Search Results (7,517)

The Inner Mongolian Plateau is a critical region for the development of herbivorous animal husbandry in China. However, its harsh climate and poor soil quality have constrained the sustainable growth of the alfalfa industry. This 3-year field study investigated the effects of potassium (K) fertilizer on the productivity and forage quality of alfalfa (Medicago sativa L. cv. ‘WL168’) in such specific conditions of the region. Five rates of K fertilizer (0 (CK), 100, 200, 300, and 400 kg ha⁻¹ of K₂O) were applied in three split applications. Forage harvests occurred three times annually in 2023 and 2024, and yield, yield components, and forage quality were determined. The results showed that the forage yield of alfalfa increased initially and then decreased with the rising K application rates, which paralleled the changes in the plant density, and plant height, especially the mass shoot⁻¹; forage yield was mainly correlated with mass shoot⁻¹. Appropriate K fertilizer improved forage quality, especially in 2024. With increasing application, crude protein (CP) and total digestible nutrients (TDNs) first rose then declined, whereas neutral detergent fiber (NDF) and acid detergent fiber (ADF) decreased steadily, leading to a consistent rise in the relative feeding value (RFV). Comprehensively considering both yield and quality under such condition, a K fertilizer application rate of 273.2 kg ha⁻¹ of K₂O is suggested as a reference for this region. Full article

Tanacetum balsamita L. is a medicinal and aromatic plant of high economic value, yet its tissue culture and micropropagation protocols remain poorly developed. This study evaluated and compared two in vitro culture systems, semisolid medium (SS) and Temporary Immersion System (TIS), for enhancing biomass production and growth performance, in terms of relative growth rate (RGR), photosynthetic activity, chlorophyll content, antiradical capacity, and anatomical development. The results demonstrated that the TIS significantly improved RGR, photosynthetic performance, and antiradical activity, and promoted the anatomical development that facilitated greenhouse acclimatization. Machine learning (ML) models, including Multilayer Perceptron (MLP) and Random Forest (RF), were employed to predict morphological and biochemical traits. MLP achieved the highest predictive accuracy (R² > 0.95) and lowest error metrics for complex, nonlinear traits such as chlorophyll content and antiradical activity, whereas RF excelled in predicting morphological traits with more uniform variance, such as leaf number and shoot length. Overall, this study demonstrates that the TIS provides a high-yield, economically crucial strategy for the micropropagation of T. balsamita, and that integrating ML-based predictive modeling can enhance parameter optimization and phenotyping precision. This combined approach offers a valuable framework for advancing tissue culture research in medicinal and aromatic plants through both production efficiency and data-driven decision-making. Full article

Cadmium (Cd) contamination of soil threatens agricultural productivity and food safety. In this study, a dual-component remediation strategy combining lanthanum-cysteine chelate (CLa) and corn steep liquor (CSL) was developed to alleviate Cd toxicity in Chinese cabbage (Brassica rapa subsp. pekinensis). CLa enhanced photosynthetic efficiency, antioxidant enzyme activity, and root viability, while reducing Cd translocation to shoots. In contrast, CSL acted primarily through organic nutrient supplementation, stimulating chlorophyll synthesis and promoting the growth of beneficial rhizosphere microbes. Notably, the combined treatment (CLCS) exhibited a synergistic effect, significantly enhancing biomass production, nutrient uptake, photosynthetic performance, and oxidative stress tolerance, while reducing Cd accumulation in plant tissues. Furthermore, CLCS optimized the soil microenvironment and microbiota composition, reinforcing plant resilience under Cd stress. This study offers a promising and cost-effective approach for mitigation of heavy metal stress and crop productivity improvement by coordinated plant–microbe–soil interactions. Full article

Napier grass (Cenchrus purpureus Schumach) is an important forage crop and livestock feed. However, its yield and quality in Kenya are often limited by Napier grass headsmut and stunt disease. Napier grass genetic improvements through mutation breeding and selection could avail cultivars with increased forage. This study investigated the response of embryogenic calli to different levels of colchicine in inducing polyploidy in the two germplasms of Napier grass; South africa and Bana grass. The experiments were carried out as a factorial experiment in a completely randomized design (CRD). The colchicine concentrations used were 0, 0.05, 0.1, and 0.2%, and the exposure durations were 24, 48, and 72 h. During the shoot regeneration stage, culturing explants on an MS medium (Murashige and Skoog) supplemented with 0.2 mg L⁻¹ Benzyl Adenine (BAP), 0.1 mg L⁻¹ dichlorophenoxyacetic acid (2, 4-D), and 0.1 mg L⁻¹ indole-3-butyric acid (IBA) was more suitable for shoot regeneration. Chromosome doubling was confirmed by genomic DNA and the stomata size and number. Culturing explants on an MS medium supplemented with 1 mg L⁻¹ IBA, 1 mg L⁻¹ 2, 4-D, and 0.5 mg L⁻¹ BAP was more suitable in inducing embryogenic calli in both genotypes. Polyploidy results revealed that a 0.1% concentration of colchicine with two days of treatment established the maximum number of octoploid plantlets induced in vitro, while a 0.2% concentration was very toxic. The stomata size and number of derived octoploid plantlets were bigger with a lower density, a shorter plant height, and a smaller stem diameter, and despite being the first to produce tillers, they were significantly higher than their progenitors. Induced mutants also had a significantly higher number of chromosomes and showed different band patterns and distances during gel electrophoresis. However, we recommend the use of flow cytometry to confirm the ploidy level. The superior mutant plantlets can be selected and recommended for characterization across representative agro-ecologies for large-scale production and used in Cenchrus purpureus breeding programs in Kenya and its environments. Full article

This study explores the complex regulatory mechanisms of nitrogen (N) and phosphorus (P) supply interactions on the growth, root architecture, and nutrient uptake of Populus × euramericana ‘Neva’ seedlings. It shows that these responses depend on nutrient concentrations and exhibit organ-specific patterns. Low P (0 mM) and sufficient N (15–30 mM) enhances plant height and aboveground biomass by promoting P acquisition processes. At moderate N levels (5–15 mM), P supply is sufficient (0.5–1.5 mM) for root and stem growth. Nitrogen application prioritizes aboveground biomass, reducing the root-to-shoot ratio. Root architecture also responds organ-specifically: sufficient N under low P promotes fine root growth to increase P absorption; under moderate P (0.5 mM), balanced N optimizes branching; and under sufficient P (1.5 mM), N increases root thickness while reducing fine root investment. In terms of P metabolism, moderate N under low P increases P concentrations by upregulating phosphate transporter genes, while sufficient N maintains P use efficiency (PUE). For N metabolism, added P under low N (0 mM) maintains N use efficiency (NUE), while higher N levels (15–30 mM) reduce NUE due to interference in nitrogen transport and enzyme activity. This study highlights the importance of organ-specific resource allocation in adapting to N–P interactions and suggests optimizing fertilization strategies based on soil nutrient status to avoid physiological imbalance. Full article

Drought stress is one of the primary abiotic factors negatively affecting garlic growth, development, and yield formation. The application of plant growth-promoting bacteria (PGPB) could enhance plant tolerance to drought stress. The aim of this study was to explore the regulatory effect of the PGPB Pseudomonas sp. UW4 on growth and physiological indexes of garlic under drought stress. The results revealed that drought stresses significantly reduced total root length, total root surface area, root projection area and total root volume, chlorophyll content, antioxidant enzyme activity and osmolyte content (proline and soluble proteins), and increased relative electrical conductivity and malondialdehyde (MDA) content, all of which could be significantly improved by inoculating the roots with strain UW4. Under drought stress, an increase in total surface area of roots of 87.06% and an increase in root projected area of 40.71% were observed upon inoculation with strain UW4. The a, b, and total content of chlorophyll were increased significantly by 83.63%, 217.33% and 100.02%, respectively. The osmolyte content in leaves significantly increased, and decreased significantly in roots. The content of antioxidants also significantly increased. Moreover, the relative electrical conductivity in leaves and roots was decreased by 23.18% and 41.20%, respectively, upon strain UW4 inoculation. The content of malondialdehyde (MDA) was decreased by 25.23% and 54.08%, respectively, in the presence of strain UW4. The result of principal component analysis (PCA) revealed that the key factors influencing drought tolerance in garlic inoculated with Pseudomonas sp. UW4 could be summarized into two categories: photosynthetic pigments and root growth-related factors, and leaf osmotic adjustment and root antioxidant enzyme-related factors. Based on the result of the Mantel test, it can be inferred that there was a connection between the osmoregulation and antioxidant enzyme systems in the roots and leaves. Based on the D values, the comprehensive evaluation result of drought resistance was that the drought resistance of the garlic inoculated with strain UW4 under drought stress was lower than that of the garlic inoculated with UW4 under normal treatment and higher than that of the garlic under normal treatment. Therefore, Pseudomonas sp. UW4 enhanced the drought resistance of garlic seedlings by improving root phenotype and antioxidant enzyme activity, and increasing the content of shoot chlorophyll. Full article

In this paper, we propose a novel image restoration framework that integrates optimal control techniques with the Hamilton–Jacobi–Bellman (HJB) equation. Motivated by models from production planning, our method restores degraded images by balancing an intervention cost against a state-dependent penalty that quantifies the loss of critical image information. Under the assumption of radial symmetry, the HJB equation is reduced to an ordinary differential equation and solved via a shooting method, from which the optimal feedback control is derived. Numerical experiments, supported by extensive parameter tuning and quality metrics such as PSNR and SSIM, demonstrate that the proposed framework achieves significant improvement in image quality. The results not only validate the theoretical model but also suggest promising directions for future research in adaptive and hybrid image restoration techniques. Full article

Origanum petraeum Danin, an endemic medicinal shrub from Jordan, belongs to the Lamiaceae family and possesses significant pharmaceutical potential, yet its secondary metabolite profile remains largely unexplored. This study evaluated the effects of two types of nanoparticles, silver (Ag) and copper (Cu), on in vitro propagation and secondary metabolite composition in O. petraeum microshoots. Sterilized buds were used to initiate in vitro cultures on Murashige and Skoog (MS) medium supplemented with gibberellic acid (GA₃) at 0.5 mg/L. Microshoots were treated with nanoparticles at concentrations of 0, 25, 50, 100, and 150 mg/L. AgNPs at 100 mg/L promoted growth, increasing the number of microshoots to 11.6 and shoot height to 9.22 cm. Transmission electron microscopy confirmed nanoparticle uptake and translocation, with AgNPs observed in root cells as small particles (≤24.63 nm), while CuNPs formed aggregates in leaves (47.71 nm). GC-MS analysis revealed that nanoparticles altered the volatile composition; 50 mg/L CuNPs enhanced monoterpenes, including α-terpinyl acetate (29.23%) and geranyl acetate (12.76%), whereas 50 mg/L AgNPs increased sesquiterpenes, such as caryophyllene oxide (28.45%). Control in vitro cultures without nanoparticles showed simpler profiles dominated by caryophyllene oxide, while wild plants contained both monoterpenes and sesquiterpenes, with eudesm-7(11)-en-4-ol (25.10%) as the major compound. Nutrient analysis indicated that nanoparticles influenced nutrient composition in microshoots. This study is the first to report nanoparticle-assisted growth and essential oil composition in O. petraeum, demonstrating their potential to enhance growth and secondary metabolite production for pharmacological and biotechnological applications. Full article

Heavy metal pollutants and organic contaminants often co-exist in the environment, posing significant ecological risks due to their combined toxicity. Phytoremediation, a plant-based biotechnology, offers a promising solution for pollutant removal. This study investigated the potential cadmium (Cd) removal capacity of Narrow Crown Black-Cathay poplar (Populus × canadensis Moench × Populus simonii Carr. f. fastigiata Schneid.) under combined Cd-phenol stress. The results showed that the combined stress synergistically inhibited the photosynthetic physiological characteristics, with an inhibition rate up to 54.0%, significantly higher than that under single stress (p < 0.05). Cd accumulation varied markedly among plant organs, following the order: root (ranging from 4000.2 to 9277.0 mg/kg) > stems (ranging from 96.0 to 383.6 mg/kg) > leaf (ranging from 10.3 to 40.1 mg/kg). Phenol enhanced Cd absorption and enrichment in the roots by up to 1.8 times but reduced its translocation to aboveground parts by 37.8–40.0%. Notably, at low Cd concentrations, the Cd removal efficiency under combined stress (26.0%) was substantially higher than under single Cd stress (6.6%). In contrast, biomass, tolerance index, and root–shoot ratio were slightly affected in all treatments (p > 0.05). These findings demonstrate that Narrow Crown Black-Cathay poplar is a suitable candidate for the short-term remediation of Cd in environments co-contaminated with cadmium and phenol. Full article

Gears are the core components of transmission systems, and their health status is critical to the safety and stability of the entire system. In order to efficiently identify the typical fault types such as missing teeth and broken teeth in gears, this paper collects a rich sample under complex backgrounds from different shooting angles and lighting conditions. Then a hierarchical approach is used to describe gear faults on the image. The gear samples are first segmented for image extraction and then finely labeled for gear fault regions. In addition, imbalanced datasets are produced to simulate the environment with fewer fault samples in the actual industrial process. Finally, a semi-supervised learning framework is trained based on the above method and applied in actual environment. The experimental results show that the model performs well in gear target detection and fault diagnosis, demonstrating the effectiveness of the proposed method. Full article

In Santiago, Chile, urban plants are highly vulnerable to drought or climate change. We hypothesize that would find high growth and survival rates in conditions of water scarcity among native species of central Chile. The goal was to determine the effect of the year season and an irrigation gradient on the survival and growth of native plant, in order to evaluate potential plant for use in urban green areas of central Chile. Four plots of 20 m² were located in the Santiago center. In June 2024 twelve species were planted and from November 2024 to March 2025 were irrigated with 13.3, 10.1, 1.7 and 1.4 L/m²/day. The GLM and Kaplan–Meier survival analyses were used. Shoot growth rate was highly variable among species, among irrigation treatments applied, and among year seasons. Eight species showed water-related growth and shoot growth during the winter was very small and higher in spring. Two species showed evidence of water-related survival; in the other 10 species, no significant differences were found between irrigation treatments. Winter was the season with the highest survival rates for eleven species. In conclusion, the results suggest that native plants can achieve high survival rates with limited irrigation. This highlights their potential for use in the urban area in Mediterranean-type climates where rainfall is expected to be low due to climate change. Full article

The germination, seedling growth, and crop productivity of maize seeds are significantly impacted by seed aging. This study investigated the efficacy of silver nanoparticles (AgNPs) as a seed priming agent for maize inbred lines exhibiting varying degrees of aging tolerance. Two inbred lines, aging-sensitive I178 and aging-tolerant X178, were used. AgNP treatment significantly promoted the germination of I178 (from 55% to 85%, compared with water treatment). Notable improvements were observed in root length, shoot length, and lateral root formation after AgNP treatment in I178. However, X178 showed no significant changes in germination and seedling growth after the AgNP treatment. Further transcriptomic analysis was performed on X178 and I178 before (water treatment) and after AgNP treatment to study genes and the expression network of the mechanism induced by AgNP promotion. In I178, AgNP treatment led to a substantial increase in differentially expressed genes (DEGs). A total of 800 DEGs were identified, with 517 being upregulated and 283 downregulated. The DEGs in I178 were mainly involved in metabolic processes, stress responses, and membrane repair. For example, genes related to lipid metabolism and membrane integrity were upregulated, along with seven genes associated with antioxidant action and redox metabolism. This indicates that AgNPs might enhance membrane stability and stress tolerance in I178. In contrast, X178 had a limited transcriptomic response to AgNP treatment. Although 874 DEGs were detected, the number of genes related to key processes like those in I178 did not change significantly, which is in line with its inherent aging tolerance. Overall, these results suggest that AgNPs can effectively improve seed vigor and counteract the negative effects of seed aging, especially in aging-sensitive maize lines. The mechanism likely occurs through regulating gene expression related to stress response, metabolic repair, and membrane stability. This study provides new insights into the molecular basis of AgNP-mediated seed vigor enhancement, which has potential implications for improving seed quality in agricultural production. Full article

Bamboo (subfamily Bambusoideae, Poaceae) ranks among the fastest-growing plants on Earth, achieving up to 1 m day⁻¹, significantly faster than other fast growing woody plant such as Eucalyptus (up to 0.6 m day⁻¹) and Populus (up to 0.5 m day⁻¹). Native to Asia, South America and Africa, and cultivated on approximately 37 million ha worldwide, bamboo delivers multifaceted environmental, social, and economic benefits. Historically central to construction, handicrafts, paper and cuisine, bamboo has evolved into a high-value cash crop and green innovation platform. Its rapid renewability allows multiple harvests of young shoots in fast-growing species such as Phyllostachys edulis and Dendrocalamus asper. Its high tensile strength, flexibility, and ecological adaptability make it suitable for applications in bioenergy (bioethanol, biogas, biochar), advanced materials (engineered composites, textiles, activated carbon), and biotechnology (fermentable sugars, prebiotics, biochemicals). Bamboo shoots and leaves provide essential nutrients, antioxidants and bioactive compounds with documented health and pharmaceutical potential. With a global market value exceeding USD 41 billion, bamboo demand continues to grow in response to the call for sustainable materials. Ecologically, bamboo sequesters up to 259 t C ha⁻¹, stabilizes soil, enhances agroforestry systems and enables phytoremediation of degraded lands. Nonetheless, challenges persist, including species- and age-dependent mechanical variability; vulnerability to decay and pests; flammability; lack of standardized harvesting and engineering codes; and environmental impacts of certain processing methods. This review traces bamboo’s trajectory from a traditional resource to a strategic bioresource aligned with Industry 5.0, underscores its role in low-emission, circular bioeconomies and identifies pathways for optimized cultivation, green processing technologies and integration into carbon-credit frameworks. By addressing these challenges through innovation and policy support, bamboo can underpin resilient, human-centric economies and drive sustainable development. Full article

Reducing nitrogen (N) fertilizer input while sustaining maize yield and alleviating nitrogen leaching is a significant challenge due to economic and practical feasibility, as well as the environmental friendliness of this process. However, it remains unclear whether reducing nitrogen by using a blend of slow/controlled-release nitrogen fertilizer (SCRNF) with urea at an equal nitrogen rate can achieve the desired yield and mitigate nitrogen leaching. A field experiment consisting of four treatments (240 kg·N·hm⁻², 100% urea, CK; 240 kg·N·hm⁻², 50% N from urea and 50% N from SCRNF, N100%; 192 kg·N·hm⁻², 50% N from urea and 50% N from SCRNF under 20% N reduction, NR20%; 144 kg·N·hm⁻², 50% N from urea and 50% N from SCRNF under 40% N reduction, NR40%) was conducted in Shanxi from 2019 to 2021. In this study, we explored the effects of a mixture of SCRNF and urea on grain yield, yield components, main agronomic traits, nitrogen partial factor productivity, and content of nitrate/ammonium nitrogen in soil in maize under decreasing amounts of nitrogen fertilization. The results showed that the mixture of SCRNF and urea can improve spring maize yield under reduced nitrogen input, with its yield and yield component factors generally performing better than those of the control. The yield of the NR20% treatment was highest in 2020 and 2021, increasing by 8.8% and 11.7% over CK, respectively; the NR20% and NR40% treatments had no significant impact on the main agronomic traits of spring maize, such as plant height, leaf area, shoot biomass, and SPAD value of the ear leaf, compared with CK; the NR20% and NR40% treatments significantly (p < 0.05) enhanced nitrogen partial factor productivity but reduced nitrate and ammonium nitrogen in 0~200 cm soil over the three years compared with CK. Therefore, reducing nitrogen input by 20% with 50% N from urea and 50% N from biodegradable film-coated urea was an appropriate nitrogen fertilizer management measure for mitigating environmental risks without compromising maize yield in North China. Full article

Jujube (Ziziphus jujuba Mill.) is an important economic fruit tree in China, and its in vitro culture technology is the key to achieving large-scale seedling cultivation. PGRs (Plant growth regulators) play a central regulatory role in all stages of jujube micropropagation, including explant initiation, proliferation, and rooting. This article provides a comprehensive overview of recent advances in in vitro culture of jujube, with a focus on the recommended exogenous phytohormone ratios, their effects, and underlying regulatory mechanisms across distinct varieties during the key stages such as in vitro culture, shoot proliferation, and root formation. The primary culture of most jujube varieties usually employs the MS medium, and it is recommended that auxin and cytokinin be used in combination. During the initial cultivation stage, the use of NAA (1-naphthaleneacetic acid) or IBA (indole butyric acid) is recommended at concentrations ranging from 0.1 to 1.0 mg/L. At the same time, 6-BA (6-benzylaminopurine) is suggested, with a concentration range of 0.5 to 2.5 mg/L. In the subculture multiplication of most jujube varieties, MS medium is used, and auxin (such as NAA, IBA), and TDZ (thidiazuron) and cytokinin (e.g., 6-BA) are used in combination. The recommended concentration range for auxin remains between 0.1 and 1.0 mg/L, and for cytokinin 6-BA between 0.5 and 2.5 mg/L, while the recommended concentration of TDZ is suggested to be below 0.01 mg/L. Rooting induction for most jujube varieties has predominantly been achieved using 1/2 MS medium, with growth regulator concentrations typically ranging from 0.5 to 3.0 mg/L. Full article