Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163
Abstract
:1. Introduction
2. Results and Discussion
2.1. Monoculture Chemical Profiles
2.2. Co-Culture Chemical Profile
2.3. Anti-Infective Activity of Induced Metabolites
3. Experimental Section
3.1. General Experimental Procedures
3.2. Microbial Fermentation and Extracts Preparation
3.3. Extraction and Isolation
3.3.1. General Chromatographic Procedures for Large-Scale Isolation and Fingerprinting Work
3.3.2. Actinokineospora sp. EG49
3.3.3. Nocardiopsis sp. RV163
3.3.4. Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 Co-Culture
3.4. Bioactivity Testing
3.4.1. Antibacterial Activity
3.4.2. Anti-Trypanosomal Activity
- Y1
- mean of the duplicate determination of the first measured cell density that is less than half the average of the growth control divided by the average of control growth.
- X1
- concentration of the substance that belongs to the cell density of Y1.
- Y2
- mean of the duplicate determination of the first measured cell density that is greater than half the average of the growth control divided by the average of control growth.
- X2
- concentration of the substance that belongs to the cell density of Y2.
3.4.3. Anti-Leishmanial Activity
3.5. Structure Elucidation of Compounds 1–13
4. Conclusions
Supplementary Files
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Dashti, Y.; Grkovic, T.; Abdelmohsen, U.R.; Hentschel, U.; Quinn, R.J. Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Mar. Drugs 2014, 12, 3046-3059. https://doi.org/10.3390/md12053046
Dashti Y, Grkovic T, Abdelmohsen UR, Hentschel U, Quinn RJ. Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Marine Drugs. 2014; 12(5):3046-3059. https://doi.org/10.3390/md12053046
Chicago/Turabian StyleDashti, Yousef, Tanja Grkovic, Usama Ramadan Abdelmohsen, Ute Hentschel, and Ronald J. Quinn. 2014. "Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163" Marine Drugs 12, no. 5: 3046-3059. https://doi.org/10.3390/md12053046