Next Article in Journal
The Association of Lone-Motherhood with Smoking Cessation and Relapse: Prospective Results from an Australian National Study
Next Article in Special Issue
The Natural History of IgE-Mediated Food Allergy: Can Skin Prick Tests and Serum-Specific IgE Predict the Resolution of Food Allergy?
Previous Article in Journal
Scoping the Impact of Changes in Population Age-Structure on the Future Burden of Foodborne Disease in The Netherlands, 2020–2060
Article Menu

Export Article

Open AccessArticle
Int. J. Environ. Res. Public Health 2013, 10(7), 2897-2905; doi:10.3390/ijerph10072897

Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food

State Key Laboratory of Food Science & Technology, School of Food Science & Technology, Jiangnan University, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Received: 6 May 2013 / Revised: 17 June 2013 / Accepted: 3 July 2013 / Published: 12 July 2013
(This article belongs to the Special Issue Food Allergy, Genes and Environment)
View Full-Text   |   Download PDF [160 KB, uploaded 19 June 2014]   |  

Abstract

We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content. View Full-Text
Keywords: peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA peanut allergen; Ara h 1; monoclonal antibody; sandwich ELISA
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Peng, J.; Song, S.; Xu, L.; Ma, W.; Liu, L.; Kuang, H.; Xu, C. Development of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food. Int. J. Environ. Res. Public Health 2013, 10, 2897-2905.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Environ. Res. Public Health EISSN 1660-4601 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top