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Sensors 2012, 12(8), 10487-10499; doi:10.3390/s120810487
Article

Detection of Non-PCR Amplified S. enteritidis Genomic DNA from Food Matrices Using a Gold-Nanoparticle DNA Biosensor: A Proof-of-Concept Study

1
,
2 and 2,*
1 Department of Biology, Whittier College, 13406 E. Philadelphia St., Whittier, CA 90608, USA 2 Department of Biosystems and Agricultural Engineering, 213 Farrall Hall, Michigan State University, East Lansing, MI 48824, USA
* Author to whom correspondence should be addressed.
Received: 4 June 2012 / Revised: 27 July 2012 / Accepted: 31 July 2012 / Published: 2 August 2012
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Abstract

Bacterial pathogens pose an increasing food safety and bioterrorism concern. Current DNA detection methods utilizing sensitive nanotechnology and biosensors have shown excellent detection, but require expensive and time-consuming polymerase chain reaction (PCR) to amplify DNA targets; thus, a faster, more economical method is still essential. In this proof-of-concept study, we investigated the ability of a gold nanoparticle-DNA (AuNP-DNA) biosensor to detect non-PCR amplified genomic Salmonella enterica serovar Enteritidis (S. enteritidis) DNA, from pure or mixed bacterial culture and spiked liquid matrices. Non-PCR amplified DNA was hybridized into sandwich-like structures (magnetic nanoparticles/DNA/AuNPs) and analyzed through detection of gold voltammetric peaks using differential pulse voltammetry. Our preliminary data indicate that non-PCR amplified genomic DNA can be detected at a concentration as low as 100 ng/mL from bacterial cultures and spiked liquid matrices, similar to reported PCR amplified detection levels. These findings also suggest that AuNP-DNA biosensors are a first step towards a viable detection method of bacterial pathogens, in particular, for resource-limited settings, such as field-based or economically limited conditions. Future efforts will focus on further optimization of the DNA extraction method and AuNP-biosensors, to increase sensitivity at lower DNA target concentrations from food matrices comparable to PCR amplified DNA detection strategies.
Keywords: biosensor; gold-nanoparticles; DNA probe; genomic DNA; food matrix biosensor; gold-nanoparticles; DNA probe; genomic DNA; food matrix
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Vetrone, S.A.; Huarng, M.C.; Alocilja, E.C. Detection of Non-PCR Amplified S. enteritidis Genomic DNA from Food Matrices Using a Gold-Nanoparticle DNA Biosensor: A Proof-of-Concept Study. Sensors 2012, 12, 10487-10499.

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