Search Results (2,175)

Floods seriously threaten public health by promoting the spread of antimicrobial-resistant (AMR) bacteria, particularly in urban areas with poor sanitation. In May 2024, the state of Rio Grande do Sul, Brazil, experienced the most severe flood in its history, affecting over 2.3 million people and resulting in extensive dissemination of sewage, contaminating the environment. This study aimed to investigate the presence of Enterobacteriaceae and clinically relevant carbapenemase genes (bla_KPC and bla_NDM) in floodwaters from Porto Alegre using molecular methods. Seventy-nine water samples were collected during four sampling campaigns conducted between May and June 2024. Samples were obtained from flooded areas and points across Guaíba Lake. DNA was extracted with the DNeasy PowerWater Kit, and qPCR was performed using TaqMan assays targeting Enterobacteriaceae, bla_KPC and bla_NDM. Of the 79 samples, 75 yielded sufficient DNA for analysis. Enterobacteriaceae were detected in 100% of the samples, across all collections. The bla_KPC gene was detected in 100% of the first collection pools, and in 94.7%, 94.7%, and 85.7% of samples from the second, third, and fourth collections, respectively. The bla_NDM gene was present in 81.3% of the first collection pools, and in 78.9%, 89.4%, and 80.9% of samples from the subsequent collections. The high prevalence of Enterobacteriaceae and carbapenemase genes in floodwaters reveals an alarming environmental dissemination of AMR genetic markers. These findings underscore the need for environmental AMR surveillance, especially in disaster settings, and support the implementation of the One Health approach to mitigate the spread of resistance genes across human, animal, and environmental interfaces. Full article

This study investigates the environmental dimension of sustainable development across fifteen post-Soviet republics in 2022. While sustainability is generally understood as a triadic construct—economic, social, and environmental—this paper isolates the ecological pillar to highlight cross-country differences shaped by industrial legacies, institutional capacity, and governance models. A composite Environmental Performance Index (EPI) is developed using the Mazziotta–Pareto Index (MPI), which captures both average performance and internal consistency across three SDG-related domains: SDG 6 (Clean Water and Sanitation), SDG 13 (Climate Action), and SDG 15 (Life on Land). The study adds to existing literature as it includes a non-compensatory composite index and cluster analysis, and in policy terms, it provides a benchmarking system for facilitating ecological transition in the post-Soviet context. The results reveal strong divergence across the region: Baltic countries and Moldova achieve higher scores, reflecting policy convergence with the European Union and stronger environmental institutions, while Central Asian republics lag due to resource dependence, water scarcity, and weaker governance. Geographic cluster analysis corroborates these differences, showing clear spatial patterns of environmental convergence and divergence. Correlation analysis further demonstrates that environmental sustainability is positively associated with GDP per capita, HDI, and life expectancy, while negatively linked with inequality and fertility rates. These findings stress the need for context-sensitive and evidence-based policies, intra-regional cooperation, and integrated governance mechanisms to advance ecological transition in line with the 2030 Agenda for Sustainable Development. Full article

Methicillin-resistant Staphylococcus spp. are microorganisms found in dairy products, bovine mastitis, and human infections. The prevalence of resistant strains from this genus in the food chain is increasing, drawing attention to transmission in the community and highlighting the importance of One Health studies. Thus, the aim of this study was to determine the MIC of oxacillin (OXA) and the sanitizers benzalkonium chloride (BAC) and sodium hypochlorite (HP) against isolates of methicillin-resistant Staphylococcus spp., and to evaluate the possible influence of sub-MIC application of these compounds on bacterial cells, in order to observe possible microbial resistance. Ten isolates of methicillin-resistant Staphylococcus spp. (S. epidermidis and S. chromogenes) were used. Among the sanitizers, BAC showed greater efficiency during the pre-inhibition test. Increased resistance to OXA was found in isolates of S. chromogenes and S. epidermidis after sub-MICs of 50% and 90% of OXA, while sub-inhibition of HP favored resistance to OXA. The application of HP and OXA, even at low concentrations, induced a reduction in biofilm production. This study shows that sub-inhibitory sanitizer exposure in Staphylococcus spp. induces antimicrobial resistance phenotypes linked to mutations in regulatory, mobile, and DNA repair genes. These findings suggest that selective pressure promotes resistant variants through genomic plasticity and regulatory activation, supporting the hypothesis that sanitizer residues may drive multidrug resistance emergence, although further functional validation is required. Full article

The emergence of Candida auris as a disheartening fungal pathogen in healthcare settings has prompted urgent re-evaluation of containment and mitigation strategies. This review critically examines the biological persistence, environmental adaptability, and resistance to standard antifungal therapies of the pathogen, particularly regions with limited surveillance infrastructure. Based on regional experiences, such as those in Saudi Arabia and the Middle East in general, the study reveals systemic weaknesses in diagnosis, reporting, and environmental sanitation. Special consideration is paid to the combination of new disinfection technologies, including ultraviolet irradiation systems and hydrogen peroxide vaporisation, with institutional behaviour change strategies. This discussion shows the importance of synchronising technological development with frequent employee contributions and cross-functional planning. It also encourages the international standardisation of diagnostic platforms and the launch of real-time genomic surveillance to reveal evolutionary trends. Finally, the findings justify the shift towards proactive models of infection control that are founded on the resilience of systems and the agility of institutions. This paper is a synthesis of epidemiological patterns, decontamination strategies and behavioural knowledge to contribute to an emerging body of knowledge that can help to fortify healthcare settings against current fungal threats. Full article

Background/Objectives: Extreme temperatures linked to climate change threaten child health, particularly in Sub-Saharan Africa where malnutrition remains widespread. This study examines how exposure to hot and cold temperature anomalies influences child stunting in Burkina Faso and Kenya and evaluates how household infrastructure and socio-demographic factors interact with climate stressors to shape outcomes. Methods: We combined nationally representative Demographic and Health Surveys (Burkina Faso 2021; Kenya 2022) with daily maximum and minimum temperature data from the Climate Hazards InfraRed Temperature with Stations (CHIRTS). The analytic sample included children aged 24–59 months. Temperature anomalies were calculated as standardized deviations from local historical averages. Multilevel logistic regression models assessed associations between stunting, climate anomalies, and household-level factors, including electricity, water, sanitation, wealth, and rural/urban residence. Results: Heat anomalies were linked to increased stunting risk in Kenya (β = 2.34, p < 0.001), while in Burkina Faso, higher maximum temperatures unexpectedly reduced stunting odds (β = 0.08, p < 0.05). Cold anomalies showed marginal positive associations with stunting in both countries. Infrastructure and socioeconomic factors varied by context: electricity access and urban residence were protective in Burkina Faso, while improved sanitation, household wealth, and child sex differences were significant in Kenya. Conclusions: Climate anomalies and household conditions jointly influence stunting among children aged 24–59 months, with effects varying by country. Cold anomalies were associated with higher odds of stunting in Burkina Faso (BF OR = 2.14) and Kenya (KE OR = 1.20), while heat anomalies reduced stunting in BF (OR = 0.08) but increased it in KE (OR = 2.34). Electricity access was protective in both countries (BF OR = 0.61; KE OR = 0.71), while improved water, sanitation, and wealth were significant only in KE. Older child age consistently reduced stunting risk, and urban residence was protective only in BF. These findings underscore that climate impacts on stunting are context-specific and highlight the need for policies integrating climate adaptation with investments. Full article

Municipal solid waste (MSW) refers to solid and semi-solid waste generated during human production and daily activities. The process of incinerating such waste, known as municipal solid waste incineration (MSWI), serves as a critical method for reducing waste volume and recovering resources. Automatic online recognition of flame combustion status during MSWI is a key technical approach to ensuring system stability, addressing issues such as high pollution emissions, severe equipment wear, and low operational efficiency. However, when manually selecting optimized features and hyperparameters based on empirical experience, the MSWI flame combustion state recognition model suffers from high time consumption, strong dependency on expertise, and difficulty in adaptively obtaining optimal solutions. To address these challenges, this article proposes a method for constructing a flame combustion state recognition model optimized based on reinforcement learning (RL), long short-term memory (LSTM), and parallel differential evolution (PDE) algorithms, achieving collaborative optimization of deep features and model hyperparameters. First, the feature selection and hyperparameter optimization problem of the ViT-IDFC combustion state recognition model is transformed into an encoding design and optimization problem for the PDE algorithm. Then, the mutation and selection factors of the PDE algorithm are used as modeling inputs for LSTM, which predicts the optimal hyperparameters based on PDE outputs. Next, during the PDE-based optimization of the ViT-IDFC model, a policy gradient reinforcement learning method is applied to determine the parameters of the LSTM model. Finally, the optimized combustion state recognition model is obtained by identifying the feature selection parameters and hyperparameters of the ViT-IDFC model. Test results based on an industrial image dataset demonstrate that the proposed optimization algorithm improves the recognition performance of both left and right grate recognition models, with the left grate achieving a 0.51% increase in recognition accuracy and the right grate a 0.74% increase. Full article

Breast cancer remains a leading cause of global cancer burden, with marked differences in incidence across countries. While biological risk factors are well established, understanding the broader structural and sociocultural influences has been less comprehensive. In this study, we analyzed harmonized data from 183 countries (2017–2023), encompassing 33 variables and 7 subvariables related to demographics, nutrition, environment, health, and healthcare access, drawn from open-access international databases. Spearman correlation analysis identified strong positive associations between breast cancer incidence and discontinued breastfeeding, high LDL cholesterol, out-of-pocket healthcare expenditure, and educational attainment. Conversely, poor sanitation, lack of handwashing facilities, unsafe water, and certain nutritional deficiencies exhibited robust negative correlations, likely reflecting under detection and reporting limitations in lower-resource settings rather than true protective effects. These findings were further explored using multiple linear regression, which explained approximately 73% of the variance in global breast cancer incidence. The final model highlighted discontinued breastfeeding, prevalence of cocaine use, unsafe sanitation, high out-of-pocket healthcare expenditure, limited handwashing access, and high processed meat consumption as the most influential independent predictors. Receiver operating characteristic (ROC) analysis confirmed strong predictive value for discontinued breastfeeding and out-of-pocket expenditure, with sanitation and hygiene variables showing paradoxical inverse associations. Our results emphasize that breast cancer risk is shaped not only by individual behaviors and genetics, but also by larger-scale structural, socioeconomic, and environmental factors. These patterns suggest that targeted interventions addressing both lifestyle behaviors and systemic inequities—such as promoting breastfeeding, reducing financial barriers to healthcare, and strengthening public health infrastructure—could meaningfully reduce the global burden of breast cancer. In conclusion, this study underscores the importance of multisectoral, equity-focused prevention strategies. It also highlights the value of country-level ecological analyses in uncovering upstream determinants of cancer incidence and calls for further research to disentangle individual and contextual effects in cancer epidemiology. Full article

Nano-sanitizers, which exploit the unique physicochemical properties of nanomaterials, are being increasingly investigated as innovative tools to promote food safety. In this argumentative review, we compare and contrast nano-sanitizers with conventional sanitation methods by examining their underlying antimicrobial mechanisms, multifaceted benefits, inherent challenges, and wide-ranging public health implications. We evaluate regulatory conundrums and consumer perspectives alongside future outlooks for integration with advanced technologies such as artificial intelligence. Through selective synthesis of the published literature, our argumentative discussion demonstrates that nano-sanitizers not only promise superior performance in pathogen inactivation but could also contribute to overall food system sustainability, provided safety and regulatory concerns are adequately addressed. Full article

Foodborne illness outbreaks from fresh produce underscore the urgent demand for sanitizing strategies that ensure safety while minimizing harmful by-products from high-dose chemical disinfectants such as sodium hypochlorite (NaOCl). Low-concentration combinations of organic acids and washing sanitizers were systematically evaluated to identify synergistic antibacterial effects, and citric acid (CA) was found to markedly potentiate the activity of NaOCl against Salmonella Typhimurium through a sequential assault on the cell envelope. A low-dose combination of sub-inhibitory concentrations (1/2 MIC of CA and 1/4 MIC of NaOCl) exhibited robust synergy, achieving a >6 log CFU/cm² reduction in the pathogen on a cherry tomato model within 3 min. Moreover, this synergistic entry leads to profound disruption of membrane integrity, resulting in leakage of nucleic acids and proteins, extensive oxidative damage, hyperpolarization, and cell lysis, as confirmed by electron and confocal microscopy together with physicochemical assays. Mechanistic investigation revealed that oxidative damage from NaOCl amplified CA-induced membrane acidification and permeability, facilitating deeper sanitizer penetration and accelerating envelope destruction. Collectively, these findings uncover a membrane-targeted synergistic mechanism, providing a solid scientific basis for the development of novel, low-residue, and high-efficacy food safety interventions. Full article

Foodborne diseases (FBDs) represent significant public health concerns as they are conditions associated with deficient manufacturing practices. They comprise important diseases with acute or chronic courses, frequently occurring in outbreak form and associated with significant gastrointestinal disorders. FBDs are related to infrastructure and organizational issues in urban centers, such that contamination in food processing facilities, lack of access to basic sanitation, and social and financial vulnerability are some of the factors that favor their occurrence and the demand for health services. Among the agents associated with FBDs is Listeria sp., especially Listeria monocytogenes (L. monocytogenes). The objective of this article is to characterize L. monocytogenes and its potential impact on One Health, given its importance as a significant foodborne pathogen. A thorough scientific literature search was conducted to obtain information on the subject, aiming to assist in the verification and presentation of evidence. L. monocytogenes is a pathogen with specific characteristics that ensure its adhesion, adaptation, growth, and survival on various surfaces, such as biofilm formation ability and thermotolerance. Several diagnostic methods are available for detection of the agent, including enrichment media, molecular techniques, and subtyping evaluation. Its control represents a significant challenge, with critical implications due to bacterial perpetuation characteristics and the implementation/monitoring of sanitization programs and commercialization of animal-derived products (POAO). Thus, vulnerable and susceptible populations are more exposed to foodborne pathogens due to health-related determinants, such as inadequate sanitation, poor food safety control, and insufficient personal hygiene. The pathogen’s persistence and difficulty of control represent a significant public One Health threat. Full article

Public events serve as a foundational mechanism for shaping the social and spatial dynamics of urban environments. Despite widespread recognition of their physical, psychological, and social impacts at the city scale, a significant gap persists in research addressing the social and spatial suitability of public spaces at the neighborhood level, particularly within the Arab urban context. This study investigates residential neighborhoods in Riyadh, Saudi Arabia, to assess how public events foster community engagement, cultural diversity, and social cohesion. Drawing on survey data from 510 residents, statistical analysis reveals that demographic variables such as age, gender, and professional sector influence participation, with youth and women demonstrating notably higher levels of engagement. Moreover, population density emerges as a critical factor in determining the appropriateness of event settings, with medium-sized gatherings in open spaces especially parks proving most effective. The findings emphasize the importance of designing inclusive and culturally responsive events, offering actionable insights for urban planning in rapidly growing cities. The study further highlights the need to reimagine neighborhood parks and open spaces as adaptable venues, equipped with essential infrastructure and governed by streamlined regulatory frameworks. Participants expressed a clear preference for accessible, medium-scale cultural events that prioritize safety, environmental sustainability, and enhanced public amenities, including transportation and sanitation services. Full article

While mechanical dewatering is widely used in faecal sludge treatment, the agricultural potential of mechanically dewatered faecal sludge (MDFS) combined with anaerobic digestion (AD) remains underexplored, particularly in sub-Saharan Africa where nutrient recovery is critical for food security. This study provides the first comprehensive characterisation of MDFS from Ghana’s largest treatment facility and evaluates anaerobic digestion effectiveness for agricultural application. Over six months, 182 composite MDFS samples from Lavender Hill Faecal Treatment Plant were analysed for physicochemical properties, nutrients, heavy metals, and microbial contaminants before and after AD treatment. MDFS demonstrated exceptional nutrient density, with total nitrogen (2141.05 mg/kg), phosphorus (190.08 mg/kg), and potassium (4434.88 mg/kg) concentrations comparable to commercial organic fertilisers. AD achieved significant pathogen reduction, decreasing total coliforms from 148,808.70 to 493.33 cfu/100 g (p < 0.001) and Ascaris lumbricoides eggs from 12.08 to 3.33 eggs/L, while maintaining nutrient integrity and keeping heavy metals within safe agricultural limits. Statistical modelling revealed a significant correlation between treatment duration and pathogen reduction efficiency. Despite substantial improvements, treated MDFS still exceeded some regulatory thresholds, indicating a need for complementary post-treatment strategies. This research establishes AD as an effective primary treatment for converting MDFS into a nutrient-rich organic fertiliser, supporting circular economy principles in urban sanitation systems and providing a sustainable pathway for agricultural nutrient recovery in resource-constrained settings. Full article

Chad, one of the poorest Sub-Saharan Central African countries, has one of the worst global diarrhea burdens. Project 21 seeks to enhance community health in the rural town of Béré, Chad but it is lacking. The study aims to determine diarrhea outcomes and associated factors, such as drinking water, malaria, sanitation and hygiene resources and practices, in Béré. A survey questionnaire was administered by trained community health workers using a random sampling method. The respondents (n = 484) are predominantly Nangtchéré (87%) evangelical (63%) males (88%) aged between 40–59 years old (43%) with secondary school education level (37%) or 8 years of school on average, from nuclear families (78%) with seven members on average, and of medium housing standard (56%). Drinking water treatment, transport and storage (p < 0.001), malaria related factors (p < 0.001), sanitation and hygiene practices (p < 0.001), children diarrhea experience, and treatment (p < 0.001) are predictors of diarrhea outcomes in adults. Also, factors related to drinking water transport, treatment and storage (p < 0.001), malaria (p < 0.001), health advice source (p < 0.001), sanitation and hygiene (p < 0.001), adult diarrhea experiences, and treatment (p < 0.001) are predictors of children diarrhea outcomes. Future interventions targeting the above factors are warranted. Full article

Poultry companies must implement measures to sanitize hatching eggs and reduce the risk of bacterial infections associated with poultry management. Many of them use formaldehyde (FA) fumigation in their egg sanitization protocols, but its toxicity has led to recommendations for reducing its use. However, studies employing this approach with liquid FA solutions in poultry operations, particularly during the hatching egg sanitization stage, remain scarce. Our objective was to evaluate whether sprayable FA reduces bacterial contamination on eggshells and whether it causes changes in their physical properties based on the analysis of microstructure, percentage relative to egg weight, and thickness. FA solutions at 0.5, 1, and 2% inhibited bacterial growth in vitro and reduced the bacterial load on the eggshell surface while also causing severe damage to the shell structure. Our results suggest that companies using FA should be aware of the associated risks, as significant production losses may be linked to the shell damage caused by this compound. Full article