Ternary Nanostructure Coupling Flip-Flap Origami-Based Aptasensor for the Detection of Dengue Virus Antigens
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsThis work can be accepted in the present form.
Author Response
attached file
Author Response File: Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsThe paper “Ternary Nanostructure Coupling Flip-Flap Origami-Based Aptasensor for the Detection of Dengue Virus Antigen” by Mohd. Rahil Hasan and coauthors describes the development of the paper-based electrochemical aptasensor for the detection of the polyvalent antigen DENV.
A gold/Zn/graphene nanocomposite was used as the carrier of the analytical system; recognition and binding of the DENV antigen occurs due to a highly specific aptamer pre-sorbed here. The goal of the work is to create an effective test system suitable for use in POC conditions. It is important and relevant.
Some questions and comments:
1. Figure 5b: concentration of DENV varies in a range of 4 logarithmic units whereas the signal values – only in range of 2 times… Figure 5b: Ag-concentrations of 0.0001 mg/ml sharply change the signal magnitude, while a further sharp increase in antigen concentration does not cause such a drop. Is it normal? This needs to be discussed somehow.
- . Table 1, line 4 (DENV-Ag-Apt/Au/ZnO/GO-NC/oPAD): the target concentration (DENV-Ag) must be indicated.
- The term “Real sample” is not entirely correct - you did not use patient sera, but artificial samples prepared from sera and targets.
- References list should be compiled according to the Journal list.
Thus, the submitted manuscript needs some revision.
Author Response
attached file
Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for AuthorsPlease find attached comments and suggestions.
Comments for author File: Comments.pdf
Comments on the Quality of English LanguageMinor editing of English language required
Author Response
attached file
Author Response File: Author Response.pdf
Round 2
Reviewer 3 Report
Comments and Suggestions for AuthorsPlease find attached review report
Comments for author File: Comments.pdf
Comments on the Quality of English LanguageGrammatical errors should be improved.
Author Response
attached file
Author Response File: Author Response.docx