Next Article in Journal
Food Fortification and Decline in the Prevalence of Neural Tube Defects: Does Public Intervention Reduce the Socioeconomic Gap in Prevalence?
Previous Article in Journal
Do Quiet Areas Afford Greater Health-Related Quality of Life than Noisy Areas?
Previous Article in Special Issue
Use of a Deuterated Internal Standard with Pyrolysis-GC/MS Dimeric Marker Analysis to Quantify Tire Tread Particles in the Environment
Int. J. Environ. Res. Public Health 2013, 10(4), 1304-1311; doi:10.3390/ijerph10041304
Article

Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes

1,2
,
2
,
3
,
1,2,3
,
2,3
,
1,2
,
1,2,4
 and
1,2,*
1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic 2 Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic 3 Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Palackeho 1-3, CZ-612 42 Brno, Czech Republic 4 Lead and Cadmium Initiatives, United Nations Environment Program, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic
* Author to whom correspondence should be addressed.
Received: 24 January 2013 / Revised: 13 March 2013 / Accepted: 20 March 2013 / Published: 28 March 2013
(This article belongs to the Special Issue Mass Spectrometry and Environmental Analysis)
View Full-Text   |   Download PDF [221 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

In this study, in vitro formed Cd-phytochelatin (PC2) complexes were characterized using ion exchange chromatography (IEC) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The ratio of both studied compounds as well as experimental conditions were optimized. The highest yield of the complex was observed under an applied concentration of 100 µg·mL−1 PC2 and 100 µg·mL−1 of CdCl2. The data obtained show that IEC in combination with MALDI-TOF is a reliable and fast method for the determination of these complexes.
Keywords: ion exchange chromatography; mass spectrometry; MALDI-TOF; phytochelatin; cadmium; intramolecular complex ion exchange chromatography; mass spectrometry; MALDI-TOF; phytochelatin; cadmium; intramolecular complex
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Rodrigo, M.A.M.; Cernei, N.; Kominkova, M.; Zitka, O.; Beklova, M.; Zehnalek, J.; Kizek, R.; Adam, V. Ion Exchange Chromatography and Mass Spectrometric Methods for Analysis of Cadmium-Phytochelatin (II) Complexes. Int. J. Environ. Res. Public Health 2013, 10, 1304-1311.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert