Search Results (128)

The valorization of wine lees, a major by-product of winemaking, is gaining attention as part of broader initiatives to promote circular economy and sustainable resource use in the agri-food sector. This study assessed ultrasound (US), pulsed electric fields (PEF), and their combination (PEF + US) as non-thermal technologies for promoting yeast autolysis and recovering intracellular proteins from wine lees. All treatments effectively reduced yeast viability, with populations decreasing from ~7.0 to ~4.7 log CFU/mL within 5 min. PEF alone achieved this microbial inactivation with a low energy input of 25–100 kJ/kg. In contrast, US yielded the highest protein release (~5700 μg/mL after 20 min), although it required a substantial energy input (~19,800 kJ/kg). The combined PEF + US method provided comparable protein yields (~5400 μg/mL) while reducing energy consumption by more than 50%. These results demonstrate that PEF is the optimal method for low-energy microbial inactivation, whereas US and PEF + US are more effective for protein recovery. The synergy of the combined approach offers a balanced and scalable solution for sustainable bioprocessing, reinforcing the potential of hybrid technologies in the green extraction of wine industry by-products and their integration into circular bioeconomy strategies. Full article

To reveal the characteristic physicochemical indicators of wines of different quality grades and explore their feasibility as auxiliary indicators for grading, 23 wines from the Manas subregion of Xinjiang were used as test materials. Sensory evaluation, colour difference analysis, and electronic tongue technology were employed, combined with nontargeted metabolomics and quantitative analysis, to analyze differences in phenolic compounds, colour parameters, and taste characteristics among wines of different grades. Finally, a quality evaluation model for Cabernet Sauvignon wine was constructed using partial least squares regression (PLSR). The results revealed significant differences in the L* values, a* values, and C*ab values among wines of different grades. Grade A wines presented lower L* values, higher a* values, and higher C*ab values, indicating lower brightness, deeper red tones, and higher saturation. Taste characteristic differences were primarily manifested in Grade A wines, which have higher acidity, astringency, bitterness, and richness but exhibit lower bitterness aftertaste and astringency aftertaste. The results of the quantitative analysis and correlation analysis indicate that the differences in sensory characteristics among different grades of wine stem from variations in their polyphenolic compound contents. The higher anthocyanin content in Grade A wine is associated with higher a* values; higher flavonoid content is closely related to higher astringency and bitterness values; and lower flavanol content is associated with lower bitterness aftertaste and astringency aftertaste values. The PLSR model results indicate that when sensory characteristic parameters and phenolic compound content are used as predictor variables (X) and grade is used as the response variable (Y), the PLSR model has a calibration set R² = 0.97 and a validation set R² = 0.92, the calibration set RMSE is 0.13, and the validation set RMSE is 0.25. The model demonstrates good fitting performance, establishing an objective method for evaluating wine quality that avoids evaluation errors caused by the subjective factors of winemakers and tasters. This study is the first to conduct a comprehensive evaluation of the sensory characteristic and chemical components of three grades of wine, providing data support and theoretical references for the improvement of wine quality evaluation systems. Full article

Although beer fermentation has traditionally been carried out with Saccharomyces, the boom in craft brewing has led to the use of non-conventional yeast species for beer production. This group also includes non-Saccharomyces starters, which are commonly used in winemaking and which have different technological characteristics compared to standard representatives of the Saccharomyces genus. One of the important characteristics of the non-Saccharomyces group is the richer enzyme profile, which leads to the production of beverages with different taste and aroma profiles. The aim of this study was to investigate sweet and hopped wort fermentation with seven strains of active dry non-conventional yeasts of Lachancea spp., Metschnikowia spp., Torulaspora spp. and a mixed culture of Saccharomyces cerevisiae and Torulaspora delbrueckii. One ale and one lager active dry yeast strain were used as control strains. The extract consumption, ethanol production, degree of fermentation, pH drop, as well as the yeast secondary metabolites formed by the yeast (higher alcohols, esters and aldehydes) in sweet and hopped wort were investigated. The results indicated that all of the studied types of non-conventional yeasts have serious potential for use in beer production in order to obtain new beer styles. For the purposes of this study, statistical methods, principle component analysis (PCA) and correlation analysis were used, thus establishing the difference in the fermentation kinetics of the growth in the studied species in sweet and hopped wort. It was found that hopping had a significant influence on the fermentation kinetics of some of the species, which was probably due to the inhibitory effect of the iso-alpha-acids of hops. Directions for future research with the studied yeast species in beer production are presented. Full article

Climate change has a significant negative impact on agriculture and food production. This trend requires technological development and the adaptation of new technologies in both the grapevine production and winemaking sectors. High temperatures and heat accumulation during the growing season result in faster ripening and a higher sugar content, leading to a higher alcohol content during fermentation. The negative consequences are an imbalanced wine character and consumer reluctance, as lower alcoholic beverages are now in high demand. Over the last decade, several methods have been developed to handle this impact and reduce the alcohol content of wines. In this study, we used the MASTERMIND^® REMOVE membrane-based dealcoholization system to reduce the alcohol concentration in of Pinot gris wines from 12.02% v/v to 10.69% v/v and to investigate the effect on analytical parameters in three steps (0.5%, 1%, and 1.5% reductions) along the treatment. To evaluate the impact of the partial alcohol reduction and identify correlations between the wine chemical parameters, data were analyzed with ANOVA, PCA, multivariate linear regression and cluster analysis. The results showed that except for the extract, sugar content and proline content, the treatment had a significant effect on the chemical parameters. Both free and total SO₂ levels were significantly reduced as well as volatile acid, glycerol and succinic acid levels. It must be highlighted that some parameters were not differing significantly between the untreated and the final wine, while the change was statistically verified in the intermediate steps of the partial alcohol reduction. This was the case for example for n-Propanol, i-Amylalcohol, Acetaldehyde, and Ethyl acetate. The multivariate linear regression model explained 18.84% of the total variance, indicating a modest but meaningful relationship between the alcohol content and the investigated analytical parameters. Our results showed that even if the applied instrument significantly modified some of the wine chemical parameters, those changes would not influence significantly the wine sensory attributes. Full article

Yeast genetic improvement is entering a transformative phase, driven by the integration of artificial intelligence (AI), big data analytics, and synthetic microbial communities with conventional methods such as sexual breeding and random mutagenesis. These advancements have substantially expanded the potential for innovative re-engineering of yeast, ranging from single-strain cultures to complex polymicrobial consortia. This review compares traditional genetic manipulation techniques with cutting-edge approaches, highlighting recent breakthroughs in their application to beer and wine fermentation. Among the innovative strategies, adaptive laboratory evolution (ALE) stands out as a non-GMO method capable of rewiring complex fitness-related phenotypes through iterative selection. In contrast, GMO-based synthetic biology approaches, including the most recent developments in CRISPR/Cas9 technologies, enable efficient and scalable genome editing, including multiplexed modifications. These innovations are expected to accelerate product development, reduce costs, and enhance the environmental sustainability of brewing and winemaking. However, despite their technological potential, GMO-based strategies continue to face significant regulatory and market challenges, which limit their widespread adoption in the fermentation industry. Full article

The characteristics of the variety from which the wine is made, the geographical area of production, the year of production, and the technology of winemaking are the parameters with the most influence on the total content of polyphenols, the polyphenolic profile, and the antioxidant activity of wine. For this reason, a polyphenolic screen can help establish the authenticity of wines. For this study, 50 samples of white and red wine from different wine areas were collected. For these samples, a qualitative and quantitative analysis was carried out on the polyphenolic profile. The polyphenolic profiles of the studied wines were determined using UHPLC-ESI-MS/MS (mass spectrometry with tandem ionization with high-performance liquid chromatography). Among the non-flavonoid acids, gallic acids, p-coumarnic, and syringic acids in red wines showed higher concentrations in all samples, while resveratrol was present in concentrations from 0.605 to 12.38 mg/L in red wines, and white wines ranged from 0.07 to 0.35 mg/L. For flavonoids, -catechin (0.187 m/L–130.98 mg/L in red wines and 0.04–4.45 mg/L) and (-)-epi-catechin showed the highest concentrations, reaching up to 29.78 mg/L in red wines. Full article

Significant advances in winemaking equipment and processes, as well as a deeper understanding of the role of yeast, have significantly improved wine quality throughout history. This paper examines critical aspects related to the use of commercial wine yeast starter cultures in the fermentation of grape and fruit wines, with a focus on berry wines and blackberry wine, which is the most predominant berry wine in Croatia. While the production of grape wines remains the most significant, fruit wines are gaining importance due to their composition, which contains a variety of bioactive compounds. Although spontaneous fermentation is still preferred by some winemakers, controlled or inoculated fermentation, based on the use of wine yeast starter cultures, is predominantly employed in modern winemaking. The selection of suitable yeast strains for grape wines is easier than for fruit wines, as the broader availability of commercial yeasts for grape wines contrasts with the limited selection offered for fruit wine production due to the smaller fruit wine market. The selection of Saccharomyces and, more recently, non-Saccharomyces yeast strains with desirable characteristics are crucial for the production of high-quality wines. Selection criteria for wine yeasts have evolved to meet modern consumer preferences and focus on technological properties, secondary flavor development and health effects. Full article

Winemakers are increasingly adopting partial or total dealcoholisation of wines and alcoholic beverages in response to market trends and the impact of climate change on wine alcohol content. In this study, the patented very low-temperature vacuum wine distillation technology known as GoLo was used to dealcoholise various types of red, white, and rosé wines from different Spanish designations of origin (DOs) in order to examine changes in oenological parameters as pH, sulphites, total acidity, volatile acidity, and sugars and the perceptible differences among a set of wines through sensory analysis and the chemical analysis related, such as turbidity, total phenolic content, and a profile of volatile compounds. The results indicate that there is an increase in the overall polyphenol content in the range of 8 to 12%, turbidity increased in the range of 13 to 70%, and sulphites decreasing in the range of 20 to 40%. The aroma profile also reveals that the most volatile and less soluble compounds—such as esters (reduction between 60% and 96%), terpenes (reduction between 45% and 80%), and aldehydes (reduction between 86% and 95%)—are lost in varying percentages during the dealcoholisation process, depending on the type of wine. Meanwhile, other more soluble compounds like phenols and acids undergo transformations during GoLo’s dealcoholisation process, leading to an increase in their concentrations up to more than 90% in the case of phenols in white wines or 35% for acids in red wines. Full article

Sparkling wine production has changed over time due to ongoing technological developments and market adjustments. This study provides an overview of the historical context, the characteristics of raw materials and base wine, modern technologies and current trends in the sparkling wine market. Important scientific advances with potential for wide-scale applicability are highlighted, such as the investigation of unconventional grape types, the progress of winemaking methods and the effects of compositional changes on quality and sensory characteristics. In addition, the nutritional effects of bioactive components in wine are discussed. Market trends indicate a growing global demand for sparkling wines over time, driven by changing consumer preferences and the diversification of wine-producing areas. Future prospects focus on sustainability, low-alcohol alternatives and the integration of emerging technologies. Combining tradition with innovation, the sparkling wine industry continues to expand, offering new opportunities to both producers and consumers. Research on the quality of sparkling wines in the context of climate change and evolving consumer preferences is still limited and warrants greater attention. Full article

Argentina ranks worldwide among the top ten wine producers, known for its diverse terroirs and Malbec as its emblematic varietal. Typically, the winemaking process involves alcoholic fermentation, led by yeasts, and malolactic fermentation (MLF), primarily driven by lactic acid bacteria (LAB). Oenococcus oeni and Lactiplantibacillus plantarum are recognised as the best-adapted LAB species for this process. Our previous research focused on a winery located in the southwest of Buenos Aires Province, a scarcely studied re-emerging region of Argentina, which showed a low relative abundance of LAB and incomplete MLF in various vintages. The current study involved the isolation, identification, typing, and use of native strains from the above-mentioned region to formulate a malolactic fermentation starter (MLFS) and to evaluate the strains’ malolactic performance at pilot-scale, implantation capacity and impact on wine aromatic profiles using HS-SPME-GC-FID/MS. Two selected autochthonous strains (Lpb. plantarum UNQLp1001 and a O. oeni UNQOe1101) from the re-emerging region successfully implanted in Malbec wine, achieving faster and more efficient MLF compared to spontaneous MLF. Moreover, the MLFS seems to have influenced the aromatic profile, reducing relative concentrations of alcohols, contributing to the decrease in the bitter and herbaceous notes, and increasing some esters (ethyl acetate, 2-phenethyl acetate, ethyl octanoate), that could enhance floral and fruity, notes. Expanding the availability of candidate strains to formulate native MLFS is a crucial technological tool for the wine industry. Thus, we propose the use of Lpb. plantarum UNQLp1001 and O. oeni UNQOe1101 as potential MLFS in Malbec wines from somewhat similar wine-producing regions. Additionally, the local winery can access a cost-effective MLFS with native LAB strains, enabling a more controlled MLF that preserves regional typicity. Moreover, these strains could enable technology transfer, potentially becoming the first malolactic starters in the region. Full article

Yeast derivatives are additives commonly used in winemaking for different purposes. Their manufacturing process is not well standardized, being mostly based on thermal inactivation and enzyme-induced lysis; furthermore, the main strain currently authorized for their production belongs to Saccharomyces spp. In this study, Saccharomyces cerevisiae and Torulaspora delbrueckii were used as starting microorganisms, whereas ultrasounds and high hydrostatic pressure were performed to induce autolysis, with the aim to evaluate the possibility to use different strains and emerging technologies as alternatives to the traditional methods to produce yeast derivatives. The chemical composition of the products obtained as well as the volatile profile of wines aged on yeast derivatives were mostly affected by the treatments performed during the manufacturing process. T. delbrueckii showed a good aptitude as starting microorganism for producing derivatives, whereas emerging, non-thermal technologies could replace the traditional methods for inducing autolysis, allowing to obtain products with enhanced content of polysaccharides (up to 178 mg/g) and antioxidant compounds (up to 9 µmol/g), and with low odor impact. The possibility to manage the chemical composition of yeast derivatives for specific winemaking purposes may thus be possible, by using specific starting microorganism and by applying the most suitable treatment to induce autolysis. Full article

The community of epibiotic yeasts significantly influences the quality of Docynia delavayi (Franch.) Schneid. wine. The yeast diversity in four different Docynia delavayi (Franch.) Schneid. wines during the brewing stage was investigated using pure culture methods and high-throughput sequencing technology. A total of 229,381,292 sequencing bases were generated, yielding 323,820 valid sequences with an average length of 708 nt and identifying 93 operational taxonomic units (OTUs) from naturally fermented samples of Docynia delavayi (Franch.) Schneid. wine for classification purposes. At the early fermentation stage, Hanseniaspora sp. was identified as the dominant species, whereas at the late fermentation stage, Hanseniaspora sp., Saccharomyces sp., and Candida californica became predominant. From these samples, a total of 109 yeast strains were isolated from Docynia delavayi (Franch.) Schneid. wine. Three specific strains—LZX-76, LZX-89, and LZX-104—were further selected based on their growth characteristics along with hydrogen sulfide production, ester production, ethanol production, and tolerance levels. Through morphological examination and molecular biology techniques, these strains were identified as Pichia fermentans and Hanseniaspora spp. Additionally, a total of 29 volatile compounds were detected through simulated fermentation processes; these included 12 esters, 6 alcohols, 2 acids, 4 aldehydes, and 5 other compounds. When compared to commercial yeasts used as starters in winemaking processes, it was observed that utilizing yeast strains LZX-76, LZX-89, and LZX-104 resulted in an increased number of volatile compounds, which enhanced the aromatic profile characteristics of Docynia delavayi (Franch.) Schneid. wine by making its aroma richer and more complex. The findings from this study hold significant potential value for both the production practices and research endeavors related to Docynia delavayi (Franch.) Schneid. wine. Full article

Viticulture, the science of growing, cultivating, and harvesting grapes, and enology, the art and science of making wine, are rapidly evolving through innovative approaches aimed at improving the quality and efficiency of grape and wine production. This review explores the emerging use of nanoparticles, in particular gold, silver, and magnetic nanoparticles, to improve the quality, safety, and sustainability of both grape growing and winemaking processes. The unique properties of these nanoparticles, such as their small size, high surface area, and distinct chemical properties, enable them to address key challenges within the industry. In viticulture, nanoparticles have shown potential in protecting vines from pathogens, optimizing grape yield, and improving quality. In enology, nanoparticles are making a significant contribution to microbial control, reducing spoilage and refining wine analysis techniques, leading to improved product quality and safety. This review also highlights the synergy between different types of nanoparticles and their diverse applications, from microbial control in wine production to their use in innovative packaging solutions. In addition, nanoparticles have the potential to reduce dependence on agrochemicals and improve the sustainability of wine production, which is a promising avenue for future research. However, the integration of nanoparticles in viticulture and enology also poses regulatory and safety challenges, including the potential for nanoparticles to leach into wine products. Further research and regulatory advances are essential to ensure the safe and effective use of these technologies in winemaking. Overall, nanoparticles offer significant benefits to the wine industry, driving improvements in efficiency, sustainability, and quality. Full article

An abundant byproduct of the Uruguayan winemaking industry, Tannat grape pomace (TGP), has a unique profile of phenolic compounds, making it a great candidate as an ingredient in the formulation of healthy and sustainable foods. The addition of TGP and sweetener to a muffin formulation may represent a challenge regarding technological properties. In this work, the objective was to develop potential functional muffins with the nutritional claims of “source of/high in fiber” and “no added sugars”, by incorporating TGP as a source of fiber and bioactive compounds and stevia as a sweetener. For this purpose, a factorial experimental design with central points was assessed by varying TGP and sweetener contents. Color was measured in the muffins by a CieLab system, and texture parameters (hardness, elasticity, cohesiveness, gumminess, and chewiness) were obtained by a texture analyzer. The antioxidant capacity was also assessed by total phenol content (TPC by Folin reaction), ABTS, and ORAC-FL methods. Regarding color parameters, the main results showed lower L values for the muffins with higher TGP content (34.4–35.9, p < 0.05). As for texture parameters, lower hardness values (3170–3655 N) were displayed by muffins with higher TGP content (p < 0.05). Elasticity showed no significant differences between the samples (0.773–0.873), with the exception of one of the formulations with higher TGP content. Cohesiveness values ranged between 0.210 and 0.374. Gumminess and chewiness values were lower for the muffin formulation with higher TGP and stevia contents (694 ± 202 N and 538 ± 182 N). Moreover, antioxidant capacity showed increased TPC values for muffins with higher TGP content when compared to the control muffin (without TGP). Similarly, ABTS and ORAC-FL values were higher for the muffins with higher TGP content (15.26–15.59 and 23.92–25.56 µmol Trolox equivalent (TE)/g samples, respectively). In conclusion, TGP muffins represent a sustainable food with adequate technological properties and enhanced nutritional properties, presenting potential to promote health. Full article

The utilization of membrane technologies in winemaking has revolutionized various stages of production, offering precise and efficient alternatives to traditional methods. Membranes, characterized by their selective permeability, play a pivotal role in enhancing wine quality across multiple processes. In clarification, microfiltration and ultrafiltration membranes, such as ceramic or polymeric membranes (e.g., polyethersulfone or PVDF), effectively remove suspended solids and colloids, resulting in a clearer wine without the need for chemical agents. During stabilization, membranes such as nanofiltration and reverse osmosis membranes, often made from polyamide composite materials, enable the selective removal of proteins, polysaccharides, and microorganisms, thereby improving the wine’s stability and extending its shelf life. Additionally, in dealcoholization, membranes like reverse osmosis and pervaporation membranes, typically constructed from polydimethylsiloxane (PDMS) or other specialized polymers, facilitate the selective removal of ethanol while preserving the wine’s flavor and aroma profile, addressing the increasing consumer demand for low-alcohol and alcohol-free wines. This article provides a comprehensive analysis of the advancements and applications of membrane technologies in winemaking. Full article