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41 pages, 2956 KB  
Review
Sustainable Environmental Analysis of Soil, Water, and Machine Interactions: A Review
by Mohamed Ghonimy, Ahmed M. Aggag, Ahmed Alzoheiry and Abdulaziz Alharbi
Sustainability 2026, 18(6), 2900; https://doi.org/10.3390/su18062900 - 16 Mar 2026
Abstract
Sustainable agriculture in arid and semi-arid regions critically depends on the interactions between soil physical properties, water dynamics, and mechanized field operations. In this context, soil physical attributes, such as texture, bulk density, aggregate stability, and soil water potential, play a crucial role [...] Read more.
Sustainable agriculture in arid and semi-arid regions critically depends on the interactions between soil physical properties, water dynamics, and mechanized field operations. In this context, soil physical attributes, such as texture, bulk density, aggregate stability, and soil water potential, play a crucial role in determining soil–water–machine interactions. Soil attributes such as texture, bulk density, aggregate stability, and soil water potential govern both water movement and retention, as well as traction efficiency, draft energy, and compaction under mechanized traffic. Deviations from the optimal soil moisture range in sandy or calcareous soils increase wheel slip, energy consumption, and soil structural degradation, resulting in uneven infiltration and reduced water-use efficiency. This review synthesizes recent research on these coupled processes, emphasizing how soil mechanics and hydraulics collectively influence irrigation performance and mechanization energy requirements. The novelty of this study lies in presenting an integrated soil–machine–water conceptual framework that captures the continuous interactions and interdependencies among soil physical state, machine behavior, and water movement. By highlighting these dynamic relationships, this review provides a systems-level perspective on energy and water interactions in dryland agroecosystems, offering a foundation for predicting the environmental implications of mechanized operations under arid conditions. Overall, the review demonstrates that sustainable mechanized agriculture in arid regions requires integrated management of soil physical state, machine operation, and irrigation timing, where maintaining soil moisture within an optimal operational range is the key factor for reducing energy losses, preventing soil compaction, and improving water productivity. Full article
(This article belongs to the Special Issue Sustainable Environmental Analysis of Soil and Water—2nd Edition)
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18 pages, 1644 KB  
Article
Evaluation of the Cytotoxicity of Biochar Aqueous Extract in Caco-2 Cells: Time-Dependent Regulation of Apoptosis, Associated with miRNA Modulation
by Sidra Amin, Klaudia Marcinkowska, Magdalena Wołoszyńska, Sebastian Opaliński, Dawid Skrzypczak, Paweł Wiercik, Łukasz Bobak and Agnieszka Śmieszek
Molecules 2026, 31(6), 989; https://doi.org/10.3390/molecules31060989 - 16 Mar 2026
Abstract
Biochar, a carbon-rich material traditionally used to improve soil health and as a feed additive, has recently attracted attention for its potential biological activity. This study examined the effects of an aqueous biochar extract (BC-AE) on human intestinal epithelial cells (Caco-2), focusing on [...] Read more.
Biochar, a carbon-rich material traditionally used to improve soil health and as a feed additive, has recently attracted attention for its potential biological activity. This study examined the effects of an aqueous biochar extract (BC-AE) on human intestinal epithelial cells (Caco-2), focusing on its influence on cell viability and apoptosis. The metabolic activity of Caco-2 cells exposed to BC-AE was first evaluated using an MTS assay. A concentration of 3 mg/mL, which promoted Caco-2 metabolic activity, was selected for further testing at 24 and 72 h. The effect of BC-AE on cell viability was assessed by epifluorescence microscopy (morphology) and flow cytometry (apoptosis profiling). The transcriptional response of cell viability-related genes (BAX, BAD, BCL-2, BCL-xL, MCL-1, P21, and P53) and microRNAs (miR-15b, miR-19, miR-21, miR-33a, miR-155, and miR-486) was analyzed by RT-qPCR. In parallel, selected proteins (BAD, BAX, BCL-2, and MCL-1) were examined by Western blotting. We showed that BC-AE decreased cell viability after 24 h via late apoptosis, while 72 h exposure increased necrosis without further viability loss. Both BAX and MCL-1 protein levels increased in Caco-2 cells after 72 h of BC-AE treatment, and miR-15b and miR-21 were upregulated, suggesting the involvement of a regulatory mechanism controlling cell survival. The obtained findings highlight the importance of considering both concentration and exposure duration when assessing biochar bioactivity and represent an additional contribution to the ongoing effort to better understand the biological role. Full article
(This article belongs to the Special Issue Exploring Bioactive Compounds in Foods and Nutrients for Human Health)
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29 pages, 16838 KB  
Article
Spatiotemporal Evolution of Drought and Its Multi-Factor Driving Mechanisms in Xinjiang During 1981–2020
by Xuchuang Yu, Siguo Liu, Anni Deng, Runsen Li, Xiaotao Hu, Ping’an Jiang and Ning Yao
Agriculture 2026, 16(6), 669; https://doi.org/10.3390/agriculture16060669 - 15 Mar 2026
Abstract
Drought is a highly destructive natural disaster that inflicts severe economic losses. Its formation mechanisms are complex, yet existing studies have often focused on single driving factors, leaving the synergistic effects of multiple factors insufficiently explored. Based on multi-source data from Xinjiang spanning [...] Read more.
Drought is a highly destructive natural disaster that inflicts severe economic losses. Its formation mechanisms are complex, yet existing studies have often focused on single driving factors, leaving the synergistic effects of multiple factors insufficiently explored. Based on multi-source data from Xinjiang spanning 1981–2020, this study systematically examined the combined impacts of atmospheric circulation, underlying surface conditions, and human activities on drought, using the multi-temporal-scale Standardized Precipitation Evapotranspiration Index (SPEI) and Standardized Soil Moisture Index (SSI), along with partial correlation analysis, spatial autocorrelation, and principal component analysis. The results show that Xinjiang experienced a pronounced drying trend over the past 40 years, with the seasonal SPEI and SSI both exhibiting significant declines. Drought intensity was higher in northern Xinjiang than in the south. Correlations between drought indices and circulation indices, such as Atlantic Multidecadal Oscillation (AMO), were relatively weak, indicating a limited regulatory influence of large-scale circulation on regional drought under the dual constraints of topography and an inland setting. Among underlying surface factors, slope significantly influenced drought spatial patterns. Mountainous areas and basin interiors showed positive spatial correlations, characterized respectively by high–high clustering (high slope and high drought index) and low–low clustering (low slope and low drought index). In contrast, basin margins exhibited low–high clustering (low slope surrounded by high drought index), reflecting negative spatial correlation. Aspect showed no significant effect. Vegetation cover displayed clear seasonal coupling with drought, with strong negative correlations in spring due to intensified water stress. Human activities also played a prominent role. Since the mid-1990s, the expansion of built-up land and increased agricultural water use have shifted drought–land use relationships toward low–high clustering (low drought index surrounded by high land-use intensity) in southern Xinjiang oases, and toward low–low clustering (low drought index and low land-use intensity) in eastern Xinjiang. Meanwhile, ecological restoration projects promoted a transition from low–high to high–high clustering (high drought index and high land-use intensity) in some areas, alleviating local drying trends. Principal component analysis further revealed a shift in the dominant driver: land-use change was the primary factor before 2005, whereas vegetation cover became the key driver thereafter. By clarifying the mechanisms underlying multi-factor interactions in drought in Xinjiang, this study provides scientific support for integrated water resource management, ecological conservation, and climate adaptation strategies in arid regions. Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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27 pages, 3861 KB  
Review
Mechanization and Intelligent Technologies for Ginger Harvesting: Evolution, Frontiers, and Prospects
by Haiyang Shen, Guangyu Xue, Gongpu Wang, Wenhao Zheng, Lianglong Hu, Yanhua Zhang and Baoliang Peng
AgriEngineering 2026, 8(3), 112; https://doi.org/10.3390/agriengineering8030112 - 15 Mar 2026
Abstract
Driven by agricultural labor shortages and rising quality requirements, ginger harvesting increasingly demands high-throughput, low-damage operations and a reliable supply chain. This review summarizes harvesting modes and harvester types used in ginger production, with emphasis on critical process modules: digging and lifting, soil [...] Read more.
Driven by agricultural labor shortages and rising quality requirements, ginger harvesting increasingly demands high-throughput, low-damage operations and a reliable supply chain. This review summarizes harvesting modes and harvester types used in ginger production, with emphasis on critical process modules: digging and lifting, soil disintegration and cleaning, vine cutting and anti-tangling, gentle conveying, and collection. We compare major technical routes in terms of field capacity, control of soil and foreign materials, damage mitigation, and reliability under continuous operation, and identify the conditions under which each route performs best. Drawing on advances in harvesting systems for other root and bulb crops, we outline transferable approaches for intelligent sensing, precision control, and system-level integration. We then propose an online monitoring and closed-loop regulation framework for strongly coupled conditions, such as heavy clay soils, plastic-mulch residues, and vine interference. Key bottlenecks include limited cross-regional adaptability, persistent trade-offs between low damage and high throughput, cost constraints on intelligent functions, and the lack of shared datasets and standardized evaluation protocols. Future progress should be anchored in integrated equipment sets and supporting operating specifications, guided by multi-source sensing-based quality indicators and interpretable control strategy libraries, to reduce harvest losses, stabilize marketable quality, improve operational efficiency, and enable scalable adoption. Full article
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27 pages, 7601 KB  
Article
Hydrological Modeling of Reservoir Sedimentation and Evolution of Elevation–Capacity Curve of the Dam Reservoir
by Baradin Adisu Arebu, Nassir Alamri and Amro Elfeki
Hydrology 2026, 13(3), 93; https://doi.org/10.3390/hydrology13030093 - 13 Mar 2026
Viewed by 60
Abstract
Accurate modeling of dam reservoir sedimentation is crucial for effective reservoir management. Traditional approaches for estimating sedimentation include the Hydraulic Approach (HA) and the Empirical Approach (EA). HA involves complex computations and requires substantial data, while the EA relies on equations like the [...] Read more.
Accurate modeling of dam reservoir sedimentation is crucial for effective reservoir management. Traditional approaches for estimating sedimentation include the Hydraulic Approach (HA) and the Empirical Approach (EA). HA involves complex computations and requires substantial data, while the EA relies on equations like the Universal Soil Loss Equation (USLE) and the Revised Universal Soil Loss Equation (RUSLE), which use subjective parameters and lead to inaccurate estimations. This study introduces a novel approach called the hydrological approach, which integrates the sediment rating curve (SRC) and the dam reservoir elevation-capacity curve (ECC) to estimate reservoir sedimentation and evolution of the ECC. This HA leads to a newly developed equation for the estimation of the sediment rise and the corresponding sediment volume. The approach is applied to the Wadi Fatimah Dam in Saudi Arabia. By combining rainfall data from 1985 to 2022 and performing rainfall–runoff hydrological modeling combined with the proposed HA, sediment accumulation trends and reservoir capacity reductions are estimated from past to present. Validation through ground survey and geophysical investigations in 2008 confirms model accuracy. Findings reveal significant sediment buildup, with an estimated average of 7.5 m rise from 1985 to 2008. The study’s main findings highlighted the urgent need for effective sediment management strategies in arid regions, where sedimentation rates are notably higher than in other regions. Full article
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22 pages, 10555 KB  
Article
Deep Learning-Based Recognition of Arch-Back Direction in Bare-Root Strawberry Seedlings for Mechanized Transplanting
by Jinhao Zhou, Pengcheng Zhang, Menglei Wei, Wei Liu, Jiawei Shi, Youheng Tan and Jianping Hu
Agriculture 2026, 16(6), 657; https://doi.org/10.3390/agriculture16060657 - 13 Mar 2026
Viewed by 64
Abstract
Correct arch-back orientation is essential in ridge-based strawberry transplanting. Improper orientation can increase soil contact and soil-borne disease risk, leading to yield loss and reduced harvest efficiency. In current practice, arch-back orientation of bare-root seedlings is still mainly judged and corrected manually, which [...] Read more.
Correct arch-back orientation is essential in ridge-based strawberry transplanting. Improper orientation can increase soil contact and soil-borne disease risk, leading to yield loss and reduced harvest efficiency. In current practice, arch-back orientation of bare-root seedlings is still mainly judged and corrected manually, which is labor-intensive and not always accurate under field conditions. Although plug seedlings are easier for mechanized transplanting, they are about three times more expensive than bare-root seedlings. Therefore, bare-root seedlings remain widely used for cost-effective production. However, accurate real-time orientation perception for bare-root seedlings is still challenging because stems are thin, morphology varies widely, and leaves often occlude key curvature cues. To address this gap, we propose a lightweight machine-vision method for bare-root strawberry seedlings that detects three characteristic keypoints on the new stem. The three-keypoint design is inspired by farmers’ practical judgement: farmers often determine arch-back direction by observing the stem and using manual touch to sense curvature changes. Similarly, three keypoints provide a simple geometric representation of curvature trend, enabling real-time estimation of both arch-back direction and bending angle. Physical tests on 100 bare-root seedlings achieved a 93% agronomically compliant orientation rate, with an MAE of 5.74° and an RMSE of 7.44° for bending-angle estimation. For edge deployment, the optimized model achieved real-time performance on an embedded GPU platform, reaching 152.51 FPS (FP16) and 154.26 FPS (INT8). Overall, the proposed method provides a practical perception module that can be integrated into strawberry transplanting machines to support cost-effective, orientation-aware mechanized transplanting. Full article
(This article belongs to the Section Agricultural Technology)
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25 pages, 3363 KB  
Article
Spatial Clustering of Front Yard Landscapes: Implications for Urban Soil Conservation and Green Infrastructure Sustainability in the Río Piedras Watershed
by L. Kidany Sellés and Elvia J. Meléndez-Ackerman
Sustainability 2026, 18(6), 2821; https://doi.org/10.3390/su18062821 - 13 Mar 2026
Viewed by 157
Abstract
Current sustainability discourse promotes sustainable yard practices as a means for residents to contribute to urban environmental health and soil conservation. Social–ecological research suggests that yard practices are shaped by multiscale social drivers, including social contagion, whereby visible expressions of individuality in front [...] Read more.
Current sustainability discourse promotes sustainable yard practices as a means for residents to contribute to urban environmental health and soil conservation. Social–ecological research suggests that yard practices are shaped by multiscale social drivers, including social contagion, whereby visible expressions of individuality in front yard design are copied by nearby neighbors. This study evaluated residential areas within the Río Piedras Watershed (RPWS) in the San Juan metropolitan area to assess evidence of social contagion in front yard configuration and vegetation structure, and to examine whether these variables were associated with socio-demographic and economic characteristics when spatial effects were considered. A total of 6858 front yards across six highly urbanized sites were analyzed using Google Earth Street View imagery. Housing lot sizes were quantified, and yards were classified into eight landscape configurations based on green and gray cover elements. Woody vegetation structures, including trees, shrubs, and palms, were also quantified to generate estimates of functional diversity and a front yard quality index. Significant differences in yard characteristics were observed among sites. Spatial analyses revealed significant clustering at distances of 65–80 m, particularly for front yard configuration, while clustering of woody vegetation density was weaker. Local clustering patterns and the distribution of outliers varied across sites. Spatial lag models indicated that lot area positively influenced yard configuration and quality, and the density and diversity of woody vegetation. While socio-economic variables were not significant predictors of yard quality, their effects cannot be discarded. Overall, results are consistent with social contagion processes but also highlight neighborhood design as a key driver of clustering, alongside widespread conversion of green to paved front yards, with implications for soil and green infrastructure loss as well as environmental and human health in the RPWS. Full article
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18 pages, 12292 KB  
Article
Modeling Spatial Patterns of Soil Erosion Based on Land Use Changes and Landscape Fragmentation in Arid Regions
by Griselda Vázquez-Quintero, Martín Martínez-Salvador, Jesús A. Prieto-Amparan, Pamela F. Mejía-Leyva, María Cecilia Valles-Aragón, Myrna C. Nevárez-Rodríguez, Emily García-Montiel and Alfredo Pinedo-Alvarez
Land 2026, 15(3), 458; https://doi.org/10.3390/land15030458 - 13 Mar 2026
Viewed by 85
Abstract
Soil erosion is a growing environmental problem in arid regions, where land-use changes and landscape fragmentation directly influence land degradation. This study estimated soil loss in the Tarabillas sub-basin, located in the Chihuahuan Desert, Mexico. To this end, the Universal Soil Loss Equation [...] Read more.
Soil erosion is a growing environmental problem in arid regions, where land-use changes and landscape fragmentation directly influence land degradation. This study estimated soil loss in the Tarabillas sub-basin, located in the Chihuahuan Desert, Mexico. To this end, the Universal Soil Loss Equation (USLE) was applied and integrated with Geographic Information System (GIS) tools. Landsat TM and OLI satellite imagery were classified through supervised techniques, achieving overall accuracies above 89%. The analysis was supported by comparing erosion patterns associated with land-use changes occurring during the 1990–2021 period, assessed through cross-tabulation matrices and landscape metrics. The results show that although the average erosion potential of the sub-basin remained constant at approximately 12.45 t ha−1 yr−1, erosion redistributed spatially, concentrating in areas where agriculture has replaced natural vegetation. Shrublands and grasslands continue to dominate the high erosion categories due to their wide spatial extent and high erodibility. These findings highlight that fragmented agricultural expansion constitutes the main driver of landscape transformation and soil vulnerability, emphasizing the importance of integrating remote sensing, GIS, and empirical models to support sustainable land management in arid regions. Full article
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23 pages, 8944 KB  
Article
Impact of Clogging on the Infiltration Performance of Porous Asphalt Mixtures Under a GIS–USLE-Based Multiscale Assessment of Peri-Urban Sediment Loads: A Case Study in Boyacá, Colombia
by Andres Silva-Balaguera, Julian Villate-Corredor, Jessica Betancourt-Gonzalez, Karen Fuquene-Saenz and Luis Ángel Sañudo-Fontaneda
Water 2026, 18(6), 669; https://doi.org/10.3390/w18060669 - 13 Mar 2026
Viewed by 160
Abstract
Clogging is the main mechanism that deteriorates the hydraulic functionality of permeable pavements, particularly in porous asphalt mixtures (PAM). This study evaluated the hydraulic impact of sediments from three peri-urban micro-watersheds in the Boyacá region of Colombia on the infiltration capacity of PAM. [...] Read more.
Clogging is the main mechanism that deteriorates the hydraulic functionality of permeable pavements, particularly in porous asphalt mixtures (PAM). This study evaluated the hydraulic impact of sediments from three peri-urban micro-watersheds in the Boyacá region of Colombia on the infiltration capacity of PAM. Road infrastructure and drainage conditions were analysed using orthophotos and field inspections to identify geomorphological factors that favour sediment transport toward the roadway. Annual erosion rates were estimated using the Universal Soil Loss Equation (USLE), and sediments were characterized both within the watersheds and at their outlet onto the road. Hydraulic performance was assessed through laboratory tests using a Falling Head Permeameter, complemented by field infiltration measurements with a Modified Cantabrian Infiltrometer (0.25 m2). Results showed erosion rates of up to 7.9 t/ha·year and infiltration losses above 90% under clogged conditions. A partial hydraulic recovery of around 40% was observed after maintenance, particularly when sediments exhibited a higher sand fraction. These findings demonstrate that combining USLE-based erosion modelling with controlled hydraulic testing provides a robust framework for evaluating clogging risks in peri-urban roads and offers new evidence on the hydraulic behaviour of PAM exposed to non-urban sediments in the design and maintenance of sustainable pavements. Full article
(This article belongs to the Special Issue Urban Drainage Systems and Stormwater Management, 2nd Edition)
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17 pages, 6126 KB  
Article
Sustainable Agricultural Practices for Managing Rice Crops to Minimize Environmental Contamination from the Pesticide Imazamox
by Antonio López-Piñeiro, Luis Vicente, Manuel Pérez, Damián Fernández-Rodríguez and David Peña
Agronomy 2026, 16(6), 609; https://doi.org/10.3390/agronomy16060609 - 12 Mar 2026
Viewed by 153
Abstract
Weed management is crucial for the sustainable production of rice (Oryza sativa L.), although herbicides such as Imazamox (IZX) can persist in soils, posing risks to soils and water resources. This two-year study evaluated the effects of soil physicochemical properties under different [...] Read more.
Weed management is crucial for the sustainable production of rice (Oryza sativa L.), although herbicides such as Imazamox (IZX) can persist in soils, posing risks to soils and water resources. This two-year study evaluated the effects of soil physicochemical properties under different irrigation and tillage practices, with and without compost derived from olive mill waste, on IZX behavior. The treatments implemented were as follows: no-tillage and sprinkler (NT-S), conventional tillage and sprinkler (T-S), conventional tillage and flooding (T-F), and the corresponding regimes with compost amendment (NT-SC, T-SC, and T-FC). Sorption–desorption, dissipation, and leaching of the herbicide were assessed. The IZX adsorption was lower under soil collected from sprinkler irrigation, especially in NT-S, while compost reduced the adsorption under T-SC and T-FC. Dissipation was faster in NT-S and T-S soils, in which the half-life of IZX declined up to 30% relative to T-F. Furthermore, compost further accelerated herbicide dissipation, correlating with higher organic carbon content and microbial activity. The IZX losses via leaching were significantly reduced in soils irrigated by sprinkler in combination with compost, with values ≤ 48.5% of the IZX applied. These results indicate that the irrigation regime and organic amendment strongly influence soil physicochemical properties, then influencing the environmental fate of IZX. Integrated management using sprinkler irrigation and compost can mitigate IZX persistence and leaching, improve soil health, and reduce the risk of water contamination, representing a sustainable strategy for rice cultivation. Full article
(This article belongs to the Special Issue Soil Health and Properties in a Changing Environment—2nd Edition)
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23 pages, 7688 KB  
Article
Mechanisms of Fouled Railway Ballast Deterioration Under Freeze–Thaw and Cyclic Loading: Implications for Sustainable Maintenance in Seasonal Frozen Regions
by Dongjie Zhang, Qionglin Li, Shanhao Li, Kai Cui, Xiaotong Qin, Zhanyuan Zhu and Zhijia Zhang
Sustainability 2026, 18(6), 2808; https://doi.org/10.3390/su18062808 - 12 Mar 2026
Viewed by 111
Abstract
Maintaining ballast performance in seasonal frozen regions is essential for resilient and sustainable railway infrastructure because freeze–thaw-driven fouling can shorten service life and increase maintenance-related material consumption. To investigate the deterioration mechanisms of fouled railway ballast in seasonal frozen regions, freeze–thaw cycle tests [...] Read more.
Maintaining ballast performance in seasonal frozen regions is essential for resilient and sustainable railway infrastructure because freeze–thaw-driven fouling can shorten service life and increase maintenance-related material consumption. To investigate the deterioration mechanisms of fouled railway ballast in seasonal frozen regions, freeze–thaw cycle tests and cyclic loading model tests were conducted in sequence using a custom low-temperature geotechnical system. The test results processed by Origin software indicate that unfrozen water migrates toward the freezing front under temperature gradients and forms ice lenses during freezing. During thawing, meltwater is retained above the underlying frozen soil. Repeated freeze–thaw cycles therefore promote progressive water accumulation in the upper soil layers, eventually forming a clay layer with high water content. Under cyclic loading, interlayer thickening exhibited clear moisture thresholds relative to the clay liquid limit (LL = 24%). Below the LL (18–24%), ballast penetration and fines migration were limited and thickness increased slowly. Above the LL, rapid strength loss accelerated penetration and upward transport. At an initial water content of 32%, fines migration surpassed the ballast surface and the ballast became fully fouled, meaning that the fouled interlayer thickness equaled the full 100 mm ballast-layer thickness. Fouling severity increased sharply with moisture: the void contaminant index exceeded the maintenance criterion (VCI > 40%) at 28% water content and evolved into severe mud pumping at higher concentrations. Excess pore water pressure developed stratification with depth, maintaining an upward hydraulic gradient near the interface and yielding a net water loss of 2.24–6.91% in the upper fine-grained layer. These quantified thresholds and mechanistic insights provide actionable trigger points for condition-based maintenance and climate-adaptive design, helping extend track-bed service life and reduce resource-intensive ballast renewal in seasonal frozen regions. Full article
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16 pages, 4782 KB  
Article
Phenotypic and Genomic Characterization of Polyethylene-Degrading Bacillus cereus PE-1 Enriched from Landfill Microbial Consortium
by Weijun Wang, Shunyu Yao, Zhimin Liu and Xiaolu Liu
Polymers 2026, 18(6), 695; https://doi.org/10.3390/polym18060695 - 12 Mar 2026
Viewed by 132
Abstract
Polyethylene (PE) is one of the most persistent pollutants in the environment. Here, we enriched a microbial consortium (PEH) and isolated a bacterial strain, Bacillus cereus PE-1, capable of degrading PE from landfill soil using PE as the sole carbon source. Scanning electron [...] Read more.
Polyethylene (PE) is one of the most persistent pollutants in the environment. Here, we enriched a microbial consortium (PEH) and isolated a bacterial strain, Bacillus cereus PE-1, capable of degrading PE from landfill soil using PE as the sole carbon source. Scanning electron microscopy revealed significant surface erosion, while weight loss reached up to 4.57% after 30 days. TGA showed a 5.88% decrease in onset degradation temperature, and contact angle measurements indicated increased hydrophilicity. Elemental analysis confirmed oxygen incorporation into the polymer matrix. Genome sequencing revealed genes associated with biofilm formation (epsA, epsB, pgaC), oxidation (laccase, copper oxidase), hydrolysis (esterase, lipase, PHB depolymerase), and β-oxidation pathways. While these genomic findings indicate a predicted capacity for assimilation, no transcriptomic or proteomic validation was performed in this study. These findings suggest that PE-1 can colonize PE, initiate oxidative cleavage, and potentially assimilate breakdown products. This study provides new insights into the microbial degradation of polyolefins and identifies a promising bacterial candidate for plastic bioremediation. Full article
(This article belongs to the Special Issue Application and Degradation of Polymeric Materials in Agriculture)
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23 pages, 5616 KB  
Article
Informer–UNet: A Hybrid Deep Learning Framework for Multi-Point Soil Moisture Prediction and Precision Irrigation in Winter Wheat
by Dingkun Zheng, Chenghan Yang, Gang Zheng, Baurzhan Belgibaev, Madina Mansurova, Sholpan Jomartova and Baidong Zhao
Agriculture 2026, 16(6), 648; https://doi.org/10.3390/agriculture16060648 - 12 Mar 2026
Viewed by 136
Abstract
Soil moisture prediction is essential for precision irrigation in water-limited agricultural systems. This study presents a deep learning-driven irrigation framework for winter wheat, integrating a novel Informer–UNet model with a Comprehensive Irrigation Index for adaptive water management. The Informer–UNet combines ProbSparse self-attention mechanisms [...] Read more.
Soil moisture prediction is essential for precision irrigation in water-limited agricultural systems. This study presents a deep learning-driven irrigation framework for winter wheat, integrating a novel Informer–UNet model with a Comprehensive Irrigation Index for adaptive water management. The Informer–UNet combines ProbSparse self-attention mechanisms with UNet’s multi-scale feature fusion, enabling simultaneous prediction of soil moisture at 27 monitoring points across three depths, 10, 30, and 50 cm, while quantifying prediction uncertainty through Monte Carlo Dropout. A Comprehensive Irrigation Index incorporating moisture deviation, spatial variance, and confidence interval width was developed, with weights optimized via genetic algorithm. Field experiments were conducted in Chengdu, China, over two winter wheat growing seasons. The Informer–UNet achieved superior prediction accuracy, R2 greater than 0.98, RMSE less than 0.65, compared to LSTM, Transformer, and standard Informer models, with the fastest convergence and lowest validation loss. The proposed DeepIndexIrr strategy maintained soil moisture within the target range, 55% to 75%, for over 81% of the irrigation period, reducing water consumption by 38.2% compared to fixed-threshold control and 19.2% compared to expert manual scheduling. These results demonstrate that integrating spatially distributed deep learning predictions with uncertainty-informed decision rules offers a promising approach for sustainable precision irrigation. Full article
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17 pages, 4932 KB  
Article
Urbanization-Induced Shifts in Microbial Functional Genes of Wetland Nitrogen Cycling Promote Nitrous Oxide (N2O) Emissions
by Xinyu Yi, Yuwen Lin, Yinghe Peng, Yan Liu, Chen Ning, Junjie Lei, Ling Wang, Chan Chen, Linshi Wu and Juyang Liao
Microorganisms 2026, 14(3), 640; https://doi.org/10.3390/microorganisms14030640 - 12 Mar 2026
Viewed by 151
Abstract
Urban wetlands are assumed to contribute to nitrous oxide (N2O) emissions; however, the microbial mechanisms underlying enhanced N2O fluxes in urban wetlands and differences in microbial responses between aquatic and soil compartments have not been clearly identified. Here, we [...] Read more.
Urban wetlands are assumed to contribute to nitrous oxide (N2O) emissions; however, the microbial mechanisms underlying enhanced N2O fluxes in urban wetlands and differences in microbial responses between aquatic and soil compartments have not been clearly identified. Here, we characterized the nitrogen (N) cycling microbial communities and their functional metabolic pathways in urban and rural wetlands using metagenomics and N2O flux measurements. Results showed that urbanization drove a 6~8-fold increase in N2O fluxes from urban wetlands compared to rural wetlands. Structural equation modeling (SEM) confirmed that urbanization intensity was a primary driver (standardized coefficients: 0.72 for soil and 0.92 for water). In wetland water, N2O emissions were negatively correlated with inorganic nutrient concentrations (coefficient = −0.62). Aquatic microbial communities exhibited substantial taxonomic shifts but preserved network connectivity, indicating adaptive strategies for surviving urban perturbations at the cost of reduced functional redundancy. In wetland soil, microbial communities maintained stability under urbanization, which was attributed to environmental buffering from heterogeneous microenvironments. Soil N2O emissions were positively linked to microbial alpha diversity (coefficient = 0.79). Furthermore, urban wetlands enriched genes mediating nitrification and denitrification while depleting genes associated with N fixation and organic N metabolism. This functional shift reflects microbial specialization in processing elevated reactive N (Nr) inputs from urban sources, trapping urban wetlands in an “N loss loop” that reinforces high N2O fluxes. This study elucidates the microbial mechanisms governing wetland N2O emissions under urbanization, thereby enhancing understanding of microbially mediated N cycling in the urban wetland ecosystem. Full article
(This article belongs to the Section Environmental Microbiology)
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18 pages, 3831 KB  
Article
Combined Controlled-Release and Common Fertilizer Application Increases Apple Productivity by Optimizing Soil Nutrient and Microbial Communities
by Junyin Li, Shan Li, Denglun Chen, Zekun Wang, Wanting Qi, Pengxiao Ren, Xiaoqian Pei, Shugang Zhang and Yuechao Yang
Horticulturae 2026, 12(3), 339; https://doi.org/10.3390/horticulturae12030339 - 11 Mar 2026
Viewed by 82
Abstract
Apples in China are planted mainly in nutrient-poor mountain soil, and a large amount of fertilizer input results in resource waste and a decrease in nutrient utilization efficiency. Controlled-release fertilizer (CRF) has been shown to be environmentally friendly and increase crop yield, but [...] Read more.
Apples in China are planted mainly in nutrient-poor mountain soil, and a large amount of fertilizer input results in resource waste and a decrease in nutrient utilization efficiency. Controlled-release fertilizer (CRF) has been shown to be environmentally friendly and increase crop yield, but nutrient release cannot be precisely synchronized with apple demand. Here, a suitable secondary fertilization method was established by a two-year apple field experiment with CRF and common compound fertilizer (CF) at various ratios under a 25% reduction in application. The application of CF and CRF changes the temporal and spatial distributions of soil NPK nutrients, decreasing NPK losses and NH3 emissions. The NH3 emissions under CF and CRF decreased by 17.98–44.86%, as N loss decreased by 11.59–29.81% and by 4.45–8.19%, with respect to those under CF alone, while the soil pH and electrical conductivity increased by 8.28–17.12% and 10.73–18.29%, compared with those under CF alone. The increase in soil P and K also decreased losses by 8.28–17.12% and 10.73–18.29%. The combined application of CF and CRF can increase soil microbial diversity and functional taxa and nutrient cycling genes, resulting in efficient nutrient transformation and supply for apple trees. The regulation of nutrients and microbes by the secondary application of CF and CRF drives an increase in apple yield of 23.71–54.32%, resulting in high economic benefits. In total, the application ratio of CF and CRF at 3:7 in March and July was an effective way to balance apple productivity and the soil ecological environment, providing a sustainable solution for mountainous orchard ecosystems globally. Full article
(This article belongs to the Special Issue Sustainable Soil Fertility and Nutrient Management in Horticulture)
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