Search Results (205)

Syzygium oleosum (F.Muell.) B.Hyland is an Australian native species whose essential oil (EO), known commercially as “Mango Myrtle,” is gaining popularity in aromatherapy, yet remains poorly studied. This work provides the first comprehensive pharmacognostic investigation of S. oleosum. Anatomical and micromorphological analyses revealed numerous secretory cavities and calcium oxalate druses in both leaves and twigs. GC-MS analysis identified 16 components in the EO, predominantly hydrocarbon monoterpenes, with terpinolene (30.79%), β-pinene (26.79%), α-pinene (10.69%), and γ-terpinene (9.86%) as major constituents. In vitro assays showed moderate antioxidants (IC₅₀ ≤ 4.95 mg/mL) and anti-inflammatory effects (IC₅₀ ≤ 5.93 mg/mL), with specific monoterpenes contributing differentially to each activity. The EO displayed weak inhibitory activity against acetylcholinesterase (IC₅₀ 19.4 mg/mL) and butyrylcholinesterase (IC₅₀ 15.9 mg/mL), and no effect on GABA transaminase. Microelectrode array recordings on primary cortical neurons demonstrated a concentration-dependent inhibition of network activity (0.059–1.19 mg/mL) without affecting cell viability, indicating a neuromodulatory property. These results provide new insights into the pharmacological potential of S. oleosum EO and support its further evaluation as a neuroactive and anti-inflammatory agent. Full article

Brazil has one of the greatest biodiversities in the world, with emphasis on the fruit family Myrtaceae, to which the native fruits guabijú (Myrcianthes pungens (O.Berg) D.Legrand), guabiroba (Campomanesia xanthocarpa (Mart.) O.Berg), and araçá (Psidium cattleyanum Sabine) belong. These fruits are promising sources of phenolic compounds, mainly phenolic acids, flavonoids, and tannins, with high antioxidant capacity and potential health benefits. This integrative review aimed to gather and analyze data on the phenolic composition of these fruits, as well as the analytical methods used for their extraction and characterization. The reviewed literature reveals that there is still a lack of in-depth studies, although some studies have already characterized the phenolic compounds in different parts of the fruits. The efficient extraction of phenolics, especially bound ones, requires techniques such as acid or alkaline hydrolysis. For their identification and quantification, the use of liquid chromatography (LC) coupled to mass spectrometry (MS) with mass analyzers such as triple quadrupole (QqQ) and quadrupole-time-of-flight (QToF), stands out. Knowledge of these fruits contributes to the development of functional ingredients and the conservation and appreciation of Brazilian biodiversity, thereby reinforcing the importance of expanding research on these fruits and exploring potential applications in the food, pharmaceutical, and cosmetic industries. Full article

Objectives: This study analyzed the antiproliferative potential of Eugenia uniflora L. leaf extracts against cervical cancer and non-cancerous cell lines. Methods: The extracts were prepared by maceration using hexane (EUH), dichloromethane (EUD), and ethyl acetate (EUA). Their cytotoxic potential was evaluated through MTT assays, wound healing assays, and flow cytometry. To identify classes of secondary metabolites, total phenolic and flavonoid contents were quantified using spectrophotometric methods, and individual metabolites were tentatively identified by LC-MS/MS. Results: EUH, EUD. and EUA exhibited cytotoxicity in HeLa cells, with IC₅₀ values of 63.03 μg/mL, 33.79 μg/mL, and 38.38 μg/mL, respectively. Due to their lower IC₅₀ values, the EUD and EUA fractions were selected for further investigation. EUA and EUD inhibited cell migration at all the time points tested and altered the cell cycle. Twenty-eight compounds were tentatively identified in E. uniflora L. leaf extracts based on the interpretation of their fragmentation patterns and molecular formulas obtained from mass spectrometry. Conclusions: The EUD and EUA extracts appear to modulate the metabolism of cervical cancer cells, leading to cell cycle arrest and inhibition of cell migration. Flavonoids and other phenolic compounds are likely responsible for these observed biological effects. Full article

Accurate estimation of aboveground biomass (AGB) in tree–shrub communities is critical for quantifying forest ecosystem productivity and carbon sequestration potential. Although generalized allometric equations offer expediency in natural forest AGB estimation, their neglect of interspecific variability introduces methodological pitfalls. Precise AGB prediction necessitates resolving two biological constraints: phylogenetic conservation of allometric coefficients and ontogenetic regulation of scaling relationships. This study establishes an integrated framework combining the following: (1) phylogenetic signal detection (Blomberg’s K/Pagel’s λ) across 157 species’ allometric equations, revealing weak but significant evolutionary constraints (λ = 0.1249, p = 0.0027; K ≈ 0, p = 0.621); (2) hierarchical error decomposition of 9105 stems in a Mt. Wuyishan forest dynamics plot (15 species), identifying family-level error stratification (e.g., Theaceae vs. Myrtaceae, Δerror > 25%); (3) ontogenetic trajectory analysis of Castanopsis eyrei between Mt. Wuyishan and Mt. Huangshan, demonstrating significant biomass deviations in small trees (5–15 cm DBH, p < 0.05). Key findings resolve the following hypotheses: (1) absence of strong phylogenetic signals validates generalized models for phylogenetically diverse communities; (2) ontogenetic regulation dominates error magnitude, particularly in early developmental stages; (3) differential modeling is recommended: species-specific equations for pure forests/seedlings vs. generalized equations for mixed mature forests. This work establishes an error hierarchy: ontogeny > taxonomy > phylogeny, providing a mechanistic basis for optimizing forest carbon stock assessments. Full article

Saninten (Castanopsis argentea (Blume) A.DC.) is a protected plant that grows in the Mount Gede Pangrango National Park (MGPNP) area in West Java. Its population is limited, and as a valuable biological resource, Castanopsis has traditionally been utilized by indigenous communities, particularly those residing in proximity to the forest. However, the expansion and development of tourism pose a potential threat to the ecosystems of C. argentea and other endemic plant species, as well as to the wildlife that depend on these habitats. Comprehensive data on biodiversity, species composition, forest structure, and carbon stock status are crucial for assessing the potential impact of future tourism development. Our investigation was conducted from November 2023 to March 2024 in a three-hectare utilization zone within the confines of the national park. The findings documented a total of 36 species across 23 distinct plant families, with the families Fagaceae, Moraceae, and Myrtaceae exhibiting the highest levels of dominance. The regeneration of stands at the study site predominantly comprised arboreal species with the most substantial carbon stocks, including C. acuminatissima (Blume) A.DC. (Riung anak), C. argentea (Saninten), and Litsea sp. (Huru). C. argentea supplies several functions within this ecosystem that are interconnected with other components. With aboveground carbon stocks reaching 560.47 tons C/ha, the forest demonstrates high sequestration potential, reinforcing the need to conserve mature stands for both biodiversity and climate benefits. Therefore, in the future, the conservation of C. argentea will benefit the maintenance of the ecosystem’s attractiveness without adversely affecting the social and cultural structures of the local population. Full article

Eucalypts are a diverse group of Myrtaceae native to Australia and adapted to a wide range of edaphoclimatic conditions, including variation in phosphorus (P) soil availability. While Corymbia and Eucalyptus species have evolved in P-poor soils, they still respond to P additions. Nutrient ratios have been used to study nutritional imbalances in plants, as they relate to nutrient homeostasis within cells and ultimately productivity. This study investigated the effects of providing adequate (normal) and high doses of phosphorus (P) on nutrient ratios in leaves and stems of Eucalyptus and Corymbia species. High soil P may happen due to high natural soil concentration and over-fertilization. These species were pre-selected from a 22-eucalypt species screening, based on their responses—either positive, negative, or neutral—to increased dry mass at high soil P compared to normal P. Two species, Corymbia citriodora and C. maculata, which showed increased dry mass under high P levels, exhibited enhanced shoot growth and improvements in parameters related to photosystem efficiency. Except for Zn, which has an antagonistic relationship with P, the concentrations of other nutrients known to exhibit either antagonism or synergism with P were not significantly altered in the leaves and stems. As a result, there were no notable changes in the ratios with high P data compared to those with normal P data. Ratios calculated among K, Ca, Mg, Fe, and Mn data also remained unchanged. However, a principal component analysis, which was performed with all nutrient ratios, effectively separated the normal P and high P treatments and distinguished between species belonging to the genera Corymbia and Eucalyptus. The validity of such nutrient ratios is discussed, and it is suggested that they may not be applicable in studies involving high nutrient doses, which may also be true for other nutrients. Additionally, using ratios under unbalanced field fertilization may lead to an incorrect nutritional interpretation. Full article

Background: Eugenia involucrata DC., a Cerrado native plant, is recognized for its medicinal properties. However, its bioactive compounds remain inadequately explored. Objectives: This study investigated bioactive compounds from a standardized liquid extract from E. involucrata leaves that can act with antioxidant, cytogenotoxic, cytoprotective, and genoprotective effects. Methods: The phenolic compounds in the standardized liquid extract from E. involucrata leaves were screened by HPLC-DAD. The capture of the free radicals DPPH, ABTS⁺, and the metal reduction power FRAP determined the antioxidant potential. Cytotoxicity was evaluated in RAW 264.7 macrophages (MTT assay), and (anti)cytotoxic and (anti)genotoxic effects were assessed in human lymphocytes using the Trypan blue exclusion method and comet assay, respectively. Results: The extracts present key phenolic compounds, such as ellagic acid, myricitrin, and epicatechin gallate. The standardized extract demonstrated antioxidant capacity, evidenced by its ability to reduce iron and scavenge free radicals. The liquid extract from E. involucrata leaves exhibited cytotoxic effects on RAW 264.7 macrophages at higher concentrations, while demonstrating (anti)cytotoxic activity on human lymphocytes from all tested concentrations. The highest concentration tested of the standardized liquid extract from E. involucrata leaves (250 µg/mL) showed genotoxicity against human lymphocytes compared to the negative control. In contrast, the lowest concentration (62.5 µg/mL) exhibited an antigenotoxic effect on human lymphocytes, reducing the genotoxicity of doxorubicin by approximately 27%. Conclusions: The bioactive compounds in the standardized liquid extract from E. involucrata leaves exhibited antioxidant and antigenotoxic properties, suggesting potential value for nutraceutical and pharmaceutical applications, particularly those related to oxidative stress associated withaging and disease progression. Full article

Eucalyptus camaldulensis of the Myrtaceae family shows high resistance to aluminum (Al) ions and contains various compounds such as steroids, terpenoids, saponins, flavonoids, glycosides, alkaloids, and tannins. Although the ellagitannin oenothein B (12) isolated from E. camaldulensis exhibits remarkable properties for Al detoxification, likely contributing to its Al resistance, other ellagitannin oligomers present in E. camaldulensis have not been investigated in detail. In this study, novel dimeric and trimeric ellagitannin oligomers eucarpanin D₂ (1) and eucamalin A (2), together with known gallotannins (7, 8, and 10), monomeric ellagitannins (4–6, and 11), and dimeric ellagitannins (3, 9, and 12–14), were isolated from E. camaldulensis leaves. The structures of these novel compounds were elucidated based on their chemical and physicochemical properties, including the orientations of tergalloyl groups in compounds 1 and 2. Similar to compound 12, previously isolated from the roots of E. camaldulensis, the ellagitannins demonstrated good Al detoxification properties. Hence, these tannins may play a critical role in the high Al resistance of E. camaldulensis in acidic soils. This paper reports for the first time the isolation of ellagitannin oligomers from the leaves of E. camaldulensis. Full article

The aquarium trade includes a niche but significant market for ornamental wood, yet its sustainability remains largely unexamined. This study combines traditional wood anatomical methods with conservation assessments to investigate the sustainability of this overlooked sector. We investigated the botanical identity of aquarium wood products to assess mislabelling, conservation concerns, and potential environmental risks. Using macroscopic and microscopic wood anatomy techniques, we analysed wood samples from the aquarium trade to determine taxonomic identity. We also examined the origin, conservation status, and possible ecological implications of the identified taxa. We identified woods from diverse families, including Myrtaceae, Ericaceae, Fabaceae, and Ebenaceae, with some species listed as threatened. Widespread mislabelling obscures conservation status, enabling unsustainable trade. Moreover, several samples were identified as roots, raising concerns about soil disruption and habitat degradation. By linking wood anatomy and ecosystem impacts, we show that the aquarium wood trade lacks transparency, posing risks to biodiversity conservation and ecosystem stability. Our findings underscore the pressing need for effective regulatory oversight, accurate labelling, and sustainable sourcing to mitigate environmental impacts and promote responsible trade practices. Full article

The guava tree (Psidium guajava L.) is a tropical plant from the Myrtaceae family. Leaf extracts from this plant have been used in traditional medicine to treat gastrointestinal disorders and exhibit several functional activities that benefit human health. Different varieties of guava trees produce fruits in colors ranging from white to red and present a characteristic metabolic profile in both their leaves and fruits. This study presents a metabolomic characterization of the leaves from two guava varieties: the Caxcana cultivar with yellow fruits and the S-56 accession with pink fruits. Metabolite profiling was conducted using Gas Chromatography–Mass Spectrometry (GC–MS) on methanol extracts, followed by multivariate statistical analysis, including Principal Component Analysis (PCA), and a heat map visualization of compound concentrations in the two varieties. The results identified β-caryophyllene as the major secondary metabolite present in both varieties, with a relative abundance of 16.46% in the Caxcana variety and 23.06% in the S-56 cultivar. Furthermore, in silico analyses, such as network pharmacology and molecular docking, revealed key interactions with proteins such as CB2, PPARα, BAX, BCL2, and AKT1, suggesting potential therapeutic relevance. These findings highlight the pharmacological potential of guava leaf metabolites in natural product chemistry and drug discovery. Full article

By-products of fruit processing may contain bioactive compounds with potential application as bioinputs. This study optimized the ultrasound-assisted extraction (UAE) of phenolic compounds from the by-products of Eugenia dysenterica DC (Myrtaceae) fruit to obtain bioinputs with antioxidant, antimicrobial, and antibiofilm activities. The fruit by-products (seeds and peels) were subjected to UAE optimization using the Box–Behnken design and response surface methodology. Two optimal conditions were defined: 1-plant material/solvent ratio of 0.01 g/mL, time of 40 min, and ethanol content of 30%; 2–0.19 g/mL, 39 min, and 46%. The bioinputs (liquid extract—LQE; lyophilized extract—LYE), obtained under the optimal conditions, were tested for antioxidant activity (ABTS, FRAP, and DPPH). LQE: 1633.13 µM Trolox/g, 1633.60 µM FeSO₄/g and 73.35 g sample/g DPPH; LYE: 1379.75 µM Trolox/g, 1692.09 µM FeSO₄/g and 83.35 g sample/g DPPH. For antimicrobial activity, both extracts presented MBC < 62.5 mg/mL and MIC and MBC of 2.5 mg/mL for P. aeruginosa. LQE presented antibiofilm action for S. coagulase (50 mg/mL) and Streptococcus spp. (12.5 mg/mL); LYE for P. aeruginosa (50 mg/mL; 12.5 mg/mL), E. coli (25 mg/mL). The bioinputs obtained by UAE under optimized conditions for phenolic compounds present antioxidant, antimicrobial, and antibiofilm activities. Full article

Rhodomyrtus psidioides (G.Don) Benth. (Myrtaceae) is a critically endangered rainforest species from the east coast of Australia, where populations have severely and rapidly declined due to the effects of repeated myrtle rust infection. With very limited material available in the wild and freezing-sensitive seeds that have prevented storage in a seed bank, ex situ conservation of this exceptional species has proven difficult. Material from a seed orchard grown at the Australian Botanic Garden Mount Annan was successfully used to initiate three new accessions into tissue culture from cuttings, and to undertake cryopreservation experiments using a droplet-vitrification (DV) protocol for both seeds and cultured shoot tips. Use of seedling material for tissue culture initiation was very effective, with a 94–100% success rate for semi-hardwood explants and a 50–62% success rate for softwood explants. Although no survival of seeds after cryopreservation was observed, seeds of R. psidioides showed some tolerance of desiccation and exposure to cryoprotective agents. Regeneration after cryopreservation using a DV protocol was demonstrated in only one shoot tip precultured on basal medium containing 0.4 M sucrose and incubated in PVS2 for 20 min prior to immersion in liquid nitrogen. These results demonstrate the value of living collections in botanic gardens for conservation research, highlight the importance of germplasm choice for tissue culture initiation, and demonstrate the potential of cryobiotechnologies for the ex situ conservation of exceptional plant species. Full article

South American wood and wood-based products play major roles in the global forest sector. Most research related to Paraguayan wood is focused on forest restoration, urban arborization, silviculture, and botanical taxonomy. Often overlooked but of major importance is the cellular structure of the trees that comprise remaining forests in Paraguay. Wood greatly contributes to forest value, yet wood anatomy studies remain novel in the country. To further document Paraguayan wood anatomy, two downed species of multipurpose Myrtaceae trees were sampled from a subtropical semi-deciduous forest in Areguá, Central Paraguay. In this article, heartwood xylem anatomy was observed and documented using low-cost methodology to support the regional realities of the emerging field in rural communities, especially local Paraguayan peoples. This included specific gravity, density, and basic light microscopic features. Sample material was processed near the pith at breast height to display cellular features in the transverse, radial, and tangential planes. Four features were measured with light microscopy and ImageJ: tangential vessel element diameter, vessel element length, ray seriation, and ray height. Results showed structural similarity between species, with diffuse porosity, solitary pores, simple perforation plates, alternate intervessel pits, and apotracheal diffuse parenchyma in aggregates. These results represent the first sampling of Myrtaceae from Paraguay in a methodology that can be easily replicated by the native population, thereby enabling further wood anatomy studies in the region. Full article

Myrtle (Myrtus communis L.), an evergreen shrub belonging to the Myrtaceae family, is widely valued for its applications in the cosmetic, hygienic, and medicinal industries. This plant produces berries in two colors—white and black—with black berries receiving increasing attention due to their superior antioxidant properties. This study investigates black myrtle berries’ morphological and biochemical characteristics from eight prominent natural habitats in Fars Province, southwestern Iran. The results reveal significant variations in the morphological (such as fruit length and diameter, fruit length-to-diameter ratio, seed length and diameter, fruit weight, pulp weight, seed weight, pulp-to-seed ratio, and number of seeds) and biochemical attributes among the studied populations. The longest fruit was from the Kherqeh (KH) (8.29 mm) population, while the shortest was from the Baghnari (BN) (5.85 mm) population. The largest fruit diameter was also from KH (6.83 mm), which did not show a significant difference compared to the Zanjiran (ZF) population, while the smallest fruit diameter was from BN (5.12 mm), which did not differ significantly from the Kavar (KA), Simakan (SM), Kouhmareh Road (JK), or Atashkadeh (AT) populations. Notably, all populations exhibited high levels of phenolic compounds, ranging from 660 to 1846 mg per 100 g of fresh weight, and potent antioxidant activity, as indicated by low half-maximal inhibitory concentrations ranging from 0.018 to 0.187 mg per gram. Correlation analyses further demonstrated that altitude and specific soil properties influenced the biochemical traits of the berries to varying degrees. These findings offer valuable scientific insights for selecting and utilizing specific myrtle populations in horticulture, particularly for breeding programs to maximize antioxidant properties and phenolic content. Full article

Syzygium cumini (L.) Skeels, commonly known as the Jamun or Indian blackberry, is a tropical evergreen tree native to the Indian subcontinent, and it belongs to the Myrtaceae family. This research aimed to assess the antibacterial properties of the extracts derived from S. cumini seed kernels and evaluate their total flavonoid content, total phenol content, total carbohydrate content, antioxidant capacity, and inhibitory effects on xanthine oxidase. Cold maceration was chosen for its ability to preserve thermolabile compounds and efficiently extract bioactive constituents with minimal energy and equipment requirement, with hexane and methanol employed as extraction solvents. The methanolic seed kernel extract of S. cumini showed the highest flavonoid (127.78 μg quercetin equivalent/mg dried extract vs. 21.24 μg quercetin equivalent/mg in hexane dried extract) and polyphenol content (153.81 μg gallic acid equivalent/mg dried extract vs. 38.89 μg gallic acid equivalent/mg in hexane dried extract), along with significant carbohydrate content (475.61 μg glucose equivalent/mg dried extract vs. 5.57 μg GE/mg in hexane dried extract). It also demonstrated potent antioxidant activity (IC₅₀: 9.23 μg/mL; ascorbic acid: 5.10 μg/mL) and xanthine oxidase inhibition (IC₅₀: 14.88 μg/mL), comparable to the standard drug allopurinol (IC₅₀: 6.54 μg/mL), suggesting its therapeutic potential. Moreover, the methanolic extract of seed kernels exhibited strong antibacterial activity, with inhibition zones of 19.00 mm against S. epidermidis, higher than the standard antibiotic (gentamicin: 18.33 mm) against K. pneumonia (ciprofloxacin: 33.66 mm). The lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 0.32 mg/mL and 0.52 mg/mL, respectively, were observed for the same extract against S. epidermis. In conclusion, this study demonstrated the remarkable antibacterial effects of S. cumini methanolic seed kernel extract against various pathogenic microorganisms as well as significant inhibitory effects on xanthine oxidase and antioxidant activity. Full article