Search Results (1,345)

This study evaluated the effect of a combined treatment of biostimulants on the recovery of low-maintenance turfgrass grown in central Chile. During the summer of 2025, a trial was conducted in Melipilla (central Chile) in an area planted with turfgrass sods consisting of a mixture of warm- and cool-season grasses under weekly mowing conditions. Three doses of the biostimulant treatment were applied at 15-day intervals, maintaining a controlled irrigation regime based on daily turfgrass evapotranspiration. The turf quality during the study was evaluated using vegetation indices based on RGB digital image analysis (VARI and TGI). During the trial period, only the treated group showed a significant improvement relative to its initial condition in one of the two evaluated indices (VARI). Comparative analyses across evaluation dates revealed significant differences between the treatment and the procedural control, which represented the non-maintained condition and the deterioration targeted by the trial, whereas no significant differences were detected with the irrigated control. These preliminary findings suggest that biostimulant treatments could represent a feasible tool for improving the appearance and recovery of turfgrass recurrently affected during summer conditions in urban areas of central Chile. However, future studies should include repeated damage–recovery events and incorporate multispectral indices, such as NDVI, to strengthen the robustness of the results. Full article

Seaweed-derived biostimulants are a promising strategy for improving crop performance in sustainable agriculture. In this context, this study evaluated the effects of foliar application of Kappaphycus alvarezii extracts, obtained from two Brazilian regions (São Paulo: Kal-SP and Santa Catarina: Kal-SC), at different concentrations (1%, 3%, 5%, and 7%) on the growth, biochemical profile, essential oil yield, and rhizosphere microbiome of Ocimum basilicum under field conditions. Morphological analysis indicated that the 5% and 7% concentrations increased plant height, biomass, root development, and inflorescence production, with biomass gains of up to 51% and essential oil production increases of up to 142% compared to the control. Biochemical responses varied by extract origin, with Kal-SC promoting greater increases in photosynthetic pigments, antioxidant activity, and carbon-related metabolites, whereas Kal-SP induced only minor metabolic changes. The algal biostimulant modulated essential oil yield and composition, promoting treatment-dependent shifts in major terpenoid compounds. Microbiome analysis showed no significant changes in alpha diversity, but significant shifts in beta diversity and functional groups, such as Bacillaceae, indicating rhizosphere reorganization. Overall, the effectiveness of K. alvarezii-based biostimulants depends on concentration and biomass source, highlighting their potential as sustainable agricultural bioproducts and the importance of standardized extraction for consistent outcomes. Full article

Climate change is emerging not only as a threat to global food production but also as a major driver of declining nutritional quality in food crops. Throughout this review, terms such as nutrient decline, imbalance, and nutritional quality changes are used to describe relative changes in the nutritional attributes of edible crop tissues, as reported in the source studies. Elevated atmospheric CO₂, altered rainfall patterns, shifts in solar radiation, and rising temperatures influence soil processes, plant metabolism, and genotype × environment interactions that determine nutrient composition and density. Evidence from controlled experiments, free-air CO₂ enrichment (FACE) studies, field trials, and meta-analyses suggests a recurrent tendency toward reduced concentrations of essential macronutrients and micronutrients, including protein, iron, zinc, and selected B-vitamins in a range of cereals, legumes, and horticultural crops, while responses remain context-dependent and are not universally observed across all nutrients, cultivars, or production systems. These reductions raise serious concerns for populations already experiencing widespread micronutrient deficiencies. This review synthesizes the current knowledge on the extent and mechanisms of climate-driven nutrient decline across major crops, highlighting variability among species, cultivars, and production environments. We also evaluate the potential health consequences, particularly heightened risks of anemia, impaired immunity, developmental challenges, and other deficiency-related disorders. Regions such as South Asia, Southeast Asia, and Sub-Saharan Africa are identified as highly vulnerable due to their strong dependence on nutrient-poor staples and existing burdens of hidden hunger. Furthermore, we assess key mitigation and adaptation pathways, including agronomic innovations, climate-smart agricultural practices, biofortification, advanced breeding strategies, and the emerging use of microbial and cyanobacterial biostimulants to enhance nutritional resilience in cropping systems. Finally, this review provides an integrated synthesis of climate-induced nutrient decline, its health implications for vulnerable populations, and priority actions needed to protect global food and nutrition security in the face of accelerating climate change. Full article

Wild maize (teosinte) has been reported to be less susceptible to biotic and abiotic stresses than its modern relative, corn. The composition of the teosinte root microbiome may be linked to traits such as drought tolerance and pest resistance. Trichoderma spp. are ubiquitous saprotrophic fungi found in the plant rhizosphere, enhancing host plant growth and crop productivity while alleviating biotic and abiotic stresses. The present study identified ten Trichoderma fungal isolates associated with the rhizosphere microbiome of teosinte (Zea mays spp. mexicana) and performed in vitro screening to assess both their multi-trait plant growth-promoting activities and their biological control potential against the phytopathogens Aspergillus flavus and Fusarium verticillioides. Additionally, interaction tests were conducted to evaluate the phytostimulant effect of Trichoderma spp. on maize (Zea mays) seed germination. Taxonomic and phylogenetic analysis identified five different Trichoderma species: T. rifaii (TA and TH); T. azevedoi (TB and TI); T. afroharzianum (TE); T. hamatum (TF and TG); and Trichoderma sp. (aff. bannaense) (TC, TD, and TJ). Partial least squares discriminant analysis revealed the isolates TF, TG, and TJ to have the highest potential for use as biocontrol and biostimulant agents. The present study is the first to examine Trichoderma species associated with the teosinte microbiome, and the results suggest that Trichoderma isolates are a potential sustainable alternative for improving maize cultivation. Full article

The increased demand for higher-quality, longer-lasting blueberries has led to the development of preharvest strategies to improve their structural integrity sustainably. This study analysed the effects of the foliar application of two biostimulant–calcium (Ca) combinations, using Ecklonia maxima extract (EM + Ca) and glycine betaine (GB + Ca), on yield, biometric, mechanical, and histological properties, as well as cuticular wax composition of blueberries. Both biostimulants increased yield per plant and fruit weight and size in ‘Duke’, with superior results for GB + Ca. Fruit yield increased by 80% with GB + Ca and 40% with EM + Ca. Histological analysis showed increases in cuticle thickness, epidermal cell area and thickness, and hypodermal cell area and area/perimeter ratio. This thicker, denser tissue ultimately improved blueberries’ mechanical properties. Specifically, ‘Draper’ berries treated with GB + Ca had 36%, 15%, and 20% higher values for flesh firmness, stiffness, and deformation work, respectively, relative to the control. However, cuticular wax accumulation was more pronounced with EM + Ca for the ‘Duke’ cultivar, increasing by 12%. Overall, GB + Ca had the greatest impact on blueberry structural quality and may represent a promising strategy to improve postharvest quality and commercial production. Full article

The transition towards sustainable agri-food systems necessitates the development of effective and technologically advanced biofertilizers and biostimulants capable of reducing reliance on synthetic agrochemicals while enhancing crop productivity. Photosynthetic microorganisms, including cyanobacteria and microalgae, represent promising biological platforms owing to their extensive metabolic potential, their ability to synthesize high-value bioactive compounds, and, in certain cases, their capacity for atmospheric nitrogen fixation. These properties make them particularly valuable for enhancing plant growth and improving tolerance to abiotic and biotic stresses. In this study, a systematic review was conducted to identify diverse cyanobacterial and microalgal taxa with demonstrated roles in plant growth promotion and stress mitigation through multiple mechanisms and adaptive traits. A subset of these microorganisms was subsequently curated into a targeted database and subjected to bioinformatics analyses, leading to the identification of key metabolic pathways associated with stress response and plant growth promotion. Full article

Extracellular DNA (eDNA) has gained attention as a danger signal between organisms because of the ecological implications of this mechanism and its great potential as a biological modulator in agriculture. Self-DNA and non-self DNA have been evaluated earlier, both as plant immune system elicitors. Here we show the effect of eDNA extracted from the bacterial phytopathogen Clavibacter michiganensis applied to tomato plants in different concentrations (50, 100 and 150 µg mL⁻¹). Monitoring morphology of the plants, spectrophotometric determinations and RT-qPCR assays showed a dose-dependent effect on plant growth and root development, activation of antioxidant enzymes such as catalase and superoxide dismutase, biosynthesis of secondary metabolites, including phenolic compounds and flavonoids, and differential expression of genes related to plant stress response, such as chalcone synthase and phenylalanine ammonia-lyase. Lower concentration treatments showed an increment in the variables as beneficial responses for agricultural practices, and the higher concentration (150 µg mL⁻¹) showed reduced or no effects on the evaluated variables. This work represents a step forward in the development of effective and more sustainable agricultural technology in crop production. Full article

Plant-parasitic nematodes (PPNs) rank among the most economically destructive soilborne pathogens worldwide, causing annual crop losses estimated at USD 125–175 billion. Traditional management of plant parasitic nematodes has depended significantly on synthetic nematicides; however, increasing regulatory constraints, environmental pollution, and the rise of resistant nematode populations have generated an urgent need for sustainable alternatives. Humic substances (HS), comprising humic acids, fulvic acids, and humins derived primarily from leonardite and lignite, represent biologically active components of soil organic matter. Their different functional groups, like carboxylic, phenolic, and carbonyl groups, have direct nematicidal and nematostatic effects by stopping eggs from hatching, slowing down juvenile development, and lowering infectivity. They also indirectly improve soil structure, nutrient bioavailability, and the composition of the rhizosphere microbiome. Plant growth-promoting rhizobacteria (PGPR), particularly Bacillus spp. and Pseudomonas spp., suppress PPN populations through antibiotic biosynthesis, cuticle-degrading hydrolytic enzymes, nematostatic volatile organic compounds, and elicitation of induced systemic resistance (ISR). This review methodically analyzes the individual and synergistic processes by which HS and PGPR inhibit PPNs and enhance plant growth. Humic compounds strongly promote PGPR rhizosphere colonization, augmenting microbial metabolic activity and bioinoculant stability, hence producing combinatorial suppressive effects unattainable by either input independently. The combined HS-PGPR approach is reliable and environmentally sustainable for comprehensive nematode control, requiring multidisciplinary research to achieve global sustainable agriculture. Full article

Amino acids are organic compounds that serve as the fundamental building blocks of proteins and are additionally responsible for a multitude of other biological functions. This review synthesizes recent evidence elucidating that amino acids function as vital players in nitrogen transport, stress defense, and perhaps most intriguingly as signaling molecules. For example, glutamate triggers calcium signals through GLR receptors to guide root growth and pollen tubes. Others, like proline and glutathione, protect cells from drought, salt, and oxidative damage. Aromatic and sulfur-containing amino acids also feed into the production of hormones (auxin, ethylene) and a wide range of defense compounds. Beyond metabolism, we highlighted how plants sense amino acid status via ancient sensors such as PII and the TOR pathway, which fine-tune growth and resource allocation. Understanding this hidden side of amino acids opens new doors for agriculture. We discussed how these insights could lead to smarter biostimulants, gene-edited crops with better nutrient efficiency, and nano-based delivery systems. In short, amino acids are not just food for plants—they are signals, shields, and switches that shape how plants grow and cope with stress. Full article

Application of microbial biostimulants can reduce the need for applying mineral fertilisers, particularly those with limited availability or high environmental impact, like phosphate fertilisers. A consortium of bacterial strains able of solubilizing phosphorous (P) was applied together with reduced P fertilization (60% of standard dose) carried out with either a complex fertilizer (CF) or simple P fertilizer (SF) and compared to standard fertilization rates. Trials with field grown cucumber plants were carried out for four years. The application of the microbial inoculum with reduced dose of both fertilizers did not negatively impact on plant physiological parameters and on yield. The evaluation of the inoculation impact on soil microbial activity, carried out on plants grown in rhizoboxes, showed that the aforementioned inoculation, combined with reduced doses of both fertilizers, significantly increased microbial activity in the rhizosphere compared to fertilizers applied at full doses without inoculation. The results suggest that the integration of microbial biostimulants to a reduced mineral fertilization is a strategy that can be applied also in the long term, leading to a significant reduction in the use of mineral fertilizers and a positive economic and environmental impact. Full article

Low-molecular-weight chitosan derivatives emerged as promising plant growth biostimulants due to their favorable properties, such as biocompatibility, antibacterial and antifungal activity, enhancement of stress resistance, and yield improvement. In the present study, we evaluated the effect of pea seed priming with two types of chitooligosaccarides (aminochitooligosaccaride and chitooligosaccaride hydrochloride) applied at concentrations of 100 and 500 mg/L under non-stress conditions and 50 mM chronic NaCl stress. We characterized the seed surface topology by atomic force microscopy, the germination process by evaluation of seed germinability and synchrony, root emergence, seed imbibition capacity and ion leakage. Early plant growth and physiological performance were further evaluated in 14-day-old seedlings by measuring leaf water potential, Na⁺ accumulation in roots and leaves, photosystem II activity, leaf pigment content, and membrane stability. The results revealed changes in seed coat topology, i.e., higher surface roughness in 100 and 500 mg/L chitooligosaccaride hydrochloride and 500 mg/L aminochitooligosaccaride primed variants. Concentration-dependent effects of the two chitooligosaccarides under both non-stress and salt stress conditions were evident in 14-day-old seedlings. Under chronic salt stress, seed priming with 100 mg/L chitooligosaccharide hydrochloride and 500 mg/L aminochitooligosaccharide produced the most pronounced improvements in the primary photochemical reactions of photosynthesis, particularly the performance index on an absorption basis and the total performance index. Moreover, the investigated chitooligosaccharide, particularly chitooligosaccaride hydrochloride, preserved membrane integrity and maintained flavonol and anthocyanin levels, indicating a strong protective effect against salt stress. Overall, the data indicate beneficial effects on pea physiological status following seed priming with chitooligosaccarides under chronic salt stress conditions. This highlights the approach as a promising strategy for enhancing plant resilience in challenging environments, and it is worth further investigation and verification at the whole-plant level. Full article

Soybean is one of the most important crops worldwide, but its productivity is frequently challenged by abiotic stresses such as drought and heat, which impair physiological and metabolic processes. Biostimulants have emerged as sustainable tools to improve plant performance under adverse conditions. This study evaluated the effects of foliar application of a new biostimulant, “SB”, on soybean photosynthetic efficiency, antioxidant metabolism, biometric traits, and grain yield. SB was applied at different doses (0.5, 1.0, 1.5, and 2.0 L ha⁻¹) at the V₄ and R₁ growth stages during two seasons (2023/2024 and 2024/2025). Foliar SB application enhanced soybean leaf chlorophyll levels, RuBisCO activity, and gas exchange parameters, resulting in higher photosynthetic rates, carboxylation efficiency, and water use efficiency. In addition, foliar SB application reduced hydrogen peroxide and malondialdehyde accumulation, indicating lower oxidative damage and improved redox balance. These physiological and metabolic improvements contributed to greater root development and plant height and significant increases in yield components. Grain yield was consistently improved by all SB application rates, but the 1.5 L ha⁻¹ dose produced the most stable and positive effects across both seasons, with an average increase of more than 500 kg ha⁻¹ compared to the control. Overall, foliar SB application proved to be an efficient and promising management strategy to enhance soybean resilience and productivity under variable climatic conditions. Full article

Microalgae-based biostimulants are gaining increasing interest worldwide for promoting sustainable agriculture. The environmental risks associated with synthetic agrochemicals can be mitigated by using microalgae to enhance crop yield and quality. Cumin (Cuminum cyminum L.) is an herbaceous plant and ranks among the most popular seed spices worldwide. It is characterized by a low germination rate and poor seedling establishment, which negatively impact overall crop yield. To address these challenges, the present study investigates the potential of Chlorococcum sp. aqueous extract as a sustainable and cost-effective solution to overcome cumin seed dormancy and enhance germination. Results showed that Chlorococcum sp. exhibits a notably rapid growth rate (0.45 day⁻¹) and high biomass productivity (1.51 g/L/day). Additionally, the biochemical composition of the extract revealed a high concentration of bioactive compounds, including polyphenols (63.46%), flavonoids (29.36%), and Indole-3-acetic acid (5.38%), which make it an eco-friendly biostimulant for agricultural applications. Regarding germination, a single seed treatment with doses of 0.5 g/L and 1 g/L was efficient in achieving final germination percentages of 100% and 96.66%, respectively, and significantly increased the seedling vigor index and photosynthetic pigment content. Furthermore, these concentrations stimulated the synthesis and accumulation of key primary metabolites, including proteins and polysaccharides, while increasing phenolic and flavonoid levels compared to the control, suggesting enhanced growth and improved antioxidant defenses against environmental stressors. Overall, these findings highlight that Chlorococcum sp. aqueous extract serves as an innovative biological approach to overcoming cumin seed dormancy and enhancing germination, offering an alternative and sustainable solution to conventional synthetic fertilizers. Full article

Soil salinization, intensified by climate change, reduces soil quality and crop yields, posing a severe threat to food security. The present study focuses on the effects of two doses of a biostimulant, based on plant protein hydrolysates, on improving the root system and the quality of NaCl-affected soil. For this purpose, several experiments were conducted on Solanum lycopersicum plants that were grown for 60 days under four salinity conditions, obtained by combining two salinity levels and two irrigation water types (a total of 36 treatments). Several physical and chemical soil properties and root characteristics were evaluated, and it was shown that the application of the biostimulant (BALOX^®) significantly increased root length and total root area, even under high salinity conditions. An increase of up to 70% over the control was achieved, mostly in roots smaller than 2 mm in diameter, which are primarily responsible for nutrient absorption. It was also revealed that BALOX^®’s interaction with the root system favorably influenced soil properties, particularly Cation Exchange Capacity (CEC). Likewise, the Aggregate stability (AS) increased up to 36%, and the percentage of organic matter (OM) up to 6.4%. The CEC increased by 66–72% with the biostimulant application, and there were reductions in soil salinity and Na⁺ and Cl⁻ concentrations (20%, 19%, and 24%, respectively). In addition, BALOX increased the area and length of fine roots, thereby expanding the rhizosphere and enhancing its interaction with the soil. The use of the biostimulant may help prevent soil degradation and contribute to tomato plants’ tolerance mechanisms under salt stress. Full article

Climate variability and widespread synthetic agrochemical use have increased interest in biostimulants (BS) that enhance plant growth, stress tolerance, and yield by stimulating natural plant processes. A two-site field study, conducted under no-till and tilled systems, evaluated the effects of the foliar biostimulant “Source” on soybean growth and yield at three phosphorus (P) rates (0%, 50%, and 100% of soil test recommendations) because of its potential to replace phosphorus inputs. A complementary greenhouse study was conducted to evaluate the effect of the biostimulant on different soybean hybrids. Measured at various dates after planting (DAP), leaf relative water content (LRWC) and normalized difference vegetation index (NDVI) mostly showed insignificant responses to P treatments, but significant responses to BS. Grain yield increased significantly with individual BS and P applications in both tillage systems. Under no-till conditions, BS increased yield by 13.0% (3.05 vs. 2.70 Mg ha⁻¹), and P100 increased yield by 13.5% (3.0 vs. 2.65 Mg ha⁻¹). Under tilled conditions, BS and P100 increased yield by 19.6% (2.75 vs. 2.30 Mg ha⁻¹) and 19.2% (2.72 vs. 2.28 Mg ha⁻¹), respectively, compared with the control. Yield gains were primarily driven by increased pod density and grain number. Greenhouse experiments supported these trends, with BS-treated plants producing more grains per plant (187.6 vs. 171.3) and higher yield per plant (28.8 vs. 25.7 g). Results indicated that biostimulant application improved physiological performance and increased soybean yields, comparable to full-rate phosphorus, highlighting its potential as a sustainable approach under increasing environmental and input-related challenges. Full article