Search Results (272)

Climate change represents one of the most critical challenges, especially in the Mediterranean area. Using organic and localized fertilization could be an effective agroecological strategy to help mitigate the environmental impacts of climate change. Our study was carried out in an experimental field over a three-year crop succession including broccoli, sweet pepper and barley. A randomized complete block design was adopted, with two factors: (i) fertilization method (100% broadcast and 40% localized) and (ii) fertilizer type by testing: on-farm compost, two types of commercial compost and a mineral fertilizer. Environmental impacts per hectare and marketable yield were quantified using Life Cycle Assessment (LCA), considering abiotic depletion (AD), acidification (AA), eutrophication (EU), global warming potential (GWP), and photochemical oxidation (PO). The localized application of fertilizers achieved marketable yields comparable to the broadcast method, despite lower fertilizer inputs, suggesting an optimal nutrient-use efficiency. The LCA demonstrated that localized fertilization also enhanced environmental sustainability, decreasing Global Warming Potential (GWP) by 20% per hectare, compared to broadcast treatments. Moreover, considering only the fertilization phase, we observed a 59% reduction in GWP under the localized strategy. Even though localized fertilization emerges as an effective climate-smart strategy without compromising productivity, future research is recommended to assess its long-term impacts in site-specific conditions. Full article

On-farm welfare assessment of equines is a challenge given the large diversity of management practices, especially in terms of housing and activities. In our study, we tested time budget measures as a complementary tool to more conventional welfare indicators (e.g., stereotypic behaviours, ear position while foraging, neck shape). We observed 174 horses living in eight facilities (in their home environment) for which data on management practices and welfare were available. Time budget was assessed using the scan sampling method (1 min scan sampling over 30 min; 33 scans), while welfare assessment was based on classical measures. The study was performed in two parts: The first part consisted of validating time budget as a correlate of welfare state, which was performed at the individual level and according to the context of observation. In the second part, the data were analysed at the farm level by averaging data from all horses in the same facility. The results showed that the time spent feeding, in exploratory walking and observation behaviour were correlated with indicators of positive welfare and/or good practices in contrast to the time spent standing immobile resting or in fixed attention and/or in negative social interactions. Time budget varied greatly between facilities, reflecting welfare state and management (feeding, working) practices. This study shows that, provided that observations are made at different time periods when animals are calm, three sessions of 10 min. could give a good account of the local «atmosphere». These findings bring new insights into both methodological approaches and the significance of behaviours and, in particular, the importance of precisely defining and measuring the types of attention as a window into an animal’s internal state. Full article

Background: Antibiotic-free approaches to boar semen preservation are gaining importance to counter emerging antimicrobial resistance. Hypothermic storage at 5 °C, instead of the conventional 17 °C, is a promising strategy to eliminate antibiotics still commonly used in extenders. For practical adoption, the method must be simple and compatible with on-farm routines. Objective: To assess fertility when cooling was initiated on farm one day after delivery, and to evaluate the robustness of cold-stored semen to temporary warming and subsequent re-cooling, mimicking typical handling on insemination days. Methods: Individual ejaculates (n = 34) from six boars were extended in Androstar^® Premium either without antibiotics (5 °C) or with gentamicin (17 °C control). One day after collection, antibiotic-free doses were cooled on farm to 5 °C and used alongside controls in routine insemination of 270 sows. Sperm quality was evaluated by computer-assisted semen analysis and flow cytometry, and bacterial counts were monitored. In a separate test, cold-stored doses were exposed to 20 °C for 60 min and re-cooled to 5 °C. Results: Farrowing rates and litter sizes did not differ between groups (p > 0.05). In antibiotic-free samples after 120 h, bacterial counts were mostly not detectable or low (<10² CFU/mL). Sperm motility and plasma membrane integrity in cold-stored doses remained >80%, comparable to controls (p > 0.05). Temporary warming did not affect sperm quality or bacterial counts. Conclusions: Antibiotic-free semen storage at 5 °C is easy to implement in practice and maintains fertility under field conditions. Broader validation under routine conditions is encouraged in support of the One Health concept. Full article

Animal welfare monitoring is essential in pig production. On-farm animal welfare (AW) assessments may provide a comprehensive overview but are resource-intensive. Abattoir-based assessments allow pigs from multiple farms to be inspected in a single facility. However, data on the relationship between these assessments remain limited, especially for heavy pigs (160–170 kg). This study investigates these associations in Italian heavy pig production. At the abattoir, 18,333 pig carcasses from 185 batches across 86 farms were scored for tail, skin (cranial and caudal) and ear lesions. On-farm AW assessments (management, structures and animal-based measures) were obtained from the national surveillance system (ClassyFarm). Tail lesion scores were higher in pigs with intact tails, whereas ear scores showed the opposite trend, suggesting a substitution effect between tail and ear biting. This indicates that tail docking is insufficient to fully prevent abnormal behaviours. Higher skin and ear scores were associated with suboptimal management, but tail scores were not, likely due to the multifactorial nature of tail biting. Herd size had no significant effect on welfare indicators. These results highlight the complexity of assessing AW and the importance of combining abattoir and farm data to obtain a more integrated monitoring system. Full article

Accurate, non-contact monitoring of rumination behavior is essential for assessing dairy cow health and welfare, as well as for optimizing feeding strategies and herd management in modern precision livestock farming. However, practical deployment in commercial barns faces challenges such as occlusions, variable lighting, and dynamic cow movements. To address this, we developed a robust, automated vision-based framework for continuous rumination monitoring. The core of our system integrates an enhanced object detection algorithm with a robust tracking module, specifically improved to capture subtle behavioral features and maintain identity under complex conditions. Evaluated on a comprehensive dataset collected from commercial settings under various lighting and occlusion scenarios, our framework achieved high detection accuracy (mAP of 96.26%) and reliable tracking performance (multi-object tracking accuracy of 99.2%). This demonstrates its suitability for real-time, on-farm deployment. The study provides a practical, end-to-end solution for fine-grained behavioral analysis in complex environments, offering a tool that can enhance welfare assessment and support decision-making in dairy farm management. The methodological approach is also adaptable to other precision livestock monitoring tasks. Full article

Sheep welfare outcomes vary depending on production systems, breeds, and environmental conditions. This study examined the effects of extensive, semi-extensive, and semi-intensive sheep production systems on animal welfare in Serbia, using the AWIN Welfare Protocol to evaluate 30 farms. Welfare indicators were categorised into resource-based, management-based, and animal-based metrics. The results indicated that there was no significant difference in space allowance among the production systems (p > 0.05). This suggests that the space provided was adequate for semi-intensive farms and suitable for both semi-extensive and extensive farms. However, management practices showed significant variations (p < 0.05), indicating diverse impacts on sheep welfare. No ocular discharge or stereotypic behaviours were observed, while respiratory issues, social withdrawal, and excessive itching were found to have a very low prevalence across all farms. The primary welfare concern identified in the extensive farms was the use of painful mutilations. Semi-extensive and semi-intensive farms had significantly higher rates of tail docking (p < 0.05) and poorer fleece cleanliness. These findings highlight the necessity of addressing the root causes of poor welfare to improve sheep welfare standards. Therefore, achieving sustainable welfare outcomes requires an integrated approach that combines genetic suitability, adequate housing, and effective management practices. Full article

This pilot study aimed to evaluate the early postpartum dynamics of lactoferrin concentration in bovine colostrum and to investigate its relationship with rapid on-farm quality indicators during the first 12 h after calving. Colostrum samples were collected from six multiparous cows immediately after calving (0 h) and again 12 h later. Colostrum specific gravity and % Brix values were measured on-farm, and lactoferrin concentration was determined using LC–MS/MS analysis. Temporal changes were assessed using the Wilcoxon signed-rank test, and relationships between variables were evaluated using linear regression and Spearman correlation analysis. Lactoferrin concentration decreased significantly between 0 and 12 h after calving (median: 3.350 vs. 2.175 mg/mL; p = 0.031). In parallel, statistically significant decreases were observed in both colostrum specific gravity and % Brix values over the same period (p = 0.031 for both indicators). Linear regression analyses showed positive slopes between lactoferrin concentration and specific gravity and between lactoferrin concentration and % Brix at both 0 and 12 h after calving; however, these relationships did not reach statistical significance (p > 0.05 for all models), with explained variance ranging from R² = 0.156 to 0.409. Spearman correlation analysis also revealed moderate positive correlation coefficients (p > 0.05), although none of the correlations reached statistical significance. These results indicate a rapid decline in lactoferrin concentration during the first 12 h after calving, occurring in parallel with significant decreases in widely used on-farm colostrum quality indicators. Despite the lack of statistically significant associations, the observed positive relationships indicate that lactoferrin may represent an additional component of colostrum composition that is not directly reflected by refractometric and density-based indicators. Full article

Interannual variability in climatic conditions represents a major source of uncertainty in cool-climate viticulture, highlighting the need for cultivar-specific assessments of climate–quality relationships. A multi-year on-farm experiment with six monitoring sites has been conducted in vineyards representative of the Tokaj wine region to monitor and assess vintage effect. This study, as the first part of a broader research project evaluating must components, quantifies relationships between climatic indices and key yield- and sugar-related traits (berry weight, total soluble solids, and total dry extract) in Vitis vinifera L. cv. Furmint grown in the Tokaj wine region over three contrasting vintages. Thermal, radiative, and water-availability variables were calculated for discrete phenological phases and statistically analyzed to identify climatic predictors of berry growth and must composition. Berry weight exhibited pronounced vintage sensitivity, showing consistent associations with precipitation-related variables during early developmental stages. In contrast, total soluble solids and total dry extract displayed weaker and less consistent responses to interannual climatic variability. Several widely used heat-accumulation indices showed limited explanatory power, indicating a moderate climatic sensitivity of sugar-related traits in this cultivar. Overall, the results suggest that early-season climatic conditions exert a stronger influence on berry growth than late-season thermal extremes, while compositional parameters related to sugar accumulation remain comparatively stable. These findings highlight the need to incorporate cultivar-specific response functions into statistical models that assess projected climate-change effects on grape quality. Full article

Cenchrus fungigraminus (Juncao) is a high-yielding, fast-growing forage crop with considerable potential for livestock feed; however, optimizing its processing is essential for cost reduction and quality enhancement. This study comprised three components: (1) a comprehensive analysis of 25 on-farm silage samples from five locations in Southwest China using Grey Relational Analysis (GRA); (2) an assessment of the effects of three cutting heights (low: 100–150 cm; mid: 150–200 cm; high: 200–250 cm) on silage quality; and (3) a comparison of silage quality between natural fermentation and microbial inoculant treatments using mature Juncao (250–300 cm). The results showed that: (1) in the on-farm silage samples, carbon supplementation was significantly positively correlated with total digestible nutrients (TDN), relative feed value (RFV), ether extract (EE), and sensory evaluation (p < 0.05), and the GRA identified the top-ranked treatments, including J2, J3, J6, X6, and J5; (2) in the cutting height trials, fiber content increased significantly with cutting height (p < 0.05), while crude protein (CP) and TDN decreased significantly (p < 0.05). The 200–250 cm group exhibited optimal fermentation quality, characterized by the highest total volatile fatty acids (total VFA) and lactic acid concentrations, alongside the lowest pH and ammonia nitrogen/total nitrogen ratios (NH₃-N/TN); (3) in the inoculant comparison, the natural fermentation group demonstrated significantly higher degradation rates of acid detergent fiber (ADF), neutral detergent fiber (NDF), and acid detergent lignin (ADL) compared to the microbial inoculant group, while also maintaining a lower pH, higher total VFA and lactic acid. Consequently, for on-farm production, carbon supplementation is recommended to improve silage quality. Although cutting Juncao below 200 cm provides higher nutritional value, a height of 200–250 cm is advised to ensure optimal fermentation characteristics. Furthermore, natural fermentation proves superior to microbial inoculant treatment for mature Juncao. Together, these measures offer an effective strategy for producing high-quality Juncao silage. Full article

In Türkiye, where agriculture consumes 75% of available water and national irrigation efficiency is only 51.3%, irrigation modernization—the conversion of classical open-channel irrigation systems to pressurized pipe systems—presents a primary strategy to achieve significant water savings. This study provides a comprehensive economic assessment of the potential of this strategy. A twofold methodology was employed: first, a cost–benefit analysis (CBA) of the 36,108 ha Ivriz irrigation project, and second, a national model to simulate the economic impact of modernizing nation’s 4.9 million hectares currently irrigated by such classical systems. This approach directly addresses two gaps identified in the literature: the lack of comprehensive project-level economic assessments of full irrigation modernization in large-scale open-channel systems, and the absence of simulations quantifying the national-level economic potential of modernizing Türkiye’s classical irrigation infrastructure. The Ivriz case study reveals that project viability is entirely contingent on the on-farm efficiency achieved post-modernization. At 60% efficiency, water savings are insufficient to make the project economically feasible, whereas at 90% efficiency, substantial water savings render the project highly profitable. At the national level, the analysis indicates that the conserved water could be used to expand Türkiye’s irrigated area by 1.77–2.98 million hectares, generating an additional $3.47–$5.84 billion in annual agricultural income. The findings conclude that while modernization represents a powerful investment, its success requires a comprehensive policy framework that not only funds infrastructure conversion but also mandates integrated support programs to ensure farmers adopt the high-efficiency technologies needed to achieve these savings. Full article

Biosecurity plays a central role in preventing disease transmission in dairy production systems and animal welfare. However, quantitative data on biosecurity implementation in smallholder and medium-scale dairy farms remains inconsistent, especially in developing countries. This study provides a structured assessment of on-farm biosecurity practices in northern Kosovo using a standardized, risk-based scoring approach. A cross-sectional survey was conducted on 55 dairy farms using the unmodified Biocheck.UGent™ dairy questionnaire. External and internal biosecurity scores were calculated through predefined, weighted algorithms and analyzed using non-parametric descriptive statistics. Farm-level results were subsequently compared with international reference values derived from the Biocheck.UGent™ global database. The median biosecurity scores for Kosovo farms were 47.8% for external biosecurity and 29.0% for internal biosecurity, indicating uneven implementation with pronounced weaknesses in measures designed to limit within-herd transmission. The lowest-scoring domains were purchase and reproduction and feed and water within external biosecurity, and working organization and equipment, calf management, and calving management within internal biosecurity. In contrast, visitors and farmworkers, control of vermin and other animals among external measures, and adult cattle management among internal measures, showed relatively higher scores, although all remained below international reference levels. When compared with the global overall biosecurity reference median of 76.7% derived from the Biocheck.UGent™ database, the biosecurity performance of the surveyed dairy farms in Kosovo was substantially lower. This gap does not indicate a complete absence of biosecurity measures but rather an uneven application, with the most pronounced deficiency observed in routine practices that govern within-herd disease transmission. The use of a risk-based scoring system allowed these weaknesses to be identified in a structured manner and placed the Kosovo results within an international benchmarking framework. In this context, the approach functions as a practical diagnostic tool, enabling farmers and veterinarians to prioritize feasible, epidemiological-relevant improvements within small- and medium-scale dairy production settings. Full article

Avian reovirus (ARV) infections pose a significant and evolving threat to China’s poultry industry, the world’s largest. Diverse farming systems—ranging from modern intensive operations to traditional waterfowl-poultry polyculture—foster a unique ecological niche for ARV, defined by complex serotypic and genotypic diversity, marked regional variations, potential interspecies transmission between chickens and waterfowl, and recurrent co-infections. Collectively, these factors undermine the efficacy of conventional diagnostic approaches. This review systematically outlines the current epidemic landscape of ARV in China, highlighting the molecular characteristics of prevailing strains (particularly those from waterfowl) and their roles in diagnostic evasion. We critically assess the performance and limitations of existing diagnostic techniques (virus isolation, ELISA, PCR/qPCR) within the Chinese epidemiological setting. Furthermore, we discuss innovative technologies—including multiplex qPCR, CRISPR-Cas systems, and next-generation sequencing (NGS)—that offer potential for developing next-generation diagnostics tailored to China’s specific challenges. Finally, we propose future directions, with an emphasis on standardization, data sharing, and interdisciplinary collaboration to bridge the gap between cutting-edge innovation and on-farm application for precise ARV control. Full article

The intensification of commercial chicken production has increased antimicrobial use and manure generation, raising concerns about residues and resistant pathogens entering the environment. Use of raw chicken manure can introduce antimicrobial compounds and resistance determinants into agricultural soils. This study examined antimicrobial use and manure management practices among chicken farmers in Morogoro, Dar es Salaam, and Unguja, and identified key gaps in national regulatory frameworks and their on-farm implementation. A structured questionnaire was administered to 351 farmers to assess the types and usage of antimicrobials and manure handling practices. Farmers reported using fourteen antibiotic classes and four antiparasitic agents, with tetracycline being the most frequently used (54.1%). Most farmers in Unguja (97.7%), Dar es Salaam (87.3%), and Morogoro (70.9%) either apply manure as fertilizer, sell it, or both. A large proportion (93.2%) reported that they do not process manure before use or sale, mainly due to lack of technical knowledge (77.4%). Awareness of the health hazards posed by pathogens (43.3%) and drug residues (57.5%) is low. This study revealed critical gaps, including weak regulatory enforcement, inadequate surveillance systems, limited cross-sectoral integration, irrational antimicrobial use, and limited farmer awareness. Strengthening regulatory frameworks, improving farmer training, and promoting safer manure management methods are recommended to reduce the environmental dissemination of antimicrobial residues and resistance. Full article

Small ruminants, such as newborn lambs, rely on timely colostrum intake to acquire passive immunity through the absorption of immunoglobulin (Ig). Evaluating Ig transfer is important for ensuring lamb health and survival. However, current methods such as enzyme-linked immunosorbent assay (ELISA) and radial immunodiffusion (RID) are widely used but remain costly and require specialized facilities. The immunocrit assay has been proposed as a lower-cost alternative for evaluating serum Ig concentrations. This study aimed to evaluate the immunocrit method in lambs by comparing it with ELISA, RID, and total serum protein. Serum was collected from 135 Katahdin-Easycare lambs 24–36 h after birth. Samples were analyzed using sheep immunoglobulin G ELISA, Sheep immunoglobulin G RID, serum protein, and the immunocrit method. Pearson’s correlation was used to assess linear relationships between the methods, and Receiver Operating Characteristics (ROC) analysis was used to evaluate test accuracy, with RID as the gold standard (15 mg/mL cutoff). The immunocrit showed a high correlation with RID (r = 0.870), moderate correlation with serum protein (r = 0.725), and good correlation with ELISA (r = 0.607). The ROC analysis showed that the immunocrit had a sensitivity of 100% at a cutoff of 4.34%. These results indicate that the immunocrit method provides comparable accuracy to RID and serum protein, and could serve as a reliable, practical, and inexpensive tool for on-farm evaluation of passive transfer in Katahdin-Easycare crossbred lambs between 24 and 36 h after birth. Full article

Unmanned aerial vehicle (UAV)-based multispectral monitoring has become an increasingly important tool for assessing crop vigor and stress under commercial agricultural conditions. However, most UAV-based studies using the normalized difference vegetation index (NDVI) in citrus systems have focused on yield estimation, disease detection, or canopy characterization during active growth phases, while the immediate post-harvest recovery period remains poorly documented. In this study, UAV-derived NDVI products were used to evaluate the canopy response in a commercial ‘Washington Navel’ orange orchard located in La Yarada Los Palos district (Tacna, Peru) following harvest. The study specifically assessed the effect of an on-farm, residue-based organic biostimulant produced from local organic wastes within a circular economy framework. The results indicate that treated plots exhibited a faster and more pronounced recovery of canopy vigor compared to untreated controls during the early post-harvest period. By integrating high-resolution UAV-based multispectral monitoring with a residue-derived biostimulant strategy, this work advances current NDVI-based applications in citrus by shifting the analytical focus from productive stages to post-harvest physiological recovery. The proposed approach provides a scalable and non-invasive framework for evaluating post-harvest canopy dynamics under water-limited, hyper-arid conditions and highlights the potential of locally sourced biostimulants as complementary management tools in precision agriculture systems. Full article