Search Results (1,353)

Under physiological and pathological conditions, all cells release extracellular vesicles named exosomes, which act as transporters of lipidic, protein, and genetic material from parent to recipient cells. Neoplastic cells can secrete higher number of exosomes to exert pro-tumoral effects such as microenvironmental changes, disease progression, immunosuppression and drug-resistance. This holds true for both organ-specific cancers and hematologic malignancies. One of the most important components of exosomal cargo are microRNAs which can mediate all the abovementioned effects. More specifically, microRNAs are small non-coding RNAs, routinely detected through quantitative real-time PCR, which act as translational suppressors by regulating protein-coding genes. Considering their high stability in all body fluids and viability in circulation, research is currently focusing on this type of RNAs for the so called “liquid biopsy”, a non-invasive tool for disease diagnosis and longitudinal monitoring. However, several issues remain to be solved including the lack of standardized protocols for exosome isolation and miRNA detection. Starting with this premise, our review aims to provide a wide description of the known microRNA panels employed in the prominent hematological malignancies, which will hopefully redefine the approach to these very challenging diseases in the near future. Full article

Soil element background concentration is foundational data for environmental quality assessment, contamination diagnosis, and sustainable land management. However, existing investigation-based methods are time-consuming and inefficient. The machine learning (ML) method has demonstrated excellent performance in predicting soil heavy metal concentration. In this study, based on the nine environmental variables of soil formation from 210 soil monitoring points, including elevation, pH, organic matter, soil type, parent material, plant cover, land use type, topography, and soil texture, decision tree (DT), random forest (RF), extreme gradient boosting (XGB), and support vector machine (SVM) models were used to predict the eleven soil element background concentrations. Among them, SVM and RF models could be used for an effective prediction of the background concentration of all soil heavy metals. Compared with the XGBoost and DT, the SVM for all heavy metals except for cadmium (Cd) and manganese (Mn) performs best. Although the key factors affecting background concentrations vary among different soil elements, organic matter, soil type, and altitude, they play a crucial role in the accurate prediction of soil element background concentration. This study provides simple and efficient ML models for predicting soil element background concentration at the large regional scale. The results of this study can be utilized to distinguish natural geochemical processes from human-induced pollution. Full article

Emerging research evokes selection for various plant secondary compounds as a potential driver of ruminant diet selection, through animals’ evident ability to rectify deficiencies and even self-medicate. This idea was assessed by comparing physiological responses to vaccination challenges of animals fed diets of differing phytochemical composition. In the first of three separate trials, goats were placed in individual pens and fed one of three treatments in a completely randomized design. Treatments in Trial 1 consisted of redberry juniper (50 g) and shin oak (50 g). In Trial 2, goats were fed rations containing grape and blueberry pomace at an inclusion rate of 20%. In Trial 3, black Angus heifers were fed rations containing grape and blueberry pomace at an as-fed inclusion rate of 6%. Average daily gain, intake, and blood chemistry were assessed following vaccination health challenges. In Trial 1, goats fed shin oak had higher (p < 0.05) blood globulins. Trial 2 revealed no treatment group differences in average daily gain (ADG), intake, or blood parameters evaluated. In Trial 3, no difference occurred in blood parameters; however, intake following inoculation was significantly greater (p < 0.05) for heifers with grape/blueberry pomace included in their rations. In conclusion, phytochemicals, specifically condensed tannins, may have the ability to enhance immune response in ruminants, but further research is required, and these effects likely depend upon the source, structure, and dose of tannins or parent plant materials offered. Full article

Oxygen transport membranes (OTMs) have gained a lot of attention for their application in different innovative fields, but the development of new materials able to combine high oxygen permeability and good chemical stability is crucial to boost the exploitation of such membrane-based technologies. Perovskite oxides are widely studied as mixed ionic-electronic conductors for the realization of OTMs. In this article, we focus on _Sr1-xCa_xTi_0.8Fe_0.2O_3-ẟ (SCTF) perovskites and investigate the effect of Ca content on the A-site of the permeation properties, both in single-phase SCTF membranes and in dual-phase membranes obtained by combining SCTF and the ionic conductor Ce_0.8Gd_0.2O₂ (CGO). In single-phase samples, we observed that the substitution of 40% Ca preserves the permeation performances of the non-substituted SrTi_0.8Fe_0.2O_3−ẟ membrane while allowing for a substantial decrease in the sintering temperature, thus facilitating membrane manufacturing. In dual-phase membranes, the increase in the Ca content in the perovskite causes an increase in grain size. The permeation is, at least partially, controlled by the kinetics of the surface exchange reactions. This limitation can be overcome by the addition of an activation layer; however, the permeance of activated CGO-SCTF membranes still remains lower compared to the single-phase parent perovskitic membranes. Full article

In 2023, the authors conducted a qualitative study in five bilingual educational institutions (two general education schools and three kindergartens) in Tartu, Estonia, undergoing a transition to Estonian-medium education. The empirical material for this qualitative research was collected during ten discussion evenings with Russian L1 parents, with around 300 attendees. Given the emotional and political sensitivity of the topic, the discussions were documented through researchers’ handwritten field notes and subsequently reconstructed from these notes for thematic analysis following the principles of qualitative content analysis. This study aimed to map the concerns and fears of Russian L1 parents and to collaboratively explore possible solutions. The broader objective was to understand and interpret Russian-speaking parents’ attitudes toward the shift to Estonian-medium instruction. A further aim was to raise language awareness among parents and to help lay a more positive foundation for the transition process. The theoretical framework draws on the notion that parents’ language attitudes significantly influence their children’s perceptions of the value of the language being learned. Our results show that many Russian L1 parents in Tartu consider it important for both Estonian- and Russian-speaking children to study in a shared, Estonian-medium learning environment. At the same time, parents identified several key challenges, including concerns about a decline in education quality, increased academic pressure and stress for children learning in a non-native language, a lack of suitable learning materials, and parents’ limited ability to assist with homework due to their own insufficient proficiency in Estonian. Full article

Background: RMS is the most common soft tissue sarcoma in children. Pencil beam scanning proton therapy (PBS PT) enables highly conformal dose delivery with reduced exposure to surrounding healthy structures, making it particularly suited for RMS in critical anatomical regions. Long-term clinical outcome data for this new radiation technique are scarce. Purpose: This study reports long-term outcomes and quality of life after PBS PT in children and adolescents with rhabdomyosarcoma (RMS). Methods and Materials: We retrospectively reviewed 114 children and adolescents with RMS (mostly embryonal, n = 100; 87.7%) treated between 2000 and 2020. Their median age was 4.6 years (range, 0.3–18). All received systemic chemotherapy according to prospective protocols. The median total PT dose delivered was 52 Gy (RBE; range, 41.4–64.8). Results: After a median follow-up period of 7.1 years (range, 0.3 to 17 years), we observed 26 failures overall; 21 (80.8%) occurred in-field. The 5-year local control and overall survival were 81.2% and 81%, respectively. The composite endpoint (non-ocular grade ≥3 toxicity- and failure-free survival) counting the first occurrence of any failure (local or distant), death, or non-ocular CTCAE v5.0 grade ≥3 toxicity was 77.3% at 5 years. At the start of PT, parents and children reported a quality of life significantly worse than that of a German normative group, but during the follow-up period, their scores improved to normal values in nearly all domains within two years. Conclusions: Our two decades of experience with PBS PT provide data that reflect good local control rates and minimal late non-ocular grade 3 toxicity. We also show that quality of life returned to normal scores in nearly all domains within 2 years. Children and adolescents with RMS seem to benefit from PBS PT in terms of toxicity and quality of life, but further prospective, multi-institutional comparative trials are needed. Full article

As an interspecies hybrid inheriting genetic material from horse and donkey lineages, mules provide a unique model for studying allele-specific regulatory dynamics. Here, we isolated adult fibroblasts (AFs) and placental fibroblasts (PFs) from mule tissues and reprogrammed them into induced pluripotent stem cells (iPSCs). Intriguingly, placental fibroblast-derived iPSCs (mpiPSCs) exhibited reduced reprogramming efficiency compared to adult fibroblast-derived iPSCs (maiPSCs). Through allele-specific expression (ASE) analysis, we systematically dissected transcriptional biases in parental cell types and their reprogrammed counterparts, revealing conserved preferential expression of asinine alleles in core pluripotency regulators (e.g., POU5F1/OCT4, SOX2, NANOG) across both cell lineages. Strikingly, mpiPSCs displayed stronger asinine allele dominance than maiPSCs, suggesting tissue-specific parental genomic imprinting. Mechanistic exploration implicated PI3K-AKT signaling as a potential pathway mediating the reprogramming inefficiency in placental fibroblasts. By integrating transcriptomic profiling with ASE technology, this study uncovers allele selection hierarchies during somatic cell reprogramming in hybrids and establishes a framework for understanding how parental genomic conflicts shape pluripotency establishment. These findings advance interspecies iPSC research by delineating allele-specific regulatory networks and providing insights into the molecular constraints of hybrid cellular reprogramming. Full article

Sorghum is a crucial food crop, and utilizing heterosis is significant for yield enhancement. To classify heterotic groups in sorghum, 96 inbred lines (48 male sterile lines and 48 restoring lines) were previously analyzed using whole-genome resequencing (WGRS) technology, from which 9691 high-quality SNP markers were obtained. In this study, the materials were divided into two groups—Group I (36 lines; predominantly restoring lines) and Group II (60 lines; mainly male sterile lines)—according to their genetic distances, and 8 lines were selected from each group for incomplete diallel crosses, producing 64 hybrid combinations for analyzing ten agronomic traits and their relationship with heterosis and combining ability. Heterosis analysis revealed that yield-related traits (plant weight, grain yield, and single-spike grain weight) exhibited the strongest heterosis, followed by morphological and developmental traits. The general combining-ability variance exceeded the specific combining-ability variance for traits controlled by additive gene effects. The results demonstrate that WGRS technology effectively classifies heterotic groups in sorghum, providing scientific support for parent selection in hybrid breeding. While combining-ability analysis offers higher predictability for heterosis than molecular genetic distance, genetic distance remains valuable for predicting heterosis. Full article

Rural–urban student migration during the compulsory education stage is a transitional phenomenon in China’s socio-economic development and a crucial issue for achieving the goal of urban–rural integration. This paper, grounded in theoretical analysis, constructs a “willingness-capacity-behavior” framework. Based on field survey data from 916 rural households and in-depth interview materials from County D, Province X, China, this study employs a bivariate Probit model and qualitative analysis methods to explore the driving factors and formation mechanisms of rural households’ rural–urban student migration decisions. The results indicate that rural households’ decisions regarding rural–urban student migration are jointly influenced by migration willingness and migration capacity. Only households with both migration willingness and migration capacity can actualize migration behavior. Migration willingness is derived from a cost–benefit analysis and involves joint decision-making by both parents, significantly influenced by parental personal characteristics and the student’s individual characteristics. The intermediate barriers to rural–urban student migration require certain migration capacities to be overcome, which are mainly influenced by family resource endowment and parental personal characteristics. Full article

This study presents a novel environmentally-friendly process for the selective extraction and enrichment of DHA/EPA-containing phospholipids (PL-DHA/EPA) from krill oil. The methodology leverages differential crystallization behavior between phospholipids and triacylglycerols in ethanolic solutions, exploiting their distinct freezing point thresholds to achieve precise fractionation. Response surface methodology optimization identified optimal extraction parameters: liquid-to-material ratio of 6:1 (v/w), freezing temperature of −20 °C, freezing duration of 25 h, and rotary evaporation temperature of 45 °C, yielding a final product with 39.40% PL-DHA/EPA content. Principal component analysis revealed substantial overlap in confidence ellipses among extraction methodologies, indicating effective preservation of core phospholipid signatures from the parent krill oil while maintaining critical structural characteristics and molecular species distribution. Comprehensive analysis of phospholipid fractions and heatmap analysis revealed distinctive molecular profiles compared to conventional organic solvent extraction, with selective enrichment of EPA-containing phospholipids, particularly PC-EPA and PI-EPA species. The green extraction method demonstrated comparable oxidative stability to conventional approaches, with superior protection against secondary oxidation as evidenced by significantly lower anisidine values. This sustainable approach achieves effective phospholipid enrichment while substantially reducing environmental impact through elimination of halogenated solvents, addressing the critical need for environmentally conscious technologies in marine lipid processing with potential applications in nutraceutical and functional food industries. Full article

Polycyclic aromatic hydrocarbon (PAH) derivatives, specifically azaarenes and nitrated and oxygenated PAHs, are emerging contaminants of concern due to their increased toxicity and persistence compared to the parent PAHs. Despite their toxicity, their simultaneous analysis in complex matrices, such as in fumes emitted from bituminous mixtures, remains challenging due to limitations of conventional analytical techniques. To address this, an advanced methodology was developed using Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass Spectrometry (UHPLC-HRMS Orbitrap Eclipse) equipped with an APCI source for the simultaneous identification and quantification of 14 PAH derivatives. Chromatographic and ionization parameters were optimized to ensure maximum sensitivity and selectivity. Following ICH Q2(R2) guidelines, the method was validated, demonstrating excellent linearity (R² > 0.99), high mass accuracy (≤5 ppm), strong precision (<15%), and excellent sensitivity. Limits of detection (LODs) ranged from 0.1 µg L⁻¹ to 0.6 µg L⁻¹ and limits of quantification (LOQs) ranged from 0.26 µg L⁻¹ to 1.87 µg L⁻¹. The validated method was successfully applied to emissions from asphalt pavement materials collected on quartz filters under controlled conditions, enabling the identification and quantification of all 14 targeted compounds. These results confirm the method’s robustness and suitability for trace-level analysis of PAH derivatives in complex environmental matrices. Full article

Purple totomoxtle (maize husk) in native maize represents a phenotypic trait of cultural and agronomic significance within traditional Mesoamerican agroecosystems. This study evaluated the phenotypic expression of anthocyanins in vegetative and reproductive tissues of ten native maize genotypes, including inter-parental crosses derived from both pigmented and non-pigmented lines. Field trials were conducted under rainfed conditions in Chiapas, Mexico. Visual and quantitative assessments included pigmentation intensity, chlorophyll and carotenoid content, ear traits and appearance, grain health, and yield performance. Genotypes exhibiting the purple phenotype showed consistent pigment accumulation in stems, nodes, leaf sheaths, tassels, and bracts (totomoxtle), with statistically significant differences compared to non-pigmented controls. Anthocyanin content in totomoxtle increased by 30% during late developmental stages, whereas chlorophyll and carotenoid levels peaked during early vegetative growth. Pigmented materials displayed healthier grain, enhanced ear appearance, and higher yields, with the JCTM × LLMJ cross reaching 6.60 t ha⁻¹. These findings highlight the functional value of purple totomoxtle and its potential in agroecological programs aimed at resilience, genetic conservation, and integral resource utilization, providing useful criteria such as stable pigment expression and superior yield to guide sustainable reproduction strategies. Full article

Oat is a dual-purpose crop valued for both grain and forage. The stay-green (SG) trait, which delays leaf senescence and prolongs photosynthesis, has been shown to increase yield and quality in several crop species, yet its performance across diverse environments in oats remains underexplored. In this study, multi-location field trials were conducted in Ledu, Huangzhong and Haiyan, Qinghai Province, China, to comprehensively evaluate the performance of stay-green oat lines. The traits evaluated included grain yield components, nutritional quality, and seedling establishment traits. A TOPSIS (technique for order preference by similarity to an ideal solution) model, coefficient of variation (CV) and G × E (genotype × environment) visualization were used to assess adaptability, stability, and genotype × environment interactions. On average, the stay-green lines exhibited an 16.00% increase in plot yield and a 22.93% increase in thousand-grain weight compared to controls. Notable improvements were also observed in the starch (7.58% LN_SG in HZ and HY) and protein (3.58%, QY5_SG all the sites) contents, as well as multiple seedling establishment indices, with the seedling vigor indices increasing by more than 50%. Stability analysis further showed that the stay-green lines were stable in spike length, thousand-grain weight, water-soluble carbohydrates, and seed and seedling vigor. TOPSIS analysis identified ‘LN_SG’ as the top-performing and most adaptable genotype across all environments. Overall, stay-green oat lines demonstrated superior performance in grain yield, nutritional quality, and seedling establishment. These findings highlight their potential for field application and their value as parental materials in oat breeding programs enhancing environmental adaptability and stability. Full article

Introduction: Pain represents a significant threat to the physical and psychological well-being of children, negatively affecting their quality of life during hospitalization. Pain is considered the fifth vital sign and must be regularly assessed and managed, as also emphasized by the nursing code of ethics. The interdisciplinary approach to pediatric pain management includes both pharmacological treatments and non-pharmacological techniques (NPTs), taking into account the child’s age and specific needs. NPTs comprise a broad set of methods, ranging from simple to complex, that can be applied to children to help them manage pain. The main objective of this study was to explore and analyze which non-pharmacological methods are adopted by nurses in their clinical practice to relieve pain in school-aged children (6–12 years) undergoing surgery. Materials and Methods: This observational study involved nursing staff from pediatric wards in the Italian provinces of Ravenna, Forlì-Cesena, and Rimini, and used a validated online questionnaire. The study focused on school-aged children (6–12 years) who had undergone surgical procedures. The questionnaire included items on which NPTs nurses used to relieve pain in pediatric patients. Participants responded using a Likert scale from 1 (never) to 5 (always), and anonymity and voluntary participation were guaranteed. Data were collected between February and October 2024, involving the pediatric units of three hospitals in the provinces of Ravenna, Forlì-Cesena, and Rimini. Statistical analyses included t-tests, ANOVA, and Kruskal–Wallis tests to identify significant differences. Results: A total of 46 nurses completed the questionnaire. No significant differences were found between nurses’ backgrounds and the use of NPTs. Overall, nurses did report using NPTs, although there was limited use of such techniques in the preoperative phase. The study also highlighted a discrepancy in the information provided to children versus parents, with nurses tending to give more information to parents during the preoperative period. Notably, nurses who reported effective multidisciplinary collaboration were also those who better prepared children using NPTs. Conclusions: This study emphasizes the importance of NPTs in pediatric pain management and highlights the need to improve direct communication with children. Adopting an effective multidisciplinary approach is essential to ensuring a less traumatic surgical experience for young patients. Full article

Background: Maize is an important food crop in cold regions, especially in Northeast China. However, its short growth period and low-temperature stress pose challenges to the breeding of high-yield hybrids. With climate warming, the maize planting area continues to expand to high latitudes. Research on cold-region maize is of great significance to ensure food security and sustainable agricultural development. However, most of the current maize research is concentrated in temperate and tropical regions, and there are few studies on cold-region maize. Methods: Based on this, this study selected some representative cold-region maize materials and materials whose adaptability has not yet been verified, and used a semi-diallel hybrid design for hybridization to determine the general combining ability (GCA) and specific combining ability (SCA) to screen out excellent breeding materials suitable for cold regions. Field experiments were carried out under four different cold environments, and 55 hybrid progenies and their parents were evaluated. The double allele hybridization analysis based on the Griffing method 2 (model 1) showed that the specific combining ability (SCA) and general combining ability (GCA) effects of each trait were significant. Results: The GCA mean square of all traits except yield and number of grains per row was greater than the SCA mean square, indicating that additive gene effects were dominant and genetic improvement through selective breeding was feasible. Hayman plot analysis under four environments showed that yield, ear length, number of grains per row, water content, and plant height were mainly controlled by superdominant genes, while stem thickness, number of nodes, and ear position were controlled by some dominant genes. Conclusions: Parent P1 contained more recessive genes in yield traits, but more dominant genes in number of grains per row, number of nodes, and ear position; P3 contained more dominant genes in yield and water content, but more recessive genes in number of nodes and ear position; P7 contained more recessive genes in most traits; and P9 contained more dominant genes in most traits. P9 and P6 represent excellent parental germplasm, among which the hybrid combinations P1 × P9, P2 × P5, P3 × P10, P4 × P6, P5 × P8, P6 × P9, P7 × P10, and P8 × P10 all show hybrid vigor exceeding that of their parents and have high breeding value. Full article