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15 pages, 2270 KiB  
Article
Soil Infiltration Characteristics and Driving Mechanisms of Three Typical Forest Types in Southern Subtropical China
by Yanrui Guo, Chongshan Wan, Shi Qi, Shuangshuang Ma, Lin Zhang, Gong Cheng, Changjiang Fan, Xiangcheng Zheng and Tianheng Zhao
Water 2025, 17(12), 1720; https://doi.org/10.3390/w17121720 - 6 Jun 2025
Abstract
Plant roots and soil properties play crucial roles in regulating soil hydrological processes, particularly in determining soil water infiltration capacity. However, the infiltration patterns and underlying mechanisms across different forest types in subtropical regions remain poorly understood. In this study, we measured the [...] Read more.
Plant roots and soil properties play crucial roles in regulating soil hydrological processes, particularly in determining soil water infiltration capacity. However, the infiltration patterns and underlying mechanisms across different forest types in subtropical regions remain poorly understood. In this study, we measured the infiltration characteristics of three typical stands (pure Phyllostachys edulis forest, mixed Phyllostachys edulis-Cunninghamia lanceolata forest, and pure Cunninghamia lanceolata forest) using a double-ring infiltrometer. Stepwise multiple regression and structural equation modeling (SEM) were employed to analyze the effects of root traits and soil physicochemical properties on soil infiltration capacity. The results revealed the following: (1) The initial infiltration rate (IIR), stable infiltration rate (SIR), and average infiltration rate (AIR) followed the order pure Phyllostachys edulis stand > mixed stand > pure Cunninghamia lanceolata stand. (2) Compared to the pure Cunninghamia lanceolata stand, the IIR, SIR, and AIR in the pure Phyllostachys edulis stand increased by 6.66%, 35.63%, and 28.51%, respectively, while those in the mixed stand increased by 28.79%, 28.82%, and 33.51%. (3) Fine root biomass, root length density, non-capillary porosity, and soil bulk density were identified as key factors influencing soil infiltration capacity. (4) Root biomass and root length density affected infiltration capacity through both direct pathways and indirect pathways mediated by alterations in non-capillary porosity and soil bulk density. These findings provide theoretical insights into soil responses to forest types and inform sustainable water–soil management practices in Phyllostachys edulis plantations. Full article
(This article belongs to the Section Hydrology)
17 pages, 1842 KiB  
Article
Impact of Vegetal Protein on the Physicochemical and Microstructural Properties of Microencapsulated Mexican Red Pitaya (Stenocereus thurberi) Juice
by David Neder-Suárez, Carmen Oralia Meléndez-Pizarro, Esther Pérez-Carrillo, Jesús Alberto Vázquez-Rodríguez, María del Cielo Valdez-Cárdenas, Martha Graciela Ruiz-Gutiérrez, Carlos Abel Amaya-Guerra, Francisco Paraguay-Delgado and Armando Quintero-Ramos
AppliedChem 2025, 5(2), 12; https://doi.org/10.3390/appliedchem5020012 - 6 Jun 2025
Abstract
The effects of different carrier agents—pea protein (PP), rice protein (RP), bean protein (BP), whey protein (WP), and maltodextrin (MT, as a control)—on pitaya juice encapsulation via spray drying were evaluated. Juice and carrier mixtures (30% w/v) were dried at [...] Read more.
The effects of different carrier agents—pea protein (PP), rice protein (RP), bean protein (BP), whey protein (WP), and maltodextrin (MT, as a control)—on pitaya juice encapsulation via spray drying were evaluated. Juice and carrier mixtures (30% w/v) were dried at 150 °C, and the resulting powders were analyzed for water activity (aw), hygroscopicity (Hg), water solubility (WSI), bulk density (BD), glass transition temperature (Tg), water absorption (WAI), antioxidant activity (AA), total polyphenol content (TPC), total betalain (TB) content, and TB stability. Vegetable proteins showed promising results, significantly impacting the protein content, Hg content, WAI, WSI, AA, TPC, and TB content and resulting in high Tg values. PP showed the best results, with high betalain retention (>30%), high TPC and AA, high protein levels, and low Hg, similarly to MT. WP had the highest TB, AA, and TPC but the lowest Tg (47.21 °C), thus reducing stability. Encapsulates obtained with plant protein-based wall materials presented high Tg (>58 °C); low aw, WSI, and Hg; high protein contents >40%; and adequate retention of bioactive compounds, with low degradation rate constants and long half-lives. Overall, plant proteins are promising alternatives to traditional carriers, offering improved stability and functionality in encapsulated products. Full article
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13 pages, 2357 KiB  
Article
Effect of Coal Gangue Powder Addition on Hydraulic Properties of Aeolian Sandy Soil and Plant Growth
by Xiaoyun Ding, Ruimin He, Zhenguo Xing, Haoyan Wei, Jiping Niu, Shi Chen and Min Li
Horticulturae 2025, 11(6), 634; https://doi.org/10.3390/horticulturae11060634 - 5 Jun 2025
Abstract
Coal gangue is a fine-grained mineral with nutrient content, which can be used as a potential soil amendment. Nevertheless, current research on using coal gangue to improve soil water and support plant growth is still insufficient. In this study, coal gangue powder (CGP) [...] Read more.
Coal gangue is a fine-grained mineral with nutrient content, which can be used as a potential soil amendment. Nevertheless, current research on using coal gangue to improve soil water and support plant growth is still insufficient. In this study, coal gangue powder (CGP) was added to aeolian sandy soil. We compared the soil hydraulic properties and plant growth of original aeolian sandy soil (CK) and different CGP application rates (10% and 20%). The results indicated that the application of CGP transformed the soil texture from sandy to loamy, significantly reduced soil bulk density and saturated hydraulic conductivity (Ks) values, altered the soil water characteristic curve, enhanced soil water-holding capacity, and increased plant-available water. Compared with the CK group, the emergence rate of alfalfa seeds increased from approximately 50% to over 70% after CGP application. During the growth process, CGP application significantly elevated the net photosynthetic rate, transpiration rate, and stomatal conductance of alfalfa leaves. Rapid fluorescence kinetics monitoring of leaves demonstrated that alfalfa treated with CGP had a higher efficiency in light energy utilization. However, the photosynthetic capacity of leaves did not improve as the CGP application rate increased from 10% to 20%, suggesting that excessive CGP addition did not continuously benefit plant gas exchange. In conclusion, CGP application can improve the soil hydraulic properties of aeolian sandy soil and support plant growth and development, which is conducive to reducing the accumulated amount of coal gangue, alleviating plant water stress, and promoting ecological restoration in arid mining areas. We recommend a 10% addition of coal gangue powder as the optimal amount for similar soils. Full article
(This article belongs to the Section Plant Nutrition)
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19 pages, 2614 KiB  
Article
Influence of Microclimatic Variations on Morphological Traits of Ferns in Urban Forests of Central Veracruz, Mexico
by Jessica G. Landeros-López, Thorsten Krömer, Jorge A. Gómez-Díaz, Noé Velázquez-Rosas and César I. Carvajal-Hernández
Plants 2025, 14(11), 1732; https://doi.org/10.3390/plants14111732 - 5 Jun 2025
Abstract
Urban forests are remnants of forest habitats within urban areas. Their structural alterations create stressful microclimatic conditions that can influence the morphology of sensitive plants, such as ferns. This study analyzed variations in the morphological traits of ferns in four urban forest sites [...] Read more.
Urban forests are remnants of forest habitats within urban areas. Their structural alterations create stressful microclimatic conditions that can influence the morphology of sensitive plants, such as ferns. This study analyzed variations in the morphological traits of ferns in four urban forest sites in central Veracruz, Mexico, considering the microclimatic differences arising from vegetation structure. Temperature, humidity, canopy openness, and radiation were measured, along with eight foliar traits, while assessing the impact of site and habit (terrestrial or epiphytic) on the response. Sites with greater alterations in vegetation structure exhibited increased canopy openness, solar radiation, temperature, and a higher number of days with lower relative humidity. In these sites, leaves showed an increase in dry matter content and vein density, indicating a greater investment in resource storage and structural resistance. In the less-disturbed sites, terrestrial ferns demonstrated larger leaf area and specific leaf area, suggesting greater growth potential. Conversely, epiphytes generally had smaller leaves, which could represent an adaptive advantage for these species. The results also suggest a process of biotic homogenization within this plant group, reflecting a similar morphological response, except for indicator species restricted to less disturbed sites. Thus, this study reveals that microclimatic variations induced by urbanization significantly affect plant morphology and, ultimately, species diversity. Full article
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16 pages, 2003 KiB  
Article
Mycelium-Based Composites Derived from Lignocellulosic Residual By-Products: An Insight into Their Physico-Mechanical Properties and Biodegradation Profile
by Galena Angelova, Husein Yemendzhiev, Roumiana Zaharieva, Mariya Brazkova, Ralitza Koleva, Petya Stefanova, Radka Baldzhieva, Veselin Vladev and Albert Krastanov
Appl. Sci. 2025, 15(11), 6333; https://doi.org/10.3390/app15116333 - 5 Jun 2025
Abstract
The bio-fabrication of sustainable mycelium-based composites (MBCs) from renewable plant by-products offers a promising approach to reducing resource depletion and supporting the transition to a circular economy. In this research, MBCs were obtained by cultivating Ganoderma resinaceum GA1M on essential oils and agricultural [...] Read more.
The bio-fabrication of sustainable mycelium-based composites (MBCs) from renewable plant by-products offers a promising approach to reducing resource depletion and supporting the transition to a circular economy. In this research, MBCs were obtained by cultivating Ganoderma resinaceum GA1M on essential oils and agricultural by-products: hexane-extracted rose flowers (HERF), steam-distilled lavender straw (SDLS), wheat straw (WS), and pine sawdust (PS), used as single or mixed substrates. The basic physical and mechanical properties revealed that MBCs perform comparably to high-efficiency thermal insulating and conventional construction materials. The relatively low apparent density, ranging from 110 kg/m3 for WS-based to 250 kg/m3 for HERF-based composites, results in thermal conductivity values between 0.043 W/mK and 0.054 W/mK. Compression stress (40–180 kPa at 10% deformation) also revealed the good performance of the composites. The MBCs had high water absorption due to open porosity, necessitating further optimization to reduce hydrophilicity and meet intended use requirements. An aerobic biodegradation test using respirometry indicated ongoing microbial decomposition for all tested bio-composites. Notably, composites from mixed HERF and WS (50:50) showed the most rapid degradation, achieving over 46% of theoretical oxygen consumption for complete mineralization. The practical applications of MBCs depend on achieving a balance between biodegradability and stability. Full article
(This article belongs to the Section Materials Science and Engineering)
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25 pages, 12268 KiB  
Article
Modeling Growth Dynamics of Lemna minor: Process Optimization Considering the Influence of Plant Density and Light Intensity
by Jannis von Salzen, Finn Petersen, Andreas Ulbrich and Stefan Streif
Plants 2025, 14(11), 1722; https://doi.org/10.3390/plants14111722 - 5 Jun 2025
Abstract
The production of duckweed (Lemnaceae) as a novel protein source could make a valuable contribution to human nutrition. The greatly reduced habitus of duckweed enables simple cultivation with extremely low space requirements, making this free-floating freshwater plant ideal for substrate-free and vertical cultivation [...] Read more.
The production of duckweed (Lemnaceae) as a novel protein source could make a valuable contribution to human nutrition. The greatly reduced habitus of duckweed enables simple cultivation with extremely low space requirements, making this free-floating freshwater plant ideal for substrate-free and vertical cultivation in controlled environment agriculture. Of particular importance in the design of a plant-producing Indoor Vertical Farming process is the determination of light intensity, as artificial lighting is generally the most energy-intensive feature of daylight-independent cultivation systems. In order to make the production process both cost-effective and low emission in the future, it is, therefore, crucial to understand and mathematically describe the primary metabolism, in particular the light utilization efficiency. To achieve this, a growth model was developed that mathematically describes the combined effects of plant density and light intensity on the growth rate of Lemna minor L. and physiologically explains the intraspecific competition of plants for light through mutual shading. Furthermore, the growth model can be utilized to derive environmental and process parameters, including optimum harvest quantities and efficiency-optimized light intensities to improve the production process. Full article
(This article belongs to the Special Issue Duckweed: Research Meets Applications—2nd Edition)
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16 pages, 1946 KiB  
Article
Spodoptera frugiperda Uses Specific Volatiles to Assess Maize Development for Optimal Offspring Survival
by Hanbing Li, Peng Wan, Zhihui Zhu, Dong Xu, Shengbo Cong, Min Xu and Haichen Yin
Insects 2025, 16(6), 592; https://doi.org/10.3390/insects16060592 - 4 Jun 2025
Viewed by 13
Abstract
Spodoptera frugiperda, a major global agricultural pest, poses significant challenges to chemical control methods due to pesticide resistance and environmental concerns, underscoring the need for sustainable management strategies. Attractants based on host plant volatiles offer a promising eco-friendly approach, but their development [...] Read more.
Spodoptera frugiperda, a major global agricultural pest, poses significant challenges to chemical control methods due to pesticide resistance and environmental concerns, underscoring the need for sustainable management strategies. Attractants based on host plant volatiles offer a promising eco-friendly approach, but their development for S. frugiperda is hindered by limited research on host recognition mechanisms. This study reveals that female S. frugiperda preferentially oviposit on maize at the seedling stage. Using electrophysiological techniques, we identified p-xylene and (+)-camphor from seedling-stage maize volatiles as key compounds eliciting strong responses in female S. frugiperda. Behavioral assays confirmed that these compounds (p-xylene at the concentration of 5%, 10%, and 20% and (+)-camphor at 1%, 5%, and 10%) significantly attract females, establishing them as the key odor cues for host selection. Moreover, these volatiles are more abundant in seedling-stage maize, suggesting that S. frugiperda assesses maize growth stages based on their concentrations. Importantly, larvae reared on seedling-stage maize exhibited higher survival rates than those on later-stage maize, indicating that oviposition site selection directly affects offspring fitness. These findings demonstrate that S. frugiperda uses p-xylene and (+)-camphor to evaluate maize development and select suitable oviposition sites, thereby enhancing larval survival. This study provides a foundation for developing targeted attractants for S. frugiperda and highlights the seedling stage as a critical period for implementing pest control measures, particularly in autumn maize production, given the higher pest population density during this phase. Full article
(This article belongs to the Section Insect Behavior and Pathology)
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24 pages, 5633 KiB  
Article
Architectural Analysis for Novel Olive Crop Management
by Khouloud Annabi, Faouzi Haouala, AbdelKarim Hamrita, Rania Kouki, Foued Laabidi, Mokhtar Rejili, Samra Akef Bziouech and Mouna Mezghani Aïachi
Plants 2025, 14(11), 1707; https://doi.org/10.3390/plants14111707 - 3 Jun 2025
Viewed by 129
Abstract
Efficient fruit production, quality improvement, and timely harvesting are essential in olive cultivation, which requires optimised distribution and management of fruiting sites. This study aimed to support sustainable olive crop management by analysing the morphological characteristics of five cultivars (Chemlali, Chetoui [...] Read more.
Efficient fruit production, quality improvement, and timely harvesting are essential in olive cultivation, which requires optimised distribution and management of fruiting sites. This study aimed to support sustainable olive crop management by analysing the morphological characteristics of five cultivars (Chemlali, Chetoui, Koroneiki, Meski, and Picholine) under semi-arid Tunisian conditions. Through a detailed architectural analysis, we investigated the relationships between branching patterns, density, distribution of inflorescence and fruit sites, biometric traits (shoot length, internode number, and shoot dimensions), and geometric variability within each cultivar. Three trees per cultivar were analysed across three architectural units. The results showed marked architectural differences, highlighting the need for cultivar-specific strategies in planting, pruning, and orchard management. The distribution of shoots across botanical orders revealed unique branching patterns: Chemlali and Koroneiki showed thinner shoots and higher shoot density, reflecting strong apical dominance and their suitability for hyper-intensive systems. In addition, nonsignificant differences in long shoots’ insertion angles between Meski, Chetoui, and Koroneiki suggest compatibility for co-cultivation, facilitating mechanised maintenance and harvesting. Emphasis on inter-cultivar compatibility and architectural coherence is crucial for orchard design. These findings provide important insights for optimising orchard management practices to improve productivity, fruit quality, and operational efficiency. Full article
(This article belongs to the Special Issue Development of Woody Plants)
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17 pages, 2188 KiB  
Article
Employment of Biodegradable, Short-Life Mulching Film on High-Density Cropping Lettuce in a Mediterranean Environment: Potentials and Prospects
by Marco Pittarello, Maria Teresa Rodinò, Rossana Sidari, Maria Rosaria Panuccio, Francesca Cozzi, Valentino Branca, Beatrix Petrovičová and Antonio Gelsomino
Agriculture 2025, 15(11), 1219; https://doi.org/10.3390/agriculture15111219 - 3 Jun 2025
Viewed by 92
Abstract
Biodegradable mulch films were developed over the last decades to replace polyethylene, but their short durability and higher costs still limit their diffusion. This work aimed to test an innovative composite mulching film constituted by a mixture of carboxylmethyl cellulose, chitosan and sodium [...] Read more.
Biodegradable mulch films were developed over the last decades to replace polyethylene, but their short durability and higher costs still limit their diffusion. This work aimed to test an innovative composite mulching film constituted by a mixture of carboxylmethyl cellulose, chitosan and sodium alginate, enriched or not with an inorganic N- and P-source to help the microbial breakdown in soil. The trial was carried out using outdoor mesocosms cultivated with lettuce plants with high-density planting. Commercial Mater-Bi® and a polyethylene film were taken as control treatments. Air temperature and humidity monitored daily during the 51 d cropping cycle remained within the ideal range for lettuce growth with no mildew or fungi infection. Visible mechanical degradation of the experimental biopolymers occurred after 3 weeks; however, Mater-Bi® and polyethylene remained unaltered until harvest. Chemical soil variables (TOC, TN, CEC, EC) remained unchanged in all theses, whereas the pH varied. The yield, pigments, total phenols, flavonoids and ROS scavenging activity of lettuce were similar among treatments. Despite their shorter life service (~3 weeks), polysaccharide-based mulching films showed their potential to protect lettuce plants at an early stage and provide yield and nutraceutical values similar to conventionally mulched plants, while allowing a reduced environmental impact and disposal operations. Full article
(This article belongs to the Section Crop Production)
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21 pages, 6822 KiB  
Article
Soil Physicochemical Improvement in Coastal Saline–Alkali Lands Through Salix matsudana × alba Plantation
by Zhenxiao Chen, Zhenan Chen and Handong Gao
Forests 2025, 16(6), 933; https://doi.org/10.3390/f16060933 - 2 Jun 2025
Viewed by 115
Abstract
To evaluate the ecological remediation effect of Salix matsudana × alba on saline coastal soils, we established a five-year field experiment in Rudong County, Jiangsu Province, China. The experiment was designed with three salinity gradients (low, medium, and high) and five plant spacing [...] Read more.
To evaluate the ecological remediation effect of Salix matsudana × alba on saline coastal soils, we established a five-year field experiment in Rudong County, Jiangsu Province, China. The experiment was designed with three salinity gradients (low, medium, and high) and five plant spacing treatments (2 × 2 m, 2 × 3 m, 3 × 3 m, 3 × 4 m, and 4 × 4 m). Soil samples were collected annually at a depth of 0–20 cm using grid and random sampling methods. Indicators of soil physicochemical properties and heavy metal content were measured, including soil organic matter (SOM), pH, total nitrogen (TN), total phosphorus (TP), total potassium (TK), electrical conductivity (EC), total salinity (TS), and bulk density (BD). Additionally, eight heavy metals were analyzed: zinc (Zn), chromium (Cr), nickel (Ni), copper (Cu), cadmium (Cd), lead (Pb), arsenic (As), and mercury (Hg). Results showed that the hybrid willow significantly improved SOM content by up to 90% and reduced EC and TS by 52% and 60% over five years, especially under low and medium salinity conditions with dense planting (2 × 2 m, 2 × 3 m). The content of most heavy metals exhibited a decreasing trend or remained stable, indicating the plant’s phytostabilization potential (i.e., stabilization of heavy metals via plant-soil interaction). Principal component analysis (PCA) and random forest (RF) modeling identified SOM, EC, TS, and BD as the dominant factors influencing soil quality improvement. A soil quality index (SQI) was constructed based on PCA-derived weights, which further confirmed the positive ecological effect of this hybrid species on coastal saline soils. This study provides scientific evidence supporting the use of Salix matsudana × alba as a promising species for large-scale ecological restoration in coastal saline-alkaline lands. Full article
(This article belongs to the Section Forest Soil)
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15 pages, 2026 KiB  
Article
SODS: Soil Health On-Demand Sensors—A Multi Parameter Field Study with Temporal Monitoring
by Vikram Narayanan Dhamu, Mohammed A. Eldeeb, Anil C. Somenahally, Sriram Muthukumar and Shalini Prasad
Sensors 2025, 25(11), 3505; https://doi.org/10.3390/s25113505 - 1 Jun 2025
Viewed by 419
Abstract
Real-time monitoring of soil health parameters is crucial for efficient use of resources, improving agricultural productivity, and sustainability. Traditional soil analysis methods, although accurate, are time-consuming and lack the spatial and temporal resolution necessary for dynamic agricultural environments. Recent advancements in sensor technology [...] Read more.
Real-time monitoring of soil health parameters is crucial for efficient use of resources, improving agricultural productivity, and sustainability. Traditional soil analysis methods, although accurate, are time-consuming and lack the spatial and temporal resolution necessary for dynamic agricultural environments. Recent advancements in sensor technology offer promising alternatives, enabling real-time, in situ monitoring of key soil health indicators. This study details the deployment and validation of novel Sensor-in-Field probes at the Donald Danforth Plant Science Center Farm in Missouri, U.S., in a winter wheat plot. Three Sensor-in-Field probes were evaluated for their ability to measure nitrate (NO3), ammonium (NH4), soil organic matter (SOM), carbonaceous soil minerals (CSMs), soil volumetric density (SVD), soil hydration state (SHS), and total soil carbon (TSC) over a 28-day period. The probes’ coefficients of variation were well within acceptable limits (<20%) for all parameters. The measured metrics averaged 0.05% ± 0.001 and 1.92% ± 0.02 for CSMs and SOM, respectively, while TSC was 1.18% ± 0.15. For the nutrients, the measured NO3 and NH4 values were 4.44 ppm ± 0.37 and 2.78 ppm ± 0.22, respectively. The accuracy of the soil probes was validated at a certified traditional soil analysis laboratory. Three samples were collected at three different time points and analyzed. Bland–Altman analysis showed <± 10% difference between the soil probes and traditional lab analysis for CSMs, SOM, and TSC, while t-test analysis reported p-values > 0.005 for NO3, NH4, and SHS/SVD, indicating non-significant differences between the probes and traditional soil analysis methods. Full article
(This article belongs to the Section Intelligent Sensors)
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11 pages, 1597 KiB  
Article
Forestry Assisted Migration in a Longleaf Pine Ecosystem
by Avery S. Holbrook and Joshua J. Puhlick
Forests 2025, 16(6), 932; https://doi.org/10.3390/f16060932 - 1 Jun 2025
Viewed by 171
Abstract
Case studies of climate adaptation approaches are needed to inform the broader use of these strategies across longleaf pine (Pinus palustris Mill.) ecosystems in the Southern US. To address this need, we evaluated overstory structure and tree regeneration in longleaf pine-hardwood stands, [...] Read more.
Case studies of climate adaptation approaches are needed to inform the broader use of these strategies across longleaf pine (Pinus palustris Mill.) ecosystems in the Southern US. To address this need, we evaluated overstory structure and tree regeneration in longleaf pine-hardwood stands, and a transition approach was implemented to intentionally facilitate change to encourage adaptive responses. Stand density reduction and species selection were prescribed to reduce tree vulnerability to drought stress. Turkey oaks (Quercus laevis W.) were also planted as a part of an assisted population migration strategy. After the treatments, Hurricane Michael impacted the study stands. The percent reduction in large overstory longleaf pines due to the hurricane was 6.3 ± 6.1% (grand mean ± standard deviation, which was derived from the stand means). At least one live planted turkey oak was present in 74 ± 26% of the planted clusters of turkey oaks that could be located six years after planting them. Our findings demonstrate the ability of transition stands to accommodate a large-scale disturbance event and maintain ecosystem functionality, the desired stand structure, and species composition. The relative success of forestry assisted migration plantings of turkey oaks may alleviate some concerns about the risk of maladaptation. Full article
(This article belongs to the Section Forest Ecology and Management)
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15 pages, 3937 KiB  
Article
Genome-Wide Identification of SNP and SSR Markers from Cymbidium goeringii and C. faberi for Their Potential Application in Breeding
by Mengya Cheng, Yingqi Liu, Chentai Jin, Xiao Jiang, Xiuming Chen, Fei Wang, Yanru Duan, Xiaokang Zhuo and Donghui Peng
Horticulturae 2025, 11(6), 622; https://doi.org/10.3390/horticulturae11060622 - 1 Jun 2025
Viewed by 157
Abstract
Chinese Cymbidium are prized for their ornamental beauty, ecological significance, and economic value. However, genomic resources crucial for breeding studies within this genus remain scarce, which has hindered the identification of key genes controlling economically important traits and posed challenges for conservation efforts. [...] Read more.
Chinese Cymbidium are prized for their ornamental beauty, ecological significance, and economic value. However, genomic resources crucial for breeding studies within this genus remain scarce, which has hindered the identification of key genes controlling economically important traits and posed challenges for conservation efforts. We performed a comprehensive identification of whole-genome simple sequence repeats (SSRs) and single-nucleotide polymorphism (SNP) markers using the restriction-site associated DNA sequencing (RADseq) on C. goeringii and C. faberi. A total of 49,640 SSR loci were identified across both species, with an average density of 12.7 SSRs/Mb. Among these, 17,637 SSRs were common to both C. goeringii and C. faber, while 17,676 and 14,329 SSRs were uniquely identified in C. goeringii and C. faberi, respectively. Additionally, we identified 405,416 SNPs and 26,870 InDels, with average densities of 105.2/Mb and 6.5/Mb. Furthermore, we validated two SSRs (located at Chr01:78857480-78860461 and Chr01:93382182-93384869) and developed an efficient method for identifying hybrids among the progeny resulting from crosses between C. goeringii and C. faberi. We also validated two SNP markers that showed a close association with the petal and lip length using Sanger sequencing. Our findings revealed that the Chr01_99657375 SNP achieved 73% predictive accuracy for identifying long-petal/lip phenotypes. The results are expected to greatly benefit marker-assisted breeding efforts in Cymbidium orchids and lay a solid foundation for the molecular breeding process of improving flower shape traits in orchid plants. Full article
(This article belongs to the Special Issue Orchids: Advances in Propagation, Cultivation and Breeding)
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23 pages, 7170 KiB  
Article
Vegetation Configuration Effects on Microclimate and PM2.5 Concentrations: A Case Study of High-Rise Residential Complexes in Northern China
by Lina Yang, Xu Li, Daranee Jareemit and Jiying Liu
Atmosphere 2025, 16(6), 672; https://doi.org/10.3390/atmos16060672 - 1 Jun 2025
Viewed by 204
Abstract
While urban greenery is known to regulate microclimates and reduce air pollution, its integrated effects remain insufficiently quantified. Through field monitoring and ENVI-met 5.1 modeling of high-rise residential areas in Jinan, the results demonstrate that: (1) vegetation exhibits distinct spatial impacts in air-quality [...] Read more.
While urban greenery is known to regulate microclimates and reduce air pollution, its integrated effects remain insufficiently quantified. Through field monitoring and ENVI-met 5.1 modeling of high-rise residential areas in Jinan, the results demonstrate that: (1) vegetation exhibits distinct spatial impacts in air-quality impacts, reducing roadside PM2.5 by 26.63 μg/m3 while increasing building-adjacent levels by 17.5 μg/m3; (2) shrubs outperformed trees in PM2.5 reduction (up to 65.34%), particularly when planted in inner rows, whereas tree crown morphology and spacing showed negligible effects; (3) densely spaced columnar trees optimize cooling, reducing Ta by 3–4.8 °C and the physiological equivalent temperature (PET*) by 8–12.8 °C, while planting trees on the outer row and shrubs on the inner row best balanced thermal and air-quality improvements; (4) each 1 m2/m3 leaf area density (LAD) increase yields thermal benefits (ΔTa = −1.07 °C, ΔPET* = −1.93 °C) but elevates PM2.5 by 4.32 μg/m3. These findings provide evidence-based vegetation design strategies for sustainable urban planning. Full article
(This article belongs to the Section Air Quality)
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19 pages, 8136 KiB  
Article
Nitrogen Fertilizer Application and Optimized Planting Density Enhance Rice Yield by Improving the Panicle Type Index and Increasing the Filling Rate of Inferior Grains
by Yanlong Gong, Yue Lei, Zhongni Wang, Hai Xu, Xiaoyi Cheng and Wenfu Chen
Plants 2025, 14(11), 1690; https://doi.org/10.3390/plants14111690 - 31 May 2025
Viewed by 271
Abstract
This study aimed to investigate the regulatory effects of nitrogen (N) application rate and plant density on panicle type index (PTI), yield, grain filling characteristics, and their correlations. The low-PTI rice variety DP128 (PTI = 0.15) was cultivated under field conditions at four [...] Read more.
This study aimed to investigate the regulatory effects of nitrogen (N) application rate and plant density on panicle type index (PTI), yield, grain filling characteristics, and their correlations. The low-PTI rice variety DP128 (PTI = 0.15) was cultivated under field conditions at four N supply levels (0 (N0), 140 (N140), 200 (N200), and 260 (N260) kg∙ha–1), and two plant densities (166,755 and 333,495 plants∙ha−1). Results showed that N application rate, planting density, and their interactions significantly influenced yield, PTI, grain number in middle/lower secondary branches, and total grain number in lower secondary branches of rice DP128. Parameters trends were consistent over two years. Under N200D10, the total grain number in lower secondary branches was minimized, while other indices were maximized. Further analysis indicates that under high PTI conditions, the maximum grain-filling rate (Gmax), mean grain-filling rate (Gmean), sucrose content, ABA/ETH ratio, and starch content in inferior grains (IGs) were all significantly elevated. Correlation analysis indicated PTI was positively correlated with yield, grain number in middle/lower secondary branches, IGs−Gmax, and IGs−Gmean and negatively correlated with the total grain number in the lower secondary branches. In summary, increasing PTI can be achieved by optimizing the distribution of secondary branch grains along the panicle axis, decreasing the number of grains on the lower secondary branches, mitigating the competition for filling materials among inferior grains, improving grain-filling capacity and, ultimately, increasing rice yield. Full article
(This article belongs to the Section Crop Physiology and Crop Production)
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