIMs using biodegradable polymers and green solvents are evaluated. Advances in scalable manufacturing techniques, including phase inversion and electrospinning, are addressed, outlining pathways for the industrial translation of PIM technologies. The review concludes by identifying current limitations and proposing future research directions necessary to fully exploit the potential of PIMs in industrial and environmental sectors. Full article

The presence of ibuprofen (IBP) and paracetamol (PAR) contaminants in wastewater has become an emerging issue. Traditional wastewater treatment facilities have not been adequately upgraded to remove these micropollutants. This study focused on screening and identifying effective rhizobacteria capable of assisting plants in eliminating ibuprofen and paracetamol from wastewater using constructed wetlands. A total of 28 rhizobacteria were isolated from both the roots and the surrounding sand of Scirpus grossus after 30 days of pharmaceutical exposure. Among these, three isolates (Gram-negative Enterobacter aerogenes, Gram-positive Bacillus flexus, and Paenibacillus alvei) showed high tolerance to IBP and PAR with initial removal efficiencies > 75%. The addition of these three isolated rhizobacteria to a constructed wetland (planted with Scirpus grossus, 5-day HRT, 2 L/min aeration) assists the removal of IBP and PAR from wastewater. Bioaugmentation of rhizobacteria showed an increment of IBP removal (↑13%) from water (residual of 10 µg/L) and PAR (↑20%) from sand (residual 2.3 µg/L) as compared to the non-bioaugmented systems. The addition of rhizobacteria also showed the ability to significantly enhance the translocation of PAR into the shoot system of S. grossus, suggesting assisted phytoextraction mechanisms, while the removal of IBP in wetlands is suggested to occur via rhizodegradation. It is recommended that future research be conducted to elucidate the microbial degradation pathways and analyze the intermediate metabolites to accurately depict the pharmaceutical degradation mechanisms and evaluate their ecological risks. Full article

This study investigates the adsorption behavior of 4-methylbenzylidene camphor (4-MBC), a persistent ultraviolet filter, onto microplastic fibers (MPFs) released from domestic textiles, under environmentally relevant conditions. Two types of MPFs were used: MPF A, a heterogeneous blend of synthetic and natural fibers, and MPF B, a uniform polyester source. Adsorption experiments were conducted in municipal wastewater, Danube River surface water, and laundry effluent. Kinetic data best fit the pseudo-second-order model (R² > 0.95), and the Elovich model indicated chemisorption involving heterogeneous binding sites. MPF A exhibited superior adsorption capacities (qₑ = 85.4–90.1 µg/g) compared to MPF B (58.8–66.8 µg/g). Langmuir isotherms yielded maximum adsorption capacities of 204.9 µg/g for MPF A and 116.7 µg/g for MPF B (R² = 0.929–0.977), while D–R isotherm energies (12.0–21.7 kJ/mol) confirmed specific interactions, such as π–π stacking and hydrogen bonding. Adsorption efficiency was highest in municipal wastewater (total organic carbon—TOC = 13.12 mg/L, electrical conductivity—EC = 1152 µS/cm), followed by laundry and surface waters. These findings emphasize the critical role of polymer composition and matrix complexity in pollutant transport, suggesting MPFs are effective transporters of hydrophobic micropollutants in aquatic systems. Full article

Herein, we rationally designed a molecular catalytic filter for effective micropollutants decontamination via peroxymonosulfate (PMS) activation. Specifically, iron phthalocanine (FePc) molecules with defined Fe–N₄ coordination were immobilized onto carbon nanotubes (CNTs), forming a hybrid catalyst that integrated molecular precision with heterogeneous catalytic properties. The resulting CNT-FePc filter achieved a 98.4% removal efficiency for bisphenol A (10 ppm) in a single-pass operation system, significantly outperforming the CNT/PMS system without FePc (41.6%). Additionally, the CNT-FePc/PMS system demonstrated remarkable resistance to performance inhibition by common water matrix components. Unlike typical radical-dominated PMS activation processes, mechanistic investigations confirmed that the CNT-FePc/PMS system selectively promoted singlet oxygen (¹O₂) generation as the primary oxidative pathway. Density functional theory (DFT) calculations revealed that PMS exhibited stronger adsorption on FePc (−3.05 eV) compared to CNT (−2.86 eV), and that FePc effectively facilitated O–O bond elongation in PMS, thereby facilitating ¹O₂ generation. Additionally, seed germination assays indicated a significant reduction in the biotoxicity of the treated effluents. Overall, this work presents a catalyst design strategy that merges molecular-level coordination chemistry with practical flow-through configuration, enabling rapid, selective, and environmentally benign micropollutant removal. Full article

The increasing use of pharmaceutically active compounds (PhACs), endocrine-disrupting compounds (EDCs), and personal care products (PCPs) has led to the widespread presence of organic micropollutants (OMPs) in aquatic environments, posing a significant global challenge for environmental conservation. In recent years, advanced materials-based nanofiltration (NF) technologies have emerged as a promising solution for water and wastewater treatment. This review begins by examining the sources of OMPs, as well as the risk of OMPs. Subsequently, the key criteria of NF membranes for OMPs are discussed, with a focus on the roles of pore size, charge property, molecular interaction, and hydrophilicity in the separation performance. Against that background, this review summarizes and analyzes recent advancements in materials such as metal organic frameworks (MOFs), covalent organic frameworks (COFs), graphene oxide (GO), MXenes, hybrid materials, and environmentally friendly materials. It highlights the porous nature and structural diversity of organic framework materials, the advantage of inorganic layered materials in forming controllable nanochannels through stacking, the synergistic effects of hybrid materials, and the importance of green materials. Finally, the challenges related to the performance optimization, scalable fabrication, environmental sustainability, and complex separation of advanced materials-based membranes for OMP removal are discussed, along with future research directions and potential breakthroughs. Full article

Fungicides are compounds with potentially toxic effects on the human body, but the molecular mechanisms of their action have not yet been explained. The effect of cyprodinil on cell viability, apoptosis level, cell membrane function, cell morphology and expression of antioxidant enzyme genes in the A-375 and DLD-1 cell lines was examined. The cell lines were selected because they can be an excellent in vitro model of neoplastic changes occurring in the skin and large intestine after exposure to a fungicide. The fungicide selected for the study is commonly used in Poland to protect crops against fungi. Our results showed that the tested compound increased cell viability and proliferation, probably activated by mechanisms related to oxidative stress. Cyprodinil caused an increase in glutathione level (in A-375 by about 37% and in DLD-1 by about 28%) and oxidative stress enzymes activity, but not in apoptosis level. Its membrane interactions and its penetration into cells was concentration dependent. It is worth emphasizing that the novelty of our work lies in the use of non-traditional toxicological methods based on molecular analyses using human cell lines. This allowed us to demonstrate not only the toxicity of a single substance but also its behavior within cellular structures. Our findings suggest that cyprodinil may have tumor-promoting properties in skin and colorectal cancer cells. Full article

Concern over the presence of pharmaceutical waste in the environment has prompted research into the management of emerging organic micropollutants (EOMs). In response, sustainable technologies have been applied as alternatives to reduce the effects of these contaminants. This study investigated the capacity of filamentous fungi isolated from iron mine soil in the Amazon region to biodegrade the drug chloroquine diphosphate. An initial screening assessed the growth of four fungal strains on solid media containing chloroquine diphosphate: Trichoderma pseudoasperelloides CBMAI 2752, Penicillium rolfsii CBMAI 2753, Talaromyces verruculosus CBMAI 2754, and Penicillium sp. cf. guaibinense CBMAI 2758. Among them, Penicillium sp. cf. guaibinense CBMAI 2758 was selected for further testing in liquid media. A Box–Behnken factorial design was applied with three variables, pH (5, 7, and 9), incubation time (5, 10, and 15 days), and chloroquine diphosphate concentration (50, 75, and 100 mg·L⁻¹), totaling 15 experiments. The samples were analyzed by gas chromatography–mass spectrometry (GC-MS). The most effective conditions for chloroquine biodegradation were pH 7, 100 mg·L⁻¹ concentration, and 10 days of incubation. Four metabolites were identified: one resulting from N-deethylation M1 (N4-(7-chloroquinolin-4-yl)-N1-ethylpentane-1,4-diamine), two from carbon–carbon bond cleavage M2 (7-chloro-N-ethylquinolin-4-amine) and M3 (N1,N1-diethylpentane-1,4-diamine), and one from aromatic deamination M4 (N1-ethylbutane-1,4-diamine) by enzymatic reactions. The toxicity analysis showed that the products obtained from the biodegradation of chloroquine were less toxic than the commercial formulation of this compound. These findings highlight the biotechnological potential of Amazonian fungi for drug biodegradation and decontamination. Full article

In response to the European Union’s revised Urban Wastewater Treatment Directive, which mandates enhanced monitoring and advanced treatment of micropollutants, this study was conducted. It took place within the Coastal Landscape Park (CLP), a Natura 2000 protected area in northern Poland. The focus was on the municipal wastewater treatment plant in Jastrzębia Góra, located in a region exposed to seasonal tourist pressure and discharging effluent into the Czarna Wda River. A total of 90 wastewater samples were collected during five monitoring campaigns (July, September 2021; February, May, July 2022) and analysed for 13 pharmaceuticals and personal care products (PPCPs) using ultra-high-performance liquid chromatography tandem mass spectrometry with electrospray ionisation (UHPLC-ESI-MS/MS). The monitoring included both untreated (UTWW) and treated wastewater (TWW) to assess the PPCP removal efficiency and persistence. The highest concentrations in the treated wastewater were observed for metoprolol (up to 472.9 ng/L), diclofenac (up to 3030 ng/L), trimethoprim (up to 603.6 ng/L) and carbamazepine (up to 2221 ng/L). A risk quotient (RQ) analysis identified diclofenac and LI-CBZ as priority substances for monitoring. Multivariate analyses (PCA, HCA) revealed co-occurrence patterns and seasonal trends. The results underline the need for advanced treatment solutions and targeted monitoring, especially in sensitive coastal catchments with variable micropollutant presence. Full article

Untreated sewage sludge (SS) and misused stabilization technologies have contributed to great contamination and the accumulation of various pollutants in agricultural soils. Regarding micro-pollutants’ degradation, scalable and effective technologies are still scarce. Although many attempts at composting adaptations have been discussed, only a few have been tested individually under outdoor conditions. To investigate different composting methods (bioaugmentation and semipermeable cover) for the removal of micro-pollutants frequently found in SS, we performed a set of on-site experiments. Windrows of SS and olive pruning were used as the compostable material and were subjected to (i) bioaugmentation with the fungus Penicillium oxalicum, (ii) covered composting, (iii) covered and bioaugmented composting, and (iv) a conventional composting pile, which was included as a control. The entire experiment lasted 99 days. Bioaugmentation without cover increased the phosphorus content, favored a reduction in heavy metal content, and was the only treatment that reduced carbamazepine at the end of the process. Moreover, the inoculation of P. oxalicum under semipermeable cover increased the richness, diversity, and dominance of specific microbial taxa and total bacterial abundance. The four mature composts obtained met the standards required to be classified in the B fertilizer category, showing that we reduced most of the micro-pollutants, and passed the germination test. Full article

The widespread and persistent occurrence of micropollutants—such as pesticides, pharmaceuticals, heavy metals, personal care products, microplastics, and per- and polyfluoroalkyl substances (PFAS)—has emerged as a critical environmental and public health concern, necessitating the development of highly sensitive, selective, and field-deployable detection technologies. Microfluidic sensors, including biosensors, have gained prominence as versatile and transformative tools for real-time environmental monitoring, enabling precise and rapid detection of trace-level contaminants in complex environmental matrices. Their miniaturized design, low reagent consumption, and compatibility with portable and smartphone-assisted platforms make them particularly suited for on-site applications. Recent breakthroughs in nanomaterials, synthetic recognition elements (e.g., aptamers and molecularly imprinted polymers), and enzyme-free detection strategies have significantly enhanced the performance of these biosensors in terms of sensitivity, specificity, and multiplexing capabilities. Moreover, the integration of artificial intelligence (AI) and machine learning algorithms into microfluidic platforms has opened new frontiers in data analysis, enabling automated signal processing, anomaly detection, and adaptive calibration for improved diagnostic accuracy and reliability. This review presents a comprehensive overview of cutting-edge microfluidic sensor technologies for micropollutant detection, emphasizing fabrication strategies, sensing mechanisms, and their application across diverse pollutant categories. We also address current challenges, such as device robustness, scalability, and potential signal interference, while highlighting emerging solutions including biodegradable substrates, modular integration, and AI-driven interpretive frameworks. Collectively, these innovations underscore the potential of microfluidic sensors to redefine environmental diagnostics and advance sustainable pollution monitoring and management strategies. Full article

Carbonaceous sorbents were prepared from Chlorella vulgaris via hydrothermal carbonization (200 °C and 250 °C) and slow pyrolysis (300–500 °C) to assess their effectiveness in removing the herbicide metribuzin from water. The biomass was cultivated under controlled laboratory conditions, allowing for consistent feedstock quality and traceability throughout processing. Using a single microalgal feedstock for both thermal methods enabled a direct comparison of hydrochar and pyrochar properties and performance, eliminating variability associated with different feedstocks and allowing for a clearer assessment of the influence of thermal conversion pathways. While previous studies have examined algae-derived biochars for heavy metal adsorption, comprehensive comparisons targeting organic micropollutants, such as metribuzin, remain scarce. Moreover, few works have combined kinetic and isotherm modeling to evaluate the underlying adsorption mechanisms of both hydrochars and pyrochars produced from the same algal biomass. Therefore, the materials investigated in the present work were characterized using a combination of standard physicochemical and structural techniques (FTIR, SEM, BET, pH, ash content, and TOC). The kinetics of sorption were also studied. The results show better agreement with the pseudo-second-order model, consistent with chemisorption, except for the hydrochar produced at 250 °C, where physisorption provided a more accurate fit. Freundlich isotherms better described the equilibrium data, indicating heterogeneous adsorption. The hydrochar obtained at 200 °C reached the highest adsorption capacity, attributed to its intact cell structure and abundance of surface functional groups. The pyrochar produced at 500 °C exhibited the highest surface area (44.3 m²/g) but a lower affinity for metribuzin due to the loss of polar functionalities during pyrolysis. This study presents a novel use of Chlorella vulgaris-derived carbon materials for metribuzin removal without chemical activation, which offers practical benefits, including simplified production, lower costs, and reduced chemical waste. The findings contribute to expanding the applicability of algae-based sorbents in water treatments, particularly where low-cost, energy-efficient materials are needed. This approach also supports the integration of carbon sequestration and wastewater remediation within a circular resource framework. Full article

The analysis of wastewater is essential in environmental chemistry, particularly for monitoring emerging contaminants and assessing ecological impacts. In this context, hyphenated chromatographic techniques are widely used, with liquid chromatography being one of the most common. However, gas chromatography coupled with mass spectrometry (GC-MS) remains a valuable tool in this field due to its sensitivity, selectivity, and widespread availability in most laboratories. This review examines the application of validated methods for wastewater analysis using GC-MS (MS), highlighting its relevance in identifying micropollutants such as pharmaceuticals, drugs of abuse, pesticides, hormones, and industrial by-products. The validation of analytical methods is crucial to ensuring the reliability and reproducibility of data and the accurate monitoring of contaminants. Key parameters, including sample volume, recovery efficiency, and detection and quantification limits, are discussed, evaluating different approaches to optimising the identification of different classes of contaminants. Additionally, this study explores advances in sample preparation techniques, such as solid-phase microextraction (SPME), dispersive liquid–liquid microextraction (DLLME), and solid-phase extraction (SPE), which enhance efficiency and minimise interferences in the analysis. Finally, future perspectives are discussed, including the integration of emerging technologies such as high-resolution mass spectrometry, the miniaturisation of GC systems, and the development of faster and more sustainable analytical methods. Full article

Stormwater carries significant amounts of pollutants—including metals, microorganisms, organic micropollutants, and nutrients—from land surfaces into nearby water bodies, leading to water quality deterioration and threats to both human health and ecosystems. The removal of these contaminants is essential not only for environmental protection, but also to enable the reuse of treated water for various beneficial applications. Common treatment methods include bioretention systems, biofiltration, constructed wetlands, rain gardens, swales, and permeable pavements. To improve pollutant removal efficiency, adsorbent materials are often incorporated into the soil substrate of these treatment devices. However, most research on adsorbents has focused on their effectiveness against one or two specific pollutants and has been conducted under static, short-term laboratory conditions rather than dynamic, field-relevant scenarios. Column-based dynamic filtration type studies, which are more informative for field applications, are limited. In one study, a combination of two or more adsorbents with contrasting properties that matched the affinity preferences of the different pollutants to the substrate media removed 77–100% of several heavy metals that occur in real stormwater compared to 38–73% removal with only one adsorbent. In another study, polycyclic aromatic hydrocarbon removal with zeolite was only 30–50%, but increased to >99% with 0.3% granular activated carbon addition. Long-term dynamic column-based filtration experiments and field studies using real stormwater, which contains a wide range of pollutants, are recommended to better evaluate the performances of the combined adsorbent systems. Full article