Search Results (626)

Red palm oil (RPO) is a rich source of bioactive compounds, including carotenoids, tocopherols, tocotrienols, and polyphenols, which contribute to its potent antioxidant and anti-inflammatory properties. This review summarizes the current understanding of RPO composition, bioactivity, and potential applications in health and cosmetics. Current preclinical and small-scale clinical studies suggest that RPO bioactives can mitigate oxidative stress, modulate inflammatory pathways, and improve skin barrier function. Strategies to enhance stability and bioavailability, such as microencapsulation and formulation into emulsions or liposomes, are also discussed. The manuscript highlights the potential of RPO as a natural functional ingredient in dietary, nutraceutical, and cosmetic products. Comprehensive evaluation of these bioactive compounds provides insights for future research and practical applications in promoting human health. Full article

The utilization of post-production and post-processing by-products aligns with current trends in sustainable fruit industry practices. Recovering valuable nutrients from such materials holds significant potential for the food, nutraceutical, pharmaceutical, and cosmetic sectors. Among these, cornelian cherry (Cornus mas L.) seeds represent a promising source of functional ingredients, particularly due to their oil’s rich nutritional and phytochemical profile. The seeds, accounting for approximately 9–10% of the fruit mass, yield an oil characterized by high levels of polyunsaturated fatty acids—mainly linoleic acid (≈67.5%) and oleic acid (≈20%)—alongside palmitic (≈5.8%) and stearic acids (≈2.1%). Linolenic acid content, however, shows notable variability (1.4–14.7%), influencing the oil’s omega-6/omega-3 ratio, which generally remains below 5:1. Cornelian cherry seed oil stands out among other stone fruit oils (e.g., rosehip, apricot, peach, cherry, plum) for its favorable fatty acid composition and absence of cyanogenic glycosides, making it safe for human consumption. Beyond its nutritional value, this oil exhibits biological activity and health-promoting potential, suggesting wide applicability in functional foods and nutraceutical formulations. Despite progress in characterizing seed composition—including proteins, lipids, carbohydrates, minerals, and tannins—knowledge gaps persist regarding the transfer of these compounds into the oil, particularly under cold-pressing conditions. Future studies should focus on optimizing extraction processes, assessing thermal treatment effects, and clarifying the variability of linolenic acid. Such research will support the sustainable exploitation of cornelian cherry by-products and the industrial-scale development of this high-value oil. Full article

Pitaya peels are an underutilized by-product rich in antioxidant phenolics and betalains but highly unstable under environmental and gastrointestinal conditions. This study aimed to produce bioactive-rich particles with improved bioaccessibility by applying ultrasound-assisted extraction (UAE) followed by freeze-drying encapsulation with maltodextrin (MD) and gum Arabic (GA) at different ratios (MD:GA 100:0, 75:25, 50:50, 25:75, 0:100, w/w). A rotatable central composite design (pH 4–8, time 10–50 min) was applied to optimize UAE conditions. Optimum conditions (pH 6.2, 35 min) yielded a pitaya peel extract with TPC 166.85 mg GAE·100 g⁻¹ DW. Although the betalain model was not significant, contents ranged from 30.20 to 51.99 mg·100 g⁻¹ DW across treatments. Particles produced with GA only (G100) had the highest phenolic (316.33 mg GAE∙100 g⁻¹ DW) and betalain (17.58 mg·100 g⁻¹ DW) contents, high solubility (~80%), low hygroscopicity (~11 g H₂O·100 g⁻¹ DW), and amorphous morphology. G100 showed enhanced in vitro bioaccessibility of betalains and phenolics compared to the non-encapsulated extract (p < 0.05). Betalain bioaccessibility decreased in the intestinal phase (~55%), consistent with pH-driven degradation, while phenolic bioaccessibility was higher (~140%). Overall, gum Arabic proved to be an effective carrier for stabilizing pitaya peel bioactives, supporting their valorization into functional food and nutraceutical ingredients. Full article

Bioactive peptides (BAPs) from dairy products have garnered increasing attention as natural agents with health-promoting properties, including antihypertensive, antioxidant, antimicrobial, immunomodulatory, opioid, and antidiabetic activities. This systematic review synthesizes research published between 2014 and 2024, retrieved from Scopus and PubMed, and selected according to PRISMA guidelines. A total of 192 studies met the inclusion criteria, collectively reporting over 3200 distinct peptides, with antihypertensive sequences, predominantly angiotensin-converting enzyme (ACE) inhibitors, constituting the largest category (n = 1237). β-casein was the principal precursor across bioactivities, followed by αs1-casein, β-lactoglobulin, and α-lactalbumin. Peptides were primarily produced via enzymatic hydrolysis, microbial fermentation, and gastrointestinal digestion, with peptide profiles influenced by the type of milk, microbial strains, and processing conditions. While cow’s milk remained the dominant source, investigations into goat, sheep, camel, buffalo, and donkey milk revealed species-specific biopeptides. Recent advances in proteomics have enhanced peptide identification and bioactivity prediction, enabling the discovery of novel sequences. These findings underscore the significant potential of dairy-derived BAPs as functional food components and nutraceutical ingredients, while highlighting the need for further in vivo validation, bioavailability studies, and broader exploration of underrepresented milk sources. Full article

Legumes are recognized for their high nutritional value, affordability, wide availability, and multiple health benefits. In Paraguay, nearly 200 varieties of creole beans are preserved. Despite their cultural and agronomic importance, there is little scientific information on their chemical composition in the country. This gap limits their potential use in food security policies and their development as nutraceutical ingredients for the food industry. This study aimed to generate scientific data on the nutritional profile, including macronutrients and macro- and microelements, of three creole bean varieties: Japanese cream, San Francisco, and Speckled. Macronutrient composition and macro- and microelements determinations were performed following AOAC (2000) methodology, while carbohydrate content was determined using the anthrone method of Clegg. The results revealed moisture contents between (10.1–11.4) g/100 g. Carbohydrate content was highest in San Francisco (69.9 g/100 g). This variety also showed superior protein (29.4 g/100 g) and lipid levels (1.83 g/100 g) compared to the others. Regarding minerals (macro and microelements), Speckled presented the highest iron (8.32 mg/100 g) and calcium (80.9 mg/100 g) values, whereas San Francisco contained higher levels of zinc (2.88 mg/100 g) and magnesium (219 mg/100 g). Copper (0.648–0.723 mg/100 g) and manganese (1.93–2.26 mg/100 g) values were consistent across the three varieties. The preliminary study will serve as a basis for providing initial information with a prospect of encouraging its use in new food applications, developing products with nutraceutical properties, promoting technological innovation, and developing healthy products. Full article

In recent years, edible flowers have gained increasing attention as unconventional foods, primarily due to their richness in bioactive compounds. Within this context, Clitoria ternatea L. (Fabaceae), commonly known as butterfly pea, stands out not only for its remarkable biological properties but also for its intense blue pigmentation. This review aims to provide a comprehensive overview of the plant’s potential in the food industry, highlighting its bioactive compounds, technological applications, and associated health benefits. Recent studies have demonstrated its antioxidant, antidiabetic, anti-obesity, hepatoprotective, and anticancer activities, as well as its use as a natural colorant, functional ingredient, active packaging component, and in nutraceutical and cosmetic formulations. Despite these promising findings, most available evidence comes from preclinical studies, with limited clinical validation to date. Therefore, further human studies are needed to confirm the efficacy and safety of the reported beneficial effects. Altogether, C. ternatea represents a promising natural resource for developing functional foods that meet the growing clean-label demand, fostering the incorporation of sustainable and natural ingredients. Full article

Natural deep eutectic solvents (NaDESs) have emerged as green and sustainable alternative solvents for extracting valuable bioactive compounds from agro-industrial by-products. NaDESs are stable, soluble, and biodegradable with low melting points and a wide range of applications. These characteristics align closely with the principles of green chemistry, making NaDESs promising for use in the food industry. Recent studies demonstrate that NaDESs can effectively extract proteins, polysaccharides, polyphenols, carotenoids, alkaloids, and other bioactives from sources such as vegetable waste, cereal by-products, and fruit pomace, often performing better than traditional solvents such as methanol and ethanol. The bioactive components of these extracts may exhibit antioxidant, anti-inflammatory, antihypertensive, anticancer, or antimicrobial activity and can be used as functional ingredients, nutraceuticals, or preservatives. Furthermore, NaDES-derived extracts have been shown to have hypoglycemic effects by inhibiting enzymes involved in the metabolism of carbohydrates and reducing oxidative stress. As a result, they may find use as functional food ingredients in diabetes management. This review presents the recent research on the extraction of bioactive compounds from agro-industrial by-products using NaDESs and an evaluation of their antidiabetic potential. Full article

Lactic acid fermentation is an effective strategy for food preservation and functional enhancement. This study evaluated the fermentation of Hericium erinaceus by-products using Lactoplantibacillus plantarum and Lactocaseibacillus rhamnosus, assessing microbial stability, physicochemical parameters, phenolic content, antioxidant activity, rheology, and biogenic amine formation over 240 h. Lp. plantarum promoted rapid acidification, reducing the pH from 6.0 to 4.65 within 72 h, while Ls. rhamnosus reached its lowest value of 3.8 at 144 h. Both strains effectively inhibited spoilage organisms: Lp. plantarum suppressed yeasts, molds, and Pseudomonas spp. by 144 h, whereas the control reached >6.0 log CFU/g of Pseudomonas at 240 h. Fermentation altered the texture, with the storage modulus (G′) decreasing from ~17 kPa to <3 kPa. Functional enrichment was also observed, with total phenolic content increasing from 32 to 48 mg GAE/100 g and antioxidant activity (DPPH) reaching 2562 µmol TE/100 g compared with 1954 µmol TE/100 g in the control. Importantly, cadaverine accumulated to 70.3 mg/kg in the control but remained below 15 mg/kg in inoculated samples, while spermidine was consistently higher in Lp. plantarum-treated mushrooms (~45 mg/kg). These results demonstrate that lactic acid fermentation can transform perishable H. erinaceus by-products into safe, stable, and bioactive ingredients, supporting their application in functional foods, nutraceuticals, and clean-label products while contributing to circular bioeconomy goals. Full article

Background/Objectives: Houttuynia cordata Thunb., a widely consumed vegetable and traditional food in Asia, possesses significant nutritional value. However, the impact of geographical origin on its nutritional metabolite composition, crucial for food quality and functionality, remains unclear. Methods: Here, we conducted a comprehensive metabolomic analysis of H. cordata cultivated across six major Chinese regions (Yunnan, Guangxi, Guizhou, Sichuan, Chongqing, Hubei) using UPLC-MS/MS. Results and Conclusions: We identified 496 nutritional metabolites, predominantly amino acids and derivatives (53.23%). Key bioactive carbohydrates, maltotriose and maltitol, exhibited distinct geographical accumulation patterns: maltotriose was significantly enriched in Yunnan (YN), while maltitol accumulated predominantly in Sichuan (SC). Integrated transcriptomic analysis linked this variation to the differential expression of starch metabolism genes (GBE1/glgB, α-amylases, β-amylases). Bioinformatic evaluation suggested potential health-related functionalities associated with these metabolites. These findings provide critical insights into the geographical determinants of H. cordata nutritional quality and functional properties. They offer a scientific foundation for optimizing cultivation practices based on regional advantages and developing H. cordata as a region-specific, high-value vegetable and functional food ingredient targeting distinct nutraceutical applications. Full article

The growing need for sustainable protein and functional food ingredients has made edible insects stand out as a flexible source of bioactives. Black Soldier Fly larva (BSFL) bioactives, such as chitooligosaccharides (COSs) and peptides, present potential benefits for gut health; nevertheless, their molecular pathways, clinical validation, and commercial scalability have yet to be thoroughly investigated. This study systematically analyzes current progress in BSFL bioactive extraction and characterization, emphasizing enzymatic and thermal processing, controlled enzyme development, and integrated supercritical fluid enzymatic pipelines. We assess preclinical and animal research that illustrates prebiotic modulation of Bifidobacterium, Lactobacillus, and Faecalibacterium populations; antimicrobial peptide-mediated immune signaling; and antioxidant activity. Multi-omics frameworks that connect the microbial metabolism of COS to gut health help us understand how these processes function. A comparison of the regulatory environments for food and feed applications in the EU, North America, and Asia shows that there are gaps in human safety trials, harmonized standards, and techno-economic assessments. Finally, we suggest some next steps: randomized controlled human trials in groups with irritable bowel syndrome (IBS) and metabolic syndrome; standardized data integration pipelines for multi-omics; and life cycle and cost–benefit analyses of modular, vertically integrated BSFL biorefineries with AI-driven reactors, digital twins, and blockchain traceability. Addressing these issues will hasten the conversion of BSFL bioactives into safe, effective, and sustainable functional meals and nutraceuticals. Full article

The revalorization of agro-industrial by-products is a key strategy for promoting sustainability and the circular economy. This study assessed the photoprotective potential of underutilized carob (Ceratonia siliqua L.) fractions, including unripe and mature pods, leaves, and seed tissues, through chemical characterization, in vitro assays, and in vivo validation. Extracts showed high polyphenol contents (up to 4.8 g GAE/100 g) and strong antioxidant activity (up to 45 g TE/100 g). Photoprotective properties were confirmed by a solar protection factor of up to 17 and erythema transmission values of 3–6, indicating efficient UV absorption and anti-inflammatory potential, which together support overall skin protection. To validate these effects under physiological conditions, Caenorhabditis elegans was used as an in vivo model under three exposure modalities: complete exposure (contact and ingestion), the barrier effect (UV shielding by the medium), and the physiological effect (systemic protection after ingestion). Seed episperm and unripe pods showed the highest efficacy. Notably, complete exposure reduced UV-induced lethality from 98% to below 50%, mainly due to the barrier effect. This is the first report demonstrating the photoprotective activity of carob by-products in C. elegans, supporting their potential as natural ingredients for cosmetic and nutraceutical applications, and contributing to the sustainable revalorization of local agricultural residues. Full article

Oxidative stress-induced mitochondrial dysfunction and apoptosis are critical factors in the pathogenesis of neurodegenerative diseases. This study investigated the synergistic neuroprotective effects of anthocyanin-enriched Morus alba L. extract combined with vitamin C (MAC) against hydrogen peroxide (H₂O₂)-induced oxidative stress in SH-SY5Y neuronal cells. Exposure to H₂O₂ triggered excessive reactive oxygen species (ROS) production and apoptosis, whereas treatment with MAC markedly alleviated these effects. Biochemical analyses revealed that MAC significantly reduced malondialdehyde (MDA) and enhanced the activities of antioxidant enzymes, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px), thereby contributing to improved redox balance. Furthermore, MAC modulated apoptosis-related signaling by upregulating extracellular signal-regulated kinase (ERK), cAMP response element-binding protein (CREB), and the anti-apoptotic protein B-cell lymphoma 2 (Bcl-2), while downregulating the pro-apoptotic protein Bcl-2-associated X (BAX) and cleaved caspase-3. Collectively, these findings demonstrate that MAC acts synergistically as a promising nutraceutical ingredient, supporting the development of functional foods for the prevention or mitigation of oxidative stress-related neurodegenerative disorders. Full article

Marine macroalgae (seaweed) are a rich source of bioactive polysaccharides such as agar, carrageenan, and alginate. These three compounds are classified as food additive ingredients, widely used as gelling, thickening, stabilizing, and emulsifying agents in the food, nutraceutical, pharmaceutical, and cosmetic industries. However, the growing concern for a safer world has sparked renewed interest in their safety evaluation. Unlike synthetic compounds with specified structures, seaweed polysaccharides exhibit substantial structural heterogeneity due to variations in species, habitat, and processing, affecting bioactivity, digestibility, and interactions within the gastrointestinal tract. Although the safety of these compounds is generally accepted, there are still significant gaps in our understanding of their physicochemical behaviour. This highlights the need to develop a standardized digestion model to ensure their safety and evaluate their potential long-term health effects. Most of these compounds are only partially absorbed in the upper gastrointestinal tract, where they are fermented into metabolites with varying health effects. The safety of carrageenan, in particular, remains a subject of debate due to ambiguous results reported by various researchers’ groups. This review highlights the importance of adopting standardized digestion assays, integrated analytical tools, and multidisciplinary approaches. These are crucial for thoroughly evaluating the molecular integrity, metabolism, and biological impact of seaweed polysaccharides, which will ultimately support evidence-based regulatory frameworks and ensure their safe use in human nutrition. This critical analysis focuses on food safety and security, with a methodology that can be applied to other foods or compounds. Full article

Chlorogenic acid (CGA), an ester of caffeic and quinic acids, is a dietary polyphenol abundant in coffee, tea, fruits, vegetables, and medicinal plants, with 5-O-caffeoylquinic acid (5-CQA) as its predominant isomer. This review aims to summarize current knowledge on the biological activities, mechanisms of action, and potential therapeutic applications of CGA in the prevention and management of metabolic disorders. CGA demonstrates a broad spectrum of biological activities relevant to human health. Its mechanisms of action involve modulation of oxidative stress and key cellular signaling pathways, as well as regulation of metabolic processes, contributing to improved insulin sensitivity, lipid balance, and overall energy homeostasis. These properties make CGA a promising agent against metabolic syndrome (type 2 diabetes, obesity, hypertension, and dyslipidemia) which is a major global health challenge. Despite its health benefits, CGA’s oral bioavailability remains limited, prompting research into optimized extraction methods, novel formulations, and structural modifications. Current evidence supports its safety even at high doses, reinforcing its potential as a nutraceutical, functional food ingredient, and adjunctive therapeutic compound in chronic disease management. Full article

Quince (Cydonia oblonga Mill.) processing generates peel and core by-product fractions that are underexploited resources with untapped potential for valorization in sustainable food systems. In this study, ultrasound-assisted extraction was performed using several choline chloride-based natural deep eutectic solvents (NADES, six formulations with distinct hydrogen-bond donors) and compared with 70% (v/v) ethanol. Extracts were analyzed for total phenolic content, antioxidant capacity, and individual phenolic compounds by LC-MS/MS, and their bioaccessibility was determined through a standardized in vitro digestion model. Organic acid-based NADES, particularly ChCl:MA (2:1) and ChCl:LA (1:1), yielded significantly higher phenolic contents from the peel than ethanol (up to ~45% increase, p < 0.05), and ChCl:MA maintained superior antioxidant capacity after digestion. In the core fraction, glucose- and glycerol-based NADES promoted the release of bound phenolics, resulting in bioaccessibility values exceeding 100%, indicating the release of previously bound phenolics under digestive conditions. The present study provides novel insights into the effects of NADES on both extraction efficiency and digestibility of quince by-products. These findings highlight quince peel and core as promising raw materials for developing functional food and nutraceutical ingredients, thereby offering a feasible strategy for upcycling fruit-processing residues into health-promoting applications. Full article