Search Results (17,326)

Carminic acid (CA) is a high-value natural anthraquinone pigment used in foods, cosmetics, textiles, and pharmaceuticals, but its current industrial supply depends largely on extraction from the scale insect Dactylopius coccus, creating constraints in yield, cost, sustainability, and allergen control. This review summarizes recent progress from pathway elucidation to microbial production. We first outline the structure, occurrence, applications, and biosynthetic logic of CA, emphasizing the convergence of type III polyketide assembly with insect-associated tailoring reactions, especially C-glycosylation. We then compare heterologous production strategies in Escherichia coli, Saccharomyces cerevisiae, Yarrowia lipolytica, and Aspergillus nidulans, focusing on chassis-specific advantages, bottlenecks, precursor supply, malonyl-CoA engineering, dynamic regulation, enzyme compatibility, compartmentalization, and downstream processing. Structurally related anthraquinone pigments are further discussed to extract broader design principles for pathway diversification and synthetic biology. Finally, we highlight key challenges for industrial translation, including low titers, incomplete enzyme characterization, host–pathway incompatibility, and scalable purification, and propose integrated strategies combining precursor-pathway rewiring, AI-assisted enzyme engineering, biosensor-based regulation, and process optimization to develop competitive microbial cell factories. Full article

Background/Objectives: The transition toward healthier and more sustainable diets has increased the need for reformulated bakery and confectionery products formulated with alternative ingredients. However, validated instruments for assessing consumer acceptance of these products remain limited. This study aimed to develop and psychometrically validate a multidimensional assessment framework integrating food choice motives, green (environmental) values, health consciousness, perceived nutritional value, willingness to pay a premium, and purchase intention. Methods: A cross-sectional survey was conducted among 504 consumers in Romania using an online questionnaire. The instrument was developed by adapting established scales and by developing a new Perceived Nutritional Value scale. Dimensionality was evaluated using exploratory (EFA) and confirmatory (CFA) factor analyses. Temporal stability was assessed in an independent test–retest subsample (n = 30; 14-day interval), and the proposed conceptual model was evaluated using Partial Least Squares Structural Equation Modeling (PLS-SEM) with 5000 bootstrap resamples. Robustness was examined through a sensitivity analysis excluding respondents younger than 18 years (N = 441). Results: EFA (KMO = 0.933) and CFA (CFI = 0.965, TLI = 0.959, RMSEA = 0.061, SRMR = 0.033) supported the proposed six-factor structure. All constructs demonstrated excellent internal consistency (Cronbach’s α: 0.898–0.938), convergent validity (AVE: 0.790–0.889), discriminant validity (HTMT < 0.77), and good-to-excellent test–retest reliability (ICC: 0.82–0.91). Perceived nutritional value was positively and significantly associated with both willingness to pay and purchase intention, and willingness to pay partially mediated the association between perceived nutritional value and purchase intention. The structural model explained 46.1% of the variance in purchase intention; all hypothesized relationships were supported, and the findings remained stable in the sensitivity analysis. Conclusions: The proposed framework demonstrated good psychometric performance and provides a theory-informed measurement framework for assessing consumer acceptance of reformulated bakery and confectionery products. It may support future nutrition research, consumer studies, and the evaluation of food reformulation strategies aimed at promoting healthier and more sustainable dietary choices. Full article

Background/Objectives: Food allergy prevention is no longer understood as the simple avoidance of allergenic foods. The first 1000 days (from conception to, approximately, 24 months of age) include prenatal, perinatal and postnatal windows in which diet, epithelial barrier integrity, microbial ecology, immune maturation and family-level implementation interact. This narrative review was developed as a Commission-proposed SIAIP-oriented clinical framework, rather than a formal guideline or consensus statement. Evidence from guidelines, systematic reviews, randomised trials, birth cohorts and mechanistic studies was organised by developmental window and interpreted according to endpoint validity, confounding, feasibility and clinical readiness. Prenatal factors include maternal allergen non-avoidance, whole-diet quality, micronutrient and fatty acid status, antibiotic exposure, environmental context and maternal microbiome. Perinatal factors include mode of delivery, intrapartum and neonatal antibiotics, gestational maturity, early colonisation, breastfeeding initiation and routine vaccination. Postnatal factors include eczema, skin barrier inflammation, the timing and regularity of allergen introduction, breastfeeding management, infant diet diversity, siblings, daycare, pets, smoke and pollution, physical activity and equitable implementation. The strongest actionable evidence remains eczema-linked risk recognition and timely, sustained oral exposure to peanut and well-cooked egg. Maternal and environmental factors are clinically important, but many are contextual rather than prescriptive; they should guide non-restrictive, non-blaming counselling rather than deterministic risk labelling. Full article

The cooperatives combine a pyramidal structure, in which few members concentrate decision-making power, with ownership dispersed among a broad membership base. This configuration favors adverse selection and moral hazard arising from information asymmetry between managers and stakeholders, which legitimacy theory suggests may be reduced through sustainability disclosure. The study advances the literature by combining three elements not yet integrated in research on cooperatives: the cultural pillar as an autonomous dimension of sustainability, disaggregated analysis by pillar, and risk-adjusted financial performance. The level of sustainability disclosure was analyzed from a stakeholder perspective, along with its association with financial performance. Forty-four expert-validated indicators were applied to 39 cooperatives listed in the 2022 World Cooperative Monitor that published complete reports over the 2020–2022 triennium, resulting in 117 observations, estimated by fixed effects with cluster-robust standard errors. Mean disclosure was 0.52, led by the environmental pillar (0.70), followed by the economic (0.62), social (0.47), and cultural (0.33) pillars. The aggregate index showed no statistically significant association with risk-adjusted financial performance, either for ROA or for ROE, whereas the social dimension remained positive and significant regardless of the metric used. This pattern is consistent with the propositions of legitimacy theory regarding the social pillar, although the underlying mechanisms of information asymmetry reduction and legitimacy strengthening were not directly measured in this study. Full article

The valorization of agro-industrial by-products has gained increasing attention as a sustainable strategy to support circular economy principles and reduce environmental impacts. Spain, the world’s largest olive oil producer, generates substantial amounts of olive pomace (OP), which can represent up to 80% of the processed olive mass. Due to their hydrophilic nature, approximately 98% of olive phenolic compounds remain in OP after oil extraction, making this by-product a valuable source of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial, and cardioprotective properties. Numerous extraction strategies have been investigated to maximize phenolic recovery while reducing processing costs and environmental impacts. Conventional solvent-based techniques, such as solid–liquid extraction (SLE) and liquid–liquid extraction (LLE), remain widely used, while greener approaches, including microwave-, ultrasound-, supercritical fluid-, pressurized liquid-, and high-pressure-assisted extraction, among others, have gained increasing attention. In addition, deep eutectic solvents (DES) have been applied either alone or in combination with green extraction technologies to enhance extraction efficiency. This review provides a comprehensive overview of extraction techniques for OP valorization and the analytical methodologies used to characterize OP extracts, including spectrophotometric and chromatographic approaches. The biological activities of OP-derived phenolics and their food applications are also discussed, highlighting their valorization potential. Full article

Enzymatic browning, primarily catalyzed by polyphenol oxidase (PPO), is a major cause of postharvest losses and quality degradation in fresh-cut fruit and vegetable processing. To control this browning, conventional methods such as sulfite treatment and thermal inactivation have been widely used, yet they increasingly face safety, sensory, and regulatory concerns. Because of this, more attention has been directed toward natural PPO inhibitors from agro-industrial by-products as safer, value-added alternatives. However, current knowledge of PPO inhibition mechanisms and rational inhibitor discovery remains fragmented across the literature, limiting the development of effective and sustainable anti-browning approaches. To address this gap, this review presents an integrated framework that covers (i) the structural and kinetic basis of PPO catalysis at the binuclear copper active site; (ii) the mechanistic classification of reversible and irreversible inhibitors, together with kinetic characterization using IC₅₀, K_i, and nonlinear regression approaches; (iii) computational screening strategies, including molecular docking and molecular dynamics simulations as modern tools for predicting enzyme–inhibitor interactions and prioritizing candidate inhibitors; and (iv) the potential of agro-industrial by-products as renewable sources of natural PPO inhibitors for extending the shelf life of fresh-cut produce. Through this framework, this review provides an integrated perspective to support the rational evaluation and future development of effective, sustainable PPO inhibitors for food processing. Full article

Quality control in green coffee bean production is critical for food safety and economic sustainability. A persistent gap in existing automated inspection systems is their inability to capture both sides of each bean, which leads to systematic under-detection of surface defects. This work presents three contributions: (i) a novel mechatronic apparatus that mechanically guarantees dual-sided imaging of every bean, (ii) a public 12-class dataset of green coffee bean defects, and (iii) an embedded, real-time inspection pipeline validated on low-cost hardware. The apparatus sequentially presents each bean, from a standard 350 g sample, to two 16-megapixel cameras under controlled LED illumination. A dataset of 9600 images spanning 12 classes (11 defects and 1 normal) was generated from expert-classified samples and enriched through data augmentation. Four convolutional neural network (CNN) architectures, VGG-16, VGG-19, ResNet-50 and YOLOv8, were trained and benchmarked using precision, recall, F1-score and mean average precision. YOLOv8 achieved the best overall performance, with a precision of 97.4%, a recall of 99.6%, an F1-score of 0.930 and a mean average precision of 96.5%, outperforming VGG-16 (accuracy 86.07%), VGG-19 (accuracy 67.03%) and ResNet-50 (accuracy 87.76%). Dual-sided acquisition raised mean per-class detection accuracy from 0.727 to 0.908, a relative gain of 25.7% over an equivalent single-sided configuration. Deployed in real-time “track” mode on a Raspberry Pi 4, the system simultaneously classifies defects and counts beans by category, processing a 350 g sample in approximately 38 min. Combining mechanical innovation with lightweight deep learning enables practical, scalable, and cost-effective quality control for laboratories specialized in coffee analysis. Full article

Maintaining sustainable production of healthy and nutritious food represents a major global challenge. Cultivated meat could reduce the environmental impact of livestock production, but it remains costly, largely due to the expense of fetal bovine serum, a common cell culture media supplement. Algal and cyanobacterial proteins, particularly phycobiliproteins, represent promising sustainable sources of potentially bioactive peptides that may contribute to the development of serum-reduced or serum-free culture media. This study investigated the potential of hydrolysates prepared using the protein extracts of the red alga Porphyra haitanensis and the cyanobacterium Arthrospira platensis as functional supplements for cell cultivation in a low-serum environment. Twelve hydrolysates were generated by treating protein extracts from Arthrospira platensis and Porphyra haitanensis with six proteolytic enzymes: pepsin, trypsin, chymotrypsin, subtilisin, thermolysin and proteinase K. The effects of these hydrolysates were studied in three cell models of mammalian (CHO-K1), avian (QM7), and fish (MACK1) origin. The effects of hydrolysates on cell viability, proliferation, and antioxidant activity were evaluated. A. platensis–chymotrypsin hydrolysate significantly improved QM7 cell viability compared to control in both 80% reduced-serum and tryptose phosphate broth conditions (p ≤ 0.0001). Additionally, peptide composition analysis and identification of amino acid sequences provided preliminary insights into potentially bioactive chromopeptides associated with the maintenance and promotion of cellular growth. Algae/cyanobacteria-derived hydrolysates positively regulated cell viability and proliferation in serum-reduced conditions, although effects were dependent on the algal species, enzymatic treatment, and cell type. These findings highlight their potential as sustainable supplements for alternative cell culture media. Full article

Maize (Zea mays L.) landraces are rich sources of genetic and nutritional diversity, yet their compositional traits remain undercharacterized compared to modern hybrids. This study compiled and analyzed data from 1554 maize samples—995 landraces and 559 hybrids—evaluated at the CIMMYT Maize Quality Laboratory over more than a decade. Traits assessed included kernel structure, macronutrients, iron, zinc, carotenoids, and anthocyanins. Landraces showed greater variability and higher average concentrations of protein (10.88 vs. 9.92%), iron (17.63 vs. 13.91 mg kg⁻¹), zinc (22.70 vs. 18.98 mg kg⁻¹), and anthocyanins, whereas hybrids, particularly those developed through provitamin A biofortification programs, exhibited higher average provitamin A (3.33 vs. 1.84 µg g⁻¹) and total carotenoid concentrations. Correlations between kernel structural components and nutrient composition highlighted the structural basis of grain quality variation. These findings demonstrate that landraces and hybrids provide complementary nutritional profiles that can jointly support breeding, food innovation, and sustainable food systems. Full article

The development of sustainable, nutrient-dense food systems requires a comprehensive understanding of how microalgae influence the mechanical properties of starch hydrogels. While certain microalgae are common food additives, a critical research gap remains regarding their effect on the rheological behavior and structural integrity of potato starch gels specifically. This study addressed this gap by evaluating the mechanical, microstructural and optical impacts of Arthrospira platensis (commonly known as Spirulina) and Chlorella vulgaris at 0.5%, 1% and 2% (w/w). Utilizing steady-shear flow tests, colorimetry, NIR spectroscopy and microscopy, we characterized changes in steady rheological parameters, color, chemical changes and microstructure of fortified hydrogels. Results indicated that filamentous Arthrospira platensis reinforces the matrix, significantly increasing yield stress from 10.2 Pa in the control to 32.4 Pa at 2% inclusion. In contrast, spherical Chlorella vulgaris appears to act as a structural filler, reducing yield stress to 4.8 Pa at 2%. Microscopy confirmed these morphological influences, showing Arthrospira platensis filaments entangling granules while Chlorella cells integrated into inter-granular spaces. Colorimetry revealed significant darkening (L* decreased from 31.59 to 18.54 at 2% Spirulina addition) and significant greening (p < 0.05). NIR spectroscopy demonstrated potential physical interactions via vibrational markers at 5172 cm⁻¹ and 5646 cm⁻¹, indicating water matrix redistribution within the system. This research demonstrates how incorporating Spirulina and Chlorella vulgaris provides a viable approach for modifying the physical properties of starch-based matrices. The findings indicate that Spirulina enhances flow resistance and structural stability under steady shear, whereas Chlorella vulgaris reduces flow barriers, thereby increasing the spreadability of these composite food systems. Full article

Ecuadorian aquaculture plays an increasingly important role in food security, providing a sustainable complement to capture fisheries. Manabí constitutes a key economic sector that contributes to employment generation, income diversification, and supply of high-quality protein. However, the sector’s heterogeneity and limited legal registration of enterprises hinder the development of policies and targeted interventions. This study characterizes aquaculture enterprises in Manabí, integrating productive, technological, and socio-economic variables obtained from field surveys. Three distinct entrepreneurship patterns were identified: small-scale, informal farms cultivating native species with low technology but characteristics that may contribute to socio-ecological resilience; semi-formal producers operating with intermediate technology and mixed species; and freshwater shrimp farms operating at the largest relative scale within the sample, though still small-scale and family-based, with moderate technological development and market integration limited mainly to local and regional markets. The typology focused on a gradient in formalization, technological sophistication, and market orientation among enterprises. These findings provide a comprehensive understanding of aquaculture diversity in Manabí and offer a practical framework for differentiated policy design using a Strategic Producer Positioning Map. Strengthening formalization, technological innovation, environmental management, and social inclusion is essential to enhance the sector’s sustainability and competitiveness, contributing to sustainable blue economy development in coastal Ecuador. Full article

Freshwater scarcity can weaken water governance when hydrological pressure interacts with intensive agricultural demand, regulatory weakness, and political conflict. This research evaluates whether Irrigation District 005 (IR 005) in Chihuahua, northern Mexico, demonstrates local water-governance fragility across three domains: public security, the rule of law, and the ability to sustain water access and food production. A mixed-methods approach integrates legal and human rights documentation, institutional records, published studies, and a structured media review with hydrological, agricultural, climatic, and reservoir data. Water balances were analysed for 1998–2023, precipitation trends for 1980–2020, and crop water requirements were estimated using the Food and Agriculture Organization’s Irrigation and Drainage Paper No. 56 (FAO-56) Penman–Monteith framework, the crop coefficient (Kc), the water-stress coefficient (Ks), the United States Soil Conservation Service (SCS) Curve Number method, and application-efficiency assumptions. The 2020 water conflict resulted in fatalities, injuries, arrests, and documented human rights violations. Rule-of-law capacity was further diminished by unauthorised withdrawals, cultivation beyond authorised irrigation plans, and limited enforcement. The annual water balance shifted to persistent deficits after 2016, reaching an estimated deficit of 2268 cubic hectometres (hm³) in 2020. Annual precipitation did not exhibit a statistically significant monotonic decline during 1980–2020 (Mann–Kendall Z = −0.79, τ = −0.0878, p = 0.4251; Sen’s slope = −1.1628 mm yr⁻¹; Mann–Whitney p = 0.5313), indicating that recent stress is more closely linked to production scale, crop mix, governance conditions, and irrigation efficiency than to a long-term reduction in rainfall. Sensitivity analysis revealed that ±15% changes in Kc and Ks altered gross water requirements by approximately ±16–17%, while equivalent changes in effective precipitation produced changes of only 1–3%. These results demonstrate heightened water-governance fragility resulting from mutually reinforcing hydrological, institutional, and conflict-related pressures. Future research should refine locally calibrated water-demand parameters and develop reproducible monitoring systems that combine hydrological, institutional, satellite, and participatory data to support anticipatory, transparent, and rights-based water governance. Full article

Natural products derived from plants and microorganisms remain promising alternatives to synthetic chemicals and conventional substances across a wide range of fields, including pharmaceuticals, agriculture, and industry. They also play an important role in the development of sustainable biopesticides and biofertilizers. Chemically, natural products can be grouped into overlapping structural and biosynthetic categories, including alkaloids, terpenoids, flavonoids, glycosides, saponins, and polyketides. Each group reflects distinct chemical characteristics and associated biological functions. This overview highlights the main findings from the Molecules Special Issue: “Natural Products: Isolation, Analysis and Biological Activity, 2nd Edition”, focusing on three categories: (i) plant protection, plant health, and plant biotechnology; (ii) functional foods and health-promoting formulations; and (iii) therapeutic natural products and derivatives. Exploring the mechanisms of action of natural products is essential for ensuring safety and optimizing sustainable resource use. This understanding drives critical innovations across drug discovery and materials science. Ultimately, such research reinforces their cross-disciplinary significance for advancing human health and promoting environmental sustainability. Full article

This work reviews the strategic valorization of industrial by-products as sustainable sources of biopolymers and bioactive compounds, promoting a circular economy through the efficient use of renewable resources and reducing waste generation. These strategies contribute to lowering the carbon footprint of conventional packaging and plasticulture while supporting more resilient and diverse agriculture systems. Special emphasis is placed on processing roots and tubers as renewable raw materials for the production of biodegradable films for agronomic applications as eco-friendly alternatives to petroleum-based plastics and contributing to soil and ecosystem protection. Additionally, the incorporation of by-products from yerba mate (Ilex paraguariensis) demonstrate significant potential as both matrix-forming and filler materials in biodegradable composites while also providing antioxidant activity and pH-sensing capacity. This sustainable framework is further expanded through the utilization of non-traditional species like rosehip (Rosa rubiginosa), Aloe vera (Aloe barbadensis), and topinambur (Helianthus tuberosus), which provide versatile functional matrices and bioactive compounds. Finally, the development of active and intelligent food packaging is addressed. Extracting natural pH-sensitive pigments from red cabbage and topinambur flowers enables the formulation of eco-friendly inks for real-time freshness monitoring. Ultimately, integrating these waste streams drives technological disruption, scaling sustainable, tailored solutions for global industry needs. Full article