Search Results (3,292)

Wheat stem rust, caused by Puccinia graminis f. sp. tritici (Pgt), threatens food security owing to the pathogen’s high virulence and evolutionary variability, as well as the risk of selecting fungicide-resistant forms. For the first time in Russia, we performed a comparative analysis of virulence and genetic diversity in fungicide-target genes (SdhA–SdhD, Cyp51) in 35 monopustular Pgt isolates collected from regions with contrasting fungicide pressure (Saratov, n = 12; Chelyabinsk, n = 12; Moscow, n = 11). Phenotyping on an extended set of nearly isogenic lines carrying different Sr genes revealed regional specificity in effective resistance genes (Sr13, Sr26, Sr31, Sr32, Sr35), underscoring the vulnerability of genetic protection in Russian commercial cultivars. Targeted amplicon sequencing using the Oxford Nanopore Technologies platform produced 175 amplicons (~38,290 long reads, N50 = 1.06 kb), identifying 87% synonymous SNPs, 12% non-synonymous substitutions, and a single nonsense mutation in SdhA (position 580). The Cyp51 and SdhA genes were the most polymorphic. Recurrent non-synonymous mutations in these genes were detected in Pgt clones from regions with intensive agriculture and high use of chemical plant protection products. These results support the need for predictive breeding of resistant cultivars and adaptation of chemical control within integrated Pgt management in the European part of Russia. Full article

The increasing demand for sustainable agricultural practices has intensified the search for natural alternatives for the control of plant pathogens. This study aimed to evaluate the in vitro inhibitory activity of plant extracts obtained from agro-industrial byproducts, including pomegranate peel (Punica granatum), chestnut peel (Castanea sativa), and grape pomace (Vitis vinifera), against the bacterium Pseudomonas savastanoi and the fungus Colletotrichum fioriniae. Antibacterial activity was assessed using the disk diffusion and Minimum Inhibitory Concentration (MIC) methods. Antifungal activity was evaluated for all extracts through mycelial growth inhibition, spore quantification, and viability assays. Based on its overall antimicrobial performance and extraction yield, the P. granatum extract was selected for evaluation in a detached olive fruit bioassay. The results showed that the pomegranate peel extract exhibited an inhibition halo of $9.80\pm 0.10$ mm against Pseudomonas savastanoi, with a MIC of 5.0 mg/mL and a predominantly bacteriostatic effect. Regarding antifungal activity, the Castanea sativa extract showed the highest inhibition of mycelial growth, reaching 27.27% after 3 days and reducing sporulation by up to 44.36%, whereas the Punica granatum and Vitis vinifera extracts exhibited only moderate inhibitory effects. In the sporulation assay, all extracts reduced spore production and increased early conidial germination, a transient response not accompanied by enhanced fungal development. In the detached olive fruit bioassay, pre-treatment with the P. granatum extract delayed disease progression and reduced final disease severity from 100% in the positive control to 64%, whereas simultaneous application of the extract and the pathogen did not provide a protective effect. Overall, the findings highlight the potential of plant-derived agro-industrial byproducts as sources of bioactive compounds for sustainable plant disease management strategies. Full article

Flax lignans are abundant plant secondary metabolites in flaxseed, with secoisolariciresinol diglucoside (SDG) content reaching 0.61–1.33% of seed dry weight—substantially higher than levels reported for sesame seeds (0.01–0.03%), cereals (<0.01%), and most legumes (<0.005%). These levels make them natural bioactive compounds with considerable application potential. This review summarizes the biosynthetic pathways, key enzymes, and regulatory mechanisms of flax lignans; describes their major biological activities, including antioxidant, anti-inflammatory, anticancer, cardiovascular protective, and metabolic regulatory effects and possible mechanisms of action; and analyzes current progress in extraction, purification, analytical detection, and application. Key limitations in industrial application include complex extraction procedures, high production costs, lack of unified quality standards, and insufficient clinical evidence. Advanced strategies such as synthetic biology approaches for heterologous production, molecular breeding for high-lignan varieties, and green extraction technologies are discussed as promising solutions. This review is intended to serve as a conceptual reference for subsequent mechanistic studies, product development, and industrial translation of flax lignans in food, pharmaceutical, and cosmetic applications. Full article

Persistent global warming significantly impacts crop phenology and productivity, with perennial fruit trees facing heightened challenges due to their long life cycles and complex heat stress accumulation. Apple and pear, which hold substantial economic and nutritional value, are particularly vulnerable to high temperatures, manifesting as accelerated phenology, impaired floral organ development, disrupted pollination and fertilization, and insufficient fruit coloration—all of which severely compromise fruit quality and commercial value. Although recent advances have been made in elucidating heat stress signal transduction and regulatory networks in model plants such as Arabidopsis and rice, research on heat stress responses in apple and pear remains limited. This review systematically synthesizes the physiological responses, gene expression regulation, and protective cultivation strategies under high-temperature stress in apple and pear, aiming to provide a theoretical foundation for thermotolerance breeding and the establishment of heat stress regulatory networks, thereby supporting sustainable production in the context of global warming. Full article

Mosquitoes are significant public health insects responsible for numerous vector-borne diseases. Injudicious chemical use has developed metabolic and genetic resistance in mosquitoes. This study evaluated the repellent efficacy of five essential oils (EOs) from local plants [Mentha spicata I, Ruta tuberculata, Mentha spicata II, Lavandula latifolia, and L. stoechas]. M. spicata I and R. tuberculata EOs were extracted using Clavenger hydrodistillation. Simultaneously, the remaining EOs were obtained through a local extraction distillation unit. Chemical composition was identified using GC-MS. Repellency was assessed using the arm-in-cage method, by rearing Culex pipiens s.l. and Aedes albopictus in the laboratory. Additionally, mixtures containing 15% DEET, 15% IR3535, 5% vanillin, and 10% M. spicata I EO were tested. Both mosquito species were clearly repelled by all EOs. M. spicata I was most potent at 10% concentration. The complete protection time (CPT) at 10% M. spicata I EO was increased using blended mixtures containing 15% DEET, 15% IR35353, or 5% vanillin. Regional variations in M. spicata EOs were detected. This study may serve as a foundation for cosmetic manufacturers and pest control products. Developing botanical and hybrid (natural and synthetic) formulations could provide strong protection against insect bites, particularly from disease-vector mosquitoes, helping to prevent vector-borne diseases. Full article

Long-term winter wheat monoculture leads to the deterioration of plant physiological performance and reduced productivity; however, the effectiveness of measures aimed at mitigating these adverse effects has not yet been comprehensively evaluated. The objective of this study was to assess the effects of crop sequence, crop protection intensity, and variety selection on the plant physiological parameters, grain yield, technological grain quality, and mineral composition of winter wheat grain, as well as to determine the extent to which yield losses caused by long-term monoculture can be compensated. The study was conducted during three growing seasons (2022/2023–2024/2025) in a long-term field experiment established in 1967 in Bałcyny, Poland, using a three-factor design with three replications, including two crop sequence systems, three crop protection levels, and two winter wheat varieties. Winter wheat grown in monoculture showed significantly lower leaf greenness index values and grain yield (by 41.4%; p < 0.05) compared with crop rotation. In contrast, monoculture significantly increased grain protein content (by 5.8%), gluten content (by 6.6%), Zeleny sedimentation index (by 20.8%), and the concentrations of nitrogen and zinc in grain (p < 0.05). Crop protection significantly enhanced grain yield; however, it did not completely eliminate the negative effects of long-term monoculture. The variety Revolver produced significantly higher grain yield and leaf greenness index values than Emil, whereas Emil produced grain with significantly higher protein, gluten, and nitrogen contents. The Monoculture Compensation Efficiency (MCE) index showed that crop protection practices enabled the recovery of 63.8–76.2% of yield losses caused by long-term monoculture. The results indicate that integrated crop protection can only partially mitigate the adverse effects of continuous monoculture and cannot replace the benefits associated with the implementation of a proper crop rotation system. Full article

The Internet of Plants (IoP) represents the convergence of Artificial Intelligence (AI), Big Data analytics, and the Internet of Things (IoT) within protected agricultural systems. This study presents an IoP platform designed to collect, process, and analyze real-time environmental data using specialized IoT sensors capable of monitoring critical variables, including carbon dioxide concentration (CO₂), pH, air temperature, and relative humidity in hydroponic production systems. The proposed framework integrates advanced machine-learning algorithms, including Random Forest Regressor and Long Short-Term Memory (LSTM) neural networks, to process large volumes of environmental data and support crop management. In addition, the platform incorporates Vapor Pressure Deficit (VPD) and Growing Degree Days (GDD) analyses to provide crop-specific recommendations and support informed decision-making. This platform establishes a benchmark for smart agriculture in Mexico’s Laja–Bajío region, facilitating informed decision-making and maximizing the sustainability of food systems. Experimental validation was conducted under both controlled and semi-controlled environments using Swiss chard (Beta vulgaris subsp. cicla L.) and lettuce (Lactuca sativa L.) cultivated in hydroponic systems. These environments represented contrasting climatic conditions, allowing evaluation of platform stability and forecasting performance under varying thermal regimes. The Random Forest Regressor model, trained using growth chamber data consisting of 19,836 valid observations, reproduced the deterministic VPD relationship with a coefficient of determination (R²) of 0.90 and a root mean square error (RMSE) of 0.08 kPa, confirming internal consistency and identifying temperature as the dominant contributing variable rather than predicting an independent outcome. The dynamic alarm system, integrated with crop phenological stages, demonstrated greater effectiveness than conventional static-threshold approaches by generating alerts according to crop developmental requirements. Furthermore, the web-based visualization platform enabled users to interpret environmental conditions through intuitive graphical representations, facilitating decision-making without requiring specialized technical expertise. The results demonstrate the feasibility of the IoP platform as a comprehensive environmental management tool for protected agricultural systems. The proposed framework provides a scalable solution for precision agriculture applications in the Laja–Bajío region of Mexico and in other regions with similar production systems. Full article

Vineyards and wineries generate seasonal, wet and compositionally variable side-streams whose safe use is constrained by rapid spoilage, contaminants, fragmented regulation and scale. This EU-focused structured narrative review synthesised 90 scientific and official sources and proposes an integrated decision framework rather than another catalogue of valorisation routes. The framework applies a non-compensatory sequence: characterise and stabilise the batch, test route-specific hazards, assign evidence-level and technology readiness, verify legal eligibility, define a safe fallback, and only then compare material flows, environmental burdens and risk-adjusted economics. Current implementation evidence is strongest for controlled composting, conventional wastewater treatment, anaerobic digestion and selected grape-seed oil, polyphenol and heat-integrated biochar operations; clinical, plant-protection and several novel-extract claims remain product- and context-specific. Illustrative ENPV cases show that high-value extraction can offer greater upside but lower robustness than compost/biochar when moisture, transport, rejection and price uncertainty are included. The framework provides an auditable basis for pilot design, regional cooperation and data collection, while explicitly requiring industrial and multi-season validation before investment or product approval. Full article

Rumen biohydrogenation is a microbial process that converts dietary unsaturated fatty acids into more saturated products and produces a range of intermediates that can affect animal products and rumen fermentation. This review summarizes current knowledge on the main biohydrogenation pathways, the microbial groups involved, and the nutritional and breeding strategies used to regulate this process. Particular attention is given to the transformation of C18 fatty acids, the formation of vaccenic acid and conjugated linoleic acid, the trans-10 shift, and the links between lipid metabolism, hydrogen use, and methane formation. Evidence indicates that diet composition, lipid source, plant secondary metabolites, rumen-protected fat technologies, microbial interventions, and host-related factors can all influence biohydrogenation outcomes. These strategies can improve the fatty acid profile of meat and milk, but their effects are context-dependent and vary with animal species, diet, and rumen microbial structure. Important gaps remain, including the identification of active microbial populations, the functions of many transient intermediates, and the extent to which changes in biohydrogenation directly contribute to methane mitigation. Clarifying these mechanisms is essential for improving the nutritional quality of ruminant products and the sustainability of production systems. Full article

Abiotic stresses increasingly threaten crop productivity, whereas reliance on chemical and resource-intensive interventions can compromise environmental sustainability. Existing literature identifies Trichoderma spp. as multifunctional biocontrol agents, biofertilizers, and microbial biostimulants capable of influencing plant growth, stress signaling, and rhizosphere processes; however, evidence remains fragmented across strains, crops, formulations, and stress conditions. This review aimed to integrate current knowledge on Trichoderma-mediated resilience to salinity, drought, heavy metals, temperature extremes, and emerging pollutants, while distinguishing experimentally validated mechanisms from statistical associations and conceptual inference. It evaluates constraints governing reproducibility from controlled studies to field deployment. The synthesis shows that selected crop–strain systems improve root architecture, photosynthesis, antioxidant regulation, osmotic adjustment, nutrient acquisition, ion homeostasis, hormonal balance, and stress-responsive gene expression. Benefits arise through coordinated delivery, root colonization, metabolite and protein signaling, physiological reprogramming, and rhizosphere modulation. Nevertheless, microbiome co-occurrence patterns do not establish causal network repair, evidence for broad heat and cold protection remains limited, and biochar co-application should not be interpreted as a carrier formulation without direct validation. Future progress requires strain- and crop-specific screening, mechanistic gene and protein studies, standardized formulations, combined-stress experiments, multi-location field trials, biosafety evaluation, and farmer-level economic assessment to develop reliable precision microbial technologies. Full article

Essential oils are increasingly investigated for their potential use in plant protection because of their insecticidal, fumigant, repellent, behavioural, and antifungal activities. This narrative review with a structured literature search synthesizes evidence relevant to European plant protection, focusing primarily on stored-product pests, with additional consideration of pests and plant pathogens outside storage systems, stage-specific susceptibility, formulation, practical limitations, and the EU regulatory framework. EO efficacy was strongly influenced by chemical composition, concentration, formulation, exposure time, developmental stage, target species, and environmental conditions. The strongest evidence was identified for stored-product pests (Coleoptera), although stage-specific susceptibility varied among species. Under field conditions, the practical application of essential oils remains challenging due to their high volatility, short persistence, sensitivity to environmental conditions, potential phytotoxicity, formulation requirements, and limited field validation. Methodological differences among assay types also limit direct comparison across studies. In the EU, regulatory acceptance remains substance-specific, with whole essential oils assessed separately from their individual volatile constituents. Most available evidence is derived from laboratory studies, while greenhouse and field validation remains limited. No formal methodological quality or risk-of-bias assessment of individual studies was performed. Overall, essential oils should be regarded as complementary, application-specific tools whose contribution to more sustainable plant protection depends on improved standardization, formulation, field validation, safety, economic viability, and regulatory acceptance. Full article

Tropical forests sustain exceptional biodiversity and regulate global carbon and water cycles, yet degradation and incomplete recovery increasingly compromise these functions. We integrated bibliometric mapping with structured systematic synthesis to characterize research at the tropical-forest degradation–restoration interface, identify its most influential contributors and publications, and evaluate evidence on drivers, interventions, monitoring, and knowledge gaps. This study addresses three guiding scientific questions: (i) How has the field developed over time and across geographic space? (ii) Which authors, institutions, journals and publications have been most influential? (iii) What does the evidence indicate about degradation drivers, restoration strategies, monitoring approaches and knowledge gaps? Scopus and Web of Science were searched for peer-reviewed articles and reviews published from 1980 to 2025. After deduplication and PRISMA-based screening, 1075 publications were analyzed bibliometrically, and the 400 most-cited studies were coded against twenty predefined questions. Scientific output increased by 10.68% annually, with 483 publications appearing during 2020–2025. The corpus comprised 318 journals and 4470 authors. Forest Ecology and Management was the leading source (141 publications; 13.1%), while the twenty most productive journals accounted for 44.3% of the corpus. Brancalion P.H.S. was the most productive author (23 publications), followed by Chazdon R.L. and Tabarelli M. (20 each). Citation influence was concentrated: the twenty most-cited documents received 25.7% of all citations, led by Ribeiro M.C. (3339 citations), whereas Hua F. achieved the highest publication-year-normalized citation score among this group. Agricultural expansion, pasture establishment, and logging were the most frequently reported degradation pressures, but interactions among logging, fire, drought, and fragmentation were rarely quantified. Restoration evidence supported a context-dependent continuum from natural regeneration to assisted and active interventions, although planting and enrichment were more visible than direct passive–active comparisons. Carbon, biomass, plant diversity, and forest structure dominated outcome assessment, whereas fauna, ecological interactions, governance, and socioeconomic dimensions received less attention. Monitoring relied mainly on satellite imagery and field inventories, with limited evaluation of tool performance. Long-term trajectories and evidence from Africa, Southeast Asia, seasonally dry forests, and montane systems remained scarce. Overall, the field is rapidly consolidating but remains geographically and thematically uneven. Future research should quantify interacting degradation processes, evaluate multidimensional and long-term recovery, connect remote sensing with ecologically meaningful field indicators, and integrate governance and social conditions into restoration planning. The synthesis supports preventing further degradation and treating restoration as a context-specific complement, rather than a substitute, for protecting remaining native forests. Full article

Background: Phytosterols are natural compounds that provide health benefits by lowering LDL cholesterol levels, but they are susceptible to thermo-oxidative degradation during food storage and processing. Encapsulating them in liposomes could increase their stability and bioavailability. The aim of this study was to examine the stability of phytosterols encapsulated in liposomes during heating of mayonnaise at temperatures simulating storage and frying conditions. Methods: Mayonnaise containing 2, 4, 6, and 8% liposomes encapsulating stigmasterol oleate was prepared and heated at 60 °C for 8 h and at 180 °C for 30, 60, 90, and 120 min. The fat content, percentage composition of fatty acids and levels of oleic acid and stigmasterol were determined in each tested mayonnaise. Results: The fat content of mayonnaise with liposomes heated at 60 °C for 8 h was 97–98%. During heating at 180 °C for 60 min, it reached 100%, and after 120 min, it decreased to 75%. The oleic acid content of liposome-enriched mayonnaise remained stable during heating. The greatest loss of stigmasterol was observed in the control sample without liposomes, in which the sterol content decreased 79%. After heating mayonnaise with liposomes, the decrease in stigmasterol ranged from 3 to 25% at 60 °C for 8 h and from 90% to 100% at 180 °C for 90 and 120 min. Conclusions: Enriching mayonnaise with phytosterol esters encapsulated in liposomes can effectively improve the thermal-oxidative stability of plant sterols during short-term heating, but does not protect the product from the loss of these sterols during prolonged, intense heating. Full article

Greenhouse strawberry production has rapidly increased around the world, since greenhouse production allows for protection from adverse weather events and optimized climate control. During the winter months, supplemental electric lighting is essential to maintain production. However, the use of supplemental lighting can account for up to 30% of operating expenses, impacting the profitability of production. In addition, strawberries are prized for their taste, so maintaining fruit quality during production is essential. This study examined the impact four different supplemental overhead LED spectral lighting treatments (red–blue (95% red, red-rich), pink, warm white, and white) and two different irrigation electrical conductivity (EC) levels (low = 2 mS cm⁻¹ and high = 3 mS cm⁻¹) on morphology, physiology, fruit quality and electricity costs in two strawberry cultivars—cv. ‘Albion’ and cv. ‘Favori’—grown in rockwool during the 2023–2024 winter greenhouse season in Harrow, Ontario, Canada. The results showed that plants exposed to a board spectrum generally had higher photosynthetic rates and vegetative biomass. However, a threshold was observed when the green light fraction was above 24% (i.e., plants grown under the white-light treatment), resulting in reductions in photosynthesis and vegetative biomass. Although differences in photosynthetic capacity and biomass were observed, there was no impact of the light spectrum or irrigation EC on yield in either cultivar. Compared to the red–blue spectrum, plants grown under pink and warm white were observed to have lower titratable acidity (TA) and higher ratios of total soluble solids (TSSs) relative to TA. Importantly, it was determined that due to the higher efficacy of the red–blue supplemental lighting fixtures, the red-rich spectrum resulted in the lowest estimated lighting electricity cost per kilogram under the tested conditions. This study suggests that the use of supplemental high-efficacy, narrow wavelength, red-rich supplemental lighting can maintain yield and improve economic feasibility for greenhouse-grown strawberries. Full article

Tomato production is highly affected by salt stress conditions, especially under protected cultivation systems using low-quality irrigation water. This study assessed the effects of increasing electrical conductivity (EC) levels of the nutrient solution (2, 4, 6, and 8 dS m⁻¹) on growth, biomass distribution, RGB-based spectral indices, physiological traits, petiole sap composition, and leachate chemistry in tomato plants (Solanum lycopersicum cv. Tiny Tim) cultivated in containers within a bamboo nethouse under tropical conditions. The results obtained reported that increasing salinity significantly reduced fresh and dry biomass (61.72 and 61.55% respectively) across all plant organs, with fruits showing the highest sensitivity. Leaf area index (LAI), SPAD values, leaf nitrogen (N) concentration, leaf relative humidity (LRH), and water uptake also declined progressively under saline conditions. In contrast, root and leaf biomass allocation increased under the highest salinity level. Spectral analyses reported reductions in red and blue RGB components and normalized red and blue index values, while normalized green index increased under higher salinity levels. Petiole sap analysis showed a progressive accumulation of sodium (Na⁺) and chloride (Cl⁻) together with reductions in nitrate (NO₃⁻-N) and potassium (K⁺) concentrations, particularly during the reproductive stage. In contrast, °Brix, calcium (Ca²⁺), and phosphorus (P) concentrations increased under salinity, particularly during the reproductive stage. Leachate analyses confirmed salt accumulation in the substrate and reduced plant water uptake. Overall, petiole sap analysis and RGB-based spectral indices provide a rapid, non-destructive, and cost-effective tool for the early detection of salinity stress in tomato, enabling timely irrigation and fertigation adjustments to improve crop performance and resource-use efficiency under saline conditions. Full article