Next Article in Journal
Ontology-Driven Monitoring of Patient’s Vital Signs Enabling Personalized Medical Detection and Alert
Next Article in Special Issue
Detection of Virgin Olive Oil Adulteration Using Low Field Unilateral NMR
Previous Article in Journal
In-Line Measurement of Water Content in Ethanol Using a PVA-Coated Quartz Crystal Microbalance
Previous Article in Special Issue
Calibrationless Parallel Magnetic Resonance Imaging: A Joint Sparsity Model
Article Menu

Export Article

Open AccessReview
Sensors 2014, 14(1), 1576-1597; doi:10.3390/s140101576

Hyperpolarized NMR Probes for Biological Assays

1
Carlsberg Laboratory, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark
2
Albeda Research, Gamle Carlsberg Vej 10, 1799 Copenhagen V, Denmark
*
Authors to whom correspondence should be addressed.
Received: 28 November 2013 / Revised: 20 December 2013 / Accepted: 7 January 2014 / Published: 16 January 2014
(This article belongs to the Special Issue Magnetic Resonance Sensors)
View Full-Text   |   Download PDF [3202 KB, uploaded 21 June 2014]   |  

Abstract

During the last decade, the development of nuclear spin polarization enhanced (hyperpolarized) molecular probes has opened up new opportunities for studying the inner workings of living cells in real time. The hyperpolarized probes are produced ex situ, introduced into biological systems and detected with high sensitivity and contrast against background signals using high resolution NMR spectroscopy. A variety of natural, derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular reaction progression, pathway kinetics, probe uptake and export, pH, redox state, reactive oxygen species, ion concentrations, drug efficacy or oncogenic signaling. These probes are readily used directly under natural conditions in biofluids and are often directly developed and optimized for cellular assays, thus leaving little doubt about their specificity and utility under biologically relevant conditions. Hyperpolarized molecular probes for biological NMR spectroscopy enable the unbiased detection of complex processes by virtue of the high spectral resolution, structural specificity and quantifiability of NMR signals. Here, we provide a survey of strategies used for the selection, design and use of hyperpolarized NMR probes in biological assays, and describe current limitations and developments. View Full-Text
Keywords: hyperpolarization; NMR; assays; bioprobes; designed probes; endogenous probes hyperpolarization; NMR; assays; bioprobes; designed probes; endogenous probes
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Meier, S.; Jensen, P.R.; Karlsson, M.; Lerche, M.H. Hyperpolarized NMR Probes for Biological Assays. Sensors 2014, 14, 1576-1597.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top