6-Bromohypaphorine from Marine Nudibranch Mollusk Hermissenda crassicornis is an Agonist of Human α7 Nicotinic Acetylcholine Receptor
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Structure Determination for 6-Bromohypaphorine from Hermissenda crassicornis
2.2. Two-Electrode Voltage-Clamp
2.3. Radioligand Analysis
2.4. Calcium Imaging
2.5. Discussion
3. Experimental Section
3.1. Isolation and Structure Determination of l-6-Bromohypaphorine
3.1.1. Animal Material and Isolation of 6-BHP
3.1.2. Physical Characteristics and Spectral Data fo 6-BHP
3.2. Heterologous Expression of the nAChR Subtypes in Xenopus Oocytes and Electrophysiology Measurements
3.3. Heterlogous Expression of the α7 nAChR in Neuroblastoma Neuro2a Cell Line and Ca2+ Measurements in Response to Agonists and Antagonists
3.4. Radioligand Assay
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Kasheverov, I.E.; Shelukhina, I.V.; Kudryavtsev, D.S.; Makarieva, T.N.; Spirova, E.N.; Guzii, A.G.; Stonik, V.A.; Tsetlin, V.I. 6-Bromohypaphorine from Marine Nudibranch Mollusk Hermissenda crassicornis is an Agonist of Human α7 Nicotinic Acetylcholine Receptor. Mar. Drugs 2015, 13, 1255-1266. https://doi.org/10.3390/md13031255
Kasheverov IE, Shelukhina IV, Kudryavtsev DS, Makarieva TN, Spirova EN, Guzii AG, Stonik VA, Tsetlin VI. 6-Bromohypaphorine from Marine Nudibranch Mollusk Hermissenda crassicornis is an Agonist of Human α7 Nicotinic Acetylcholine Receptor. Marine Drugs. 2015; 13(3):1255-1266. https://doi.org/10.3390/md13031255
Chicago/Turabian StyleKasheverov, Igor E., Irina V. Shelukhina, Denis S. Kudryavtsev, Tatyana N. Makarieva, Ekaterina N. Spirova, Alla G. Guzii, Valentin A. Stonik, and Victor I. Tsetlin. 2015. "6-Bromohypaphorine from Marine Nudibranch Mollusk Hermissenda crassicornis is an Agonist of Human α7 Nicotinic Acetylcholine Receptor" Marine Drugs 13, no. 3: 1255-1266. https://doi.org/10.3390/md13031255