Search Results (97)

Sea buckthorn is a valuable medicinal and edible plant, but the sour and astringent taste of its fruit limits the development of the related processing industry. This study focused on establishing the optimal composite enzymatic hydrolysis strategy to reduce acerbity during processing of sea buckthorn berries, while preserving the antioxidant activity. The results indicated that the most effective conditions for deacidification and deastringency were achieved with a pectinase-to-tannase mass ratio of 1:1, an enzyme dosage of 0.20%, at a pH of 4.50, a temperature of 50 °C, and a duration of 4 h. Under this treatment, the sea buckthorn could retain its potent antioxidant activity. Furthermore, a significant alteration was observed in the levels of 36 metabolites, which were correlated with the sensory attributes of the sea buckthorn. The findings of this study provided a theoretical basis for the enhanced utilization of sea buckthorn in processing and for a deeper understanding of its bioactive properties. Full article

To address the “3R” issues (resistance, resurgence, and residue) associated with chemical control of the sea buckthorn fruit fly (R. batava obscuriosa), this study proposes a novel physical barrier technology aimed at reducing pesticide application intensity, mitigating environmental pollution, and enhancing fruit quality. Yellow sticky traps were deployed to monitor adult occurrence dynamics and delineate the critical control window, while black polyethylene (PE) ground cover was installed on the orchard floor around the base of sea buckthorn trunks to prevent adult emergence from the soil. Control efficacy was evaluated by comparing adult trap catches and fruit infestation rates between the black PE ground cover treatment and the untreated control. Monitoring results revealed that adult emergence commenced on 29 June, entered the peak period on 9 July, attained maximum trap catch on 24 July, and persisted into the late emergence phase through mid-to-late August. Control data demonstrated that mean trap catches in the black PE ground cover treatment were lower than those in the control. From 2024 to 2025, fruit infestation rates declined from 74.5% and 62.3% in the control plot to 19.0~22.0% and 16.2~19.3% in the treatment plots, respectively, with control efficacy consistently exceeding 65%. This study demonstrates that black PE ground cover reduces adult abundance and fruit infestation rates of R. batava obscuriosa, with control efficacy consistently exceeding 65%. The observed effects are consistent with a soil-surface barrier effect and likely attributed to dual physical mechanisms: it may reduce adult emergence from the soil into the canopy and may obstruct mature larvae from entering the soil to pupate. This technology represents an environmentally sound, sustainable green control option suitable for integration into IPM programs for the sea buckthorn industry. Full article

Sea buckthorn (Hippophae rhamnoides L.) is a valuable plant whose fruits are rich in biologically active compounds. We sequenced the genome of variety Triumf of H. rhamnoides ssp. mongolica Rousi on the Oxford Nanopore Technologies (ONT) platform. With the Hifiasm algorithm optimized for ONT data, we assembled the 1.17-Gb genome into eleven complete chromosomes and one chromosome consisting of two contigs, which were scaffolded (Chr3). Eleven of twelve chromosomes had pronounced telomeric repeats at both ends and were assembled as telomere-to-telomere (T2T), and one chromosome (Chr12) had telomeric repeats only at one end. We also sequenced transcriptomes of thirteen Triumf organs/tissues and performed genome annotation using these and previously obtained RNA-Seq data for this variety. As a result, we predicted 25,915 genes and 30,527 transcripts. Repetitive elements comprised 66.9% of the genome size. The obtained near-T2T annotated genome assembly of H. rhamnoides ssp. mongolica variety Triumf enabled the identification of correct composition and sequences of important gene families in sea buckthorn. We demonstrated this with the FAT, SAD, and FAD gene families involved in fatty acid synthesis. Expression analysis revealed which FAT, SAD, and FAD genes are essential for specific organs/tissues. Thus, the Triumf genome assembly is a crucial tool for basic and applied studies of H. rhamnoides. Full article

This study examined the chemical composition and functional properties of fermented whey beverages produced from organic cow and goat whey, including both acid and sweet variants, enriched with organic sea buckthorn (Hippophae rhamnoides) or rosehip (Rosa canina) juices. In contrast to earlier research primarily addressing physicochemical and technological aspects, the present work offers a comprehensive evaluation of fatty acid composition, mineral and trace element content, antioxidant activity, and total polyphenol levels in these beverage formulations. Both the type of whey and the fruit additive significantly influenced the compositional profile and antioxidant capacity of the beverages. Samples fortified with rosehip demonstrated the highest antioxidant potential, as evidenced by enhanced DPPH radical scavenging activity, elevated FRAP values, and increased total polyphenol content. In comparison, beverages enriched with sea buckthorn juice exhibited higher concentrations of selected minerals, particularly Fe and Ni, while maintaining toxic element levels within permissible limits. The fatty acid profile was predominantly composed of saturated fatty acids, notably C16:0, along with short-chain fatty acids typical of whey. Beverages derived from goat whey contained higher levels of SFA and MUFA than those produced from cow whey, whereas the addition of rosehip contributed to increased proportions of PUFA and omega-3 fatty acids. Collectively, these findings indicate that organic fermented whey beverages enriched with rosehip or sea buckthorn juice may serve as promising functional products with enhanced antioxidant properties and favorable mineral and fatty acid profiles. Full article

Plant foods have been the cornerstone of human diets since ancient times, fueling civilization and shaping cultures. Plants became central to sustainable food systems, offering diverse and nutritious options for the future. Sea buckthorn (Hippophae rhamnoides L.) has attracted growing scientific interest due to the presence of bioactive compounds, polyphenols, fatty acids, phytosterols, carotenoids, vitamins, and minerals in its fruit, seeds, and leaves. Moreover, sea buckthorn exhibit antioxidant, anti-inflammatory, antimicrobial, antidiabetic, antihyperlipidemic, anticancer, hepatoprotective, neuroprotective, and metabolic regulatory properties supported by in vitro and in vivo models. The biological activity of these phytochemical compounds plays a crucial role in regulating the AMP-activated protein kinase (AMPK) and phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathways, as well as pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), cell proliferation, and apoptosis. Furthermore, its potential against microbial growth, including S. aureus, S. epidermidis, S. intermedius, and S. pyogenes, among others, not only expands its applications in the pharmaceutical industry but also attracts researchers to incorporate it into food products. This could lead to the discovery of plant-based therapeutic products without significant adverse effects. However, further exploration of each component’s potential side effects is necessary to support the commercialization of formulated products in either the pharmaceutical or food industries, ensuring the highest safety standards for consumers. Including studies on bioavailability and pharmacodynamics could further strengthen the scientific evidence supporting the specific phytochemicals in sea buckthorn and their mechanistic interactions. Full article

Aiming at addressing the lack of efficient, low-toxicity insecticides and precise application technologies in the current control of R. batava obscuriosa, this study intends to screen high-efficacy insecticides for UAV application and evaluate their field control effects, so as to provide a reference for the field control of R. batava obscuriosa. The residual film method was used to determine the laboratory toxicity of seven insecticides to adult R. batava obscuriosa. The LC₅₀ and LT₅₀ values of the seven insecticides were calculated. Based on these results, four high-efficacy insecticides were selected for UAV field efficacy testing. The control effects of different insecticides were evaluated through indicators such as population reduction rate, corrected control effect and fruit infestation rate after application. Laboratory toxicity results showed that 10% abamectin·beta-cypermethrin exhibited the strongest toxicity at 24 h (LC₅₀ = 22.108 mg/L). At 48 h after application, 5% emamectin benzoate was optimal (LC₅₀ = 5.486 mg/L). At 72 h after application, 10% broflanilide (LC₅₀ = 1.796 mg/L) and 10% abamectin·β-cypermethrin (LC₅₀ = 2.665 mg/L) performed best. Field test results indicated that the population reduction rate of 10% abamectin·β-cypermethrin reached 91.73% at 7 days after application, while the other three insecticides had population reduction rates above 80%, showing good overall control effect; at 15 days after application, 20% chlorfluazuron-thiamethoxam had the highest corrected efficacy (80.91%), and the fruit infestation rate of each insecticide group (24.75–27.49%) was significantly lower than that of the control group (58.08%). All four selected insecticides can effectively control the population of R. batava obscuriosa adults and can be used as quick- and long-acting insecticides against R. batava obscuriosa in production. Combined with UAV application technology, they can achieve efficient control of R. batava obscuriosa. Full article

Background: Sea buckthorn (Hippophae rhamnoides L.), the superfood of the present era, is widely recognized for its high content of nutrients and bioactive compounds. However, dietary products and by-products derived from different parts of the fruit differ markedly in their biochemical composition, which may influence their nutritional and biological effects. Drosophila melanogaster represents a well-established in vivo model for studying the impact of dietary components on nutritional status, development, and viability under defined nutritional conditions. Methods: Four sea buckthorn-derived matrices—seed flour, seed oil, pulp, and fruit pomace powder—were analyzed for fatty acid, amino acid, polyphenol, and antioxidant contents. Their effects were evaluated in D. melanogaster under zero-nutrient, normal-nutrient, and high-sugar diets, assessing viability and developmental dynamics across various product types and concentrations. Results: Substantial compositional differences were observed between the samples. Seed flour and fruit pomace powder were rich in proteins, essential amino acids, polyphenols, flavonoids, and condensed tannins, whereas seed oil predominantly contained fatty acids with limited antioxidant capacity. Consistent with these compositional profiles, diet- and product-specific biological effects were observed. Under zero-nutrient conditions, high concentrations of fruit pomace powder (100 g/L) supported larval and adult viability and resulted in developmental patterns comparable to those observed under a normal-nutrient diet. Under normal-nutrient and high-sugar diets, the matrices modulated development and viability without apparent toxicity, with fruit pomace powder consistently showing the most favorable effects. Conclusions: The biological responses of D. melanogaster are closely linked to the biochemical composition of the matrices and the dietary context. Fruit pomace powder emerged as the most effective product, highlighting its potential as a functional dietary ingredient and a valuable source of nutrients and bioactive compounds. Full article

Background/Objectives: Chronic idiopathic constipation (CIC) is a common gastrointestinal disorder with a global prevalence of about 14%, common in women and elderly population. It often lacks effective treatment. This randomized clinical trial was aimed to evaluate the efficacy and tolerability of Hippophae rhamnoides L. (sea buckthorn) fruit extract in adults with CIC. Methods: A UHPLC-HRMS/MS analysis was performed on the hydroethanolic H. rhamnoides fruit extract to evaluate its composition. Ninety participants were randomly assigned to receive either 500 mg of H. rhamnoides extract or placebo delivered through a capsule daily for 28 days. The primary outcome was the change in weekly spontaneous complete bowel movements (SCBMs), while secondary outcomes included stool consistency (Bristol Stool Form Scale—BSFS), gastrointestinal symptoms, and quality of life (SF-12). Results: Metabolic profile of the extract tentatively identified 75 bioactive compounds, predominantly flavonoids, triterpenoids and phospholipids. H. rhamnoides fruit extract significantly improved SCBM frequency (from 1.5 to 2.6 per week; p < 0.001) and normalized stool consistency (mean BSFS score from 1.4 to 3.5; p < 0.001), compared to no change in the placebo group. Significant reductions in bloating, abdominal pain, and heaviness were observed, while flatulence showed no between-group significant difference. No adverse events or use of rescue treatments were reported. Quality-of-life scores remained largely unchanged, with a non-significant trend towards improved mental health in the treated group. Conclusions: These findings suggest that H. rhamnoides fruit extract is a safe and effective option for managing CIC, offering an alternative to other plant extracts with laxative effects. Full article

Honey’s medicinal properties are largely attributed to its antioxidant activity, mainly derived from flavonoids, phenolic acids, and their derivatives. Fruit berries, such as goji berries, sea buckthorn, and black currant, are particularly rich in vitamins, phenolic compounds, and minerals, providing high nutritional and pharmacological value. Enrichment of rapeseed honey with dried fruits significantly increased total phenolic content, with the highest value observed in honey containing goji berries (111.221 ± 20.551 mg GAE/100 g), followed by black currant (96.477 ± 31.053 mg GAE/100 g) and sea buckthorn (90.724 ± 19.72 mg GAE/100 g), compared to control honey (49.681 ± 14.44 mg GAE/100 g). Antioxidant activity, assessed by multiple assays, was markedly enhanced in functional foods based on rape honey and dried fruits, particularly those with black currant, followed by goji berries and sea buckthorn. Romanian rapeseed honey contained phenolic acids such as gallic, chlorogenic, 4-hydroxybenzoic, and 3,4-dihydroxybenzoic acids, and flavonoids including quercetin and naringin. Functional food based on rape honey and goji berries (GBH) showed the highest levels of chlorogenic and gallic acids, epicatechin, and rutin, while functional food based on rape honey and sea buckthorn (SBH) was rich in naringin and resveratrol. Functional food based on rape honey and black currant (BCH) exhibited elevated gallic acid and rutin. Potassium and magnesium were the predominant minerals in all samples. Overall, berry enrichment enhances the nutritional and antioxidant profile of honey, supporting immune function and general health. Full article

Fruit shrubs’ lignocellulosic biomass remaining as waste after harvesting and/or after pruning is an underutilized, little-explored bioresource. Sea buckthorn (Hippophae rhamnoides L.), aronia (Aronia melanocarpa) and blackcurrant (Ribes nigrum) berries are rich in biologically active compounds, so these shrubs’ woody biomass derivatives are prospective investigation objects. The influence of pre-treated biomass, extracts, and purified proanthocyanidins on the oxidative stability of lipid-based systems was studied by accelerated oxidation method. Emulsion stability, antimicrobial activity against bacteria that causes acne—Cutibacterium acnes; contaminating wounds; skin care products—Streptococcus pyogenes, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus; cytotoxicity and phototoxicity of extracts and proanthocyanidins on HaCaT human keratinocytes were tested. The study established that biomass, lipophilic extracts obtained using liquefied hydrofluorocarbon, and hydrophilic extracts obtained by aqueous ethanol increased oxidative stability of lipid-based formulations. Compounds with skin-protecting properties were detected. Sea buckthorn and aronia hydrophilic extracts and proanthocyanidins had the highest antimicrobial activity. Low phototoxicity was revealed, emphasizing safety and applicability in topical formulations; human HaCaT keratinocyte viability was the best with aronia extracts, but none of the other samples decreased cell viability by more than 50%. It was proven that agro-waste biomass is a prospective source of multifunctional ingredients for cosmetic and pharmaceutical topical formulations. Full article

Sea buckthorn (Hippophae rhamnoides L.) fruit pomace flour (SBPF) was used as a partial replacement for light rye flour in rye bread production at levels of 5, 10, 15 and 20%. The aim of the study was to determine the effects of SBPF addition on sourdough fermentation dynamics, the development of the associated microflora (yeasts, moulds and lactic acid bacteria), and changes in selected quality attributes of the resulting breads. Fermentation was carried out for 72 h using a three-stage method, while monitoring pH, acidity and microbial counts. The results showed that SBPF addition lowered the initial pH of the sourdough and was associated with higher lactic acid bacteria (Lactobacillus spp.) counts (up to 8.96 log CFU/g at a 10% SBPF level), indicating prebiotic potential. Moderate SBPF addition, particularly at 10% (with an upper limit of 15%), ensured favourable bread quality characteristics, such as increased loaf volume, higher bread yield, improved water retention and a more intense crumb colour, whereas an SBPF level of 20% deteriorated loaf sphericity and weakened crumb structure. Multivariate PROFIT analysis indicated strong positive associations between SBPF level and crumb colour, bread yield and lactic acid bacteria counts, and negative associations with loaf sphericity and crumb compressibility. Therefore, the optimal range of SBPF substitution lies at approximately 10–15%. The obtained data support the use of sea buckthorn fruit pomace flour as a functional ingredient with a potentially beneficial effect on the technological and microbiological quality of rye bread. Full article

Farmers’ interest in producing sea buckthorn (Hippophae rhamnoides L.), a berry species with a high nutraceutical value, has increased in Poland and several other European countries, particularly under organic management. However, the expansion of the crop area has been paralleled by a higher occurrence particularly of the sea buckthorn fruit fly (Rhagoletis batava Hering, 1958), a tephritid species potentially causing severe damage to the berries. Trials to evaluate the mass trapping efficacy of various kinds of traps were carried out for up to five years in different locations, testing several attractants known to be effective with other fruit fly species. A commercial cone trap with an attractant designed for Ceratitis capitata and a homemade plastic bottle trap containing a 4% ammonium phosphate solution were found to be the most effective in attracting the flies in a preliminary trial and were further tested in various orchards for four years. Improved attractants were also assessed, together with the ammonium phosphate solution, but resulted in a limited increase in trapping. A reduction in the share of damaged fruits was observed in all trials, but with a generally low positive correlation with the number of catches, statistically significant only for the cone and bottle traps. The outcomes are discussed in view of the various factors determining mass trapping efficacy against flies of the family Tephritidae and the potential adoption of this control measure by farmers within an integrated pest management strategy, suitable to reduce the damage of the R. batava fly in organic sea buckthorn orchards, with an approach pursuing circular economy principles. Full article

The growing demand for ‘clean label’ functional foods necessitates the development of products that are not only health-promoting but also possess high sensory quality. Fruit mousses are an excellent matrix for fortification, appealing to a wide consumer base. This study aimed to enrich a conventional apple–plum mousse with vitamin D₃ and sea buckthorn oil, employing an encapsulation strategy based on endogenous fruit pectin. Three mousse variants were produced: a control (traditional), one fortified via the encapsulation of vitamin D₃ and sea buckthorn oil in a pectin-based nanoemulsion, and one fortified via the direct addition of the bioactive compounds. The products were analysed using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), colorimetry, texture analysis, rheology, fatty acid profiling, and sensory evaluation (profiling and ranking). SEM and FTIR analyses confirmed the successful formation of spherical submicron capsules (approx. 100–300 nm) within the fortified mousse. Encapsulation resulted in significantly lower colour change (ΔE = 6.07 ± 0.03) compared to direct addition (ΔE = 11.16 ± 0.03). The fortified mousses exhibited approximately 16–20% lower hardness (0.21–0.22 N vs. 0.25 N) and threefold lower adhesiveness (0.06–0.08 N·s vs. 0.19 N·s) compared to the control. Rheological analysis indicated that fortification did not compromise the structural stability of the mousses (G′ > G″ across 0.1–10 Hz). The fatty acid profile was significantly improved in the fortified variants, with a three-fold increase in linoleic acid (C18:2 n-6 c: from 16.90% to 55–56%) and the introduction of γ-linolenic acid (C18:3 n-6: 0.38–0.39%). Sensory ranking revealed no significant differences in overall quality between the control and fortified mousses (p > 0.05). Pectin present in fruits can be effectively utilised to encapsulate vitamin D₃ and sea buckthorn oil, allowing the successful development of a functional fruit mousse with an improved nutritional profile and retained sensory quality. Encapsulation proved to be a superior fortification method, offering better protection of bioactive compounds and a lesser impact on the product’s original colour. Full article

To address longstanding challenges in sea buckthorn harvesting—such as the absence of effective harvesting principles, inefficient traditional manual and semi-mechanised methods, and rising labour costs—this study developed a self-propelled harvester equipped with a reciprocating cutter. The harvester featured an optimised double-support reciprocating cutter driven by a swing ring mechanism, with its kinematic parameters and cutting speed determined through analytical analysis. A coordinated transport system, consisting of an arc-shaped branch dial wheel, a conveying device, and a hydraulic system, was also designed. Field experiments were conducted employing a three-factor, three-level Box–Behnken design of Response Surface Methodology (RSM), which enabled the establishment of a predictive mathematical model for harvesting performance. Numerical optimisation via the model yielded the optimal operational parameters: harvesting forward speed of 0.6 m·s⁻¹, a cutting speed of 1.2 m·s⁻¹, and a conveyor belt linear speed of 0.8 m·s⁻¹. With this parameter combination, the missed cutting rate was 6.72%, fruit breakage rate 4.06%, and conveyor failure rate 7.79%, all meeting mechanised harvesting standards. This research provides the essential theoretical foundation and technical solutions for harvesting equipment in the sea buckthorn industry, accelerating its mechanisation process. Full article

This study employed wild sea buckthorn (Hippophae rhamnoides L.) fruits harvested in Qinghai Province as experimental material. Following compositional analysis of their flavonoids, the antibacterial efficacy and mechanistic pathways of flavonoids in sea buckthorn against Helicobacter pylori (H. pylori) were systematically examined through in vitro and animal model experiments. The results showed that the main flavonoids in sea buckthorn were rutin, quercetin-3-O-glucoside, quercetin, catechin, isorhamnetin, kaempferol-3-O-glucoside, kaempferol and epicatechin. Among them, quercetin, isorhamnetin and kaempferol had strong inhibitory activity against H. pylori. In vitro, sea buckthorn flavonoids inhibited the growth of H. pylori through multiple mechanisms: inducing morphological transformation from rod-shaped to spherical bacteria, promoting cell shrinkage and rupture, disrupting the cell membrane to cause leakage of intracellular macromolecules, increasing membrane permeability, and suppressing urease activity. Sea buckthorn flavonoids exert therapeutic effects on H. pylori-infected mice through multiple mechanisms, including the alleviation of gastric mucosal inflammation via the Nuclear Factor KappaB (NF-κB) signaling pathway, the down-regulation of gastrin-17 (GAS17) to suppress gastric acid production, and the up-regulation of epidermal growth factor (EGF) expression to promote gastric mucosal repair and modulate the composition of gastric microbiota. This study systematically elucidated the anti-H. pylori activity and antibacterial mechanisms of flavonoids derived from sea buckthorn fruits, providing a theoretical basis for the screening of natural antibacterial agents from this plant source. Full article