Next Article in Journal
A Chemoinformatics Approach to the Discovery of Lead-Like Molecules from Marine and Microbial Sources En Route to Antitumor and Antibiotic Drugs
Previous Article in Journal
Continuous Drug Release by Sea Anemone Nematostella vectensis Stinging Microcapsules
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Marine Drugs
Volume 12
Issue 2
10.3390/md12020746
marinedrugs-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Prenylated Indolediketopiperazine Peroxides and Related Homologues from the Marine Sediment-Derived Fungus Penicillium brefeldianum SD-273

by
Chun-Yan An
1,2,
Xiao-Ming Li
1,
Chun-Shun Li
1,
Gang-Ming Xu
1 and
Bin-Gui Wang
1,*
1
Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao 266071, China
2
University of Chinese Academy of Sciences, Yuquan Road 19A, Beijing 100049, China
*
Author to whom correspondence should be addressed.
Mar. Drugs 2014, 12(2), 746-756; https://doi.org/10.3390/md12020746
Submission received: 21 November 2013 / Revised: 10 January 2014 / Accepted: 15 January 2014 / Published: 27 January 2014
Browse Figures
Versions Notes

Abstract

:
Three new indolediketopiperazine peroxides, namely, 24-hydroxyverruculogen (1), 26-hydroxyverruculogen (2), and 13-O-prenyl-26-hydroxyverruculogen (3), along with four known homologues (4–7), were isolated and identified from the culture extract of the marine sediment-derived fungus Penicillium brefeldianum SD-273. Their structures were determined based on the extensive spectroscopic analysis and compound 1 was confirmed by X-ray crystallographic analysis. The absolute configuration of compounds 1–3 was determined using chiral HPLC analysis of their acidic hydrolysates. Each of the isolated compounds was evaluated for antibacterial and cytotoxic activity as well as brine shrimp (Artemia salina) lethality.

Graphical Abstract

1. Introduction

Indolediketopiperazine alkaloids are a series of natural occurring secondary metabolites generally formed from l-tryptophan and other common amino acids, e.g., l-proline, l-alanine, and d-valine [1,2,3,4,5,6,7,8]. Some of these compounds showed tremor-producing, cytotoxic, antibacterial, and brine shrimp lethal activity [3,4,5,6,7]. In our continuing investigation aimed to explore new and bioactive secondary metabolites from marine-derived fungi [9,10,11,12,13,14,15], we recently focused on a fungal strain Penicillium brefeldianum SD-273 that was isolated from the sediment samples collected from the estuary of the Pearl River in the South China Sea. The EtOAc extract of the fermentation broth showed moderate brine shrimp (Artemia salina) lethality in the preliminary assays. Further investigation of the culture extracts of this fungus resulted in the isolation of three new indolediketopiperazine peroxides (1–3) and four known homologues (4–7) (Figure 1). The structure of these compounds was determined by spectroscopic analysis and compound 1 was confirmed by X-ray crystallographic experiment. The absolute configuration of compounds 1–3 was determined by chiral HPLC analysis of their acidic hydrolysates. It should be noted that, based on our 1D and 2D NMR data, the original assignment of the carbon signals for the two methyls in the isoprenyl moiety of verruculogen (4) [4] should be interchanged. This paper describes the isolation, structure determination, and bioactivity evaluation of the isolated compounds.
Marinedrugs 12 00746 g001 1024
Figure 1. The structures of isolated compounds 1–7.
Figure 1. The structures of isolated compounds 1–7.
Marinedrugs 12 00746 g001

2. Results and Discussion

2.1. Structure Elucidation of the New Compounds 1–3

The cultured broth and mycelia of the fungus were exhaustively extracted with EtOAc and MeOH, respectively. Since the HPLC and TLC profiles of the two extracts were nearly identical, they were combined before further separation. The combined extract was fractionated by a combination of column chromatography (CC) on Si gel, reversed-phase Si gel C18, and Sephadex LH-20 as well as by semi-preparative HPLC and preparative thin layer chromatography (pTLC), to yield seven indolediketopiperazine derivatives (1–7) (Figure 1).
Compound 1 was obtained as colorless crystals. The HRESIMS experiment established its molecular formula C27H33N3O8. Inspection of the 1H-, 13C-, and DEPT NMR data of 1 revealed the presence of four methyls (with one O-methyl group), five sp3 methylenes (with one oxygenated), four sp3 and four sp2 methines, and ten quaternary carbons (with two oxygenated sp3 and six sp2 carbons and two ester/amide carbons), as well as three exchangeable protons (Table 1). The 1H- and 13C-NMR data assignments matched well with those of the corresponding signals for verruculogen (4), a tremorgenic mycotoxin peroxide isolated from peanuts-derived fungus Penicillium verruculosum [4,5], except for the presence of the C-24 hydroxy group, which was consistent with the difference in the molecular formula. This difference was supported by the fact that the NMR signals for one of the two methyls in the prenyl moiety of 4 [4,5] were replaced by the downfield oxygenated CH2 signals at δH 3.80/δC 65.2 (CH2-24) in the NMR spectra of 1 (Table 1). The HMBC correlations from H-22 to C-24 and C-25, from H2-24 to C-22 and C-23, and from H3-25 to C-22, C-23, and C-24 verified the above deduction (Figure 2). The observed NOE correlations from H-26α to H-3 and H-6 and from H-3 to H-22 indicated that these protons located on the same face of the molecule (Figure 3). In addition, NOE correlation from H-22 to the proton of 24-OH established the E-geometry for the double bond at C-22. However, the relative configuration for 12-OH and 13-OH was not assigned since no diagnostic NOE correlations could be detected.
Table
Table 1. 1H and 13C NMR data of compounds 1–3 a.
Table 1. 1H and 13C NMR data of compounds 1–3 a.
No.123
δH (J in Hz)δCδH (J in Hz)δCδH (J in Hz)δC
2 131.4, C 129.6, C 129.8, C
35.89, d (10.0)48.3, CH6.13, d (8.8)49.1, CH6.20, d (9.0)48.4, CH
5 171.1, C 170.7, C 171.4, C
64.45, t (8.0)58.8, CH4.35, t (7.9)58.7, CH4.45, t (8.2)58.9, CH
7α 2.30, m
β 1.88, m		29.3, CH2α 2.33, m
β 1.90, m		29.5, CH2α 2.30, m
β 1.87, m		29.2, CH2
81.94, m22.6, CH21.90, m22.1, CH21.87, m22.5, CH2
9α 3.44, m
β 3.50, t, 8.2		45.5, CH2α 3.44, m
β 3.52, m		44.8, CH2α 3.40, m
β 3.52, m		45.2, CH2
11166.5, C166.0, C165.5, C
1282.1, C82.6, C84.1, C
135.40, s68.4, CH5.43, s68.1, CH5.12, s73.5, CH
14 107.9, C 107.8, C 107.5, C
15 121.3, C 120.6, C 120.4, C
167.72, d (8.7)121.8, CH7.72, d (9.3)121.1, CH7.57, d (8.7)120.3, CH
176.69, dd (8.7, 1.3)109.3, CH6.69, dd (9.3, 2.1)108.7, CH6.74, dd (8.7, 2.2)108.8, CH
18 156.0, C 155.3, C 155.3, C
196.75, d (1.3)94.3, CH6.70, d (2.1)93.4, CH6.69, d (2.2)93.6, CH
20 136.3, C 135.3, C 135.3, C
216.88, d (8.4)85.5, CH6.78, d (8.2)85.0, CH6.77, d (8.2)85.1, CH
225.26, d (8.4)116.0, CH5.06, d (8.2)118.3, CH5.02, d (8.2)118.1, CH
23 146.9, C 142.8, C 143.0, C
243.80, s65.2 CH21.73, s25.2, CH31.74, s25.4, CH3
251.87, s14.5, CH32.00, s18.3, CH32.00, s18.4, CH3
26α 1.91, m
β 1.55, m		51.3, CH22.92, d (8.8)80.0, CH2.92, d (9.0)79.3, CH
2783.2, C 85.0, C 85.2, C
281.57, s24.6, CH31.44, s18.4, CH31.41, s18.3, CH3
290.95, s27.2, CH30.99, s25.3, CH30.98, s25.2, CH3
30 4.56, dd (7.0, 11.0)
4.88, dd (6.5, 11.0)		68.5, CH2
31 5.57, t (6.5)122.2, CH
32 134.5, C
33 1.75, s18.1, CH3
34 1.77, s25.2, CH3
18-OCH33.75, s55.8, CH33.76, s55.2, CH33.76, s55.2, CH3
12-OH5.10, br s b 5.26, br s b 6.16, br s
13-OH6.68, br s b 6.32, br s b
24-OH3.34, br s
26-OH 5.52, br s 5.37, br s
a Measured in DMSO-d6, at 500 MHz for 1H and 125 MHz for 13C with reference to the solvent signals, δ in ppm; b Exchangeable signals in the same column.
Marinedrugs 12 00746 g002 1024
Figure 2. Key HMBC (red arrows) and 1H–1H COSY (bold line) correlations of 1–3.
Figure 2. Key HMBC (red arrows) and 1H–1H COSY (bold line) correlations of 1–3.
Marinedrugs 12 00746 g002
Marinedrugs 12 00746 g003 1024
Figure 3. Key NOE correlations (double arrows) of 1–3.
Figure 3. Key NOE correlations (double arrows) of 1–3.
Marinedrugs 12 00746 g003
To unambiguously assign the structure, a single X-ray diffraction study was performed. However, compound 1 was difficult to get suitable crystal for X-ray analysis and after many attempts, X-ray quality crystals were obtained by slow evaporation of a solution of 1 in CHCl3-MeOH (1:1). The X-ray diffraction crystallographic analysis confirmed the structure and relative configuration for 1 (Figure 4).
The absolute configuration of 1 was deduced after chiral HPLC analysis of the degradation products of the acidic hydrolysate. The HPLC profile of the hydrolysate was compared with authentic standard to establish the configuration of the amino acid-derived unit as l-proline (Figure 5), corresponding to the S-configuration for C-6. The absolute configuration of compound 1 was therefore assigned to be 3S, 12R, 13S, and 21R. The structure for compound 1 was finally determined and it was named as 24-hydroxyverruculogen.
Marinedrugs 12 00746 g004 1024
Figure 4. X-ray structure of compound 1. (Note: A different numbering system is used for the structure in the text.)
Figure 4. X-ray structure of compound 1. (Note: A different numbering system is used for the structure in the text.)
Marinedrugs 12 00746 g004
Marinedrugs 12 00746 g005 1024
Figure 5. Chiral HPLC analysis of the amino acid derivatives of compounds 1–3 (Phenomenex-Chirex 3126 column, 250 × 4.60 mm, 5 μm; Gradient: 10% aqueous acetonitrile with 2 mM CuSO4·5H2O, detected at 225 nm).
Figure 5. Chiral HPLC analysis of the amino acid derivatives of compounds 1–3 (Phenomenex-Chirex 3126 column, 250 × 4.60 mm, 5 μm; Gradient: 10% aqueous acetonitrile with 2 mM CuSO4·5H2O, detected at 225 nm).
Marinedrugs 12 00746 g005
Compound 2 was isolated as a pale amorphous solid. Its molecular formula was demonstrated as C27H33N3O8 by HRESIMS data. Analysis of its 1H and 13C NMR spectral data (Table 1) with those of verruculogen (4) indicated that both compounds shared the same indolediketopiperazine skeleton [4,5]. The main difference between the two compounds was the methylene carbon resonating at δC 45.3 (C-26, this carbon was numbered as C-19 in the reference 4) in the 13C NMR spectrum of 4 [4] being replaced by an oxygenated methine (δC 80.0, C-26) in that of 2. This difference was verified by the 1H–1H COSY correlations from oxymethine proton H-26 to H-3 (δH 6.13, d, J = 8.8 Hz) and to the exchangeable proton signal at δH 5.52 (br s, 26-OH). The observed HMBC correlations from H-3, H3-28, and H3-29 to C-26 (Figure 2) supported this deduction. Other 1H–1H COSY and HMBC correlations confirmed the structure of 2 (Figure 2). However, the remaining two exchangeable protons resonating at δH 5.26 and 6.32, which belonging to 12-OH and 13-OH, could not be unambiguously ascribed since they didn’t show any correlations in the 2D NMR spectra.
The relative configuration of compound 2 was determined by NOESY experiment, proton coupling constant, and NMR data comparison. The trans-relationship between H-3 and H-26 was deduced on the basis of large coupling constant (J3,26 = 8.8 Hz) and by the fact that there is no NOE correlation could be detected among them in the NOESY spectrum. The NOE correlations from the proton of 26-OH to the protons of 12-OH and 13-OH implied the same direction of these OH groups, while the observed NOE correlation from H-3 to the olefinic H-22 indicated the same orientation of H-3 and CH-22 (Figure 2). Unfortunately, the NOESY data for compound 2 proved to be insufficient to solve the relative configuration at C-6. However, based on the fact of the very similar NMR data of H-6 to H-9 and of C-6 to C-9 (Table 1) in 1 and 2, as well as from the biogenetic consideration, the configuration of H-6 was assigned as α-orientation, the same as that of compound 1.
The absolute configuration of 2 was also determined by amino acid analysis of its acidic hydrolysate (Figure 5), which assigned S-configuration for C-6. The other chiral centers in 2 were therefore deduced as 3R, 12R, 13S, 21R, and 26S. The structure for compound 2 was thus determined and it was named as 26-hydroxyverruculogen.
Compound 3 was also isolated as a pale amorphous solid. Its molecular formula was determined to be C32H41N3O8 on the basis of HRESIMS data. Detailed comparison of the 1D and 2D NMR data of 3 with those of 2 revealed the same structural features, except for the presence of five additional carbon signals at δC 68.5 (CH2, C-30), 122.2 (CH, C-31), 134.5 (C, C-32), 18.1 (CH3, C-33), and 25.2 (CH3, C-34) in the 13C NMR spectrum of 3, which ascribable to the presence of a prenyl moiety in 3. The 1H NMR spectrum displayed the corresponding signals for the prenyl motif at δH 4.56/4.88 (H2-30), 5.57 (H-31), 1.75 (H3-33), and 1.77 (H3-34). The attachment of the prenyl unit at C-13 was evidenced by the observed HMBC correlation from H-13 to the oxygenated carbon C-30 (Figure 2).
The relative configuration of compound 3 was determined by proton coupling constant and NOESY spectrum. The large coupling constant (9.0 Hz) of H-3 and H-26 (Table 1) indicated that the two protons located on the different face of the molecule. The observed NOE correlations from 12-OH to 26-OH and H-9α and from H-9α to H-6 suggested the same orientation of these groups (Figure 3), while the correlations from H-3 to H-22 and from H-26 to H-13 indicated that H-3, H-22, and 13-O-isoprenyl units located on the same face with 26-OH. The absolute configuration of C-6 was also determined as S by chiral HPLC analysis of the hydralates and the absolute configuration of compound 3 was thus established as 3R, 6S, 12R, 13S, 21R, and 26S, same as that of 2. Compound 3 was named as 13-O-prenyl-26-hydroxyverruculogen.
In addition to the new compounds 1–3, four known homologues verruculogen (4) [4,5], fumitremorgin A (5) [2], cyclotryprostatin A (6) [3], and TR-2 (7) [4], were also isolated and identified from the culture extracts of P. brefeldianum SD-273.

2.2. Biological Activities of the Isolated Compounds

The isolated compounds 1–7 were examined for antibacterial, cytotoxicity, and brine shrimp lethality. None of them showed potent antibacterial activity against two bacteria (Escherichia coli and Staphyloccocus aureus), or cytotoxicity against eight tumor cell lines (B16, HuH-7, SW-1990, Hela, Du145, H460, MCF-7, and SGC-7901). However, compound 3 showed potent lethality against brine shrimp (Artemia salina), with LD50 value of 9.44 μΜ, while the positive control colchicine had the LD50 value of 99.0 μΜ, and the activity might be related to the prenyl substitution at 13-OH.

3. Experimental Section

3.1. General

The melting point was determined with an SGW X-4 micro-melting-point apparatus. The optical rotations were measured on an Optical Activity A-55 polarimeter. UV data were obtained on a Lengguang Gold S54 spectrophotometer. The 1H, 13C, and 2D NMR spectroscopic data were recorded on Bruker Advance 500 spectrometer. Mass spectra were measured on a VG Autospec 3000 mass spectrometer. HPLC analysis was performed using a Dionex HPLC System equipped with a P680 pump, an ASI-100 automated sample injector, a TCC-100 column oven, a UV-DAD 340U detector, and a Dionex Acclaim ODS column (4.6 × 250 mm, 5 μm). Semi-preparative HPLC was operated using a Dionex UltiMate U3000 system using an Agilent Prep RP-18 column (21.2 × 250 mm, 10 μm) with UV detection. A Phenomenex-Chirex 3126 N,S-dioctyl-(d)-penicillamine column (250 × 4.60 mm, 5 μm) was used for chiral HPLC analysis. Column chromatography (CC) was performed with silica gel (200–300 mesh, Qingdao Marine Chemical Factory, Qingdao, Shandong, China), Lobar LiChroprep RP-18 (40–63 μm; Merck, Darmstadt, Germany), and Sephadex LH-20 (18–110 μm, Merck, Darmstadt, Germany).

3.2. Fungal Material

The fungus Penicillium brefeldianum SD-273 was isolated from a sediment sample collected from the estuary of the Pearl River in South China Sea at a depth of 100 m, in October 2010. The fungal strain grew fast on potato dextrose agar plate and the pale yellow mycelia with few spores could be observed in about 3 days at 28 °C. Fungal identification was carried out using a molecular biological protocol by DNA amplification and sequencing of the ITS region as well as by calmodulin (cmd) sequencing as described previously [16]. The sequence data derived from the fungal strain have been submitted to and deposited at GenBank under accession no. JQ306332 (ITS) and KJ160447 (cmd). A BLAST search result showed that the ITS rDNA sequence was same (100%) to the sequence of Eupenicillium brefeldianum B37 (GenBank accession no. EF488446. It should be noted that the genus Eupenicilium is not used anymore and was re-defined to belong in Penicillium [17]), while the calmodulin sequence of the strain SD-273 was similar (99%) to that of Eupenicillium brefeldianum AS3.6689 (accession no. AY678593). The strain is preserved at China General Microbiological Culture Collection Center, CGMCC (Culture Collection Number CGMCC 7.160).

3.3. Fermentation

For chemical investigation, the fungal strain was statically cultivated in liquid potato-dextrose broth medium (1000 mL seawater, 20 g glucose, 5 g peptone, 3 g yeast extract, pH 6.5–7.0, liquid medium/flask = 300 mL) in 1 L Erlenmeyer flasks for 30 days at room temperature.

3.4. Extraction and Isolation

The fermented whole broth (300 mL × 100 flasks) was filtered through cheesecloth to separate the culture broth and mycelia, which were extracted with EtOAc and MeOH, respectively. The two extracts were combined for further separation since their HPLC and TLC profiles were almost identical. The combined extracts (45.1 g) were subjected to Si gel vacuum liquid chromatography (VLC) using CHCl3–MeOH gradient elution to get eight fractions (Frs.1–8). Fr.7 (5.0 g) was subjected to column chromatography (CC) on silica gel eluted with petroleum ether–EtOAc (from 20:1 to 1:1) to give eight subfractions (Frs.7.1–7.8). Fr.7.6 (67.0 mg) was further purified by CC on Sephadex LH-20 (MeOH) and preparative TLC to obtain compound 5 (17.9 mg). Fr.8 (6.3 g) was further separated by CC on reversed phase silica gel C18 with a MeOH–H2O gradient (from 20% to 100%) to get nine subfractions (Frs.8.1–8.9). Fr.8.5 (410.0 mg) was subjected to semi-preparative HPLC (Dionex UltiMate U3000, 21.2 × 250 mm, 10 μm, 85% aqueous MeOH, 16 mL/min) to afford compounds 6 (99.0 mg, tR 10.2 min) and 4 (44.0 mg, tR 12.1 min). Fr.8.7 (230.0 mg) was purified by preparative TLC (CHCl3–MeOH, 15:1) to yield compounds 1 (16.0 mg) and 2 (14.0 mg). Fr.8.9 (224.0 mg) was further subjected to CC on Sephadex LH-20 to obtain compounds 3 (8.6 mg) and 7 (5.4 mg).
24-Hydroxyverruculogen (1): colorless crystals (CH3Cl–MeOH, 1:1); mp 246–248 °C; Marinedrugs 12 00746 i001 −12.5 (c 0.24, MeOH); UV (MeOH) λmax (log ε) 223 (4.51), 268 (3.70), 288 (3.64) nm; 1H and 13C NMR data, see Table 1; ESIMS m/z 550 [M + Na]+; HRESIMS m/z 550.2153 [M + Na]+ (calcd for C27H33N3O8Na+, 550.2165, Δ −1.2 mmu).
26-Hydroxyverruculogen (2): pale amorphous solid (MeOH); Marinedrugs 12 00746 i001 −75.5 (c 0.23, MeOH); UV (MeOH) λmax (log ε) 223 (4.45), 271 (3.59), 287 (3.54) nm; 1H and 13C NMR data, see Table 1; ESIMS m/z 550 [M + Na]+; HRESIMS m/z 550.2156 [M + Na]+ (calcd for C27H33N3O8Na+, 550.2165, Δ −0.9 mmu).
13-O-Prenyl-26-hydroxyverruculogen (3): pale amorphous solid (MeOH); Marinedrugs 12 00746 i001 −10.1 (c 0.35, MeOH); UV (MeOH) λmax (log ε) 227 (4.60), 275 (3.88), 289 (3.80) nm; 1H and 13C NMR data, see Table 1; ESIMS m/z 618 [M + Na]+; HRESIMS m/z 618.2804 [M + Na]+ (calcd for C32H41N3O8Na+, 618.2791, Δ +1.3 mmu).

3.5. X-ray Crystallographic Analysis of Compound 1

All crystallographic data [18] were collected on a Bruker Smart-1000 CCD diffractometer equipped with graphite-monochromatic Mo Kα radiation (λ = 0.71073 Å) at 293(2) K. The data were corrected for absorption by using the program SADABS [19]. The structure was solved by direct methods with the SHELXTL software package [20]. All non-hydrogen atoms were refined anisotropically. The H atoms were located by geometrical calculations, and their positions and thermal parameters were fixed during the structure refinement. The structure was refined by full-matrix least-squares techniques [21].
Crystal data for 1: C27H33N3O8; F.W. = 527.56; orthorhombic space group P2(1)2(1)2(1); unit cell dimensions a = 11.0113(9) Å, b = 13.2224(8) Å, c = 17.6195(7) Å, V = 2565.3(3) Å3, α = β = γ = 90°, Z = 4, dcalcd = 1.366 mg/m3, crystal dimensions 0.35 × 0.18 × 0.12 mm, μ = 0.101 mm−1, F(000) = 1120. The 6266 measurements yielded 4426 independent reflections after equivalent data were averaged, and Lorentz and polarization corrections were applied. The final refinement gave R1 = 0.0604 and wR2 = 0.0972 [I > 2σ(I)].

3.6. Amino Acid Analysis

Compounds 1 (1.0 mg), 2 (0.9 mg), and 3 (0.8 mg) were each dissolved in 10 mL 6 N HCl and heated at 110 °C for 24 h in sealed tubes. The solutions were then evaporated to dryness under reduced pressure. Each sample, including the standard amino acids l-pro and d-pro, was dissolved in 1 mL of eluting solvent (2 mM CuSO4·5H2O, with 5 mL CH3CN in every 100 mL solvent) and was centrifuged at a speed of 12,000 rpm to get corresponding supernate for chiral HPLC analysis, which was carried out using a Phenomenex-Chirex-3126 column at 254 nm with flow rate 1.0 mL/min at 40 °C. The HPLC analysis showed that the products of acidic hydrolysis of 1–3 contained the identical amino acids, which have the same retention time as that of the standard l-pro. The results established the chiral center of the proline moiety in the structure of the three compounds to be S-configuration (Figure 5).

3.7. Brine Shrimp Toxicity

Brine shrimp (A rtemia salina) toxicity of the isolated compounds was determined as described previously [22]. Colchicine was used as a positive control.

3.8. Cytotoxicity Assay

The cytotoxic activity against B16 (murine melanoma), HuH-7 (hepatocarcinoma), SW-1990 (human pancreatic adenocarcinoma), Hela (human epithelial carcinoma), Du145 (human prostate carcinoma), H460 (non-small cell lung cancer), MCF-7 (human breast adenocarcinoma), and SGC-7901 (human gastric cancer) cell lines were determined according to previously reported methods [23].

3.9. Antibacterial Assay

Antibacterial assay against E. coli and S. aureus was carried out using the well diffusion method [24]. Chloromycetin was used as a positive control.

4. Conclusions

In summary, three new indolediketopiperazine derivatives (1–3) as well as four known related homologues (4–7) were isolated and identified from the culture extract of the marine sediment-derived fungus P. brefeldianum SD-273 and the structures and absolute configuration were determined based on the extensive interpretation of spectroscopic data, X-ray crystallographic diffraction, and chiral HPLC analysis. Although none of these compounds showed potent antibacterial activity or cytotoxicity, compound 3 showed potent lethal activity against brine shrimp.

Acknowledgments

Financial support from the Ministry of Science and Technology of China (2013AA092901 and 2012AA092104) and from the Natural Science Foundation of China (31270403) is gratefully acknowledged. The authors appreciate Y.-S. Wang at the South China Sea Institute of Oceanology of the Chinese Academy of Sciences for his help in sediment sampling.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Wang, W.L.; Lu, Z.Y.; Tao, H.W.; Zhu, T.J.; Fang, Y.C.; Gu, Q.Q.; Zhu, W.M. Isoechinulin-type alkaloids, variecolorins A–L, from halotolerant Aspergillus variecolor. J. Nat. Prod. 2007, 70, 1558–1564. [Google Scholar] [CrossRef]
  2. Yamazaki, M.; Fujimoto, H.; Kawasaki, T. Chemistry of tremorogenic metabolites. I. Fumitremorgin A from Aspergillus fumigatus. Chem. Pharm. Bull. 1980, 28, 245–254. [Google Scholar] [CrossRef]
  3. Cui, C.B.; Kakeya, H.; Osada, H. Novel mammalian cell cycle inhibitors, tryprostatins A, B and other diketopiperazines produced by Aspergillus fumigatus II. Physico-chemical properties and structures. J. Antibiot. 1996, 49, 534–540. [Google Scholar] [CrossRef]
  4. Cole, R.J.; Kirksey, J.W.; Cox, R.H.; Clardy, J. Structure of the tremor-producing indole, TR-2. J. Agric. Food Chem. 1975, 23, 1015–1018. [Google Scholar] [CrossRef]
  5. Fayos, J.; Lokensgard, D.; Clardy, J.; Cole, R.J.; Kirksey, J.W. Structure of verruculogen, a tremor producing peroxide from Penicillium verruculosum. J. Am. Chem. Soc. 1974, 21, 6785–6787. [Google Scholar]
  6. Du, F.Y.; Li, X.M.; Li, C.S.; Shang, Z.; Wang, B.G. Cristatumins A–D, new indole alkaloids from the marine-derived endophytic fungus Eurotium cristatum EN-220. Bioorg. Med. Chem. Lett. 2012, 22, 4650–4653. [Google Scholar] [CrossRef]
  7. Wang, F.; Fang, Y.; Zhu, T.; Zhang, M.; Lin, A.; Gu, Q.; Zhu, W. Seven new prenylated indole diketopiperazine alkaloids from holothurian-derived fungus Aspergillus fumigatus. Tetrahedron 2008, 64, 7986–7991. [Google Scholar] [CrossRef]
  8. Willingale, J.; Perera, K.P.W.C.; Mantle, P.G. An intermediary role for the tremorgenic mycotoxin TR-2 in the biosynthesis of verruculogen. Biochem. J. 1983, 214, 991–993. [Google Scholar]
  9. An, C.Y.; Li, X.M.; Li, C.S.; Wang, M.H.; Xu, G.M.; Wang, B.G. Aniquinazolines A–D, four new quinazolinone alkaloids from marine-derived endophytic fungus Aspergillus nidulans. Mar. Drugs 2013, 11, 2682–2694. [Google Scholar] [CrossRef]
  10. Wang, M.H.; Li, X.M.; Li, C.S.; Ji, N.Y.; Wang, B.G. Secondary metabolites from Penicillium pinophilum SD-272, a marine sediment-derived fungus. Mar. Drugs 2013, 11, 2230–2238. [Google Scholar] [CrossRef]
  11. Li, C.S.; Li, X.M.; Gao, S.S.; Lu, Y.H.; Wang, B.G. Cytotoxic anthranilic acid derivatives from deep sea sediment-derived fungus Penicillium paneum SD-44. Mar. Drugs 2013, 11, 3068–3076. [Google Scholar] [CrossRef]
  12. Zhao, Y.; Chen, H.; Shang, Z.; Jiao, B.; Yuan, B.; Sun, W.; Wang, B.; Miao, M.; Huang, C. SD118-xanthocillin X (1), a novel marine agent extracted from Penicillium commune, induces autophagy through the inhibition of the MEK/ERK pathway. Mar. Drugs 2012, 10, 1345–1359. [Google Scholar] [CrossRef]
  13. An, C.Y.; Li, X.M.; Li, C.S.; Gao, S.S.; Shang, Z.; Wang, B.G. Triazoles and other N-containing metabolites from the marine-derived endophytic fungus Penicillium chrysogenum EN-118. Helv. Chim. Acta 2013, 96, 682–687. [Google Scholar] [CrossRef]
  14. Zhang, Y.; Li, X.M.; Shang, Z.; Li, C.S.; Ji, N.Y.; Wang, B.G. Cytoglobosins A–G, cytochalasans from a marine-derived endophytic fungus, Chaetomium globosum QEN-14. J. Nat. Prod. 2010, 73, 729–733. [Google Scholar]
  15. Sun, H.F.; Li, X.M.; Meng, L.; Cui, C.M.; Gao, S.S.; Li, C.S.; Huang, C.G.; Wang, B.G. Asperolides A–C, tetranorlabdane diterpenoids from the marine alga-derived endophytic fungus Aspergillus wentii EN-48. J. Nat. Prod. 2012, 75, 148–152. [Google Scholar]
  16. Wang, S.; Li, X.M.; Teuscher, F.; Li, D.L.; Diesel, A.; Ebel, R.; Proksch, P.; Wang, B.G. Chaetopyranin, a benzaldehyde derivative, and other related metabolites from Chaetomium globosum, an endophytic fungus derived from the marine red alga Polysiphonia urceola. J. Nat. Prod. 2006, 69, 1622–1625. [Google Scholar]
  17. Houbraken, J.; Samson, R.A. Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Stud. Mycol. 2011, 70, 1–51. [Google Scholar] [CrossRef]
  18. Cambridge Crystallographic Data Centre (CCDC). Deposition No. CCDC923360. Available online: http://www.ccdc.cam.ac.uk/data_request/cif (accessed on 6 February 2013).
  19. Sheldrick, G.M. SADABS, Software for Empirical Absorption Correction; University of Göttingen: Göttingen, Germany, 1996. [Google Scholar]
  20. Sheldrick, G.M. SHELXTL, Structure Determination Software Programs; Bruker Analytical X-ray System Inc.: Madison, WI, USA, 1997. [Google Scholar]
  21. Sheldrick, G.M. SHELXL-97 and SHELXS-97,Program for X-ray Crystal Structure Solution and Refinement; University of Göttingen: Göttingen, Germany, 1997. [Google Scholar]
  22. Meyer, B.N.; Ferrigni, N.R.; Putnam, J.E.; Jacobsen, L.B.; Nichols, D.E.; McLaughlin, J.L. Brine shrimp: A convenient general bioassay for active plant constituents. Planta Med. 1982, 45, 31–34. [Google Scholar] [CrossRef]
  23. Bergeron, R.J.; Cavanaugh, P.F., Jr.; Kline, S.J.; Hughes, R.G., Jr.; Elliott, G.T.; Porter, C.W. Antineoplastic and antiherpetic activity of spermidine catecholamide iron chelators. Biochem. Biophys. Res. Commun. 1984, 121, 848–854. [Google Scholar] [CrossRef]
  24. Al-Burtamani, S.K.S.; Fatope, M.O.; Marwah, R.G.; Onifade, A.K.; Al-Saidi, S.H. Chemical composition, antibacterial and antifungal activities of the essential oil of Haplophyllum tuberculatum from Oman. J. Ethnopharmacol. 2005, 96, 107–112. [Google Scholar] [CrossRef]

Share and Cite

MDPI and ACS Style

An, C.-Y.; Li, X.-M.; Li, C.-S.; Xu, G.-M.; Wang, B.-G. Prenylated Indolediketopiperazine Peroxides and Related Homologues from the Marine Sediment-Derived Fungus Penicillium brefeldianum SD-273. Mar. Drugs 2014, 12, 746-756. https://doi.org/10.3390/md12020746

AMA Style

An C-Y, Li X-M, Li C-S, Xu G-M, Wang B-G. Prenylated Indolediketopiperazine Peroxides and Related Homologues from the Marine Sediment-Derived Fungus Penicillium brefeldianum SD-273. Marine Drugs. 2014; 12(2):746-756. https://doi.org/10.3390/md12020746

Chicago/Turabian Style

An, Chun-Yan, Xiao-Ming Li, Chun-Shun Li, Gang-Ming Xu, and Bin-Gui Wang. 2014. "Prenylated Indolediketopiperazine Peroxides and Related Homologues from the Marine Sediment-Derived Fungus Penicillium brefeldianum SD-273" Marine Drugs 12, no. 2: 746-756. https://doi.org/10.3390/md12020746

Article Metrics

Back to TopTop