Search Results (5,800)

Introduced species of trees and shrubs used in landscaping of cities in the steppe zone are exposed to the combined negative impact of the ever-increasing load of various anthropogenic factors and unfavorable zonal natural and climatic conditions. In this regard, the assessment of the degree of plant resistance to unfavorable factors in the urban ecosystems of the steppe zone is a necessary condition for rationalizing the selection of the assortment and improving the condition of green spaces. This paper presents the results of the analysis of the vital state of 5509 representatives of 78 introduced species of trees and shrubs growing along the road and transport network in the territory with increased anthropogenic pressure. The age structure of plantings, as well as a number of biological and ecological characteristics of the species composition, are analyzed. The variation in the level of vitality in groups united by individual characteristics—taxonomic affiliation, geographical origin, morphobiological characteristics (habitus), growth rate and age of plants—is shown, and groups with the highest level of vitality are identified. As a result, a number of criteria are selected that can serve as indirect markers of plant adaptability to the ecological conditions of steppe zone cities when forming an assortment for landscaping. Using the examples of the features “plant height” and “plant age”, the species-specific reaction of plants is shown, expressed in the limitation of growth and development, as well as the reduction of life expectancy under conditions of increased anthropogenic and climatic loads. The data obtained can be used to adjust the species composition of urban trees and shrubs, optimize their ratio and spatial and functional placement, and thereby optimize the operational characteristics of green spaces and increase the duration of their use. Full article

The chemical profile of coffee depends on numerous factors, the complexity of which makes it difficult to clearly assess their influence. The aim of this study was to comprehensively evaluate the impact of selected coffee brewing methods (Espresso, Simple Infusion, French Press, V60), taking into account the coffee species (Arabica, Robusta, Blends), the degree of roasting (light, medium, dark) and the geographical origin (single-origin and multi-origin) on the chemical composition of the brew. Eighteen different types of coffee, which differ in the aforementioned characteristics, were analyzed. The caffeine content (using high-performance liquid chromatography), the total phenolic content (TPC; using a spectrophotometric method), and selected minerals (calcium, iron, potassium, magnesium, sodium, phosphorus, zinc; using Inductively Coupled Plasma–Optical Emission Spectrometry) were analyzed. The analysis showed that both the brewing method and the species had a significant influence on the chemical profile of the resulting brews, while the degree of roasting and the origin showed no significant influence. The Espresso method showed the highest caffeine, TPC, potassium, magnesium, and phosphorus content, the V60 method—calcium, iron, and sodium, and the French Press and Simple Infusion methods showed intermediate values. Robusta coffee contained more caffeine and TPC, Arabica contained more magnesium, and Blend showed medium values for both species. The results obtained may have practical implications for both consumers and the coffee industry, supporting informed decision-making and the refinement of brewing methods. Full article

Hygrophila polysperma is a type of amphibious plant that originates from Acanthaceae. Here, we report its first complete chloroplast (cp) genome. The complete cp genome is 146,675 bp in length with 38.3% of GC content. There are 130 genes including 86 protein coding genes, 36 tRNA genes, and 8 rRNA genes in this genome. Simple short sequence (SSR) analysis found 30 SSRs, 24 of which are located in a large single-copy region. Nucleotide diversity identified six most divergent sequences (trns-GCU, psaA-pafI, psaI-pafII, ycf2, rpl32, and ycf1) among 3 close-related species, H. polysperma, H. ringens, and Asteracantha longifolia. A phylogenetic tree among H. polysperma and another 30 related species was constructed based on the common coding sequence of the cp genome and showed that H. polysperma is most closely related to H. ringens (both belong to subtribe Hygrophilinae) and, together, they form a clade that is sister to A. longifolia. This study provides a basis for systemic and evolution studies as well as the development of molecular markers for species identification and genetic breeding. Full article

Phoebe bournei is an important economic tree species in China, its large-scale propagation is limited by the difficulty of adventitious root (AR) formation in cuttings. In this study, morphological, physiological, and transcriptomic analyses were conducted to investigate the process of AR formation in P. bournei. The results showed that ARs mainly originated from callus tissue. During AR formation, soluble sugar and soluble protein contents changed significantly. Malondialdehyde (MDA) and oxygen free radicals (OFRs) peaked at first sampling stage (PB0), while the activities of polyphenol oxidase (PPO) and indoleacetic acid oxidase (IAAO) exhibited similar patterns. Lignin content increased during callus induction stage, whereas phenolic content continuously declined throughout rooting. Endogenous hormone levels also changed markedly, and Orthogonal partial least squares discriminant analysis (OPLS-DA) analysis indicated that indole-3-acetic acid (IAA) and abscisic acid (ABA) played dominant roles in this process. KEGG enrichment analysis revealed significant enrichment of the phenylpropanoid biosynthesis pathway in all three comparison groups. A total of 48 differentially expressed genes (DEGs) were enriched in plant hormone signal transduction pathways, with 22 and 14 genes associated with IAA and ABA signaling, respectively. Weighted gene co-expression network analysis (WGCNA) further identified two hub modules related to IAA and ABA contents, including eight hub genes such as D6PKL1 and ISTL1. Correlation analysis revealed that the hub genes D6PKL1 and HSP were significantly positively correlated with IAA4 in the IAA signaling pathway. Overall, this study provides new insights into the mechanisms underlying AR formation in P. bournei cuttings and offers a theoretical basis for optimizing its clonal propagation system. Full article

Cancer-associated fibroblasts (CAFs) are key components of the tumor microenvironment (TME) that influence cancer progression via extracellular matrix (ECM) remodeling and secretion of growth factors and cytokines. Endothelial-to-mesenchymal transition (EndMT) is emerging as an important axis among the heterogeneous origins of CAFs. This review introduces the diverse methods used to induce EndMT in cancer—mouse tumor models, conditioned-medium treatment, co-culture, targeted gene perturbation, ligand stimulation, exosome exposure, irradiation, viral infection, and three-dimensional (3D) culture systems—and summarizes EndMT cell-type evidence uncovered using transcriptomic and proteomic technologies. Hallmark EndMT features include spindle-like morphology, increased motility, impaired angiogenesis and barrier function, decreased endothelial markers (CD31, VE-cadherin), and increased mesenchymal markers (α-SMA, FN1). Reported mechanisms include signaling via TGF-β, cytoskeletal/mechanical stress, reactive oxygen species, osteopontin, PAI-1, IL-1β, GSK-3β, HSP90α, Tie1, TNF-α, HSBP1, and NOTCH. Cancer-induced EndMT affects tumors and surrounding TME—promoting tumor growth and metastasis, expanding cancer stem cell-like cells, driving macrophage differentiation, and redistributing pericytes—and is closely associated with poor survival and therapy resistance. Finally, we indicate each study’s stance: some frame cancer-induced EndMT as a source of CAFs, whereas others, from an endothelial perspective, emphasize barrier weakening and promotion of metastasis. Full article

To investigate the geographical and species differences regarding mineral element content of camel milk, this research used camel milk from the Tacheng, Altay, and Ili regions of Xinjiang and cow milk, goat milk, and horse milk from the Tacheng region as subjects. The contents of 22 mineral elements were measured using inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometry (ICP-OES). The results showed that the contents of macro elements Ca, P, K, and Na in camel milk were significantly higher than those in other milk sources (p < 0.01). The contents of trace elements such as Se, Sr, and Ni were very significantly higher than those in other milk sources (p < 0.01). The content of 12 mineral elements in camel milk was very significantly higher than in other types of milk (p < 0.01). Principal component analysis (PCA) and factor analysis emphasized the relationship between element distribution and different milk sources, and the linear discriminant analysis (LDA) model could identify the species type of milk. Geographical analysis indicated that trace elements such as Sr, Ni, and Cr were highly significantly enriched in Tacheng camel milk (p < 0.01). The established LDA model achieved traceability of the geographical origin of Xinjiang camel milk. This research reveals the mineral nutritional advantages of camel milk and its geographical differentiation patterns, providing theoretical support for exploring the functional properties of camel milk and for identifying species and regions through minerals. It is important to promote the upgrading of the specialty dairy product industry. Full article

Polycyclic aromatic hydrocarbons (PAHs) are persistent organic pollutants with significant ecological and public health implications, particularly in urban wetlands exposed to chronic anthropogenic stress. This study evaluates the occurrence, spatial distribution, seasonal variability, and ecological risk of three low molecular weight PAHs—naphthalene (NAP), phenanthrene (PHEN), and anthracene (ANT)—in surface waters of Caño La Malaria, the main freshwater source of Cucharillas Marsh, Puerto Rico’s largest urban wetland. Surface water samples were collected at four locations during both wet- and dry-season campaigns. Samples were extracted and quantified by GC-MS. NAP was the dominant compound, Σ3PAHs concentrations ranging from 7.4 to 2198.8 ng/L, with higher wet-season levels (mean = 745.79 ng/L) than dry-season levels (mean = 186.71 ng/L); most wet-season samples fell within the mild-to-moderate contamination category. Compositional shifts indicated increased levels of PHEN and ANT during the wet season. No significant spatial differences were found (p = 0.753), and high correlations between sites (r = 0.96) suggest uniform input sources. Diagnostic ratios, inter-species correlations, and principal component analysis (PCA) consistently indicated a predominant pyrogenic origin, with robust PHEN–ANT correlation (r = 0.824) confirming shared combustion-related sources. PCA revealed a clear separation between dry- and wet-season samples, with the latter showing greater variability and stronger associations with NAP and ANT. Ecological risk assessment using hazard quotients (HQ_water) indicated negligible acute toxicity risk across all sites and seasons (<0.01); the highest HQ_water (0.0095), observed upstream during the wet season, remained within this range. However, benchmark exceedances by PHEN and ANT suggest potential chronic risks not captured by the acute ERA framework. These findings support integrated watershed management practices to mitigate PAH pollution and strengthen long-term ecological health in tropical urban wetlands. Full article

Seafood plays a key role in a healthy diet due to its high content of essential nutrients. However, its global trade and complex supply chains expose it to frequent mislabeling and food fraud. This study investigates Italian consumers’ willingness to pay (WTP) for traceable seafood products, exploring how blockchain technology (BT) can enhance transparency and economic sustainability in the fish supply chain. An online questionnaire, administered in 2022 and 2024, gathered responses from a diverse demographic, focusing on four representative seafood species: farmed sea bass, striped venus clams, giant red shrimp, and albacore tuna. Results indicate that most respondents—primarily with higher education levels—value traceability and are willing to pay a premium for certified, traceable seafood. The study models the economic feasibility of implementing BT at both national and regional levels and finds that the consumer’s WTP exceeds the additional costs incurred by adopting BT. These findings support the viability of traceability systems in improving food safety and sustainability, while reinforcing consumer trust. The results also underscore the importance of providing clear information at the point of purchase, particularly regarding species, origin, and production methods—factors critical to informed seafood choices and advancing more sustainable consumer behavior in Italy. Full article

Salvia fruticosa, S. officinalis, S. pomifera ssp. pomifera, S. ringens, and S. tomentosa have multiple potential uses in floriculture and the pharmaceutical industry, serving sustainable horticulture and landscaping. The aim was to develop effective asexual propagation protocols for the exploitation of the above species. Thus, the effect of cutting origin, season of cutting collection, and various indole-3-butyric acid (IBA) treatments on rooting stem cuttings was examined. Shoot-tip cuttings were collected either from greenhouse or wild mother plants, in November, February, May, and August and were treated either with Rhizopon dusting powder 0.5% w/w IBA or immersion for 1 min in 0–6000 mg L⁻¹ IBA solution. The cuttings were then placed for rooting in a 1:1 (v/v) peat–perlite substrate, under mist, for 2 weeks and on the greenhouse bench in semi-shade for another 4 weeks. More efficient rooting was succeeded by cuttings, (i) of S. tomentosa, followed by S. fruticosa and S. pomifera ssp. pomifera, while S. officinalis was the most difficult to root, (ii) from greenhouse plants, (iii) collected in autumn or spring, and (iv) treated with Rhizopon dusting powder or 1500 mg L⁻¹ IBA solution. Higher dry weight values of the rooted cuttings were found in autumn. Conclusively, rooting of Salvia spp. cuttings depended on species, mother plants’ physiological state, time of cutting collection, climatic conditions, and auxin application. Full article

Australian plants of the family Haemodoraceae have been a reliable source of new secondary metabolites, particularly those of the ‘phenylphenalenone’ class, and related chromenes and xanthones. Some of these compounds demonstrate anti-microbial properties against both Gram-negative and Gram-positive bacteria. Chemical profiling of thirty individual ethanolic extracts from six separate species of Australian plants belonging to the family Haemodoraceae was conducted using an HPLC-MS approach reinforced by HRLC(ESI)-MS. Six of the extracts were further explored by employing HRLC(ESI)-MS and the compounds present were characterised and confirmed based on a comparison to the original data. All thirty extracts were assessed for biological activity against the parasitic nematode Haemonchus contortus in vitro. The chemical profiling methodology adopted resulted in the identification of thirty-four previously reported compounds, identifying on average 64% of the previously reported secondary metabolites across the species Haemodorum simulans, Haemodorum spicatum, Haemodorum brevisepalum and Macropidia fuliginosa. Furthermore, compounds from the phenylbenzoisoquinolindone class were detected in the bulbs of Haemodorum simulans and Haemodorum coccineum, representing the first report of the structure class in extracts of the genus Haemodorum. Extracts of the H. simulans stems, M. fuliginosa bulbs and H. distichophyllum roots and bulbs exhibited anthelmintic activity in vitro. The chemical profiling HPLC-MS methodology adopted was successful in the rapid identification of most of the previously reported secondary metabolites across the Haemodoracae species, indicating that the analytical approach was robust. This study demonstrates the effectiveness of dereplication via HPLC-MS-based chemical profiling across six Australian Haemodoraceae species, identifying numerous known and putatively novel secondary metabolites. It also reports, for the first time, anthelmintic activity in selected species and marks the first detailed phytochemical investigation of H. distichophyllum since its initial pigment analysis over 50 years ago. Full article

The increased presence of harmful algal blooms (HABs) is a concern for many aquatic environments, especially with the increasing effects of climate change. Members of the dinoflagellate genus Dinophysis have been shown to produce toxins that can cause Diarrheic Shellfish Poisoning (DSP) in humans who consume infected shellfish. The advancing oyster aquaculture industry in Delaware will require the development of management practices and monitoring HAB species to protect environmental and human health. Temperature, nutrients, and prey abundance can be drivers of Dinophysis blooms. D. acuminata has been historically identified at high concentrations (>200,000 cells L⁻¹) in water samples from Rehoboth Bay, DE, USA. However, the reach of spring blooms and how far they extend to aquaculture sites have not been determined. This study monitored an emergent HABs threat of a toxin-producing dinoflagellate, Dinophysis acuminata, by assessing a transect during the first recorded winter bloom in Torquay Canal and analyzing concentrations of chemical nutrients of combined nitrate and nitrite, and orthophosphate. Pearson correlation coefficient analysis between cell density (cells L⁻¹) and environmental variables across all sites was conducted to determine significant relationships between water temperature, Chl-a concentration, conductivity, dissolved oxygen (DO), combined nitrate and nitrite concentrations (NO_x), and orthophosphate concentrations (PO₄³⁻). Genetic techniques and PCR were utilized to determine the presence of Dinophysis using genus-specific primers to monitor cell density or abundance within the sediments during winter months. There were no significant correlations between environmental variables, and nutrient concentrations did not exceed EPA regulations. Molecular analyses of benthic sediments detected Dinophysis spp., offering insight into potential bloom origins. Overall, there is limited ecological data on Dinophysis acuminata in Rehoboth Bay, DE, USA. The results of this study will help strengthen resources for monitoring HAB species and understanding potential risks to oyster aquaculture in Delaware. Full article

Microplastics are considered to be one of the major threats that have significant effects on marine ecosystems and marine organisms. These tiny plastic particles can also absorb and carry toxic substances to marine life, potentially affecting human health through food chains. This study investigates microplastics in surface seawater and in two species of sea cucumber, Holothuria atra at Similan Island and Holothuria edulis at Surin Island. Color, shape, and components of microplastics were identified to evaluate sources of the microplastics found in the area. The results found that the average abundance of microplastics in seawater at Similan and Surin Islands is 1.93 ± 1.42 and 1.11 ± 0.75 pieces/m³, respectively. Black fiber is a dominant microplastic found in seawater and both species of sea cucumber. Fourier-Transform Infrared spectroscopy (FTIR) indicated that polyethylene terephthalate (PET) and polyester are the major components of microplastics in surface seawater, while cotton blends and other mixed components are the major components in sea cucumbers. These findings imply that microplastics found in surface seawater could potentially degrade from fishing and aquaculture equipment, maritime transport, and materials from plastic containers. Microplastics in sea cucumbers, on the other hand, are probably originating from domestic sewage discharge, especially textile washing and fishing equipment. Full article

The woolly whitefly, Aleurothrixus floccosus, is likely a Neotropical origin species that has spread globally. Introduced to France in 1969, it became a pest in southern European citrus groves, first reported in Italy in 1974. Integrated management using biological control agents is crucial due to the low efficacy of chemical controls. Nymphs produce waxy filaments and honeydew, limiting insecticide contact. Natural enemies, especially from Neotropics, have been documented. The parasitoids Amitus spiniferus and Cales noacki were released in France in 1970 and later observed in Liguria, Italy. In the Campania region, C. noacki was first found on Aleurotuba jelineki in 1984 and this finding preceded the first report of A. floccosus in the same area. Subsequently, C. noacki was also introduced in other regions where it showed better adaptation throughout the Italian territory, reaching high parasitization levels on the woolly whitefly nymphs. After many years since the last field investigations, surveys in 2024–2025 in organic citrus groves in central and southern Italy identified additional parasitoids. Besides C. noacki and A. spiniferus, Eretmocerus paulistus and Signiphora xanthographa were found for the first time in Italy. Both species were originally described from the Neotropical ecozone. The aphelinid finding represents its first documented establishment in Italy, while the signiphorid one represents a new record for the European fauna. E. paulistus is a primary parasitoid, while S. xanthographa is a hyperparasitoid that can limit the effectiveness of other parasitoids. The interaction of these parasitoids resulted in high parasitism rates for A. floccosus nymphs. Preserving the current complexity of parasitoids in integrated pest management (IPM) programs could effectively control the woolly whitefly in central and southern Italy. Full article

The aim of this study was to present a comprehensive analysis of honey varieties from different botanical origins, focusing on their phenolic compounds’ composition, volatile profiles, and antioxidant activity. We simultaneously identified and quantified 37 bioactive compounds, including anthocyanins, flavonols, flavones, flavan-3-ols, proanthocyanidins, and phenolic acids, across various honey samples by HPLC-MS/MS. Total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity (AOA) were determined using UV-Vis spectrophotometric analysis. The content of phenolic compounds quantified by HPLC-MS/MS ranged from 19.56 to 243.94 mg·kg⁻¹, highlighting a high presence of these antioxidant compounds (mainly phenolic acids), confirmed also by the positive correlation between TPC and DPPH values. Among volatiles compounds, analyzed by HS-SPME-GC-MS, benzene acetaldehyde and furfural resulted specific for two types of honey samples (H-7 and H-9), highlighting the possibility of searching for chemical markers to characterize honeys of different specie/origin. This study enhances our understanding of the bioactive potential of honey from different botanical origins and provides a foundation for future research on its health benefits. Full article

Jets and outflows are key components of low-mass star formation, regulating accretion and shaping the surrounding molecular clouds. These flows, traced by molecular species at (sub)millimeter wavelengths (e.g., CO, SiO, SO, H₂CO, and CH₃OH) and by atomic, ionized, and molecular lines in the infrared (e.g., H₂, [Fe II], [S I]), originate from protostellar accretion disks deeply embedded within dusty envelopes. Jets play a crucial role in removing angular momentum from the disk, thereby enabling continued mass accretion, while directly preserving a record of the protostar’s outflow history and potentially providing indirect insights into its accretion history. Recent advances in high-resolution, high-sensitivity observations, particularly with the James Webb Space Telescope (JWST) in the infrared and the Atacama Large Millimeter/submillimeter Array (ALMA) at (sub)millimeter wavelengths, have revolutionized studies of protostellar jets and outflows. These instruments provide complementary views of warm, shock-excited gas and cold molecular component of the jet–outflow system. In this review, we discuss the current status of observational studies that reveal detailed structures, kinematics, and chemical compositions of protostellar jets and outflows. Recent analyses of mass-loss rates, velocities, rotation, molecular abundances, and magnetic fields provide critical insights into jet launching mechanisms, disk evolution, and the potential formation of binary systems and planets. The synergy of JWST’s infrared sensitivity and ALMA’s high-resolution imaging is advancing our understanding of jets and outflows. Future large-scale, high-resolution surveys with these facilities are expected to drive major breakthroughs in outflow research. Full article