Search Results (298)

Musa haekkinenii is a compact wild banana species with emerging value in ornamental horticulture, yet its adaptive responses to environmental factors remain underexplored. This study investigated the morpho-physiological and anatomical responses of M. haekkinenii to contrasting light regimes and irrigation water qualities to identify optimal cultivation conditions. A 210-day factorial experiment was conducted under subtropical greenhouse conditions using a split-plot design, with light intensity (full sun vs. shade) and irrigation water quality (reverse osmosis vs. well water) as treatment factors. Plants grown under shaded conditions and irrigated with reverse osmosis water exhibited significant increases in plant height, pseudostem diameter, leaf number, and sucker production, alongside enhanced pigment accumulation and photosynthetic performance. In contrast, full-sun plants irrigated with well water showed reduced growth, lower photosynthetic efficiency, and increased substrate salinity, indicating additive effects of light and osmotic stress. Leaf anatomical analysis revealed greater stomatal size and density under shade, particularly when combined with high-quality irrigation. Multivariate analysis further supported the association of favorable trait expression with shaded conditions and reverse osmosis water. These findings highlight the importance of microenvironmental management in enhancing the physiological stability and ornamental quality of M. haekkinenii, supporting its potential application in sustainable urban landscaping. Full article

TCP transcription factors represent a crucial family of plant regulators that contribute significantly to growth and developmental processes. Although the TCP gene family has been extensively studied in various plant species, research on Chimonanthus praecox (wintersweet) remains limited. Here, we performed genome-wide identification and analysis of the TCP gene family in C. praecox and identified 22 CpTCP genes. We further systematically examined the associated physicochemical properties, evolutionary relationships, gene structures, and regulatory features. Analysis revealed that all CpTCP proteins possess a conserved TCP domain, and subcellular localization prediction indicated their localization in the nucleus. Promoter analysis revealed that multiple cis-elements are associated with abiotic stress responses and plant growth regulation. Further analysis revealed high CpTCP2 expression in the leaves and stamen, with significantly increased levels during flower senescence. CpTCP2 expression was upregulated in response to methyl jasmonate (MeJA), salicylic acid, abscisic acid, and shade. CpTCP2 overexpression in Arabidopsis thaliana resulted in a reduced leaf area, delayed flowering, and increased rosette leaf numbers. Moreover, MeJA treatment accelerated leaf senescence in CpTCP2 transgenic Arabidopsis. These findings provide insights into the evolutionary characteristics of the TCP family in C. praecox, highlighting the functional role of CpTCP2 in regulating leaf development and flowering time in Arabidopsis, thereby offering valuable genetic resources for wintersweet molecular breeding. Full article

The building sector plays a key role in the transition toward climate neutrality, with national regulations across the EU requiring the construction of nearly zero-energy buildings (nZEBs). However, while energy performance has been extensively studied, less attention has been given to the problem of ensuring user comfort—both indoors and in the surrounding outdoor areas—under nZEB design constraints. This gap raises two key research objectives: (1) to evaluate whether a well-designed nZEB with extensive glazing maintains acceptable indoor thermal comfort and (2) to assess whether residents experience greater outdoor thermal comfort and satisfaction in small, sun-exposed private gardens or in larger, shaded communal green spaces. To address these objectives, a newly built residential estate near Kraków (Poland) was analyzed. The investigation included simulation-based assessments during the design phase and in situ measurements during building operation, complemented by a user survey on spatial preferences. Indoor comfort was evaluated for rooms with large glazed façades, as well as rooms with standard-sized windows, while outdoor comfort was assessed in both private gardens and a shared green courtyard. Results show that shading the southwest-oriented glazed façade with an overhanging terrace provided slightly lower temperatures in ground-floor rooms compared to rooms with standard unshaded windows. Outdoors, users experienced lower thermal comfort in small, unshaded gardens than in the larger, vegetated communal area (pocket park), which demonstrated greater capacity for temperature moderation and thermal stress reduction. Survey responses further indicate that potential future residents prefer the inclusion of a shared green–blue infrastructure area, even at the expense of building some housing units in semi-detached form, instead of maximizing the number of detached units with unshaded individual gardens. These findings emphasize the importance of addressing both indoor and outdoor comfort in residential nZEB design, showing that technological efficiency must be complemented by user-centered design strategies. This integrated approach can improve the well-being of residents while supporting climate change adaptation in the built environment. Full article

The rapid urbanization in developing countries has resulted in altered land-use patterns, surface energy imbalances, and heightened urban heat stress, exacerbating the urban heat island effect and vulnerability to heatwaves. The abandonment of agricultural lands, while a global challenge, presents cities with a unique opportunity to meet tree cover targets and improve resilience to these climatic challenges. Building on prior studies, this research employs the combined use of ENVI-met 4.4.6 and Ray-Man 3.1 simulation models to assess the efficacy of nature-based solutions in revegetating abandoned urban agricultural lands with the aim of enhancing outdoor thermal comfort. As a vital component of urban ecosystem services, thermal comfort, particularly through microclimate cooling, is essential for improving public health and livability in cities. This investigation focuses on the integration of broadleaf, evergreen, and edible woody species as bioclimatic interventions to mitigate urban heat stress. Simulation results showed that species such as Quercus spp. (broadleaf) and Cupressus arizonica (evergreen) substantially reduced the Mean Radiant Temperature (Tmrt) index by up to 26.76 °C, primarily due to their shading effects and large canopies. Combining these vegetation types with crops emerged as the most effective strategy to mitigate heat stress and optimize land-use. This study demonstrates how cities can incorporate nature-based solutions to adapt and mitigate the health risks posed by climate change while fostering resilience. These findings offer valuable knowledge for other developing countries facing similar challenges, highlighting the importance of revegetating abandoned urban agricultural lands for thermal comfort and ecosystem service provision, with the advantages of reducing mortality and morbidity during heatwaves. Consequently, these results should inform urban climate policies aimed at promoting resilience, public health, and ecological sustainability in a changing climate. Full article

Shaded resting zones in rotational grazing systems are prone to thermal stress due to limited ventilation and the congregation of animals during peak heat periods. Addressing these challenges requires sensing solutions that are not only accurate but also capable of adapting to dynamic environmental conditions and energy constraints. In this context, we present the development and simulation-based validation of a self-configurable IoT protocol for adaptive environmental monitoring. The approach integrates embedded machine learning, specifically a Random Forest classifier, to detect critical conditions using synthetic data of temperature, humidity, and CO₂. The model achieved an accuracy of 98%, with a precision of 98%, recall of 85%, and F1-score of 91% in identifying critical states. These results demonstrate the feasibility of embedding adaptive intelligence into IoT-based monitoring solutions. The protocol is conceived as a foundation for integration into physical devices and subsequent evaluation in farm environments such as rotational grazing systems. Full article

Soybean (Glycine max (L.) Merr.) is a vital crop for the global supply of protein and oil, with its growth and development being regulated by genetic, hormonal, and environmental factors, particularly light and hormone signaling. The Small Auxin-Up RNA (SAUR) gene family plays a crucial role in plant growth regulation; however, the molecular mechanisms by which GmSAUR46 integrates photosynthesis and hormonal networks in soybean remain unclear. In this study, we focused on GmSAUR46b (Glyma.19G182600.1) and employed CRISPR/Cas9-mediated knockout and 35S-driven overexpression lines, alongside wild-type soybean (cv. Williams 82), to investigate its function. RNA sequencing (RNA-Seq) was conducted on shoot apical meristems, stems, and leaves at three developmental stages (V1, V2, V3), followed by transcriptomic analyses, including differential gene expression (DEG) identification and functional enrichment (GO, KEGG, KOG). Anatomical studies using paraffin sectioning and scanning electron microscopy (SEM) assessed the leaf midrib thickness and stem trichome density under varying light conditions. The transcriptomic results revealed DEGs enriched in pathways related to cell wall metabolism, hormone response, and photosynthesis. Anatomical analyses demonstrated that GmSAUR46b specifically regulates the leaf midrib thickness and stem trichome density in a light-dependent manner: under shade, the overexpression lines exhibited increased midrib thickness and trichome density, whereas the knockout lines showed reduced trichome density. Additionally, novel transcripts associated with stress resistance, hormone metabolism, and photosynthesis were identified, expanding the known soybean gene repertoire. Collectively, GmSAUR46b functions as a central hub integrating light signals with hormone and cell wall pathways to modulate soybean growth, particularly leaf and stem traits. This study advances understanding of SAUR gene function in soybean and provides valuable insights for molecular breeding aimed at improving adaptability and yield under diverse environmental conditions. Full article

Ulmus pumila L. ‘Zhonghua Jinye’ is a plant variety with colourful leaves that is widely used in landscaping. In our study, the leaves of U. pumila ‘Zhonghua Jinye’ fade and turn green under light (LT, 45%), moderate (MD, 70%) and high (HG, 95%) shading treatment, reducing its ornamental value. However, the mechanism underlying this adaptation to shade is poorly understood. The objective of this study was to elucidate the mechanism of physiological, cellular microstructural and transcriptional changes involved in leaf regreening in U. pumila ‘Zhonghua Jinye’. Our results showed that the pigment content of U. pumila ‘Zhonghua Jinye’ leaves increased under shade stress, with a corresponding colour change from yellow to dark green. Thus, U. pumila ‘Zhonghua Jinye’ adapted to shade stress by increasing leaf pigment and chlorophyll content. Transmission electron microscopy showed that thylakoid stacking in the grana lamellae changed significantly from a loose state to a closely packed structure under shaded conditions. Because plant pigments were located mainly in thylakoids, this closer stacking increased photosynthetic efficiency and pigment accumulation. RNA sequencing analysis showed that Lhcb1, a key thylakoid membrane gene, was upregulated under shade, which promoted thylakoid stacking and light absorption. In the chlorophyll synthesis pathway, haeme metabolism was inhibited, increasing protoporphyrin IX flow to the chlorophyll pathway and promoting the synthesis of chlorophyll a/b. The simultaneous upregulation of plant hormone-related genes promoted an increase in plant leaf area, improving the light energy utilisation ratio. This study is the first to report the self-regulatory mechanism that leads to colour change in U. pumila ‘Zhonghua Jinye’ under shade stress and provides a theoretical basis for the cultivation of tree species with colourful leaves. Full article

Spring droughts, increasingly coinciding with canopy shade, interactively stress the growth of urban tree species and are poorly understood in Beijing. Three-year-old saplings of Pinus tabuliformis and Robinia pseudoacacia were subjected to comparative analysis under four drought–shade sequences, with a full-light, well-watered treatment serving as the control. During two periods encompassing the drought to wilting point and subsequent rewatering, we assessed leaf morphology, water status, photosynthetic gas exchange, and chlorophyll fluorescence. Both species exhibited losses in leaf water and carbon assimilation under drought, yet their adaptive strategies substantially differed. P. tabuliformis conserved water through the stable leaf anatomy and conservative stomatal control. In particular, P. tabuliformis under full-light and drought conditions decreased their specific leaf area (SLA) by 23%, as well as showing reductions in stomatal conductance (G_s) and transpiration rate (T_r) along with the drought duration (p < 0.01). As the duration of post-drought rewatering increased, the reductions in the net photosynthetic rates (P_n) of P. tabulaeformis showed that the shade condition intensified its photosynthetic limitation and slowed recovery after drought. Under low-light drought, R. pseudoacacia exhibited a 52% increase in SLA and a 77% decline in G_s; the latter was markedly smaller than the reduction observed under full-light drought. After rewatering, G_s displayed an overcompensation response. The rise in specific leaf area and the greater flexibility of stomatal regulation partly offset the adverse effects of drought. Nevertheless, post-drought P_n recovered to only 40%, significantly lower than the 61% recovery under full-light drought. Moreover, the negative correlation between SLA and P_n became significantly stronger, indicating that the “after-effects” of shade–drought hindered photosynthetic recovery once the stress was relieved. Drought duration eroded the phenotypic performance in both species, while the light environment during drought and subsequent rehydration determined the time trajectory and completeness of recovery. These results validate a trade-off between shade mitigation and drought legacy, and guide species selection: plant shade-tolerant R. pseudoacacia in light-limited urban pockets and reserve sun-dependent P. tabuliformis for open, high-light sites to enhance drought resilience of Beijing’s urban forests. Full article

The Western Reef Heron (Egretta gularis) has a wide geographic distribution, ranging from the coasts of West Africa to Southwest Asia, including the Arabian Peninsula. Despite this extensive range, detailed information on its incubation behavior remains scarce. To address this gap, we investigated the 24 h incubation behavior of Western Reef Herons on Al-Fanateer Island, Eastern Saudi Arabia, during early summer—a period characterized by pronounced diurnal fluctuations in ambient temperature. Using trail cameras and temperature loggers, we found that adults maintained nearly continuous attendance at the nest throughout the day, with incubation coverage exceeding 97% across all two-hour intervals. A slight reduction in nest attendance was observed during nighttime (lowest at 86.8% between 20:00–21:59). Incubating adults exhibited behavioral plasticity in response to ambient temperature: a sitting posture was predominant during cooler periods, while a shading posture was more frequent during peak heat. Incubating adults also adjusted their orientation with the solar angle, actively avoiding southern and western exposures during the hottest parts of the day. Despite substantial variation in ambient temperature, the temperature beneath the clutch ranged from 29.4 to 37.8 °C, which may indicate effective thermoregulation. These findings suggest that a combination of near-continuous nest attendance, posture adjustment, and solar orientation avoidance allows Western Reef Herons to mitigate thermal stress and maintain optimal conditions for embryo and chick development. We recommend long-term monitoring of incubation behavior in this species to further evaluate its adaptability to environmental changes, particularly those driven by climate variability. Full article

Singlet oxygen (¹O₂), a reactive oxygen species, can oxidize lipids, proteins, and DNA at high concentrations, leading to cell death. Despite its extremely short half-life (10⁻⁵ s), ¹O₂ acts as a critical signaling molecule, triggering a retrograde pathway from chloroplasts to the nucleus to regulate nuclear gene expression. In this study, rice seeds were treated with 0, 5, 20 and 80 μM Rose Bengal (RB, a photosensitizer) under moderate light for 3 days to induce ¹O₂ generation. Treatment with 20 μM RB reduced stomatal density by approximately 25% in three-leaf-stage rice seedlings, while increasing the contents of pectin, hemicellulose, and cellulose in root cell walls by 30–40%. Under drought, salinity, or shading stress, 20 μM RB treatment significantly improved rice tolerance, as evidenced by higher relative water contents (49–58%) and chlorophyll contents (60–76%) and lower malondialdehyde (37–43%) and electrolyte leakage (29–37%) compared to the control. Moreover, RT-qPCR analysis revealed that the significant up-regulation of stomatal development genes (OsTMM and OsβCA1) and cell wall biosynthesis genes (OsF8H and OsLRX2) was associated with RB-induced ¹O₂ production. Thus, under controlled environmental conditions, ¹O₂ may regulate stomatal development and cell wall remodeling to enhance rice tolerance to multiple abiotic stresses. These results provide new perspectives for the improvement of rice stress tolerance. Full article

To elucidate shade adaptation mechanisms in Pinus yunnanensis seedlings across different ages, this study established five light gradients (100% full sunlight as control or CK, 80% as L1, 45% as L2, 30% as L3, and 5% as L4) for experimental treatments on one- and three-year-old seedlings. By analyzing dynamic changes in non-structural carbohydrates (NSCs) and their components within needles, stems, and roots—combined with a phenotypic plasticity assessment, a correlation analysis, and a principal component analysis—we explored the carbon metabolic adaptations under shade stress. The key results demonstrate the following: (1) Increasing shade intensity significantly reduced the NSCs in the needles and roots of both age groups. The stem NSCs markedly decreased under L1 and L2, indicating “carbon limitation.” However, under severe shade (L3 and L4), the stem NSCs stabilized while the stem soluble sugars gradually increased. In three-year-old Pinus yunnanensis seedlings under the L3 treatment, the ratio of soluble sugars to starch in the stems reached as high as 5.772 g·kg⁻¹, yet the stem NSC content showed no significant change. This pattern exhibited “growth stagnation-carbon enrichment” characteristics. This reveals a physiological strategy for maintaining stem carbon homeostasis through a “structure–metabolism” trade-off under carbon limitation. (2) Shade adaptations diverged by age: one-year-old seedlings employed a short-term “needle–root source–sink reallocation” strategy, whereas three-year-old seedlings developed a “root–stem–needle closed-loop homeostasis regulation” mechanism. (3) Age-specific shade thresholds were identified: one-year-old seedlings required >80% full light to maintain a carbon balance, while three-year-old seedlings exhibited enhanced root carbon storage under moderate shade (45–80% full light). This study clarifies the physiological mechanisms by which P. yunnanensis seedlings of varying ages optimize shade adaptation through organ-specific carbon allocation, providing a theoretical foundation for shade management in artificial forests and understory seedling conservation. Full article

This paper presents a literature review of low-power hybrid wind–photovoltaic (PV) systems and introduces a 3 m diameter prototype rotor featuring twelve PV-coated pivoting blades stiffened by a peripheral rim. Existing solutions—foldable umbrella concepts, Darrieus rotors with PV-integrated blades, and morphing blades—are surveyed, and current gaps in simultaneous wind + PV co-generation on a single moving structure are highlighted. Key performance indicators such as power coefficient (C_p), DC ripple, cell temperature difference (ΔT), and levelised cost of energy (LCOE) are defined, and an integrated assessment methodology is proposed based on blade element momentum (BEM) and computational fluid dynamics (CFD) modelling, dynamic current–voltage (I–V) testing, and failure modes and effects analysis (FMEA) to evaluate system performance and reliability. Preliminary results point to moderate aerodynamic penalties (ΔC_p ≈ 5–8%), PV output during rotation equal to 15–25% of the nominal PV power (P_PV), and an estimated 70–75% reduction in blade–root bending moment when the peripheral ring converts each blade from a cantilever to a simply supported member, resulting in increased blade stiffness. Major challenges include the collective pitch mechanism, dynamic shading, and wear of rotating components (slip rings); however, the suggested technical measures—maximum power point tracking (MPPT), string segmentation, and redundant braking—keep performance within acceptable limits. This study concludes that the concept shows promise for distributed microgeneration, provided extensive experimental validation and IEC 61400-2-compliant standardisation are pursued. This paper has a dual scope: (i) a concise literature review relevant to low-Re flat-blade aerodynamics and ring-stiffened rotor structures and (ii) a multi-fidelity aero-structural study that culminates in a 3 m prototype proposal. We present the first evaluation of a hybrid wind–PV rotor employing untwisted flat-plate blades stiffened by a peripheral ring. Using low-Re BEM for preliminary loading, steady-state RANS-CFD (k-ω SST) for validation, and elastic FEM for sizing, we assemble a coherent load/performance dataset. After upsizing the hub pins (Ø 30 mm), ring (50 × 50 mm), and spokes (Ø 40 mm), von Mises stresses remain < 25% of the 6061-T6 yield limit and tip deflection ≤ 0.5%·R acrosscut-in (3 m s⁻¹), nominal (5 m s⁻¹), and extreme (25 m s⁻¹) cases. CFD confirms a broad efficiency plateau at λ = 2.4–2.8 for β ≈ 10° and near-zero shaft torque at β = 90°, supporting a three-step pitch schedule (20° start-up → 10° nominal → 90° storm). Cross-model deviations for C_p, torque, and pressure/force distributions remain within ± 10%. This study addresses only the rotor; off-the-shelf generator, brake, screw-pitch, and azimuth/tilt drives are intended for later integration. The results provide a low-cost manufacturable architecture and a validated baseline for full-scale testing and future transient CFD/FEM iterations. Full article

Heat stress is one of the main welfare and productivity problems faced by dairy cattle in Mediterranean climates. The main objective of this work is to predict heat stress in livestock from shade-seeking behavior captured by computer vision, combined with some climatic features, in a completely non-invasive way. To this end, we evaluate two soft computing algorithms—Random Forests and Neural Networks—clarifying the trade-off between accuracy and interpretability for real-world farm deployment. Data were gathered at a commercial dairy farm in Titaguas (Valencia, Spain) using overhead cameras that counted cows in the shade every 5–10 min during summer 2023. Each record contains the shaded-cow count, ambient temperature, relative humidity, and an exact timestamp. From here, three thermal indices were derived: the current THI, the previous-night mean THI, and the day-time accumulated THI. The resulting dataset covers 75 days and 6907 day-time observations. To evaluate the models’ performance a 5-fold cross-validation is also used. The results show that both soft computing models outperform a single Decision Tree baseline. The best Neural Network (3 hidden layers, 16 neurons each, learning rate $={10}^{-3}$) reaches an average RMSE of $14.78$, while a Random Forest (10 trees, depth $=5$) achieves $14.97$ and offers the best interpretability. Daily error distributions reveal a median RMSE of $13.84$ and confirm that predictions deviate less than one hour from observed shade-seeking peaks. Although the dataset came from a single farm, the results generalized well within the observed range. However, the models could not accurately predict the exact number of cows in the shade. This suggests the influence of other variables not included in the analysis (such as solar radiation or wind data), which opens the door for future research. Full article

Colored shade nets have gained attention due to their ability to reduce light intensity and alter the light spectrum, thereby influencing vegetable crop quality and yield. However, limited research has examined their effects on jalapeño pepper (Capsicum annuum L.) growth and yield. This study evaluated the impact of four nets—black, red, silver, and white (40% shade factor)—compared to an unshaded control. The red net altered light quality by increasing the proportion of red and far-red wavelengths, while the other nets reduced light intensity without spectral modification. Although differences in mean air temperature were minimal between shaded and unshaded conditions, root zone temperatures were consistently lower under shade nets. Shade treatments significantly increased plant height, stem diameter, and leaf chlorophyll content relative to the unshaded control. The highest rates of leaf transpiration and stomatal conductance were recorded under unshaded and white net conditions. Net photosynthesis, electron transport rate, intercellular CO₂ concentration, or photosynthetic water use efficiency were similar among net treatments. Marketable and total yields did not differ significantly among net treatments in either year; however, in 2021, they were positively associated with light intensity. In conclusion, while colored shade nets promoted vegetative growth, they did not enhance fruit yield relative to unshaded conditions in jalapeño pepper. Full article

Picea neoveitchii Mast., a critically endangered spruce species endemic to China, is classified as a national second-level key protected wild plant and listed as critically endangered (CR) on the International Union for Conservation of Nature (IUCN) Red List. Its habitat features complex forest light environments, and global climate change coupled with environmental pollution has increased regional nitrogen deposition, posing significant challenges to its survival. This study explores the effects of light–nitrogen interactions on the leaf functional traits of Picea neoveitchii Mast. seedlings by simulating combinations of light intensities (100%, 70%, and 40% full sunlight) and nitrogen application levels (0, 10, and 20 g N·m ⁻²·a⁻¹, where g N·m⁻²·a⁻¹ denotes grams of nitrogen applied per square meter per year). We examined changes in morphological traits, anatomical structures, photosynthetic physiology, and stress resistance traits. Results indicate that moderate shading (70% full sunlight) significantly enhances leaf morphological traits (e.g., leaf length, leaf area, and specific leaf area) and anatomical features (e.g., mesophyll tissue area and resin duct cavity area), improving light capture and stress resistance. Medium- to high-nitrogen treatments (10 or 20 g N·m⁻²·a⁻¹) under moderate shading further increase photosynthetic efficiency, stomatal conductance, and antioxidant enzyme activity. According to the comprehensive membership function evaluation, the L2N0 (70% full sunlight, 0 g N·m⁻²·a⁻¹) treatment exhibits the most balanced performance across both growth and stress-related traits. These findings underscore the critical role of light–nitrogen interactions in the growth and adaptability of Picea neoveitchii Mast. leaves, offering a scientific foundation for the conservation and ecological restoration of endangered plant populations. Full article