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Review

Selective Blocking Effects of 4,9-Anhydrotetrodotoxin, Purified from a Crude Mixture of Tetrodotoxin Analogues, on NaV1.6 Channels and Its Chemical Aspects

by
Noriyoshi Teramoto
1,2,* and
Mari Yotsu-Yamashita
3
1
Department of Pharmacology, Faculty of Medicine, Saga University, Saga 849-8501, Japan
2
Laboratory of Biomedical Engineering, Graduate School of Biomedical Engineering, Tohoku University, Sendai 980-8575, Japan
3
Laboratory of Bioorganic Chemistry of Natural Products, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan
*
Author to whom correspondence should be addressed.
Mar. Drugs 2015, 13(2), 984-995; https://doi.org/10.3390/md13020984
Submission received: 24 December 2014 / Revised: 30 January 2015 / Accepted: 3 February 2015 / Published: 12 February 2015
(This article belongs to the Special Issue Emerging Marine Toxins)
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Abstract

:
Tetrodotoxin (TTX) is a potent neurotoxin found in a number of marine creatures including the pufferfish, where it is synthesized by bacteria and accumulated through the food chain. It is a potent and selective blocker of some types of voltage-gated Na+ channel (NaV channel). 4,9-Anhydrotetrodotoxin (4,9-anhydroTTX) was purified from a crude mixture of TTX analogues (such as TTX, 4-epiTTX, 6-epiTTX, 11-oxoTTX and 11-deoxyTTX) by the use of liquid chromatography-fluorescence detection (LC-FLD) techniques. Recently, it has been reported that 4,9-anhydroTTX selectively blocks the activity of NaV1.6 channels with a blocking efficacy 40–160 times higher than that for other TTX-sensitive NaV1.x channel isoforms. However, little attention has been paid to the molecular properties of the α-subunit in NaV1.6 channels and the characteristics of binding of 4,9-anhydroTTX. From a functional point of view, it is important to determine the relative expression of NaV1.6 channels in a wide variety of tissues. The aim of this review is to discuss briefly current knowledge about the pharmacology of 4,9-anhydroTTX, and provide an analysis of the molecular structure of native NaV1.6 channels. In addition, chemical aspects of 4,9-anhydroTTX are briefly covered.

1. Introduction

Tetrodotoxin (TTX), one of several marine neurotoxins, was first isolated from pufferfish [1] and subsequently discovered in other marine organisms (such as octopus, flatworms, snails and crabs) as well as small terrestrial animals (newts and frogs). This neurotoxin is a potent and highly selective inhibitor of voltage-gated Na+ channels (NaV channels). Since its discovery, TTX has been utilized widely as a pharmacological tool for investigating the biophysical properties of NaV channels [2]. TTX selectively blocks the pore of the NaV channel from the extracellular side of the plasma membrane, thereby preventing the influx of Na+, without affecting other ion channels and receptors [3].
4,9-AnhydroTTX, which possesses a weaker toxic action than other TTX analogues, was first isolated from pufferfish liver using high performance liquid chromatography (HPLC; [4]). In comparison with the potency of TTX, 4,9-anhydroTTX was found to exhibit weaker blocking effects on NaV channels in squid axon [5] and lower affinity binding to rat synaptic membrane preparations containing NaV channels [6]. Subsequently, 4,9-anhydroTTX has been quantified using liquid chromatography fluorescence detection (LC-FLD; [7]) and liquid chromatography mass spectrometry (LC/MS) methods [8,9,10,11,12]. However, details of the inhibitory effects of 4,9-anhydroTTX on the activity of NaV channels have remained elusive. Using electrophysiological techniques, heterologous expression studies of seven different types of NaV1.x channels (NaV1.2–NaV1.8 channels) in Xenopus oocytes revealed that 4,9-anhydroTTX was a highly selective blocker of the NaV1.6 channel isoform, with a blocking efficacy 40–160 times higher than for other TTX-sensitive NaV1.x channel isoforms, suggesting that 4,9-anhydroTTX could be an invaluable tool for identifying NaV1.6 channel-mediated functions [13]. It has been reported recently that NaV1.6 channel-mediated resurgent-like NaV currents recorded in freshly isolated smooth muscle cells of the mouse vas deferens, were reversibly abolished by 4,9-anhydroTTX [14]. This observation strongly suggests that 4,9-anhydroTTX inhibits the channel activity of native NaV1.6 channels with a similar potency to that seen in NaV1.6 channel expression studies [13]. Classically, NaV channels have been classified into TTX-sensitive and TTX-resistant types, according to the potency and selectivity of TTX [2]. We believe that the development of selective TTX analogues that act on specific NaV channels, and hence show minimal toxicity, will provide highly useful pharmacological tool and be of great therapeutic interest.
In this review, we will focus on the target NaV channels for 4,9-anhydroTTX (i.e., NaV1.6 channels), and discuss the selective blocking effects of 4,9-anhydroTTX on NaV1.6 channel-mediated signals. In addition, certain chemical aspects of 4,9-anhydroTTX will be briefly covered.

2. NaV Channels

NaV channels are members of an ion channel protein superfamily that are widely expressed in both neuronal and non-neuronal cells. The essential role of NaV channels is in the generation of the action potential upstroke (i.e., the initiation and propagation of action potentials) in excitable cells (such as nerve fibers, skeletal muscle fibers, cardiac myocytes and some smooth muscle cell types). NaV channels also influence subthreshold electrical activity through persistent and resurgent NaV currents [15]. Additionally, recent studies have revealed that certain NaV channels may be involved in the processes underlying normal and pathological pain [16], and that they may be up-regulated in cancer, in general favoring invasive/metastatic phenotypes [17]. Thus, it is generally believed that NaV channels play important roles in various physiological and pathophysiological conditions [18].
In the 1980s, two proteins with substantially different biochemical properties (i.e., 260 kDa and 30–40 kDa) were detected in NaV channels isolated from mammalian skeletal muscle and brain [19,20]. Later cloning and heterologous expression studies revealed that the α subunit (260 kDa) forms the core of the channel (i.e., the channel pore) and is responsible for voltage-dependent gating, ionic selectivity and TTX-binding, and that the β subunits (30–40 kDa) act as auxiliary subunits to modify channel function [21]. As shown in Figure 1, the molecular structure of NaV channels comprises one α subunit and one or more β subunits.
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Figure 1. Molecular structure of NaV channels. (A) Schematic illustration of the predicted topology of NaV channels (α subunit and β subunits). The transmembrane domain model proposed by Catterall (2000) is shown [18]; (B) The α subunit is formed by four homologous domains (DI–DIV). Each domain consists of six α-helical transmembrane segments (S1–S6). P: channel pore.
Figure 1. Molecular structure of NaV channels. (A) Schematic illustration of the predicted topology of NaV channels (α subunit and β subunits). The transmembrane domain model proposed by Catterall (2000) is shown [18]; (B) The α subunit is formed by four homologous domains (DI–DIV). Each domain consists of six α-helical transmembrane segments (S1–S6). P: channel pore.
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To date, nine genes (Scn1a–5a and Scn8a–11a, comprising highly homologous clones) encoding the α subunits of TTX-sensitive (NaV1.1–NaV1.4, NaV1.6 and NaV1.7 channels, respectively) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9 channels, respectively) NaV channels have been identified within a single family of NaV channels, NaV1.x channels (Table 1; [22]). Two other genes (Scn6a and Scn7a) encoding NaX (also known as NaG or NaV2.1 channel) have also been identified; these have functional motifs that are related to NaV channels (such as the voltage-sensing and interdomain regions), but not the NaV channel pore itself (these channels have not been functionally expressed as yet) [22]. The latter isoforms are thought to be involved in the transport or absorption of Na+, by sensing the Na+ concentration gradient across the plasma membrane; therefore, they have been suggested to act as a “concentration-sensitive” Na+ influx protein [23,24].
Table
Table 1. Location and distribution of NaV channel genes. S, sensitive; R, resistant; CNS, central nervous system; PNS, peripheral nervous system; SMCs, smooth muscle cells.
Table 1. Location and distribution of NaV channel genes. S, sensitive; R, resistant; CNS, central nervous system; PNS, peripheral nervous system; SMCs, smooth muscle cells.
α Subunit (Channel pore)Coding geneTTX-SensitivityPredominant location
NaV1.1Scn1ASCNS, PNS
NaV1.2Scn2ASCNS
NaV1.3Scn3ASCNS (embryonic)
NaV1.4Scn4ASSkeletal muscle
NaV1.5Scn5ARHeart muscle
NaV1.6Scn8ASCNS, PNS, Glia, Nodes of Ranvier, SMCs
NaV1.7Scn9ASPNS, Schwann cells
NaV1.8Scn10ARPNS (sensory neurons)
NaV1.9Scn11ARPNS
Functional expression of the α subunit is required for the formation of the channel pore and ion selectivity filter, and this subunit determines several biophysical properties of the NaV channel [18]. As shown in Figure 2, the α subunit has four homologous domains (DI–DIV), each containing six transmembrane α-helical segments (S1–S6), which are connected by extracellular and intracellular loop regions. Specific amino acid sequences of the α subunit form the channel pore, voltage sensor, inactivation gates and various phosphorylation sites [25]. It is generally believed that TTX binds within the outer vestibule of the NaV channel to multiple residues in the α subunit that controls Na+ permeation (the P loop; between S5 and S6 in each domain is a short segment that appears to fold half-way into the membrane, like a hairpin structure); furthermore, TTX is thought to block the influx of Na+ by occluding the outer pore [26].
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Figure 2. Schematic representations of the α and β subunit s of NaV channels. The α subunit is formed by four homologous domains (DI–IV). Each domain consists of six α-helical transmembrane segments (S1–S6). P loops are located between segment 5 and 6, which are the pore-lining regions (yellow and green). TTX binding sites are shown in red. The β subunits consist of an N-terminal containing an immunoglobulin-like loop and the splice site, a C-terminal, and a membrane-spanning segment.
Figure 2. Schematic representations of the α and β subunit s of NaV channels. The α subunit is formed by four homologous domains (DI–IV). Each domain consists of six α-helical transmembrane segments (S1–S6). P loops are located between segment 5 and 6, which are the pore-lining regions (yellow and green). TTX binding sites are shown in red. The β subunits consist of an N-terminal containing an immunoglobulin-like loop and the splice site, a C-terminal, and a membrane-spanning segment.
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The β1–β4 isoforms of the β subunits are encoded by four genes (Scn1b–4b), with two splice variants of Scn1b (Scn1b-a and Scn1b-b) encoding β1-A and β1-B, respectively. The β subunits share a common structure [27] consisting of a single membrane-spanning domain, a small intracellular C-terminal domain, and a large extracellular N-terminal domain incorporating an immunoglobulin-like fold that is similar to that found in cell adhesion molecules (Figure 1; [21]). Recent studies have revealed that the β subunits play significant roles in the regulation of channel localization and in interactions with cell adhesion molecules, the extracellular matrix, intracellular cytoskeletal proteins and other molecules that modulate cell migration and aggregation [28].

3. Localization of NaV1.6 Channels

Approximately two decades ago, analysis of a transgene insertion site led to the isolation of a novel NaV channel gene designated Scn8a [29]. Scn8a encodes a 1732-amino acid member of the α subunit gene family of NaV channels (i.e., the NaV1.6 channel). Expression of Scn8a has been shown in the brain and spinal cord [29], with a widespread distribution in many regions of the central nervous system (CNS), including cerebellar granule cells, and pyramidal and granule cells of the hippocampus [30]. The human Scn8a gene has been mapped to a conserved linkage group on chromosome 12q13, and is a candidate gene for inherited neuromuscular disease [29].
The NaV1.6 isoform was initially detected in the nodes of Ranvier in the peripheral nervous system (PNS) and CNS [31,32], and along non-myelinated axons [33], suggesting that this type of NaV channel plays an important role in the function and regulation of both the PNS and CNS. NaV1.6 channels have been proposed to mediate resurgent and persistent Na+ currents with a resulting effect on repetitive firing behavior [34]. The NaV1.6 channel is also widely distributed throughout the spinal cord [35]. Its expression in the dorsal root ganglion (DRG) is predominantly in large myelinated A-fiber neurons [36]. It has been reported that NaV1.6 channels are also expressed in the axons that comprise small nerve bundles underlying the epidermis, and in epidermal free nerve terminals, including nociceptors [37]. Furthermore, NaV1.6 channels are expressed in keratinocytes, which may contribute to the sensation of pain. It is noteworthy that a significantly increased expression of NaV1.6 channels has been found in human skin biopsies taken from patients with complex regional pain syndrome and post-herpetic neuralgia [38]. These findings suggest a major role for NaV1.6 channels in the function of small-diameter sensory nerve endings and the related pathophysiology. In addition, several studies have provided strong evidence that the NaV1.6 channel is the predominant NaV channel isoform expressed in microglia, which are known to play an important role in pathological pain, and that NaV1.6 channels contribute to the response of microglia by multiplying various activating signals [39].

4. NaV1.6 Channel-Null Mice (med Mice) Lacking Expression of Scn8a

The loss of Scn8a expression in the mouse represents a unique and useful animal model for studying NaV1.6 channel-mediated phenomena (including ethology, functional analysis and morphological studies). It has been reported that mutations at the “motor endplate disease” (med) locus on distal chromosome 15 of the mouse results in a recessive neuromuscular disorder [40]. Analysis of a transgene-induced mutation at the mouse med locus was performed to identify the NaV1.6 channel gene, Scn8a [29]. The original med mutation (C3HeB/FeJ-Scn8amed) arose in Edinburgh, Scotland in 1958, and the medJ allele (C3HeB/FeJ-Scn8amed/J) was identified at The Jackson Laboratory in the USA [41]. The med mutation is caused by the insertion of a truncated long interspersed element (LINE) into exon 2 of Scn8a. The med transcript is spliced from exon 1 to a cryptic acceptor site in intron 2, whilst a 4 base-pair deletion within the 5′ donor site of exon 3 in the medJ allele results in splicing from exon 1 to exon 4. Both mutant transcripts possess altered reading frames with premature stop codons, which are close to the protein N terminus. The loss of Scn8a expression leads to progressive paralysis and early death.
Homozygous mice with a NaV1.6 null allele (med mice, NaV1.6−/−) have been widely utilized to investigate the molecular and functional contributions of NaV1.6 channel-mediated mechanisms. The med and medJ mutations both result in severe, progressive skeletal muscle atrophy, secondary to a loss of the functional innervation [42,43], that is lethal within three to four weeks of birth [41]. Although the number of motor neurons in the spinal cord of mutant mice is not reduced, transmission of excitatory activity across the neuromuscular junction is impaired. The med allele also produces a cerebellar ataxia, which is associated with a loss of spontaneous electrical activity in cerebellar Purkinje cells [44]. In smooth muscle, the electrophysiological properties of NaV currents through NaV channels have been compared between NaV1.6-null mice (NaV1.6−/−) lacking the expression of Scn8a and their wild-type littermates (NaV1.6+/+), leading to the conclusion that the smooth muscle-type NaV channel is likely to be NaV1.6 [14,45,46].
Pharmacological inhibition of NaV1.6 channel activity represents an alternative to the NaV1.6 channel-null mouse model. 4,9-AnhydroTTX is a highly selective toxin for the NaV1.6 channel, with an IC50 for this isoform that is 40–160 times lower than that for other TTX-sensitive NaV1.x isoforms (IC50 = 7.8 nM for the NaV1.6 channel, compared with 1260 nM for the NaV1.2 channel, 341 nM for the NaV1.3 channel, 988 nM for the NaV1.4 channel and 1270 nM for the NaV1.7 channel) [13]. Although much higher concentrations of 4,9-anhydroTTX (>300 nM) will inhibit the activity of other NaV1.x channels, appropriate concentrations of 4,9-anhydroTTX are considered to represent a convenient and selective pharmacological tool for assessing the function of the NaV1.6 channel.
Much higher concentrations of 4,9-anhydroTTX are required to inhibit the activities of TTX-insensitive NaV channels (i.e., NaV1.5 and NaV1.8 channels) compared with those needed to inhibit TTX-sensitive NaV channels [13]. Based on the available observations, it is reasonable to propose that 4,9-anhydroTTX likely blocks NaV channels through the same site(s) and inhibitory mechanism(s) as TTX. However, the exact binding site(s) for 4,9-anhydroTTX in NaV1.6 channels remain(s) elusive [13]. Further studies, examining the amino sequence alignments of the binding sites, are needed to characterize the mechanisms underlying the sensitivity to 4,9-anhydroTTX.

5. Chemical and Toxicological Aspects of 4,9-AnhydroTTX

TTX [47,48,49] is usually present as a mixture of TTX, 4-epiTTX and 4,9-anhydroTTX in an acidic aqueous solution, due to the existence of a chemical equilibrium between these compounds (Figure 3). It has been reported that both TTX and 4,9-anhydroTTX epimerize slowly to give an equilibrium mixture of TTX and 4,9-anhydroTTX in a ~4:1 ratio (NMR) [49]. As a result, the maximal purity of 4,9-anhydroTTX assessed by LC-FLD is ~99% [14], since small amounts of both TTX (0.5%) and 4-epiTTX (0.5%) are present due to their conversion from 4,9-anhydroTTX under acidic conditions. NMR data for TTX [50,51] and 4,9-anhydroTTX [4,52] have been reported. The major difference between TTX and 4,9-anhydroTTX is in the 3JH4/H4a value, which is 10 Hz for TTX and 0 Hz for 4,9-anhydroTTX. An LC-FLD approach for the quantitation of TTX and 4,9-anhydroTTX has been described [7], whilst we have developed an alternative LC/MS method [8,9,10,11,12].
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Figure 3. The interconversion of TTX, 4-epiTTX and 4,9-anhydroTTX resulting from the chemical equilibrium.
Figure 3. The interconversion of TTX, 4-epiTTX and 4,9-anhydroTTX resulting from the chemical equilibrium.
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Both 4-epiTTX and 4,9-anhydroTTX were successfully isolated from the pufferfish [4], and their toxicities determined in mice (ddY strain, male, 19–20 g, intraperitoneal administration; TTX: 4500 mouse units (MU)/mg; 4-epiTTX: 710 MU/mg; 4,9-anhydroTTX: 92 MU/mg). We have also determined the affinities of these compounds in rat brain membranes, based on a competitive binding assay using 3H-saxitoxin; the dissociation constants of 4-epiTTX and 4,9-anhydroTTX were 38- and 100-fold larger, respectively, than that of TTX [6].
It has also been reported that conversion between TTX, 4-epiTTX and 4,9-anhydroTTX occurred in living, cultured, juvenile pufferfish (kusafugu, Takifugu niphobles) [53]. The latter authors separately administered almost pure TTX, 4-epiTTX or 4,9-anhydroTTX to the test fish by intramuscular injection. When pure TTX was administered, the ratio of TTX to 4,9-anhydroTTX was ~5:4 (mol/mol) 4 days after injection. Moreover, administration of pure 4,9-anhydroTTX resulted in a ~1:5 ratio (mol/mol) of TTX to 4,9-anhydroTTX, suggesting that TTX is rapidly converted to 4,9-anhydroTTX in living pufferfish, whereas conversion of 4,9-anhydroTTX to TTX occurs at a somewhat slower rate.
It has been reported previously that 4,9-anhydroTTX can react with a large excess of thiol compounds, such as cysteine, reduced glutathione (GSH) and mercaptoethanol (Figure 4; [54]). The hemiaminal ether carbon C-4 present in 4,9-anhydroTTX can easily accept nucleophiles. In fact, the cysteine adduct of TTX, 4-S-cysteinylTTX, has been isolated from the liver of the pufferfish, Takifugu pardalis, as a probable metabolite of TTX. This finding suggests that there is an abundance of 4,9-anhydroTTX and cysteine in the pufferfish liver. Indeed, the ratio of 4,9-anhydroTTX to total TTX analogues in the liver of the pufferfish is significantly larger than that in the ovary, although the reason for this specific distribution of 4,9-anhydroTTX in the liver remains unknown. The nucleophilic addition of thiols to the C-4 in 4,9-anhhydroTTX proceeds easily under slightly alkaline conditions (pH 8.0) depending on the pKa of the thiol groups (pKa of cysteine, 8.3–8.5). However, TTX barely reacts with thiol compounds, probably because the hemiaminal carbon (C-4) in TTX is less reactive than that in 4,9-anhydroTTX; a 4,9-ether would likely be a better leaving group than a 4-hydroxy group. In addition, the thiol adducts of TTX, 4-S-cysteinylTTX and 4-S-glutathionylTTX (4-GSTTX), are not stable under near-physiological conditions, with hydrolysis to 4,9-anhydroTTX in the presence of thiol compounds occurring readily in 0.8 M sodium phosphate buffer at pH 8 and 25 °C. Therefore, detoxification of TTX with thiols may not prove to be successful, even though 4-S-cysteinylTTX is almost non-toxic to mice.
It is notable that the total chemical synthesis of TTX analogues [55] usually involves the generation of 4,9-anhydro compounds until nearly the final step; the 4,9-anhydro compounds are then derived to the relevant TTX analogues by hydrolysis of the 4,9-ether bonds in aqueous trifluoroacetic acid. This reaction arises because the 4,9-ether bonds stabilize TTX-related molecules, allowing them to survive different reaction conditions as these complex molecules are synthesized. Similarly, we have predicted recently that 4,9-anhydroTTX is the immediate precursor of TTX in the biosynthesis of TTX, although the total TTX biosynthetic pathway has not yet been fully elucidated [52].
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Figure 4. Formation and hydrolysis of the 4-thiol adduct of TTX.
Figure 4. Formation and hydrolysis of the 4-thiol adduct of TTX.
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6. Concluding Remarks

The present review has provided information concerning 4,9-anhydroTTX, a TTX analogue purified from crude TTX, and described the molecular and physiological properties of its target NaV1.6 channel. It is well known that NaV1.6 channels play an important role in pain and the development of cancer and NaV1.6 channels have also been implicated in both normal and pathological pain [17]. In future, highly selective agents for NaV1.6 channels may prove clinically useful in the relief of pain and the prevention of cancer growth. Based on the findings to date, it is plausible that 4,9-anhydroTTX, a highly isoform-selective TTX analogue, will turn out to be an invaluable research tool not only for the identification of NaV1.6 channel-mediated functions but also the development of drugs for therapeutic interventions. However, 4,9-anhydroTTX may contain a small amount of TTX due to chemical equilibrium when kept in solution. TTX analogues show promise as tools for clinical research, but conclusive results have yet to be published. Further studies are required to determine the stability of 4,9-anhydroTTX in a crude mixture of TTX analogues.

Acknowledgments

This research was supported by a KAKENHI Grant-in-Aid for Scientific Research (B) from the Japanese Society for the Promotion of Science (Noriyoshi Teramoto, Grant Number 26282148) and a KAKENHI Grant-in-Aid for Scientific Research (B) from the Japanese Society for the Promotion of Science (Mari Yotsu-Yamashita, Grant Number 26292057). This research was also supported by a Funding Program for Next Generation World-Leading Researchers (Mari Yotsu-Yamashita, Grant Number LS012; Noriyoshi Teramoto, Grant Number LS096) from the Japanese Society for the Promotion of Science.

Author Contributions

N.T. and M.Y.-Y. contributed to the concept of the study and the manuscript preparation. N.T. and M.Y.-Y. drafted and wrote the manuscript. All authors approved the final version of the manuscript.

Conflicts of Interest

The authors declare no conflict of interest.

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MDPI and ACS Style

Teramoto, N.; Yotsu-Yamashita, M. Selective Blocking Effects of 4,9-Anhydrotetrodotoxin, Purified from a Crude Mixture of Tetrodotoxin Analogues, on NaV1.6 Channels and Its Chemical Aspects. Mar. Drugs 2015, 13, 984-995. https://doi.org/10.3390/md13020984

AMA Style

Teramoto N, Yotsu-Yamashita M. Selective Blocking Effects of 4,9-Anhydrotetrodotoxin, Purified from a Crude Mixture of Tetrodotoxin Analogues, on NaV1.6 Channels and Its Chemical Aspects. Marine Drugs. 2015; 13(2):984-995. https://doi.org/10.3390/md13020984

Chicago/Turabian Style

Teramoto, Noriyoshi, and Mari Yotsu-Yamashita. 2015. "Selective Blocking Effects of 4,9-Anhydrotetrodotoxin, Purified from a Crude Mixture of Tetrodotoxin Analogues, on NaV1.6 Channels and Its Chemical Aspects" Marine Drugs 13, no. 2: 984-995. https://doi.org/10.3390/md13020984

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