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Sensors 2013, 13(5), 6865-6881; doi:10.3390/s130506865

Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes

The Co-Construction Laboratory of Microbial Molecular Biology of Province Department and Ministry of Science and Technology, College of Life Sciences, Hunan Normal University, Changsha 410081, China
Author to whom correspondence should be addressed.
Received: 3 April 2013 / Revised: 22 April 2013 / Accepted: 17 May 2013 / Published: 22 May 2013
(This article belongs to the Special Issue Nanotube and Nanowire Sensors)
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In this paper, a panel of single-stranded DNA aptamers with high affinity and specificity against Salmonella Paratyphi A was selected from an enriched oligonucleotide pool by a whole-cell-Systematic Evolution of Ligands by Exponential Enrichment (SELEX) procedure, during which four other Salmonella serovars were used as counter-selection targets. It was determined through a fluorescence assay that the selected aptamers had high binding ability and specificity to this pathogen. The dissociation constant of these aptamers were up to nanomolar range, and aptamer Apt22 with the lowest Kd (47 ± 3 nM) was used in cell imaging experiments. To detect this bacteria with high specificity and cost-efficiently, a novel useful detection method was also constructed based on the noncovalent self-assembly of single-walled carbon nanotubes (SWNTs) and DNAzyme-labeled aptamer detection probes. The amounts of target bacteria could be quantified by exploiting chemoluminescence intensity changes at 420 nm and the detection limit of the method was 103 cfu/mL. This study demonstrated the applicability of Salmonella specific aptamers and their potential for use in the detection of Salmonella in food, clinical and environmental samples. View Full-Text
Keywords: Salmonella Paratyphi A; aptamer; single-walled carbon nanotubes; detection Salmonella Paratyphi A; aptamer; single-walled carbon nanotubes; detection

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yang, M.; Peng, Z.; Ning, Y.; Chen, Y.; Zhou, Q.; Deng, L. Highly Specific and Cost-Efficient Detection of Salmonella Paratyphi A Combining Aptamers with Single-Walled Carbon Nanotubes. Sensors 2013, 13, 6865-6881.

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