Search Results (1,231)

Human engagement in extreme activities, from spaceflight to deep-sea diving and extreme sports, presents unique physiological challenges. Understanding the molecular mechanisms underlying adaptations to these demands is crucial for developing strategies to enhance human performance and resilience in such environments. This review integrates multi-omics data across a range of extreme phenotypes, including astronauts, scuba divers, acute alcohol consumers, long-haul flight passengers, bodybuilders, and simulation racers. We analyze current literature in genomic, transcriptomic, proteomic, metabolomic, and metagenomic studies to identify common and phenotype-specific adaptations, highlighting potential biomarkers and pathways associated with resilience in harsh conditions. This integrated approach offers insights into human adaptability and provides a foundation for developing personalized strategies to mitigate risks and enhance performance in extreme environments, with particular relevance to extended spaceflight. Full article

For monitoring animal adaptation when facing environmental challenges, and more specifically when addressing the impacts of global warming—particularly responses to heat stress and short-term fluctuations in osmotic regulations in the different organs influencing animal physiology—there is an increasing demand for digital tools to understand and monitor a range of biomarkers. Microneedle arrays (MNAs) have recently emerged as promising devices minimally invasively penetrating human skin to access dermal interstitial fluid (ISF) to monitor deviations in physiology and consequences on health. The ISF is a blood filtrate where the concentrations of ions, low molecular weight metabolites (<70 kDa), hormones, and drugs, often closely correlate with those in blood. However, anatomical skin differences between human and farm animals, especially large animals, as well as divergent tolerances of such devices among species with behavior specificities, motivate new MNA designs. We addressed technological challenges to design higher microneedles for farm animal (pigs and cattle) measurements. We designed microneedle arrays composed of 37 microneedles, each 2.8 mm in height, using dextran-methacrylate, a photo-crosslinked biocompatible biopolymer-based hydrogel. The arrays were characterized geometrically and mechanically. Their abilities to perforate pig and cow skin were demonstrated through histological analysis. The MNAs successfully absorbed approximately 10 µL of fluid within 3 h of application. Full article

Proper hydration is essential for maintaining homeostasis and the effective functioning of physiological systems, including the nervous and circulatory systems. During adolescence, a period characterized by rapid somatic growth, hormonal maturation, and increased physical and mental activity, the demand for water increases significantly. Hydration affects not only the health of young people, but also their cognitive abilities, concentration, mood, and general well-being. Despite clear recommendations from institutions such as EFSA and IOM regarding daily fluid intake, numerous studies indicate that a significant proportion of young people do not achieve the recommended level of hydration. The school environment is particularly worrying, as young people spend a significant part of their day there, and the availability of water, health knowledge, and social conditions may contribute to dehydration or promote unhealthy choices (e.g., sweetened drinks). The aim of this article is to review the current state of knowledge on the importance of hydration in school-age adolescents. The physiological basis of hydration, the impact of insufficient fluid intake on the functioning of the young body, current guidelines, as well as the results of selected epidemiological studies and obstacles to ensuring optimal hydration in the school environment are discussed. Full article

The accumulation of plastic waste has intensified the pursuit of biodegradable alternatives, yet standard methods such as CO₂ evolution, oxygen demand, and mass loss fail to fully capture microbial physiological responses during degradation. This study introduces a biochemical assay-based approach to quantify proteins, lipids, and carbohydrates in soil as indicators of microbial activity during polymer biodegradation. For microcrystalline cellulose (MCC), proteins, lipids, and carbohydrates increased by 2.09-, 6.47-, and 11.22-fold, respectively (all p-values < 0.001), closely aligning with CO₂ evolution trends. Non-biodegradable poly(vinyl chloride) (PVC) exhibited no significant changes. Synthesized poly(butylene glutarate) (PBG) also showed significant biomolecule accumulation (up to 2.70-fold) alongside CO₂ production. Biomolecule quantification complements CO₂-based methods by revealing microbial proliferation and metabolic activity that persist beyond the mineralization plateau, offering a more comprehensive assessment of biodegradability. Full article

Non-alcoholic fatty liver disease (NAFLD) has emerged as a significant metabolic disorder in modern poultry production, particularly affecting high-yielding laying hens. This condition compromises bird welfare, productivity, and economic sustainability within commercial farming systems. This narrative review provides a comprehensive overview of the underlying mechanisms through which hepatic lipid accumulation, metabolic dysfunctions, hormonal imbalances, genetic susceptibilities, and environmental stress contribute to the development of NAFLD. The multifactorial nature of NAFLD is explored through a critical assessment of the literature, highlighting the influence of diet composition, management practices, and physiological demands associated with intensive egg production. Emphasis is placed on recent advancements in nutritional modulation, selective breeding, and housing improvements aimed at prevention and mitigation of NAFLD. Furthermore, the review identifies key research gaps, including limited understanding of epigenetic influences and the long-term efficacy of intervention strategies. An integrative framework is advocated, synergizing genetics, nutrition, and environmental optimization to effectively address the complexity of NAFLD in poultry and supports the development of resilient production systems. The insights presented aims to inform both future research and practical applications for enhancing poultry health and performance. Full article

Heparin, an essential plasma-derived therapy, acts as a naturally occurring anticoagulant and is essential in various physiological processes. Due to its complex structure, repeating units of sulfated glycosaminoglycan, it attracts attention in the field of commercial pharmaceuticals. In recent decades, significant advancements have been made in the development of economical adsorbents designed especially for the extraction of heparin from the intestinal mucosa of pigs, as evidenced by investments from various pharmaceutical industries. This requirement arises from the demand for efficient, scalable extraction methods for natural sources. In this study, we investigated the application of beta zeolites to increase the recovery of heparin from real porcine mucosa samples, emphasizing materials with greater adsorption surfaces, higher thermal stability, and increased porosity. According to our research, the zeolite CP814E’s macropores and huge surface area allow it to adsorb up to 20.6 mg·g⁻¹ (39%) of heparin from actual mucosa samples. We also investigated the adsorbent’s surface conditions, which are essential for efficient heparin recovery, and adjusted temperature and pH to enhance heparin uptake. These findings demonstrate that zeolite-based adsorbents can enhance the extraction of heparin effectively for use in medicinal applications. Full article

Background: Hand surgery is increasingly transitioning from hospital operating theatres to outpatient settings, requiring anaesthetic methods that are efficient, cost-effective, and patient-centred. Traditional anaesthesia, such as general anaesthesia, poses challenges including prolonged recovery and physiological stress. Novel strategies, such as Wide-Awake Local Anaesthesia No Tourniquet (WALANT), ultrasound-guided distal nerve blocks, and adjunctive approaches (vapocoolant spray, patient-controlled regional analgesia, cryoanalgesia, jet injectors), have emerged to address these limitations. This narrative review consolidates current evidence regarding the efficacy, applicability, and economic implications of these evolving anaesthesia techniques. Methods: A literature search was conducted across MEDLINE, Embase, CENTRAL, and Scopus databases up to 1 June 2025. Inclusion criteria were English-language original studies on WALANT, vapocoolant sprays, ultrasound-guided distal nerve blocks, or emerging adjunctive anaesthesia methods applicable to hand and upper limb surgery. Exclusion criteria included non-English publications and those without original clinical data. Two independent reviewers screened and selected studies, ensuring relevance and methodological quality. Results: WALANT can provide high patient satisfaction, cost savings of 70–85%, and allow for real-time functional testing during surgery. Ultrasound-guided nerve blocks provided targeted analgesia, preserved elbow function, reduced the need for sedation, and improved perioperative efficiency. Adjuncts such as vapocoolant sprays significantly decreased needle-injection discomfort, offering quick and economical analgesia for superficial procedures. Other emerging adjuncts, including patient-controlled regional anaesthesia (PCRA), cryoanalgesia, and jet injectors, offered additional patient-tailored pain management options, although with higher resource demands. Conclusions: The review highlights the transformative potential of WALANT and adjunctive techniques to enhance efficiency, patient experience, and cost-effectiveness in hand surgery. Despite clear benefits, optimal application requires tailored patient selection, clinician familiarity, and consideration of procedure-specific demands. Full article

Currently, flexible sensors based on electrochemical principles are predominantly limited to single-parameter detection, making it challenging to meet the demand for synchronous monitoring of multiple analytes in complex physiological environments. This study presents a 3D-printed flexible sensor for synchronous glucose/pH detection. Glucose was quantified via H₂O₂ oxidation current (GOD-catalyzed reaction), while pH was measured through polyaniline (PANI) resistance changes. The ionogel-based microneedle electrode ensures mechanical robustness. At 0.2 V, optimal signal decoupling was achieved: glucose oxidation current dominates, while PANI’s polarization effect is minimized. Neutral pH minimally affected glucose oxidase (GOD) activity, and low glucose concentrations induced negligible pH interference, ensuring orthogonality. In artificial interstitial fluid, the sensor showed glucose: linear response (0.5–2.5 g·L⁻¹, 0.288 μA·mM⁻¹·cm⁻²); pH: piecewise-linear sensitivity (0.155 Ω/pH·cm² for pH > 7; 0.135 Ω/pH·cm² for pH < 7). The design enables real-time multiparameter monitoring with high selectivity, addressing current limitations in flexible electrochemical sensors. Full article

Dromedary camels raised under semi-extensive management can act as One Health sentinels for environmental exposures and food chain surveillance, yet serum reference information remains scarce. Our objective was to provide the most comprehensive assessment to date of physiological and toxicological serum profiles in dromedary camels (Camelus dromedarius) from the Canary Islands. We included 114 clinically healthy animals of different sex, age, and reproductive status. Serum samples were analyzed for essential, toxic, and potentially toxic elements using inductively coupled plasma mass spectrometry (ICP-MS). In addition, a high-throughput multi-residue method based on QuEChERS extraction followed by UHPLC-MS/MS and GC-MS/MS was used to screen for 360 organic compounds, including pesticides, veterinary drugs, human pharmaceuticals, and persistent organic pollutants. Essential elements showed biologically consistent variations according to sex, age group, and pregnancy status. Males had higher levels of selenium and copper, while calves showed elevated concentrations of manganese and zinc. Pregnant females exhibited lower iron, zinc, and selenium levels, consistent with increased fetal demand. These results provide preliminary reference values for healthy camels, stratified by physiological status. In contrast, classical toxic elements such as arsenic, mercury, lead, and cadmium were found at very low or undetectable concentrations. Several potentially toxic elements, including barium, strontium, and rare earth elements, were detected sporadically but without toxicological concern. Only 13 organic compounds (3.6%) were detected in any sample, and concentrations were consistently low. The most prevalent was the PAH acenaphthene (55.3%), followed by the fungicide procymidone and the PAH fluorene. Notably, no residues of the usually detected 4,4′-DDE or PCB congeners were found in any sample. These findings confirm the low environmental and dietary exposure of camels under low-intensity farming systems and highlight their value as sentinel species for food safety and environmental monitoring. Full article

Adolescent athletes face unique nutritional challenges due to the simultaneous demands of growth, development, and athletic performance. This review synthesizes current evidence on energy and macronutrient requirements, hydration strategies, and key micronutrients, including iron, calcium, and vitamin D, which are essential for supporting health and performance in youth sport. It explores the physiological risks associated with low energy availability (LEA), while emphasizing the importance of carbohydrate and protein timing, quality, and distribution. The review also evaluates the role of dietary supplements and ergogenic aids, including creatine and energy drinks, highlighting safety concerns and advocating for a food-first approach. Practical strategies for nutrition education, interdisciplinary collaboration, and individualized care are presented to guide healthcare professionals, coaches, and caregivers in fostering sustainable, performance-supportive eating habits. By aligning intake with training demands and developmental needs, adolescent athletes can optimize performance, recovery, and long-term well-being. Full article

Controlled environment agriculture (CEA) has emerged as a vital solution to address the escalating global food demand amidst urbanization and diminishing arable land. However, the high energy consumption of CEA poses significant challenges for sustainable development. This paper provides a comprehensive review of the energy management models within CEA. The basic models of environmental factors such as light, temperature, humidity, and CO₂ concentration are introduced, highlighting their impact on plant growth and energy use. This paper elaborates on the coupling relationships between plant physiological activities and environmental control, facility environment and energy systems, and energy consumption and carbon emissions. Applications of energy management in CEA, including optimal energy scheduling, interaction with microgrids, and planning issues, are reviewed. Future research directions, such as multi-time-scale dynamic modeling, uncertainty modeling, and demand response (DR) modeling under market-oriented mechanisms, are also discussed. Full article

Artificial intelligence (AI) is revolutionarily shaping the entire landscape of medicine and particularly the privileged field of radiology, since it produces a significant amount of data, namely, images. Currently, AI implementation in radiology is continuously increasing, from automating image analysis to enhancing workflow management, and specifically, pediatric neuroradiology is emerging as an expanding frontier. Pediatric neuroradiology presents unique opportunities and challenges since neonates’ and small children’s brains are continuously developing, with age-specific changes in terms of anatomy, physiology, and disease presentation. By enhancing diagnostic accuracy, reducing reporting times, and enabling earlier intervention, AI has the potential to significantly impact clinical practice and patients’ quality of life and outcomes. For instance, AI reduces MRI and CT scanner time by employing advanced deep learning (DL) algorithms to accelerate image acquisition through compressed sensing and undersampling, and to enhance image reconstruction by denoising and super-resolving low-quality datasets, thereby producing diagnostic-quality images with significantly fewer data points and in a shorter timeframe. Furthermore, as healthcare systems become increasingly burdened by rising demands and limited radiology workforce capacity, AI offers a practical solution to support clinical decision-making, particularly in institutions where pediatric neuroradiology is limited. For example, the MELD (Multicenter Epilepsy Lesion Detection) algorithm is specifically designed to help radiologists find focal cortical dysplasias (FCDs), which are a common cause of drug-resistant epilepsy. It works by analyzing a patient’s MRI scan and comparing a wide range of features—such as cortical thickness and folding patterns—to a large database of scans from both healthy individuals and epilepsy patients. By identifying subtle deviations from normal brain anatomy, the MELD graph algorithm can highlight potential lesions that are often missed by the human eye, which is a critical step in identifying patients who could benefit from life-changing epilepsy surgery. On the other hand, the integration of AI into pediatric neuroradiology faces technical and ethical challenges, such as data scarcity and ethical and legal restrictions on pediatric data sharing, that complicate the development of robust and generalizable AI models. Moreover, many radiologists remain sceptical of AI’s interpretability and reliability, and there are also important medico-legal questions around responsibility and liability when AI systems are involved in clinical decision-making. Future promising perspectives to overcome these concerns are represented by federated learning and collaborative research and AI development, which require technological innovation and multidisciplinary collaboration between neuroradiologists, data scientists, ethicists, and pediatricians. The paper aims to address: (1) current applications of AI in pediatric neuroradiology; (2) current challenges and ethical considerations related to AI implementation in pediatric neuroradiology; and (3) future opportunities in the clinical and educational pediatric neuroradiology field. AI in pediatric neuroradiology is not meant to replace neuroradiologists, but to amplify human intellect and extend our capacity to diagnose, prognosticate, and treat with unprecedented precision and speed. Full article

During pregnancy, uterine circulation undergoes profound structural and functional adaptations to accommodate the dramatically increased metabolic demands of the growing fetus. Oxidative stress (OS) and inflammation have emerged as central regulators both physiologically, to drive vascular remodeling and angiogenesis, and pathologically, when dysregulated, to promote endothelial dysfunction, maladaptive extracellular matrix (ECM) remodeling, and heightened arterial stiffness. This review synthesizes insights into the molecular sources of reactive oxygen species (ROS) in the uterine vasculature, endothelial and immune-mediated inflammatory pathways, the bidirectional crosstalk between OS and inflammation, and their combined impact on vascular stiffness. We further discuss clinical implications for conditions such as preeclampsia and intrauterine growth restriction (IUGR), highlight circulating and imaging biomarkers of redox–inflammatory imbalance, and evaluate antioxidant and anti-inflammatory therapeutic strategies. Finally, we identify critical knowledge gaps and propose future research directions aimed at translating mechanistic understanding into personalized maternal–fetal care. For this narrative review, we searched the PubMed and Web of Science databases to identify all human and animal studies investigating OS and inflammation on uterine vasculature remodeling during pregnancy. Full article

This study investigates the influence of rear setback geometry and façade design parameters on microclimatic conditions, indoor thermal comfort, and energy performance in multi-story residential buildings in hot arid climates, addressing the growing need for climate-responsive design in regions with extreme temperatures and high solar radiation. Despite increasing interest in sustainable strategies, the combined effects of urban geometry and building envelope design remain underexplored in these environments. A coupled simulation framework was developed, integrating ENVI-met for outdoor microclimate modeling with Design Builder and EnergyPlus for dynamic building performance analysis. A total of 270 simulation scenarios were examined, combining three rear setback aspect ratios (1.5, 1.87, and 2.25), three window-to-wall ratios (10%, 20%, and 30%), three glazing types (single-, double-, and triple-pane), and two wall insulation states, using customized weather files derived from microclimate simulations. Global sensitivity analysis using rank regression and multivariate adaptive regression splines identified the glazing type as the most influential parameter (sensitivity index ≈ 0.99), especially for upper floors. At the same time, higher aspect ratios reduced peak Physiological Equivalent Temperature (PET) by up to 5 °C and decreased upper-floor cooling loads by 37%, albeit with a 9.3% increase in ground-floor cooling demand. Larger window-to-wall ratios lowered lighting energy consumption by up to 35% but had minimal impact on cooling loads, whereas wall insulation reduced annual cooling demand by up to 29,441 kWh. The results emphasize that integrating urban morphology with optimized façade components, particularly high-performance glazing and suitable aspect ratios, can significantly improve thermal comfort and reduce cooling energy consumption in hot arid residential contexts. Full article

Padel is a doubles racket sport played on an enclosed court, characterised by intermittent high-intensity efforts, frequent directional changes, and short recovery periods. This study aimed to analyse the evolution of physiological responses and neuromuscular fatigue in amateur padel players according to playing position (Right Side [RS] vs. Left Side [LS]) and match outcome (Win or Lose). A total of 52 padel players (35.6 ± 11.6 years) participated, competing in 13 matches. The mean match duration was 57.2 ± 15.7 min, with an average of 152.0 ± 40.4 points per match. Physiological variables were recorded during each set, and neuromuscular variables (countermovement jump [CMJ] and handgrip strength) were assessed before the match and after each set. No significant differences in physiological load were found between winners and losers or between RS and LS positions. However, differences in handgrip strength were observed at T1 (p < 0.05, d = −0.72) and T2 (p < 0.05, d = −0.59) (post-set testing), with LS players showing higher grip strength. Regarding the progression of physiological responses across the different sets, a progressive increase in cardiovascular load was observed within each subgroup, with significant differences across sets (set 1, set 2, and set 3) in several variables, including HR_peak, HR_avg, zone 1, zone 2, zone 3, and TRIMP_Edwards. No performance decline was observed in CMJ or handgrip strength in any of the groups analysed. These findings suggest that physiological responses increase throughout a match, particularly in the final sets, but no signs of neuromuscular fatigue (CMJ and handgrip) were observed, regardless of match outcome or playing position. These results highlight the need to include high-intensity scenarios and role-specific strategies in training to address the progressive physiological demands and positional differences in match play. Full article