Search Results (598)

Dust pollution induced by blasting during tunnel construction via the drill-and-blast method poses a severe threat to workers’ health and construction safety. To address this issue, a wet chord grid dust removal and purification device adaptable to deep-buried long tunnels was developed in this study. The device integrates dust control and removal functions, featuring mobility, high purification efficiency, and water recycling capability. Through experimental tests, the optimal operating parameters of the system were determined: the dust removal efficiency reached a peak of 94.3% (laboratory optimal value from the basic parameter optimization test) when the frequency of the extraction axial flow fan was set to 30 Hz and the cross-sectional wind speed of the chord grid reached 3.34 m/s. The circulating water tank achieved the optimal water treatment performance under the conditions of a relative buried depth of 0.42 for the water inlet, a volume ratio of 1:2 for the sedimentation area to the clear water area, and a relative baffle height of 0.65. Numerical simulations based on CFD software (2021) revealed that the on-site dust removal efficiency of the device reached 79.86% and 87.9% under the working conditions where the tunnel face was 10 m and 100 m away from the connecting passage, respectively, which are in good agreement with the field measurement results. In the practical application at the Shierpo Tunnel of the Guangxi Tianba Expressway, the device achieved an average total dust removal efficiency of 78.4%, with 81.2% removal efficiency for PM₁₀ and 76.5% for PM_2.5, demonstrating excellent engineering applicability and dust removal performance for respirable dust. This study provides effective technical support and a theoretical basis for improving the construction environment of drill-and-blast tunnels. Full article

Elevated temperatures are frequently encountered in numerous occupational settings such as iron and steel foundries, non-ferrous metal foundries, brick and ceramic manufacturing plants, glass production facilities, rubber factories, electrical power plants, bakeries, laundries, chemical processing sites, mining operations, smelting plants, and steam tunnels. Employees working in these environments are at risk of developing various health issues and injuries, including ocular complications, due to prolonged exposure to heat and the physical demands of handling heavy materials. This study focuses on examining the pressure within the eye’s anterior chamber, referred to as Intraocular Pressure (IOP), and its association with the cornea’s biomechanical characteristics, with particular attention to corneal temperature. Our methodology is grounded in the principles of the first law of thermodynamics. The findings reveal a link between the temperature of the eye’s anterior chamber and the biomechanical behaviour of the cornea. Specifically, IOP serves as an indicator of the cornea’s elasticity and its optical properties as influenced by temperature variations. We investigated how the cornea’s elastic energy, or the work it performs, varies with temperature changes. The results show that an increase in temperature corresponds to a reduction in the work exerted by the cornea. The corneal temperature is affected by both the ambient environment and the temperature of the aqueous humour within the anterior chamber. This indicates a relationship between the mechanical work done by the cornea and the pressure exerted by the fluid in the eye’s front segment. Furthermore, our study identified a correlation between corneal thickness and IOP, which our modelling approach successfully quantifies. Utilizing the first law of thermodynamics, we calculated the work performed by the anterior chamber against the cornea’s internal surface. Temperature fluctuations influence the secretion, drainage, and flow characteristics of the aqueous humour, thereby impacting IOP and associated ocular conditions. These insights are valuable for devising strategies aimed at preventing eye injuries among workers exposed to high-temperature environments. Full article

To address the challenge of surrounding rock instability in deep-buried tunnels crossing fractured fault zones, this study focuses on the Xigu Tunnel of the Lanzhou–Hezuo Railway. A combination of laboratory triaxial tests, an optimized multi-source advanced geological prediction workflow, and a site-specific parameter-weakened Mohr–Coulomb numerical simulation is employed to systematically reveal the physical–mechanical properties, spatial distribution, and deformation response of fractured rock masses under excavation-induced disturbance. The triaxial test results show that the average peak strength of the surrounding rock reaches 149.04 MPa; however, significant variability is observed among samples, and the failure mode exhibits a typical brittle–shear composite feature. The measured cohesion and internal friction angle are 20.57 MPa and 49.91°, respectively, indicating high intrinsic strength of individual rock blocks. Nevertheless, due to the presence of densely developed joints and crushed structures, the overall mass is loose and highly sensitive to dynamic disturbances such as blasting and excavation, revealing a unique mechanical paradox of high-strength rock blocks with low overall rock mass stability in deep-buried fractured zones. Joint TSP (Tunnel Seismic Prediction Ahead) and ground-penetrating radar (GPR) prediction reveals decreased P-wave velocity, increased Poisson’s ratio, and intensive seismic reflection interfaces; a quantitative index system for identifying the boundaries of narrow deep-buried fractured zones is proposed based on these geophysical characteristics. Combined with geological face mapping, these results confirm the existence of a highly fractured zone approximately 130 m in width, characterized by well-developed joints, heterogeneous mechanical properties, and localized risks of blockfall and groundwater ingress. The developed numerical model, with parameters weakened based on triaxial test and geological prediction data, effectively reproduces the deformation law of the fractured zone, and the simulation results agree well with field monitoring data, with peak displacement concentrated at section DK4 + 595, thus accurately identifying the center of the fractured belt as a key engineering validation result of the integrated technical framework. During construction, based on the identified spatial characteristics of the fractured zone and the proposed targeted support insight, enhanced dynamic monitoring and targeted support measures at the fractured zone center are required to ensure structural safety and long-term stability of the tunnel. This study develops an integrated engineering-oriented technical framework for deep-buried tunnels crossing narrow fractured zones, and provides novel mechanical insights and quantitative identification indices for such complex geological engineering scenarios. Full article

Electron transfer is one of the most essential processes in biological systems. Redox reactions, either directly or indirectly, drive the main ATP-synthesizing pathways, especially those relying on a chemiosmotic mechanism, and as such, they are fundamental to photosynthesis and respiration. During biochemical redox reactions, electrons are transferred from a low-potential donor to a high-potential acceptor, mainly affecting the oxidation state of carbon atoms. The mechanism of electron transfer remains an intriguing enigma because of the wave-particle duality of subatomic particles. According to the biophysical conditions, electrons can be transferred by quantum tunneling or hopping from one redox site to another. While the driving force is always the electrochemical potential, a particularly interesting case is reversible electron bifurcation, where downhill (exergonic) redox reactions are coupled with uphill (endergonic) reactions by splitting the electrons of a two-electron donor. Here, we aim to discuss these different mechanisms in a comprehensive review accessible to students, teachers, and researchers in biological sciences. Full article

Underground metro tunnel failures in recent years have caused significant economic losses and posed serious risks to surface structures, highlighting the importance of accurately predicting tunnelling-induced ground deformations. Surface settlements occurring during TBM excavation may adversely affect existing infrastructure, particularly in sensitive urban areas. This study evaluates surface deformations induced by a TBM-driven metro tunnel as a case study, explicitly considering tunnel–structure interaction at locations where piled bridge piers are present. Due to site sensitivity, topographic monitoring was conducted during TBM passage, and measured settlement data were used for assessment. Settlement analyses were performed using the Peck (1969) empirical method and finite element modelling in Plaxis. Two constitutive soil models, Mohr–Coulomb (MC) and Hardening Soil (HS), were adopted to compare their predictive performance. The results show that the MC model predicts the highest surface settlements, whereas the Peck (1969) method provides results close to those obtained with the HS model, despite not explicitly incorporating structural loads. From the finite element tunnel models, it was determined—particularly from the two coordinate routes—that the HS model achieved prediction accuracy of up to approximately 95% compared to the measured values. Overall, the Peck approach and the HS model yielded more consistent predictions than the MC model for the investigated conditions, emphasizing the importance of appropriate soil model selection in finite element analyses of tunnelling-induced settlements. Full article

Background/Objectives: To evaluate the efficacy of platelet-rich plasma (PRP) as an adjunctive treatment in anterior cruciate ligament reconstruction (ACLR) and its impact on key clinical outcomes. Methods: A systematic search was conducted across five databases until 11 November 2024, including 33 randomized controlled trials (RCTs) that investigated PRP in ACLR. Outcomes analyzed included ligamentization (MRI hypointensity grades), pain VAS scores, functional scores (IKDC, Lysholm, Tegner), knee stability (KT-1000 arthrometer), and tunnel characteristics. Subgroup analyses were performed based on PRP application site, graft type, risk of bias, and follow-up duration. Results: PRP significantly enhanced ligamentization, particularly at 12 months, with marked reductions in MRI hypointensity grades. Patellar tendon grafts demonstrated the most substantial benefits. PRP also significantly reduced postoperative pain, with effects most pronounced in the early recovery period (1–9 months). However, the analgesic benefits diminished over time. Improvements in IKDC scores were observed only in studies with a high risk of bias, while Lysholm and Tegner scores showed no consistent differences between PRP and controls. Knee stability improved significantly with PRP, but this effect was limited to early follow-up periods (3 months). The heterogeneity in PRP preparation methods, application protocols, and patient populations limited the generalizability of the findings. Conclusions: PRP enhances ligamentization and provides short-term pain relief and stability benefits in ACLR. However, its impact on long-term functional recovery and other clinical outcomes remains limited and inconsistent. Standardization of PRP protocols and further high-quality research are necessary to refine its application and therapeutic potential. Full article

More and more urban underpass tunnels are being constructed to alleviate traffic congestion; however, for this type of underground structure, the soil–structure interaction mechanisms under earthquake loading remain unclear, and dedicated advice and guidance for their seismic design are still lacking. This paper endeavors to investigate the dynamic interaction mechanisms of an underpass tunnel and surrounding soft ground using the finite element (FE) method. Firstly, the accuracy of the FE model in reproducing seismic responses of the layered half-space is validated by comparison with results of equivalent linear one-dimensional site response. Then, the dynamic response characteristics of 3D boat-shaped excavation are analyzed to determine the influence of potential local site amplification on the underpass tunnel. Finally, seismic behaviors of open and buried sections of the underpass tunnel are investigated in detail. The results show that under high-intensity rare earthquakes, severe damage occurs at the ceiling slab near the longitudinal beam and at the base of the side wall of the tunnel’s buried section; seismic underpass–site interactions might be influenced the most by the local topography effect of the 3D boat-shaped excavation, as well as a sudden stiffness change between the open and buried sections. Full article

Parasitoids use different signals to locate their hosts, and these signals can modulate their behavioral decisions. Thus, patch selection and foraging in patches with different characteristics depend on their ability to gather and use such information efficiently. In this study, we evaluated whether the parasitoid Eretmocerus eremicus (Hymenoptera: Aphelinidae), a natural enemy of Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) on tomato plants (Solanum lycopersicum), uses scent cues to select and forage in patches that differ in host density and predation risk. Using choice bioassays in a wind tunnel under a continuous airflow, we recorded selection patch and selection time, as well as foraging parameters, including residence time, oviposition events, and attacks. Our results show that E. eremicus discriminated between sites with and without hosts using scent cues, but discrimination between patches with different host numbers was not detected under our assay conditions. It also distinguished between patches with maximum risk and those without risk, but not between subtle differences in risk. These findings suggest that E. eremicus, responded mainly to contrasting olfactory cues rather than to subtle odor differences. From an applied standpoint, our results motivate deeper investigation into how host- and predator-associated olfactory cues could fine-tune parasitoid deployment in biological control. Full article

The mechanical method has become a new construction method for connected aisles in metro tunnels due to its advantages of fast construction speed, high safety, and minimal ground disturbance. During the tunneling process, the interaction mechanism between the composite segment and the shield cutterhead is complex. Taking Shenzhen Metro Line 8 No. 1 Connected Aisle as the research object, a 3D refined model of the shield cutterhead, composite segments and bolt system were built with Abaqus to investigate their dynamic response under cutting. The Drucker–Prager damage model and contact algorithm were introduced to describe the nonlinear behavior of the cutting process. The reliability of the numerical model was verified by concrete cutting tests and on-site Fiber Bragg Grating monitoring, and good agreements were observed. Results show cutterhead cutting first induces circumferential squeezing, then extends longitudinally with a notable time lag, and longitudinal dynamic response is much stronger than transverse. Affected by cutterhead thrust–rotation coupling, cuttable segments have larger displacement with maximum 0.07 mm, forming an asymmetric deformation zone. Ring joint opening follows “a distal attenuation of the opening amount” rule with maximum 0.018 mm, while bolt stress and displacement show “near-end concentration with gradient attenuation”, with longitudinal bolts being more responsive. Mechanical disturbance from small-shield cutting is minimal, with tunnel segment deformation, joint openings, and bolt stress all remaining well below code-specified allowable values. Numerical results show good agreement with field monitoring data of ring joint openings obtained using Fiber Bragg Grating (FBG) sensors, confirming the reliability of the simulation. The results can provide references for structural design and construction parameter optimization of composite segments in a connected aisle. Full article

Peptidyl arginine deiminase 4 (PAD4) is a Ca²⁺-dependent enzyme that catalyzes histone citrullination and plays a central role in chromatin decondensation during neutrophil extracellular trap (NET) formation. Dysregulated PAD4-mediated NETosis contributes to the pathogenesis of diverse inflammatory and immune-related diseases, including autoimmune disorders, cancer, and thrombosis. Although several synthetic PAD4 inhibitors have been developed, their therapeutic application has been limited by issues related to selectivity, irreversible covalent reactivity, and suboptimal pharmacokinetic properties, prompting growing interest in natural products as alternative modulators of PAD4 activity and NETosis. This article presents a structural and mechanistic overview of natural products that target PAD4 and regulate NETosis. Based on enzyme kinetics, structural analyses, and functional validation, natural PAD4 modulators are classified into four categories: (i) active-site-directed inhibitors that bind within the U-shaped substrate tunnel, (ii) mixed and active-site-adjacent inhibitors that engage surface pockets flanking the catalytic site, (iii) allosteric and hybrid modulators that bind to regulatory regions distinct from the active site, and (iv) functionally validated PAD4 binders supported by biophysical and cellular evidence. Integration of structural, biochemical, and cellular data highlights that indirect or noncanonical modes of PAD4 regulation represent biologically coherent strategies for controlling pathological NETosis. Full article

Background: Postoperative wounds may arise from several etiologies, including open partial pedal amputation, postoperative infection, and dehiscence of surgical sites from wound failure or patient compliance issues. If negative pressure wound therapy is the gold standard, its application in the toes area could be challenging, and as a consequence, standard care is most likely used. The control of the wound microenvironment, both in terms of pH levels and presence of reactive oxygen species, is a key part of the normal wound-healing process. This study evaluated the effectiveness of an oxygen-enriched oil-based device (OEOd) in post-surgical diabetic foot ulcers (DFUs). Methods: This prospective controlled comparative pilot study enrolled 40 patients with diabetes mellitus and post-surgical foot wounds (narrow and deep lesions, including tunneling ulcers) treated at the Diabetic Foot Unit of San Donato Hospital, Arezzo (March 2024–April 2025). Patients were allocated into two groups: those treated by the standard wound care (n = 20) and those treated by OEOd (n = 20). The primary outcome was complete wound healing at 16 weeks; other exploratory endpoints were wound area reduction at 4 and 16 weeks, onset of infection, need for re-intervention, and adverse events. Results: Complete wound healing was achieved in 85.0% of OEOd patients versus 45.0% in the control group (p = 0.020). At 16 weeks, wound area reduction was significantly greater in the OEOd group compared with standard therapy (89.8% vs. 64.0%, p = 0.013). Although infection rates (10.0% vs. 35.0%, p = 0.130) and need for re-intervention (0% vs. 25.0%, p = 0.056) did not reach statistical significance, both favored the OEOd group. No adverse events were reported. Conclusions: OEOd significantly improved the chance of healing post-surgery and showed favorable trends in reducing complications, with an excellent safety profile. Larger randomized controlled trials are warranted to confirm these findings and assess long-term outcomes. Full article

Background: Cerliponase alfa is currently the only approved disease-modifying therapy for neuronal ceroid lipofuscinosis type 2 (CLN2) disease and requires lifelong intracerebroventricular (ICV) infusion, traditionally via a scalp-sited ventricular access device (VAD). Chest-sited port (chest port) for intracerebroventricular access using a tunneled central venous access device is described as an alternative, though data remain limited. Methods: We present an anonymized caregiver narrative perspective describing two pediatric patients with CLN2 disease receiving cerliponase alfa infusions via different ICV access strategies: one patient who transitioned from a scalp-based ventricular access device to a chest port and one patient who initiated therapy with a chest port. A semi-structured caregiver interview was used to capture experiential insights related to decision-making, procedural burden, safety considerations, and psychosocial adaptation. Results: The caregiver identified key advantages of chest ports for ICV infusion, including durability of the device, enhanced securement, and smooth long-term routine integration. The transition from a scalp VAD to a chest port was described as proactive, well-coordinated, and associated with high caregiver satisfaction. Noted considerations included increased visibility of the access needle to the child, proximity to oral secretions, and potential misidentification of the port by emergency medical services. Families implemented mitigation strategies through labeling, education, and coordination with the care team. Conclusions: This caregiver-centered case report highlights how access device choice meaningfully shapes treatment burden, safety planning, and daily life for families managing CLN2 disease. As chest-port methodologies become adopted, incorporating caregiver and patient perspectives is essential to developing patient-centered treatment options for long-term intracerebroventricular therapy. Full article

Chemosensory systems are crucial for insect survival, enabling host-seeking, food acquisition, and oviposition site selection. While insect-associated microbes are known to influence host development and immunity, their role in modulating chemosensory behavior remains poorly understood. Here, we show that mono-association with Escherichia coli alters sensory-driven behaviors in both larval and adult axenic Drosophila melanogaster. In larvae, E. coli mono-association altered phototaxis and mechanosensory responses across genotypes, while changes in tunneling and thermosensory behaviors were reduced or absent in ionotropic receptor (IR) co-receptor mutants. In adults, E. coli mono-association increased attraction to fermentation cues (apple cider vinegar, ethanol) and enhanced sucrose consumption in wild-type and Orco-deficient flies, whereas these effects were reduced or absent in IR co-receptor mutants (IR25a⁻ and IR76b⁻). Together, these findings indicate that under defined gnotobiotic conditions, E. coli exposure alters sensory-driven behavioral outputs relative to axenic controls. Effects are reduced or absent in IR co-receptor mutants, consistent with a role for IR pathways in mediating these behavioral shifts. These findings support a role for microbial cues in shaping insect sensory-driven behaviors and highlight the importance of microbial status in interpreting behavioral phenotypes. This work provides a framework for future studies investigating how microbial signals interact with conserved sensory pathways. Full article

In this work, we consider the influence of antisite disorder, e.g., Fe ions on Mo sites, Fe_Mo, and vice versa, Mo_Fe, on the resistivity of strontium ferromolybdate ceramics fabricated by the solid-state reaction method. Strontium ferromolybdate ceramics fabricated via solid-state reactions exhibit a low-temperature minimum resistivity owing to the interplay between the bulk metallic resistivity of the grains, which increases with temperature and becomes dominant at higher temperatures, and an intergrain tunneling mechanism of charge carrier conduction, which leads to a decrease in conductivity with decreasing temperature in the low-temperature region. The parameters of the bulk metallic resistivity and fluctuation-induced intergrain tunneling were determined by fitting the experimental data to these resistivity models. The impact of antisite disorder on the resistivity parameters was considered. It turns out that antisite disorder affects the effective barrier height of intergrain tunneling and the effective values of the barrier width and the barrier area. Disorder increases the effective barrier height for intergrain tunneling, increases its barrier width, and decreases the effective barrier area of nanosized barriers. The results are discussed using experimental data available in the literature. Full article

Background/Objectives: The anterior cruciate ligament (ACL) plays a key role in knee stability, biomechanics, and proprioception, and is one of the most frequently injured and reconstructed ligaments in both athletes and the general population. The anatomical placement of femoral and tibial tunnels close to the native ACL insertion sites is critical for long-term clinical outcomes and graft survival. This study aimed to define sagittal and coronal ACL alignment and tibial footprint morphology on magnetic resonance imaging (MRI) in healthy knees, to explore sex- and side-related differences, and to provide population-specific reference values. Methods: In this retrospective cross-sectional study, knee MRIs acquired between 2018 and 2021 were screened, and knees with an intact ACL and without deformity or joint pathology that could alter alignment were included. After applying inclusion and exclusion criteria, 636 knees (320 right, 316 left) from 545 individuals (338 women, 298 men; 15–80 years, mean age 34.87 ± 11.65 years) were analyzed. On sagittal images, the sagittal ACL angle (S-ANGLE) was measured on the slice where the ligament appeared maximally visualized. The midpoints of the ACL were identified on two adjacent sagittal slices, and a line drawn through these midpoints was used to represent the central axis of the ligament; the angle between this line and the tibial plateau was recorded as the S-ANGLE. For anteroposterior localization of the tibial footprint, an anteroposterior reference distance (S-long) was defined as the length measured parallel to the tibial plateau, extending from the midpoint of the tibial tuberosity (corresponding to the insertion site of the patellar ligament and used as a topographic anterior landmark) toward the posterior aspect of the proximal tibia. A perpendicular line was drawn from the anterior end of S-long to establish the anterior reference boundary. The distance from this anterior reference line to the midpoint of the ACL tibial footprint along the same anteroposterior axis was defined as S-short. The sagittal footprint percentage (S-PERCENTAGE) was calculated as (S-short/S-long) × 100, representing the size-normalized sagittal anteroposterior position of the ACL tibial footprint midpoint. On coronal images, the ACL–tibial plateau angle (C-ANGLE), mediolateral tibial length (C-LONG), and distance from the medial edge to the ACL insertion (C-short) were obtained; C-PERCENTAGE was calculated analogously. Medial mechanical proximal tibial angle (mMPTA) was used to confirm physiological coronal alignment. Non-parametric tests were applied, with p < 0.05 considered statistically significant. Results: Women had significantly greater sagittal ACL angles than men, whereas anteroposterior distances measured from the midpoint of the tibial tuberosity (used as an anterior topographic landmark) and oriented parallel to the tibial plateau (S-LONG) and mediolateral tibial lengths (C-LONG) and absolute distances to the ACL tibial footprint were larger in men. In contrast, normalized sagittal and coronal footprint percentages (S-PERCENTAGE, C-PERCENTAGE) did not differ meaningfully between sexes, indicating the preservation of the relative ACL tibial insertion site despite size differences. Small but statistically significant side-to-side differences were observed in some coronal parameters; however, absolute differences were small and did not substantially modify the overall alignment pattern. Conclusions: This study provides large-sample, population-specific reference values for ACL orientation and tibial footprint location in both sagittal and coronal planes in healthy knees. The combination of higher sagittal ACL angles and shorter anteroposterior distances reference measured from the midpoint of the tibial tuberosity and oriented parallel to the tibial plateau (S-LONG) in women may represent a structural substrate contributing to the higher ACL injury rates reported in females. The morphometric data presented here may assist in individualized ACL reconstruction planning, MRI-based assessment of tibial tunnel position, and the design of knee-related biomedical implants and devices. Full article