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Sensors 2012, 12(12), 17247-17261; doi:10.3390/s121217247
Article

An Amperometric Immunosensor Based on Multi-Walled Carbon Nanotubes-Thionine-Chitosan Nanocomposite Film for Chlorpyrifos Detection

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School of Agriculture and Food Engineering, Shandong University of Technology, No.12, Zhangzhou Road, Zibo 255049, China
* Author to whom correspondence should be addressed.
Received: 19 October 2012 / Revised: 10 December 2012 / Accepted: 11 December 2012 / Published: 13 December 2012
(This article belongs to the Section Biosensors)
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Abstract

In this work, a novel amperometric immunosensor based on multi-walled carbon nanotubes-thionine-chitosan (MWCNTs-THI-CHIT) nanocomposite film as electrode modified material was developed for the detection of chlorpyrifos residues. The nanocomposite film was dropped onto a glassy carbon electrode (GCE), and then the anti-chlorpyrifos monoclonal antibody was covalently immobilized onto the surface of MWCNTs-THI-CHIT/GCE using the crosslinking agent glutaraldehyde (GA). The modification procedure was characterized by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, a linear relationship between the relative change in peak current of different pulse voltammetry (DPV) and the logarithm of chlorpyrifos solution concentration was obtained in the range from 0.1 to 1.0 × 105 ng/mL with a detection limit of 0.046 ng/mL. The proposed chlorpyrifos immunosensor exhibited high reproducibility, stability, and good selectivity and regeneration, making it a potential alternative tool for ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.
Keywords: multi-walled carbon nanotubes; thionine; chitosan; chlorpyrifos; immunosensor multi-walled carbon nanotubes; thionine; chitosan; chlorpyrifos; immunosensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Sun, X.; Cao, Y.; Gong, Z.; Wang, X.; Zhang, Y.; Gao, J. An Amperometric Immunosensor Based on Multi-Walled Carbon Nanotubes-Thionine-Chitosan Nanocomposite Film for Chlorpyrifos Detection. Sensors 2012, 12, 17247-17261.

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