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Keywords = pETDuet-2 × Udh

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11 pages, 3070 KB  
Communication
Biosynthesis of Glucaric Acid by Recombinant Strain of Escherichia coli Expressing Two Different Urinate Dehydrogenases
by Xinchao Yang, Linlin Niu, Chunjiang Ye, Yuanxiu Wang, Yuehui Liu, Fang Wang and Naxin Sun
Fermentation 2023, 9(8), 764; https://doi.org/10.3390/fermentation9080764 - 17 Aug 2023
Cited by 4 | Viewed by 4158
Abstract
D-glucaric acid is an important bio-based building block of polymers and is a high value-added chemical that can be used in a variety of applications. In the present study, the Udh target genes from Pseudomonas putida and Pseudomonas syringae were used together to [...] Read more.
D-glucaric acid is an important bio-based building block of polymers and is a high value-added chemical that can be used in a variety of applications. In the present study, the Udh target genes from Pseudomonas putida and Pseudomonas syringae were used together to construct the expression vector pETDuet-2 × Udh. The transformants of BL21 (DE3) with vector pETDuet-2 × Udh were applied to produce glucaric acid from glucuronic acid. After optimizing the induction conditions, the highest Udh expression was achieved when 0.4 mmol·L−1 isopropyl-β-d–thiogalactoside (IPTG) was added to the cell cultures at an OD600 value of 0.6 followed by culturing at 26 °C for 6 h. The production of glucaric acid substantially reached 5.24 ± 0.015 g·L−1 in fed-batch cultures in a 30 L tank. In the present study, a new system for glucaric acid production was established, which was more economic and friendly to the environment. Full article
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