Search Results (218)

High-Resolution Site Characterization (HRSC) offers a promising approach to delineate spatially heterogeneous contamination in complex petrochemical sites, overcoming limitations of conventional discrete sampling. This study implemented an integrated HRSC framework combining surface soil microbial metabolic gas/functional gene detection, geophysical surveys (time-domain electromagnetics and ground-penetrating radar), and Membrane Interface Probe (MIP) sensing at a petrochemical facility in southern China. Results identified composite contamination (aromatic hydrocarbons, short-chain petroleum hydrocarbons, alkanes) primarily concentrated at 5–9 m depth, with a heavily contaminated zone of 1163 m² and a total influence area of 17,724 m². The contamination plume showed high spatial correlation with an underground wastewater storage pond, confirmed as the primary leakage source. Post-remediation monitoring indicated restoration of natural groundwater flow and reduced contaminant concentrations. Compared to traditional drilling, the HRSC approach improved resolution from meter to centimeter scale, reduced investigation time by 75%, and lowered overall costs by >30% through targeted sampling and real-time data acquisition. This study validates HRSC as an efficient, accurate, and cost-effective strategy for contamination delineation and source identification in operational industrial sites, supporting precise remediation and site redevelopment. Full article

Hospital wastewater treatment efficacy is conventionally assessed by total antibiotic resistance gene (ARG) abundance; however, whether this metric accurately reflects biosafety risk remains poorly defined. Using a one-year longitudinal metagenomic survey (bimonthly sampling; n = 18 per group), we simultaneously profiled the resistome, virulome, and mobilome of hospital wastewater influent and effluent; stratified functional gene abundances by genomic origin; quantified ARG–mobile genetic element (MGE) colocalization; and characterized multicategory gene cocarriage across the 15 most abundant pathogenic species. Although the abundance of total strict ARGs decreased significantly in the effluent (p = 0.038), the abundances of metal resistance genes and virulence factors increased concurrently (both p < 0.01), and 8 of the 20 ARG subtypes were enriched rather than removed. This decline was driven exclusively by a reduction in the number of plasmid-encoded ARGs (p < 0.001), whereas genes encoding chromosomal virulence factors, metal resistance genes, biocide resistance genes, and MGEs were significantly enriched in the effluent (all p < 0.05). The normalized ARG–MGE colocalization rate was significantly greater in the effluent (p = 0.028), with a concurrent shift toward transposase-mediated chromosomal mobilization. Pathogen-associated metagenomic assemblies of clinically relevant species exhibited synchronous multicategory resistance coenrichment in the effluent, which is consistent with coselection under antibiotic, biocide, and metal pressures. Total ARG abundance is fundamentally decoupled from biosafety risk in treated hospital wastewater, warranting integrated surveillance beyond ARG-centric metrics. Full article

Ecological risk assessment of ecosystems facing anthropogenic pressures informs coastal management. This study evaluated the ecological risk of ecosystems in two coastal municipalities in the Gulf of California, Mexico. The coastal area under study spans 175 km of coastline and includes various ecosystems, as well as the cities of Guaymas and Empalme (~160,000 inhabitants). Ecological risk was assessed by surveying the opinions of experts on local and global activities and influences (climate change), the ecological consequences of hazards, and the resilience (fragmentation) and natural recovery of ecosystems. In addition, potential synergies between human activities and the effects of climate change were identified. The results showed that the main threats are discharges of raw or poorly treated wastewater into the sea, the generation and dumping of garbage, and illegal fishing. Wastewater discharges represent the local threat that interacts most intensively with the effects of climate change. Mangroves, coastal water bodies, and rocky shores face the greatest ecological risk due to continuous exposure to anthropogenic threats, poorly planned urban growth, and industrial development. Approximately 20% of the coastal zone is estimated to correspond to the metropolitan areas of Guaymas and Empalme, where the greatest ecological risk occurs, and these represent opportunities to promote coastal management processes aimed at ecosystem restoration and planned urban development to prevent the loss of coastal ecosystem functions and the services they provide to society. Full article

The increasing prevalence of cancer in Thailand over the past decade has resulted in a substantial rise in the use of anticancer drugs, which are eventually discharged into municipal wastewater through hospital and domestic effluents. The inability of conventional wastewater treatment systems to completely remove these pharmaceuticals has been widely reported. The continuous release of these emerging anticancer agents into aquatic environments reduces water quality and threatens biodiversity. Even at trace levels, these compounds may act as persistent pollutants capable of impairing ecosystem. This study investigated the occurrence and concentration levels of three widely used chemotherapeutic agents including cyclophosphamide (COP), doxorubicin (DOX), and vincristine (VIN) in Bangkok’s municipal wastewater to evaluate their potential environmental risks. Thirty-two influent and effluent wastewater samples were collected from eight large-scale wastewater treatment plants (WWTPs) from October 2024 to January 2025. Samples were processed using solid-phase extraction (SPE) and analyzed by liquid chromatography–triple quadrupole mass spectrometry (LC–MS/MS). The analytical method demonstrated high precision and reproducibility, with relative standard deviations (%RSD) below the 20% acceptance limit for all compounds. Method accuracy ranged from 81.84% to 107.21%. Results showed the presence of only COP in almost influent and effluent at levels ranging from 0.26 to 2.06 µg/L. In contrast, DOX and VIN levels remained consistently below the limits of quantitation (LOQ) in all WWTP samples. This study establishes the first baseline for COP, DOX, and VIN contamination in Bangkok’s municipal wastewater. Notably, the residue of COP in wastewater suggests that current wastewater treatment facilities in Thailand are insufficient for its removal, posing a potential long-term risk to local aquatic ecosystems. Full article

In the United States, most urban areas are served by sewers and wastewater treatment plants, while septic systems remain common in rural regions, in lower-density communities, and in areas with lower levels of public services. The policy frameworks for septic system management are complex and variable, involving multiple key actors and levels of government and varying considerably from place to place. This research seeks to characterize the septic system management practices in two New York State watersheds: The first study area is located in central New York (the Lower Seneca River watershed) and comprises communities with greater reliance on surface water, and the second is in eastern New York on Long Island (Peconic Estuary Watershed), where groundwater is the primary source of drinking water. Since homeowner practices play a central role in outcomes, we also investigate homeowners’ understandings of their septic systems (also called onsite wastewater treatment systems, or OWTS). The methods used include policy analysis as well as qualitative research methods (interviews, focus groups, and survey research) to characterize homeowners’ perceptions and understandings, including their awareness of grant programs for septic system upgrades and replacement. The results show that most septic system owners hold only partial understandings of their systems. Their awareness of the connections between septic system management and groundwater protection is limited, with noted differences across watersheds. The study findings can inform future planning initiatives, as they illustrate the value of placing community water system understanding at the forefront of outreach efforts. Full article

Antimicrobial resistance (AMR) has become one of the top ten global public health threats. Many countries have recognized the societal and economic burden of AMR. AMR has reduced the effectiveness of antimicrobial therapies, and this results in high mortality, morbidity, and health care expenditure. Like all the other developing countries, South Africa (SA) falls under the same ambiguous management system of antimicrobials. A lot of research focused on the global public health threat “AMR”. However, studies on AMR in wastewater are not yet enough, even though they are beginning to gain momentum. This paper highlights the imperatives of surveying AMR pathogens in wastewater since wastewaters are consecrated as hotspots for the dissemination and propagation of AMR genes. RNA was extracted from the untreated wastewater samples collected from the Tshwane district in Gauteng province, SA. Metatranscriptomics analysis was proposed for the analysis and profiling of AMR genes present in the wastewater. A total of 39 AMR gene families and 39 AMR drug classes were detected across 17 samples. The Metatranscriptomics approach discussed in this paper demonstrates the importance of wastewater surveillance, as it can be used as an early detecting system for communicable diseases and for monitoring wastewater. Full article

Cerium dioxide (CeO₂) has emerged as a structurally versatile oxide for dye-wastewater purification because its architecture, porosity, and surface accessibility can be tuned over a wide range while maintaining good chemical stability and environmental compatibility. Recent studies show that template-free or low-template routes can generate porous, mesoporous, multilayered, and flower-like CeO₂ architectures with rapid dye uptake and, in some systems, adsorption-assisted photocatalytic removal. However, CeO₂-based dye removal has often been discussed either within broad surveys of environmental applications or from composition-centered viewpoints, whereas the more fundamental question is how synthesis route controls architecture formation and how architecture, in turn, governs adsorption and subsequent removal behavior. This mini-review addresses that question from a morphology-centered perspective. It first examines template-free and low-template routes for constructing structured CeO₂, then discusses how porosity, hierarchical assembly, and surface accessibility regulate adsorption kinetics and equilibrium capacity in dye-containing aqueous systems. It further considers adsorption-assisted photocatalytic removal and argues that dark adsorption should be regarded as the structural first step rather than a secondary contribution. On this basis, the review shows that rare-earth doping in these systems is most usefully understood as a secondary tuning strategy that refines an already favorable host architecture by modifying surface interaction, optical response, or reactive-species generation. Overall, the available evidence indicates that CeO₂-based dye-wastewater purification is most meaningfully interpreted through a route–architecture–function framework in which morphology defines the host, adsorption organizes the local reaction environment, and doping serves mainly as structure-assisted tuning. This perspective shifts the design logic of CeO₂ from empirical performance optimization toward rational structure-directed construction of integrated removal platforms. Full article

Background and Objectives: The improper disposal of expired and unused medications (EUM) poses significant environmental and health risks. Discarding EUM in household trash or drains leads to accidental poisoning, illegal trade, and ecosystem contamination. These persistent compounds often resist wastewater treatment, disrupting ecological balance and contributing to antimicrobial resistance, thereby increasing morbidity and mortality rates. This study aims to analyze the knowledge, attitudes and practices (KAP) and related factors of the Mexican population regarding the disposal of EUM. Methods: A cross-sectional, descriptive, and correlational study was conducted via an online survey of adults (18+) from October 2021 to October 2024. Results: Among 6080 participants (95.4% aged 18–59; 65.8% women), a medium level of KAP was observed. Notably, 51.5% did not use specialized disposal containers, only 15.5% knew container locations, and 30.5% correctly identified expiration dates. Significant associations emerged: lower education levels correlated with poorer disposal knowledge, while health-related backgrounds and postgraduate studies linked to positive attitudes and adequate practices. Ordinal logistic regression revealed that being elderly, belonging to a high socioeconomic class, having lower education levels, and lacking health-related studies were significantly associated with poor KAP regarding EUM disposal. Conclusions: Inadequate pharmaceutical disposal in Mexico compromises environmental and public health. Addressing this requires reinforced regulations, professionalized pharmacies, and a comprehensive approach to bridge knowledge gaps. Integrating digital tools—like real-time mapping and QR labeling—with accessible take-back schemes is vital in mitigating hazards and uphold the One Health triad. Full article

The reuse of treated wastewater for agricultural irrigation is increasingly considered a strategic response to water scarcity and climate change, particularly in Mediterranean regions. This study examines the local feasibility and social acceptance of water reuse within the framework of Regulation (EU) 2020/741, focusing on its implementation in Italy. The research combines policy analysis, technical assessment of effluent quality from the GIDA wastewater treatment plant (Prato, Tuscany), GIS-based spatial evaluation, and a mixed-method survey of local agri-food producers. Results show substantial compliance with EU minimum quality requirements, alongside additional constraints arising from national regulatory thresholds. Survey findings reveal cautious but tangible openness among farmers toward reclaimed water use, particularly in response to increasing climate-related pressures. The case of Prato is further analysed within the Prato Circular City and local food policy frameworks, highlighting the role of participatory governance and multi-actor engagement in supporting reuse initiatives. The study contributes empirical evidence on the interaction between EU regulation, national implementation measures, and local socio-institutional conditions shaping peri-urban water reuse systems. Furthermore, it serves as a preliminary framework for future economic feasibility studies and the subsequent regulatory and permitting phases required to operationalize this practice. Full article

Coastal marine sediments and beach sands receive microbial and genetic inputs from wastewater discharge, urban runoff, aquaculture, wildlife, and recreational activity, yet their role as coupled microbial–genetic interfaces linking environmental processes and human exposure remains incompletely synthesized. This review integrates quantitative evidence from culture-based studies, qPCR surveys, metagenomic analyses, and multi-year monitoring investigations focused on coastal sediments and sands. Reported antibiotic resistance gene (ARG) concentrations in coastal sediments reach 2.2 × 10⁹ copies g⁻¹ (wet weight) for sul1 in wastewater-impacted systems, with total ARG abundances commonly ranging from 1.59 × 10⁷ to 2.88 × 10⁸ copies g⁻¹ in effluent-receiving zones and tetM reported at 1.43 × 10⁷ copies g⁻¹. Beach sands contain measurable resistance markers, including intI1 at 9–3823 copies g⁻¹ and blaTEM up to 14 copies g⁻¹ in wet sand. Viable fecal indicator bacteria and pathogens have been cultured directly from sands, including Staphylococcus aureus at 0–8710 CFU g⁻¹ and methicillin-resistant S. aureus at 0–605 CFU g⁻¹. Collectively, the evidence indicates that coastal sediments and sands function as structured microbial and genetic reservoirs requiring integrated assessment of benthic retention, hydrodynamic redistribution, and exposure-relevant interpretation. Full article

Poultry farming is important from a food point of view, as it provides products for the population. Waste resulting from poultry farming and processing is environmentally sensitive, which is why treatment technologies are crucial for sustainability. Conventional pre-treated wastewater originating from the poultry sector, combined with innovative methods of using substances with antimicrobial effects constitute another approach to eliminating health risks. This research aimed to evaluate the behavior of bacterial strains isolated from water samples treated by reverse osmosis (RO), ultrafiltration (UF), and ultraviolet disinfection (UV). To choose our antimicrobial substances, we surveyed the population using the Google Forms platform and the official questionnaire. Using a factorial design, the concentration of antibiotic or antiseptic, as well as the duration of their action on bacterial strains isolated in pure culture, was varied. The optical densities of bacterial cultures reflect the efficiency of treatments with antibacterial or antiseptic substances. Factorial design, corroborating the experimental results, led to the following: 4.84 mg·L⁻¹ antibiotic for 7.6 h and 4.23 mg·L⁻¹ chloramine for 16.23 h. The results obtained from mathematical modeling correspond to a decrease in the bacterial population. In perspective, combining purification technologies with antimicrobial substances, adapted to the volume of water through mathematical modeling, will reduce the risk of microbiological contamination, with bacterial recovery rate values ranging between 0.58 and 1.21 in the case of antiseptic using, and between 0.60 and 0.92 in the case of antibiotic using. Full article

The rapid development of rural and peri-urban areas increases the demand for decentralized wastewater treatment systems. Small wastewater treatment plants (SWTPs) with a capacity below 2000 PE are becoming an important element of local water protection and circular-economy strategies, yet clear guidelines for selecting appropriate technologies are still lacking. This study analyzes the criteria used in decision-making for SWTPs from a multi-stakeholder perspective and evaluates the relative importance of technical, economic, environmental and social factors. The research was conducted in Poland and included a survey of 130 respondents representing six stakeholder groups (officials, operators, designers, contractors, scientists and residents). Respondents allocated weights to four main groups of criteria and assessed eleven detailed parameters on a 1–10 scale. The data were analyzed using descriptive statistics, the Kolmogorov–Smirnov test with the Lilliefors correction to verify distribution assumptions, and the Kruskal–Wallis test to examine differences between stakeholder groups. The results show a consistent hierarchy of criteria, with technical reliability, treatment efficiency and operating costs ranked as the most important factors. Social and environmental aspects were assessed as relevant but secondary. Only minor differences between stakeholder groups were observed. The study highlights the need for integrated, multicriteria approaches in SWTP planning, particularly in dispersed rural areas. The findings may support local authorities, designers and investors in technology selection. The research is limited by the non-probability sampling strategy, the national scope of the dataset and the cross-sectional character of the survey. Full article

Extended-spectrum β-lactamase-producing E. coli (ESBL-EC) threatens public health by driving widespread antimicrobial resistance transmission in environmental and agricultural settings. This study examined the prevalence, genetic determinants, and phylogenetic relationships of ESBL-EC isolated from municipal wastewater treatment plants (WWTPs) and farm environments in southeastern North Carolina. A cross-sectional survey was conducted between May and September 2025 across two WWTPs and two farms (cattle and poultry). We sampled influent and effluent wastewater, plus fecal and water specimens collected from chickens, ducks, and cattle. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disk diffusion method against nine drugs, while PCR and sequencing were used for genotypic characterization. Phylogenetic analysis assessed genetic relatedness among isolates. ESBL-EC was detected in 27.4% (n = 124) of 452 samples, with the highest prevalence in chickens (31.5%), followed by WWTP influents (28.2%), ducks (18.5%), and cattle (12.1%). Dominant resistance genes included blaCMY-2 (71.8%), blaCTX-M-1 and blaOXA (54% each), and blaSHV (29.8%). Co-occurrence of blaCMY-2 with blaCTX-M-1 and blaOXA was observed in poultry isolates. Phylogenetic analysis revealed clonal relatedness between poultry and cattle isolates. These findings highlight poultry as a key reservoir and emphasize the need for One Health surveillance to mitigate cross-reservoir transmission of resistant E. coli. Full article

Background: Hospitals are recognized as point sources of antimicrobials in urban wastewater systems; however, comprehensive evaluations of their discharge profiles have not yet been conducted. Methods: This study presents a multi-site investigation of residual antimicrobial concentrations in effluents from five general hospitals and a commercial facility in the metropolitan area of Japan. Over a 12-week period (December 2023–March 2024), extensive sampling was conducted. Fifteen antimicrobials from multiple classes were quantified using high-throughput analysis. Results: The results revealed consistently higher concentrations in hospital effluents, particularly for levofloxacin, vancomycin, and ampicillin, than in non-clinical sites. Distinct facility-specific and temporal patterns suggest strong links between local prescribing practices and the effluent composition. Some compounds, such as clarithromycin and minocycline, showed dual contributions from both hospital and commercial sources. Conclusions: These findings highlight the need for source-targeted monitoring and antimicrobial pollution control strategies and provide a foundation for expanding surveillance efforts and informing environmental policies related to antimicrobial resistance (AMR). Full article

Understanding hydrochemical evolution and apportioning pollution sources are prerequisites for effective groundwater protection at the regional scale; nevertheless, the governing processes and anthropogenic drivers in arid regions remain poorly constrained. Here, we present a comprehensive geochemical survey of the Datong River Basin, a representative arid catchment in north-western China. Thirty-seven groundwater samples were analyzed with hydrochemical methods and Positive-Matrix Factorization (PMF) to delineate natural controls and contaminant sources. Results showed that the aquifer is dominated by HCO₃–Ca(Mg) water controlled predominantly by silicate and carbonate weathering, modified locally by evapo-concentration and human activities. Water-quality indices classify 70.3% of the samples as excellent, but spatially restricted degradation is evident. PMF resolved three independent sources: a natural end-member enriched in Mn, Na⁺ and Cl⁻; a mixed source reflecting domestic wastewater, agricultural fertilizers and rock weathering; and an industrial source dominated by Fe. The mixed source contributes most major ions and chemical oxygen demand (COD), whereas the industrial source accounts for 75.7% of total Fe. These findings provide a robust scientific basis for groundwater management and pollution mitigation in arid regions under similar hydrogeological settings. Full article