Search Results (6,720)

This review addresses the nutritional composition, health benefits, and claim conditions of aquaculture fish, focusing on gilthead sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax). Both species provide high-quality proteins, essential amino acids, and favorable lipid profiles, particularly long-chain omega-3 fatty acids, alongside minerals such as phosphorus and selenium, which meet EU criteria for several authorized nutrition and health claims. Evidence demonstrates that regular consumption supports cardiovascular, cognitive, and visual health, reduces inflammation, and contributes to better pregnancy and early childhood outcomes. Consumer skepticism toward aquaculture persists, often driven by perceptions of reduced nutritional quality, despite evidence that farmed fish provide nutritionally valuable proteins and beneficial lipids. Nevertheless, both species consistently meet the requirements for multiple nutrition and health claims, particularly those related to protein, omega-3 fatty acids, and selected minerals, allowing their effective use in labeling and consumer communication. Clear, evidence-based labeling of such claims is crucial to enhance acceptance and promote farmed fish as safe, sustainable, and health-promoting dietary choices. Full article

Botanical extracts have emerged as promising biostimulants in agricultural systems because of their ability to modulate key metabolic and redox processes in crops, thereby increasing stress tolerance and productivity. This review synthesizes current knowledge on how botanical extracts influence plant metabolism and redox homeostasis, with a particular emphasis on their role in adaptive cellular responses. Evidence indicates that these extracts can increase antioxidant enzyme activity, regulate reactive oxygen species (ROS) signaling, and promote the accumulation of bioactive metabolites associated with improved stress tolerance and enhanced growth. This review also examines how agronomic practices, including nutritional management, water availability, light regimes, and preharvest biostimulant applications, together with emerging biotechnological approaches, can be strategically employed to optimize the bioactive composition and efficacy of botanical extracts. By integrating recent advances in metabolomics and transcriptomics, the manuscript highlights the biochemical and molecular reprogramming triggered by botanical extracts. It identifies key challenges, including variability in extract composition, lack of standardization, and context-dependent responses. Finally, future research directions are outlined, emphasizing the need for mechanistic understanding, quantitative evaluation of plant responses, and the development of standardized frameworks to support the sustainable application of botanical extracts in agriculture. Full article

Feed nutrition and rise in antibiotic resistance are growing global challenges in aquaculture, with Aeromonas hydrophila causing significant losses in the carp family. This 60-day study evaluated the potential of combining herbal seed extract (Achyranthes aspera and Ricinus communis) with inactivated vaccine (A. hydrophila) to enhance growth and immunity in Labeo rohita. A total of 540 fish were randomly assigned in six groups (T0-untreated control, T1-A. aspera seed extract (ASE), T2-R. communis seed extract (RSE), T3-vaccinated control, T4-vaccine plus ASE, T5-vaccine plus RSE). Results revealed that herbal–vaccine combinations, particularly T4, showed highest growth performance (p < 0.05). Furthermore, this group showed improved metabolic profiles, indicated by reduced serum cholesterol, triglycerides, and glucose, along with significantly elevated albumin and globulin concentrations. In terms of immune response, neutrophil counts were significantly higher in T4 and T5. Crucially, following a challenge with A. hydrophila, survival rates were higher in T4:90% and T5:85% compared with positive control’s meager 20% survival. Post-challenge hematology confirmed that groups T4 and T5 maintained an enhanced immune status. These findings suggest that combining medicinal plants extract with vaccine effectively enhances growth, immunity, and disease resistance in L. rohita, presenting an environment friendly alternative to traditional antibiotics in aquaculture. Full article

This study optimized stabilizer type and concentration, and screened natural antioxidant combinations to enhance the stability of a protein beverage fortified with vitamins A, D₂, and D₃. Three stabilizers—carrageenan, sodium carboxymethyl cellulose (Na-CMC), and microcrystalline cellulose (MCC)—were evaluated at 0.15–0.45% (w/v) during accelerated storage at 45 °C for 21 days. Stability was assessed using Turbiscan analysis, pH, particle size, Zeta potential, and color. MCC at 0.35% demonstrated the best stabilization, with minimal changes in Turbiscan Stability Index, particle size, and Zeta potential. Five natural antioxidants—dl-α-tocopherol, vitamin C, epigallocatechin gallate (EGCG), tea polyphenols (TP), and pyrroloquinoline quinone (PQQ)—were screened for vitamin protection using HPLC. Although vitamin C exhibited the highest in vitro DPPH radical scavenging activity (IC₅₀ = 3.44 μg/mL), TP and EGCG provided superior protection of vitamins in the emulsion system. A synergistic antioxidant blend of EGCG, TP, and dl-α-tocopherol in a 4:4:2 mass ratio was identified as optimal, significantly prolonging vitamin retention over 21 days and yielding the longest predicted shelf-life (>84 days at 25 °C). These findings provide a practical formulation strategy for enhancing the physical and nutritional stability of functional protein beverages. Full article

Common wheat (Triticum aestivum ssp. vulgare) is one of the three major cereal crops cultivated worldwide and plays a key role in ensuring food safety. Adequate nitrogen supply is a key factor affecting the yield and functional properties of the grain of common wheat. Improving the efficiency of soil nitrogen use can be achieved through the application of appropriate mineral fertilizers and proper variety selection. The aim of this study was to determine the effect of residual nitrogen (N_res) remaining after maize cultivation on the functional properties of winter and spring wheat grain. The results of the present study clearly indicate that appropriate selection of the maize hybrid (preceding crop) and nitrogen fertilization strategy (residual nitrogen, N_res) can significantly enhance the antioxidant potential of grain in both forms of wheat (winter and spring). At the same time, our results highlight the practical importance of agronomic practices in improving the functional value of grain, both in terms of nutritional quality and health-promoting potential. Total polyphenol content in grain was stable, while antioxidant activity (ABTS⁺, DPPH) depended on genotype × fertilization interaction, particularly in winter wheat. These changes likely result from differences in polyphenol profile and the proportion of other antioxidants. Appropriate cultivar selection and nitrogen fertilization can enhance the antioxidant potential of wheat. No significant effect of either the preceding crop (maize) or its cultivar, or the form of nitrogen fertilizer, was found on the amino acid and total polyphenol content in winter and spring wheat grain. Population growth and the need to ensure adequate food supply highlight the importance of improving nitrogen management efficiency in agriculture by accounting for the amount and quality of residual soil nitrogen after the preceding crop. Full article

Providing dietary feedback is important for promoting healthy behaviors in weight management, but the rapid development of obesity and the shortage of medical nutrition human resources have limited this health service. The rise of large language models (LLMs) offers the possibility of using artificial intelligence (AI) to simulate the behavior of human dietitians. However, existing studies have only explored LLM performance when generating answers to common nutrition-related questions; the use of LLMs to generate situation-adapted dietary feedback in practical weight management scenarios still needs further research. In this study, we collected dietary records and dietary feedback from primary dietitians through an mHealth weight management application. We conducted topic modeling to generalize how dietitians deliver nutrition guidance in real-world dietary feedback scenarios. Combining the in-context learning capability of LLMs with real-world data, we proposed a synthetic data generation approach (HDI-SDG) and trained an LLM for dietary feedback with the synthetic data (LLMDF-EXP). Experiments on automatic and manual evaluation of LLMDF-EXP and an LLM trained directly with the real-world data as well as generalized LLMs illustrated that LLMDF-EXP performed most similarly to human experts. Notably, there were no significant differences from human experts in terms of professionalism (p-value = 0.510) and usefulness (p-value = 0.498). The study highlights that integrating LLMs with real-world data in health management processes can enhance the situational adaptability of LLMs in practical health management environment applications. Full article

The growing global population and increasing pressure on conventional food systems have intensified the search for sustainable and nutrient-rich protein sources. Blue foods derived from marine and freshwater organisms offer significant nutritional advantages and lower environmental footprints compared with many terrestrial animal proteins. However, challenges related to resource sustainability, processing, preservation, and product traceability limit their full potential. This review provides a broad overview of emerging technologies shaping the future of blue food systems, covering innovative production strategies, advanced processing techniques, and omics-based analytical approaches. Key developments in cellular aquaculture and cellular mariculture are discussed as promising alternatives to traditional fisheries and aquaculture, enabling the production of blue food through controlled cell cultivation. Additionally, alternative protein platforms including plant-based, fermentation-derived, and cultivated blue food analogues are assessed for their potential to enhance sustainability and diversify aquatic protein sources. Advanced structuring technologies such as extrusion, electrospinning, wet spinning, and 3D printing are highlighted for their roles in developing blue food analogues with improved texture and sensory attributes. Furthermore, non-thermal preservation techniques, including cold plasma (CP), high-pressure processing (HPP), pulsed electric fields (PEFs), and ultraviolet-based treatments, are reviewed for their effectiveness in improving microbial safety and extending shelf life while maintaining nutritional quality. The integration of omics technologies (proteomics, metabolomics, and lipidomics) provides deeper molecular insights into product quality, authenticity, and traceability within blue food supply chains. Collectively, these interdisciplinary advancements demonstrate strong potential to transform blue food production into a more resilient, sustainable, and technology-driven sector. Future progress will depend on overcoming challenges related to scalability, regulatory frameworks, and consumer acceptance to enable the successful commercialization of next-generation blue food products. Full article

The rice–wheat rotation is a predominant cropping pattern in China, frequently challenged by pests such as aphids in wheat, and Chilo suppressalis and Cnaphalocrocis medinalis in rice. This study investigates the potential of two common landscape plants, Photinia × fraseri and Pittosporum tobira, as functional plants for conserving natural enemies across crop cycles. Arthropod communities were systematically monitored using Malaise traps during the wheat, wheat–rice transition, and rice seasons from 2023 to 2024. Results revealed that both species successfully conserved a diverse natural enemy community, though their structural differentiation was strongly driven by seasonal variation, as confirmed by Heatmap and principal component analysis (PCA) (P. × fraseri: PC1 = 46.3%, PC2 = 23%; P. tobira: PC1 = 40.2%, PC2 = 25%). During the wheat season, both plants synergistically supported rich functional guilds, including predatory guilds (e.g., Episyrphus balteatus, Gnathonarium dentatum, and Harmonia axyridis) and parasitic guilds (e.g., Microplitis tuberculifer and Cotesia spp.). Notably, during the critical wheat-to-rice transition, these shrubs functioned as “habitat anchors,” where P. × fraseri demonstrated superior retention capacity for functional groups like Aphidius gifuensis, mitigating post-harvest habitat fragmentation. During the rice season, distinct functional complementarity emerged: P. × fraseri appeared to function as a habitat-type plant, potentially providing stable shelter for predatory groups (e.g., spiders and lady beetles), while P. tobira appeared to act as a resource-type plant, potentially attracting a significant rebound of parasitoids (e.g., Xanthopimpla flavolineata) in August. This mid-summer rebound on P. tobira was primarily attributed to its dense evergreen foliage providing a microclimatic refuge, rather than an active flowering resource. Analysis of shared dominant taxa (H. axyridis, Cotesia spp., and E. balteatus) showed highly significant seasonal fluctuations, with peak conservation during the wheat season. This study confirms that P. × fraseri and P. tobira have cross-cycle potential as a “natural enemy bank” in rice–wheat rotation agricultural systems. Their synergistic effects—integrating stable structural shelter with seasonal nutritional subsidies—support the conservation of diverse natural enemy communities throughout the annual crop cycle and significantly enhance the sustained pest control capacity of farmland ecosystems, identifying them as exemplary functional plants for ecological engineering in rice–wheat landscapes and providing a foundation for future studies on biological control efficacy. Full article

Tomato (Solanum lycopersicum L.) is a globally important vegetable, prized for its nutritional value and antioxidant content. Given the increasing demand for foods with health-promoting properties and the need for sustainable production practices, this study evaluated the impact of different growth substrates combined with plant growth-promoting rhizobacteria (PGPR) inoculation on the yield and nutraceutical quality of greenhouse tomatoes grown in a semi-hydroponic system. ‘Nereida’ variety saladette tomato plants were either inoculated with a single PGPR consortium (1 × 10⁸ CFU mL⁻¹) or uninoculated. Three substrates were used: a chemical fertilization control and a sand-vermicompost mixture with two inherent levels of phosphorus (253 and 442 ppm). The chemically fertilized substrate without inoculation served as the control treatment. The results indicated that the chemically fertilized substrate presented a significantly higher yield per square meter (p < 0.05), reaching values of 5.20 ± 0.70 kg m⁻² and 4.83 ± 0.35 kg m⁻² in the control treatment. However, fruits grown in the vermicompost-based substrate with higher phosphorus content (442 ppm) and PGPR inoculation exhibited significantly greater antioxidant capacity (54.16 µmol TE g⁻¹ FW) and higher concentrations of vitamin C (14.03 mg·100 g⁻¹ FW), lycopene (47.68 mg·100 g⁻¹ FW), flavonoids, carotenoids, and glutathione. This represented an increase of 28–45% in bioactive compounds including lycopene, vitamin C, flavonoids, carotenoids, and glutathione compared to the chemical control. While the interaction between substrate and inoculation was significant only for soluble solids, both factors independently and additively contributed to the enhancement of nutraceutical parameters. These findings suggest that the use of vermicompost-based substrates, particularly those with higher phosphorus content, in combination with PGPR inoculation, is a promising strategy to enhance the accumulation of health-promoting bioactive compounds in tomato fruits, despite a trade-off in total yield. Full article

This study investigated whether these contrasting environments are associated with the development of coherent, organism-wide phenotypic–physiological syndromes reflecting a fundamental life-history trade-off. A controlled 60-day trial was conducted comparing crayfish from a high-input pond system (fed to satiation) and a low-input rice paddy system (primarily natural diet). Pond crayfish were fed a commercial formulated feed twice daily to satiation (approximately 3% of biomass per day). Rice paddy crayfish primarily consumed natural food webs and received a once-daily supplement of 30% of the pond feed amount (approximately 0.9% of biomass per day). Results revealed two distinct syndromes. Pond-cultured crayfish exhibited a growth-oriented syndrome: they were 33% heavier with a compact, volumetrically enhanced body shape, coupled with elevated lipid-anabolic enzyme activities (lipase and ACC), higher hemolymph protein and cholesterol concentrations, but also significantly increased levels of a stress-related endocrine factor (cortisol-like immunoreactivity) and oxidative damage (MDA). Conversely, rice paddy-cultured crayfish displayed a stress-resistance-oriented syndrome: a streamlined, deeper-abdomen morphology, fortified antioxidant (SOD, CAT, and GST) and immune (lysozyme and phenoloxidase) capacities, enhanced carbohydrate digestion (amylase), and lower systemic stress. Transcriptomic analysis showed that the hepatopancreas of paddy-cultured crayfish was enriched in pathways related to lipid metabolism, detoxification, and endocrine regulation, notably with upregulation of the SULT1E1 gene. Our findings demonstrate that the distinct environmental and nutritional conditions of each aquaculture system are linked to specific multi-level adaptation syndromes, presenting a clear trade-off between rapid biomass production and systemic stress resistance (within the 60-day trial period), providing a mechanistic basis for optimizing sustainable practices: integrated systems enhance stock robustness, while intensive systems require strategies to mitigate physiological load. Full article

The ripe fruits of blackcurrant (Ribes nigrum L.) are rich in vitamin C, anthocyanins, and flavonoids. Besides being consumed fresh, the fruits can be processed into fruit juices, jams, wines, and other products, exhibiting considerable economic and nutritional value. Flavonoids are a class of important plant secondary metabolites with antioxidant, anti-inflammatory, and anti-cancer properties. Although previous studies have confirmed the involvement of multiple structural genes and transcription factors in flavonoid biosynthesis, the specific role of the dihydroflavonol 4-reductase (DFR) gene in regulating flavonoid accumulation during fruit development of blackcurrant remains to be clearly elucidated. In this study, we identified an RnDFR gene located in the nucleus and cytoplasm, which has the same expression trend as flavonoid content in fruit development stages. Overexpression of RnDFR improved the flavonoid accumulation and upregulated the expression levels of related structural genes (4CL, CHS, LDOX, ANR, and UFGT) in tomato. Transiently overexpressing RnDFR in blackcurrant fruit also increased the content of flavonoids and DFR enzyme activity, whereas silencing RnDFR resulted in the opposite effect. In addition, overexpression of RnDFR in tomato seedlings improved cold and drought tolerance by increasing flavonoid accumulation, reducing membrane lipid peroxidation damage and enhancing the activities of antioxidant enzymes. This study systematically reveals the key role of RnDFR in flavonoid biosynthesis and the enhancement of cold and drought tolerance, and offers an important theoretical basis for future efforts to optimize flavonoid content in blackcurrant and improve fruit nutritional quality. Full article

The rapid accumulation of fruit and vegetable waste and oyster shell residues presents increasing environmental challenges, particularly in regions with intensive agricultural and aquaculture production. This study evaluated the use of oyster shell powder as a calcium-based buffering additive to stabilize acidic fruit and vegetable waste during rearing of black soldier fly larvae (BSFL, Hermetia illucens). Five substrates containing 0, 2, 4, 6, and 8% oyster shell powder (fresh weight basis) were prepared and used for larval rearing under controlled conditions for 12 days. Substrate pH dynamics, larval growth performance, substrate utilization efficiency, and nutritional composition of larval biomass were assessed. Oyster shell supplementation significantly increased substrate pH in a dose-dependent manner, shifting the substrate from strongly acidic conditions toward the range favorable for larval development. Moderate supplementation levels (2–4%) resulted in the highest larval biomass, survival rate, dry matter reduction, and bioconversion efficiency, whereas excessive supplementation reduced performance. Protein content increased at moderate supplementation levels, while lipid content decreased with higher oyster shell inclusion. Calcium concentration in larval biomass increased proportionally with supplementation, whereas essential amino acid composition remained stable. These results demonstrate that oyster shell powder can be used as an effective buffering material to improve the stability of acidic organic waste substrates and enhance BSFL-based bioconversion. The combined utilization of fruit and vegetable waste and oyster shell residues represents a practical approach for integrated waste valorization and supports the development of circular bioeconomy strategies for sustainable protein production. Full article

Interactions between alcohol and nutrition play an important role in the development and progression of alcohol-associated liver disease (ALD). Although dietary cholesterol was shown to exacerbate fatty liver and liver injury in alcohol-fed mice, findings regarding the combined effect of dietary cholesterol and heavy alcohol drinking on cholesterol homeostasis remain controversial. Ezetimibe has been widely used as a cholesterol-lowering drug in hypercholesterolemic subjects. It is not fully understood whether ezetimibe blunts the adverse effect of cholesterol on lipid and biliary bile acid metabolism in alcohol-exposed mice. In the current study, wild-type mice were subjected to NIAAA alcohol feeding model. Dietary cholesterol (0.2%, w/v) and ezetimibe (0.001%, w/v) were added to the liquid diets. Cholesterol and triglyceride contents in the liver and circulation were determined. Biliary bile acid composition, as well as hepatic and circulating inflammatory markers were analyzed. We found that ezetimibe protected mice from the synergistic effects of dietary cholesterol and alcohol on hepatic triglyceride accumulation, which was accompanied by enhanced expression of genes involved in hepatic beta oxidation. Dietary cholesterol caused great increases in liver cholesterol content and dramatic reductions in the expression of hepatic cholesterol biosynthetic genes in both control- and alcohol-fed mice. These changes were normalized by ezetimibe treatment. Ezetimibe attenuated dietary cholesterol-induced elevations in total biliary bile acids. Moreover, mice fed a diet containing both cholesterol and alcohol exhibited increased expression of monocyte chemoattractant protein 1 (Mcp1) and tumor necrosis factor alpha (Tnfα) in the distal small intestine. Collectively, our findings indicate that ezetimibe effectively mitigates the adverse effects of dietary cholesterol and alcohol consumption on hepatic lipid accumulation and liver injury. Full article

Background: The incidence and mortality rates of cancer among females aged 65 years or older in the Republic of Korea are increasing; however, the national cancer screening rate (50.4%) remains low. Therefore, this study aimed to identify predictors of participation in the National Cancer Screening Program (NCSP) among women aged 65 years or older using data from the 8th Korea National Health and Nutrition Examination Survey (KNHANES VIII, 2019–2021). Methods: This cross-sectional study utilized data from the 8th Korea National Health and Nutrition Examination Survey (KNHANES VIII, 2019–2021). Study variables were selected based on Andersen’s healthcare utilization model. Participation in the National Cancer Screening Program (NCSP) was defined as the dependent variable, and independent variables included predisposing, enabling, and need factors. Descriptive analyses were conducted to examine participants’ characteristics. Chi-square tests were used to assess differences in NCSP participation according to participant characteristics. Multivariable logistic regression analysis was conducted to identify factors associated with participation, with all independent variables simultaneously included in the model to adjust for potential confounding. A p-value of <0.05 was considered statistically significant. Results: A total of 2105 women aged 65 years or older were included in the analysis. Of the 2105 women aged 65 years or older, 1429 (67.9%) reported participation in cancer screening within the past two years. NCSP participation was significantly associated with being married (OR = 1.540; 95% CI: 1.263–1.879), being a middle school (OR = 1.357; 95% CI: 1.022–1.801) or college graduate or higher (OR = 2.012; 95% CI: 1.199–3.378), having private insurance (OR = 1.930; 95% CI: 1.573–2.368), average subjective health (OR = 1.332; 95% CI: 1.004–1.766), dyslipidemia (OR = 1.347; 95% CI: 1.110–1.636), and physical activity participation (OR = 1.252; 95% CI: 1.029–1.524). In contrast, urban residence, income level, being employed, medical coverage type, hypertension, diabetes, monthly drinking status and current smoking status were not statistically significantly correlated with NCSP participation. Conclusions: These findings highlight the need for tailored outreach strategies and health education programs targeting women aged 65 years and older to enhance participation in the NCSP and ultimately improve public health outcomes. Full article

Background/Objectives: Adequate nutrition in older adults is essential to maintaining health, functionality, and quality of life, particularly in long-term care hospitals (HACLEs). Previous studies suggest that dissatisfaction with hospital food is linked to longer stays, more complications, and negative perceptions of care. Given these concerns, this study aimed to assess patients’ experiences with hospital food over a 15-year period in a HACLE in Spain, identify key influencing factors, and validate an updated PREM (Patient Reported Experience Measure) tool for food services. Methods: A retrospective, observational, repeated cross-sectional study was conducted using annual PREM surveys administered between 2011 and 2025 to patients on oral diets. Psychometric validation of the updated 8-item version (2024) was conducted. Results: Out of 1618 surveys, 1540 were included in the final analysis. The updated PREM showed strong internal consistency (α = 0.85, ω = 0.87), a two-factor structure (food quality and service conditions), and adequate model fit. Perceptions worsened after a catering company change in 2022 but improved following the implementation of new food distribution carts in 2025. The PREM total score showed a strong positive association with the global satisfaction item, providing supportive evidence based on a closely related anchor measure (Spearman’s rho = 0.80, 95% CI 0.77–0.82; p < 0.001). Scores differed significantly by diet type: patients receiving a pureed diet reported the highest average satisfaction score, followed by those on a soft diet and a regular diet. The group on a soft diet excluding foods that pose a choking hazard had the lowest mean score. Conclusions: The validated PREM scale is a reliable tool to monitor patient experience with hospital food. It enables early detection of quality issues and supports targeted improvements. Routine use in long-term care settings may foster personalized, patient-centered nutrition strategies and enhance care quality. Full article