Next Article in Journal
A Four-Quadrant PVDF Transducer for Surface Acoustic Wave Detection
Next Article in Special Issue
A Fast and Sensitive Quantitative Lateral Flow Immunoassay for Cry1Ab Based on a Novel Signal Amplification Conjugate
Previous Article in Journal
Cognitive LF-Ant: A Novel Protocol for Healthcare Wireless Sensor Networks
Article Menu

Export Article

Open AccessArticle
Sensors 2012, 12(8), 10487-10499; doi:10.3390/s120810487

Detection of Non-PCR Amplified S. enteritidis Genomic DNA from Food Matrices Using a Gold-Nanoparticle DNA Biosensor: A Proof-of-Concept Study

Department of Biology, Whittier College, 13406 E. Philadelphia St., Whittier, CA 90608, USA
Department of Biosystems and Agricultural Engineering, 213 Farrall Hall, Michigan State University, East Lansing, MI 48824, USA
Author to whom correspondence should be addressed.
Received: 4 June 2012 / Revised: 27 July 2012 / Accepted: 31 July 2012 / Published: 2 August 2012
View Full-Text   |   Download PDF [311 KB, uploaded 21 June 2014]   |  


Bacterial pathogens pose an increasing food safety and bioterrorism concern. Current DNA detection methods utilizing sensitive nanotechnology and biosensors have shown excellent detection, but require expensive and time-consuming polymerase chain reaction (PCR) to amplify DNA targets; thus, a faster, more economical method is still essential. In this proof-of-concept study, we investigated the ability of a gold nanoparticle-DNA (AuNP-DNA) biosensor to detect non-PCR amplified genomic Salmonella enterica serovar Enteritidis (S. enteritidis) DNA, from pure or mixed bacterial culture and spiked liquid matrices. Non-PCR amplified DNA was hybridized into sandwich-like structures (magnetic nanoparticles/DNA/AuNPs) and analyzed through detection of gold voltammetric peaks using differential pulse voltammetry. Our preliminary data indicate that non-PCR amplified genomic DNA can be detected at a concentration as low as 100 ng/mL from bacterial cultures and spiked liquid matrices, similar to reported PCR amplified detection levels. These findings also suggest that AuNP-DNA biosensors are a first step towards a viable detection method of bacterial pathogens, in particular, for resource-limited settings, such as field-based or economically limited conditions. Future efforts will focus on further optimization of the DNA extraction method and AuNP-biosensors, to increase sensitivity at lower DNA target concentrations from food matrices comparable to PCR amplified DNA detection strategies. View Full-Text
Keywords: biosensor; gold-nanoparticles; DNA probe; genomic DNA; food matrix biosensor; gold-nanoparticles; DNA probe; genomic DNA; food matrix

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Vetrone, S.A.; Huarng, M.C.; Alocilja, E.C. Detection of Non-PCR Amplified S. enteritidis Genomic DNA from Food Matrices Using a Gold-Nanoparticle DNA Biosensor: A Proof-of-Concept Study. Sensors 2012, 12, 10487-10499.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top