Search Results (151)

Rapid pressure fluctuations—known as hydraulic transients—occur during valve operations or load changes in turbines and pumps. The presence of biofouling, particularly caused by the golden mussel (Limnoperna fortunei), can intensify these effects and compromise the structural integrity of pressurized systems. This study experimentally evaluated the influence of such biofouling on pressure peaks during transient events in forced conduits. A hydraulic test rig was developed using PVC pipes with nominal diameters of 2½”, 3”, and 4”, tested under both clean conditions and with simulated biofouling printed in 3D, replicating mussel morphology. Results showed that, under the same initial flow rates, pressure peaks in biofouled pipes were significantly higher than in clean ones, especially in smaller diameters. To mitigate structural risks, the downstream shut-off valve closure time was modulated using a needle valve, effectively reducing peak pressures to levels closer to design limits. It is concluded that L. fortunei colonization alters transient hydraulic behavior and should be considered in the design and operation of systems vulnerable to biofouling, particularly in critical infrastructure such as water supply networks and hydroelectric power plants. Full article

In South Korea, securing ground space for installing hydrogen refueling stations in urban areas is challenging due to limited ground space and high-density development. Safety concerns for hydrogen systems in enclosed urban environments also require careful consideration. To address this issue, this study explored a method of undergrounding hydrogen infrastructure as a solution for urban hydrogen charging stations. This study examined the characteristics of hydrogen diffusion and concentration reduction under leakage conditions within a confined hydrogen infrastructure, focusing on key safety systems, including emergency shut-off valves (ESVs) and ventilation fans. We discovered that the ESV reduced hydrogen concentration by over 80%. Installing two or more ventilation fans arranged horizontally improves airflow and enhances ventilation efficiency. Moreover, increasing the number of fans reduces stagnant zones within the space, effectively lowering the average hydrogen concentration. Full article

While IPV is often studied as a predictor of housing insecurity, few U.S. studies explore how different forms of housing instability may contribute to intimate partner violence (IPV) risk. Using a mixed-methods approach and a cross-sectional design, this study examined the association between four housing instability domains and IPV among a sample of tenants that had either experienced eviction or were at high risk for eviction. Tenants in Harris and Travis counties (Texas, USA) completed an online survey (n = 1085; March–July 2024). Housing instability was assessed across four domains: homelessness, lease violations, utility hardship, and poor housing quality. IPV was measured using the Hurt, Insult, Threaten, Scream Screener. Covariate-adjusted logistic regression models suggest indicators within the four housing instability domains were associated with IPV risk. Within the homelessness domain, experiences with lifetime homelessness (AOR = 1.92, 95%CI 1.61–2.28), in the past 12 months living in unconventional spaces (AOR = 2.10, 95%CI 1.92–2.29), and moving in with others (AOR = 1.20, 95%CI 1.06–1.36) were associated with IPV. Within the lease violations domain, missed rent payments (AOR = 1.69, 95%CI 1.68–1.71) and non-payment lease violations (AOR = 2.50, 95%CI 2.29–2.73) in the past 12 months were associated with IPV. Utility shutoffs (AOR = 1.62, 95%CI 1.37–1.91) and unsafe housing (AOR = 1.65, 95%CI 1.31–2.09) in the past 12 months were associated with IPV. Homelessness, housing-related economic hardships and substandard living conditions predict an elevated risk of IPV. Full article

This study focuses on the optimization of valve assemblies in downhole rod pumping units (DRPUs), which remain the predominant artificial lift technology in oil production worldwide. The research addresses the critical issue of premature failures in DRPUs caused by leakage in valve pairs, i.e., a problem that accounts for approximately 15% of all failures, as identified in a statistical analysis of the 2022 operational data from the Uzen oilfield in Kazakhstan. The leakage is primarily attributed to the accumulation of mechanical impurities and paraffin deposits between the valve ball and seat, leading to concentrated surface wear and compromised sealing. To mitigate this issue, a novel valve assembly design was developed featuring a flow turbulizer positioned beneath the valve seat. The turbulizer generates controlled vortex motion in the fluid flow, which increases the rotational frequency of the valve ball during operation. This motion promotes more uniform wear across the contact surfaces and reduces the risk of localized degradation. The turbulizers were manufactured using additive FDM technology, and several design variants were tested in a full-scale laboratory setup simulating downhole conditions. Experimental results revealed that the most effective configuration was a spiral plate turbulizer with a 7.5 mm width, installed without axis deviation from the vertical, which achieved the highest ball rotation frequency and enhanced lapping effect between the ball and the seat. Subsequent field trials using valves with duralumin-based turbulizers demonstrated increased operational lifespans compared to standard valves, confirming the viability of the proposed solution. However, cases of abrasive wear were observed under conditions of high mechanical impurity concentration, indicating the need for more durable materials. To address this, the study recommends transitioning to 316 L stainless steel for turbulizer fabrication due to its superior tensile strength, corrosion resistance, and wear resistance. Implementing this design improvement can significantly reduce maintenance intervals, improve pump reliability, and lower operating costs in mature oilfields with high water cut and solid content. The findings of this research contribute to the broader efforts in petroleum engineering to enhance the longevity and performance of artificial lift systems through targeted mechanical design improvements and material innovation. Full article

Coronavirus non-structural protein 1 (nsp1) is a pathogenic determinant of Betacoronaviruses. Previous studies demonstrated that the nsp1 of various coronaviruses induces host shutoff through a variety of mechanisms; however, there is little information on the function of bovine coronavirus (BCoV) nsp1. We aimed to characterize the host gene expression suppression function of BCoV nsp1. We first confirmed that the expression of BCoV nsp1 in MAC-T cells, a bovine mammary epithelial cell line, suppressed host and reporter gene expression. Subsequently, lysine and phenylalanine at amino acid positions 232 and 233, respectively, were identified as key residues required for this suppressive effect. Expression levels of housekeeping genes are comparable in cells expressing wild-type BCoV nsp1 and a mutant with alanine substitutions at positions 232 and 233 (BCoV nsp1-KF). Wild-type BCoV nsp1 localized to both the cytoplasm and nucleus; however, BCoV nsp1-KF exhibited prominent nuclear accumulation with dot-like structures. Using confocal microscopy and co-sedimentation analysis, we identified an association between wild-type BCoV nsp1, but not BCoV nsp1-KF, and ribosomes, suggesting that ribosome binding is required for BCoV nsp1-mediated suppression of host gene expression. This is the first study of the characterization of host gene expression suppression by BCoV nsp1. Full article

This study aims to assess the carbon footprint for the organization of frozen processed seafood manufacturing plants and propose sustainable strategies for reducing greenhouse gas emissions. Organizational activity data from 2024 were utilized to evaluate the carbon footprint and develop targeted mitigation measures. The findings indicate that Scope 1 emissions amounted to 12,685 tons of CO₂eq, Scope 2 emissions amounted to 15,403 tons of CO₂eq, and Scope 3 emissions amounted to 31,564 tons of CO₂eq. The total greenhouse gas emissions across all three scopes were 59,652 tons of CO₂eq, with additional greenhouse gas emissions recorded at 34,027 tons of CO₂eq. Mitigation measures were considered for activities contributing to at least 10% of emissions in each scope. In Scope 1, the use of R507 refrigerant in the production cooling system accounted for 9907 tons of CO₂eq, representing 78.10% of emissions. In Scope 2, electricity consumption contributed 15,403 tons of CO₂eq, constituting 100% of emissions. In Scope 3, the procurement of surimi (processed fish meat) was responsible for 20,844 tons of CO₂eq, accounting for 66.04% of emissions. Based on these findings, key mitigation strategies were proposed. For Scope 1, reducing emissions involves preventive maintenance of cooling systems to prevent leaks, replacing corroded pipelines, installing shut-off valves, and switching to alternative refrigerants with no greenhouse gas emissions. For Scope 2, energy-saving initiatives include promoting electricity conservation within the organization, maintaining equipment for optimal efficiency, installing energy-saving devices such as variable speed drives (VSD), upgrading to high-efficiency motors, and utilizing renewable energy sources like solar power. For Scope 3, emissions can be minimized by sourcing raw materials from suppliers with certified carbon footprint labels, prioritizing purchases from producers committed to carbon reduction, and selecting suppliers closer to manufacturing sites to reduce transportation-related emissions. Implementing these strategies will contribute to sustainable greenhouse gas emission reductions. Full article

This research aims to calculate the Carbon Footprint for Organization of a plant manufacturing frozen processed seafood and propose strategies to reduce greenhouse gas (GHG) emissions following the Net-Zero Pathway, using 2024 as the baseline year. The findings indicate that Scope 1 emissions amounted to 12,685 tons of CO₂ eq, Scope 2 emissions totaled 15,403 tons of CO₂eq, and Scope 3 emissions reached 31,564 tons of CO₂eq, leading to a combined total of 59,652 tons of CO₂eq across all scopes, with an additional 34,027 tons of CO₂eq from other GHG sources. To achieve net-zero emissions by 2050, annual reductions of 3.46% per category are required. The short-term target for 2028f aims to reduce emissions to 10,929 tons of CO₂eq for Scope 1, 13,270 tons of CO₂eq for Scope 2, and 27,194 tons of CO₂eq for Scope 3, resulting in total emissions of 51,392 tons of CO₂eq. The proposed reduction strategies include optimizing Scope 1 emissions by preventing leaks in R507 refrigerant systems, replacing corroded pipelines, installing shut-off valves, and switching to low-GHG refrigerants. For Scope 2, measures focus on reducing electricity consumption through energy conservation initiatives, carrying out regular machinery maintenance, installing Variable Speed Drives (VSDs), upgrading to high-efficiency motors, and integrating renewable energy sources such as solar power. For Scope 3, emissions from raw material procurement can be minimized by sourcing from certified suppliers with established product carbon footprints, prioritizing carbon reduction labeling, and selecting nearby suppliers to reduce transportation-related emissions. These strategies will support the organization in achieving carbon neutrality and progressing toward the net-zero goal. Full article

The development of shale gas relies on hydraulic fracturing technology and requires the injection of a large amount of fracturing fluid. The well shut-off period after fracturing can promote water infiltration and suction. Optimizing the well shut-off time is crucial for enhancing the recovery rate. Among existing methods, the dimensionless time model is widely used, but it has limitations because it does not represent the length of on-site scale features. In this study, we focused on the shut-in time for a deep shale gas well (Lu-A) in Luzhou and a medium-deep shale gas well (Wei-B) in Weiyuan. By integrating the spontaneous seepage and aspiration experiments in the laboratory and the post-pressure backflow data (including mineralization degree, liquid volume recovery rate, etc.), a multi-scale well shutdown time prediction model considering the characteristic length was established. The experimental results show that the spontaneous resorption characteristic times of Lu-A and Wei-B are 3 h and 22 h, respectively. Based on the inversion of crack monitoring data, the key parameters such as the weighted average crack width (1.73/1.30 mm) and crack spacing (0.20/0.32 m) of Lu-A and Wei-B were obtained. Through the scale upgrade calculation of the feature length (0.10/0.16 m), the system determined that the optimal well shutdown times for the two wells were 14.5 days and 16.7 days, respectively. The optimization method based on a multi-parameter analysis of backflow fluid proposed in this study not only solves the limitations of the traditional dimensionless time model in characterizing the feature length but also provides a theoretical basis for the formulation of the well shutdown system and nozzle control strategy of shale gas wells. Full article

The challenge of water shutoff in carbonate reservoirs is complicated by the presence of fractures, which cannot be effectively blocked using conventional hydrogel screens designed for granular reservoirs. To reliably seal fractures, fibrous and dispersed fillers are added to hydrogels. These fillers must exhibit affinity for the matrix to ensure the composites can effectively isolate water. Given the wide variability in fracture apertures, it is evident that water shutoff composites should incorporate fibers and dispersed fillers of varying geometric sizes. This study presents a range of hydrogel composites reinforced with mono-, bi-, and tri-component fibers, as well as dispersed fillers, designed for water shutoff in fractured carbonate reservoirs with varying fracture apertures. Oscillation test results demonstrated a twofold increase in the elastic modulus (40–45 Pa) for compositions with various fillers compared to the base composition (23 Pa). Filtration studies revealed the effectiveness of the optimized compositions under different fracture apertures. Specifically, even at a fracture aperture of 650 μm, the residual resistance factor (RRF) reached 82.3 and 9.76 at water flow rates of 0.1 cm³/min and 0.5 cm³/min, respectively. The conducted rheological and filtration tests, along with field trials, confirmed the validity of the selected approach. Full article

In the field of enhanced oil recovery (EOR), particularly for water control in high-temperature reservoirs, there is a critical need for effective in-depth water shutoff and conformance control technologies. Polymer-based in situ-cross-linked gels are extensively employed for enhanced oil recovery (EOR), yet their short gelation time under high-temperature reservoir conditions (e.g., >120 °C) limits effective in-depth water shutoff and conformance control. To address this, we developed a hydrogel system via the in situ cross-linking of polyacrylamide (PAM) with phenolic resin (PR), reinforced by silica sol (SS) nanoparticles. We employed a variety of research methods, including bottle tests, viscosity and rheology measurements, scanning electron microscopy (SEM) scanning, density functional theory (DFT) calculations, differential scanning calorimetry (DSC) measurements, quartz crystal microbalance with dissipation (QCM-D) measurement, contact angle (CA) measurement, injectivity and temporary plugging performance evaluations, etc. The composite gel exhibits an exceptional gelation period of 72 h at 130 °C, surpassing conventional systems by more than 4.5 times in terms of duration. The gelation rate remains almost unchanged with the introduction of SS, due to the highly pre-dispersed silica nanoparticles that provide exceptional colloidal stability and the system’s pH changing slightly throughout the gelation process. DFT and SEM results reveal that synergistic interactions between organic (PAM-PR networks) and inorganic (SS) components create a stacked hybrid network, enhancing both mechanical strength and thermal stability. A core flooding experiment demonstrates that the gel system achieves 92.4% plugging efficiency. The tailored nanocomposite allows for the precise management of gelation kinetics and microstructure formation, effectively addressing water control and enhancing the plugging effect in high-temperature reservoirs. These findings advance the mechanistic understanding of organic–inorganic hybrid gel systems and provide a framework for developing next-generation EOR technologies under extreme reservoir conditions. Full article

The papers introduced in the Commentary present new insights and review aspects of current knowledge concerning the competition between viruses and their hosts for the cellular translation apparatus. Viruses depend on this apparatus and utilize diverse mechanisms to usurp it for the translation of viral mRNAs and to suppress synthesis of cellular proteins. Virus-induced modification of translation factors, selective abrogation of mRNA binding to ribosomes and degradation of cellular mRNAs all impair elements of the innate immune response, thereby undermining host defenses against infection. Various cellular mechanisms prevent translation of viral mRNAs, by modifying components of the translation apparatus to effect a generalized shut-off of translation or by binding of host proteins to viral mRNAs to induce their degradation or to prevent their engagement with the translation apparatus. Viruses have in turn evolved countermeasures to evade these defenses, for example by encoding proteins that impair the activity of host factors or via alterations in the sequence and structure of viral mRNAs. Such changes enable viral mRNAs to avoid recognition by host factors or to support translation initiation by specialized mechanisms that involve only a subset of the factors that are required by cellular mRNAs. Full article

This study addresses the water hammer protection challenges in the JH gravity-fed bifurcated pipeline network system in Xinjiang, China. A hydraulic transient numerical model is developed using the one-dimensional method of characteristics and implemented in Bentley HAMMER software to systematically analyze the transient response characteristics under different valve closure schemes, with a focus on revealing pressure fluctuation patterns in branch and main pipelines under various shutdown modes. Key findings include the following: Single-valve linear slow closure reduces the maximum water hammer pressure by 54.7%, while the two-stage closure strategy suppresses pressure extremes below safety thresholds with 73.1% higher efficiency than linear closure. For multi-valve conditions, although two-stage closure eliminates negative pressure risks, most of nodes exhibit transient overpressure exceeding 1.5 times the working pressure. By integrating overpressure relief valves into a composite protection system, the maximum transient pressure is strictly controlled within 1.5× rated pressure, and the minimum pressure remains above −2 mH₂O, successfully resolving protection challenges in this complex network. These results provide technical guidelines for the safe operation of gravity-fed pipeline systems in high-elevation-difference regions. Full article

This study presents the formulation and evaluation of a dual molecular weight polymer gel system composed of partially hydrolyzed polyacrylamide (HPAM) and crosslinked with polyethyleneimine (PEI) for water shut-off applications. A soft gel, designed for deep reservoir penetration, was formulated using 5000 ppm high-molecular-weight HPAM, while a rigid gel for near-wellbore blockage combined 5000 ppm high- and 5000 ppm low-molecular-weight HPAM. The gel system was designed at 65 °C, with an initial gelation time exceeding 8 h and viscosity values below 15 cP before gelation, ensuring ease of injection. Laboratory assessments included bottle testing, rotational and oscillatory rheological measurements, and core flooding to determine residual resistance factors (RRFs). The soft gel achieved a final strength of Grade D (low mobility), while the rigid gel reached Grade G (moderate deformability, immobile), according to Sydansk’s classification. RRF values reached 93 for the soft gel and 185 for the rigid gel, with both systems showing strong washout resistance and water shut-off efficiencies above 95%. These results demonstrate the potential of the HPAM/PEI gel system as an effective solution for conformance control in mature reservoirs with active aquifers. Full article

To address the rapid crosslinking reaction and short stability duration of polyacrylamide gel under high salinity and temperature conditions, this paper proposes utilizing urea to delay the nucleophilic substitution crosslinking reaction among polyacrylamide, hydroquinone, and formaldehyde. Additionally, urea regulates the precipitation of calcium and magnesium ions, enabling the in situ preparation of an organic/inorganic composite gel consisting of crosslinked polyacrylamide and carbonate particles. With calcium and magnesium ion concentrations at 6817 mg/L and total salinity at 15 × 10⁴ mg/L, the gelation time can be controlled to range from 6.6 to 14.1 days at 95 °C and from 2.9 to 6.5 days at 120 °C. The resulting composite gel can remain stable for up to 155 days at 95 °C and 135 days at 120 °C. The delayed gelation facilitates longer-distance diffusion of the gelling agent into the formation, while the enhancements in gel strength and stability provide a solid foundation for improving the effectiveness of profile control and water shut-off in oilfields. The urea-controlling method is novel and effective in extending the high-temperature cross-linking reaction time of polyacrylamide. By converting calcium and magnesium ions into inorganic particles, it enables the in situ preparation of organic/inorganic composite gels, enhancing their strength and stability. Full article

During natural gas development, the gas–water two-phase flows in upward and horizontal wellbores are complex and variable. The accurate calculation of the water holdup in each production layer using appropriate methods based on the logging data collected by fluid identification instruments can enable the precise identification of primary oil-producing and water-producing layers and facilitate subsequent water shutoff operations. In this study, we first investigated the measurement techniques and calculation methods for gas–water two-phase holdups both in China and internationally. Second, we conducted gas–water two-phase simulation experiments in upward and horizontal large-diameter wellbores using a Triangular Arm Array Imager (TAAI) equipped with six fiber-optic probes in a multiphase flow simulation laboratory. We then categorized the flow patterns observed in the physical simulation experiments based on typical theoretical classifications of gas–water two-phase flow patterns. Subsequently, we calculated the spatial positions of the fiber-optic probes and the local water holdup in the wellbore cross-section from the data collected by TAAI and compared the results obtained by Gaussian radial basis function (GRBF) or inverse distance weighted (IDW) interpolation algorithms. We processed the experimental data and found significant discrepancies between the holdup calculated by the two algorithms and the actual wellbore holdup. Therefore, we applied the Levenberg–Marquardt (L-M) algorithm to optimize these interpolation algorithms and discovered that the holdup obtained from the optimized algorithms aligned more closely with the actual wellbore holdup with reduced errors. Finally, we applied the optimized algorithms to the processing of measured data from a gas–water two-phase horizontal well. The results indicate that the L-M algorithm can improve the accuracy of 4–5% of holdup calculations. In the actual production process, the output situation of each production layer can be more accurately judged to provide important opinions for the subsequent actual production by this study. Full article