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15 pages, 4909 KB  
Article
Acid Adaptation Leads to Sensitization of Salmonella Challenge Cultures During Processing of Air-Dried Beef (Biltong, Droëwors)
by Pratikchhya Adhikari, Cailtin E. Karolenko, Jade Wilkinson and Peter M. Muriana
Appl. Microbiol. 2025, 5(4), 106; https://doi.org/10.3390/applmicrobiol5040106 - 6 Oct 2025
Abstract
US food regulatory agencies have adopted a preference for researchers and testing labs to use ‘acid adapted challenge cultures’ when performing inoculated validation studies of food processes that involve acidic treatments to accustom the cultures to acidic pH so that they will not [...] Read more.
US food regulatory agencies have adopted a preference for researchers and testing labs to use ‘acid adapted challenge cultures’ when performing inoculated validation studies of food processes that involve acidic treatments to accustom the cultures to acidic pH so that they will not be easily affected during processing. We evaluated acid adaptation in regard to the processing of South African style air-dried beef, notably biltong and droëwors, using a mixture of five serovars of Salmonella as well as a unique serovar isolated from dried beef (Salmonella Typhimurium 1,4,[5],12:i:-). Acid adaptation was obtained by growing cultures in tryptic soy (TS) broth containing 1% glucose. Non adapted cultures were obtained by growth in TS broth without glucose or in TS broth with 1% glucose but buffered with 0.2 M phosphate buffer. Processes included biltong (dried solid beef) and droëwors (ground, sausage-style). Each trial was performed twice and triplicate samples were examined at each sampling point (i.e., n = 6). Statistical analysis was applied using analysis of variance (ANOVA) or one-way repeated measures (RM-ANOVA) and the Holm–Sidak test for pairwise multiple comparisons to determine significant differences (p < 0.05). We observed that in all processes examined (eight trials), treatments using acid adapted cultures were more sensitive to the biltong and droëwors processes, giving greater reductions (5.3-log reduction) than when non-adapted cultures were used (3.8-log reduction). Acid adaptation leads to stressed conditions in Salmonella resulting in sensitization to the multiple hurdles found in biltong and droëwors processing (acid/vinegar, salt, desiccation). Based on our data, the use of non adapted Salmonella cultures to achieve desired challenge culture process lethality could result in more robust processing conditions and a greater level of safety in these products as intended by US regulatory guidance. Full article
(This article belongs to the Special Issue Applied Microbiology of Foods, 3rd Edition)
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11 pages, 523 KB  
Review
Physical Activity During Pregnancy and Gestational Weight Gain: Implications for Maternal–Fetal Epigenetic Programming and Long-Term Health
by Nektaria Zagorianakou, Stylianos Makrydimas, Efthalia Moustakli, Ioannis Mitrogiannis, Ermanno Vitale and George Makrydimas
Genes 2025, 16(10), 1173; https://doi.org/10.3390/genes16101173 - 6 Oct 2025
Abstract
Background/Objectives: Gestational weight gain (GWG) is a crucial factor influencing mother and fetal health, as high GWG is associated with adverse pregnancy outcomes and an increased long-term risk of obesity and metabolic issues in the children. In addition to controlling weight, maternal [...] Read more.
Background/Objectives: Gestational weight gain (GWG) is a crucial factor influencing mother and fetal health, as high GWG is associated with adverse pregnancy outcomes and an increased long-term risk of obesity and metabolic issues in the children. In addition to controlling weight, maternal physical activity (PA) during pregnancy may influence fetal development through potential epigenetic mechanisms, including histone modifications, DNA methylation, and the production of non-coding RNA. Methods: This narrative review synthesizes evidence from randomized controlled trials (RCTs; n = 11, 3654 participants) investigating the impact of aerobic PA on GWG, while also highlighting emerging, primarily indirect findings on maternal–fetal epigenetic programming. Results: The majority of RCTs found that supervised PA interventions, especially when paired with nutritional counseling, decreased both the incidence of excessive GWG and total GWG. Enhancements in lipid metabolism, adipokine profiles, and maternal insulin sensitivity point to likely biochemical mechanisms that connect PA to epigenetic modification of fetal metabolic genes (e.g., IGF2, PGC-1α, LEP). Animal and observational studies suggest that maternal activity may influence offspring epigenetic pathways related to obesity and cardiometabolic conditions, although direct human evidence is limited. Conclusions: In addition to potentially changing gene–environment interactions throughout generations, prenatal PA is a low-cost, safe method of improving maternal and newborn health. Future RCTs ought to incorporate molecular endpoints to elucidate the epigenetic processes by which maternal exercise may provide long-term health benefits. Full article
(This article belongs to the Section Epigenomics)
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19 pages, 5356 KB  
Article
Bisphenol AF Induces Hepatic Steatosis via Succinate–SUCNR1-Mediated Macrophage–Hepatocyte Interactions: An Adverse Outcome Pathway Study in Male C57BL/6 Mice
by Ning Wang, Jing Leng, Huimin Zhang, Jing Xu, Xiaoqi Yu, Kelei Qian, Zhiqing Zheng, Mengchao Ying, Gonghua Tao, Ping Xiao and Xinyu Hong
Int. J. Mol. Sci. 2025, 26(19), 9720; https://doi.org/10.3390/ijms26199720 (registering DOI) - 6 Oct 2025
Abstract
Bisphenol AF (BPAF) exposure is increasingly linked to metabolic disorders, yet the molecular initiating events (MIE) and key events (KE) leading to hepatic steatosis remain unclear. We constructed an adverse outcome pathway (AOP) to mechanistically connect BPAF-triggered macrophage–hepatocyte crosstalk to liver fat accumulation. [...] Read more.
Bisphenol AF (BPAF) exposure is increasingly linked to metabolic disorders, yet the molecular initiating events (MIE) and key events (KE) leading to hepatic steatosis remain unclear. We constructed an adverse outcome pathway (AOP) to mechanistically connect BPAF-triggered macrophage–hepatocyte crosstalk to liver fat accumulation. Male C57BL/6 mice received daily oral gavage of 0, 0.5, 4, or 32 mg kg−1 BPAF for 90 d, and Transwell co-cultures of RAW264.7 macrophages and AML12 hepatocytes were used for in vitro validation. Targeted metabolomics, western blotting, and lipid staining quantified succinate, pathway proteins, and steatosis. BPAF dose-dependently increased serum succinate (BMD = 6901.95 nM) and hepatic triglyceride (TG) (BMD = 874.26 nM). Cryo-EM docking revealed BPAF binding to SUCNR1 at 2.9 Å, disrupting the inactive-state conformation. In co-culture, BPAF-exposed macrophages released succinate that bound hepatocyte SUCNR1, suppressed Akt phosphorylation, and activated JNK. These KEs led to a 40% increase in lipid droplets and elevated TG, total cholesterol (TC), and free fatty acids (FFA) without liver weight gain. We propose the first AOP for BPAF-induced hepatic steatosis: BPAF–SUCNR1 binding (MIE) → macrophage succinate release (KE1) → SUCNR1-mediated Akt inhibition/JNK activation (KE2–4) → hepatic lipid accumulation (KE5) → steatosis (AO). These findings provide mechanistic insight for chemical risk assessment of BPAF and structurally related bisphenols. Full article
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
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13 pages, 1160 KB  
Article
MicroRNA-371a-3p Represents a Novel and Effective Diagnostic Marker for Testicular Germ Cell Tumours: A Real-World Prospective Comparison with Conventional Approaches
by Margherita Palermo, Carolina D’Elia, Giovanni Mazzucato, Christine Mian, Christine Schwienbacher, Esther Hanspeter, Silvia Clauser, Salvatore Mario Palermo, Armin Pycha, Isabel Heidegger, Igor Tsaur and Emanuela Trenti
Pathophysiology 2025, 32(4), 54; https://doi.org/10.3390/pathophysiology32040054 (registering DOI) - 6 Oct 2025
Abstract
Background/Objectives: Testicular germ cell tumours (GCT) have high cure rates, especially in early stages. MicroRNA-371a-3p (M371) has recently emerged as a highly sensitive biomarker for malignant GCTs, except teratoma. This study aimed to evaluate the diagnostic performance of M371-test in a real-life clinical [...] Read more.
Background/Objectives: Testicular germ cell tumours (GCT) have high cure rates, especially in early stages. MicroRNA-371a-3p (M371) has recently emerged as a highly sensitive biomarker for malignant GCTs, except teratoma. This study aimed to evaluate the diagnostic performance of M371-test in a real-life clinical setting, compared to conventional markers alpha-fetoprotein (AFP), lactate-dehydrogenase (LDH), and beta-human chorionic gonadotropin (β-HCG) in patients with suspected GCT. Methods: The study, approved by the Ethic-Committee of the Provincial Hospital of Bolzano (N.97-2021), included 91 M371-tests, performed from March 2021 to May 2025. A total of 75 patients had suspected GCT; 19 healthy males served as control. Serum levels of M371, AFP, LDH, and β-HCG were compared with final histopathological diagnosis. M371 was also assessed in controls to evaluate test performance. Secondary analyses investigated correlations between preoperative M371 levels and tumour size in non-metastatic patients, and between M371-levels and clinical stage in the entire GCT cohort. A cut-off of RQ > 5 (relative quantification) was used to calculate sensitivity, specificity, and predictive values. Results: M371 showed a sensitivity of 90.9% and specificity of 89.3%, outperforming in terms of sensitivity AFP (20.4%/96.4%), LDH (40.9%/96.4%), and β-HCG (43.1%/100%). Positive predictive value (PPV) and negative predictive value (NPV) were 93.0% and 86.2%, respectively. Sensitivity was 95% for non-seminomas and 87.5% for seminomas. In non-metastatic patients, M371 levels correlated with tumour size and were significantly higher in advanced stages (median RQ 1128.35 vs. 98.36; p = 0.015). Conclusions: M371 showed excellent diagnostic performance, even for small tumours, supporting its clinical use. Further studies are needed to define its role in treatment planning and follow-up. Full article
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18 pages, 3967 KB  
Article
Enhanced Piezoelectric and Ferroelectric Properties in the Lead-Free [(BiFeO3)m/(SrTiO3)n]p Multilayers by Varying the Thickness Ratio r = n/m and Periodicity p
by Jonathan Vera Montes, Francisco J. Flores-Ruiz, Carlos A. Hernández-Gutiérrez, Enrique Camps, Enrique Campos-González, Gonzalo Viramontes Gamboa, Fernando Ramírez-Zavaleta and Dagoberto Cardona Ramírez
Coatings 2025, 15(10), 1170; https://doi.org/10.3390/coatings15101170 - 6 Oct 2025
Abstract
Multilayer heterostructures of [(BiFeO3)m/(SrTiO3)n]p were synthesized on ITO-coated quartz substrates via pulsed laser deposition, with varying thickness ratios (r = n/m) and periodicities (p = 1–3). Structural, electrical, and piezoelectric properties were systematically [...] Read more.
Multilayer heterostructures of [(BiFeO3)m/(SrTiO3)n]p were synthesized on ITO-coated quartz substrates via pulsed laser deposition, with varying thickness ratios (r = n/m) and periodicities (p = 1–3). Structural, electrical, and piezoelectric properties were systematically investigated using X-ray diffraction, AFM, and PFM. The BiFeO3 layers crystallized in a distorted rhombohedral phase (R3c), free of secondary phases. Compared to single-layer BiFeO3 films, the multilayers exhibited markedly lower leakage current densities and enhanced piezoelectric response. Electrical conduction transitioned from space-charge-limited current at low fields (E < 100 kV/cm) to Fowler–Nordheim tunneling at high fields (E > 100 kV/cm). Optimal performance was achieved for r = 0.30, p = 1, with minimal leakage (J = 8.64 A/cm2 at E = 400 kV/cm) and a peak piezoelectric coefficient (d33 = 55.55 pm/V). The lowest coercive field (Ec = 238 kV/cm) occurred in the configuration r = 0.45, p = 3. Saturated hysteresis loops confirmed stable ferroelectric domains. These findings demonstrate that manipulating layer geometry in [(BiFeO3)m/(SrTiO3)n]p stacks significantly enhances functional properties, offering a viable path toward efficient, lead-free piezoelectric nanodevices. Full article
(This article belongs to the Special Issue Thin Films and Nanostructures Deposition Techniques)
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7 pages, 1156 KB  
Communication
Gas Phase Fragmentation of N,N-Ditosyl-2-aminodiphenylamine to Phenazine
by M. John Plater and William T. A. Harrison
Molbank 2025, 2025(4), M2069; https://doi.org/10.3390/M2069 (registering DOI) - 6 Oct 2025
Abstract
N,N-Ditosyl-2-aminodiphenylamine was prepared by the tosylation of 2-aminodiphenylamine with tosylchloride in dichloromethane. Unwanted isomers owing to the tosylation of the diarylamine were not formed. This compound was fully characterized by IR, UV/Vis, NMR, m/z, and mp, including an X-Ray [...] Read more.
N,N-Ditosyl-2-aminodiphenylamine was prepared by the tosylation of 2-aminodiphenylamine with tosylchloride in dichloromethane. Unwanted isomers owing to the tosylation of the diarylamine were not formed. This compound was fully characterized by IR, UV/Vis, NMR, m/z, and mp, including an X-Ray single crystal structure determination. It was fragmented in an Atmospheric Solids Analysis Probe (ASAP) mass spectrometer showing a series of fragments down to phenazine. Full article
(This article belongs to the Section Organic Synthesis and Biosynthesis)
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15 pages, 1239 KB  
Article
Effects of Respiratory Muscle Training on Performance and Inspiratory Strength in Female CrossFit Athletes: A Randomized Controlled Trial
by Juliana Andrade Assis, Lúcio Marques Vieira-Souza, Diego Valenzuela Pérez, Cristiano Diniz da Silva, Carlos Fuentes Veliz, Naiara Ribeiro Almeida, Bianca Miarka, Otávio Toledo Nóbrega and Ciro José Brito
Physiologia 2025, 5(4), 39; https://doi.org/10.3390/physiologia5040039 (registering DOI) - 6 Oct 2025
Abstract
Background: The high-intensity demands of CrossFit induce respiratory muscle fatigue, potentially impairing performance via the metaboreflex. Respiratory muscle training (RMT) may mitigate this effect, but evidence in female athletes remains limited. Objective: We aimed to investigate the effects of RMT on sport-specific performance [...] Read more.
Background: The high-intensity demands of CrossFit induce respiratory muscle fatigue, potentially impairing performance via the metaboreflex. Respiratory muscle training (RMT) may mitigate this effect, but evidence in female athletes remains limited. Objective: We aimed to investigate the effects of RMT on sport-specific performance and maximal inspiratory pressure (PIMAX) in recreational female CrossFit practitioners. Design: We conducted a parallel-group randomized controlled trial. Setting: The study was conducted in a CrossFit-affiliated gym. Participants: We recruited twenty-nine recreational female practitioners (age: 30.3 ± 7.9 years) with ≥1 year of uninterrupted training who were free from respiratory diseases. Interventions: Participants were randomized to a CrossFit-only group (n = 14) or CrossFit + RMT group (n = 15). Both trained 5 days/week for 6 weeks; the RMT group additionally performed 30 inspiratory efforts at 50% of PIMAX, 5 days/week, with weekly load adjustment. Main Outcome Measures: Primary: Sport-specific performance (total repetitions in a 10-min AMRAP [As Many Rounds As Possible] test). Secondary: PIMAX (cmH2O). Measurements were taken pre- and post-intervention. Results: Baseline performance and PIMAX were similar between groups. After 6 weeks, the CrossFit + RMT group improved in performance more (Δ = +10.5 ± 10.7 reps, p = 0.03, ηp2 = 0.168) than the CrossFit-only group (Δ = +2.3 ± 8.1). PIMAX gains were also greater with RMT (Δ = +19.6 ± 8.4 cmH2O, p = 0.043, ηp2 = 0.148) vs. control (Δ = +10.1 ± 9.7). No adverse events occurred. Conclusions: Adding RMT to CrossFit training enhanced sport-specific performance and inspiratory strength in recreational female practitioners more than CrossFit alone. RMT appears to be a safe and effective complementary strategy for high-intensity functional training. Full article
(This article belongs to the Special Issue Exercise Physiology and Biochemistry: 3rd Edition)
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15 pages, 2901 KB  
Article
Enhanced Nitrification Potential Soil from a Warm-Temperate Shrub Tussock Ecosystem Under Nitrogen Deposition and Warming Is Driven by Increased Nitrosospira Abundance
by Baihui Ren, Longzhen Ma, Tianyue Xu, Haoyan Li, Jiahuan Li, Jiyun Yang and Long Bai
Agronomy 2025, 15(10), 2347; https://doi.org/10.3390/agronomy15102347 - 6 Oct 2025
Abstract
Atmospheric nitrogen (N) deposition and climate warming significantly influence soil nitrogen transformation processes. Nitrification, a key step in the N cycle, is primarily driven by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). However, their responses to environmental changes in warm-temperate shrub tussock grasslands—a [...] Read more.
Atmospheric nitrogen (N) deposition and climate warming significantly influence soil nitrogen transformation processes. Nitrification, a key step in the N cycle, is primarily driven by ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB). However, their responses to environmental changes in warm-temperate shrub tussock grasslands—a major grassland type in China—remain poorly understood. In this study, we examined the effects of N addition and warming on the community composition of ammonia oxidizers and soil nitrification potential (NP) through pot experiments simulating field conditions. Our results demonstrated that (1) the AOB community was more responsive to N addition and warming than AOA, with the genus Nitrosospira increasing by 6.30–21.75% under treatments; (2) soil pH increased significantly under warming (from 6.53 to 6.86) but remained unchanged under N addition; (3) NP increased significantly under all treatment conditions, most markedly under warming alone (2.83-fold increase compared to the control); and (4) NP was positively correlated with both soil pH and the relative abundance of Nitrosospira. These findings suggest that warming and N deposition enhance nitrification in shrub tussock soil by altering AOB community structure and increasing soil pH. This study provides new insights into the microbial mechanisms driving N cycling in warm-temperate grasslands under global change. Full article
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18 pages, 1360 KB  
Article
Mechanical Versus Restrictive Kinematic Alignment in Robotic-Assisted Total Knee Arthroplasty: A Randomized Controlled Trial
by Alexey V. Lychagin, Andrey A. Gritsyuk, Mikhail P. Elizarov, Andrey A. Gritsuk, Maxim Y. Gavlovsky, Konstantin K. Tomboidi, Eugene B. Kalinsky and Nahum Rosenberg
Diagnostics 2025, 15(19), 2524; https://doi.org/10.3390/diagnostics15192524 - 6 Oct 2025
Abstract
Background: Lower limb malalignment is a hallmark of knee osteoarthritis, with surgical correction techniques evolving from traditional mechanical alignment (MA) to kinematic alignment (KA) approaches. Restrictive kinematic alignment (rKA) represents a hybrid strategy combining principles from both techniques. This study evaluated short-term [...] Read more.
Background: Lower limb malalignment is a hallmark of knee osteoarthritis, with surgical correction techniques evolving from traditional mechanical alignment (MA) to kinematic alignment (KA) approaches. Restrictive kinematic alignment (rKA) represents a hybrid strategy combining principles from both techniques. This study evaluated short-term functional outcomes following robotic-assisted total knee arthroplasty (RoTKA), comparing MA versus rKA alignment strategies. Methods: This prospective, randomized, single-center study enrolled 96 patients with grade 3–4 idiopathic knee osteoarthritis (Kellgren–Lawrence classification). Patients were randomized to MA (n = 49, mean age 67 ± 9 years) or rKA (n = 47, mean age 66 ± 7 years) groups. Preoperative hip–knee–ankle (HKA) angles were 172.6° ± 1.1° and 172.9° ± 0.9° for MA and rKA groups, respectively. Outcomes were assessed using Visual Analog Scale (VAS) pain scores, range of motion (ROM), Knee Society Score (KSS), Oxford Knee Score (OKS) (primary outcome), SF-36, and Forgotten Joint Score (FJS-12). Results: Postoperative HKA angles were 179.5° ± 1.2° (MA) and 176.0° ± 1.5° (rKA). At 14 days postoperatively, knee ROM increased by 20.5% in the MA group and 25.7% in the rKA group, with a statistically significant 5.2% intergroup difference, indicating faster postoperative recovery (p = 0.008). VAS pain scores decreased by 7% in the rKA group while increasing by 13% in the MA group (p < 0.001). At one-year follow-up, FJS-12 scores were significantly higher in the rKA group (94.8 ± 3.2 vs. 91.9 ± 2.2, p < 0.001). No significant differences were observed in KSS, OKS, or SF-36 score between groups. Conclusions: Restrictive kinematic alignment demonstrated superior early postoperative outcomes compared to mechanical alignment in RoTKA, with significantly reduced pain and improved ROM. While one-year functional outcomes were comparable between techniques, rKA may offer advantages in the immediate postoperative period, supporting its consideration as a viable alignment strategy in robotic-assisted knee arthroplasty. Full article
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17 pages, 2462 KB  
Article
Effect of Denture Adhesives on the Surface Roughness and Hardness of Denture Base Resins—A Preliminary Study
by Guilherme Bezerra Alves, Maria Margarida Sampaio-Fernandes, Carlos Fernandes, Francisco Góis, Bruno Graça, Estevam Bonfante and Maria Helena Figueiral
Appl. Sci. 2025, 15(19), 10749; https://doi.org/10.3390/app151910749 - 6 Oct 2025
Abstract
This study aimed to evaluate the impact of different adhesive solutions on the surface roughness and hardness of denture base materials. Twenty specimens (20 × 20 × 5 mm) were produced for each material group: heat-cured ProBase Hot®, 3D-printed NextDent Denture [...] Read more.
This study aimed to evaluate the impact of different adhesive solutions on the surface roughness and hardness of denture base materials. Twenty specimens (20 × 20 × 5 mm) were produced for each material group: heat-cured ProBase Hot®, 3D-printed NextDent Denture 3D+®, and PMMA-milled Exaktus®. They were then divided into five solution subgroups (n = 4): control (T0), distilled water, Corega PowerMax®, Elgydium Fix®, and Kukident Pro Ultimate®. Specimens were immersed in the solution at 37 °C daily for 28 days, simulating continuous use. Profilometry and Shore D hardness tests were performed at baseline and after 28 days of the immersion protocol. Data analysis was done using IBM SPSS Statistics 30.0, considering a confidence level of 0.05. At baseline, the materials differed in surface roughness and Shore D hardness, with the 3D-printed group showing the highest median values for the Rz parameter (p = 0.023) and the lowest for hardness (p = 0.023). Elgydium Fix had a significant effect on the heat-cured resin, with increased Rz and decreased hardness. Kukident caused higher roughness and lower hardness in the 3D-printed and milled resins (not significant). Corega showed minor effects in all tested materials. In conclusion, the denture base material and the adhesive formulation influence the physical and mechanical properties of denture base resins. Full article
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30 pages, 7188 KB  
Article
Performance Study and Implementation of Accurate Solar PV Power Prediction Methods for the Nagréongo Power Plant in Burkina Faso
by Sami Florent Palm, Aboubakar Gomna, Sani Moussa Kadri, Dominique Bonkoungou, Adélaïde Lareba Ouedraogo, Yrébégnan Moussa Soro and Marie Sawadogo
Energies 2025, 18(19), 5285; https://doi.org/10.3390/en18195285 (registering DOI) - 6 Oct 2025
Abstract
This study aimed to implement an effective power prediction method to support the optimal management of the 30 MW Nagréongo solar photovoltaic (PV) plant in Burkina Faso. Initially, the performance of the PV plant was assessed by an external consultant based on data [...] Read more.
This study aimed to implement an effective power prediction method to support the optimal management of the 30 MW Nagréongo solar photovoltaic (PV) plant in Burkina Faso. Initially, the performance of the PV plant was assessed by an external consultant based on data recorded in 2023 and 2024, revealing efficiency with a performance ratio (PR) of 73.73% in 2023, which improved to 77.43% in 2024. To forecast the plant’s power output, several deep learning models—namely LSTM, a GRU, LSTM-GRU, and an RNN—were applied using historical power data recorded at five-minute intervals during the 2024 periods of January–February; March–April; and July–August. All the deep learning models achieved accurate short-term forecasting for the 30 MW Nagréongo PV plant, with the seasonal performance shaped by the Sahelian weather regimes. The GRU performed best during the dry season (nRMSE ≈ 4%) and LSTM excelled in the hot months (nRMSE ≈ 2%), while the hybrid LSTM-GRU model proved most robust under rainy-season variability. Overall, the forecasting errors remained within 2–5% of plant capacity, demonstrating the suitability of these architectures for grid integration and operational planning in Sahel PV systems. Full article
(This article belongs to the Section A2: Solar Energy and Photovoltaic Systems)
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11 pages, 216 KB  
Article
Management of Normothermic Regional Perfusion Performance in Uncontrolled Versus Controlled Donation After Circulatory Death: A Multi-Center Investigation
by Chiara Lazzeri, Davide Ghinolfi, Manuela Bonizzoli, Daniele Cultrera, Paolo Lo Pane, Arianna Trizzino, Arianna Precisi o Procissi, Giuseppe Feltrin and Adriano Peris
J. Clin. Med. 2025, 14(19), 7053; https://doi.org/10.3390/jcm14197053 (registering DOI) - 6 Oct 2025
Abstract
Introduction: Controlled (c-) and uncontrolled (u-) DCDs are two entirely different types of donors, mainly because the duration of ischemic and reperfusion injury differs between them. We hypothesized that normothermic regional perfusion (NRP) management and performance (as indicated by the dynamic changes in [...] Read more.
Introduction: Controlled (c-) and uncontrolled (u-) DCDs are two entirely different types of donors, mainly because the duration of ischemic and reperfusion injury differs between them. We hypothesized that normothermic regional perfusion (NRP) management and performance (as indicated by the dynamic changes in blood flow and lactate) might be different in uDCDs and in cDCDs. Methods: We assessed 99 DCD donors that were consecutively evaluated by the Tuscany Regional Transplant Center from 2020 to 2024 (multi-center investigation), focusing on the comparison between NRP performance and management in uDCDs (n = 44) vs. cDCDs (n = 45). Results: NRP duration was significantly higher in uDCDs compared to cDCDs (p = 0.001). During NRP, we observed no changes in lactate values in uDCDs and cDCDs, a significant increase in transaminases, and a progressive reduction in NRP blood flow rates despite the administration of more fluids. Throughout the entire NRP duration, pH values were significantly lower and glucose levels were higher in uDCDs compared to cDCDs, even though a higher dosage of bicarbonate and insulin units were administered in uDCDs. Conclusions: In our series, we documented that NRP performance and management differed in uDCDs compared to cDCDs. This phenomenon may be mainly related to the different duration of the ischemic injury between these two types of donors. During NRP, uncontrolled DCDs showed a more severe metabolic derangement, which was only partially reversable by a more aggressive treatment (higher fluid volumes, insulin and bicarbonate dosages). Our results strongly suggest that there is likely space for optimization of NRP management in DCDs. Further research should address this issue, considering the disparity between the supply of organs and increasing transplantation needs. Full article
(This article belongs to the Section Intensive Care)
17 pages, 2525 KB  
Article
Dry Reforming of Methane Using Gd-promoted Ni/SBA-16 Catalyst: Structure, Activity and Process Optimization with Response Surface Methodology
by Salma A. Al-Zahrani, Mohammed F. Alotibi, Ahmed I. Osman, Ahmed A. Bhran, Maha Awjan Alreshidi, Ahmed Al Otaibi, Hessah Difallah A. Al-Enazy, Nuha Othman S. Alsaif and Ahmed S. Al-Fatesh
Nanomaterials 2025, 15(19), 1527; https://doi.org/10.3390/nano15191527 - 6 Oct 2025
Abstract
This work examines the effect of gadolinium (Gd) promotion on nickel-based SBA-16 catalysts for the dry reforming of methane (DRM), with the goal of improving syngas production by optimizing catalyst composition and operating conditions. Catalysts with varying Gd loadings (0.5–3 wt.%) were synthesised [...] Read more.
This work examines the effect of gadolinium (Gd) promotion on nickel-based SBA-16 catalysts for the dry reforming of methane (DRM), with the goal of improving syngas production by optimizing catalyst composition and operating conditions. Catalysts with varying Gd loadings (0.5–3 wt.%) were synthesised using co-impregnation. XRD, N2 physisorption, FTIR, XPS, and H2-TPR–CO2-TPD–H2-TPR were used to examine the structural features, textural properties, surface composition, and redox behaviour of the catalysts. XPS indicated formation of enhanced metal–support interactions, while initial and post-treatment H2–TPR analyses showed that moderate Gd loadings (1–2 wt.%) maintained a balanced distribution of reducible Ni species. The catalysts were tested for DRM performance at 800 °C and a gas hourly space velocity (GHSV) of 42,000 mL g−1 h−1. 1–2 wt.% Gd-promoted catalysts achieved the highest H2 (~67%) and CO yield (~76%). Response surface methodology (RSM) was used to identify optimal reaction conditions for maximum H2 yield. RSM predicted 848.9 °C temperature, 31,283 mL g−1 h−1 GHSV, and a CH4/CO2 ratio of 0.61 as optimal, predicting a H2 yield of 96.64%, which closely matched the experimental value of H2 yield (96.66%). The 5Ni–2Gd/SBA-16 catalyst exhibited minimal coke deposition, primarily of a graphitic character, as evidenced by TGA–DSC and Raman analyses. These results demonstrate the synergy between catalyst design and process optimization in maximizing DRM efficiency. Full article
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17 pages, 1814 KB  
Article
Right Ventricular Myocardial Metabolism and Cardiorespiratory Testing in Patients with Idiopathic Pulmonary Arterial Hypertension
by Natalia Goncharova, Aelita Berezina, Daria Ryzhkova, Irina Zlobina, Kirill Lapshin, Anton Ryzhkov, Aryana Malanova, Elizaveta Korobchenko-Andreeva and Olga Moiseeva
Diagnostics 2025, 15(19), 2523; https://doi.org/10.3390/diagnostics15192523 - 6 Oct 2025
Abstract
Background: Non-invasive diagnostic tools for the assessment of pulmonary arterial hypertension (PAH) are currently being intensively studied. Positron emission tomography (PET)/computed tomography (CT) with [18F]-fluorodeoxyglucose ([18F]-FDG) and [13N]-ammonia is the gold standard for assessing myocardial metabolism and perfusion. The relationship between right ventricle [...] Read more.
Background: Non-invasive diagnostic tools for the assessment of pulmonary arterial hypertension (PAH) are currently being intensively studied. Positron emission tomography (PET)/computed tomography (CT) with [18F]-fluorodeoxyglucose ([18F]-FDG) and [13N]-ammonia is the gold standard for assessing myocardial metabolism and perfusion. The relationship between right ventricle (RV) myocardial metabolism and perfusion and cardiopulmonary exercise testing (CPET) has not been studied. Objective: to evaluate correlations between the CPET parameters and RV perfusion and metabolism in IPAH patients. Methods: The study comprised 34 IPAH patients (34.2 ± 8.9 years, 4 males, 6 prevalent). Myocardial metabolism and perfusion were assessed using PET/CT with [18F]-FDG and [13N]-ammonia, respectively. CPET, cardiac MRI and invasive hemodynamics were also evaluated. Results: Significant negative correlations were registered between [18F]-FDG and [13N]-ammonia uptake by the RV (SUVmax RV/LV) and the oxygen consumption, oxygen pulse and positive correlation with the ratio of minute ventilation to carbon dioxide production. The low-risk IPAH patients significantly differed from the intermediate-to-high-risk group in CPET indices and in SUVmax RV/LV metabolism and SUVmax RV/LV perfusion parameters. No reliable differences in CPET indices and [18F]-FDG and [13N]-ammonia uptake by the RV were registered between intermediate- and high-risk patients. Conclusions: CPET is a reliable non-invasive diagnostic tool that could distinguish low-risk young IPAH patients without comorbidities from those at intermediate-to-high risk. Significant correlations between CPET parameters and RV myocardial metabolism and perfusion indices, MRI, and invasive hemodynamics confirm the high diagnostic value for CPET. Full article
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13 pages, 2298 KB  
Article
Dense Calcification of the Common Femoral Artery Is Protective Against In-Stent Restenosis
by Camil-Cassien Bamdé, Yann Goueffic, Jean-Michel Davaine, Alain Lalande, Charles Guenancia and Eric Steinmetz
J. Clin. Med. 2025, 14(19), 7052; https://doi.org/10.3390/jcm14197052 (registering DOI) - 6 Oct 2025
Abstract
Background: Vascular calcification has been highlighted as a prognostic factor for perioperative thrombosis but a protective factor for late restenosis in lower limb peripheral artery disease (LLPAD). The aim of this study was to investigate the association between calcification and twelve-month primary patency [...] Read more.
Background: Vascular calcification has been highlighted as a prognostic factor for perioperative thrombosis but a protective factor for late restenosis in lower limb peripheral artery disease (LLPAD). The aim of this study was to investigate the association between calcification and twelve-month primary patency in patients with stenting of the common femoral artery (CFA) and its bifurcation for atheromatous stenosis. Materials/Methods: This single-center retrospective study analyzed consecutive limbs (n = 90) that underwent CFA stenting for symptomatic lesions between January 2018 and January 2023. Calcification was assessed using dedicated computed tomography angiography analysis software (EndoSize; Therenva), with blinded evaluation of volume (mm3) and density (Hounsfield Units) across three anatomically distinct zones: proximal CFA (Zone 1); distal CFA (Zone 2); and bifurcation segments (Zone 3). The primary endpoint was twelve-month primary patency, defined as a peak systolic velocity ratio (PSVR) < 2.4 on duplex ultrasound without target lesion revascularization. Secondary endpoints included predictors of restenosis using multivariable logistic regression. Results: Ninety cases of CFA stenting for LLPAD (lower limb peripheral artery disease) were analyzed. A total of 78.9% of CFA lesions were treated for claudication and 21.1% for critical limb-threatening ischemia (CLTI). Lesions were distributed as Azema types I (1%), II (43%), and III (56%). At twelve-month follow-up, primary patency (PSVR < 2.4) was achieved in 77.4% of limbs. Patent CFA stenting demonstrated significantly higher median calcification density in Zone 2 compared to those with restenosis (1122 [IQR: 903–1248] vs. 858 [788–987] HU; p = 0.006; q = 0.021 after false discovery rate correction). ROC curve analysis identified a density threshold of 800 HU with a 76% reduction in restenosis risk (OR 0.24; 95% CI: 0.08–0.72; p = 0.011). Bootstrap validation (1000 replications) confirmed threshold stability at 821 HU (95% CI: 656–990 HU). Conclusions: In this exploratory study, dense calcification (≥800 HU) in the distal CFA appears to be protective against twelve-month restenosis following stenting. These findings suggest that calcification density may serve as a valuable predictor for patient selection and procedural planning in CFA interventions. Full article
(This article belongs to the Section Cardiovascular Medicine)
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