Search Results (18,245)

The study aimed to evaluate the effect of betanin—a bioactive, natural pigment found in beetroot and prickly pear—on cognitive function, motor performance, and neurotransmission in a mouse model of Parkinson’s disease (PD). Aged mice with PD-like symptoms induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) were pretreated with betanin (50 or 100 mg/kg b.w./day) via drinking water. Behavioural tests assessed motor skills, anxiety-related behaviour, and spatial memory. Biochemical analyses of central nervous system structures were conducted using high-performance liquid chromatography (HPLC) to determine neurotransmitter levels and metabolites. Betanin improved motor and cognitive functions in MPTP-treated mice. While learning ability remained unchanged, the 50 mg/kg dose alleviated spatial memory deficits. Biochemically, betanin moderately limited dopamine depletion and significantly influenced dopamine metabolism and serotonin levels. These findings suggest that betanin, as a functional food component, may exert neuroprotective effects and support cognitive and motor function in neurodegenerative conditions such as PD. Full article

Background: An epidemiological survey of Onchocerca volvulus infection and onchocerciasis in Igbo-Eze North Local Government Area, Enugu State, Nigeria, was undertaken to assess its distribution and risks among individuals in the area. Methods: A total of 201 residents who have lived in the study area for at least one year were recruited. At recruitment, they were examined using a rapid assessment method. Their demographic information and risk factors were obtained using a structured questionnaire. Results: The overall prevalence for onchocerciasis was 3.5% (7/201). The prevalence of onchocerciasis was observed to be non-significantly (p = 0.689) different among the studied communities. The sex and age-related prevalence differences were non-significant (p > 0.05); however, onchocerciasis was more prevalent among males and those between 30 and 39 years of age. The prevalence of onchocerciasis was higher (p = 0.001) among farmers/fishermen. The significant risk associated with onchocerciasis is the proximity of the water body to houses (p = 0.034). Conclusions: The onchocerciasis prevalence was low and not dependent on sex or age but rather occupation. Risks to onchocerciasis are environmental and occupational, with chances of scaled up prevalence and burden overtime if unchecked. There is a need for awareness campaigning to enable proper education of the people about onchocerciasis in the area and neighboring communities. Full article

Climatic factors have always played a key role in the construction of food-related architecture: mitigation of outdoor temperatures or winds, adoption of raining waters in the productive processes, etc. However, sometimes, climate change has impacted the profitability of those structures and eventually caused their abandonment. Today, historical food-related architectures are significant elements of local rural heritage, and they are also tangible symbols of all the values connected to the corresponding typical food productions. When the cultural value of rural cultural assets and the historical management of climatic factors coexist, this potential can be investigated, and the results can ultimately be included in the corresponding enhancement processes. To exploit this potential, the paper introduces the theoretical concept of food-related architecture as climatic indicators, with reference to the changes in the climate that have occurred during their construction, as well as their abandonment. According to the thesis of the research, the adoption of the concept of climatic indicators can implement the value of selected minor cultural assets, support sustainable rural regeneration plans and integrate missing historical climate series and data. In the Materials and Methods section, two theoretical charts have been introduced, and the pyramid of the Mediterranean diet was analyzed to allow for the selection of some food-related architectures to test the theoretical approach developed. Then, three Italian case studies have been analyzed: the concept of climate indicators was tested, and some potential focus points of actions connected to this aspect were elucidated. The case studies are the Pietragalla wine district in the Basilicata Region, the Apulian rock-cut oil mills and Mills’s Valley in the Campania Region. Full article

Polymer-based product usage in modern society is increasing day by day. Following usage, these inert products and hydrophobic materials contribute to environmental pollution, often accumulating as litter in ecosystems and contaminating water bodies. The rapid socio-economic development in the Kingdom of Saudi Arabia (KSA) has resulted in a significant increase in waste generation. This study was conducted on the utilization of recycled plastic waste (RPW) polymer along with commercial polymer (CP) for the modification of the local binder. The hot environmental conditions and increased traffic loading are the major reasons for the permanent deformation and thermal cracks on the pavements, which require improved and modified road performance materials. The Ministry of Transport and Logistical Support (MOTLS) in Saudi Arabia, along with other related agencies, spends a substantial amount of money each year on importing modifiers, including chemicals, hydrocarbons, and polymers, for modification purposes. This research was conducted to investigate and utilize available local recycled plastic materials. Comprehensive laboratory experiments were designed and carried out to enhance recycled plastic waste, including low-density polyethylene (rLDPE), high-density polyethylene (rHDPE), and polypropylene (rPP), combined with varying percentages of commercially available polymers such as Styrene-Butadiene-Styrene (SBS) and Polybilt (PB). The results indicated that incorporating recycled plastic waste expanded the binder’s susceptible temperature range from 64 °C to 70 °C, 76 °C, and 82 °C. The resistance to rutting was shown to have significantly improved by the dynamic shear rheometer (DSR) examination. Achieving the objectives of this research, combined with the intangible environmental benefits of utilizing plastic waste, provides a sustainable pavement development option that is also environmentally beneficial. Full article

Soil salinity disrupts rhizosphere interactions, impairing root–microbe symbioses, nutrient uptake, and water relations in onion (Allium cepa L.) and garlic (Allium sativum L.). This study evaluated the efficacy of biofertilizers (Azotobacter chroococcum SARS 10 and Azospirillum lipoferum SP2) and organic amendments (sewage sludge and poultry manure) in salt-affected soils in Kafr El-Sheikh, Egypt. Five treatments were applied: (T1) control (no amendments); (T2) biofertilizer (3 L/ha for onion, 12 L/ha for garlic) + inorganic P (150 kg/ha P₂O₅ for onion, 180 kg/ha for garlic) and K (115 kg/ha K₂SO₄ for onion, 150 kg/ha for garlic); (T3) 50% inorganic N (160 kg/ha for onion, 127.5 kg/ha for garlic) + 50% organic manure (6000 kg/ha for onion, 8438 kg/ha for garlic) + P and K; (T4) biofertilizer + T3; and (T5) conventional inorganic NPK (320 kg/ha N for onion, 255 kg/ha N for garlic + P and K). Soil nutrients (N, P, K), microbial biomass carbon (MBC), dehydrogenase activity, and microbial populations were analyzed using standard protocols. Plant growth (chlorophyll, photosynthetic rate), stress indicators (malondialdehyde, proline), and yield (bulb diameter, fresh yield) were measured. Treatment T4 increased MBC by 30–40%, dehydrogenase activity by 25–35%, available N (39.7 mg/kg for onion, 35.7 mg/kg for garlic), P (17.9 mg/kg for onion), and K (108 mg/kg for garlic). Soil organic matter rose by 8–12%, and cation exchange capacity by 26–36%. Chlorophyll content improved by 25%, malondialdehyde decreased by 20–30%, and fresh yields increased by 20–30% (12.17 tons/ha for garlic). A soybean bioassay confirmed sustained fertility with 20–25% higher dry weight and 30% greater N uptake in T4 plots. These findings highlight biofertilizers and organic amendments as sustainable solutions for Allium productivity in saline rhizospheres. Full article

Due to its efficiency, advanced oxidation processes (AOP), such as photo-Fenton, have become an alternative for removing emerging contaminants like paracetamol. The objective of this work was to perform a life cycle assessment (LCA) according to ISO 14040/44 for a heterogeneous photo-Fenton process catalyzed by Cu/Fe-pillared clays (PILC) for the removal of paracetamol from water. The study covered catalyst synthesis and four treatment scenarios, with inventories built from experimental data and ecoinvent datasets; treatment time was 120 min per functional unit. Environmental impacts for catalyst synthesis were quantified with ReCiPe 2016 (midpoint), while toxicity-related impacts of the degradation stage were assessed with USEtox™ (human carcinogenic toxicity, human non-carcinogenic toxicity, and freshwater ecotoxicity). Catalyst synthesis dominated most midpoint categories, the global warming potential for 1 g of Cu/Fe-PILC was 10.98 kg CO₂ eq. Toxicity results for S4 (photo-Fenton Cu/Fe PILC) showed very low values: 9.73 × 10⁻¹² CTUh for human carcinogenic and 1.29 × 10⁻¹³ CTUh for human non-carcinogenic. Freshwater ecotoxicity ranged from 5.70 × 10⁻⁴ PAF·m³·day at pH 2.7 (≥60 min) to 1.67 × 10⁻⁴ PAF·m³·day at pH 5.8 (120 min). Overall, optimizing pH and reaction time, are key levers to improve the environmental profile of AOP employing Cu/Fe-PILC catalysts. Full article

This study examines the impacts of climate change on fisheries and aquaculture in Bangladesh, one of the most climate-vulnerable countries in the world. The fisheries and aquaculture sectors contribute significantly to the national GDP and support the livelihoods of 12% of the total population. Using a Critical Literature Review (CLR) approach, peer-reviewed articles, government reports, and official datasets published between 2006 and 2025 were reviewed across databases such as Scopus, Web of Science, FAO, and the Bangladesh Department of Fisheries (DoF). The analysis identifies major climate drivers, including rising temperature, erratic rainfall, salinity intrusion, sea-level rise, floods, droughts, cyclones, and extreme events, and reviews their differentiated impacts on key components of the sector: inland capture fisheries, marine fisheries, and aquaculture systems. For inland capture fisheries, the review highlights habitat degradation, biodiversity loss, and disrupted fish migration and breeding cycles. In aquaculture, particularly in coastal systems, this study reviews the challenges posed by disease outbreaks, water quality deterioration, and disruptions in seed supply, affecting species such as carp, tilapia, pangasius, and shrimp. Coastal aquaculture is also particularly vulnerable to cyclones, tidal surges, and saline water intrusion, with documented economic losses from events such as Cyclones Yaas, Bulbul, Amphan, and Remal. The study synthesizes key findings related to climate-resilient aquaculture practices, monitoring frameworks, ecosystem-based approaches, and community-based adaptation strategies. It underscores the need for targeted interventions, especially in coastal areas facing increasing salinity levels and frequent storms. This study calls for collective action through policy interventions, research and development, and the promotion of climate-smart technologies to enhance resilience and sustain fisheries and aquaculture in the context of a rapidly changing climate. Full article

Blue fescue (Festuca glauca) is a widely used ornamental grass worldwide. Drought is an important limiting factor for the growth and development of blue fescue; therefore, cultivating new strains of blue fescue with a strong drought tolerance is of great significance for its production practice. To investigate the drought tolerance mechanism of ds-1, this study subjected both ds-1 and “Festina” to a natural drought treatment and measured their physiological and biochemical indicators. A transcriptomic analysis was also conducted to explore the underlying molecular mechanisms. The results showed that, after the drought treatment, the relative water content (RWC), water use efficiency (WUE), and photosynthetic rate (Pn) of ds-1 leaves were significantly higher than those of “Festina”; in addition, the contents of H₂O₂ and O₂⁻, the relative electrical conductivity (REC), the malondialdehyde (MDA) content, the gas conductance (Gs), and the transpiration rate (Tr) were significantly lower than those of “Festina”. The peroxidase (POD) activity of ds-1 was significantly higher than that of “Festina”, while the superoxide dismutase (SOD) activity of ds-1 was significantly lower than that of “Festina”. The transcriptome data analysis showed that there were a total of 9475 differentially expressed genes (DEGs) between ds-1 and “Festina”. A Venn plot analysis showed 692 DEGs between ds-1—8d vs. “Festina”—8d and ds-1—16d vs. “Festina”—16d. A KEGG enrichment analysis showed that these 692 genes were mainly enriched in 86 pathways, including those related to the photosynthesis antenna protein, plant hormone signal transduction, MAPK signaling, starch and sucrose metabolism, and arginine and proline metabolism. Further screening identified genes that may be associated with drought stress, including PYL, PP2C, SnRK2, ABF, BRI1, JAZ, MYC2, Lhc, and MPK6. The qRT-PCR results indicated that the expression trends of the DEGs were consistent with the transcriptome sequencing results. Our research results can provide a basis for exploring candidate genes for drought tolerance in blue fescue. In addition, our research results provide valuable genetic resources for the development of drought-resistant ornamental grass varieties, which can help reduce water consumption in cities and decrease labor and capital investment. Full article

As an important ecosystem service, water conservation is influenced by land use related to human activities. In this study, we first evaluated spatial and temporal changes in water conservation in Baicheng City, western Jilin Province, from 2000 to 2020. Then, we identified three different scenarios: the natural development scenario (NDS), cropland protection scenario (CPS), and ecological protection scenario (EPS). We coupled the Patch-generating Land Use Simulation (PLUS) and Integrated Valuation of Ecosystem Services and Trade-offs (InVEST) models to predict the distribution of land use types and water conservation in Baicheng City under these scenarios for 2030. The results showed the following: (1) The average water conservation in Baicheng City from 2000 to 2020 was 7.08 mm. (2) Areas with higher water conservation were distributed in the northwest and northeast, while lower water conservation areas were distributed in the central and southwest of Baicheng City. (3) The simulation results of the future pattern of land use show an increasing water conservation trend in all three scenarios. Compared with the other two scenarios, the ecological protection scenario is the most suitable option for the current development planning of Baicheng City. Under the ecological protection scenario (EPS), ecological land is strictly protected, the area of agricultural land increases to some extent, and the overall structure of changes in land use becomes more rational. This study provides a reference for land resource allocation and ecosystem conservation. Full article

Increasing attention has been given to the environmental impact of tire-derived chemicals in aquatic systems, but submerged whole tires remain an overlooked source. This study investigates a previously unexplored underwater tire dump in Hjelmås Bay, Alver Municipality (Norway) where a blast mat manufacturer discarded large quantities of tires into the bay in the 1970s. These tires have remained submerged for over 50 years. We conducted an initial site mapping and collected sediment and water samples to assess tire-related pollutants in comparison with control sites. Sediment analysis revealed elevated levels of Zn, Pb, and Cu, particularly near the tire dump center, with Zn being the most abundant. Bis(2-ethylhexyl) phthalate (DEHP) was the dominant phthalate detected in the sediments, though no clear spatial pattern emerged for phthalates. Non-target chemical screening of water samples identified 20 features potentially linked to tire degradation, with N,N′-Diphenylguanidine (DPG) being the most notable. Our study highlights the long-term environmental persistence of several tire-derived chemicals, which has ramifications for both the regulation of tire-derived chemicals and plans for remediation at Hjelmås. Our initial findings warrant the implementation of a comprehensive chemical and ecological baseline monitoring assessment prior to discussions on remediation. Full article

Background: Ultraviolet B (UVB) radiation accelerates skin aging by inducing oxidative stress, collagen degradation, and cellular senescence. Although Petasites japonicus is known for its antioxidant properties, its anti-photoaging potential remains underexplored. This research explored the protective properties of a hot water extract from P. japonicus leaves (KP-1) against photoaging caused by UVB exposure. Methods: Hairless mice were exposed to UVB three times per week and orally administered KP-1 for 13 weeks. Wrinkle formation, epidermal thickness, skin hydration, and collagen content were assessed. Protein expression related to MAPK/AP-1, TGF-β/Smad2/3, and p53/p21 pathways was analyzed by Western blotting. Results: KP-1 significantly reduced UVB-induced wrinkle area, epidermal and dermal thickening, and transepidermal water loss while restoring collagen density and skin hydration. KP-1 inhibited MMP-1 expression, enhanced COL1A1 levels, suppressed MAPK/AP-1 activation, and activated TGF-β/Smad2/3 signaling. It also balanced p53/p21 expression and restored cyclin D1 and CDK4 levels, thereby preventing UVB-induced senescence. Conclusions: The findings of this research revealed that KP-1 can serve as a promising natural substance for safeguarding the skin from damage and aging caused by UVB exposure. Full article

Danube Delta (DD), an ecologically vulnerable site, together with fish populations, which are significant food resources, are largely exposed to heavy metal contamination. This study was developed in the Babina–Cernovca sector of DD in September 2023. Zinc (Zn), and iron (Fe) were identified in water, while copper (Cu), iron (Fe), and manganese (Mn) were in sediments (mud). Proximate composition of the muscle tissues of eight fish species identified in the area was assessed. The muscle was also tested to identify heavy metals contamination. The contamination degree was assessed using bioaccumulation and bioconcentrations factors. The relation between nutritional parameters and metals was tested using bivariate and multivariate analyses. Samples were analyzed by specific laboratory tests, and data were processed using ANOVA, Spearman correlation, Principal Component Analysis (PCA), and hierarchical clustering. S. erythrophthalmus, C. gibelio, and A. alburnus have the highest metal bioaccumulation capacity, exhibiting species-specific accumulation patterns. PCA and clustering analysis reflect the influence of species and environmental factors on heavy metal accumulation in fish tissue. The study integrates the heavy metals content with nutritional parameters in fish muscular tissue, using bivariate and multivariate analysis for assessing fish vulnerability to heavy metals exposure in the Danube River. Full article

Background: Parkinson’s disease (PD) is a neurodegenerative disorder for which no curative therapies currently exist. Experimental models employing 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) reproduce PD features such as striatal dopaminergic dysfunction and motor deficits. Various MPTP dosing regimens are used to screen drug candidates for PD, but their validity is limited because of the predominant use of young male animals. Sex bias is another issue that is underrepresented in PD research, since females are more susceptible to this pathology. Here, we studied the model of bolus administration of MPTP (30 mg/kg) in aged female mice and assessed its sensitivity to the antioxidants fullerene C₆₀ and fullerenol C₆₀(OH)₂₄, given that oxidative stress is a key contributor to PD. Methods: 12-month-old female C57BL/6 mice received fullerene (0.1 mg/kg/day, via diet) or fullerenol (0.15 mg/kg/day, via drinking water). On day 10, mice were injected with MPTP. We studied tremor, piloerection, and behavior in the pole test, rotarod, pole test, and open field. High-performance liquid chromatography (HPLC) was employed to study dopaminergic neurotransmission, and the expression levels of its molecular regulators and nitric oxide synthase (NOS)-related targets were investigated using RT-PCR in the striatum and cortex. Results: MPTP-challenged mice displayed profound impairment in markers of dopaminergic neurotransmission and cellular distress, and showed disrupted motor behavior and vegetative functions. Antioxidant-treated animals that received a bolus injection of MPTP demonstrated partial preservation of tremor response, dopaminergic parameters, and iNOS and nNOS gene expression, although motor performance in the pole test was only modestly improved. Fullerenol appeared more effective in decreasing MPTP-induced neurochemical changes. Conclusions: The applied MPTP model showed its validity in mimicking PD features and was sensitive to low doses of antioxidants, suggesting its usefulness for screening drugs that target oxidative and nitrosative stress. The neuroprotective effects of fullerene-based compounds suggest their potential utility in the treatment of PD. Full article

Armed conflicts continue to severely impact human populations and essential infrastructure, particularly water supply systems. This study examines the Yechilla area, a high-intensity battle corridor during the Tigray (between 12°15′26″ 14°57′49″ N latitude; and 36°20′57″–39°58′54″ E longitude) war (2020–2022). Using Cochran’s formula, a representative sample of 89 water schemes was selected for onsite assessment. Additional data on damages to water offices, personnel, equipment, and related infrastructure were gathered through face-to-face interviews with local officials and water professionals, onsite visits, and reviews of governmental and non-governmental archives, and previous studies. The findings reveal that 48.3% of water schemes in the study area are non-functional (does not deliver water), which is a significant increase from pre-war non-functionality rates of approximately 7.1% regionally and 21.1% nationally. Despite the Pretoria peace agreement, non-functionality levels remain critically high two years after conflict. Damage includes partial impairments, lack of technical and spare part support, complete destruction, and looting of water scheme components. The widespread destruction of civilian water infrastructure during the Tigray conflict underscores the insufficiency of existing international legal frameworks, such as the International Humanitarian Law and International Water Law, which are inadequately protecting civilians and their property. Understanding the broader consequences of armed conflicts requires examining the indirect effects and the complex interactions within and between social, economic, and environmental systems. These interconnected impacts are essential to fully grasp how conflict affects livelihoods and human security on a wider scale. Full article

The microbial community in a recirculating aquaculture system (RAS) is pivotal in fish health, contributing significantly to the productive performance during the growing-out phase. Classical and molecular methods using PCR for species-specific amplifications have traditionally been used for bacterial community surveillance. Unfortunately, these approaches mask the real bacterial diversity and abundance, population dynamics, and prevalence of pathogenic bacteria. In this study, we explored the use of Oxford Nanopore Technology to characterize the microbiota and functional metagenomics in a commercial freshwater RAS. Intestine samples from Atlantic salmon (Salmo salar (85 ± 5.7 g)) and water samples from the inlet/outlet water, settling tank, and biofilters were collected. The full-length 16S rRNA gene was sequenced to reconstruct the microbial community, and bioinformatic tools were applied to estimate the functional potential in the RAS and fish microbiota. The analysis showed that bacteria involved in denitrification processes were found in water samples, as well as metabolic pathways related to hydrogen sulfide metabolism. Observations suggested that fish classified as sick exhibited decreased microbial diversity compared with fish without clinical symptomatology (p < 0.05). Proteobacteria were predominant in ill fish, and pathogens of the genera Aeromonas, Aliivibrio, and Vibrio were detected in all intestinal samples. Notably, Aliivibrio wodanis was detected in fish showing abnormal clinical conditions. Healthy salmon showed higher contributions of pathways related to amino acid metabolism and short-chain fatty acid fermentation (p < 0.05), which may indicate more favorable fish conditions. These findings suggest the utility of nanopore sequencing methods in assessing the microbial community in RASs for salmon aquaculture. Full article