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Int. J. Mol. Sci. 2012, 13(4), 5230-5241; doi:10.3390/ijms13045230

Enhanced Production of a Novel Cyclic Hexapeptide Antibiotic (NW-G01) by Streptomyces alboflavus 313 Using Response Surface Methodology

1,2,* , 3
,
1,4,5
and
1,4,5,*
1
State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, China
2
Anhui provincial laboratory of Agro-Food safety, Resources & Environment College, Anhui Agricultural University, Hefei 230036, China
3
School of Life Sciences, Anhui Agricultural University, Hefei 230036, China
4
Shaanxi Province Key Laboratory Research & Development on Botanical Pesticide, Northwest A & F University, Yangling 712100, China
5
Key Laboratory of Plant Protection Resources and Pest Integrated Management, Ministry of Education, College of Plant Protection, Northwest A & F University, Yangling 712100, China
*
Authors to whom correspondence should be addressed.
Received: 6 January 2012 / Revised: 16 April 2012 / Accepted: 17 April 2012 / Published: 24 April 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

NW-G01, produced by Streptomyces alboflavus 313, is a novel cyclic hexapeptide antibiotic with many potential applications, including antimicrobial activity and antitumor agents. This study developed a system for optimizing medium components in order to enhance NW-G01 production. In this study, Plackett-Burman design (PBD) was used to find the key ingredients of medium components, and then response surface methodology (RSM) was implemented to determine their optimal concentrations. The results of PBD revealed that the crucial ingredients related to the production of NW-G01 were (NH4)2SO4, peptone and CaCO3. A prediction model has been built in the experiments of central composite design and response surface methodology, and its validation has been further verified. The optimal medium composition was determined (g/L): corn starch 15, glucose 15, peptone 3.80, (NH4)2SO4 0.06, NaCl 1.5, CaCO3 1.30, MgSO4·7H2O 0.015, K2HPO4·3H2O 0.015, MnCl2·4H2O 0.015, FeSO4·7H2O 0.015, and ZnSO4·7H2O 0.015. Compared with NW-G01 production (5.707 mg/L) in non-optimized fermentation medium, the production of NW-G01 (15.564 mg/L) in optimized fermentation medium had a 2.73-fold increase. View Full-Text
Keywords: NW-G01; medium optimization; Streptomyces alboflavus 313; Plackett-Burman design; response surface methodology NW-G01; medium optimization; Streptomyces alboflavus 313; Plackett-Burman design; response surface methodology
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Guo, Z.; Shen, L.; Ji, Z.; Wu, W. Enhanced Production of a Novel Cyclic Hexapeptide Antibiotic (NW-G01) by Streptomyces alboflavus 313 Using Response Surface Methodology. Int. J. Mol. Sci. 2012, 13, 5230-5241.

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