Next Article in Journal
Combinatorial Analysis of Secretory Immunoglobulin A (sIgA) Expression in Plants
Previous Article in Journal
The Lipid Transfer Protein StarD7: Structure, Function, and Regulation
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 14
Issue 3
10.3390/ijms14036187
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière)

by
Zhong Chen
,
Jia Wang
,
Mei-Xia Ye
,
Hao Li
,
Le-Xiang Ji
,
Ying Li
,
Dong-Qing Cui
,
Jun-Mei Liu
and
Xin-Min An
*
National Engineering Laboratory for Tree Breeding (NDRC), Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants (MOE), the Tree and Ornamental Plant Breeding and Biotechnology Laboratory (SFA), College of Biological Science and Biotechnology, Beijing Forestry University, Qinghua Eastern Road No.35, Haidian District, Beijing 100083, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2013, 14(3), 6187-6204; https://doi.org/10.3390/ijms14036187
Submission received: 21 November 2012 / Revised: 5 February 2013 / Accepted: 6 March 2013 / Published: 18 March 2013
Browse Figures
Versions Notes

Abstract

A novel sequence that functions as a promoter element for moderate constitutive expression of transgenes, designated as the PtMCP promoter, was isolated from the woody perennial Populus tomentosa. The PtMCP promoter was fused to the GUS reporter gene to characterize its expression pattern in different species. In stable Arabidopsis transformants, transcripts of the GUS reporter gene could be detected by RT-PCR in the root, stem, leaf, flower and silique. Further histochemical and fluorometric GUS activity assays demonstrated that the promoter could direct transgene expression in all tissues and organs, including roots, stems, rosette leaves, cauline leaves and flowers of seedlings and maturing plants. Its constitutive expression pattern was similar to that of the CaMV35S promoter, but the level of GUS activity was significantly lower than in CaMV35S promoter::GUS plants. We also characterized the promoter through transient expression in transgenic tobacco and observed similar expression patterns. Histochemical GUS staining and quantitative analysis detected GUS activity in all tissues and organs of tobacco, including roots, stems, leaves, flower buds and flowers, but GUS activity in PtMCP promoter::GUS plants was significantly lower than in CaMV35S promoter::GUS plants. Our results suggested that the PtMCP promoter from poplar is a constitutive promoter with moderate activity and that its function is presumably conserved in different species. Therefore, the PtMCP promoter may provide a practical choice to direct moderate level constitutive expression of transgenes and could be a valuable new tool in plant genetic engineering.
Keywords: constitutive promoter; moderate expression; RT-PCR; GUS; transgenic plants constitutive promoter; moderate expression; RT-PCR; GUS; transgenic plants

Share and Cite

MDPI and ACS Style

Chen, Z.; Wang, J.; Ye, M.-X.; Li, H.; Ji, L.-X.; Li, Y.; Cui, D.-Q.; Liu, J.-M.; An, X.-M. A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière). Int. J. Mol. Sci. 2013, 14, 6187-6204. https://doi.org/10.3390/ijms14036187

AMA Style

Chen Z, Wang J, Ye M-X, Li H, Ji L-X, Li Y, Cui D-Q, Liu J-M, An X-M. A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière). International Journal of Molecular Sciences. 2013; 14(3):6187-6204. https://doi.org/10.3390/ijms14036187

Chicago/Turabian Style

Chen, Zhong, Jia Wang, Mei-Xia Ye, Hao Li, Le-Xiang Ji, Ying Li, Dong-Qing Cui, Jun-Mei Liu, and Xin-Min An. 2013. "A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière)" International Journal of Molecular Sciences 14, no. 3: 6187-6204. https://doi.org/10.3390/ijms14036187

APA Style

Chen, Z., Wang, J., Ye, M.-X., Li, H., Ji, L.-X., Li, Y., Cui, D.-Q., Liu, J.-M., & An, X.-M. (2013). A Novel Moderate Constitutive Promoter Derived from Poplar (Populus tomentosa Carrière). International Journal of Molecular Sciences, 14(3), 6187-6204. https://doi.org/10.3390/ijms14036187

Article Metrics

Back to TopTop